TWI671013B - Frozen yogurt and method for preparing thereof - Google Patents

Frozen yogurt and method for preparing thereof Download PDF

Info

Publication number
TWI671013B
TWI671013B TW106136989A TW106136989A TWI671013B TW I671013 B TWI671013 B TW I671013B TW 106136989 A TW106136989 A TW 106136989A TW 106136989 A TW106136989 A TW 106136989A TW I671013 B TWI671013 B TW I671013B
Authority
TW
Taiwan
Prior art keywords
ice cream
yogurt
weight
group
soursop
Prior art date
Application number
TW106136989A
Other languages
Chinese (zh)
Other versions
TW201916805A (en
Inventor
呂英震
陳燕萍
Original Assignee
國立嘉義大學
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 國立嘉義大學 filed Critical 國立嘉義大學
Priority to TW106136989A priority Critical patent/TWI671013B/en
Publication of TW201916805A publication Critical patent/TW201916805A/en
Application granted granted Critical
Publication of TWI671013B publication Critical patent/TWI671013B/en

Links

Landscapes

  • Confectionery (AREA)
  • Dairy Products (AREA)

Abstract

一種優格冰淇淋,其包括10重量%至25重量%的牛奶、5重量%至20重量%山刺番荔枝( Annona muricata)果肉、27重量%的優格、12重量%的甜味料、13重量%的蛋及18重量%的奶油。於此,在優格冰淇淋中的山刺番荔枝果肉可以取代各種乳化劑或安定劑,使優格冰淇淋具有低融化速率,亦可增加優格冰淇淋中所含的益生菌的存活率及生長率、對胃酸及膽鹼的耐受性,進而使優格冰淇淋的營養價值大幅提升。 A yogurt ice cream comprising 10 to 25% by weight of milk, 5 to 20% by weight of Annona muricata pulp, 27% by weight of Yogurt, 12% by weight of sweetener, 13 Eggs by weight and 18% by weight cream. Here, the Soursop fruit in Yogurt ice cream can replace various emulsifiers or stabilizers, so that Yogurt ice cream has a low melting rate, and it can also increase the survival rate and growth rate of probiotics contained in Yogurt ice cream Tolerance to gastric acid and choline, which in turn greatly enhances the nutritional value of yogurt.

Description

優格冰淇淋及其製作方法Yogurt ice cream and its making method

本發明關於一種食品製造技術,特別是關於一種優格冰淇淋及其製作方法。The invention relates to a food manufacturing technology, in particular to a yogurt ice cream and a preparation method thereof.

冰淇淋是現今社會大眾普遍喜愛的食品。依美國聯邦標準 (USDA)的之定義:冰淇淋應含至少 10% 乳脂 (Milk fat)及20%總乳固形物(Total milk solid),除含一般香料外,脂肪及總乳固形物分別不得少於8%及16%。冰淇淋整體總重量以及總固形物(Total food solid)分別至少有0.54公斤/公升及0.19公斤/公升。而依據食用冰品工廠專則,冰淇淋類食品的定義為:以牛乳或乳製品為主原料,添加糖、乳化劑、安定劑、香料等於主原料中並調配成液狀物。將液狀物凍結處理成如冰淇淋、霜淇淋、雪波(Sherbet)、雪糕等固體食品。冰淇淋乳脂不低於8%,總乳固形物不低於16%;而雪波係乳成分較低者,乳脂1至2%,總乳固形物 2至5%。如依硬度區分,冰淇淋的種類可分為硬質冰淇淋(Hard ice cream)及軟質冰淇淋(或稱霜淇淋,Soft ice cream)。硬質冰淇淋是指一般置於冰櫃內的冰淇淋,而軟質冰淇淋是於製造過程省略硬化程序而製成的冰淇淋。如依特定組成物濃度區分,冰淇淋可大致分為:冷凍卡士達、原味冰淇淋、調味冰淇淋、冰乳、冷凍果汁、冰品、仿製冰淇淋等。但一般冰淇淋具有非常高的熱量,因此市場上出現優格冰淇淋等低熱量產品,並逐漸取代一般的冰淇淋。Ice cream is a popular food in the society today. According to the definition of the United States Federal Standards (USDA): ice cream should contain at least 10% Milk fat and 20% Total milk solids. In addition to containing general flavors, fat and total milk solids must be indispensable. At 8% and 16%. The total weight of the ice cream and the total food solids are at least 0.54 kg / liter and 0.19 kg / liter, respectively. According to the edible ice factory rules, ice cream foods are defined as: milk or dairy products are the main raw materials, and sugar, emulsifiers, stabilizers, and spices are added to the main raw materials and formulated into a liquid. The liquid is frozen and processed into solid foods such as ice cream, cream, Sherbet, and ice cream. The ice cream has a milk fat of not less than 8% and a total milk solids of not less than 16%; while the snow wave milk component is lower, the milk fat is 1 to 2% and the total milk solids is 2 to 5%. According to the hardness, the types of ice cream can be divided into hard ice cream and soft ice cream. Hard ice cream refers to ice cream generally placed in a freezer, while soft ice cream is ice cream made by omitting the hardening process in the manufacturing process. According to the concentration of specific composition, ice cream can be roughly divided into: frozen casta, original ice cream, flavored ice cream, ice cream, frozen fruit juice, ice products, imitation ice cream and so on. However, generally ice cream has a very high heat content, so low-calorie products such as Yogurt ice cream appear on the market, and gradually replace general ice cream.

優格含有對人體有益的益生菌,例如:乳酸菌,其有助於抑制致病菌維持腸道內菌叢之平衡、促進胃腸道健康、緩和乳糖不耐症、增近膽鹽之去結合作用及降低膽固醇、調節宿主免疫系統、以及抗腫瘤。然而,在冰淇淋的冷凍過程中,所含有的益生菌可能會受到機械性損傷、溶質損傷效應的影響而降低其存活率,甚至部分菌種由於本身的細胞形態、尺寸大小等特性而容易在冷凍環境下損傷或死亡。Yogurt contains probiotics that are beneficial to the human body, such as lactic acid bacteria, which help to inhibit pathogenic bacteria to maintain the balance of the intestinal flora, promote gastrointestinal health, alleviate breast milk tolerance, and increase the debinding effect of bile salts. And reduce cholesterol, regulate the host's immune system, and anti-tumor. However, during the freezing process of ice cream, the probiotics contained in it may be affected by mechanical damage and solute damage to reduce its survival rate, and even some bacteria are easily frozen due to their own cell morphology, size and other characteristics. Damage or death in the environment.

在一實施例中,一種優格冰淇淋,其可包含10~25重量%的牛奶、5~20重量%的山刺番荔枝果肉、27重量%的優格、12重量%的甜味料、13重量%的蛋及18重量%的奶油。In one embodiment, a yogurt ice cream may include 10-25% by weight of milk, 5-20% by weight of Soursop fruit pulp, 27% by weight of Yogurt, 12% by weight of sweetener, 13% by weight. Eggs by weight and 18% by weight cream.

在一實施例中,一種優格冰淇淋的製作方法,其可包含將蛋與甜味料混合成一第一混合物、將牛奶及奶油加入至第一混合物中以混合成第二混合物、加熱第二混合物、以調理棒攪拌加熱後的第二混合物3分鐘以形成均質物、將均質物於4℃熟成16小時、將山刺番荔枝果肉及優格添加至經熟成的均質物形成冰淇淋混料、以及以攪凍機攪凍至冰淇淋混料呈現濃稠膏狀。並且,上述冰淇淋混料具有10重量%至25重量%的牛奶、5重量%至20重量%的山刺番荔枝果肉、27重量%的優格、12重量%的甜味料、13重量%的蛋與18重量%的奶油。In one embodiment, a method for making yogurt ice cream may include mixing eggs and sweeteners into a first mixture, adding milk and cream to the first mixture to mix into a second mixture, and heating the second mixture. 2. Stir the heated second mixture with a conditioning rod for 3 minutes to form a homogeneous substance, mature the homogeneous substance at 4 ° C for 16 hours, add the mangosteen fruit pulp and yogurt to the cooked homogeneous substance to form an ice cream mix, Frozen with ice creamer until the ice cream mix appears thick. In addition, the above ice cream mix has 10 to 25% by weight of milk, 5 to 20% by weight of Soursop fruit pulp, 27% by weight of Yogurt, 12% by weight of sweeteners, and 13% by weight of Egg with 18% by weight cream.

