TWI616203B - Use of bilberry extraction in preparing pharmaceutical composition for protecting and repairing mitochondria,improving an ability of the mitochondria to perform oxidative phosphorylation and synthesize adenosine triphosphate,increasing spare respiratory - Google Patents

Abstract

A mulberry extract is used for preparing protection and repairing mitochondria, improving the ability of mitochondria to undergo oxidative phosphorylation and ATP synthesis, improving the pre-existing oxygen consumption capacity of mitochondria, and improving mitochondria Use of a pharmaceutical composition for basal oxygen consumption of an oxidative phosphorylation reaction for synergistic efficiency of synthesizing triphosphates. Mangosteen extract contains Anthocyanidins. When the pharmaceutical composition is provided to the cells, the mulberry extract is provided to the cells as an aqueous solution of the mulberry extract. Bilberry extract concentration of the aqueous solution is from 25 to 250 micrograms per ml (μ g / ml).

Description

Mulberry extract is used to prepare and protect mitochondria, improve the ability of mitochondria to undergo oxidative phosphorylation and triphosphate synthesis, increase the pre-existing oxygen consumption capacity of mitochondria, and improve the efficiency of mitochondria. Use of pharmaceutical composition

The invention relates to the use of a mulberry seed extract for preparing a pharmaceutical composition, in particular to a mulberry seed extract for preparing protection and repair of mitochondria, improving mitochondria for oxidative phosphorylation reaction and triphosphate glucoside. Use of a pharmaceutical composition capable of synthesizing, improving pre-stored oxygen consumption capacity of mitochondria, and improving mediation efficiency of mitochondria.

Mitochondria is the main site for oxidative phosphorylation and synthesis of adenosine triphosphate (ATP) in cells. Because adenosine triphosphate is the source of energy for cellular activities, mitochondria are also known as "cell energy factories". In addition to providing energy to cells, mitochondria are also involved in processes such as cell differentiation, cell information transmission, and apoptosis, and have the ability to regulate the cell growth cycle.

However, some of the by-products produced by mitochondria during oxidative phosphorylation are harmful to the inner membrane of mitochondria. Over a long period of time, the severely damaged mitochondrial inner membrane will trigger mitochondrial disintegration, and then trigger apoptosis. Therefore, how to repair and protect mitochondria and thereby slow down the rate of apoptosis triggered by mitochondrial disintegration has become an important issue.

The invention provides a mulberry seed extract for preparing protection and repairing mitochondria, improving the ability of mitochondria to undergo oxidative phosphorylation and synthesizing triphosphates, improving the pre-existing oxygen consumption capacity of mitochondria, and improving mitochondria. The use of the pharmaceutical composition of the mediating efficiency of the body, thereby delaying the rate of apoptosis triggered by mitochondrial disintegration.

According to an embodiment of the present invention, a mulberry seed extract is used to prepare and protect mitochondria, improve the ability of mitochondria to undergo oxidative phosphorylation and synthesize ATP, and increase the pre-existing oxygen consumption of mitochondria. The use of a pharmaceutical composition for improving the efficiency of mediation of the oxidative phosphorylation of mitochondria for the oxidative phosphorylation of mitochondria for synthesizing the efficiency of synthesizing triphosphates; wherein the extract of the mulberry seed contains anthocyanidins; Wherein, when the medicinal composition is provided to a cell, the mulberry extract is provided to the cell in the form of an aqueous solution of mulberry extract, and the concentration of the aqueous solution of the mulberry extract is 25 to 250 micrograms per milliliter (μg / ml).

According to another embodiment of the present invention, a mulberry seed extract is used for synthesis to protect and repair mitochondria, improve the pre-stored oxygen consumption capacity of mitochondria, and increase the basic oxygen consumption of oxidative phosphorylation of mitochondria. The use of a medicinal composition for the mediation efficiency of linosine triphosphate, wherein the extract of the mulberry seed contains anthocyanins, and the effective amount of the extract of the mulberry seed is 0.27 g to 2.7 g.

