TWI537039B - Pippette and nucleic acid purification apparatus - Google Patents

Description

Pipette and nucleic acid purification equipment

The invention relates to an aspiration tube and a nucleic acid purification device, in particular to an aspiration tube having a micro-adjustable gas density for stably sucking and injecting a liquid, and a nucleic acid purification device using the same.

When it comes to a large number of nucleic acid purification projects, it is necessary to use several suction and injection functions of the suction tube to complete many complicated steps to purify the nucleic acid. Usually, each biological sample sample is in charge of a reagent, a substance to be purified, an injection reagent, a substance to be purified, and the like in a purification process.

Since it is often necessary to perform a nucleic acid purification work for a plurality of samples from different sources at a time, it is a conventional practice to form a plurality of suction tube pieces into one suction module at a time by means of mold forming, and most of the structures are It is integrally formed, and it is not possible to individually adjust the airtightness of each suction pipe member or individually replace each suction pipe member. Therefore, after a period of use, there will always be a small number of one or two suction pipe fittings in the suction module to start the problem of insufficient air tightness. However, this few faulty suction pipe fittings cannot be replaced at any time. Or, if you make individual adjustments directly, you will need to repair the few defective fittings at this time, which will cause the entire group of suction modules to be replaced, and increase the warranty and cost. In addition, the above-mentioned conventional suction module also has a certain manufacturing difficulty because it needs to take into consideration the yield of each suction pipe fitting at the time of manufacture, and if any component (multi-finger suction pipe fitting) has flaws, it will also Increase the cost of heavy work.

The reason is that the inventor is aware of the above-mentioned issues, and he is dedicated to research and matching. With the application of the theory, a present invention which is reasonable in design and effective in improving the above-mentioned defects is finally proposed.

The main object of the present invention is to provide a nucleic acid purification device and a suction tube member thereof, so as to avoid the problem of inconvenience of the conventional suction tube member.

In order to achieve the above object, the present invention provides a suction tube member, comprising: a tubular body having a first opening and a second opening at two ends, wherein the first opening and the second opening communicate with each other in the tubular body Forming a cavity in the body, the inside of the tubular body further comprises an inner cavity wall and an outer pipe wall; at least one airtight ring disposed flatly on a gas-tight ring formed on the inner edge of the second opening And the airtight ring protrudes outwardly from the airtight ring receiving portion; a pressing nut is screwed to the second opening, and the pressing nut defines a corresponding and communicates with the second opening a hollow portion, the hollow portion is an engagement opening toward the tubular body, the engagement opening is surrounded by a pressing periphery facing an end surface of the tubular body, and the end surface of the second opening is formed with the tubular body Forming a gap therebetween, and the pressing peripheral edge is pressed against one side of the airtight ring of the airtight ring receiving portion, and the airtight ring pressed by the pressing peripheral edge has an inner edge facing the gas The center of the dense ring expands and is closely attached to the pusher a column body; and a pull member that is retractable and advanced to be slidably disposed in the cavity and the hollow portion of the body, wherein the pressing periphery is screwed to the second opening with the pressing nut The airtight ring is pressed against the airtight ring, so that the airtight ring is pressed to expand the inner edge of the airtight ring toward the center of the airtight ring for tightly attaching to a column of the pusher.

In order to achieve the above object, the present invention also provides a nucleic acid purification device, which The invention comprises a plurality of suction tube members as described above, the nucleic acid purification device comprising: a suction control module comprising a suction tube container receiving frame, comprising an upper plate body and a lower plate body, the plurality of suctions The tube member is disposed between the upper plate body and the lower plate body, and the upper plate body is coupled to the pulling member for driving the pulling member to move back and forth in the cavity of the body, and there is a lower portion of the lower plate body a retreating portion for retracting the suction sleeve; a lifting sub-module for controlling the upper and lower movements of the suction tube receiving frame; a reagent placing module comprising a translational sub-module And a test tube frame, the translation sub-module is configured to control a horizontal displacement of the reagent placement module under the suction control module, the test tube frame body is provided with a plurality of receiving portions for accommodating a plurality of test tubes And a magnetic component module, the magnetic component module can be disposed adjacent to the test tube or adjacent to the reagent placement module, and is disposed below the suction tube receiving frame for adsorbing a magnetic bead at intervals cell.

