TWI592492B - Microbial Detecting System - Google Patents
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- 230000000813 microbial effect Effects 0.000 title claims description 22
- 238000001514 detection method Methods 0.000 claims description 43
- 239000012530 fluid Substances 0.000 claims description 26
- 230000007246 mechanism Effects 0.000 claims description 12
- 244000005700 microbiome Species 0.000 claims description 11
- 239000011521 glass Substances 0.000 claims 2
- 230000001580 bacterial effect Effects 0.000 description 3
- FGRBYDKOBBBPOI-UHFFFAOYSA-N 10,10-dioxo-2-[4-(N-phenylanilino)phenyl]thioxanthen-9-one Chemical compound O=C1c2ccccc2S(=O)(=O)c2ccc(cc12)-c1ccc(cc1)N(c1ccccc1)c1ccccc1 FGRBYDKOBBBPOI-UHFFFAOYSA-N 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Description
本發明係與檢測系統有關,特別是有關於一種微生物檢測系統。The present invention relates to detection systems, and more particularly to a microbial detection system.
按,一般欲自一待檢測物(如食物、水源或細菌培養液)檢測出其所含之微生物量時,會先自待檢測物取一小部分為採集樣本並放置於一顯微裝置進行檢測,如此即可藉由顯微裝置觀察出待檢測物之微生物量。Press, generally want to detect the amount of microorganisms contained in a test substance (such as food, water source or bacterial culture solution), first take a small part of the sample to be collected and place it in a microscopic device. Detection, so that the amount of microorganisms of the analyte to be detected can be observed by the microscopic device.
然而,如待檢測物係裝設於一與外界隔絕之封閉裝置(如細菌培養槽或是食物殺菌槽)內時,當欲進行檢測時,即必須將封閉裝置打開,才能取出一小部分之待檢測物為採集樣本進行檢測,而如此取出過程則容易導致當封閉裝置打開時,外界之微生物及髒污會不慎進入封閉裝置內而污染到位於封閉裝置內之待檢測物。另外,由於採集樣本係於外界進行檢測,如此難保採集樣本不會被外界汙染而影響到檢測結果之準確性,存在亟待改善之缺弊。However, if the object to be detected is installed in a closed device (such as a bacterial culture tank or a food sterilization tank) that is isolated from the outside world, when the test is to be performed, the closure device must be opened to take out a small portion. The object to be detected is tested for collecting samples, and the removal process is likely to cause the microorganisms and dirt outside to inadvertently enter the device to be contaminated to the object to be detected in the device when the device is opened. In addition, since the collected samples are detected by the outside world, it is difficult to ensure that the collected samples are not polluted by the outside world and affect the accuracy of the test results, and there is a drawback that needs to be improved.
因此,有必要提供一種新穎且具有進步性之微生物檢測系統,以解決上述之問題。Therefore, it is necessary to provide a novel and progressive microbial detection system to solve the above problems.
本發明之主要目的在於提供一種微生物檢測系統,可更準確地檢測待檢測物並且可有效防止封閉式之待檢測裝置及待檢測物被外界所汙染。The main object of the present invention is to provide a microorganism detecting system which can detect the object to be detected more accurately and can effectively prevent the closed device to be detected and the object to be detected from being contaminated by the outside.
為達成上述目的,本發明提供一種微生物檢測系統,該微生物檢測系統係供設於一封閉式之待檢測裝置,其包括一流道及一檢測模組。該待檢測裝置內之一待檢測流體係供流通於該流道。該檢測模組設於該流道,包括二玻片、一顯微模組及至少一伸縮機構,該二玻片設於該流道之相對二側,至少一該玻片為可透光,該顯微模組設於可透光之該玻片遠離另一該玻片之一側,各該伸縮機構連接於一該玻片與該流道。其中,當該二玻片相互靠近貼合時,透過該顯微模組即可觀察到該二玻片之縫隙內的該待檢測流體。In order to achieve the above object, the present invention provides a microbial detection system for use in a closed device to be detected, which includes a first-class track and a detection module. One of the to-be-detected flow systems in the device to be tested is circulated to the flow channel. The detection module is disposed on the flow channel, and includes a two-slide, a micro-module and at least one telescopic mechanism. The two-slide is disposed on opposite sides of the flow channel, and at least one of the slides is permeable to light. The micro-module is disposed on a side of the slide that is permeable to light away from the other of the slides, and each of the telescopic mechanisms is coupled to the slide and the flow path. Wherein, when the two slides are close to each other, the fluid to be detected in the gap of the two slides can be observed through the micro-module.