總言之,本發明實施例的優格冰淇淋具有適當含量的山刺番荔枝果肉,並具備良好的外觀、香氣、風味與質地。山刺番荔枝果肉具有豐富的多醣,可降低優格冰淇淋本身的融化速率,而使其具有更佳的口感。並且,山刺番荔枝果肉的多醣不僅可避免優格冰淇淋中的益生菌在冷凍環境降低存活率,促進乳酸菌的生長而使乳酸菌的數量增加,還可使提升益生菌在人體消化系統中高胃酸、高膽鹼的環境下維持其生理作用。因此,在本發明實施例的優格冰淇淋中的山刺番荔枝果肉可提供相當於各種市售乳化劑或安定劑的作用,亦使本發明實施例的冰淇淋可保持更為優異的營養性質。In summary, the yogurt ice cream according to the embodiment of the present invention has an appropriate content of mangosteen fruit pulp, and has good appearance, aroma, flavor and texture. Soursop fruit pulp is rich in polysaccharides, which can reduce the melting rate of Yogurt ice cream itself, so that it has a better taste. In addition, the polysaccharides from the fruit pulp of Soursop can not only prevent the probiotic bacteria in Yogurt ice cream from reducing the survival rate in a frozen environment, but also promote the growth of lactic acid bacteria and increase the number of lactic acid bacteria. Maintains its physiological effects in a high choline environment. Therefore, the Soursop fruit pulp in Yogurt ice cream according to the embodiment of the present invention can provide an effect equivalent to various commercially available emulsifiers or stabilizers, and also enables the ice cream of the embodiment of the present invention to maintain more excellent nutritional properties.

在一些實施例中,一種優格冰淇淋可包括牛奶、山刺番荔枝果肉、優格、甜味料、蛋及奶油。並且,以優格冰淇淋的整體重量為基礎,牛奶的含量為10重量%至25重量%,山刺番荔枝果肉的含量為5重量%至20重量%,優格的含量為27重量%,甜味料的含量為12重量%,蛋的含量為13重量%,且奶油的含量為18重量%。In some embodiments, a yogurt yogurt may include milk, soursop fruit pulp, yogurt, sweeteners, eggs, and cream. And based on the overall weight of Yogurt ice cream, the content of milk is 10% to 25% by weight, the content of Soursop fruit is 5% to 20% by weight, and the content of Yogurt is 27% by weight. The content of the flavor was 12% by weight, the content of eggs was 13% by weight, and the content of cream was 18% by weight.

在一些實施例中,蛋可為全蛋或蛋黃,且較佳地為蛋黃。In some embodiments, the egg may be a whole egg or an egg yolk, and is preferably an egg yolk.

在一些實施例中,山刺番荔枝果肉在優格冰淇淋中所佔的含量可為5重量%至10重量%。並且,在一些實施例中,山刺番荔枝果肉在優格冰淇淋中所佔的含量為5重量%。In some embodiments, the content of Soursop fruit pulp in Yogurt ice cream may be 5% to 10% by weight. And, in some embodiments, the content of Soursop fruit pulp in Yogurt ice cream is 5% by weight.

在一些實施例中,優格可包括至少一種的益生菌菌種,例如:嗜酸乳桿菌( Lactobacillus acidophilus)、乾酪乳酸桿菌( Lactobacillus casei)、保加利亞乳桿菌( Lactobacillus delbrueckiissp. Bulgaricus)、彎曲乳桿菌 ( Lactobacillus curvatus)、酵素乳桿菌(Lactobacillus fermentum)、乳酸乳桿菌(Lactobacillus lactis)、胚芽乳酸菌( Lactobacillus plantarum)、洛德乳桿菌( Lactobacillus reuteri)、短乳桿菌( Lactobacillus bervis)等。在一些實施例中,優格可包括9.59 log CFU/毫升至9.67 log CFU/毫升的保加利亞乳酸桿菌及/或9.70 log CFU/毫升至9.82 log CFU/毫升的嗜熱鏈球菌。 In some embodiments, Yogurt can include at least one type of probiotic bacteria, such as: Lactobacillus acidophilus , Lactobacillus casei , Lactobacillus delbrueckii ssp. Bulgaricus , curved milk Lactobacillus curvatus , Lacobacillus fermentum, Lacobacillus lactis, Lactobacillus plantarum , Lactobacillus reuteri , Lactobacillus bervis , etc. In some embodiments, Yogurt may include Lactobacillus bulgaricus from 9.59 log CFU / ml to 9.67 log CFU / ml and / or Streptococcus thermophilus from 9.70 log CFU / ml to 9.82 log CFU / ml.

在一些實施例中,優格冰淇淋的黏度可為343厘泊(centipoise, cp)至1422厘泊。In some embodiments, the viscosity of Yogurt ice cream can be from 343 centipoise (cp) to 1422 centipoise.

在一些實施例中,優格冰淇淋的融化速率可為0.86克/分鐘至1.06克/分鐘。In some embodiments, the melting rate of the yogurt ice cream may be 0.86 g / min to 1.06 g / min.

參照圖1,在一些實施例中,優格冰淇淋的製作方法可包括以下流程。首先,將蛋與甜味料混合成第一混合物(步驟S11)。接著,將牛奶及奶油加入至第一混合物中,並且混合成第二混合物(次奏S12)。對第二混合物進行加熱(步驟S13),再以調理棒攪拌加熱後的第二混合物約3分鐘,以形成均質物(步驟S14)。將均質物在4℃的環境下熟成16小時(步驟S15),且將山刺番荔枝果肉及優格添加至精熟成的均質物,以形成冰淇淋混料(步驟S16)。最後,以攪凍機攪凍冰淇淋混料,使冰淇淋混料呈現濃稠的膏狀(步驟S17)。Referring to FIG. 1, in some embodiments, a method for making yogurt ice cream may include the following processes. First, the egg and sweetener are mixed into a first mixture (step S11). Next, milk and cream are added to the first mixture and mixed into a second mixture (Sue S12). The second mixture is heated (step S13), and the heated second mixture is stirred with a conditioning rod for about 3 minutes to form a homogeneous material (step S14). The homogenate is aged at 4 ° C. for 16 hours (step S15), and Soursop fruit pulp and yogurt are added to the refined homogenate to form an ice cream mixture (step S16). Finally, the ice cream mixture is frozen with a thawing machine, so that the ice cream mixture has a thick paste shape (step S17).

在一些實施例中,可於小於或等於­20℃下硬化經攪凍的冰淇淋混料。In some embodiments, the frozen ice cream mix may be hardened at less than or equal to ­20 ° C.

以下將藉由所列舉的實施例配合隨附圖式,以詳細說明本發明之技術內容及特徵。In the following, the technical content and features of the present invention will be described in detail through the listed embodiments in conjunction with the accompanying drawings.

一、優格冰淇淋的製作 First, the production of yogurt .

將山刺番荔枝(由台南關子嶺及南西農民提供)削皮,並利用濾網取得山刺番荔枝果肉。將所得的山刺番荔枝果肉以95℃加熱 10 分鐘後,放入冷凍庫中保存以供後續使用。並且,以商業菌種接菌至全脂鮮奶中於 37℃發酵至pH 4.5±0.02以獲得優格。Peel the mountain soursop (provided by farmers in Guanziling, Tainan, South) and use a strainer to obtain the fruit. After heating the obtained Soursop fruit pulp at 95 ° C for 10 minutes, it is stored in a freezer for subsequent use. In addition, commercial strains were inoculated into fresh whole milk and fermented to pH 4.5 ± 0.02 at 37 ° C to obtain yogurt.

接著,將蛋黃液與細砂糖充混合後,加入全脂鮮奶及鮮奶油加熱至60℃均勻混合。並且,以80℃加熱10分鐘再以手持式攪拌棒(TCY-6709,大家源)高速攪拌3分鐘形成冰淇淋混料。將混料冷卻並於4℃熟成16小時,再將經由上述流程所準備的山刺番荔枝果肉與優格加入至經過熟成的冰淇淋混料,以全自動冰淇淋機(282603,荷蘭公主)攪凍至冰淇淋混料呈現濃稠的膏狀後裝入適當的容器中,於­20℃下硬化後得到優格冰淇淋。在優格冰淇淋中各成分所佔的含量為如表1所示。Next, after mixing the egg yolk liquid with fine sugar, add whole milk and fresh cream and heat to 60 ° C. to mix uniformly. In addition, it was heated at 80 ° C for 10 minutes, and then stirred at high speed for 3 minutes with a hand-held stirring bar (TCY-6709, every source) to form an ice cream mixture. The mixture is cooled and matured at 4 ° C for 16 hours, and then the Soursop fruit pulp and Yogurt prepared through the above process are added to the matured ice cream mixture, and thawed by a fully automatic ice cream machine (282603, Dutch princess). After the ice cream mixture shows a thick paste, it is filled into a suitable container, and then hardened at 20 ° C to obtain a yogurt. The content of each component in Yogurt ice cream is shown in Table 1.