The gas density of the inside of the suction pipe member can be individually adjusted and optimized by the screw lock structure of the pressure nut and the airtight ring, so that the plurality of suction pipe members are used for In the above nucleic acid purification device, once one of the suction pipe fittings has a problem of poor internal gas density, it is not necessary to require a whole set of replacement warranty as in the prior art, and can be individually replaced, adjusted and repaired. The invention can effectively solve the problem of inconvenient warranty, and there is no need to worry about the problem that the suction pipe fitting manufacturing yield is too low.

For a better understanding of the features and technical aspects of the present invention, reference should be made to the accompanying drawings.

Please refer to FIG. 1A, FIG. 1B, FIG. 3A and FIG. 3B, the present invention An aspiration tube member A is provided, comprising: a tubular body 10, at least one airtight ring 20, a compression nut 30, and a pull member 40.

The first end opening 11 and the second opening 12 are respectively connected to each other in the longitudinal direction of the tubular body 10, and the first opening 11 and the second opening 12 communicate with each other to form a hollow cavity 13 inside the tubular body 10, which is tubular. The interior of the body 10 further includes a cavity wall 14 . Further, the cavity wall 14 is adjacent to the first opening 11 and extends toward the longitudinal center of the tubular body 10 and is contracted to define a cavity limiting wall 141. The tubular body 10 The exterior also includes an outer tube wall 15.

In addition, the outer tube wall 15 is adjacent to the second opening 12, and includes a threaded portion 151 and a side recessed portion 152 in the longitudinal direction of the tubular body 10 from the right to the left of FIG. 3A. The upper edge of the inner side of the side recessed portion 152 is a The nut restricting portion 1522; and the outer tube wall 15 is adjacent to the first opening 11 and also the end of the tubular body 10 near the first opening 11, and is a set portion 153, and the surrounding portion of the sleeve portion 153 is further A gas-tight convex portion 1531 is protruded, and the sleeve portion 153 can be used to sleeve a suction sleeve T, and the air-tight convex portion 1531 is used to strengthen the tightness between the suction sleeve T and the sleeve portion 153. Airtight effect.

The airtight ring 20 is generally a resilient plastic member that is disposed flat on a hermetic ring receiving portion 121 formed on the inner edge of the second opening 12.

The pressing nut 30 is screwed to the second opening 12, so that the pressing nut 30 also has a nut thread portion corresponding to the threaded portion 151 (not shown), and the pressing nut 30 also has a corresponding opening. The hollow portion 31 of the second opening 12 is also connected to the second opening 12 of the tubular body 10 as a joint opening 32. The joint opening 32 is surrounded by a pressing periphery 321 .

The pusher member 40 is preferably a rod or a column of pistons. In the embodiment, the pusher member 40 further includes a tail end side hole 41, a tail end notch 42 and a column body. 43. The trailing end side hole 41 is laterally passed through the column body 43 of the pulling member 40 at an end farther from the first opening 11, or the column body 43 at the end of the pulling member 40 at the outside of the tubular body 10. .

The tail end recess 42 and the trailing end side hole 41 are both located at the same end of the pusher member 40, and the trailing end side hole 41 is located in the longitudinal direction, and the trailing end recess 42 is located laterally on the side of the column body, which may contribute to Easy assembly between components.