以下僅以實施例說明本發明可能之實施態樣,然並非用以限制本發明所欲保護之範疇,合先敘明。The following is a description of the possible embodiments of the present invention, and is not intended to limit the scope of the invention as claimed.
請參考圖1至4,其顯示本發明之第一較佳實施例,本發明之微生物檢測系統係供設於一封閉式之待檢測裝置9,該微生物檢測系統包括一流道1及一檢測模組8。該待檢測裝置9係為一封閉式槽桶91(如煮鍋或細菌培養槽)。Referring to Figures 1 to 4, there is shown a first preferred embodiment of the present invention. The microorganism detecting system of the present invention is provided in a closed device to be detected 9, the microorganism detecting system comprising a first-class track 1 and a detecting mode. Group 8. The device to be tested 9 is a closed tank 91 (such as a skillet or a bacterial culture tank).
該待檢測裝置9內之一待檢測流體係供流通於該流道1。A flow system to be detected in the device to be detected 9 is circulated to the flow channel 1.
該檢測模組8設於該流道1,包括二玻片2、一顯微模組3及至少一伸縮機構7,該二玻片2設於該流道1之相對二側,至少一該玻片2為可透光,該顯微模組3設於可透光之該玻片2遠離另一該玻片2之一側,以具有足夠之光源觀察,各該伸縮機構7連接於一該玻片2與該流道1。The detecting module 8 is disposed on the flow channel 1 and includes a two-slide 2, a micro-module 3 and at least one telescopic mechanism 7. The two slides 2 are disposed on opposite sides of the flow channel 1, at least one of the two The slide 2 is permeable to light, and the micro-module 3 is disposed on the side of the slide 2 that is permeable to light away from the other of the slides 2, so as to have sufficient light source to observe, each of the telescopic mechanisms 7 is connected to one The slide 2 is connected to the flow path 1.
其中,當該二玻片2相互靠近貼合時,透過該顯微模組3即可觀察到該二玻片2之縫隙內的該待檢測流體,較佳地該顯微模組3包括一高倍率鏡組31,以使該顯微模組3可有效觀察到微生物。因此,無需進行將該待檢測裝置9打開連通外界及取出部分該待檢測流體,該微生物檢測系統即可準確地檢測出該待檢測裝置9之待檢測流體,並且可有效防止該待檢測裝置9內之待檢測流體連通外界而遭到外界之汙染。Wherein, when the two slides 2 are close to each other, the fluid to be detected in the gap of the two slides 2 can be observed through the micromodule 3, preferably the micromodule 3 includes a The high magnification lens set 31 is such that the microscopic module 3 can effectively observe microorganisms. Therefore, the fluid to be detected of the device to be detected 9 can be accurately detected by the microbial detection system, and the device to be detected can be effectively prevented. The fluid to be detected is connected to the outside and is contaminated by the outside.
於本實施例中部分該流道1係為一方形管路11,該二玻片2分別設於該方形管路11之相對二側,該伸縮機構7連接於該玻片2與該方形管路11之一側,於本實施例中該微生物檢測系統包括一該伸縮機構7,該伸縮機構7設於遠離該顯微模組3之一該玻片2,該較佳地該方形管路11設有該二玻片2之二側至少為可透光,以具有更足夠之光源進行檢測。值得一提的是,於本實施例中該伸縮機構7係為一蛇腹管體71,該蛇腹管體71係連接於該玻片2與該方形管路11之一側,可順利地移動該玻片2,且可有效封閉該方形管路11以防止該待檢測流體流出至外界。In the embodiment, the flow channel 1 is a square pipe 11 , and the two slides 2 are respectively disposed on opposite sides of the square pipe 11 , and the telescopic mechanism 7 is connected to the slide 2 and the square pipe. On one side of the road 11, in the embodiment, the microbial detection system comprises a telescopic mechanism 7, which is disposed away from the slide 2 of the micro-module 3, preferably the square tube 11 is provided with two sides of the two slides 2 at least permeable to light, with more sufficient light source for detection. It should be noted that in the embodiment, the telescopic mechanism 7 is a bellows body 71, and the bellows body 71 is connected to one side of the slide 2 and the square pipe 11 to smoothly move the The slide 2 can effectively close the square pipe 11 to prevent the fluid to be detected from flowing out to the outside.
