TWI635279B - The methods of detecting cancer stem cells in vitro - Google Patents

Abstract

The present invention is a method for detecting cancer stem cells in vitro by adding two different liquid samples to a vessel containing a growth substrate via an injection device to generate a test sample; and then transferring the test sample to the detection system to generate a detection result. One of the liquid samples contains cancer cells or a cell growth matrix, and the other liquid sample contains chemicals. In the method, the injection device comprises a dropper and a controller for regulating the injection volume, and the detection system comprises a light source, a detection device and a control device. The detecting device receives the fluorescent signal from the test sample and converts the fluorescent signal into a data to the control device to generate a detection result.

Description

Method for detecting cancer stem cells in vitro

The present invention relates to a method for detecting cancer stem cells in vitro, and is a technique for easily determining the presence of cancer stem cells.

Cancer stem cells, English: Cancer Stem Cell, also known as cancer stem cells, cancer stem cells, refers to the characteristics of stem cell properties. Cancer stem cells have the ability to self-renewal and multi-cell differentiation. Generally, cancer cells do not have the ability to self-renew and multi-cell differentiation. Cancer stem cells are considered to cause cancer metastasis, recurrence, or tumor resistance to chemotherapy and radiotherapy. And cancer cells have different names in different organ tissues such as blood cancer, skin cancer, brain cancer, oral cancer, liver cancer, breast cancer, thyroid cancer, lung cancer, pancreatic cancer, esophageal cancer, stomach cancer, colon cancer, prostate cancer, ovary Cancer, cervical cancer, etc. Cancer stem cells exist in various cancer cells, and the autofluorescence characteristics of cancer stem cells are also distinct from those of cancer cells (non-staining agent fluorescence). Moreover, cancer stem cells have stem cell markers such as CD34, CD44, and CD133 cell surface specific molecules, which are not found in cancer cells.

At present, the method for detecting cancer cells is to detect by using an antibody. The method is to synthesize a signal substance to be combined with an antibody by artificial synthesis, and the antibody is adhered to the surface substance of the cancer cell. After a series of treatments, the detection is from The signal generated by antibodies adhering to the surface of cancer cells is analyzed. There have been no non-antibody adhesion to the surface of cancer cells to detect specific cancer Cell method.

The present invention is directed to a method for detecting cancer stem cells, and the invention of the present invention proposes a method for detecting cancer stem cells in vitro.

In order to achieve the above object, the method for detecting cancer stem cells in vitro comprises the following steps: Step one, in a vessel containing a cell growth substrate via an injection device, the source of the injection is taken from a mammal or a normal human cell, a cancer stem cell, The first liquid sample of the cancer cell and the source obtain the second liquid sample of the appropriate amount and the appropriate concentration of the chemical drug to become the test sample; and in step 2, the test sample is placed in the detection system to produce the detection result.

In the first step, the first liquid sample contains an appropriate amount of an appropriate concentration of anthracyclines, anthraquinones or quinolines, which may be synthetic or natural extraction. The injection device contains a single or multiple dropper (Figure 4) and a controller to regulate the amount of liquid sample dripped.

In step 2, the detection system includes at least one laser light source to induce cancer stem cells and cancer cells to emit different autofluorescence instead of the fluorescence of the chemical itself, and the detecting device can detect and distinguish the different fluorescent lights. The control device is responsible for converting the data obtained by the detecting device into the detection result report.

1‧‧‧Control device interface for injection devices

2‧‧‧Injection device

3‧‧‧ Dropper

4‧‧‧ utensils or utensils

5‧‧‧Light source

6‧‧‧Detection device

7‧‧‧Detection system

8‧‧‧Control device

9‧‧‧Control device operation interface

1 is a schematic view showing a schematic view of an embodiment of the present invention; FIG. 2 is a front view of a schematic view of an embodiment of the present invention; [Fig. 3] is a left side view of a schematic view of an injection device according to an embodiment of the present invention; [Fig. 4] a schematic diagram of a single or a plurality of droppers of a series of injection devices; [Fig. 5] is a view showing an investigation according to an embodiment of the present invention. Schematic diagram of the system.

Fig. 6 is a diagram showing the autofluorescence of cancer stem cells according to an embodiment of the present invention.

The characteristics of the creation of this case can be understood by referring to the diagram of the case and the description of the embodiment.

A method of detecting in vitro cancer stem cells as shown in the first embodiment is shown in FIG.

In the first step of the embodiment, the vessel 4 containing the cell growth substrate is added to the source from the mammalian mouse via the injection device 2 having the two dropper 3, and the first liquid sample contains normal cells, cancer stem cells, The cancer cells, the cell growth substrate, and the second liquid sample contain appropriate concentrations of anthracycline chemical components, and a controller is used to regulate the amount of the drop to make it a test sample. The controller has a user interface 1.

Step 2: After the two liquid samples are added, the vessel is placed on the laser light source 5 in the detection system, and the data is obtained through the detecting device and converted to generate a detection result report. Figure 6 shows the autofluorescence of cancer stem cells.

In summary, the creation of the case provides a better and feasible method for detecting cancer stem cells in vitro, and submits an application for a patent for invention according to law; the technical content and technical features of the creation have been disclosed above, but those familiar with the technology may still Based on the disclosure of this creation, various substitutions and modifications are made without departing from the spirit of the creation of the case. Therefore, the scope of protection of this creation should not be limited to those disclosed in the examples, but should include various substitutions and modifications without departing from the scope of the invention, and the scope of the following patent application Covered.

Claims (5)

A method for detecting cancer stem cells in vitro, comprising: using a liquid sample taken from a mammalian cell or a human cell, wherein the chemical substance contained therein is filled with the characteristics of the cancer stem cell, and the characteristic is permeable to Detection system detection. For example, in the chemical substance described in the scope of the patent application [Item 1], the chemical substance may contain an appropriate amount of an anthracene ring such as [Chemical Formula 1] or an anthracene such as [Chemical Formula 2] or [Chemical Formula 3] Quinoline chemical composition. For example, in the detection system described in the scope of claim [1], the detection system can emit appropriate excitation wavelength and detection devices and control devices for at least one laser light source. For example, in the detecting device described in the scope of claim [3], the detecting device may be composed of at least one detector, which is capable of receiving the appropriate divergent wavelength of the sample under test to generate a fluorescent signal and converting the fluorescent light. Signal into information. For example, in the control device described in the scope of the patent application [Item 3], the function of the control device is to control the detection system and generate the detection result according to the data described in the patent application scope [item 4].