The pulling member 40 can be pulled back and pushed forward and disposed in the cavity 13 and the hollow portion 31 of the body, so that when the pulling member 40 is inserted into the hollow portion 31 and the cavity 13 in the body, the pressing force is urgent. The periphery 321 is intended to be pressed against the airtight ring 20 as the compression nut 30 is screwed to the second opening 12, so that the airtight ring 20 is pressed against the airtight ring 20, thereby making the airtight ring 20 The inner edge 21 of the airtight ring is expanded toward the center of the airtight ring 20 for tightly attaching to the column body 43 of the pusher member 40 to achieve reinforcement of the airtight ring 20 against the column body 43 of the pusher member 40. The degree of tight fit contributes to the airtight effect. In the preferred embodiment, the airtight ring 20 is two, but not limited to this number.

In the above, when the present invention is to be used for aspirating reagents and solutions, the compression nut 30 can be slightly screwed onto one end of the tubular body 10 in advance, and the airtight ring 20 is also disposed in the second opening 12 of the tubular body 10. The suction sleeve T is also tightly sleeved on the sleeve portion 153. At this time, the tension member 40 can be inserted into the hollow portion 31 and the cavity 13 in the body, and pushed into the bottom to make the pusher 40 position. The hollow portion 31 or the other end of the cavity 13 in the body can penetrate deep into the inner cavity limiting wall 141, and since the airtight ring 20 and the airtight convex portion 1531 are respectively opposite to the column of the pushing member 40 Body 43 and the inner wall of the suction sleeve T The airtight effect is generated, so when the tip end of the suction sleeve T is inserted into a liquid level (not shown), the pull-out member 40 can be pulled back toward the second opening 12, and the outside of the suction tube member 10 can be pulled out. A pressure difference that can draw the liquid is generated, and the liquid to be aspirated is sucked into the suction sleeve T.

Preferably, the airtight ring 20 may protrude toward or be exposed to the second opening in the direction of the second opening 12, such that the pressing edge 321 may be downward due to the pressing nut 30. When the opening 12 is screwed tightly, a greater pressure is exerted on the airtight ring 20. Moreover, the pressing periphery 321 may also be a convex (not shown) surrounding the protruding opening 32, so that the pressing nut 30 may be caused by the convex portion occupying part of the volume and space. When the screw is locked, it can be pressed more tightly to the airtight ring 20, so that the degree of deformation of the airtight ring 20 is greater, and the inner edge 21 of the airtight ring is more expanded toward the center of the airtight ring 20, thereby being able to When the suction tube member A is provided with the pusher member 40, the airtight ring 20 is pressed more tightly against the column body 43 of the pusher member 40.

In addition, the outer side of the pressing nut 30 can also be laterally disposed with a limiting screw hole 33. The limiting screw hole 33 corresponds to the side recess portion 152, and the limiting screw hole 33 can be used to lock a screw member R. The screw member R can abut against the undercut bottom surface 1521 of the side recessed portion 152 to fix the screw lock of the pressurizing nut 30, and the nut retaining portion 1522 is configured to interfere with the screw member R. Or in the side recess 152 is closer to the second opening 12, so if the screw R is screwed into the limit screw hole 33, the press nut 30 will be replaced by the screw R and the nut limit portion. The interference of 1522 allows the compression nut 30 to be arbitrarily pulled out at any time.

Preferably, the hollow portion 31 of the compression nut 30 is surrounded by a nut inner side wall 34, and the inner side wall 34 of the nut is further provided with a guiding member 341 for guiding. The member 341 is preferably a resilient plastic member, but not limited thereto. When the pulling member 40 is pulled back and pushed in the cavity 13 and the hollow portion 31 of the body to perform a piston movement, Since the lead member 341 is in contact with the column body 43 of the pusher member 40, the skew which may be generated when the pusher member 40 performs the piston movement can be avoided, so as to assist the pusher member 40 to maintain a relatively linear piston motion, in other words, the guide The lead 341 helps to avoid structural errors caused by assembly between components and maintains the correctness of the trajectory of the component during movement, so it is not difficult to imagine that the guide 341 helps maintain structural correctness during assembly or movement. It is of course also possible to contribute to the tight fit of the airtight ring 20 to the cylinder 43 to promote airtightness. Therefore, in another angle, the guiding member 341 can guide the pulling member 40, thereby maintaining a tight fit between the pulling member 40 and the airtight ring 20.

