TWI535386B - Aquatic animal feed - Google Patents

Aquatic animal feed Download PDF

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TWI535386B
TWI535386B TW103110929A TW103110929A TWI535386B TW I535386 B TWI535386 B TW I535386B TW 103110929 A TW103110929 A TW 103110929A TW 103110929 A TW103110929 A TW 103110929A TW I535386 B TWI535386 B TW I535386B
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animal feed
aquatic animal
microalgae
carrier
probiotics
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TW103110929A
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TW201536186A (en
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Pin-Xing Zhou
Yu-Lun Liu
Cheng Su
xiao-fen Zhang
Pei-Yu Li
Jian-De Huang
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水生動物飼料 Aquatic animal feed

本發明係關於一種飼料,特別是指一種水生動物飼料。 The present invention relates to a feed, and more particularly to an aquatic animal feed.

益生菌(probiotics)是指能促進腸道微生物平衡的生物體或物質。一般而言,乳酸菌以及桿菌屬(Bacillus spp.)為最常見的益生菌。益生菌在水產養殖方面亦能產生相當好的效果,近來研究指出,餵食益生菌可以增進被餵食動物的免疫力及抵抗鏈球菌屬(Streptococcus sp.)、哈維氏弧菌(Vibrio harveyi)、黑美人弧菌(Vibrio nigripulchritudo)、副溶血弧菌(Vibrio parahaemolyticus)等常見病原菌的能力,例如石斑魚、草蝦等物種,並且提高被餵食動物的存活率。 Probiotics are organisms or substances that promote the balance of intestinal microbes. In general, lactic acid bacteria and Bacillus spp. are the most common probiotics. Probiotics can also produce good results in aquaculture. Recent studies have shown that feeding probiotics can improve the immunity of the animals being fed and resist Streptococcus sp. , Vibrio harveyi , The ability of common pathogens such as Vibrio nigripulchritudo and Vibrio parahaemolyticus , such as grouper, grass shrimp, etc., and improves the survival rate of the fed animals.

一般在水生動物的飼料中添加益生菌有兩種方法,一是將乾燥後的益生菌粉末直接混入飼料當中,二則是在飼料加工的過程中混入原料當中一同加工。然而,直接混入益生菌粉末所製成的飼料,投放到水中之後,益生菌粉末便容易漂散在各處,因此造成餵食對象攝入益生菌的機率非常低。共同加工所製成的飼料雖然能夠將益生菌包埋於其中,而提高被餵食對象攝入益生菌的攝食率,但是在製造的過程中容易因為加工時的高溫而破壞益生菌的活性。此外,一般飼料並非水生動物在原生環境中的食物,食用此飼料易有營養不均之問題。 Generally, there are two methods for adding probiotics to the feed of aquatic animals. One is to directly mix the dried probiotic powder into the feed, and the other is to process the raw materials together in the feed processing process. However, the feed made from the probiotic powder directly mixed into the water, the probiotic powder is easily dispersed everywhere, so the probability of feeding the probiotics to the feeding object is very low. Although the feed produced by the co-processing can embed the probiotics therein, the feeding rate of the probiotics ingested by the feeding object is improved, but the activity of the probiotics is easily destroyed by the high temperature during processing in the manufacturing process. In addition, the general feed is not a food of aquatic animals in the original environment, and eating the feed is prone to nutritional malnutrition.

有鑑於上述問題,習用的水生動物飼料顯然有加以改良之需要。 In view of the above problems, the conventional aquatic animal feed obviously needs to be improved.

本發明提供一種水生動物飼料,能有效增進被餵食對象對於益生菌的攝食率,並且保留益生菌的活性,同時避免營養不均的問題。 The invention provides an aquatic animal feed, which can effectively improve the feeding rate of the probiotics to the fed object, and retain the activity of the probiotics while avoiding the problem of uneven nutrition.

為達成上述目的,本發明提供一種水生動物飼料,包含有至少一載體、一薄膜層以及複數益生菌,其中各該載體係微藻之乾燥遺體,該薄膜層係包覆於該載體,該等益生菌係位於該薄膜層中。 In order to achieve the above object, the present invention provides an aquatic animal feed comprising at least one carrier, a film layer and a plurality of probiotics, wherein each of the carriers is a dried body of microalgae, the film layer is coated on the carrier, and the like Probiotic strains are located in the film layer.

