TWI656218B - Method and device for generating adaptive cell culture parameters and storage medium thereof - Google Patents

Abstract

A method for producing adaptive cell culture parameters for culturing a first cell as a target cell, the method comprising: searching for a generalized cell culture from a database according to a first cell type and a target cell characteristic of the first cell; a parameter to establish a basic control factor sequence table; generating adaptive cell culture parameters according to the first cell type, the target cell characteristics, and the basic control factor sequence table; and cultivating the first cell according to the adaptive cell culture parameter, and A culture result is produced; and the above adaptive cell culture parameters are adjusted according to the above culture results.

Description

Method, device and storage for adaptive cell culture parameter generation media

The present disclosure relates to a cell engineering technique pertaining to the field of bioengineering technology, and more particularly to a method, apparatus and storage medium for the production of adaptive cell culture parameters.

The process of cell culture is affected by many variables, such as environmental control parameters (cavity temperature, incubator CO2 or hypoxic concentration, humidity, ...), cell culture control parameters (pH concentration, medium and growth factor addition type and amount) Parameters such as replacement time, cell culture time, trypsin treatment time, contamination detection results, cell inoculation number and density, cell growth number not as expected, ...).

Different types of cell culture have different control parameters. When the parameters are not properly controlled, the cell characteristics of the cultured cells will be inconsistent with the expected or cell death. In addition, changes in environmental factors around the environment can also affect the results of cell growth. Since the conditions in which the cells are taken from the mother are different and the external culture environment is changed, the same culture parameters may occur but cannot be used.

Therefore, it is necessary to provide a method, a device and a storage medium for the production of adaptive cell culture parameters, which can avoid cells in addition to the culture parameters. Death, improving the characteristics of cell growth in line with expectations.

The disclosures below are merely exemplary and are not meant to be limiting in any way. Other aspects, embodiments, and features will be apparent from the description and drawings. That is, the following disclosure is provided to introduce concepts, advantages, benefits, and novel and non-obvious technical advantages described herein. Selected, not all, embodiments will be described in further detail below. Therefore, the following disclosure is not intended to be an essential feature of the claimed subject matter, and is not intended to be used in the scope of the claimed subject matter.

The present disclosure provides a method, apparatus, and storage medium for the production of adaptive cell culture parameters.

The present disclosure proposes a method for generating adaptive cell culture parameters for culturing a first cell as a target cell, the method comprising: searching for a general database according to a first cell type and a target cell characteristic of the first cell; The cell culture parameters are used to establish a basic control factor sequence table; the adaptive cell culture parameters are generated according to the first cell type, the target cell characteristics, and the basic control factor sequence table; and the first cell is cultured according to the above adaptive cell culture parameters. The cells are produced and a culture result is produced; and the above adaptive cell culture parameters are adjusted according to the above culture results.

The present disclosure further provides a device for generating adaptive cell culture parameters for culturing a first cell as a target cell, the device comprising: a control circuit; a processor mounted to the control circuit; and a memory mounted to The above control circuit is coupled to the processor; wherein the processor is configured to execute Storing a code in the memory to perform: searching a generalized cell culture parameter from a database according to the first cell type and a target cell characteristic of the first cell to establish a basic control factor sequence table; Obtaining adaptive cell culture parameters according to the first cell type, the target cell characteristics, and the basic control factor sequence table; cultivating the first cell according to the adaptive cell culture parameter, and generating a culture result; and adjusting according to the culture result The above adaptive cell culture parameters.

The disclosure further provides a storage medium for reading a code stored in the storage medium via a device to perform an adaptive cell culture parameter generation method for cultivating the first cell as a target cell, wherein the method comprises the following steps: Generating a generalized cell culture parameter from a database according to the first cell type and a target cell characteristic of the first cell, to establish a basic control factor sequence table; according to the first cell type, the target cell characteristics, and the above The basic control factor sequence table generates adaptive cell culture parameters; the first cells are cultured according to the above adaptive cell culture parameters, and a culture result is produced; and the above adaptive cell culture parameters are adjusted according to the above culture results.