所製備的優格冰淇淋包括不含山刺番荔枝果肉的優格冰淇淋(即,第一組)、含有5重量%山刺番荔枝果肉的優格冰淇淋(即,第二組)、 含有10%山刺番荔枝果肉的優格冰淇淋(即,第三組)、含有15%山刺番荔枝果肉的優格冰淇淋(即,第四組)以及含有20%山刺番荔枝果肉的優格冰淇淋(即,第五組)。The prepared Yogurt ice cream includes Yogurt ice cream (i.e., the first group) without succulent fruit pulp, Yogurt ice cream (i.e., the second group) with 5% by weight of succulent fruit pulp, and 10% mountain Yogurt ice cream (i.e. group 3) with soursop fruit pulp, Yogurt ice cream (i.e. group 4) with 15% soursop fruit pulp, and Yogurt ice cream (i.e. , Fifth group).

表1 成分 第一組 第二組 第三組 第四組 第五組 全脂鮮奶 30 25 20 15 10 細砂糖 12 12 12 12 12 蛋黃液 13 13 13 13 13 鮮奶油 18 18 18 18 18 優格 27 27 27 27 27 山刺番荔枝果肉 5 10 15 20 (單位:%) Table 1 ingredient First group Second Group The third group Fourth group Fifth group Whole milk 30 25 20 15 10 Fine sugar 12 12 12 12 12 Egg yolk 13 13 13 13 13 fresh cream 18 18 18 18 18 Yogurt 27 27 27 27 27 Soursop Fruit 5 10 15 20 (unit:%)

二、two, 優格的益生菌菌數分析。Analysis of Yogurt Probiotics.

利用MRS液體培養基(購自美國Difco公司)及M17液體培養基(購自美國 Difco公司)分別與洋菜粉混合以製備成用MRS固體培養基及M17固體培養基。自上述流程得到的優格中取1克加入至9毫升的0.85%(w/v)生理食鹽水且混合均勻後,依序做10倍序列稀釋而得到稀釋優格溶液。取0.1毫升的稀釋優格溶液塗抹於 MRS固體培養基及M17固體培養基,於37℃恆溫箱培養48小時。完成培養後選取具有約25至250個菌落的培養基,計算培養基上的乳酸菌菌數及嗜熱鏈球菌菌數,所得的數據以 CFU/毫升為單位表示。MRS liquid medium (purchased from American Difco) and M17 liquid culture (purchased from American Difco) were mixed with agar powder to prepare MRS solid medium and M17 solid medium. 1 g of Yogurt obtained from the above process was added to 9 ml of 0.85% (w / v) physiological saline and mixed uniformly, and a 10-fold serial dilution was performed in order to obtain a diluted Yogurt solution. 0.1 ml of diluted Yogurt solution was smeared on MRS solid medium and M17 solid medium, and cultured in a 37 ° C incubator for 48 hours. After the culture is completed, a medium having about 25 to 250 colonies is selected, and the number of lactic acid bacteria and the number of Streptococcus thermophilus bacteria on the medium are calculated. The obtained data is expressed in units of CFU / ml.

三、優格冰淇淋的特性分析。Third, the characteristics of Yogurt ice cream.

對第一組至第五組的優格冰淇淋(如表1所示)分別進行下列項目的分析,以評估其理化性質、安定性、抗氧化能力、以及其所含的益生菌的耐胃酸能力與耐膽鹽能力,並對各組優格冰淇淋進行感官品評。Analyze the following items for Yogurt Ice Cream (shown in Table 1) of the first to fifth groups to evaluate their physical and chemical properties, stability, antioxidant capacity, and gastric acid resistance of the probiotics contained in them And bile salt tolerance, sensory evaluation of each group of yogurt.

1.1. 酸鹼值:PH value:

將酸鹼度計以pH 4.01及pH 7.01標準液校正後,對第一組至第五組優格冰淇淋進行酸鹼值測定。After the pH meter was calibrated with standard solutions of pH 4.01 and pH 7.01, the pH values of the first to fifth groups of yogurt ice cream were measured.

2.2. 黏度:Viscosity:

從第一組至第五組優格冰淇淋取出並分別將其融化成液體狀。每個液體狀的優格冰淇淋(即,冰淇淋漿)取200毫升,在5℃下以轉速設定為10rpm且配備2號探針的黏度計(Model dv–Ⅱ,Brookfiled)對每個冰淇淋漿量測黏度。Take out Yogurt ice cream from the first to fifth groups and melt them into liquids. Take 200 ml of each liquid yogurt ice cream (ie, ice cream syrup), and set the speed at 10 rpm at 5 ° C with a viscometer (Model dv-II, Brookfiled) equipped with a probe No. 2 for each ice cream syrup. Measure viscosity.

3.3. 膨脹率:Expansion rate:

將前述每個冰淇淋漿秤重。之後,將每個冰淇淋漿再凍結回固體狀後,對每個固體狀的優格冰淇淋(即,軟質冰淇淋)秤重。以下式1計算第一組至第五組的優格冰淇淋的膨脹率。 膨脹率=(等重量的冰淇淋漿的體積-等重量的軟質冰淇淋的體積/等重量的軟質冰淇淋的體積)×100%...(式1)。Weigh each of the aforementioned ice cream syrups. After that, each ice cream syrup is frozen back to a solid state, and then each solid yogurt (ie, soft ice cream) is weighed. The expansion ratio of Yogurt ice cream of the first group to the fifth group is calculated by the following Equation 1. Expansion rate = (volume of ice cream slurry of equal weight-volume of soft ice cream of equal weight / volume of soft ice cream of equal weight) × 100% ... (Formula 1).

4.4. 融化率:Melting rate:

從前述每個軟質冰淇淋中秤取50克,並移入溫度為-20℃的冷凍庫,以將軟質冰淇淋硬化成呈現尚未融化成冰淇淋漿的原始狀態的優格冰淇淋。硬化完成後,將每組優格冰淇淋置於25℃的恆溫箱中再次進行融化。每隔5分鐘對置於恆溫箱中的優格冰淇淋秤重。以下列式2計算第一組至第五組的優格冰淇淋的融化速率。 融化速率=(恆溫箱中的優格冰淇淋的重量/融化時間)…(式2)。50 grams were weighed from each of the aforementioned soft ice creams, and transferred into a freezer at a temperature of -20 ° C to harden the soft ice creams into a yogurt ice cream that showed an original state that had not yet melted into ice cream syrup. After the hardening is completed, each group of Yogurt ice cream is melted again in a 25 ° C incubator. Weigh Yogurt in the incubator every 5 minutes. Calculate the melting rate of Yogurt Ice Cream from the first group to the fifth group using Equation 2 below. Melting rate = (weight of yogurt ice cream in the incubator / melting time) ... (Eq. 2).

5.5. 顏色:colour:

使用色差儀(NE 4000,日本電色工業)分析第一組至第五組優格冰淇淋的L *值、a *值及b *值。L *值代表顏色的亮度,最高為100,最低為0,數值越大表示顏色越亮;a *值的正值代表紅色,負值代表綠色;b *值的正值代表黃色,負值代表藍色。 The color difference meter (NE 4000, Nippon Denshoku Industries) was used to analyze the L * value, a * value, and b * value of the first to fifth groups of yogurt ice cream. The L * value represents the brightness of the color, the highest value is 100, and the lowest value is 0. A larger value indicates a brighter color; a positive value of a * value represents red, and a negative value represents green; a positive value of b * value represents yellow, and a negative value represents blue.

