TWI425126B - Chitosan fiber matrix and preparing method and use thereof - Google Patents

Description

Chitosan fiber substrate, preparation method and use thereof

The invention relates to a method for preparing a fibrous substrate, and in particular to a method for preparing a chitosan fiber substrate.

There are usually two methods for preparing chitosan fibers, one is wet spinning and the other is electric spinning. However, the two spinning methods do not require the use of a high concentration of strong acid and alkali, that is, the use of an organic solvent to perform the spinning work, making the environmental protection problem more difficult to handle. The chitosan fiber obtained by the wet spinning method has a micron size, generally requires a non-woven process to form a fibrous substrate, and the neutral alkali or organic solvent is cleaned in the process, which also makes the process of the fiber substrate more complicated. . Although electrospinning can obtain nanometer-sized fibers, the use of more toxic organic solvents or high concentrations of organic acids in the process, as well as expensive process equipment and quantitative maturity, requires further consideration of nano-butanose fiber requirements. The necessity.

The chitosan of a single fiber type has a better mechanical property than other types, but its swelling property is also limited. While sponges, gels, and the like have better swelling properties, chemical modifiers or chemical crosslinking agents are required to maintain their structural stability. The above chemical crosslinking agents and chemical modifiers are not often complicated and difficult to control, and are often toxic. In addition, a single fiber or sponge structure has limited physical and chemical properties and functionality.

Accordingly, one aspect of the present invention is to provide a method of preparing a chitosan fiber substrate. The preparation method comprises the following steps.

According to one embodiment, the spinning solution and the molding liquid are separately prepared. The spinning solution comprises an aqueous solution of lactic acid of chitosan, wherein the concentration of chitosan and lactic acid is about 0.1-2.5 w/v%, respectively. The above molding liquid contains an aqueous solution of a weak base, and the concentration of the weak base is 0.1 to 2.5 w/v%. The above weak base may be, for example, tris(hydroxymethyl)aminomethane (referred to as Tris base). Next, the spinning is dropped into the molding liquid, and rapid stirring is carried out to solidify the spinning solution into fibers, and the fibers are uniformly distributed in the molding liquid to form a chitosan fiber solution. Then, the chitosan fiber solution is subjected to a step of molding drying to form a fibrous substrate.

According to another embodiment, the rapid stirring step requires the Reynolds number of the molding liquid to be 1.47 × 10 ^{4 -} 1.176 × 10 ⁵ to allow the chitosan to form fibers having a diameter of 10 μm or less.

Therefore, according to the above embodiment, since the low concentration of weak acid and weak base is used, the environmental problem of the conventional wet spinning/electrospinning can be solved. Further, since the molding liquid containing the chitosan fiber is directly subjected to the molding and drying step, the process of the fiber base material can be simplified.

The Summary of the Invention is intended to provide a simplified summary of the present disclosure in order to provide a basic understanding of the disclosure. This Summary is not an extensive overview of the disclosure, and is not intended to be an The basic spirit and other objects of the present invention, as well as the technical means and implementations of the present invention, will be readily apparent to those skilled in the art of the invention.

According to the above, a method for preparing a chitosan fiber substrate is provided. The method for preparing the chitosan fiber substrate uses a weak acid and a weak base to obtain chitosan fibers having a diameter of about 1-10 μm. In the following description, an exemplary manufacturing method of the chitosan fiber substrate described above will be described. In order to facilitate an understanding of the described embodiments, a number of technical details are provided below. Of course, not all embodiments require these technical details. At the same time, some well-known structures or elements are only shown in the drawings in a schematic manner to appropriately simplify the contents of the drawings.

Method for preparing chitosan fiber substrate

Fig. 1 is a flow chart showing the preparation of a chitosan fiber substrate according to an embodiment of the present invention. In step 110, a spinning solution is prepared, and the spinning solution contains an aqueous solution of lactic acid of chitosan. According to one embodiment, the pH of the lactic acid aqueous solution of the chitosan is less than the isoelectric point of chitosan (pKa is 6.0-6.5) to completely dissolve the chitosan. In addition, the concentration of chitosan needs to be less than 2.5 w/v% to avoid the formation of a gel of chitosan, and the subsequent step of forming fibers is not possible. Therefore, the concentration of the above chitosan and the concentration of lactic acid are respectively about 0.1 to 2.5 w/v%.