100‧‧‧ cell culture system

110‧‧‧Management device

120‧‧‧ incubator

122‧‧‧ Petri dishes

124‧‧‧ cell culture medium

126‧‧‧Cultivated cells

130‧‧‧Storage device

200‧‧‧Electronic devices

202‧‧‧ Input device

204‧‧‧Output device

206‧‧‧Control circuit

208‧‧‧Central Processing Unit

210‧‧‧ memory

212‧‧‧Executing code

214‧‧‧ transceiver

300‧‧‧ method

S305, S310, S315, S320‧‧‧ steps

400‧‧‧ method

Steps S405, S410, S415‧‧

600‧‧‧ method

S605, S610, S615, S620‧‧‧ steps

900‧‧‧ method

S905, S910, S915, S920‧‧‧ steps

1200‧‧‧ method

S1205, S1210, S1215, S1220, S1225‧‧‧ steps

The figures are included to provide a further understanding of the disclosure and are incorporated in and constitute a part of the disclosure. The drawings illustrate the embodiments of the disclosure and, together with the description, illustrate the principles of the disclosure. It is understood that the drawings are not necessarily to scale,

1 is an exemplary schematic diagram showing a cell culture system for producing adaptive cell culture parameters in accordance with an embodiment of the present disclosure.

FIG. 2 is a simplified functional block diagram of an electronic device according to an embodiment of the present disclosure.

Figure 3 is a flow chart showing a method of generating adaptive cell culture parameters in accordance with an embodiment of the present disclosure.

Figure 4 is a flow chart showing a method of establishing a basic control factor sequence table in accordance with an embodiment of the present disclosure.

Figure 5 is a table showing the basic control factor sequence of the first cell of the first cell type A1 according to an embodiment of the present disclosure.

Figure 6 is a flow chart showing a method of generating adaptive cell culture parameters in accordance with an embodiment of the present disclosure.

Figure 7 is a table showing the basic control factor sequence of known cells (cell type A1) according to an embodiment of the present disclosure.

Fig. 8 is a table showing the basic control factor sequence of the third cell (cell type A11) cultured as a target cell (cell type B11) according to an embodiment of the present disclosure.

Figure 9 is a flow chart showing a method of adjusting adaptive cell culture parameters based on culture results in accordance with an embodiment of the present disclosure.

Figure 10 is a table showing the basic control factor sequence of the first cell in accordance with an embodiment of the present disclosure.

Figure 11 is a table showing the basic control factor sequence of the first cell being cultured into a second cell in accordance with an embodiment of the present disclosure.

Figure 12 is a diagram showing the execution of a pH on a first cell in accordance with an embodiment of the present disclosure. Flow chart of the method of concentration prediction mechanism.

Various aspects of the disclosure will be more fully described below with reference to the drawings. However, the disclosure may be embodied in many different forms and should not be construed as being limited to any particular structure or function presented. Rather, these aspects are provided so that this disclosure will be thorough and complete, and the disclosure will fully convey the scope of the disclosure. Based on the teachings herein, those skilled in the art will appreciate that the scope of the present disclosure is intended to cover any aspect disclosed herein, either alone or in combination with any other aspect disclosed herein. For example, any number of devices or methods of implementation presented herein can be used. In addition, the scope of the present disclosure is intended to cover an apparatus or method that is implemented using other structures, functions, or structures and functions, in addition to the various aspects disclosed herein. It should be understood that any aspect disclosed herein may be embodied by one or more elements of the patent application.

The word "exemplary" is used herein to mean "serving as an example, instance or description." Any aspect of the disclosure or a design described herein as "exemplary" is not necessarily to be construed as preferred or preferred. In addition, the same numerals indicate the same elements in all the figures, and the articles "a" and "an"

1 is a schematic diagram showing a cell culture system 100 in which adaptive cell culture parameters are generated in accordance with an embodiment of the present disclosure.

The cell culture system 100 can include at least one incubator 120 and a management device 110, wherein the management device 110 can be connected to the incubator 120 by wire or wireless transmission. Wireless transmission is like regional network, internet, Bluetooth (Bluetooth), Wi-Fi, Wi-Fi Direct, Zigbee and other transmission methods.

In one embodiment, the management device 110 can be connected to a storage device 130 via a network, wherein the network can be any type of network familiar to those skilled in the art that can use any of a variety of communication-available protocols. To support data communications, including but not limited to TCP/IP and more. For example, the network can be a local area network (LAN), such as Ethernet, etc., a virtual network, including but not limited to the Internet, wireless network And/or any combination of these and/or other networks. The storage device 130 can store a database for storing all generalized cell culture parameters as well as cell related information.

One or more culture dishes 122 (or other forms of culture vessels) may be contained in the incubator 120. The incubator 120 itself is conventional and the environment within the incubator 120 can be controlled by the management device 110. The management device 110 can be a cloud device, a personal computer, a notebook computer, a mobile device, etc., which can be used to control the temperature, humidity, pH concentration, carbon dioxide, oxygen, and other gas components in the incubator 120.