6.6. 硬度及內聚性:Hardness and cohesion:

將第一組至第五組的優格冰淇淋置於-20℃的環境中。以配備有探頭P10的物性測定儀(TA.XTplus,Stable Micro Systems)測試各組優格冰淇淋的硬度及內聚性。物敬測定儀所設定的測試條件如下:偵測前速度(探頭P10的下降速度)為2毫米/秒、偵測速度為3毫米/秒、偵測後探頭P10的返回速度為5毫米/秒、偵測時施加的力為10克、探頭P10穿透深度為10毫米。Place the Yogurt ice cream in the first to fifth groups in an environment at -20 ° C. The hardness and cohesiveness of each group of Yogurt ice cream were tested with a physical property tester (TA.XTplus, Stable Micro Systems) equipped with a probe P10. The test conditions set by the Wujing tester are as follows: the speed before detection (falling speed of the probe P10) is 2 mm / second, the detection speed is 3 mm / second, and the return speed of the probe P10 after detection is 5 mm / second The force applied during detection is 10 grams, and the penetration depth of the probe P10 is 10 mm.

7.7. 優格冰淇淋的安定性:Stability of Yogurt Ice Cream:

將第一組至第五組的優格冰淇淋保存於-20℃冷凍櫃中,每間隔7日進行分析乳酸菌菌數及嗜熱鏈球菌菌數,並持續進行2個月。每次分析時,從各組優格冰淇淋取1克加入至9毫升的0.85%無菌生理食鹽水,混合均勻後依序做10倍序列稀釋以得到稀釋優格冰淇淋溶液。接著,將0.1毫升的稀釋優格冰淇淋溶液於MRS固體培養基及M17固體培養基作平板塗抹,於37℃恆溫箱培養48小時。完成培養後,選取具有約25至250個菌落的培養基,並計算其乳酸菌菌數及嗜熱鏈球菌菌數,所得的數據以 CFU/毫升為單位表示。The Yogurt ice cream of the first group to the fifth group was stored in a freezer at -20 ° C, and the number of lactic acid bacteria and the number of Streptococcus thermophilus were analyzed every 7 days, and continued for 2 months. For each analysis, take 1 gram of Yogurt ice cream from each group and add it to 9 ml of 0.85% sterile physiological saline, mix them evenly and then make a 10-fold serial dilution to obtain a diluted Yogurt ice cream solution. Next, 0.1 ml of diluted Yogurt ice cream solution was smeared on MRS solid medium and M17 solid medium as plates, and cultured in a 37 ° C incubator for 48 hours. After the culture is completed, a medium having about 25 to 250 colonies is selected, and the number of lactic acid bacteria and the number of Streptococcus thermophilus bacteria are counted. The data obtained are expressed in units of CFU / ml.

並且,將第一組至第五組的優格冰淇淋置放於室溫直至融化成液態狀,將液態狀的優格冰淇淋(冰淇淋漿)與丙酮以體積比為1:1的比例均勻混合。接著,將冰淇淋漿/丙酮混合溶液以超音波震盪30分鐘。以6000 rpm的轉速離心15分鐘,且離心後取上清液。於此,得到對應各組優格冰淇淋的冰淇淋萃取液。Furthermore, the Yogurt ice cream of the first group to the fifth group is left at room temperature until it melts into a liquid state, and the liquid yogurt ice cream (ice cream syrup) and acetone are uniformly mixed in a volume ratio of 1: 1. Next, the ice cream syrup / acetone mixed solution was ultrasonically shaken for 30 minutes. Centrifuge at 6000 rpm for 15 minutes, and take the supernatant after centrifugation. Here, an ice cream extract corresponding to each group of yogurt ice cream is obtained.

8.8. 優格冰淇淋的抗氧化能力:Antioxidant capacity of Yogurt Ice Cream:

並且,從每個冰淇淋萃取液中取出25微升,並與1毫升的2%碳酸鈉均勻混合後,避光靜置2分鐘。接著,將每個冰淇淋萃取液與碳酸鈉的混合溶液加入至250微升的50%福林酚試劑(Folin- ciocalteu’s phenol reagent)(購自Sigma-Aldrich)中並避光30分鐘形成反應液後,在波長750 nm下以分光光度計(SP-830plus,Metertech)測定各反應液的吸光值。以沒食子酸(gallic acid)(購自Alfa Aesar)作為標準品,製備不同濃度作為標準曲線,將測得的吸光值帶入此標準曲線。於此,可得到各組優格冰淇淋中總多酚的沒食子酸含量,所得的數據以微克沒食子酸當量/毫升(mg GAE (gallic acid equivalent)/ml)為單位表示。Furthermore, 25 microliters of each ice cream extract was taken out, mixed with 1 ml of 2% sodium carbonate, and left in the dark for 2 minutes. Next, add a mixed solution of each ice cream extract and sodium carbonate to 250 microliters of 50% Folin-ciocalteu's phenol reagent (purchased from Sigma-Aldrich) and form a reaction solution in the dark for 30 minutes. The absorbance of each reaction solution was measured with a spectrophotometer (SP-830plus, Metertech) at a wavelength of 750 nm. Gallic acid (purchased from Alfa Aesar) was used as a standard, and different concentrations were prepared as standard curves, and the measured absorbance values were brought into this standard curve. Here, the gallic acid content of total polyphenols in each group of Yogurt ice cream can be obtained. The obtained data is expressed in micrograms of gallic acid equivalent / ml (mg GAE (gallic acid equivalent) / ml) as a unit.

並且,從各組冰淇淋萃取液中取出200微升的冰淇淋萃取液,並與1毫升的0.2 mM 2,2-二苯基-1-苦味胼基(2,2-Diphenyl-1- picryl-hydrazyl,DPPH)溶液混合均勻並避光靜置50分鐘形成反應液後,以分光光度計在波長517 nm下測定每個反應液的吸光值。於此,以下列式3計算,可得到各組優格冰淇淋的自由基清除能力,所得的數據以清除率(%)表示。 Also, 200 microliters of ice cream extracts were taken from each group of ice cream extracts and mixed with 1 ml of 0.2 mM 2,2-diphenyl-1-picryl-hydrazyl , DPPH) solution was mixed uniformly and left to stand in the dark for 50 minutes to form a reaction solution, and the absorbance of each reaction solution was measured by a spectrophotometer at a wavelength of 517 nm. Here, the free radical scavenging ability of each group of Yogurt ice cream can be calculated by the following formula 3, and the obtained data is expressed as a clearance rate (%).

9.9. 優格冰淇淋的耐胃酸能力:Stomach acid resistance of Yogurt Ice Cream:

用1N鹽酸溶液將MRS液態培養基的pH值調整至3後進行滅菌。接著,從第一組至第五組的優格冰淇淋分別取1克加入至9毫升的MRS液態培養基中以形成第一培養液,且將各第一培養液於37℃培養3小時。完成培養後,將100微升的第一培養液進行連續稀釋,再以平板計數法對經過稀釋的第一培養液檢測乳酸菌菌數及嗜熱鏈球菌菌數。The pH of the MRS liquid culture medium was adjusted to 3 with a 1N hydrochloric acid solution, and then sterilized. Next, 1 g of Yogurt ice cream from the first group to the fifth group were respectively added to 9 ml of MRS liquid medium to form a first culture solution, and each first culture solution was cultured at 37 ° C. for 3 hours. After the culture was completed, 100 microliters of the first culture solution was serially diluted, and then the number of lactic acid bacteria and the number of Streptococcus thermophilus bacteria were detected by the plate counting method on the diluted first culture solution.

10.10. 優格冰淇淋的耐膽鹽能力:Bile salt tolerance of Yogurt ice cream:

於MRS液態培養基中添加0.4%膽鹽後進行滅菌。接著,從第一組至第五組的優格冰淇淋分別取1克加入至9毫升的MRS液態培養基中以形成第二培養液,且將各第二培養液於37℃培養3小時。完成培養後,將100微升的第二培養液進行連續稀釋,再以平板計數法對經稀釋的第二培養液檢測乳酸菌菌數及嗜熱鏈球菌菌數。Add 0.4% bile salt to MRS liquid medium and sterilize. Next, 1 g of Yogurt ice cream from the first group to the fifth group were respectively added to 9 ml of MRS liquid medium to form a second culture solution, and each second culture solution was cultured at 37 ° C. for 3 hours. After the culture was completed, 100 microliters of the second culture solution was serially diluted, and the number of lactic acid bacteria and the number of Streptococcus thermophilus bacteria were detected by the plate counting method on the diluted second culture solution.