The extract of mulberry seeds disclosed in the present invention is used to prepare and protect mitochondria, improve the ability of mitochondria to undergo oxidative phosphorylation and synthesize ATP, and increase the pre-existing oxygen consumption of mitochondria. Capability and use of a pharmaceutical composition for improving the efficiency of mitochondrial oxidative phosphorylation in the basic oxygen consumption of mitochondria for synthesizing triphosphate glycosides, providing mulberry seeds extract to cells to protect and repair mitochondria The inner membrane is used to delay the disintegration of mitochondria. In this way, the rate at which mitochondrial disintegration triggers apoptosis can be slowed.

The above description of the content of this disclosure and the description of the following embodiments are used to demonstrate and explain the spirit and principle of the present invention, and to provide more scope for the patent application of the present invention. Further explanation.

FIG. 1 is a schematic diagram of oxygen consumption of synthetic triphosphates in Examples 1 to 3 and Comparative Examples 1 to 3. FIG.

FIG. 2 is a schematic diagram of the basic oxygen consumption of the mitochondria of Examples 1 to 3 and Comparative Examples 1 to 3. FIG.

FIG. 3 is a schematic diagram of oxygen consumption for overcoming hydrogen ion leakage in Examples 1 to 3 and Comparative Examples 1 to 3. FIG.

FIG. 4 is a schematic diagram of the maximum oxygen consumption capacity of the mitochondria of Examples 1 to 3 and Comparative Examples 1 to 3. FIG.

FIG. 5 is a schematic diagram of the pre-stored oxygen consumption capacity of the mitochondria of Examples 1 to 3 and Comparative Examples 1 to 3. FIG.

FIG. 6 is a schematic diagram of the mitochondrial triphosphate glycoside mediation efficiency of the mitochondria in Examples 1 to 3 and Comparative Examples 1 to 3. FIG.

The detailed features and advantages of the present invention are described in detail in the following embodiments. The content is sufficient for any person skilled in the art to understand and implement the technical content of the present invention, and according to the content disclosed in this specification, the scope of patent applications and the drawings. Anyone skilled in the relevant art can easily understand the related objects and advantages of the present invention. The following examples further illustrate the viewpoints of the present invention in detail, but do not limit the scope of the present invention in any way.

Bilberry, also known as bilberry, is a collective name for a variety of dwarf deciduous shrubs of the genus Vaccinium of the azalea family with blue-black or blue-purple berries, mainly distributed in Europe, North America (United States and Canada), and Asia North. Berries of mountain mulberry are produced in summer and autumn. Mulberry seeds are rich in various bioflavonoids, including anthocyanidin, delphinidin, peonydin, petunidin, pelargonidin, and cyanidin And its glycosides.

The bioflavonoids contained in mulberry seeds have the effect of promoting the synthesis of rhodopsin by 11-trans-Rentinal and Opsin on the retina. The rhodopsin on the retina is the subject of the visual transmission system. When rhodopsin is irradiated with light, 11-cis-Retinal inside rhodopsin is excited by light and transformed into 11-trans-Rentinal, making rhodopsin Activated, and then through a series of chain reactions to produce visual signals transmitted in the optic nerve. (11-trans-Rentinal) in activated rhodopsin is then transformed into 11-trans-Rentinal through the action of enzymes, and recombines with opsin to form rhodopsin under the action of bioflavonoids quality. Due to the variety of bioflavonoids contained in mulberry seeds, mulberry seeds are often added to vision health products.

The scientific name of the mountain mulberry used in the present invention is Vaccinium uliginosum L. The way to obtain the mulberry extract is to extract the mulberry fruit with carbon dioxide as a supercritical fluid, or use methanol, ethanol, acetone, ethyl acetate, 0.1 to 5% by weight sodium chloride aqueous solution, and potassium chloride aqueous solution. , Sodium chloride aqueous solution, magnesium chloride aqueous solution or 0.1 to 5% by weight sodium chloride ethanol solution, potassium chloride ethanol solution, calcium chloride ethanol solution, magnesium chloride ethanol solution, ethanol solution as solvents Extraction. Next, the primary extract is filtered and purified to obtain the mulberry seed extract used in the present invention.