Petri dishes 122 are widely used commercially in a variety of standard sizes, typically made of clear plastic (sometimes glass). The culture dish 122 is typically filled with cell culture medium 124. The cell culture medium 124 can, for example, comprise a variety of nutrient gels in which the amount and composition of nutrients are adapted to the particular type of cells 126 that will be cultured in the culture vessel. Those skilled in the art will appreciate that the culture dish 122 and cell culture medium 124 are not limited to the disclosure.

In addition, in the cell culture system 100 of the disclosed embodiment, one or more sensing devices (not shown) may be further included, which may be installed in each of the culture dishes 122 to detect the state of the cells or the surroundings thereof. The state of the environment. In an embodiment, the sensing device can include a plurality of sensors for monitoring parameters related to cell growth, including Differentiation parameters (eg, protein) sensors, environmental parameters (eg, temperature) sensors, and monitoring parameters (eg, pH concentration) sensors are induced. The sensing device may also include other sensors associated with the growth of specific cells being propagated, including, for example, sodium and/or potassium ion sensors. The sensing device can also have a sensor for measuring gaseous environmental parameters (particularly carbon dioxide and/or oxygen) associated with or indicative of cell growth activity. A sensing device can be provided to measure humidity and other gas components in the culture dish. The sensing device may preferably be configured to make measurements periodically, periodically, or when the user initiates a request through the management device 110. The sensing device can wirelessly output the above measured value to the management device 110 after the measurement, for analysis by the management device 110.

FIG. 2 is a simplified functional block diagram of an electronic device 200 according to an embodiment of the present disclosure. As shown in FIG. 2, the electronic device 200 may be the management device 110 in the cell culture system 100 of Fig. 1. The electronic device 200 can include an input device 202, an output device 204, a control circuit 206, a central processing unit (CPU) 208, a memory 210, a code 212, and a transceiver 214. The control circuit 206 executes the program code 212 in the memory 210 through the central processing unit 208, and thereby controls the operations performed in the electronic device 200. The electronic device 200 can receive the user input signal by using the input device 202 (for example, a keyboard, a numeric keypad, a touch screen, or a microphone (sound input); and the output device 204 (such as a screen or a speaker) can also output images and sounds. The transceiver 214 is here used to receive and transmit wireless signals, send the received signals to the control circuit 206, and wirelessly output the signals generated by the control circuit 206.

3 is a flow chart showing a method 300 of adaptive cell culture parameter generation in accordance with an embodiment of the present disclosure. This method can be applied to the management device 110 in the cell culture system 100 of Fig. 1 to culture the first cell as a target cell.

In step S305, the management device searches for a generalized cell culture parameter from a database according to the first cell type and a target cell characteristic of the first cell, to establish a basic control factor sequence table, wherein the researcher can pre-inform the data. All generalized cell culture parameters are stored in the library for management device search. Next, in step S310, the management device generates adaptive cell culture parameters based on the first cell type, the target cell characteristics, and the basic control factor sequence table. Further, in step S315, the management device cultures the first cells according to the adaptive cell culture parameters and produces a culture result. In step S320, the management device adjusts the adaptive cell culture parameters based on the culture results.

An exemplary operational flow for managing how the device establishes a basic control factor sequence table and how to generate and adjust adaptive cell culture parameters will be described in detail below.

4 is a flow chart showing a method 400 of establishing a basic control factor sequence listing in accordance with an embodiment of the present disclosure. This method 400 can be performed by the management device 110 in the cell culture system 100 of FIG. 1 to establish a basic control factor sequence listing for the first cell.

Before the start of the process, the management device may first confirm the first cell species of the first cell and the target cell characteristics of the target cell to be cultured. In step S405, the management device determines whether the first cell is a known cell based on the first cell type. In this step, the management device can align the first cell type with the cell type of all cells in the database. When the management device has aligned the same cells as the first cell species, it is determined that the first cell line is a known cell.

Next, when the management device determines that the first cell is a known cell (YES in step S405), in step S410, the management device is obtained from the database. Corresponding to the basic control factors of the known cells, the weights and priority orders corresponding to the basic control factors are set, and the basic control factors, the weights of the corresponding basic control factors, and the priority order are stored in the basic control factor sequence table of the first cell.

When the management device determines that the first cell is not a known cell (NO in step S405), in step S415, the management device stores the weight and priority order of the preset basic control factor and the corresponding preset basic control factor. In the basic control factor sequence table of the first cell. It should be noted that the weight and priority order of the preset basic control factor and the corresponding preset basic control factor may be pre-stored in the database by the user.