Because the compression nut 30 achieves the tight airtight effect of the airtight ring 20 on the pusher 40 by means of a screw lock, the tight airtight effect is adjustable depending on the degree of tightening when the screw is locked. The gas-tight ring 20 is actually the key to the gas density of the present invention, but the air-tight ring 20 is easy to cause fatigue and aging problems of components due to long-term use. However, the gas is sterilized by the screw lock of the present invention. The degree of density becomes adjustable, and the airtightness can be achieved with a slight degree of screw lock before the airtight member such as the airtight ring 20 has not aged, so that the service life of the airtight ring 20 can be prolonged, and aging can be delayed. Even after the airtight ring 20 is aged, the user can perform a stronger screw locking and tightening action on the pressing nut 30 to promote the tight fitting of the airtight ring 20 to the cylinder 43 after aging, thereby achieving the reinforcing gas. The effect of the tightness can achieve the purpose of making the best use and saving cost.

Moreover, in order to obtain an optimal airtight effect, the above-mentioned push-pull member 40 of the present invention or its cylinder 43 can be coated between the airtight ring 20 or the guide member 341. It is greased, which can be a grease with high viscosity and low fluidity, such as: sealing oil, airtight oil, etc., so that the grease can form a strong oil film in the gap between the components, making the air less free. The ground is shuttled between the components to achieve the best airtight effect, and the grease can also produce a lubricating effect, which can effectively avoid the wear between the components and reduce the friction to produce a good operation effect.

Referring to the drawings, 2A, 2B and 3A, after adjusting the airtightness of the inner airtight ring 20 of the suction pipe member A to the column body 43 of the pusher member 40, the airtightness can be tested before use. Generally, the first opening 11 can be blocked in advance, and then the end of the pulling member 40 outside the suction pipe member A is externally pulled to test the degree of airtightness, and the required pulling force is usually airtight. Positively correlated, when the degree of pulling force is as large as a critical pulling force, which is enough to cause the gas density of the suction pipe member A to disintegrate, and the pulling member 40 can be smoothly extracted, the airtight ring 20 can be inferred from the critical pulling force at this time. The gas density caused by the entire set of suction tube members A; or a pressure gauge, also when the first opening 11 is blocked, a pushing force is applied to the pushing member 40 to test the suction in the sealed state. The maximum pressure inside the pipe member A, when the internal pressure is as large as a critical pressure, is sufficient to cause the gas density of the suction pipe member A to disintegrate, and the pusher member 40 can be smoothly pressed into the inner cavity of the suction pipe member A. At this time, the critical pressure can be inferred from the critical pressure to the entire set of suction pipe fittings A. The resulting gas density, in general the degree of airtightness usually positively correlated with the desired pressure.

The present invention further provides a nucleic acid purification device B comprising a plurality of aspiration tube members A as described above, the nucleic acid purification device B comprising: a suction control module 50, further comprising a suction tube holder 51 a lifting sub-module 52 for controlling the upper and lower movement of the suction pipe fitting holder 51, and a reagent setting The module 53 and a magnetic component module 54 are disposed.

The suction tube accommodating frame 51 includes an upper plate body 511 and a lower plate body 512, which are preferably, but not limited to, because the upper plate body 511 and the lower plate body 512 are respectively disposed above and below. The fixing hole (not labeled) is preferably, but not limited to, the plurality of suction tube members A are disposed between the upper plate body 511 and the lower plate body 512, and the upper plate body 511 is mechanically The rear end side hole 41 connected to the pusher member 40 is configured to drive the pusher member 40 to move back and forth in the cavity 13 in the body. Further, a lower portion of the lower plate body has a retreat portion 5121, and the retreat portion 5121 can be used downward. Pushing and utilizing the structural relationship (not shown) that longitudinally interferes with the wall of the suction sleeve T, and thereby withdrawing the suction sleeve T that has been installed in the sleeve portion 153.