When an aqueous solution of Mulberry seed extract is provided to cells at a concentration of 25 to 250 micrograms (μg / ml) per milliliter, the Mulberry seed extract entering the cells can protect and repair the inner membrane of mitochondria. In this way, the efficiency of oxidative phosphorylation reaction to synthesize glucosides of triphosphate is improved in the inner membrane of mitochondria. In detail, the amount of triphosphates synthesized by oxidative phosphorylation of mitochondria repaired with extract of mulberry seeds increased, the basic oxygen consumption of mitochondria increased, and the amount of hydrogen ion leakage in the inner membrane of mitochondria decreased, and The maximum oxygen consumption capacity of the mitochondria is increased, the pre-stored oxygen consumption capacity of the mitochondria is increased, and the mitochondrial triphosphate glycoside mediation efficiency is improved.

A method of providing a mangosteen extract to a cell is, for example, ingesting the mangosteen extract by mouth. When mulberry seeds are provided to cells in an edible manner, The effective dose of the sub-extract is 0.27 grams (g) to 2.7 grams. The effective dose here is calculated based on the conversion formula between the effective dose of the cell experiment and the kilogram of the human body. The conversion formula is as follows: effective dose for human body = effective dose for cell experiments × mouse weight × conversion factor × human kilograms. The conversion factor is obtained from a look-up table of dose conversion coefficients per kilogram of body weight of animals and humans. When the mouse weighs 20 grams and the human kilogram weighs 60 kilograms, the conversion factor is 9.01.

In order to facilitate the ingestion of the wild mulberry extract from the mouth, the wild mulberry extract can be made into, for example, a liquid, solid, granular, powdery, pasty, or gelled processed wild mulberry extract. The processed extract of mulberry seeds can be used as an excipient or flavoring agent as an additive to enhance the flavor and ease of consumption.

Excipients are, for example, starches such as wheat starch, rice starch, corn starch, potato starch, dextrin, and cyclodextrin; crystalline celluloses; lactose, glucose, granulated sugar, reduced maltose, caramel, fructooligosaccharides, and emulsified oligosaccharides And other sugars; sorbitol, erythritol, xylitol, lactitol, mannitol and other sugar alcohols.

Flavoring agents are, for example, various fruit extracts such as longan extract, lychee extract, grapefruit extract; various fruit juices such as apple juice, orange juice, and lemon juice; various spices such as peach flavor, plum flavor, and yogurt flavor; acesulfame Various sweeteners such as potassium acid, sucralose, erythritol, oligosaccharides, mannose, xylitol, isomerized sugars; various acidifiers such as citric acid, malic acid, tartaric acid, gluconic acid; green tea, oolong tea, Various tea ingredients such as Banaba Tea, Eucommia Tea, Tieguanyin Tea, 薏苡 Tea, Buckeye Tea, 胆 白茶, Kunbu Tea, etc.

Furthermore, the extract of mulberry seeds can also be coated in capsules to facilitate the ingestion of mulberry seeds. The wild mulberry extract can be coated in hard capsules in the form of a dry powder, or the wild mulberry extract can be coated in soft capsules in the form of a solution, suspension, paste, powder or granule.

The oils and fats used in the soft capsules to dissolve or disperse the extract of mulberry seeds are, for example, argan oil, almond oil, linseed oil, cumin oil, white perilla oil, olive oil, olive squalene, Sweet orange oil, orange roughy oil, sesame oil, garlic oil, cocoa butter, pumpkin seed oil, chamomile oil, carrot oil, courgette oil, tallow fatty acids, Hawaiian stone oil, bilberry oil, brown rice germ oil , Rice oil, wheat germ oil, safflower oil, shea oil, liquid shea oil, perilla oil, soybean oil, evening primrose oil, camellia oil, corn oil, rapeseed oil, saw palmetto extract (saw palmetto extract oil), emu oil, peach kernel oil, parsley oil, castor oil, sunflower oil, grape seed oil, borage oil, Australian walnut oil, meadowfoam oil, cottonseed oil, peanut oil, turtle oil, Mink oil, egg butter, fish oil, palm oil, palm kernel oil, wood wax, coconut oil, long chain / medium chain / short chain fatty acid triglycerides, diglycerides, tallow, lard, squalene, Squalane, pinane, and hydrides of these oils and fats. Among them, borage oil and evening primrose oil contain a large amount of Gamma-Linolenic Acid (GLA). Gamma-linolenic acid is an essential fatty acid of the human body, which has moisturizing, promotes cell regeneration, and promotes brown fat activity. Degree to promote fat burning function.