藉此,透過可供水生動物食用的微藻作為載體,並使得益生菌在室溫條件下聚集在載體表面,形成包覆整個載體的薄膜層,讓益生菌能夠大量附著在載體表面,並且保有其原有的活性,使得被餵食對象能夠在吃進載體的同時,攝取到大量的益生菌,又不會有營養不均的問題。 Thereby, the microalgae which can be eaten by aquatic animals is used as a carrier, and the probiotics are aggregated on the surface of the carrier at room temperature to form a film layer covering the entire carrier, so that the probiotic bacteria can be attached to the surface of the carrier in a large amount, and retained. Its original activity enables the fed object to ingest a large amount of probiotics while eating the carrier without the problem of uneven nutrition.

有關本發明之詳細特點,將於後續之詳細說明中予以描述。然而,在本發明領域中具有通常知識者應能瞭解,該等詳細說明以及實施本發明所列舉之特定實施例,僅用於說明本發明,並非用以限制本發明之專利申請範圍。 Detailed features of the invention will be described in the detailed description which follows. However, it should be understood by those of ordinary skill in the art that the present invention is not limited by the scope of the invention.

10‧‧‧水生動物飼料 10‧‧‧Aquatic animal feed

20‧‧‧載體 20‧‧‧ Carrier

30‧‧‧薄膜層 30‧‧‧film layer

40‧‧‧益生菌 40‧‧‧Probiotics

第1圖係本發明一較佳實施例之示意圖,主要顯示單一載體被薄膜層包覆的情況。 BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a schematic view of a preferred embodiment of the present invention, mainly showing the case where a single carrier is covered by a film layer.

第2圖係本發明一較佳實施例之示意圖,主要顯示載體表面附著有益生菌的情況。 Fig. 2 is a schematic view showing a preferred embodiment of the present invention, mainly showing the case where a probiotic is attached to the surface of the carrier.

第3圖係本發明另一較佳實施例之示意圖,主要顯示多個載體被薄膜層包覆的情況。 Figure 3 is a schematic view of another preferred embodiment of the present invention, mainly showing the case where a plurality of carriers are covered by a film layer.

以下將藉由所列舉之實施例,配合隨附之圖式,詳細說明本發明之技術內容及特徵。 The technical contents and features of the present invention will be described in detail below with reference to the accompanying drawings.

如第1圖至第2圖所示,本發明一較佳實施例所提供之水生動物飼料10包含有一載體20、一薄膜層30以及多數個益生菌40。 As shown in Figures 1 to 2, the aquatic animal feed 10 according to a preferred embodiment of the present invention comprises a carrier 20, a film layer 30 and a plurality of probiotics 40.

載體20係扁藻(Tetraselmis sp.)、小球藻(Chlorella spp.)、擬球藻(Nannochloropsis sp.)、角刺藻(Chaetoceros sp.)、骨條藻(Skeletonema sp.)、等鞭金藻(Isochrysis sp.)、裂壺藻(Schizochytrium sp.)、螺旋藻(Spirulina sp.)或席藻(Phormidium sp.)等不同種類的微藻(microalgae)其中一種,這些種類的微藻對於水生動物具有較佳的適口性。其中,作為載體20的微藻係已經死亡並經過乾燥處理,為微藻的乾燥遺體,且微藻的最大外徑係介於大於或等於3微米至小於或等於500微米之間。 The carrier 20 is a strain of Tetraselmis sp. , Chlorella spp. , Nannochloropsis sp. , Chaetoceros sp. , Skeletonema sp. , isotonic gold One of different kinds of microalgae such as Isochrysis sp. , Schizochytrium sp. , Spirulina sp. or Phormidium sp. , these species of microalgae are aquatic Animals have better palatability. Wherein, the microalgae system as the carrier 20 has died and is dried to be a dried remains of the microalgae, and the maximum outer diameter of the microalgae is between 3 micrometers or more and 500 micrometers or less.