In addition, in another embodiment, after the management device establishes the basic control factor sequence table of the first cell, the data in the database can be continuously searched to update the basic control factor sequence table. For example, Figure 5 is a table showing the basic control factor sequence for the first cell of the first cell type A1. As shown in the figure, the background white portion is the basic control factor sequence table established by the management device, and the background gray portion is updated after the management device searches the database again to establish new control items and new control factors. The control items for each control factor can be given basic weights by the researcher in advance. The higher the weight given, the meaning that the control of this control factor is closer to the steady growth of the cells. The upper part of Fig. 5 is the corresponding weight of the first cell under different control factors (inducing differentiation parameters, environmental control parameters, monitoring parameters). The lower half of Figure 5 is the priority corresponding to each control factor, and the priority can also be given in advance by the researcher. The smaller the priority value, the higher the priority of the priority adjustment of the control factor (ie, the least priority order). As shown in Fig. 5, since the priority value corresponding to the induced differentiation parameter is 0 (i.e., the priority of the differentiation parameter is the highest), the weight of the induced differentiation parameter will be preferentially adjusted.

Figure 6 is a flow diagram showing a method 600 of generating adaptive cell culture parameters in accordance with an embodiment of the present disclosure. This method can be performed by the management device 110 in the cell culture system 100 of Figure 1, for generating adaptive cell culture parameters based on the first cell type of the first cell, the target cell characteristics, and the basic control factor sequence listing.

Before the start of the process, the management device may first confirm the first cell species of the first cell and the target cell characteristics of the target cell to be cultured. In step S605, the management device determines whether the first cell is a known cell based on the first cell type of the first cell. In this step, the management device can align the first cell type with the cell type of all cells in the database. When the management device has aligned the same cells as the first cell species, it is determined that the first cell line is a known cell.

When the management device determines that the first cell is a known cell (YES in step S605), in step S610, the management device acquires a basic control factor corresponding to the known cell from the database. In step S615, the management device selects the basic control factor having the highest weight from each of the basic control factors as the adaptive cell culture parameter. For example, please refer to Figure 7, assuming that Figure 7 is a basic control factor sequence table of known cells (cell type A1), the management device will select the parameter with the highest weight among the basic control factors as the adaptability. Cell culture parameters. When the weights are the same, the management device selects the higher ranked parameters in the table as the adaptive cell culture parameters. As shown in Fig. 7, in the table in which the basic control factor is the induced differentiation parameter, since all the differentiation parameter parameters have a weight of 255, the management device can select the higher-order induced differentiation parameter v1 in the table as the adaptive cell culture. parameter. In a table in which the basic control factor is the environmental control parameter, the management device can select the environmental control parameter v3 having the highest weight 257 as the adaptive cell culture parameter. In the table where the basic control factor is the monitoring parameter, the management device can select the supervisor with the highest weight 257 The parameter v3 was controlled as an adaptive cell culture parameter.

When the management device determines that the first cell is not a known cell (NO in step S605), in step S620, the management device acquires a third cell similar to the first cell type of the first cell from the database. The basic control factor corresponding to the third cell is replicated as a basic control factor corresponding to the first cell. Next, in step S615, the management device selects the basic control factor having the highest weight from each of the basic control factors as the adaptive cell culture parameter. For example, please refer to Figure 8. If the figure 8 is the basic control factor sequence table for the third cell (cell type A11) cultured as the target cell (cell type B11), the management device directly copies the third. The basic control factor sequence table of the cells is used as the basic control factor sequence table corresponding to the first cell, and the parameter with the highest weight is selected from the basic control factors as the adaptive cell culture parameter.

Figure 9 is a flow diagram showing a method 900 for adjusting adaptive cell culture parameters based on culture results in accordance with an embodiment of the present disclosure. This method can be performed by the management device 110 in the cell culture system 100 of Fig. 1 to adjust the adaptive cell culture parameters based on the culture results, wherein the culture results are that the first cells are cultured as the second cells.

The management device can confirm the cell culture results before the process begins. In step S905, the management device determines whether the second cell type of one of the second cells is the same as the target cell type. When the second cell type of one of the second cells is the same as the target cell type (YES in step S905), in step S910, the management device determines whether the second cell characteristic of one of the second cells is different from the target cell characteristic Compatible.

When the second cell characteristic of the second cell matches the target cell characteristic ("Yes" in step S910), the management device determines that the second cell is the target The cells do not need to adjust the adaptive cell culture parameters to end the process.