The lifting sub-module 52 can be used to mechanically control the upper and lower movements of the suction pipe fitting holder 51 as a whole in the Y-axis direction, thereby adjusting the suction pipe member A or its suction casing T relative to the reagent placement module 53. the height of.

The reagent placement module 53 further includes a translational sub-module 531 and a test tube rack 532. The translational sub-module 531 can be used to control the horizontal displacement of the reagent placement module 53 itself in the X-axis and Z-axis directions below the suction tube holder 51, and the test tube holder 532 is further provided with a plurality of receiving portions 5321. The portion 5321 is for accommodating a plurality of tubes (not shown). However, the tubes referred to herein are not limited to the general tube, but also include centrifuge tubes of various capacities, as a preferred example is 1.5 mL. Microcentrifuge tube (eppendorf), but not limited to this.

The magnetic component module 54 can be disposed adjacent to the test tube or close to the reagent placement module 53. The magnetic component module 54 can be used to adsorb the wall of the tube. A magnetic bead cell to facilitate retention of the target cells to be purified during nucleic acid purification. And the above tube The wall is not limited and it may be the wall of any of the above test tubes.

In summary, the present invention can achieve the advantage of individually fine-tuning the air density of the suction tube member A during use, so that the entire module is not required to be replaced due to individual failures, and the warranty is simple and cost-effective. Because it can be adjusted individually, and because of its individual relationship, it can reduce heavy work in manufacturing and avoid unnecessary waste. However, the above description is only the preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. Therefore, the equivalent variations of the present invention or the contents of the drawings are equally applicable to the present invention. Within the scope of protection of rights, it is given to Chen Ming.

10‧‧‧Tube body

11‧‧‧ first opening

12‧‧‧ second opening

121‧‧‧Airtight ring housing

13‧‧‧ Body cavity

14‧‧‧ lumen wall

141‧‧‧ lumen wall

15‧‧‧External wall

151‧‧ Thread Department

152‧‧‧ Side depression

1521‧‧‧ underside

1522‧‧‧ Nut Limiting

153‧‧‧Setting Department

1531‧‧‧ airtight

20‧‧‧ airtight ring

21‧‧‧ airtight ring inner edge

30‧‧‧Compressed Nuts

31‧‧‧ Hollow

32‧‧‧ joint opening

321‧‧‧ pressing periphery

33‧‧‧Limited screw holes

34‧‧‧The inner side wall of the nut

341‧‧‧Guide

40‧‧‧ Pulling pieces

41‧‧‧End side hole

42‧‧‧End notch

43‧‧‧ column

50‧‧‧ suction control module

51‧‧‧About pipe fittings

511‧‧‧Upper body

512‧‧‧ lower plate

5121‧‧‧Return Department

52‧‧‧ Lifting submodule

53‧‧‧Reagent placement module

531‧‧‧ translation submodule

532‧‧‧Test tube rack

5321‧‧‧ 容 部

A‧‧‧ suction pipe fittings

B‧‧‧Nucleic acid purification equipment

R‧‧‧screws

T‧‧‧ suction casing

1A is an exploded perspective view of a suction pipe fitting of the present invention; FIG. 1B is an exploded perspective view of the suction pipe fitting of the present invention; FIG. 2A is a perspective assembled view of the suction pipe fitting of the present invention (FIG. 2A); 3A is a side cross-sectional view of the pipette member of the present invention; FIG. 3B is a side elevational cross-sectional view of the pipette member of the present invention; and FIG. A schematic view of the use of a nucleic acid purification device and its aspiration tube.