11.11. 優格冰淇淋的感官品評:Sensory evaluation of Yogurt Ice Cream:

對第一組至第五組分別進行感官品評。針對各組優格冰淇淋的外觀色澤、香氣、味道、質地與整體喜好性進行品評,最高為9分。Sensory evaluation was performed on the first to fifth groups. According to the appearance color, aroma, taste, texture and overall preference of each group of yogurt, the highest score is 9 points.

根據上述各項試驗中所得的數據是以社會科學統計套裝軟體 (Statistical Package for Social Science, SPSS version 19.0)進行統計分析,以單因子變異數分析(One-way ANOVA)檢定各組間的差異,再以鄧肯氏多變域測定法(Duncan’s multiple rang test)作顯著性差異比較, P<0.05 即為具有顯著性差異。 According to the data obtained in the above experiments, statistical analysis was performed using the Statistical Package for Social Science (SPSS version 19.0), and one-way ANOVA was used to test the differences between the groups. Duncan's multiple rang test was used to compare the significant differences. P <0.05 was considered significant difference.

各組優格冰淇淋的pH值、酸度及顏色分析的實驗結果如表2所示。The experimental results of pH, acidity and color analysis of Yogurt ice cream in each group are shown in Table 2.

表2 組別 pH值 酸度 L* a* b* 第一組 5.23 0.22 68.35 -0.65 9.08 第二組 5.01 0.23 66.38 -0.81 9.64 第三組 4.85 0.28 66.19 -0.75 10.20 第四組 4.64 0.33 63.70 -0.80 10.60 第五組 4.54 0.39 60.13 -0.78 11.03 Table 2 Group pH value acidity L * a * b * First group 5.23 0.22 68.35 -0.65 9.08 Second Group 5.01 0.23 66.38 -0.81 9.64 The third group 4.85 0.28 66.19 -0.75 10.20 Fourth group 4.64 0.33 63.70 -0.80 10.60 Fifth group 4.54 0.39 60.13 -0.78 11.03

由表2可知,優格冰淇淋的pH值以及酸度會受到山刺番荔枝果肉的含量變化的影響。隨著山刺番荔枝果肉的含量增加,優格冰淇淋的pH值逐漸下降,而酸度逐漸上升。It can be known from Table 2 that the pH value and acidity of yogurt ice cream are affected by the change in the content of the fruit of Soursop. With the increase of the content of Soursop fruit, the pH value of Yogurt ice cream gradually decreased, while the acidity gradually increased.

優格冰淇淋的顏色表現以L*值、a*值及b*值表示。L*值表示亮度。a*值表示紅色或綠色,當a*值為正時表示紅色,而a*值為負時則為綠色。b*值表示黃色-藍色,當b*值為正時表示黃色,而b*值為負時則為藍色。The color performance of Yogurt ice cream is represented by L * value, a * value, and b * value. L * values represent brightness. The a * value indicates red or green, when a * value is positive, it means red, and when a * value is negative, it means green. The b * value indicates yellow-blue. When the b * value is positive, it means yellow, and when the b * value is negative, it means blue.

以L*值而言,優格冰淇淋的L*值隨著山刺番荔枝果肉的含量增加而顯著地變小,表示優格冰淇淋的亮度隨著山刺番荔枝果肉的含量增加而降低。第一組的 L* 值為68.35,而添加20重量%山刺番荔枝果肉(即,第五組)的 L*值為60.13,其下降幅度約為12%。In terms of L * value, the L * value of Yogurt ice cream became significantly smaller with the increase in the content of Soursop fruit, indicating that the brightness of Yogurt ice cream decreased with the increase of the content of Soursop fruit. The L * value of the first group was 68.35, and the L * value of the 20% by weight mangosteen fruit pulp (ie, the fifth group) was 60.13, and the decrease was about 12%.

以a*值而言,表2中的第一組至第五組的a*值均小於0,表示各組優格冰淇淋的呈色偏向綠色。並且,第二至五組的a*值較第一組的a*值為小,顯示添加山刺番荔枝果肉的優格冰淇淋的顏色更加偏向綠色調。In terms of a * values, the a * values of the first to fifth groups in Table 2 are all less than 0, which indicates that the color of Yogurt ice cream in each group is biased toward green. In addition, the a * value of the second to fifth groups is smaller than the a * value of the first group, which shows that the color of the yogurt ice cream with added mangosteen fruit pulp is more greenish.

以b*值而言,表2中各組的b*值均大於0,表示各組優格冰淇淋的呈色偏向黃色。並且,可發現隨著山刺番荔枝果肉的含量的增加,優格冰淇淋的b*值亦隨之上升,表示優格冰淇淋的顏色逐漸變為深黃色。由於山刺番荔枝果肉中具有約0.004微克/100克的胡蘿蔔素,因而影響b*值的變化。In terms of b * values, the b * values of each group in Table 2 are greater than 0, indicating that the color of Yogurt ice cream in each group is biased toward yellow. Moreover, it can be found that with the increase of the content of Soursop fruit, the b * value of Yogurt ice cream also rises, indicating that the color of Yogurt ice cream gradually becomes dark yellow. As the carotenoids of Soursop fruit have about 0.004 micrograms / 100 grams, the change of b * value is affected.

各組優格冰淇淋的黏度、融化速率、膨脹率、硬度及內聚性的分析結果如表3所示。Table 3 shows the analysis results of the viscosity, melting rate, expansion rate, hardness, and cohesion of Yogurt ice cream in each group.

表3 組別 黏度(cp) 融化速率(g/min) 膨脹率(%) 硬度(g) 內聚性 第一組 226 1.15 67.53 101.37 0.01542 第二組 343 1.06 62.23 187.19 0.0591 第三組 523 0.97 59.33 239.81 0.06007 第四組 1053 0.94 51.83 352.74 0.15019 第五組 1422 0.86 48.40 552.6 0.16465 table 3 Group Viscosity (cp) Melting rate (g / min) Expansion rate (%) Hardness (g) Cohesion First group 226 1.15 67.53 101.37 0.01542 Second Group 343 1.06 62.23 187.19 0.0591 The third group 523 0.97 59.33 239.81 0.06007 Fourth group 1053 0.94 51.83 352.74 0.15019 Fifth group 1422 0.86 48.40 552.6 0.16465

由表3可知,優格冰淇淋的黏度隨著山刺番荔枝果肉的含量增加而上升。由於山刺番荔枝果肉中含有大量的多醣,其可發揮相當於食品中黏稠劑的作用,從而造成優格冰淇淋的黏度上升。As can be seen from Table 3, the viscosity of the yogurt ice cream increased with the increase in the content of Soursop. Due to the large amount of polysaccharides contained in the fruit pulp of Soursop, it can play the role of a thickener in food, thus causing the viscosity of Yogurt ice cream to increase.

冰淇淋的融化速率表示冰淇淋對於溫度的耐受性。在表3中,不含山刺番荔枝果肉的優格冰淇淋(即,第一組)的融化速率為最高,而含有20重量%之山刺番荔枝果肉的優格冰淇淋(即,第五組)的融化速率為對低。隨著山刺番荔枝果肉的含量增加,融化速率亦逐漸上升。由於優格冰淇淋中的山刺番荔枝果肉含有大量多醣,且多醣可吸附水份,從而降低優格冰淇淋的融化速率。The melting rate of ice cream indicates its resistance to temperature. In Table 3, the yoghurt ice cream (ie, the first group) without the mangosteen fruit pulp has the highest melting rate, and the yoghurt ice cream (ie, the fifth group) with 20% by weight of the mangosteen fruit The melting rate is low. With the increase of the content of Soursop fruit, the melting rate also gradually increased. Because the fruit of Soursop Syrup in Yogurt Ice Cream contains a large amount of polysaccharides, and the polysaccharides can absorb water, thereby reducing the melting rate of Yogurt Ice Cream.