When the second cell characteristic of the second cell does not match the target cell characteristic (NO in step S910), in step S915, the management device adjusts the weight of the basic control factor in the basic control factor sequence table of the first cell. And the priority order, as the basic control factor with the highest weight is reselected as the above adaptive cell culture parameter when the same cell is cultured in the next stage. For example, FIG. 10 shows a basic control factor sequence table of a first cell according to an embodiment of the present disclosure, and refers to FIG. Assume that Figure 7 is a list of basic control factor sequences for the first cell before unadjustment. The management device lowers the weight of the basic control factor having the highest priority when determining that the second cell characteristic of the second cell does not match the target cell characteristic. In Figure 7, the basic control factor for the highest priority is the induced differentiation parameter. The induced differentiation parameter selected as the adaptive cell culture parameter was the induced differentiation parameter v1, so the weight of the induced differentiation parameter v1 was lowered from 255 to 254, as shown in FIG. In addition, the management device also lowers the priority value of this basic control factor. As shown in Figure 10, the priority value of the induced differentiation parameter was adjusted from 0 to 1. When the priorities are the same, the management device determines the ordering of the priorities in the order in the table. Therefore, the order of priority for inducing differentiation parameters, environmental control parameters, and monitoring parameters becomes 2, 1, and 3. At the next adjustment, the management device selects the environmental control parameters with priority order 1 as the adaptive cell culture parameters according to the priority order.

When the second cell type of the second cell is different from the target cell type (NO in step S905), in step S920, the management device copies the basic control factor sequence table as the basic control factor sequence of the second cell. List and store the basic control factor sequence table of the second cell into the database for the next management device to culture the second cell according to the basic control factor sequence table of the second cell. Figure 11 A basic control factor sequence listing in which a first cell is cultured into a second cell in accordance with an embodiment of the present disclosure is shown. As shown, the first cell has been cultured into a second cell of cell type B2. Therefore, the management device can store the basic control factor sequence table in FIG. 11 to the database. When the next time the user wants to train the first cell as the second cell, the basic control factor sequence table can be obtained from the database through the management device to culture the second cell.

In short, the adjustment rules for weights and priorities can be represented by Table 1 below.

For example, the management device aligns the second cells and target cells cultured by the first cells. When the second cell and the target cell are different cells, the management device can replicate the basic control factor to establish new data for culturing the second cell. When the second cell is the same cell as the target cell and the characteristics are consistent, the management device can maintain the current weight and priority. In another embodiment, the management device may weight the weight and priority to indicate that the closer the parameter is to the steady growth of the cell. When the second cell and the target cell are the same cell and the characteristics do not match, the management device can reduce the weight value of the corresponding parameter to The lowest, and the priority of the corresponding parameter is reduced by first order, so as to avoid this parameter being used again for reference in a short time, as shown in Fig. 10. When the first cell dies during the culture process, the management device can minimize the weight value of the corresponding parameter and lower the priority of the corresponding parameter by one step to reduce the probability of selecting the parameter as an adaptive cell culture parameter.

Figure 12 is a flow diagram showing a method 1200 of performing a pH concentration prediction mechanism for a first cell in accordance with an embodiment of the present disclosure. This method can be performed by the management device 110 in the cell culture system 100 of FIG. This mechanism is performed when the first cell is cultured.

In step S1205, the management device may calculate a pH predicted value according to the first cell type of the first cell, wherein the pH predictor ( pH _predict ) may be expressed as follows: pH _predict = pH _base - Coef × T ²

The pH _{base is} expressed as the initial pH concentration of the medium, the Coef system is a coefficient, and the T system is a time value in hours (hr).

Next, in step S1210, the management device determines whether the actual pH value of one of the first cells detected by the sensing device is less than a threshold. In an embodiment, the threshold can be preset by the user. When the actual pH value of the first cell is less than the threshold (YES in step S1210), in step S1215, the management device adjusts the basic control factor in the basic control factor sequence table of the first cell.

When the actual pH value of the first cell is not less than the threshold (NO in step S1210), in step S1220, the management device determines whether the actual pH value of the first cell is smaller than the pH predicted value. When the actual pH value of the first cell is less than the pH predicted value (YES in step S1220), the management device may perform more in step S1225. Change the culture medium of the first cell to avoid cell death. In another embodiment, in step S1225, the management device can adjust the frequency of the pH concentration return (eg, increase the frequency of the pH concentration return). In addition, the management device can count the number of times the actual pH value is less than the predicted pH value. When the number of times is accumulated to a predetermined value or more, the management device starts the replacement of the culture medium of the first cell to avoid cell death.

When the actual pH value of the first cell is not less than the pH predicted value (NO in step S1220), the process returns to step S1220, and the management device continuously detects whether the actual pH value of the first cell is smaller than the pH predicted value.

