Claims (19)
一種幾丁聚醣微膠囊之製造方法，至少包含：提供胜肽溶液，其中該胜肽溶液包括分子量小於1200kDa之胜肽，且該胜肽為魚類膠原蛋白水解物；將幾丁聚醣溶液與該胜肽溶液混合，以形成一第一混合溶液，其中該幾丁聚醣溶液包括0.01重量/體積百分比(%wt/v)至5重量/體積百分比之幾丁聚醣，且該第一混合溶液之酸鹼值(pH)係小於5.5；將三聚磷酸鈉溶液加入該第一混合溶液中，以形成一第二混合溶液，其中該三聚磷酸鈉與該幾丁聚醣之重量比係介於10至100之間，且該第二混合溶液之酸鹼值係大於5.5；以及進行一乳化反應，對該第二混合溶液進行一超音波處理步驟，使該幾丁聚醣包覆該胜肽，以形成含有該幾丁聚醣微膠囊之一懸浮液，其中該幾丁聚醣微膠囊具有一多孔狀結構但不包含海藻酸及/或氯化鈣。
A method for producing a chitosan microcapsule, comprising: providing a peptide solution, wherein the peptide solution comprises a peptide having a molecular weight of less than 1200 kDa, and the peptide is a fish collagen hydrolyzate; the chitosan solution is The peptide solution is mixed to form a first mixed solution, wherein the chitosan solution comprises 0.01 weight/volume percentage (% wt/v) to 5 weight/volume percent chitosan, and the first mixture The pH value of the solution is less than 5.5; the sodium tripolyphosphate solution is added to the first mixed solution to form a second mixed solution, wherein the weight ratio of the sodium tripolyphosphate to the chitosan is Between 10 and 100, and the pH value of the second mixed solution is greater than 5.5; and performing an emulsification reaction, performing an ultrasonic treatment step on the second mixed solution to coat the chitosan The peptide is peptided to form a suspension containing the chitosan microcapsules having a porous structure but no alginic acid and/or calcium chloride.
如申請專利範圍第1項所述之幾丁聚醣微膠囊之製造方法，其中該胜肽包含98重量百分比分子量小於1200kDa的該胜肽。
The method for producing a chitosan microcapsule according to claim 1, wherein the peptide comprises 98% by weight of the peptide having a molecular weight of less than 1200 kDa.
如申請專利範圍第1項所述之幾丁聚醣微膠囊之製造方法，其中該幾丁聚醣之去乙醯化程度係大於75%。
The method for producing a chitosan microcapsule according to claim 1, wherein the degree of deacetylation of the chitosan is greater than 75%.
如申請專利範圍第1項所述之幾丁聚醣微膠囊之製
造方法，其中該幾丁聚醣溶液之一溶劑係選自於由醋酸、鹽酸、甘醇酸以及丙烯酸所組成之一族群。
The system of chitosan microcapsules as described in claim 1
The method, wherein the solvent of one of the chitosan solutions is selected from the group consisting of acetic acid, hydrochloric acid, glycolic acid and acrylic acid.
如申請專利範圍第1項所述之幾丁聚醣微膠囊之製造方法，其中該三聚磷酸鈉溶液包括1重量/體積百分比至6重量/體積百分比之三聚磷酸鈉。
The method for producing a chitosan microcapsule according to claim 1, wherein the sodium tripolyphosphate solution comprises from 1% by weight to 6% by weight of sodium tripolyphosphate.
如申請專利範圍第1項所述之幾丁聚醣微膠囊之製造方法，其中該超音波處理步驟係利用40至100瓦之功率進行30分鐘。
The method for producing a chitosan microcapsule according to claim 1, wherein the ultrasonic treatment step is performed for 30 minutes using a power of 40 to 100 watts.
如申請專利範圍第1項所述之幾丁聚醣微膠囊之製造方法，在該超音波處理步驟之後，更至少包含：對該幾丁聚醣微膠囊懸浮液進行一離心步驟，以獲得該幾丁聚醣微膠囊。
The method for producing a chitosan microcapsule according to claim 1, after the ultrasonic treatment step, at least comprising: performing a centrifugation step on the chitosan microcapsule suspension to obtain the Chitosan microcapsules.