在製作優格冰淇淋的過程中,空氣會進入均質物及/或冰淇淋混料中,使得製成的優格冰淇淋的體積大於所有原料加總的體積。體積增加的百分率就是冰淇淋的膨脹率。膨脹率是衡量冰淇淋產品品質的重要指標。當膨脹率太高時,優格冰淇淋會呈現霜狀、疏鬆且口感不佳。從表3中可看出優格冰淇淋的膨脹率隨著山刺番荔枝果肉的含量增加而下降。由於山刺番荔枝果肉在優格冰淇淋中可發揮提高黏度的作用,而當優格冰淇淋黏度上升時,進入優格冰淇淋的空氣愈少,從而使優格冰淇淋的膨脹率變低。During the process of making yogurt, the air will enter the homogenate and / or the ice cream mixture, so that the volume of the yogurt produced is greater than the total volume of all ingredients. The percentage increase in volume is the expansion of the ice cream. Expansion rate is an important indicator to measure the quality of ice cream products. When the expansion rate is too high, Yogurt ice cream will appear frosty, loose, and taste bad. It can be seen from Table 3 that the swelling rate of Yogurt ice cream decreases with the increase in the content of Soursop. Because the fruit of Soursop can play a role in increasing the viscosity in yogurt, and when the viscosity of yogurt rises, the less air enters the yogurt, so that the expansion rate of yogurt is lower.

優格冰淇淋的質地分析結果包括硬度及內聚性的測定。由表3可知,當山刺番荔枝果肉在優格冰淇淋中的含量愈高,優格冰淇淋的硬度亦隨之增加。內聚性表示當優格冰淇淋經過兩次壓縮,其表面於第一次壓縮及第二次壓縮時為克服與探頭之間的吸引力分別所作的功的比值。由表3可知,隨著山刺番荔枝果肉在優格冰淇淋中的含量增加,優格冰淇淋的內聚性亦顯著地上升。The texture analysis results of Yogurt ice cream include determination of hardness and cohesion. It can be known from Table 3 that the higher the content of Soursop fruit pulp in yogurt, the higher the hardness of yogurt. Cohesion indicates the ratio of the work done to overcome the attractive force between the probe and the probe during the first and second compressions of the yogurt ice cream after two compressions. It can be known from Table 3 that as the content of Soursop fruit pulp in Yogurt ice cream increases, the cohesiveness of Yogurt ice cream also increases significantly.

圖2表示各組優格冰淇淋中乳酸菌的耐胃酸能力的分析結果。圖3表示各組優格冰淇淋中乳酸菌的耐膽鹽能力的分析結果。參照圖3,圖中顯示當山刺番荔枝果肉在優格冰淇淋所佔的含量為10重量%或以上(即,第二組至第五組)時,乳酸菌菌數有顯著地上升。參照圖3,圖中顯示當山刺番荔枝果肉在優格冰淇淋所佔的含量為10重量%或以上時,乳酸菌菌數有顯著地上升。Figure 2 shows the results of analysis of gastric acid tolerance of lactic acid bacteria in each group of yogurt. Figure 3 shows the results of analysis of bile salt tolerance of lactic acid bacteria in each group of yogurt. Referring to FIG. 3, the figure shows that when the content of Soursop fruit pulp in yogurt ice cream is 10% by weight or more (that is, the second group to the fifth group), the number of lactic acid bacteria significantly increases. Referring to FIG. 3, when the content of Soursop fruit pulp in yogurt ice cream is 10% by weight or more, the number of lactic acid bacteria significantly increases.

圖4及圖5分別表示在各組優格冰淇淋於-20℃的冷凍環境中放置60天的期間,乳酸菌及嗜熱鏈球菌在優格冰淇淋中的菌數隨時間的變化。由圖4及圖5可知,將各組優格冰淇淋中自第0天(即,置於冷凍環境的起始日)開始冷凍至第60天,其所含的乳酸菌及嗜熱鏈球菌的菌數大致維持恆定,顯示含有山刺番荔枝果肉的優格冰淇淋中的乳酸菌與嗜熱鏈球菌等益生菌並不會因為溫度過低而降低其存活率。Figures 4 and 5 respectively show the changes of the bacterial counts of lactic acid bacteria and Streptococcus thermophilus in Yogurt ice cream over a period of 60 days in each group of Yogurt ice cream. As can be seen from FIG. 4 and FIG. 5, the lactic acid bacteria and the Streptococcus thermophilus bacteria contained in each group of Yogurt ice cream were frozen from the 0th day (that is, the start date of being placed in the freezing environment) to the 60th day. The number remained approximately constant, indicating that probiotics such as lactic acid bacteria and Streptococcus thermophilus in yogurt yogurt containing pulp of Soursop litchi did not reduce their survival rate due to low temperature.

圖6及圖7分別表示各組優格冰淇淋中於置於-20℃的冷凍環境的起始日,乳酸菌及嗜熱鏈球菌在優格冰淇淋中的菌數變化。參照圖6,圖中顯示當優格冰淇淋含有10%的山刺番荔枝果肉(即,第二組)時,乳酸菌菌數相較於不含山刺番荔枝果肉的優格冰淇淋(即,第一組)有顯著地上升。並且,隨著山刺番荔枝果肉的含量增加,乳酸菌的菌數亦隨之增加。參照圖7,圖中顯示當優格冰淇淋含有10%的山刺番荔枝果肉(即,第二組)時,嗜熱鏈球菌的菌數相較於第一組有顯著地上升。並且,隨著山刺番荔枝果肉的含量增加,嗜熱鏈球菌的菌數亦隨之增加。由於山刺番荔枝果肉含有大量多醣,使優格冰淇淋的黏度增加。在冷凍環境下,黏度較高的優格冰淇淋可形成較少的冰晶,因而減少益生菌的細胞損傷並降低對於細胞存活率的不良影響。FIG. 6 and FIG. 7 respectively show the change of the bacterial counts of lactic acid bacteria and Streptococcus thermophilus in Yogurt ice cream on the starting day of each group of Yogurt ice cream in a freezing environment at -20 ° C. Referring to FIG. 6, the figure shows that when Yogurt ice cream contains 10% of Soursop fruit pulp (ie, the second group), the number of lactic acid bacteria is compared with that of Yogurt ice cream (ie, the first Group) have risen significantly. Moreover, with the increase of the content of Soursop fruit, the number of lactic acid bacteria also increased. Referring to FIG. 7, it is shown that when Yogurt ice cream contains 10% of Soursop fruit (that is, the second group), the number of Streptococcus thermophilus bacteria increases significantly compared to the first group. In addition, with the increase in the content of Soursop, the number of S. thermophilus bacteria increased. Due to the large amount of polysaccharides in the fruit pulp of Soursop, the viscosity of yogurt is increased. In a frozen environment, the higher viscosity Yogurt ice cream can form fewer ice crystals, thereby reducing the cell damage of probiotics and the adverse effect on cell survival.

2,2-二苯基-1-苦味胼基(DPPH)為穩定的自由基,在517 nm處有強烈的吸光。當與具有抗氧化能力的物質相互作用時,DPPH自由基接受電子而形成電子對,使其在517 nm的吸光值降低或消失。當測得的DPPH的吸光值越低,則表示此物質的抗氧化能力越強。換句話說,當物質在與DPPH自由基作用前及作用後,其吸光值變化的比率愈高,則此物質清除DPPH自由基的能力愈好,亦即抗氧化能力愈強。圖8表示為各組優格冰淇淋與DPPH自由基相互作用,並且以前述式3所計算而得的DPPH自由基的吸光值變化。由圖8可知,第一組的優格冰淇淋對於DPPH自由基清除能力最低,而第五組最高。可知當山刺番荔枝果肉在優格冰淇淋中的含量逐漸增加時,優格冰淇淋的抗氧化能力亦呈現顯著性的提升。2,2-Diphenyl-1-bitterate (DPPH) is a stable free radical with strong light absorption at 517 nm. When interacting with a substance with anti-oxidation ability, DPPH radicals accept electrons to form an electron pair, which causes its absorbance at 517 nm to decrease or disappear. The lower the measured absorbance of DPPH, the stronger the antioxidant capacity of the substance. In other words, the higher the rate of change in absorbance of a substance before and after its action with DPPH free radicals, the better its ability to scavenge DPPH free radicals, that is, the stronger its antioxidant capacity. FIG. 8 shows the DPPH radical interaction between each group of Yogurt ice cream and the change in the absorbance of DPPH radical calculated by the above formula 3. It can be seen from FIG. 8 that the yogurt ice cream of the first group has the lowest DPPH free radical scavenging ability, and the fifth group has the highest scavenging ability. It can be known that when the content of Soursop fruit pulp in Yogurt ice cream gradually increases, the antioxidant capacity of Yogurt ice cream also shows a significant improvement.