In addition, central processor 208 can execute program code 212 to perform the acts and steps described in the above-described embodiments, or otherwise described in the specification.

As described above, the method, the device and the storage medium for the adaptive cell culture parameter production proposed by the present disclosure can dynamically generate adaptive cell culture process parameters, and perform pH concentration on the cell culture medium during the process of culturing the cells. It is predicted that the problem of cell culture death will be reduced to improve the characteristics of cell growth in line with expectations.

The above embodiments are described using a variety of angles. It will be apparent that the teachings herein may be presented in a variety of ways, and that any particular structure or function disclosed in the examples is merely representative. In light of the teachings herein, anyone skilled in the art will appreciate that the content presented herein can be independently rendered in various different types or in a variety of different forms.

Those skilled in the art will understand that messages and signals can be presented in a variety of different technologies and techniques. For example, all of the data, instructions, commands, messages, signals, bits, symbols, and wafers that may be referenced above may be volts, currents, electromagnetic waves, magnetic fields or magnetic particles, light fields or light particles, or any combination thereof. Presented.

Those skilled in the art will appreciate that various illustrative logic blocks, modules, processors, devices, circuits, and logic steps are described herein for use with the electronic hardware (eg, source coded or Digital implementation of other technical designs, analogy implementation, or a combination of both), various forms of programming or design codes linked to instructions (referred to as "software" or "software modules" for convenience in the text), or a combination of the two.

In addition, various illustrative logic blocks, modules, and circuits, and various aspects disclosed herein may be implemented in an integrated circuit (IC), an access terminal, an access point, or an integrated circuit. , access terminal, access point execution.

Any specific sequence or layering of the procedures disclosed herein is by way of example only. Based on design preferences, it must be understood that any specific order or hierarchy of steps in the program may be rearranged within the scope of the disclosure. The accompanying claims are intended to be illustrative of the embodiments of the invention

The method and algorithm steps disclosed in the specification of the present disclosure can be directly applied to a hardware and a software module or a combination of the two directly by executing a processor.

Therefore, the present disclosure may also provide a storage medium, which reads a code stored in the storage medium via an electronic device to perform an adaptive cell culture parameter generation method, wherein the method steps are as described above, and therefore no further description is provided. .

The use of ordinal numbers such as "first", "second", "third", etc., used to modify elements in the scope of the patent application does not imply any priority, prioritization, prioritization between elements, or method The order of the steps performed, and used only as an identifier to distinguish between different elements having the same name (with different ordinal numbers).

Although the disclosure has been described above by way of example, it is not intended to limit the scope of the present invention, and the scope of protection of the present invention can be made without departing from the spirit and scope of the disclosure. This is subject to the definition of the scope of the patent application.

Claims (12)