較佳地,該微生物檢測系統可另包括至少一制動件4,該至少一制動件4至少設於一該玻片2,以制動該二玻片2相互靠近移動,具體而言,於本實施例中該檢測模組8包括一該制動件4,該制動件4係設於遠離該顯微模組3之一該玻片2,該制動件4係為可選擇性地伸縮之伸縮元件,以將遠離該顯微模組3之一該玻片2朝鄰近該顯微模組3之另一該玻片2移動或遠離,於其它實施例中可將該二玻片2皆設有一該制動件4(如圖5所示)亦無不可。當然,於其它實施例亦可不設有制動件,而以手動移動該二玻片亦可。Preferably, the microbial detection system may further comprise at least one brake member 4, and the at least one brake member 4 is disposed at least on the slide 2 to brake the two slides 2 to move closer to each other, in particular, in the present embodiment. In the example, the detecting module 8 includes a braking member 4 that is disposed away from the slide 2 of the micro-module 3, and the braking member 4 is a telescopic element that can be selectively telescoped. To move or move the slide 2 away from one of the micro-modules 3 toward the other slide 2 adjacent to the micro-module 3, in other embodiments, the two slides 2 can be provided with one. Brake 4 (shown in Figure 5) is also not available. Of course, in other embodiments, the brake member may not be provided, and the two slides may be manually moved.
值得一提的是,該微生物檢測系統可另包括一加壓裝置51,該加壓裝置51設於該流道1以供將該待檢測流體自該待檢測裝置9內經由該流道1之一端輸送至該二玻片2之間,該待檢測流體並經由該流道1之另一端輸送回該待檢測裝置9內,於本實施例中該加壓裝置51係為泵,且該加壓裝置51係以正壓方式將該待檢測流體輸送至該二玻片2之間,於其它實施例中當然亦可用負壓方式輸送,該加壓裝置51可使位於該待檢測裝置9內之待檢測流體順利地流至該流道1及該二玻片2之間以供該檢測模組8進行檢測,另外由於該加壓裝置51亦可將該待檢測流體經由該流道1之另一端輸送回該待檢測裝置9內,因此可確保該檢測模組8所檢測到之該待檢測流體係即時自該待檢測裝置9內所取得,以提升檢測之準確性。It is worth mentioning that the microbial detection system may further comprise a pressurizing device 51, wherein the pressurizing device 51 is disposed in the flow channel 1 for the fluid to be detected to pass through the flow channel 1 from the device to be detected 9 One end is transported between the two slides 2, and the fluid to be detected is transported back to the device to be detected 9 via the other end of the flow path 1. In the embodiment, the pressurizing device 51 is a pump, and the adding The pressure device 51 delivers the fluid to be detected to the two slides 2 in a positive pressure manner. In other embodiments, it can of course be transported in a negative pressure manner. The pressurizing device 51 can be located in the device 9 to be detected. The fluid to be detected flows smoothly between the flow channel 1 and the two slides 2 for detection by the detection module 8, and the pressure device 51 can also pass the fluid to be detected through the flow channel 1 The other end is sent back to the device 9 to be detected, so that the flow detection system detected by the detection module 8 is automatically acquired from the device to be detected 9 to improve the accuracy of the detection.
較佳地,該微生物檢測系統可另包括至少一發光元件52(於本實施例中係為LED燈,於其它實施例中亦可為其它種類之發光元件),各該發光元件52係設於一該玻片2遠離另一該玻片2之一側,以具有足夠之光線進行觀察,於本實施例中係為一該發光元件52,該發光元件52設於鄰近該顯微模組3之該玻片2的一側,於其它實施例中亦可於該二玻片2之一側皆設有一該發光元件52。Preferably, the microbial detection system may further comprise at least one light-emitting element 52 (in this embodiment, an LED lamp, and in other embodiments, other types of light-emitting elements), each of the light-emitting elements 52 is A light-emitting element 52 is disposed adjacent to the micro-module 3 in the present embodiment. One side of the slide 2 may be provided with one of the light-emitting elements 52 on one side of the two slides 2 in other embodiments.
如圖6所示,相較於上述第一實施例,於本發明之第二實施例中,微生物檢測系統另包括一影像擷取裝置61,該影像擷取裝置61係設於該顯微模組3,以供擷取及錄製該二玻片2之隙縫內所投影到該顯微模組3之一擷取影像,於本實施例中該影像擷取裝置61係為CCD (感光耦合元件)影像感測器,於其它實施例中亦為CMOS(互補性氧化金屬半導體)影像感測器亦無不可。As shown in FIG. 6, in the second embodiment of the present invention, the microbial detection system further includes an image capturing device 61, and the image capturing device 61 is disposed on the microscopic mode. The image capturing device 61 is a CCD (photosensitive coupling element) in the embodiment, for capturing and recording the image projected to the micro-module 3 in the slit of the two-slide 2 The image sensor is also a CMOS (Complementary Oxide Metallic Semiconductor) image sensor in other embodiments.
