TWI548456B - A centrifugal consumable, system and its separation method - Google Patents

Description

Centrifugal consumables, system and separation method thereof

The present invention relates to a separation method, a centrifugal system and a centrifugal consumable, in particular to a consumable, method and system for separating a solution in a sealed environment, ensuring that the components of the separation solution are pure.

Today's centrifuges have been widely used in various fields, such as the chemical field and the medical field. The principle is to use the powerful centrifugal force generated by the high-speed rotation of the centrifuge rotor, and use the centrifugal force to accelerate the sedimentation speed of the particles in the solution, and separate the sedimentation coefficient and the mass of the mass in the solution, wherein the formula of the centripetal acceleration is known. The object with a higher density is below, and the object with a lower density is above.

In the medical field, the conventional blood centrifugation system is used to separate various components in the blood, such as high-concentration platelet plasma and platelet-poor plasma, which is gradually receiving attention from the medical community and the medical beauty industry. High-concentration platelet plasma (Platelet Rich Plasma) , PRP) contains high concentrations of growth factors, such as: PDGF aa, PDGF bb, PDGF ab, IGF-I, IGF-II, TGF-alpha, TGF-beta, EGF, VEGF, can treat tendonitis, acute and chronic muscles Pulling, ligament sprain, loss of articular cartilage, rheumatoid arthritis, etc., can also be used to inject into the skin, repair tissue, cell activation, improve pigmentation, acne scars, wounds, and cavities. Platelet Poor Plasma (PPP) can be used in filling technology to make small platelet plasma into autologous collagen filling and implant. Under the skin, it can immediately improve wrinkles, legal lines, depressions, etc., and the effect can last for three to four months. Therefore, it is possible to isolate high-concentration platelet plasma and platelet-poor plasma with high economic value.

However, the conventional technique is to house the blood in a centrifuge tube. After the centrifugation is completed, the operator inserts the syringe into the centrifuge tube, and the collection form is manually collected, and the extraction is more likely to occur during the extraction process. Red blood cells, and the plasma collected by such separation method cannot directly collect high-concentration platelet plasma and platelet-poor plasma. After the collection, the platelet plasma must be separated from the high-concentration platelet plasma. Further, the volume of the centrifuge tube is small, each time. The centrifugation requires collection of plasma in a plurality of centrifuge tubes, which is cumbersome and complicated. Therefore, the conventional blood centrifugation system cannot be directly used after the collection is completed, and must be extracted through the syringe in the collected sample before being injected and used, and when extracted, it is easy to cause the extraction component to be affected due to inadvertent operation. Pollution.

For example, U.S. Patent Publication No. 5,514,070 A discloses a centrifugal device for injecting a solution to be separated into a device via a line, and rotating the solution to be separated at a high speed. When the solution to be separated is separated, The continuous separation of the solution to be separated increases the pressure, and the separated solution to be separated is forced out by another connected line, and the separated separation solution to be separated is extracted by the external transfer tube and the pressure valve. come out.

Among them, such technology implies several problems, one of which is that the structure of the centrifugal device includes a stator and a rotor, and both of them must generate friction when rotating at a high speed, and the user cannot ensure the presence or absence of impurities under high-speed friction. The second is that the external pipeline is equipped with a pressure valve so that the separated solution to be separated is not closed during the entire separation process, and it is not known whether it is possible to leak impurities from the pressure valve, so the rigor of the overall separation step is not known. Tested.

Because of the indispensable drawbacks of the prior art, the present invention proposes a new centrifugal consumable, a new separation system, and a new separation method by which the deficiencies of the prior art can be overcome.