A method for producing adaptive cell culture parameters for culturing a first cell as a target cell, the method comprising the steps of: searching for a generalization according to a first cell type and a target cell characteristic of the first cell; a cell culture parameter to establish a basic control factor sequence table, wherein the basic control factor sequence form comprises at least a basic control factor, and a weight and a priority order corresponding to the basic control factor; according to the first cell type, the target cell Characterization and the above-mentioned basic control factor sequence table generate adaptive cell culture parameters; cultivating said first cells according to said adaptive cell culture parameters, and producing a culture result; and adjusting said adaptive cell culture parameters according to said culture results; wherein said culture The result is that the first cell is cultured as a second cell, and the step of adjusting the adaptive cell culture parameter according to the culture result further comprises: determining whether the second cell type of the second cell is the same as the target cell type and determining The second fine Whether the second cell characteristic is consistent with the characteristics of the target cell; when the second cell species is the same as the target cell species and the second cell characteristic is consistent with the target cell characteristic, the second cell is determined as Target cell Adjusting the weight of the basic control factor in the basic control factor sequence table and the priority order when the second cell type is the same as the target cell type and the second cell characteristic does not match the target cell characteristic Re-selecting the basic control factor with the highest weight as the above adaptive cell culture parameter; or copying the above basic control when the second cell species is different from the target cell species and the second cell characteristic does not match the target cell characteristics The factor sequence table serves as a basic control factor sequence table for culturing the above first cells into the above second cells, and stores the above basic control factor sequence table into the above database. The method for producing an adaptive cell culture parameter according to claim 1, wherein the generalized cell culture parameter is searched for from the above-mentioned database according to the first cell type of the first cell and the target cell characteristic. The step of establishing the basic control factor sequence table further includes: determining whether the first cell is a known cell according to the first cell type; and determining that the first cell is the known cell, obtaining the corresponding data from the database Knowing the basic control factor of the cell, setting a weight and a priority order corresponding to the basic control factor, and storing the basic control factor, the weight corresponding to the basic control factor, and the priority order in the basic control factor sequence table; or Predetermining the storage base when the first cell is not the above known cell The control factor and the weight and priority order corresponding to the preset basic control factor are in the above basic control factor sequence table. The method for producing an adaptive cell culture parameter according to claim 1, wherein the adaptive cell culture parameter is generated according to the first cell type of the first cell, the target cell characteristic, and the basic control factor sequence table. The step further includes: determining whether the first cell is a known cell according to the first cell type of the first cell; and determining that the first cell is the known cell, and obtaining the corresponding known from the database The basic control factor of the cell, wherein the basic control factor having the highest weight is selected as the adaptive cell culture parameter from each of the basic control factors; or when it is determined that the first cell is not the above known cell, obtained from the above database a third cell similar to the first cell species of the first cell, replicating a basic control factor corresponding to the third cell as a basic control factor corresponding to the first cell, and selecting the highest weight from each of the basic control factors The basic control factor serves as the above adaptive cell culture parameter. The method for producing an adaptive cell culture parameter according to claim 1, wherein the step of cultivating the first cell according to the adaptive cell culture parameter and generating the culture result further comprises: performing a first cell on the first cell. pH concentration prediction mechanism; wherein the above pH concentration prediction mechanism includes: Calculating a pH predicted value according to the first cell type of the first cell; determining whether the actual pH value of one of the first cells detected is less than a threshold value and the pH prediction value; and when the actual pH value is greater than the valve When the value is less than the above-described pH prediction value, the culture solution for replacing the first cell described above is performed. A device for generating adaptive cell culture parameters for culturing a first cell as a target cell, the device comprising: a control circuit; a processor mounted in the control circuit; and a memory mounted in the control circuit And coupled to the processor; wherein the processor is configured to execute a code stored in the memory to perform: according to the first cell type of the first cell and a target cell characteristic from a database Searching for generalized cell culture parameters to establish a basic control factor sequence listing, wherein the basic control factor sequence list includes at least a basic control factor, and a weight and a priority order corresponding to the basic control factor; The above target cell characteristics and the above basic control factor sequence table generate adaptive cell culture parameters; the first cell is cultured according to the above adaptive cell culture parameters, and the first cell is produced. Producing a culture result; and adjusting the above adaptive cell culture parameter according to the above culture result; wherein the culture result is that the first cell is cultured as a second cell, and the processor adjusts the adaptive cell culture parameter according to the culture result The method further includes: determining whether the second cell type of one of the second cells is the same as the target cell species, and determining whether the second cell characteristic of the second cell is consistent with the target cell characteristic; when the second cell type is When the target cell type is the same and the second cell characteristic is consistent with the target cell characteristic, the second cell is determined to be the target cell; when the second cell species is the same as the target cell species and the second cell characteristic is the same as the above When the target cell characteristics are not met, the above-mentioned weights of the above basic control factors in the basic control factor sequence table and the above priority order are adjusted to re-select the basic control factor having the highest weight as the above adaptive cell culture parameter; or when Second cell type and above When the standard cell types are different and the second cell characteristic does not conform to the target cell characteristic, the basic control factor sequence table is copied as a basic control factor sequence table for culturing the first cell into the second cell, and the basic form is stored. The control factor sequence listing is included in the above database. Adaptation cell culture parameters as described in item 5 of the patent application scope And the above-mentioned processor, wherein the above-mentioned generalized cell culture parameters are searched from the