In addition, colorants, preservatives, tackifiers, binding agents, disintegrating agents, dispersants, stabilizers, gelling agents, antioxidants, surfactants, preservatives, pH adjusters, etc. are added in accordance with government regulations. It can also be added to the processed products of mulberry seed extract according to the dosage standard stipulated by the government unit and the requirements of processing production.

In the following, the methods for protecting and repairing mitochondria disclosed by the present invention will be described by using examples 1 to 3 and comparative examples 1 to 3 of the present invention, and experimental tests will be performed to explain the protection and repair of mitochondria disclosed by the present invention. The efficacy of the method.

The extract of mountain mulberry used in the experiment of the present invention is obtained by extracting and drying the fruit of mountain mulberry ( Vaccinium uliginosum L. ) with an aqueous ethanol solution. The dried mulberry extract is in the form of Violet Red Powder, with a density of 0.45 to 0.70 grams per milliliter (0.45 to 0.70 g / ml). The powder can pass through a mesh of 80 mesh. The extract of mountain mulberry contains anthocyanidins with an concentration of more than 25% by weight.

The cells used in the experiment were the sixth generation (P6) adipose mesenchymal stem cells (ADSC). The experimental sample was prepared by implanting 8000 adipose mesenchymal stem cells into each well of the well plate and culturing for 24 hours.

In the experiment, the mitochondrial damage was simulated by exposing the cells to a 200 mM aqueous solution of hydrogen peroxide (H ₂ O ₂ ) for 30 minutes, followed by washing with phosphate buffered saline (PBS). cell. Because the oxidizing power of hydrogen peroxide can cause damage to the inner membrane of the mitochondrial membrane, the oxidizing power of hydrogen peroxide is used to damage the inner membrane of the mitochondrial membrane in the experiment, and the oxidative power of free radicals in the body is caused to the inner membrane of the mitochondrial membrane damage.

In the course of the experiment, an aqueous solution of a mulberry seed extract of a predetermined concentration was first added to the wells and soaked for 24 hours. Next, a 200 mM H ₂ O ₂ aqueous solution was added to the wells, and the cells were immersed in a 200 mM H ₂ O ₂ aqueous solution for 30 minutes. Next, the cells were washed with phosphate buffered saline (PBS). Finally, the hippocampal bioenergy meter was used to measure the oxygen consumption of cells in the wells.

In Example 1, the concentration of the aqueous solution of Mulberry seed extract was 25 micrograms (μg / ml) per milliliter. In Example 2, the concentration of the aqueous solution of Mulberry seed extract was 50 micrograms (μg / ml) per milliliter. In Example 3, the concentration of the aqueous solution of Mulberry seed extract was 250 micrograms (μg / ml) per milliliter. In Comparative Example 1, an aqueous solution of mulberry seed extract was not added to the pores of the perforated disk. In Comparative Example 2, the concentration of the aqueous solution of Mulberry seed extract was 12.5 micrograms (μg / ml) per milliliter. In Comparative Example 3, the concentration of the aqueous solution of the mulberry extract was 500 micrograms (μg / ml) per milliliter.

The measurement principle and process of the hippocampal bioenergy meter are as follows. First, the basal oxygen consumption of the cells in the well is detected. Next, a triphosphate synthase inhibitor is added to inhibit mitochondria from producing triphosphate, and the reduced oxygen consumption at this time is the oxidative phosphorylation reaction of the mitochondria to synthesize the oxygen consumption of triphosphate. This is the basic oxygen consumption (Basal Respiration) of the mitochondria. An inhibitor of triphosphate glycoside synthase is, for example, Oligomycin. Next, add an appropriate concentration of anti-coupling agent to prevent the electron transport chain in the inner membrane of the mitochondria from breaking. In this case, the mitochondria were allowed to idle under extreme conditions to assess the maximum oxygen consumption capacity of the mitochondria (Maximal Respiration). The anti-coupling agent is, for example, Carbonyl cyanide-4- (trifluoromethoxy) phenylhydrazone (FCCP). Finally, the addition of an electron transfer chain inhibitor has completely turned off mitochondrial oxygen consumption, thereby confirming the measured background value, that is, non-mitochondrial oxygen consumption (Non-mitochondrial Respiration). The electron transport chain inhibitor is, for example, a combination of Rotenone and Antimycin A.