The invention firstly provides a centrifugal consumable for separating materials of different sedimentation coefficients and density qualities in a solution, the centrifugal consumable comprising the following structure: a first centrifugal power connection structure, the first centrifugal power connection structure is used In order to allow the centrifugal consumable to receive an external force, thereby performing a rotation of the solution at a predetermined rotational speed; a first space, the first space is used to store the solution to be separated, and the first space has an injection portion, The solution enters the first space through the injection portion, and the centrifugal charge is rotated at a high speed by the first centrifugal power connection structure to separate the solution, and the solution separates and generates a first primary separation solution and a second primary separation solution. Wherein the first primary separation solution and the second primary separation solution have a boundary point; a second space, a first transmission path between the second space and the first space, and the second being squeezed The first separation solution can be diffused into the second space through the first transmission channel; a pressing portion slidably correspondingly disposed on the first portion Between the space and the second space, the first space and the volume of the second space are changed by the sliding portion to further compress the first space, so that the second primary separation solution is squeezed from the first space a space is diffused to the second space; a third space is disposed on the outer side of the centrifugal consumable relative to the second space, and is coupled to the second space through a second transmission channel, the second initial The separation solution may continue to be centrifugally separated to produce a first secondary separation solution and a second secondary separation solution, wherein the second secondary separation solution diffuses toward the third space located at the outer edge due to centrifugal force; The injection portion injects the solution into the first space and utilizes the first After the centrifugal power connection structure performs the rotation of the predetermined rotation speed, the solution is separated into the first primary separation solution and the second primary separation solution, and then the first space is forced through the pressing portion to force the second primary separation solution to pass through. The first transfer channel enters the second space, and then the second primary separation solution is continuously separated into the first secondary separation solution and the second secondary separation solution, and the second secondary separation solution passes through the first The second transmission path is separated to the third space on the outer side to complete the separation.

Preferably, the pressing portion comprises a buffer structure, a pressing structure and an isolation structure. And the buffer structure is preferably a spring.

Preferably, the pressing portion and the first space and the second space further comprise a first leak stop structure and a second leak stop structure.

The centrifugal consumable described above is a consumable, meaning that it must be discarded when it is used up. It is mentioned in the above that the centrifugal consumable is driven by an external force to rotate, and therefore a system including a rotary power is proposed here. The present invention next provides a centrifugal system for separating materials of different sedimentation coefficients and density qualities in a solution. The centrifugal system comprises the following structure: a centrifugal consumable comprising: a first centrifugal power connection structure The first centrifugal power connection structure is configured to enable the centrifugal consumable to receive an external force, thereby performing a rotation of the solution at a predetermined rotational speed; and a first space for storing the solution to be separated, And the first space has an injection portion, the solution enters the first space by the injection portion, and the centrifugal power supply is rotated at a high speed by the first centrifugal power connection structure to separate the solution, and the solution is separated to generate a first time. a separation solution and a second primary separation solution, wherein the first primary separation solution and the second primary separation solution have a boundary point; a second space, a first space between the second space and the first space Transport path, the second primary separation solution that is squeezed The first transmission channel can be diffused to the second space; a pressing portion slidably correspondingly disposed between the first space and the second space, and the sliding portion is changed by sliding The first space and the volume of the second space further compress the first space, so that the second primary separation solution is diffused from the first space to the second space by being squeezed; a third space, the third space The second primary separation solution can be continuously centrifuged to generate a first secondary separation solution and a second portion. The second primary separation solution can be continuously centrifuged to be separated from the second storage space. a second separation solution, wherein the second secondary separation solution diffuses toward the third space at the outer edge due to centrifugal force; and an inductor, the inductor is disposed correspondingly to the first transmission channel, the induction The device is configured to detect the position of the demarcation point; a centrifugal power mechanism that carries the centrifugal consumable and gives the centrifugal consumable power to perform a centrifugal rotation action, the centrifugal power mechanism The load bearing system has a second centrifugal power connection structure, and one end of the second centrifugal power connection structure is coupled to the first centrifugal power connection structure; And a carrying base for supporting the carrying body, the carrying base comprising a third centrifugal power connecting structure and a motor, wherein the other end of the second centrifugal power connecting structure is connected with the third centrifugal power Structure, the motor can drive the centrifugal consumable to rotate.