According to another embodiment, the above spinning solution may further comprise at least one functional ingredient to increase the efficacy of the resulting fibrous substrate. The amount of the above functional component added may be, for example, 25 wt% or less of the weight of chitosan, so as not to affect the stability and moisture absorption/moisture retention of the chitosan fiber substrate.

For example, if it is desired to increase the structural stability of the finally obtained fibrous substrate, a polymer having an acid functional group may be added to form a polyelectrolyte complex with chitosan to increase the stability of the fibrous substrate structure. . The above polymer having an acid functional group may be, for example, carboxymethyl cellulose (CMC), hyaluronic acid or alginate. For example, if it is desired to increase the toughness of the finally obtained fibrous substrate, polyethylene glycol may be added. If it is desired to increase the physiological efficacy of the finally obtained fibrous substrate, for example, collagen, gelatin, coagulation factor or growth factor may be added.

In step 120, a molding fluid is prepared which comprises an aqueous solution of a weak base having a pH of about 9-12. The shape obtained by solidifying chitosan in the molding liquid is mainly composed of fibers having a diameter of 10 μm or less, and the concentration of the weak base may be 3.0 w/v% or less, for example, 0.1 to 2.5 w/v%. The weak base may be, for example, tris(hydroxymethyl)aminomethane or alanine, ammonia, methylamine, pyridine, aqueous ammonia, aluminum hydroxide, copper hydroxide, ferrous hydroxide, iron hydroxide, zinc hydroxide, or the like. .

In step 130, the spinning solution is dropped into a molding liquid and simultaneously stirred rapidly, and the spinning solution is solidified into chitosan fibers to form a chitosan fiber solution. In this step, in order to form a chitosan fiber having a diameter of 10 μm or less, it is necessary to control the droplet size of the spinning solution and the shear stress generated by the molding liquid when the molding liquid is stirred to control. .

The droplet size and flow rate of the above-mentioned spinning solution can be supplied to the molding liquid by allowing the spinning solution to pass through the spun size and the gear pump to provide a stable droplet size and flow rate. The above-mentioned spun orifice may have a pore diameter of 1-100 μm, for example, about 10 μm, and the gear pump may have a flow rate of 5 to 100 ml/min, for example, about 20 ml/min.

The shear stress generated by the molding liquid during the mixing is controlled by the design of the stirring blade and the stirring tank in the stirring device, so that the droplets of the spinning solution are dispersed into the micron level in the molding liquid due to the high shear stress. The droplets are dragged into a fibrous shape. The shear stress generated by the molding liquid during the stirring process is related to the Reynolds number (N _Re ; Reynolds number) in the following formula (1):

Reynolds number = inertial force / viscous force = ρuD / μ (1)

Where ρ in the Reynolds number definition is the density of the fluid (in kg/m ³ ), the velocity u of the fluid is often represented by the wing end speed nD of the agitating wing, the rotational speed of the n agitating wing (in rps), and D is the wing of the agitating wing The diameter (in m), μ is the viscosity of the fluid (in kg/ms), and the Reynolds number (symbol N _Re ) is as shown in the following equation (2):

N _Re =ρnD ² /μ (2)

It can be seen from the above formula that the Reynolds number of the molding liquid in the stirring tank varies with the geometric design of the stirring blade. Here, it is necessary to cause the molding liquid to generate high shear stress to disperse the droplets into micron grades and to be dragged into a fiber type, but too high shear stress will cause the fibers dispersed by the droplets to be broken after molding, thus resulting in The structure is scattered. Therefore, in the case where the molding liquid is an aqueous solution, the density (1000 kg/m ³ ) and viscosity (1.02 × 10 ⁶ kg/ms) of the fluid are estimated by water to calculate a preferred Reynolds number range. In addition, according to the formula (2), the range of the rotational speed of the stirring wing should be estimated according to the diameter of the stirring wing. For example, if the diameter of the agitating wing is 0.03 m, the speed of the agitating blade may be 16.6-13.33 rps, that is, about 1,000-8,000 rpm.