薄膜層30係一層由益生菌40分泌的胞外基質(extracellular polymeric substance;EPS)所構成的生物膜(biofilm),其成形於各個載體20的表面,而包覆整個載體20,如第1圖所示。其中,薄膜層30係將載體20與益生菌40放置在室溫下共同培養24小時,即可在各個載體20的表面生成一層薄膜層30。 The film layer 30 is a biofilm composed of an extracellular polymeric substance (EPS) secreted by the probiotic 40, which is formed on the surface of each carrier 20, and covers the entire carrier 20, as shown in FIG. Shown. Here, the film layer 30 is formed by placing the carrier 20 and the probiotic 40 together at room temperature for 24 hours to form a film layer 30 on the surface of each carrier 20.

複數個益生菌40則分佈在薄膜層30中,本實施例所使用的這些益生菌40係比菲德氏菌(Bifidobacterium bifidum)、糞腸球菌(Enterococcus faecalis)、屎腸球菌(Enterococcus faecium)、乳酸腸球菌(Enterococcus lactis)、嗜酸乳桿菌(Lactobacillus acidophilus)、乾酪乳酸桿菌(Lactobacillus casei)、乳酸球菌(Lactobacillus lactis)、胚芽乳酸桿菌(Lactobacillus plantarum)、百得酸小球菌(Pediococcus acidilactici)、乳酸四鏈球菌(Pediococcus pentosaceus)、枯草桿菌(Bacillus subtilis)、異質發酵菌(Phodopseudomonas pulustris)或地衣芽孢桿菌(Bacillus licheniformis)等菌種中的一種或多種的混合。 A plurality of probiotics 40 are distributed in the film layer 30, and the probiotics 40 used in the present embodiment are Bifidobacterium bifidum , Enterococcus faecalis , Enterococcus faecium , Enterococcus lactis , Lactobacillus acidophilus , Lactobacillus casei , Lactobacillus lactis , Lactobacillus plantarum , Pediococcus acidilactici , A mixture of one or more of a strain such as Pediococcus pentosaceus , Bacillus subtilis , Phodopseudomonas pulustris , or Bacillus licheniformis .

第3圖係本發明另一實施例,主要說明本發明所提供的水生動物飼料10並不僅限於由單一載體20所組成,亦得由多個載體20組成,各個載體20分別被薄膜層30所包 覆,且薄膜層30內分佈有多個益生菌40。其中,各個載體20可為相同種類的微藻,或者各個載體20分別為不同種類的微藻,而組成含有單一微藻或者是含有一種以上不同種類微藻的水生動物飼料10。 3 is another embodiment of the present invention, mainly illustrating that the aquatic animal feed 10 provided by the present invention is not limited to being composed of a single carrier 20, but also consists of a plurality of carriers 20, each of which is respectively covered by a film layer 30. package Covered, and a plurality of probiotics 40 are distributed in the film layer 30. Wherein, each of the carriers 20 may be the same kind of microalgae, or each of the carriers 20 is a different kind of microalgae, and the composition contains a single microalgae or an aquatic animal feed 10 containing one or more different kinds of microalgae.

由於微藻具有豐富的蛋白質、不飽和脂肪酸、纖維質、維生素以及礦物質,原本即是許多淡水與海水生物的主要食物來源,尤其是幼魚和無脊椎動物。因此,以微藻作為益生菌40的載體20,係應用微藻可供水生動物食用,具有較佳的適口性、營養成分以及懸浮性,再透過益生菌40於載體20表面聚集形成生物膜,使得益生菌40能夠藉由薄膜層30大量地附著在載體20的表面,讓被餵食的水生動物,例如南美白對蝦(Litopenaeus vannamei)、草蝦(Penaeus monodon)、中國對蝦(Penaeus chinensis)、斑節蝦(Penaeus japonicus)等,吃進載體20的同時也能夠吃進大量附著在載體20表面的益生菌40,而達到增加被餵食對象對於益生菌40的攝食率,並且由於薄膜層30的生成係在室溫的條件下,因此在飼料的製造過程中,不會破壞益生菌40原有的活性,而仍能有效保留飼料中益生菌40的活性,且可避免長期食用一般飼料所引發的營養不均問題。 Because microalgae are rich in protein, unsaturated fatty acids, cellulosics, vitamins and minerals, they are the main source of food for many freshwater and marine organisms, especially juveniles and invertebrates. Therefore, the microalgae is used as the carrier 20 of the probiotic 40, and the microalgae is used for aquatic animals to eat, has better palatability, nutrient composition and suspension property, and then accumulates on the surface of the carrier 20 to form a biofilm through the probiotic 40. The probiotic 40 is allowed to adhere to the surface of the carrier 20 in a large amount by the film layer 30, and the aquatic animals to be fed, such as Litopenaeus vannamei , Penaeus monodon , Penaeus chinensis , and plaque Shrimp ( Penaeus japonicus ), etc., can also eat a large amount of probiotics 40 attached to the surface of the carrier 20 while eating the carrier 20, thereby increasing the feeding rate of the probiotics 40 by the feeding object, and due to the formation of the film layer 30. It is at room temperature, so it does not destroy the original activity of probiotic 40 during the manufacturing process of the feed, but still effectively retains the activity of probiotic 40 in the feed, and avoids the long-term consumption of general feed. Unequal nutrition problems.