Wherein, the solution is injected into the first space from the injection portion, and after the rotation of the predetermined rotational speed is performed by the first centrifugal power connection structure, the solution is separated into the first primary separation solution and the second primary separation solution, and then Squeezing the first space through the pressing portion to force the second primary separation solution to enter the second space through the first transmission channel, and sensing the boundary point using the inductor to prevent the first primary separation solution from entering The second space, and then the second primary separation solution is continuously separated into the first secondary separation solution and the second secondary separation solution, The second secondary separation solution is separated by centrifugal force through the second transmission path to the third space on the outer side to complete the separation.

Preferably, the pressing portion comprises a buffer structure, a pressing structure and an isolation structure.

Based on the above-mentioned centrifugal consumables and centrifugal system as a tool, the present invention proposes a separation method for separating substances of different sedimentation coefficients and density qualities in a solution, the method comprising the following steps: an injection step, the injection The step of injecting a solution into a first space in a centrifugal consumable through an injection portion, wherein the centrifugal consumable further comprises a second space and a third space; and a centrifugal separation starting step, the centrifugal separation starting step Separating the centrifugal consumables at a predetermined rotation speed with a suitable rotation center, separating the sedimentation coefficient and the density mass of the solution by a predetermined rotation speed, wherein the solution in the first space is separated by a predetermined rotation speed a first primary separation solution and a second primary separation solution, and the first primary separation solution and the second primary separation solution have a boundary point; and a pressurized separation step, the pressurized separation step is predetermined The first space is compressed by a pressing portion during high-speed rotation of the rotating speed, so that the second initial in the first space The separation solution is squeezed to enter the second space through a first transmission path, and the boundary point simultaneously moves toward the second space along with the second primary separation solution, and has an inductor, the induction Corresponding to the first transport lane, the position of the demarcation point is sensed to prevent the first primary separation solution from entering the second space; and a second phase separation step is provided in the second phase separation step The second primary separation solution of the second space continues to rotate at a high speed at a predetermined rotational speed, and the second primary separation solution is separated into a first secondary separation solution and a second secondary separation solution, and the second second The secondary separation solution is separated into the first passage through a second transmission passage due to centrifugal force Three spaces.

The pressing portion is slidably disposed correspondingly between the first space and the second space, and the volume of the first space and the second space can be changed by sliding and sliding the pressing portion.

Preferably, the injection portion is a recoverable material, and the injection portion is automatically sutured after the solution is injected through the injection portion to prevent the solution from flowing backward.

Preferably, a first leak-stop structure and a second leak-stop structure are disposed between the pressing portion and the first space and the second space. Moreover, wherein the first leak-stop structure and the second leak-stop structure are O-rings are preferred.

Preferably, the sensor is an optical sensor, and the centrifugal consumable is made of a transparent material, and the optical sensor can distinguish the position of the boundary point by the degree of transmission and/or reflection of light.

Wherein, the solution is finally separated into the first primary separation solution, the first secondary separation solution, and the second secondary separation solution, and the first primary separation solution is located in the first space, the first secondary separation The solution is located in the second space, and the second secondary separation solution is located in the third space. When the separation of the solution is completed, the centrifugal consumable is stopped to rotate to open the third space to take out the second secondary separation solution.

Even by using a plurality of the centrifugal consumables, the cycle of the separation method can be performed. When the cycle of the separation method is performed, the third space of one of the centrifugal consumables is connected to the first space of another centrifugal consumable. In the second stage of the separation step, the injection step is restarted to achieve a cycle whereby the solution can be easily separated to be more pure.

The above is only a brief description of each step, and the details of each specific embodiment and implementation steps will advance. One step is revealed as follows.