The basal oxygen consumption of the mitochondria is equal to the basal oxygen consumption of the cells minus the non-mitochondrial oxygen consumption. The basic oxygen consumption of the mitochondria minus the amount of oxygen consumed by the synthesis of linear glucosides of triphosphate is equal to the oxygen consumption to overcome the hydrogen ion leakage (Proton Leakage). The maximum oxygen consumption capacity of mitochondria minus the basic oxygen consumption of mitochondria is equal to the Spare Respiratory Capacity of mitochondria. The mitochondrial triphosphate glycoside mediation efficiency (Coupling Efficiency) is equal to the oxygen consumption of the synthetic triphosphate glycoside divided by the mitochondrial base oxygen consumption.

The concentration and experimental measurement results of the aqueous solution of the mulberry seed extract in Examples 1 to 3 and Comparative Examples 1 to 3 are shown in Table 1 and FIGS. 1 to 6. The experimental measurement results of the oxygen pmoles per microgram protein per minute (pmol / min / μg protein) presented in Table 1 are the experimental measurement results after the cell mass has been standardized. FIG. 1 is a schematic diagram of oxygen consumption of synthetic triphosphates in Examples 1 to 3 and Comparative Examples 1 to 3. FIG. FIG. 2 is a schematic diagram of the basic oxygen consumption of the mitochondria of Examples 1 to 3 and Comparative Examples 1 to 3. FIG. FIG. 3 is a schematic diagram of oxygen consumption for overcoming hydrogen ion leakage in Examples 1 to 3 and Comparative Examples 1 to 3. FIG. FIG. 4 is a schematic diagram of the maximum oxygen consumption capacity of the mitochondria of Examples 1 to 3 and Comparative Examples 1 to 3. FIG. FIG. 5 is a schematic diagram of the pre-stored oxygen consumption capacity of the mitochondria of Examples 1 to 3 and Comparative Examples 1 to 3. FIG. FIG. 6 is a schematic diagram of the mitochondrial triphosphate glycoside mediation efficiency of the mitochondria in Examples 1 to 3 and Comparative Examples 1 to 3. FIG.

Table I

As shown in FIG. 1, the oxygen consumption of the synthetic triphosphates in Examples 1 to 3 is higher than that in Comparative Examples 1 to 3. As shown in FIG. 2, the basic oxygen consumption of the mitochondria of Examples 1 to 3 is higher than that of Comparative Examples 1 to 3. As shown in FIG. 3, the oxygen consumption for overcoming hydrogen ion leakage in the second and third embodiments is lower than that of the first to the third comparative examples. Figure 4 As shown, the maximum oxygen consumption capacity of the mitochondria of Examples 1 to 3 is higher than that of Comparative Examples 1 to 3. As shown in FIG. 5, the pre-stored oxygen consumption capacity of the mitochondria of Examples 1 to 3 is higher than that of Comparative Examples 1 to 3. As shown in FIG. 6, the mitochondrial triphosphate glycoside mediation efficiency of the mitochondria of Examples 1 to 3 is higher than that of Comparative Examples 1 to 3. Therefore, it can be known from FIG. 1 to FIG. 6 that the increased basic oxygen consumption of the mitochondria in Examples 1 to 3 is mainly used for synthesizing triphosphates, which increases the synthesis amount of triphosphates, that is, mitochondria The efficiency of thioglycoside triphosphate is improved. At the same time, the leakage amount of hydrogen ions in the inner membrane of the mitochondria decreased in the second and third embodiments, that is, the amount of oxygen consumed by the mitochondria to retransmit hydrogen ions to the membrane space decreased, which represented the damage of the inner membrane of the mitochondria. The condition was improved by the repair of the extract of mulberry seeds.

According to the results of the above experimental test, the mitochondria treated with an aqueous solution of mulberry seeds at a concentration of 25 micrograms to 250 micrograms per milliliter (μg / ml) are protected by the berry extract to reduce the damage of the inner membrane of the mitochondria to oxidants. . Therefore, an aqueous solution of mulberry seed extract at a concentration of 25 micrograms to 250 micrograms (μg / ml) per milliliter has the effect of protecting mitochondria.