多酚化合物(Polyphenolic compounds)的苯環上具有還原端的氫氧基會與福林試劑反應而呈色,並在750 nm下具有最大吸光值。若吸光值越高,則表示總多酚含量越高。將各組優格冰淇淋與福林試劑進行反應並測其吸光值,且沒食子酸的當量為基準,利用測得的吸光值計算出優格冰淇淋的總多酚含量。圖9表示為各組優格冰淇淋與福林試劑相互作用而得的總多酚含量。由圖9可知,第一組的優格冰淇淋的總多酚含量最低,而第五組最高。可知當山刺番荔枝果肉在優格冰淇淋中的含量逐漸增加時,優格冰淇淋的總多酚含量亦增加,從而顯著地提升抗氧化能力。Polyphenolic compounds (Polyphenolic compounds) on the benzene ring with a reducing end of the hydroxyl group will react with Flynn reagent to color, and has a maximum absorbance at 750 nm. A higher light absorption value indicates a higher total polyphenol content. The Yogurt ice cream was reacted with Fulin reagent and the absorbance value of each group was measured, and the equivalent of gallic acid was used as a reference. The total polyphenol content of the Yogurt ice cream was calculated by using the measured absorbance value. Figure 9 shows the total polyphenol content obtained from the interaction between Yogurt ice cream and Fulin reagent in each group. It can be seen from FIG. 9 that the total polyphenol content of the yogurt in the first group is the lowest, and the highest in the fifth group. It can be seen that when the content of Soursop fruit pulp in Yogurt ice cream gradually increases, the total polyphenol content of Yogurt ice cream also increases, thereby significantly improving the antioxidant capacity.

圖10為各組優格冰淇淋進行感官品評的評分結果,其針對項目包含外觀、香氣、風味、質地及整體接受度。以外觀而言,各組優格冰淇淋之間無顯著的差異。以香氣而言,則是以第三組得分最高。以風味而言,第二組的得分最高,而第三組次之。於此,含有5重量%至10重量%的山刺番荔枝果肉的優格冰淇淋(即,第二組與第三組)中所具有的香氣及風味較佳。以質地而言,第五組得分最高,且隨著山刺番荔枝果肉的含量降低,質地的品評得分亦隨之降低。於此,可知當優格冰淇淋含有山刺番荔枝果肉時,有助於改善優格冰淇淋的質地,且當含量愈高時質地愈佳。以整體接受度而言,第二組得分最高,且第三組次之。於此,可知含有5重量%至10重量%的山刺番荔枝果肉的優格冰淇能夠得到較高的接受度。Figure 10 shows the results of sensory evaluation of each group of Yogurt ice cream. The items include appearance, aroma, flavor, texture, and overall acceptance. In terms of appearance, there was no significant difference between the groups of Yogurt ice cream. In terms of aroma, the third group scored the highest. In terms of flavor, the second group scored the highest, and the third group ranked second. Here, the yoghurt ice cream (ie, the second group and the third group) containing 5 to 10% by weight of Soursop fruit pulp has better aroma and flavor. In terms of texture, the fifth group scored the highest, and as the content of Soursop Litchi pulp decreased, the texture evaluation score also decreased. Here, it can be seen that when Yogurt ice cream contains Soursop fruit pulp, it helps to improve the texture of Yogurt Ice Cream, and the higher the content, the better the texture. In terms of overall acceptance, the second group scored highest, and the third group followed. Here, it can be seen that Yogurt ice cream containing 5 to 10% by weight of Soursop fruit pulp can obtain a high degree of acceptance.

綜上所述,本發明實施例的優格冰淇淋與其製造方法適用於製作一冰淇淋產品,以使所製得的優格冰淇淋能呈現良好的外觀、香氣、風味與質地。並且,藉由在優格冰淇淋中添加山刺番荔枝果肉並調整適當配方,不僅可降低優格冰淇淋中的益生菌於低溫、低pH、高酸度、高膽鹽的環境下所受的損傷,還可提高優格冰淇淋的抗氧化能力。也就是說,山刺番荔枝果肉的多醣不僅可避免優格冰淇淋中的益生菌在冷凍環境降低存活率,促進益生菌的生長而使乳酸菌的數量增加,還可使益生菌在人體消化系統中高胃酸、高膽鹼的環境下維持其生理作用,進而提升優格冰淇淋的營養性質。此外,山刺番荔枝果肉可提供優格冰淇淋較高的黏度,可降低冰淇淋本身的融化速率,使優格冰淇淋的保形性、口感與質地均十分優異。因此,在本發明實施例的冰淇淋中的山刺番荔枝果肉可提供相當於各種市售乳化劑或安定劑的作用,亦使本發明實施例的冰淇淋可保持更為優異的營養價值。In summary, the yogurt ice cream and the method for manufacturing the yogurt ice cream according to the embodiments of the present invention are suitable for making an ice cream product, so that the prepared yogurt ice cream can exhibit good appearance, aroma, flavor and texture. In addition, by adding Soursop fruit pulp to Yogurt ice cream and adjusting the appropriate formula, not only can the probiotics in Yogurt ice cream be reduced in the environment of low temperature, low pH, high acidity, high bile salt, It can also improve the antioxidant capacity of yogurt. That is to say, the polysaccharides from the fruit pulp of Soursop can not only prevent the probiotics in Yogurt ice cream from reducing the survival rate in the freezing environment, but also promote the growth of probiotics and increase the number of lactic acid bacteria. It can also make probiotics high in the human digestive system. The physiological effects of gastric acid and high choline are maintained, thereby improving the nutritional properties of yogurt. In addition, the fruit of Soursop can provide higher viscosity of Yogurt ice cream, reduce the melting rate of the ice cream itself, and make Yogurt ice cream excellent in shape retention, texture and texture. Therefore, the Soursop fruit pulp in the ice cream of the embodiment of the present invention can provide the effect equivalent to various commercially available emulsifiers or stabilizers, and the ice cream of the embodiment of the present invention can maintain more excellent nutritional value.

S11 將蛋與甜味料混合成第一混合物 S12 將牛奶及奶油加入至第一混合物中以混合成第二混合物 S13 加熱第二混合物 S14 以調理棒攪拌加熱後的第二混合物3分鐘以形成均質物 S15 將均質物於4℃熟成16小時 S16 將山刺番荔枝果肉及優格添加至經熟成的均質物形成冰淇淋混料 S17 以攪凍機攪凍至冰淇淋混料呈現濃稠膏狀S11 Mix eggs and sweeteners into a first mixture S12 Add milk and cream to the first mixture to mix into a second mixture S13 Heat the second mixture S14 Stir the heated second mixture with a conditioning rod for 3 minutes to form a homogeneous mixture S15: The homogenate is matured at 4 ° C for 16 hours. S16: Soursop fruit pulp and yogurt are added to the homogenate after ripening to form an ice cream mixture. S17 is defrosted with a blender until the ice cream mixture is thick and creamy.

圖1為本發明實施例的優格冰淇淋的製造方法的流程方塊圖。 圖2為本發明實施例的優格冰淇淋中乳酸菌的耐胃酸能力的分析結果圖。 圖3為本發明實施例的優格冰淇淋中乳酸菌的耐膽鹽能力的分析結果圖。 圖4為本發明實施例的優格冰淇淋中乳酸菌菌數相對於在-20℃環境中的儲存天數的變化曲線圖。 圖5為本發明實施例的優格冰淇淋中嗜熱鏈球菌菌數相對於在-20℃環境中的儲存天數的變化曲線圖。 圖6為本發明實施例的優格冰淇淋中乳酸菌菌數相對於山刺番荔枝果肉含量的變化的分析結果圖。 圖7為本發明實施例的優格冰淇淋中嗜熱鏈球菌菌數相對於山刺番荔枝果肉含量的變化的分析結果圖。 圖8為為本發明實施例的優格冰淇淋的DPPH自由基清除率相對於山刺番荔枝果肉含量的變化的分析結果圖。 圖9為本發明實施例的優格冰淇淋的總多酚含量相對於山刺番荔枝果肉含量的變化的分析結果圖。 圖10為本發明實施例的優格冰淇淋的感官品評的分析結果圖。FIG. 1 is a flow block diagram of a method for manufacturing a yogurt ice cream according to an embodiment of the present invention. FIG. 2 is a graph showing analysis results of gastric acid tolerance of lactic acid bacteria in Yogurt ice cream according to an embodiment of the present invention. FIG. 3 is a graph showing analysis results of bile salt tolerance of lactic acid bacteria in Yogurt ice cream according to an embodiment of the present invention. FIG. 4 is a graph showing a change curve of the number of lactic acid bacteria in the yogurt ice cream according to the embodiment of the present invention with respect to the number of days of storage in an environment of -20 ° C. FIG. 5 is a graph showing a change curve of the number of Streptococcus thermophilus relative to the number of days of storage in an environment of -20 ° C. according to an embodiment of the present invention. FIG. 6 is a graph of analysis results of changes in the number of lactic acid bacteria in Yogurt ice cream relative to the content of Soursop fruit in the example of the present invention. FIG. 7 is a graph of analysis results of changes in the number of Streptococcus thermophilus relative to the content of Soursop fruit in Yogurt ice cream according to an example of the present invention. FIG. 8 is a graph showing analysis results of changes in DPPH radical scavenging rate of Yogurt ice cream with respect to the content of Soursop fruit in the Example of the present invention. FIG. 9 is a graph showing the analysis result of the change of the total polyphenol content of the Yogurt ice cream with respect to the content of the fruit of Soursop. FIG. 10 is a diagram of analysis results of sensory evaluation of yogurt ice cream according to an embodiment of the present invention.