100‧‧‧ Centrifugal system

1‧‧‧ Centrifugal consumables

11‧‧‧solution

111‧‧‧First primary separation solution

1111‧‧‧demarcation point

112‧‧‧Second initial separation solution

113‧‧‧Second separation solution

114‧‧‧Second separation solution

12‧‧‧First centrifugal power connection structure

13‧‧‧First space

131‧‧‧First transmission lane

132‧‧‧First leak-stop structure

133‧‧‧Injection Department

14‧‧‧Second space

141‧‧‧second transmission lane

142‧‧‧second leak-stop structure

15‧‧‧Extrusion Department

151‧‧‧ Buffer structure

152‧‧‧ Pressure structure

153‧‧‧Isolation structure

16‧‧‧ Third Space

17‧‧‧ sensor

2‧‧‧ centrifugal power mechanism

21‧‧‧ Carrying ontology

211‧‧‧Second centrifugal power connection structure

22‧‧‧Loading base

221‧‧‧ Third centrifugal power connection structure

222‧‧‧ motor

200‧‧‧Separation method

S1‧‧‧ injection step

S2‧‧‧ Centrifugal separation start-up steps

S3‧‧‧Pressure separation step

S4‧‧‧Second stage separation step

The first diagram is an embodiment showing the separation system; the second diagram A and the second diagram B are examples showing the centrifugal consumable; the third diagram is an embodiment showing the carrier body; the fourth diagram is An embodiment of the carrier base is shown; the fifth and fifth panels are examples showing the centrifugal consumable; and the sixth is a step flow showing the separation method.

Example of a separation system

Please refer to the first to fifth B drawings, which are examples showing the separation system.

In order to overcome the deficiencies of the centrifugal system and the device in the prior art, the present invention first proposes a centrifugal consumable 1 which separates different sedimentation coefficients and density mass substances in a solution by rotating the centrifugal consumable 1 at a high speed. In this embodiment, the centrifugal consumable 1 is a disposable consumable, and the user needs to replace the new centrifugal consumable 1 after the use to perform the next separation operation. The centrifugal consumable 1 includes the following structures: a first centrifugal power connection structure 12, a first space 13, a second space 14, a pressing portion 15, and a third space 16. The first centrifugal power supply structure 12 is used to drive the whole centrifugal consumable 1 , and the solution 11 stored in the first space 13 is rotated at a high speed into the first primary separation solution 111 and the second primary separation solution 112 . Compressing the first portion by the pressing portion 15 A space 13 forces the inner second primary separation solution 112 into the second space 14, and further separates the second primary separation solution 112 into a first secondary separation solution 113 and a second secondary separation solution 114 by high-speed rotation. After the second separation solution 114 is separated, it enters the third space 16 to complete the separation. When the second primary separation solution 112 is pressed by the pressing portion 15, the boundary point 1111 between the first primary separation solution 111 and the second primary separation solution 112 is sensed by the inductor 17 to prevent the first primary The separation solution 111 flows into the second space 14. A more detailed description of each configuration and its functions is provided below.

Referring to FIG. 2B, regarding the first centrifugal power connection structure 12 in this embodiment, the first centrifugal power connection structure 12 is used to allow the centrifugal consumable 1 to receive an external force, thereby performing a rotational separation of a solution at a predetermined rotational speed. In the action of 11, the predetermined rotational speed is related to the material component to be separated, which is common knowledge of those skilled in the art, so the centrifugal consumable 1 is combined with the external force device by using the first centrifugal power connecting structure 12. In turn, the overall rotation is driven, and the structure of the device for providing the external force will be described in more detail later.

Regarding the first space 13 in this embodiment, the first space 13 is used to store the solution 11 to be separated, and the first space 13 has an injection portion 133, and the solution 11 enters the injection portion 133. The first space 13 is driven by the first centrifugal power connection structure 12 to rotate the centrifugal consumable 1 at a high speed and separate the solution 11. The solution 11 separates and generates a first primary separation solution 111 and a second primary separation solution 112. The first primary separation solution 111 and the second primary separation solution 112 have a boundary point 1111 therein. Preferably, the injection portion 133 is made of a recoverable material. After the solution 11 is injected through the injection portion 133, the injection portion is automatically sutured to prevent the solution 11 from flowing backward.