進一步的說,於本實施例中該微生物檢測系統另包括一顯示單元62及一雲端資料庫63,該顯示單元62係電性連接於該影像擷取裝置61,以將該擷取影像傳送至該顯示單元62進行播放;該雲端資料庫63係通訊連接該影像擷取裝置61,該影像擷取裝置61所擷取之該擷取影像係傳輸至該雲端資料庫63,因此使用者無需親自前往該顯微模組進行觀察檢測,透過觀察該顯示單元62所播放之該截取影像或是使用一裝置(如手機或電腦)連上該雲端資料庫63觀看該截取影像,即可得知該待檢測流體之狀況,故可有效增加檢設之便利性且可進行即時監控。於其它實施例中微生物檢測系統亦可依照實際使用需求而僅包括該顯示單元62及該雲端資料庫63其中之一即可,其餘結構與上述第一實施例相同,故不再多加敘述。Further, in the embodiment, the microbial detection system further includes a display unit 62 and a cloud database 63. The display unit 62 is electrically connected to the image capturing device 61 to transmit the captured image to The display unit 62 is configured to be played; the cloud database 63 is connected to the image capturing device 61, and the captured image captured by the image capturing device 61 is transmitted to the cloud database 63, so that the user does not need to personally Going to the micro-module for observation and observation, by observing the intercepted image played by the display unit 62 or using a device (such as a mobile phone or a computer) to connect to the cloud database 63 to view the captured image, the The condition of the fluid to be tested can effectively increase the convenience of the inspection and can be monitored in real time. In other embodiments, the microbial detection system may include only one of the display unit 62 and the cloud database 63 according to actual use requirements. The rest of the structure is the same as that of the first embodiment described above, and therefore will not be described again.
如圖7及圖8所示,相較於上述第一實施例,於本發明之第三實施例中,微生物檢測系統不包括加壓裝置而另包括一活塞裝置53,該活塞裝置53係設於流道1A相對該待檢測裝置9之一端,該檢測模組8係位於該活塞裝置53與該待檢測裝置9之間,該活塞裝置53係供將該待檢測流體自該待檢測裝置9內經由該流道1A輸送至該檢測模組8,該活塞裝置53可另供將該待檢測流體輸送回該待檢測裝置9內,因此於第三實施例同樣可確保該檢測模組8所檢測到之該待檢測流體係自該待檢測裝置9內所即時取得,以提升檢測之準確性,其餘結構與上述第一實施例相同,故不再多加敘述。As shown in FIG. 7 and FIG. 8, in the third embodiment of the present invention, the microorganism detecting system does not include a pressurizing device and further includes a piston device 53, which is provided in the third embodiment. The detecting module 8 is located between the piston device 53 and the device to be detected 9 , and the piston device 53 is for supplying the fluid to be detected from the device 9 to be detected. The detection device 8 is ensured to be transported to the detection module 8 via the flow channel 1A. The piston device 53 can additionally supply the fluid to be detected back to the device to be detected 9 , so that the detection module 8 can be ensured in the third embodiment. The flow system to be detected is detected from the device to be detected 9 to improve the accuracy of the detection. The rest of the structure is the same as that of the first embodiment described above, and therefore will not be described again.
要說明的是,如圖9所示,相較於上述第一實施例,於本發明第四實施例中,微生物檢測系統亦可應用於為一封閉式管道(如液體輸送管道)92之待檢測裝置9A,該加壓裝置51同樣地可使位於該待檢測裝置9A內之待檢測流體順利地流至該流道1及該檢測模組8以供該檢測模組8進行檢測,以及將該待檢測流體經由該流道1之另一端輸送回該待檢測裝置9A內,其餘結構與上述第一實施例相同,故不再多加敘述。可理解的是,如圖10所示,相較於上述第四實施例,本發明第五實施例之微生物檢測系統亦可不包括加壓裝置而另包括該活塞裝置53,該活塞裝置53係同樣地可供將該待檢測流體自該待檢測裝置9A內經由該流道1A輸送至該檢測模組8,另供將該待檢測流體輸送回該待檢測裝置9A內,其餘結構與上述第四實施例相同,故不再多加敘述。It should be noted that, as shown in FIG. 9, in the fourth embodiment of the present invention, the microorganism detecting system can also be applied to a closed pipe (such as a liquid conveying pipe) 92 as compared with the first embodiment. The detecting device 9A, the pressing device 51 can smoothly flow the fluid to be detected located in the device to be detected 9A to the flow channel 1 and the detecting module 8 for detection by the detecting module 8, and The fluid to be detected is transported back to the device to be detected 9A via the other end of the flow path 1. The rest of the structure is the same as that of the first embodiment described above, and therefore will not be described again. It can be understood that, as shown in FIG. 10, the microorganism detecting system according to the fifth embodiment of the present invention may further include the pressing device instead of the pressing device, and the piston device 53 is the same as the fourth embodiment. The fluid to be detected is transported from the inside of the device to be detected 9A to the detection module 8 via the flow channel 1A, and the fluid to be detected is transported back to the device to be detected 9A, and the remaining structure and the fourth The embodiments are the same, so the description will not be repeated.