above-mentioned database according to the first cell type of the first cell and the target cell characteristics, to establish the basic control factor sequence table further includes: Determining, by the first cell type, whether the first cell is a known cell; and when determining that the first cell is the known cell, obtaining a basic control factor corresponding to the known cell from the database, and setting the basic control factor a weight and priority order, and storing the basic control factor, the weight and priority order corresponding to the basic control factor in the basic control factor sequence table; or when determining that the first cell is not the above known cell, storing The preset basic control factor and the weight and priority order corresponding to the preset basic control factor are in the above basic control factor sequence table. The apparatus for producing adaptive cell culture parameters according to claim 5, wherein the processor generates the adaptability according to the first cell type of the first cell, the target cell characteristic, and the basic control factor sequence table. The cell culture parameter further comprises: determining whether the first cell is a known cell according to the first cell type of the first cell; and when determining that the first cell is the known cell, obtaining the corresponding data from the database Knowing the basic control factor of the cell, selecting the basic control factor with the highest weight from each of the basic control factors as the above-mentioned adaptive fineness a cell culture parameter; or when it is determined that the first cell is not the above-mentioned known cell, the third cell similar to the first cell species of the first cell is obtained from the database, and the copy corresponds to the basic of the third cell The control factor is used as a basic control factor corresponding to the first cell, and the basic control factor having the highest weight is selected from each of the basic control factors as the adaptive cell culture parameter. The apparatus for producing adaptive cell culture parameters according to claim 5, wherein when the first cell is cultured according to the adaptive cell culture parameter, the processor further executes the above code to execute: the first cell Performing a pH concentration prediction mechanism; wherein the pH concentration prediction mechanism comprises: calculating a pH prediction value according to the first cell type of the first cell; and determining whether the actual pH value of one of the first cells detected is less than a valve And a value predicted by the pH; and when the actual value of the pH is greater than the threshold and less than the predicted value of the pH, the culture solution for replacing the first cell is performed. A storage medium, after reading a code stored in the storage medium via a device, to perform an adaptive cell culture parameter generation method for cultivating the first cell as a target cell, wherein the method comprises the following steps: One of the first cell types and a target cell characteristic of a cell is searched for by a database of generalized cell culture parameters to establish a basic control a sequence sequence table, wherein the basic control factor sequence table comprises at least a basic control factor, and a weight and a priority order corresponding to the basic control factor; generating according to the first cell type, the target cell characteristic, and the basic control factor sequence table; An adaptive cell culture parameter; cultivating the first cell according to the above adaptive cell culture parameter, and producing a culture result; and adjusting the adaptive cell culture parameter according to the culture result; wherein the culture result is that the first cell is cultured For the second cell, adjusting the adaptive cell culture parameter according to the culture result further includes: determining whether the second cell type of one of the second cells is the same as the target cell species, and determining whether the second cell characteristic of the second cell is Corresponding to the characteristics of the target cell; when the second cell species is the same as the target cell species and the second cell characteristic is consistent with the target cell characteristic, the second cell is determined to be the target cell; Cell type and upper When the target cell species are the same and the second cell characteristic does not match the target cell characteristic, the weight of the basic control factor in the basic control factor sequence table and the priority order are adjusted to reselect the basic control with the highest weight. a factor as the above adaptive cell culture parameter; or when the second cell species are different from the above target cell species and the above When the second cell characteristic does not conform to the above target cell characteristic, the above basic control factor sequence table is copied as a basic control factor sequence table for culturing the first cell into the second cell, and the basic control factor sequence table is stored to the above data. In the library. The storage medium according to claim 9, wherein the generalized cell culture parameter is searched from the database according to the first cell type of the first cell and the target cell characteristic to establish the basic control factor sequence. The list further includes: determining whether the first cell is a known cell according to the first cell type; and determining that the first cell is the known cell, obtaining a basic control factor corresponding to the known cell from the database, Setting a weight and a priority order corresponding to the basic control factor, and storing the basic control factor, a weight corresponding to the basic control factor, and a priority order in the basic control factor sequence table; or when determining that the first cell is not the above When the cell is known, the preset basic control factor and the weight and priority order corresponding to the preset basic control factor are stored in the above basic control factor sequence table. The storage medium according to claim 9, wherein the obtaining the adaptive cell culture parameter according to the first cell type of the first cell, the target cell characteristic, and the basic control factor sequence table further comprises: according to the above The first cell type of the above cell determines the first fine Whether the cell is a known cell; when it is judged that the first cell is the above known cell, the basic control factor corresponding to the known cell is obtained from the above database, and the basic control having the highest weight is selected from each of the basic control factors a factor as the above adaptive cell culture parameter; or when it is determined that the first cell is not the above-mentioned known cell, the third cell similar to the first cell type of the first cell is obtained from the database, and the copy corresponds to the above The basic control factor of the third cell is used as a basic control factor corresponding to the first cell, and the basic control factor having the highest weight is selected from each of the basic control factors as the adaptive cell culture parameter. The storage medium according to claim 9, wherein the cultivating the first cell according to the adaptive cell culture parameter further comprises: performing a pH concentration prediction mechanism on the first cell; wherein the pH concentration prediction mechanism comprises: Calculating a pH predicted value according to the first cell type of the first cell; determining whether the actual pH value of one of the first cells detected is less than a threshold value and the pH prediction value; and when the actual pH value is greater than the valve When the value is less than the above-described pH prediction value, the culture solution for replacing the first cell described above is performed.