The second primary separation solution 112 in the first space 13 passes through the extrusion portion 15 The pressure flows into the second space 14 through a first transmission path 131. Please refer to FIG. 5A and FIG. 5B, wherein the pressing portion 15 is slidably disposed correspondingly between the first space 13 and the second space 14, and the pressing portion 15 is slid to change the first portion. A space 13 and a volume of the second space 14 further compress the first space 13 such that the second primary separation solution 112 is diffused from the first space 13 to the second space 14 by being squeezed. Preferably, the pressing portion 15 and the first space 13 and the second space 14 further comprise a first leakage structure 132 and a second leakage structure 142, and the first stop The leakage structure 132 and the second leakage stop structure 142 are preferably O-rings to prevent the solution 11 from being exposed. The pressing portion 15 includes a buffering structure 151, a pressing structure 152 and an isolating structure 153, and the buffering structure 151 is preferably a spring. The pressing structure 152 is used for pushing the pressing portion 15 by force. The isolation structure 153 is used to isolate the first space 13 and the second space 14.

Next, the second primary separation solution 112 is separated by a predetermined number of rotations, and the second primary separation solution 112 is separated into the first secondary separation solution 113 and the second secondary separation solution 114. The second secondary separation solution 114 enters a third space 16 through a second transmission path 141 due to the centrifugal force. The third space 16 is disposed on the outer side of the centrifugal consumable 1 with respect to the second space 14.

The centrifugal consumable 1 described above is a consumable, meaning that it must be discarded when it is used up. It is mentioned in the above that the centrifugal consumable 1 is driven by an external force, and therefore a system including a rotary power is proposed here. The present invention next provides a centrifugal system 100 for separating a sedimentation coefficient and a density mass of a solution 1 comprising a centrifugal consumable 1 and a centrifugal power mechanism 2 with respect to the centrifugal consumable 1 The composition is the same as in the foregoing embodiment, and thus is not described herein. However, a sensor 17 is added, please refer to the first figure, wherein the sensor 17 is matched with the first transmission channel 131, and the sensor 17 is used for detecting the sensing. The position of the boundary point 1111 prevents the first primary separation solution 111 from entering the second space 14, maintaining the purity of the second primary separation solution 112. In particular, in this embodiment, the inductor is an optical sensor, and the material of the centrifugal consumable 1 is transparent at this time, so that the position of the demarcation point can be distinguished by the degree of transmission and/or reflection of light by the optical sensor.

In the centrifugal power mechanism 2, the centrifugal power mechanism 2 carries the centrifugal consumable 1 and gives a centrifugal rotation to the centrifugal consumable 1 . The centrifugal power mechanism 2 includes a carrier body 21 and a carrier base 22 .

The carrier body 21 is configured to carry the centrifugal consumable 1 . The carrier body 21 has a second centrifugal power connection structure 211 , and one end of the second centrifugal power connection structure 211 is coupled to the first centrifugal power connection structure 12 . Linked to provide the power of the centrifugal consumable 1.

The carrying base 22 is configured to carry the carrying body 21, the carrying base includes a third centrifugal power connecting structure 221 and a motor 222, and the other end of the second centrifugal power connecting structure 211 and the third centrifugal The power connecting structure 221 is configured to drive the centrifugal consumable 1 to rotate.

The solution 1 is injected into the first space 13 from the injection portion 133, and after the rotation of the predetermined rotation speed is performed by the first centrifugal power connection structure 12, the solution 1 is separated into the first primary separation solution 111 and the The second primary separation solution 112, and then the first space 13 is forced through the pressing portion 15 to force the second primary separation solution 112 to enter the second space 14 through the first transmission path 131, and the inductor 17 is used. The demarcation point 1111 is sensed to prevent the first primary separation solution 111 from entering the second space 14. Then, the second primary separation solution 112 is continuously separated into the first secondary separation solution 113 and the second secondary separation solution 114, and the second secondary separation solution 114 is separated to the outside by the centrifugal force through the second transmission channel 141. This third space 16 in turn completes the separation.

The centrifugal system 100 in this embodiment successfully achieves a closed liquid separation and does not require the use of a pressure valve of the prior art, so that the present invention ensures that the level of purity of the material to be separated is superior to the prior art.