Claims (5)

一種優格冰淇淋,包含:10重量%至25重量%的牛奶;5重量%至20重量%的山刺番荔枝(Annona muricata)果肉;27重量%的優格,其中該優格包含9.59~9.67 log CFU/毫升的保加利亞乳酸桿菌及9.70~9.82 log CFU/毫升的嗜熱鏈球菌;12重量%的甜味料;13重量%的蛋;及18重量%的奶油。A Yogurt ice cream, comprising: 10% to 25% by weight of milk; 5% to 20% by weight of Annona muricata pulp; 27% by weight of Yogurt, wherein the Yogurt contains 9.59 to 9.67 Log CFU / ml of Lactobacillus bulgaricus and 9.70 ~ 9.82 log CFU / ml of Streptococcus thermophilus; 12% by weight of sweeteners; 13% by weight of eggs; and 18% by weight of cream. 如請求項1所述之優格冰淇淋,其中該山刺番荔枝的含量為5重量%至10重量%。The yogurt ice cream according to claim 1, wherein the content of the Soursop is 5 to 10% by weight. 如請求項2所述之優格冰淇淋,其中該山刺番荔枝的含量為5重量%。The yogurt ice cream according to claim 2, wherein the content of the Soursop is 5 wt%. 一種優格冰淇淋的製作方法,包括:將蛋與甜味料混合成一第一混合物;將牛奶及奶油加入至該第一混合物中以混合成一第二混合物;加熱該第二混合物;以調理棒攪拌加熱後的該第二混合物3分鐘以形成一均質物;將該均質物於4℃熟成16小時;將山刺番荔枝果肉及優格添加至經熟成的該均質物形成一冰淇淋混料,其中該冰淇淋混料具有10~25重量%的該牛奶、5~20重量%的該山刺番荔枝果肉、27重量%的該優格、12重量%的該甜味料、13重量%的該蛋黃液與18重量%的該奶油,其中該優格包含9.59~9.67 log CFU/毫升的保加利亞乳酸桿菌及9.70~9.82 log CFU/毫升的嗜熱鏈球菌;及以攪凍機攪凍至該冰淇淋混料呈現濃稠膏狀。A method for making yogurt ice cream, comprising: mixing eggs and sweeteners into a first mixture; adding milk and cream to the first mixture to mix into a second mixture; heating the second mixture; stirring with a conditioning rod The second mixture is heated for 3 minutes to form a homogeneous substance; the homogeneous substance is matured at 4 ° C for 16 hours; the succulent fruit and yogurt are added to the matured homogeneous substance to form an ice cream mixture, wherein The ice cream mix has 10-25% by weight of the milk, 5-20% by weight of the Soursop fruit pulp, 27% by weight of the Yogurt, 12% by weight of the sweetener, and 13% by weight of the egg yolk. And 18% by weight of the cream, wherein the yogurt contains 9.59 to 9.67 log CFU / ml of Lactobacillus bulgaricus and 9.70 to 9.82 log CFU / ml of Streptococcus thermophilus; The material was thick and creamy. 如請求項4所述之優格冰淇淋的製作方法,更包括:於小於或等於-20℃下硬化經攪凍的該冰淇淋混料。The method for manufacturing a yogurt ice cream according to claim 4, further comprising: hardening the frozen ice cream mixture at a temperature of less than or equal to -20 ° C.
TW106136989A 2017-10-26 2017-10-26 Frozen yogurt and method for preparing thereof TWI671013B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
TW106136989A TWI671013B (en) 2017-10-26 2017-10-26 Frozen yogurt and method for preparing thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
TW106136989A TWI671013B (en) 2017-10-26 2017-10-26 Frozen yogurt and method for preparing thereof

Publications (2)

Publication Number Publication Date
TW201916805A TW201916805A (en) 2019-05-01
TWI671013B true TWI671013B (en) 2019-09-11

Family

ID=67347441

Family Applications (1)

Application Number Title Priority Date Filing Date
TW106136989A TWI671013B (en) 2017-10-26 2017-10-26 Frozen yogurt and method for preparing thereof

Country Status (1)

Country Link
TW (1) TWI671013B (en)

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
山刺番荔枝,2014年4月28日,http://kplant.biodiv.tw/%E5%B1%B1%E5%88%BA%E7%95%AA%E8%8D%94%E6%9E%9D/%E5%B1%B1%E5%88%BA%E7%95%AA%E8%8D%94%E6%9E%9D.htm *
山刺番荔枝,2014年4月28日,http://kplant.biodiv.tw/%E5%B1%B1%E5%88%BA%E7%95%AA%E8%8D%94%E6%9E%9D/%E5%B1%B1%E5%88%BA%E7%95%AA%E8%8D%94%E6%9E%9D.htm。
閆慶標,酸奶冰淇淋生產技術的探討,乳品工業,1994年第2期 *
閆慶標,酸奶冰淇淋生產技術的探討,乳品工業,1994年第2期。

Also Published As

Publication number Publication date
TW201916805A (en) 2019-05-01

Similar Documents

Publication Publication Date Title
Soukoulis et al. Ice cream as a vehicle for incorporating health‐promoting ingredients: Conceptualization and overview of quality and storage stability
CN111601508B (en) Non-dairy fermented food
JP4862076B2 (en) Fermented food
CN105053187B (en) A kind of coconut taste acidified milk and preparation method thereof
RU2468591C1 (en) Whey beverage production method
CN114052232A (en) Cream
US20090035288A1 (en) Edible product containing beneficial bacteria
KR101597260B1 (en) Composite of powder youhurt and intake method
CN107897494A (en) A kind of preparation method of compound probiotic ice cream
CN104363765B (en) Food product with lactic cultures largely living
US20110212224A1 (en) Method for obtaining a mixture of probiotics, prebiotics nutrients with synergistic symbiotic action
CN107744047A (en) A kind of no sucrose fermented type ice cream or the preparation method of ice cream and its slurry
Low et al. Effects of different levels of refined cane sugar and unrefined coconut palm sugar on the survivability of Lactobacillus acidophilus in probiotic ice cream and its sensory and antioxidant properties
CN102067913B (en) Partly skimmed cheese and preparation method thereof
RU2532047C1 (en) Ice-cream production method
JP2008167747A (en) Lactic acid-fermented food composition of cacao mass and method for producing the same
CN108094663A (en) A kind of compound probiotic dried ice cream mix
KR20110077180A (en) Apple pudding composition with good organoleptic properties and its preparation method
TWI671013B (en) Frozen yogurt and method for preparing thereof
TW201717784A (en) Lactic acid bacterium-containing fat or oil composition and method for manufacturing same
Khaji et al. The investigation of probiotics viability, physicochemical and rheological properties of a probiotic dessert based on sesame paste (Tahini)
CN108142637A (en) A kind of preparation method of probiotic ice cream
Shahsavan et al. Effect of different amounts of sugar and fat on the viability of Lactobacillus casei, physical, chemical and sensory properties of probiotic ice cream
RU2141766C1 (en) Cultured-milk product &#34;narine&#34; for production of dessert in frozen condition
WO2003028472A1 (en) Method of improving physical properties of pwdered green tea and powdered green tea preparations with improved physical properties