如申請專利範圍第1項所述之幾丁聚醣微膠囊之製造方法，在將進行該乳化反應之前，更至少包含：將一油相溶液與該三聚磷酸鈉溶液依序加入該第一混合溶液中，以形成一第三混合溶液。
The method for producing a chitosan microcapsule according to claim 1, wherein before the emulsification reaction, the method further comprises: sequentially adding an oil phase solution and the sodium tripolyphosphate solution to the first The solution is mixed to form a third mixed solution.
如申請專利範圍第8項所述之幾丁聚醣微膠囊之製造方法，其中該油相溶液之酸鹼值係等於或小於5.5。
The method for producing a chitosan microcapsule according to claim 8, wherein the oil phase solution has a pH of equal to or less than 5.5.
如申請專利範圍第8項所述之幾丁聚醣微膠囊之製造方法，其中該油相溶液包含一界面活性劑與一油脂類化合物。
The method for producing a chitosan microcapsule according to claim 8, wherein the oil phase solution comprises a surfactant and a lipid compound.
如申請專利範圍第10項所述之幾丁聚醣微膠囊之製造方法，其中該界面活性劑為非離子型界面活性劑。
The method for producing a chitosan microcapsule according to claim 10, wherein the surfactant is a nonionic surfactant.
如申請專利範圍第10項所述之幾丁聚醣微膠囊之製造方法，其中該油脂類化合物係選自橄欖油、綿羊油以及上述之任意組合所組成之一族群。
The method for producing a chitosan microcapsule according to claim 10, wherein the oil-and-fat compound is selected from the group consisting of olive oil, lanolin, and any combination thereof.
如申請專利範圍第8項所述之幾丁聚醣微膠囊之製造方法，其中該乳化反應更至少包含：對該第三混合溶液進行超音波處理步驟，以形成該幾丁聚醣微膠囊懸浮液。
The method for producing a chitosan microcapsule according to claim 8, wherein the emulsification reaction further comprises: performing an ultrasonic treatment step on the third mixed solution to form the chitosan microcapsule suspension liquid.
如申請專利範圍第13項所述之幾丁聚醣微膠囊之製造方法，其中該超音波處理步驟係利用40至100瓦之功率進行30分鐘。
The method for producing a chitosan microcapsule according to claim 13, wherein the ultrasonic treatment step is performed for 30 minutes using a power of 40 to 100 watts.
如申請專利範圍第13項所述之幾丁聚醣微膠囊之製造方法，在該超音波處理步驟之後，更至少包含：對該幾丁聚醣微膠囊懸浮液進行一離心步驟，以獲得該幾丁聚醣微膠囊。
The method for producing a chitosan microcapsule according to claim 13, after the ultrasonic treatment step, at least comprising: performing a centrifugation step on the chitosan microcapsule suspension to obtain the Chitosan microcapsules.
一種幾丁聚醣微膠囊，其係根據申請專利範圍第1項至第15項任一項之方法而製得，其中該幾丁聚醣微膠囊不包含海藻酸及/或氯化鈣。
A chitosan microcapsule obtained by the method according to any one of claims 1 to 15, wherein the chitosan microcapsule does not contain alginic acid and/or calcium chloride.
如申請專利範圍第16項所述之幾丁聚醣微膠囊，其中該幾丁聚醣微膠囊之一平均粒徑為1奈米(nm)至1微米(μm)之間。
The chitosan microcapsule according to claim 16, wherein one of the chitosan microcapsules has an average particle diameter of between 1 nanometer (nm) and 1 micrometer (μm).
如申請專利範圍第16項所述之幾丁聚醣微膠囊，其中該幾丁聚醣微膠囊兼具抑制金黃色葡萄球菌生長以及皮膚抗皺的效用。
The chitosan microcapsule according to claim 16, wherein the chitosan microcapsule has the same effect of inhibiting growth of Staphylococcus aureus and skin wrinkle resistance.
一種化妝品組合物，其包含根據申請專利範圍第16項至第18項任一項所述之幾丁聚醣微膠囊。
A cosmetic composition comprising the chitosan microcapsule according to any one of claims 16 to 18.