Example of separation method

Please refer to the first to sixth figures, which are examples showing the separation system and method.

Based on the above-mentioned centrifugal consumables and centrifugal system as a tool, the present invention proposes a separation method 200 for separating substances of different sedimentation coefficients and density qualities in the solution 11, please refer to the sixth figure, which includes the following Step: an injection step S1, a centrifugal separation initiation step S2, a pressure separation step S3, and a sensing step S4.

In the injection step S1, a solution 11 is injected into a first space 13 in a centrifugal consumable 1 through an injection portion 133, wherein the centrifugal consumable further comprises a second space 14 and a third space 16, The second space 14 and the third space 16 are connected by a second transmission path 141. Preferably, the injection portion 133 is made of a recoverable material. After the solution 11 is injected through the injection portion 133, the injection portion is automatically sutured to prevent the solution 11 from flowing backward.

Then, a centrifugal separation starting step S2 is performed, wherein the centrifugal separation starting step S2 is to continuously rotate the centrifugal consumable 1 at a predetermined rotation speed with a suitable rotation center, and the different sedimentation coefficients of the solution 11 are separated by the predetermined rotation speed. a density mass material, wherein the solution 11 in the first space 13 is separated into a first primary separation solution 111 and a second primary separation solution 112 by using the predetermined rotation speed, and the first primary separation solution 111 and the The second primary separation solution 112 has a boundary point 1111 therebetween.

Next, a pressure separation step S3 is entered, and the pressure separation step S3 is During the high-speed rotation of the predetermined rotation speed, the first space 13 is compressed by a pressing portion 15, so that the second primary separation solution 112 in the first space 13 is squeezed to enter the second through the first transmission path 131. At the same time, the boundary point 1111 simultaneously moves toward the second space 14 along with the second primary separation solution 112. There is a sensor 17, and the sensor 17 cooperates with the first transmission path 131 to sense the position of the boundary point 1111 to prevent the first primary separation solution 111 from entering the second space 14. Please refer to FIG. 5A and FIG. 5B, wherein the pressing portion 15 is slidably disposed correspondingly between the first space 13 and the second space 14, and the pressing portion 15 is slid to change the first portion. A space 13 and a volume of the second space 14 further compress the first space 13 such that the second primary separation solution 112 is diffused from the first space 13 to the second space 14 by being squeezed. Preferably, the pressing portion 15 and the first space 13 and the second space 14 further comprise a first leakage structure 132 and a second leakage structure 142, and the first stop The leakage structure 132 and the second leakage stop structure 142 are preferably O-rings to prevent the solution 11 from being exposed. The pressing portion 15 includes a buffering structure 151, a pressing structure 152 and an isolating structure 153, and the buffering structure 151 is preferably a spring. The pressing structure 152 is used for pushing the pressing portion 15 by force. The isolation structure 153 is used to isolate the first space 13 and the second space 14.

Then, a second stage separation step S4 is entered, in which the second primary separation solution 112 in the second space 14 continues to rotate at a high speed at the predetermined rotation speed in the second stage separation step S4, the second primary separation The solution 112 is separated into a first secondary separation solution 113 and a second secondary separation solution 114. Further, the second secondary separation solution 114 is separated into the third space 16 by a second transmission passage 141 due to centrifugal force. .

Wherein, the solution 11 is finally separated into the first primary separation solution 111, the first secondary separation solution 113, and the second secondary separation solution 114, and the first primary separation solution The 111 is located in the first space 13 , the first secondary separation solution 113 is located in the second space 14 , and the second secondary separation solution 114 is located in the third space 16 . When the separation of the solution 11 is completed, the centrifugal consumable 1 is stopped to rotate to open the third space 16 and the second secondary separation solution is taken out 114 to complete the separation.

Here, the circulation of the separation method 200 can be performed by using a plurality of the centrifugal consumables. When the cycle of the separation method 200 is performed, the third space 16 of one of the centrifugal consumables 1 is connected to the first space 13 of the other centrifugal consumable 1 before the injection step can be restarted in the second stage separation step S4. S1 achieves a cycle by which solution 11 can be easily separated to be more pure.

