TWI666315B - An Taiwanofungus camphoratus culture medium for increasing the fruiting bodies and triterpenoids - Google Patents

Abstract

An antrodia cinnamomea culture medium component capable of increasing antrodia cinnamomea fruit bodies and improving triterpenes, comprising agar, D-glucose, malt extract, and 0.5 to 1% peptone. Therefore, adding peptone to Antrodia cinnamomea medium can effectively improve the quality of Antrodia cinnamomea.

Description

Antrodia cinnamomea fruit body and triterpenoids

The present invention relates to the cultivation of medicinal fungi; in particular, it relates to a composition of antrodia cinnamomea that can be used to increase the body mass of antrodia cinnamomea and increase triterpenoids.

Antrodia cinnamomea, also known as Antrodia cinnamomea, is one of the six major medicinal fungi unique to Taiwan. It only grows on the decayed inner wall of the anemone tree's trunk that is unique to Taiwan for more than 100 years, or on the damp surface of dead and dead anemone tree wood. The wild wild camphor tree has been listed by the Taiwanese government as one of the conservation species in recent years, and export is not allowed. Currently only in Taiwan, it is extremely precious and is regarded as the treasure of Taiwan, the ruby in the forest, and the king of ganoderma.

The medicinal benefits of Antrodia cinnamomea include:

1. Detoxification and detoxification;

Second, resist virus damage, prevent influenza, improve immunity, because the polysaccharide contained in Antrodia cinnamomea ingredients can improve the immune regulation in the human body.

3. Prevent cancer and inhibit the growth of cancer cells;

Fourth, the treatment of hepatitis, cirrhosis, liver cancer, because Antrodia cinnamomea rich in triterpenoids and polysaccharides, has the effect of protecting the liver, promoting liver cell regeneration, and fighting liver cancer.

V. Treatment of various cardiovascular and cerebrovascular diseases. Among them, Antrodia cinnamomea can reduce blood cholesterol and fat content, and its adenosine can reduce platelet coagulation function, so it can treat various cardiovascular and cerebrovascular diseases such as hypertension, hypotension, Arteriosclerosis and more.

With the improvement of people's awareness of Antrodia cinnamomea and its medicinal effects, its demand for health care is increasing. However, the existing cultivation methods: segment wood cultivation method, individual cultivation method and liquid fermentation method can not produce high quality, mass production and Short cycle of Antrodia. That is, there is still a lack of a cultivation method capable of cultivating high quality and short cycle Antrodia cinnamomea.

In view of this, an object of the present invention is to provide a culture medium of Antrodia cinnamomea which can increase the fruiting body of Antrodia cinnamomea and raise triterpenes, and establish an optimum medium and an index of various components of Antrodia cinnamomea.

In order to achieve the above-mentioned object, the present invention provides a culture medium of Antrodia cinnamomea that can increase the fruit body of Antrodia cinnamomea and raise triterpenes, which comprises agar, D-glucose, malt extract and 0.5 to 1% peptone.

The effect of the present invention is that the plant protein peptone is added to the Antrodia cinnamomea medium, which effectively improves the quality of Antrodia cinnamomea.

Figure 1 shows the high performance liquid chromatography (HPLC) analysis of medium A0.5 and medium P0.5.

Figure 2 shows the effect of Antrodia cinnamomea on ALT and AST in mice with acute alcoholic liver injury.

Figure 3 shows the effects of Antrodia cinnamomea on the levels of MDA, GSH-Px and SOD in mice with acute alcoholic liver injury.

In order to explain the present invention more clearly, the following preferred embodiments are described in detail below.

Example 1 Exploring the Optimum Culture Conditions of Antrodia cinnamomea and Its Component Analysis

Source of bacteria

The fungus and plant biotechnology team at the University of Science and Technology of China collected fresh Antrodia cinnamomea from the mountain and isolated it.

Explore the best culture conditions

MEA medium is mainly used for the isolation, counting and cultivation of fungi (molds, yeasts, etc.), and the medium of the present invention contains the basic ingredients: grains (corn, rice or wheat, etc.), which contains 10-15% purple rice Therefore, the color is reddish, and 0.1-1% peptone is added. Among them, peptone slows down the growth rate, so it can grow similar fruit body structure.

Antrodia cinnamomea fruit body culture device mainly includes: a bag body, which is composed of a ring wall and a top wall, so that an opening is formed on the top side and an accommodation space is formed inside. The aforementioned culture medium is contained in the accommodating space of the bag body, thereby cultivating Antrodia cinnamomea. The use of this cultivation method can greatly increase production and reduce costs.

The experiment of the present invention is mainly based on solid medium and cultured under the conditions of five mediums, which are respectively represented by the codes MEA, P0.1, P0.5, A0.1, A0.5, and the control group is MEA Culture medium, experimental group was added 0.1% and 0.5% of animal and plant peptone, as shown in Table 1.

The configuration method in the table above is: adding agar, D-glucose, malt extract and peptone to distilled water to prepare a 1 liter solution, sterilizing at 121 ° C for 15 minutes, and adjusting the pH to 5.6 ± 0.2. Among them, the preferred weight percentage concentrations of agar, D-glucose and malt extract are 2%, 4% and 2%, respectively.