在此必須說明者為,以上配合圖式所為之詳細描述,僅係為了說明本發明之技術內容及特徵而提供之若干實施方式,凡在本發明領域中具有一般通常知識之人,在瞭解本發明之技術內容及特徵之後,於不違背本發明之精神下,所為之種種簡單之修飾、替換或構件之減省,皆應屬於以下所揭示之申請專利範圍之內。 It is to be understood that the foregoing detailed description of the drawings is merely illustrative of the embodiments of the invention </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt;

10‧‧‧水生動物飼料 10‧‧‧Aquatic animal feed

20‧‧‧載體 20‧‧‧ Carrier

30‧‧‧薄膜層 30‧‧‧film layer

40‧‧‧益生菌 40‧‧‧Probiotics

Claims (4)

一種水生動物飼料,包含有:至少一載體,各該載體係微藻之乾燥遺體;一薄膜層,包覆於各該載體;以及複數益生菌,位於該薄膜層中。 An aquatic animal feed comprising: at least one carrier, each of which is a dried body of microalgae; a film layer coated on each of the carriers; and a plurality of probiotics located in the film layer. 如請求項1所述之水生動物飼料,其中該微藻係扁藻(Tetraselmis sp.)、小球藻(Chlorella spp.)、擬球藻(Nannochloropsis sp.)、角刺藻(Chaetoceros sp.)、骨條藻(Skeletonema sp.)、等鞭金藻(Isochrysis sp.)、裂壺藻(Schizochytrium sp.)、螺旋藻(Spirulina sp.)或席藻(Phormidium sp.)。 The aquatic animal feed according to claim 1, wherein the microalgae are Tetraselmis sp. , Chlorella spp. , Nannochloropsis sp. , Chaetoceros sp. , Skeletonema sp. , Isochrysis sp. , Schizochytrium sp. , Spirulina sp. or Phormidium sp . 如請求項2所述之水生動物飼料,其中該微藻的外徑係介於大於或等於3微米至小於或等於500微米之間。 The aquatic animal feed according to claim 2, wherein the microalgae has an outer diameter of from greater than or equal to 3 micrometers to less than or equal to 500 micrometers. 如請求項1所述之水生動物飼料,其中各該益生菌係比菲德氏菌(Bifidobacterium bifidum)、糞腸球菌(Enterococcus faecalis)、屎腸球菌(Enterococcus faecium)、乳酸腸球菌(Enterococcus lactis)、嗜酸乳桿菌(Lactobacillus acidophilus)、乾酪乳酸桿菌(Lactobacillus casei)、乳酸球菌(Lactobacillus lactis)、胚芽乳酸桿菌(Lactobacillus plantarum)、百得酸小球菌(Pediococcus acidilactici)、乳酸四鏈球菌(Pediococcus pentosaceus)、枯草桿菌(Bacillus subtilis)、異質發酵菌(Phodopseudomonas pulustris)或地衣芽孢桿菌(Bacillus licheniformis)。 The aquatic animal feed according to claim 1, wherein each of the probiotic strains Bifidobacterium bifidum , Enterococcus faecalis , Enterococcus faecium , Enterococcus lactis , Lactobacillus acidophilus , Lactobacillus casei , Lactobacillus lactis , Lactobacillus plantarum , Pediococcus acidilactici , Pediococcus pentosaceus ), Bacillus subtilis , Phodopseudomonas pulustris or Bacillus licheniformis .
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