At the same time, the mitochondrial inner membrane of the mitochondria treated with an aqueous solution of the mulberry extract at a concentration of 50 to 250 micrograms per milliliter (μg / ml) was also repaired by the mulberry extract, causing the self-membrane gap to break through The amount of hydrogen ions leaking from the inner membrane to the matrix decreases, which in turn reduces the amount of oxygen consumed by the mitochondria to retransmit hydrogen ions to the membrane space. Therefore, in addition to the effect of protecting mitochondria, the aqueous solution of extract of mulberry seeds at a concentration of 50 to 250 micrograms (μg / ml) per milliliter has the effect of repairing mitochondria.

However, mitochondria that have not been treated with an aqueous solution of Japanese mulberry extract are not protected by an extract of Japanese mulberry seeds, and mitochondria treated with an aqueous solution of Japanese mulberry extract at a concentration of 12.5 micrograms (μg / ml) per milliliter are protected. It is not enough that the inner membrane of mitochondria is damaged by oxidants. Furthermore, the mitochondria treated with an aqueous solution of mulberry extract at a concentration of 500 micrograms (μg / ml) per milliliter, because the excessively concentrated aqueous solution of mulberry extract caused more damage to the inner membrane of the mitochondria than protection and repair. Mitochondrial capacity, the basic oxygen consumption, maximum oxygen consumption capacity, pre-stored oxygen consumption capacity, and the efficiency of mitochondrial triphosphate mediation in the mitochondria of Comparative Example 3 were implemented. The basic oxygen consumption, maximum oxygen consumption capacity, pre-existing oxygen consumption capacity, and the efficiency of mitochondrial triphosphate glycosides of the mitochondria of Examples 1 to 3 are reduced. The oxygen consumption of the mitochondria of Comparative Example 3 to overcome hydrogen ion leakage is reduced. Compared with the mitochondria of Examples 2 and 3, the oxygen consumption to overcome the leakage of hydrogen ions increases.

The extract of mulberry seeds disclosed in the present invention is used for preparing protection and repairing mitochondria, improving the ability of mitochondria to undergo oxidative phosphorylation and synthesizing triphosphates, improving the pre-existing oxygen consumption capacity of mitochondria, and improving The use of the pharmaceutical composition of the mitochondrial mediation efficiency, to provide the extract of the mulberry seed to the cells to protect and repair the inner membrane of the mitochondria to delay the time for the mitochondria to disintegrate. In this way, the rate at which mitochondrial disintegration triggers apoptosis can be slowed.

Although the present invention is disclosed in the foregoing embodiments, it is not intended to limit the present invention. Changes and modifications made without departing from the spirit and scope of the present invention belong to the patent protection scope of the present invention. For the protection scope defined by the present invention, please refer to the attached patent application scope.

Claims (10)