10‧‧‧Tube body

11‧‧‧ first opening

12‧‧‧ second opening

13‧‧‧ Body cavity

14‧‧‧ lumen wall

141‧‧‧ lumen wall

15‧‧‧External wall

151‧‧ Thread Department

152‧‧‧ Side depression

153‧‧‧Setting Department

1531‧‧‧ airtight

20‧‧‧ airtight ring

30‧‧‧Compressed Nuts

31‧‧‧ Hollow

32‧‧‧ joint opening

33‧‧‧Limited screw holes

34‧‧‧The inner side wall of the nut

341‧‧‧Guide

40‧‧‧ Pulling pieces

41‧‧‧End side hole

42‧‧‧End notch

43‧‧‧ column

R‧‧‧screws

T‧‧‧ suction casing

Claims (10)

A suction pipe member comprises: a tubular body, wherein the two ends are respectively a first opening and a second opening, wherein the first opening and the second opening communicate with each other to form a cavity in the body inside the tubular body, The inside of the tubular body further includes an inner cavity wall and an outer tube wall; at least one airtight ring is disposed on a hermetic ring receiving portion formed on the inner edge of the second opening, and the airtight ring is convexly convex The airtight ring receiving portion; a pressing nut that is screwed to the second opening, and the pressing nut defines a hollow portion corresponding to and communicating with the second opening, the hollow portion facing the tubular portion The body is a joint opening, and the joint opening is surrounded by a pressing periphery facing an end surface of the tubular body, and a gap is formed between the end surface of the tubular body forming the second opening, and the pressing periphery is formed Pressing against one side of the airtight ring that protrudes outwardly from the airtight ring receiving portion, and the inner ring of the airtight ring that is pressed by the pressing peripheral edge expands toward the center of the airtight ring, and closely fits a column attached to the pusher; and a pull member capable of pulling Back and forth for advancing and movably disposed in the body cavity and into the hollow portion. The suction tube member of claim 1, wherein the airtight ring portion protrudes from the second opening. The suction tube member of claim 1, wherein the pressing circumference is a protrusion protruding around the joint opening. The suction tube member according to the first aspect of the invention, wherein the outer tube wall comprises a threaded portion and a side recessed portion adjacent to the second opening, and a nut limiting portion is above the inner edge of the side recessed portion. The suction pipe fitting of the fourth aspect of the invention, wherein the outer side of the pressure nut is laterally disposed with a limiting screw hole, the limiting screw hole corresponding to the side recessed portion, wherein the limiting screw hole is used for A screw member is locked, whereby the screw member abuts against a concave bottom surface of the side recess portion. The suction tube member according to claim 5, wherein the nut limiting portion is for interference fitting with the screw member. The suction pipe member according to claim 1, wherein the hollow portion has a nut inner side wall, and the inner side wall of the nut is covered with a guiding member, and the guiding member guides the pulling member. A tight fit between the pusher and the airtight ring is maintained, and the guide member, the airtight ring and the column are coated with an airtight oil. The suction pipe fitting according to the first aspect of the invention, wherein the outer pipe wall is a set portion near the first opening, and the sleeve ring is provided with a gas-tight convex portion, and the airtight convex portion is used for The sleeve is sleeved tightly on a suction sleeve. The suction tube member according to claim 1, wherein the end portion of the pulling member located outside the tubular body is laterally provided with a tail end side hole. A nucleic acid purification device comprising a plurality of aspiration tube members according to any one of claims 1 to 9, the nucleic acid purification device comprising: a suction control module comprising a suction tube The rack includes an upper plate body and a lower plate body, wherein the plurality of suction pipe members are disposed between the upper plate body and the lower plate body, and the upper plate body is coupled to the pulling member for driving the pulling The pusher moves back and forth in the cavity of the body, and the lower part of the lower plate body further has a retreating portion for retreating the suction sleeve; and one for controlling the upper and lower movement of the suction tube member receiving frame Lifting times a reagent placement module, comprising a translational sub-module and a test tube frame, wherein the translational sub-module is configured to control a horizontal displacement of the reagent placement module under the suction control module, The test tube rack body is provided with a plurality of accommodating portions for accommodating a plurality of test tubes, and a magnetic component module, wherein the magnetic member module can be disposed adjacent to the test tube or can be disposed adjacent to the reagent placement module. Below the tube-receiving holder, a magnetic bead cell is adsorbed at intervals of one tube wall.