In addition, considering the power consumption of the stirring wing, it is desirable to achieve energy saving, and it is conceivable to use a high shearing agitating wing. Common high shear agitating wings such as sawtooth-edge modified turbines, modified turbines, strait balds, screw impellers or auger impellers, double ribbon impellers (double spiral ribbon impeller).

The end time of the execution time of step 130 is judged by the pH value of the chitosan fiber solution, that is, when the spinning solution is added to the molding liquid, the pH value of the last chitosan fiber solution is about pH. At about 7 or so, that is, at pH 6.5-7.5, step 130 is stopped. At this time, if the chitosan spinning solution is further added, the chitosan fiber is formed because the pH value of the chitosan fiber solution is too low, less than its isoelectric point, and is not enough for the spinning solution to solidify.

Next, step 140 is performed to allow the chitosan fiber solution to undergo various different molding drying steps to form a variety of different fibrous substrates. For example, when the fiber substrate is in the form of a sponge, a thin film, a hydrocolloid or a hydrogel, the chitosan fiber solution can be first injected into a suitable mold. The chitosan fiber solution in the mold is then dried in different ways. The drying method of the sponge is freeze-drying, the drying method of the film is vacuum heating and drying, the way of colloid is hot air drying, and the way of water gel is drying at room temperature.

As can be seen from step 140, the chitosan fibers have been distributed in the chitosan fiber solution during molding drying. Therefore, after drying and forming, these chitosan fibers have been intertwined with each other, so that no non-woven process or chemical crosslinking agent is needed to stabilize the structure of the fiber substrate, and at the same time, it has a liquid absorbing/moisturizing function. Clearance space.

Experimental examples of chitosan fiber substrates will be presented below to investigate the effects of chitosan concentration, weak base concentration, stirring rate and concentration of various functional components on chitosan fiber type.

Experimental Example 1: Effect of Chitosan Concentration in Spinning Solution on Chitosan Fiber Type

The chitosan has a molecular weight of about 930,000 and a deacetylation degree of greater than 75%. The concentration of chitosan and lactic acid in the spinning solution were respectively 0.1-2.5 w/v%, and the molding liquid was a 1 w/v% aqueous solution of tris(hydroxymethyl)aminomethane. The agitating wing has a diameter of 3 cm and a stirring rate of 4,000 rpm to form a chitosan fiber solution. Then, a solution containing 2 w/v% of chitosan fiber was poured into a mold and freeze-dried to obtain a sponge-type fibrous substrate.

From the results, it is understood that when the concentration of chitosan is 0.1-2.5 w/v%, the larger the concentration, the thicker the chitosan fiber is, about 1-10 μm. Moreover, in the product, a small amount of a sheet-like structure is doped, but it also increases as the concentration of chitosan increases. These sheet-like structures can join the fiber structures to increase the strength and stability of the fibrous substrate structure.

However, when the concentration of chitosan is more than 3 w/v%, the structure of the product is mainly in the form of flakes, and only a few of the fiber types can be intertwined to stabilize the structure of the substrate, resulting in a relatively unstable structure, and It is easy to have a flaky pattern that collapses. When the chitosan concentration is less than 0.1 w/v%, the structure of the product is mainly a fiber type. However, the fineness of the fibers makes the strength limited, resulting in a structure which is relatively unstable and easily disperses the fine fiber filaments, and it is also difficult to maintain the liquid in its structure after the liquid absorption.

In addition, the pores formed by the interdigitation of the microfibers and the sheet-like pattern in the structure can provide the ability of the substrate to absorb and moisturize the substrate, and the swelling degree of the original single fiber type can be increased from 1 to 10-15. It can solve the problem of limited absorption and moisture retention of the existing single fiber type. Similarly, when the concentration of chitosan is larger and the flaky form in the structure is increased, the gap between the structures is reduced, and the degree of swelling is also relatively reduced.