The separation method 200 in this embodiment successfully achieves a closed liquid separation and does not require the use of a pressure valve of the prior art, so that the present invention ensures that the purity of the substance to be separated is superior to the prior art.

Based on the above embodiments, the separation method and system provided by the present invention have industrial use value, but the above description is only for the preferred embodiment of the present invention, and those skilled in the art can Other modifications are possible in the description, but such modifications are still within the spirit of the invention and the scope of the invention as defined below.

100‧‧‧ Centrifugal system

1‧‧‧ Centrifugal consumables

17‧‧‧ sensor

2‧‧‧ centrifugal power mechanism

21‧‧‧ Carrying ontology

22‧‧‧Loading base

Claims (10)

A centrifugal consumable for separating a substance having different sedimentation coefficient and density mass in a solution, the centrifugal consumable comprising the following structure: a first centrifugal power connection structure, the first centrifugal power connection structure is used for the centrifugation The consumable material is capable of receiving an external force, thereby performing a rotation of the predetermined speed to separate the solution; a first space, the first space is for storing the solution to be separated, and the first space has an injection portion, the solution is The injection portion enters the first space, and the centrifugal power supply structure drives the centrifugal consumable to rotate at a high speed and separates the solution, and the solution separates and generates a first primary separation solution and a second primary separation solution, wherein the first separation solution a first separation solution and the second primary separation solution have a boundary point; a second space, a first transmission channel between the second space and the first space, the second primary separation solution being squeezed Dispersing through the first transmission path to the second space; a pressing portion slidably correspondingly disposed in the first space and the first portion Between the spaces, the first space and the volume of the second space are changed by sliding of the pressing portion, and the first space is further compressed, so that the second primary separation solution is diffused from the first space to the a second space; a third space, the third space is disposed on the outer side of the centrifugal consumable relative to the second space, and is coupled to the second space through a second transport channel, and the second primary separation solution can continue to be centrifuged Separating to generate a first secondary separation solution and a second secondary separation solution, wherein the second secondary separation solution diffuses toward the third space located at the outer edge due to centrifugal force; and wherein, from the injection portion Injecting the solution into the first space and utilizing the first centrifugal power connection After the knot structure performs the rotation of the predetermined rotation speed, the solution is separated into the first primary separation solution and the second primary separation solution, and then the first space is forced through the pressing portion to force the second primary separation solution to pass through the first portion. a transport path enters the second space, and then the second primary separation solution is continuously separated into the first secondary separation solution and the second secondary separation solution, and the second secondary separation solution passes through the second transmission due to centrifugal force The passage is separated to the third space on the outside to complete the separation. The centrifugal consumable of claim 1, wherein the pressing portion comprises a buffer structure, a pressing structure, and an isolation structure. The centrifugal consumable of claim 1, wherein the pressing portion and the first space and the second space further comprise a first leak stop structure and a second leak stop structure. A centrifugal system for separating materials of different sedimentation coefficients and density qualities in a solution, the centrifugal system comprising the following structure: a centrifugal consumable comprising: a first centrifugal power connection structure, the first centrifugation The power connection structure is configured to enable the centrifugal consumable to receive an external force, thereby performing a rotation of the solution at a predetermined rotational speed; a first space, the first space is used to store the solution to be separated, and the first space Having an injection portion, the solution enters the first space by the injection portion, drives the centrifugal consumable to rotate at a high speed through the first centrifugal power connection structure, and separates the solution, and the solution separates to generate a first primary separation solution and a first a second separation solution, wherein the first primary separation solution and the second primary separation solution have a boundary point; a second space, a first transmission path between the second space and the first space, the second primary separation solution being squeezed can be diffused into the second space through the first transmission channel; a pressing portion The pressing portion is slidably disposed between the first space and the second space, and the first space and the volume of the second space are changed by the sliding portion to further compress the first space. Dispersing the second primary separation solution from the first space to the second space by being squeezed; a third space, the third space is disposed on the