A mulberry extract is used for preparing protection and repairing mitochondria, improving the ability of mitochondria to undergo oxidative phosphorylation and ATP synthesis, improving the pre-existing oxygen consumption capacity of mitochondria, and improving mitochondria Use of a medicinal composition for synthesizing the efficiency of synthesizing triphosphates in a basic oxygen consumption amount for performing an oxidative phosphorylation reaction, wherein the mulberry extract contains anthocyanidins; wherein, when the medicinal composition When an extract is provided to a cell, the extract of the mulberry seed is provided to the cell in the form of an aqueous solution of the berry extract, and the concentration of the aqueous solution of the berry extract is 25 to 250 micrograms per milliliter (μg / ml). A berry extract used for the preparation and protection of mitochondria, improving the pre-existing oxygen consumption capacity of mitochondria and improving the basic oxygen consumption of oxidative phosphorylation reaction of mitochondria for synthesizing triphosphate glycosides The use of an efficient pharmaceutical composition, wherein the mulberry extract contains anthocyanins, and the effective amount of the mulberry extract is 0.27 g to 2.7 g. The Mulberry Seed Extract as described in claim 1 is used to prepare and protect mitochondria, improve the ability of mitochondria to undergo oxidative phosphorylation and synthesize ATP, and increase the pre-existing oxygen consumption of mitochondria. The use of the pharmaceutical composition for improving the efficiency of mitochondrial oxidative phosphorylation in the basic oxygen consumption of mitochondria for synthesizing the efficiency of synthesizing triphosphates, wherein the mulberry extract contains a concentration of 25% by weight or more. anthocyanin. The Mulberry Seed Extract as described in claim 1 is used to prepare and protect mitochondria, improve the ability of mitochondria to undergo oxidative phosphorylation and synthesize ATP, and increase the pre-existing oxygen consumption of mitochondria. Used in the synthesis of mitochondria to increase the basic oxygen consumption of oxidative phosphorylation The use of a pharmaceutical composition for the mediation efficiency of phosphinosides, wherein the concentration of the aqueous solution of the mulberry seed extract is 50-250 micrograms (μg / ml) per milliliter. The Mulberry Seed Extract as described in claim 1 is used to prepare and protect mitochondria, improve the ability of mitochondria to undergo oxidative phosphorylation and synthesize ATP, and increase the pre-existing oxygen consumption of mitochondria. The use of a pharmaceutical composition for improving the efficiency of mitochondrial oxidative phosphorylation in basal oxygen consumption of mitochondria for synthesizing the efficiency of synthesizing triphosphates, wherein the step of providing the mulberry seed extract to the cells comprises eating the Mountain Mulberry Extract. The mulberry extract as described in claim 5 is used to prepare and protect mitochondria, improve the ability of mitochondria to undergo oxidative phosphorylation and synthesize ATP, and increase the pre-existing oxygen consumption of mitochondria. The use of a pharmaceutical composition for improving the efficiency of mitochondrial oxidative phosphorylation in the basic oxygen consumption of mitochondria for synthesizing the efficiency of synthesizing triphosphates, wherein the effective amount of the edible mulberry extract is 0.27 g ( g) to 2.7 grams. The Mulberry Seed Extract as described in claim 6 is used to prepare and protect mitochondria, improve the ability of mitochondria to undergo oxidative phosphorylation and synthesize ATP, and increase the pre-existing oxygen consumption of mitochondria. Capability and use of a pharmaceutical composition for improving the efficiency of mitochondrial oxidative phosphorylation in the basic oxygen consumption of synthesizing triphosphates, wherein the mulberry extract is coated in soft or hard capsules in. The mulberry extract as described in claim 1 is used to prepare and protect mitochondria, improve the ability of mitochondria to undergo oxidative phosphorylation and synthesize ATP, and increase the pre-existing oxygen consumption of mitochondria The use of a pharmaceutical composition for improving the efficiency of mitochondrial oxidative phosphorylation in the basic oxygen consumption of mitochondria for synthesizing the efficiency of synthesizing triphosphates, wherein the mulberry extract enhances the mitochondria to perform the oxidation This basic oxygen consumption of the phosphorylation reaction. The Mulberry Seed Extract as described in claim 1 is used to prepare and protect mitochondria, improve the ability of mitochondria to undergo oxidative phosphorylation and synthesize ATP, and increase the pre-existing oxygen consumption of mitochondria. The use of a pharmaceutical composition for improving the efficiency of mitochondrial oxidative phosphorylation in the basic oxygen consumption of mitochondria for synthesizing the synergistic efficiency of triphosphates, wherein the extract of the mulberry seed enhances the adenosine monophosphates of the mitochondria Maximum limit production. The Mulberry Seed Extract as described in claim 1 is used to prepare and protect mitochondria, improve the ability of mitochondria to undergo oxidative phosphorylation and synthesize ATP, and increase the pre-existing oxygen consumption of mitochondria. Capability and use of a pharmaceutical composition for improving the efficiency of mediation of oxidative phosphorylation of mitochondria for oxidative phosphorylation of mitochondrial triphosphates, wherein the mulberry extract is in the form of an aqueous solution of the mulberry extract Provided to the cells, when the concentration of the aqueous solution of the wild mulberry extract was 50 to 250 micrograms (μg / ml) per milliliter, the wild mulberry extract reduced a hydrogen ion leakage of the mitochondria (Proton Leakage).