Comparison of diameter growth and dry weight

When inoculating Antrodia camphorii with a drill, make sure that the diameter of each inoculated strain is the same. Then pick a piece of bacteria and put it in the center of the culture medium. Then put it in a 26 ° C incubator for 1 or 2 cultures. After measuring and recording the mycelial growth diameter, it was dried in an oven for 46 hours. After drying, the portion of the culture medium without Antrodia camphorata hypha was cut off with scissors, and the weight was recorded. The results are shown in Table II below.

The groups with the most significant increase in diameter and dry weight, P0.5 and A0.5 (after 2 months of culture), were analyzed by high performance liquid chromatography (HPLC). The results are shown in Figure 1. The marked A, B peak at P0.5 exists alone and the C peak is higher. Therefore, the optimum solid culture conditions of Antrodia cinnamomea were determined to be the components contained in the P0.5 group.

Index component analysis

The medium (P0.5) of the present invention is characterized by adding 0.5-1% plant peptone, and its index component content is shown in Table 3 below.

Example 2 Antrodia cinnamomea repairing liver damage

Alcohol-induced acute alcoholic liver injury in mice

After one week of acclimation, C57BL / 6 mice were randomly divided into three groups of six. The grouping method is shown in Table 4. Table 4. Groups of Antrodia cinnamomea for repairing liver damage

Among them, physiological saline and Antrodia cinnamomea powder were administered at 9 am every day. After continuous gastric administration for 30 days, the mice were fasted for 6 hours, and then administered with alcohol (5.0 g / kg, 60%). After 16 hours, the mice were sacrificed and blood was taken. The serum was used to determine various biochemical parameters. Another part of the mouse liver was added to prepare a liver tissue homogenate by adding a quantitative physiological saline solution, and the degree of liver damage was evaluated.

Effects of Antrodia cinnamomea on activity of marker enzymes in acute alcoholic liver injury

Glutamate aminotransferase (ALT) and aspartate aminotransferase (AST) are hallmark enzymes in the liver. When the liver is damaged, the two will produce abnormal elevation. This experiment investigated the effects of Antrodia cinnamomea powder on the content of alanine aminotransferase and aspartate aminotransferase in mice with acute alcoholic liver injury. The results are shown in Figure 2.

Compared with the control group, the levels of alanine aminotransferase and aspartate aminotransferase in the model group were significantly higher. Antrodia cinnamomea powder can significantly inhibit the increase of alanine aminotransferase and aspartate aminotransferase in mice with acute alcoholic liver injury. Among them, compared with the blank group, *** p <0.001; compared with the model group, # # #p <0.001, # #p <0.01.

Effects of Antrodia cinnamomea on the antioxidant activity of acute alcoholic liver injury

MDA is a secondary product produced by lipid peroxidation. SOD and GSH-Px are the key enzymes for the liver to remove peroxides and prevent oxidative damage. The content of liver MDA and the activity of SOD and GSH-Px enzymes can reflect lipid peroxidation in the liver. And the extent of oxidative damage. This experiment investigates The effects of MDA content and SOD and GSH-Px enzyme activity in the liver of mice with alcoholic liver injury are shown in Figure 3.

Compared with the control group, the MDA content in the model group was significantly increased, while the SOD and GSH-Px activities were significantly reduced. Antrodia cinnamomea powder can significantly inhibit the increase of MDA content and the decrease of SOD and GSH-Px activity in mice with acute alcoholic liver injury. Among them, compared with the blank group, *** p <0.001; compared with the model group, # # p <0.01.

The above experiments show that Antrodia cinnamomea powder has a certain protective effect on acute alcoholic liver in mice.

In summary, using the ingredients of the Antrodia cinnamomea medium of the present invention can indeed produce high-quality and short-period Antrodia cinnamomea.

The above descriptions are only the preferred and feasible embodiments of the present invention, and any equivalent changes made by applying the description of the present invention and the scope of patent application should be included in the patent scope of the present invention.

Claims (6)

Antrodia cinnamomea culture medium capable of increasing fruit body of antrodia cinnamomea and raising triterpenes, comprising agar, D-glucose, malt extract and 0.5 to 1% peptone, wherein the peptone is vegetable peptone, and the plant peptone is soy peptone . Antrodia cinnamomea culture medium, which can increase the fruiting body of antrodia cinnamomea and triterpenoids as described in claim 1, has a pH range of 5.6 ± 0.2. Antrodia cinnamomea culture medium, which can increase the fruiting body of antrodia cinnamomea and triterpenoids, as described in claim 1, including grains. Antrodia cinnamomea lucidum culture medium capable of increasing antrodia cinnamomea fruit bodies and enhancing triterpenoids as described in claim 3, wherein the grain cereal contains 10-15% purple rice. Antrodia cinnamomea culture medium that can increase antrodia cinnamomea fruit bodies and enhance triterpenes as described in claim 1, wherein the weight percentage concentrations of the agar, D-glucose and malt extract are 1.5-2%, 3-5% and 2-3%. Antrodia cinnamomea culture medium that can increase antrodia cinnamomea fruit bodies and enhance triterpenes as described in claim 5, wherein the weight percentage concentrations of the agar, D-glucose and malt extract are 2%, 4% and 2%, respectively.