outer side of the centrifugal consumable relative to the second space, and The second transfer channel is coupled to the second space, and the second primary separation solution can be further centrifuged to generate a first secondary separation solution and a second secondary separation solution, wherein the second secondary separation solution is due to centrifugal force Dividing toward the third space located at the outer edge; a centrifugal power mechanism carrying the centrifugal consumable and giving the centrifugal consumable power a centrifugal rotation action, the centrifugal power mechanism comprising: a bearing a body for carrying the centrifugal consumable, the carrier body has a second centrifugal power connection structure, one end of the second centrifugal power connection structure is coupled with the first centrifugal power connection structure; and a carrier base The carrying base is configured to carry the carrying body, the carrying base includes a third centrifugal power connecting structure and a motor, and the other end of the second centrifugal power connecting structure and the third centrifugal power connecting structure, the motor The centrifugal consumable is rotated, and an inductor is disposed corresponding to the first transmission path, and the inductor is configured a position for detecting the demarcation point; wherein the solution is injected into the first space from the injection portion, and after the rotation of the predetermined rotation speed is performed by the first centrifugal power connection structure, the solution is separated into the first first time Separating the solution and the second primary separation solution, and then pressing the first space through the pressing portion to force the second primary separation solution to enter the second space through the first transmission channel, and sensing the same using the inductor a dividing point to prevent the first primary separation solution from entering the second space, and then, the second primary separation solution is continuously separated into the first secondary separation solution and the second secondary separation solution, the second secondary separation solution The separation is completed by the centrifugal force passing through the second transmission path to the third space on the outer side. The centrifugal system of claim 4, wherein the pressing portion comprises a buffer structure, a pressing structure, and an isolation structure. A separation method for separating substances having different sedimentation coefficients and density qualities in a solution, the method comprising the following steps: an injection step of injecting a solution into a centrifugal consumable through an injection portion a first space, wherein the centrifugal consumable further comprises a second space and a third space; a centrifugal separation starting step, wherein the centrifugal separation starting step is to continuously rotate the centrifugal consumable at a predetermined rotation speed as a center of rotation Separating the different sedimentation coefficient and density mass of the solution by a predetermined rotation speed, wherein the solution in the first space is separated into a first primary separation solution and a second primary separation solution by using a predetermined rotation speed, and the a first separation solution and a second separation solution having a boundary point; and a pressure separation step of compressing the first space by a pressing portion during high-speed rotation at a predetermined rotation speed the solution of the second primary isolates the first space is squeezed into the beginning of the second air through a first transmission path At the same time, the demarcation point simultaneously moves toward the second space along with the second primary separation solution, and has a sensor, and the sensor cooperates with the first transmission channel to sense the position of the boundary point. Preventing the first primary separation solution from entering the second space; and a second stage separation step in which the second primary separation solution located in the second space continues to rotate at a predetermined high speed at a high speed in the second stage separation step a relationship, the second primary separation solution is separated into a first secondary separation solution and a second secondary separation solution, and the second secondary separation solution is separated into the third through a second transmission channel due to a centrifugal force factor. The space is slidably disposed between the first space and the second space, and the volume of the first space and the second space can be changed by sliding the pressing portion. The separation method according to claim 6, wherein the injection portion is a recoverable material, and the injection portion is automatically sutured after the solution is injected through the injection portion to prevent the solution from flowing backward. The separation method of claim 6, wherein a first leak-stop structure and a second leak-stop structure are disposed between the pressing portion and the first space and the second space. The separation method of claim 6, wherein the sensor is an optical sensor, and the centrifugal consumable is made of a transparent material, and the optical sensor can distinguish the light transmission and/or reflection degree. The location of the demarcation point. The separation method of claim 6, comprising the step of performing the separation method using the plurality of centrifugal consumables, wherein the third space of the centrifugal consumable is connected to the first space of the other centrifugal consumable, The note can be restarted in the second stage separation step. Enter the step to reach the loop.