綜上,本發明微之生物檢測系統無需進行將該待檢測裝置打開連通外界及取出部分該待檢測流體,該微生物檢測系統即可準確地檢測出該待檢測裝置之待檢測流體,並且可有效防止該待檢測裝置內之待檢測流體連通外界而遭到外界之汙染。In summary, the micro-biological detection system of the present invention does not need to open the device to be detected to communicate with the outside and extract a portion of the fluid to be detected, and the microbial detection system can accurately detect the fluid to be detected of the device to be detected, and can be effective. The fluid to be detected in the device to be detected is prevented from being connected to the outside and is contaminated by the outside.
並且,使用者無需親自前往該檢測模組及該待檢測裝置進行觀察檢測,透過觀察該顯示單元所播放之該截取影像或是使用一裝置連上該雲端資料庫觀看,即可得知該待檢測流體之狀況,故可達到即時監控之效果。Moreover, the user does not need to go to the detection module and the to-be-detected device to perform observation and detection, and observe the intercepted image played by the display unit or use a device to connect to the cloud database to view the waiting for the image. The condition of the fluid is detected, so that the effect of immediate monitoring can be achieved.
1,1A‧‧‧流道
2‧‧‧玻片
3‧‧‧顯微模組
4‧‧‧制動件
7‧‧‧伸縮機構
8‧‧‧檢測模組
9,9A‧‧‧待檢測裝置
11‧‧‧方形管路
31‧‧‧高倍率鏡組
51‧‧‧加壓裝置
52‧‧‧發光元件
53‧‧‧活塞裝置
61‧‧‧影像擷取裝置
62‧‧‧顯示單元
63‧‧‧雲端資料庫
71‧‧‧蛇腹管體
91‧‧‧槽桶
92‧‧‧管道1,1A‧‧‧ runner
2‧‧‧ slides
3‧‧‧Micromodule
4‧‧‧Brakes
7‧‧‧Flexing mechanism
8‧‧‧Test module
9,9A‧‧‧Device to be tested
11‧‧‧square pipe
31‧‧‧High magnification mirror
51‧‧‧ Pressurizing device
52‧‧‧Lighting elements
53‧‧‧ piston device
61‧‧‧Image capture device
62‧‧‧Display unit
63‧‧‧Cloud database
71‧‧‧The belly tube
91‧‧‧ barrel
92‧‧‧ Pipes
圖1為本發明第一較佳實施例之立體圖。 圖2為本發明第一較佳實施例之結構示意圖。 圖3及圖4為本發明第一較佳實施例之操作示意圖。 圖5為本發明第一較佳實施例之另一態樣操作示意圖。 圖6為本發明第二較佳實施例之操作示意圖。 圖7及圖8為本發明第三較佳實施例之結構示意圖。 圖9為本發明第四較佳實施例之操作示意圖。 圖10為本發明第五較佳實施例之操作示意圖。1 is a perspective view of a first preferred embodiment of the present invention. 2 is a schematic structural view of a first preferred embodiment of the present invention. 3 and 4 are schematic views showing the operation of the first preferred embodiment of the present invention. FIG. 5 is a schematic diagram of another aspect of operation of the first preferred embodiment of the present invention. Figure 6 is a schematic view showing the operation of a second preferred embodiment of the present invention. 7 and 8 are schematic views showing the structure of a third preferred embodiment of the present invention. Figure 9 is a schematic view showing the operation of a fourth preferred embodiment of the present invention. Figure 10 is a schematic view showing the operation of the fifth preferred embodiment of the present invention.
1‧‧‧流道 1‧‧‧ flow path
51‧‧‧加壓裝置 51‧‧‧ Pressurizing device
8‧‧‧檢測模組 8‧‧‧Test module
91‧‧‧槽桶 91‧‧‧ barrel
9‧‧‧待檢測裝置 9‧‧‧Device to be tested
Claims (10)
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TW105114391A TWI592492B (en) | 2016-05-10 | 2016-05-10 | Microbial Detecting System |
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