Table 1: Effect of chitosan concentration in spinning solution on chitosan fiber type.

* The method of measuring the degree of swelling is immersed in physiological test water of pH 7-7.4, and the calculation method is (the weight of the heavy-fiber-formed substrate after the fiber-formed substrate is absorbed) / the original weight of the fiber-formed substrate.

** Structural stability was measured by immersing in physiological saline overnight.

Experimental Example 2: Effect of weak alkali concentration in molding solution on chitosan fiber type

The chitosan peak molecular weight (Mp) is about 930,000 and the deacetylation degree is greater than 75%. The spinning solution was formed by dissolving 1 w/v% chitosan in a 1 w/v% aqueous lactic acid solution, and the molding liquid was an aqueous solution of Tris base having a unequal concentration. The agitating wing has a diameter of 3 cm and a stirring rate of 4000 rpm to form a chitosan fiber solution. Then, a solution containing 2 w/v% of chitosan fiber was poured into a mold, and then freeze-dried to obtain a sponge-type fibrous substrate.

From the results, it is understood that the larger the concentration of the Tris base, the thicker the chitosan fiber is, about 1-10 μm. Moreover, in the product, a small amount of a sheet-like structure is doped, but it also increases as the concentration of the Tris base increases.

When the concentration of Tris base is more than 3 w/v%, the structure of the product is mainly in the form of flakes, and only a few of the fiber types can be interlaced to stabilize the structure of the substrate, resulting in a structure that is unstable and easy to have a sheet shape. The state collapsed. The higher the concentration of the Tris base, the higher the pH of the solution, which converts the positively charged amine (NH ₃ ⁺ ) state of chitosan into an uncharged amine group (NH ₂ ), thus causing several The droplets of the chitosan are directly precipitated to form a sheet form before they have been elongated.

Conversely, when the Tris base concentration is less than 0.1 w/v%, the positively charged amine group (NH ₃ ⁺ ) of chitosan is neutralized at a slower rate, resulting in a higher rate of chitosan precipitation. Slow, so the droplets have sufficient time to be elongated to form a fiber form. However, the same is true, so that there is not enough sheet structure to stabilize the structure of the fiber substrate, and the structure of the fiber substrate is unstable, the fine fiber filaments are easily dispersed, and it is difficult to keep the liquid in the gap of the structure.

Further, as described above, since the microfibers and the sheet-like patterns are interlaced to form pores, it is possible to provide a function of absorbing and moisturizing the substrate to have a swelling degree of 10 to 16 times. However, when the concentration of the Tris base is larger, the sheet form also causes a decrease in the structure gap space and a relative decrease in the degree of swelling.

Table 2: Effect of the concentration of weak base Tris base in the molding solution on the chitosan fiber type.

* The method of measuring the degree of swelling and structural stability is the same as in Table 1.

Experimental Example 3: Effect of stirring rate on chitosan fiber type

The chitosan peak molecular weight (Mp) is about 930,000 and the deacetylation degree is greater than 75%. The spinning solution was formed by dissolving 1 w/v% chitosan in a 1 w/v% aqueous solution of lactic acid, and the molding solution was a 1 w/v% aqueous solution of tris(hydroxymethyl)aminomethane (Tris base). The agitating wings have a diameter of 3 cm and the stirring rates are not equal, forming a chitosan fiber solution. Then, a solution containing 2 w/v% of chitosan fiber was poured into a mold, and then freeze-dried to obtain a sponge-type fibrous substrate.

Table 3: Effect of stirring rate on chitosan fiber type.

* The method of measuring the degree of swelling and structural stability is the same as in Table 1.

From the results, it was found that a fiber substrate having a fiber-to-sheet shape interlaced with a diameter ranging from about 1 to 10 μm was obtained at a stirring rate of 1,000 to 8,000 rpm, and it had a liquid absorbing and moisturizing ability of 10 to 15 times. However, as the agitation rate is larger, the shear stress to which the droplets are subjected is increased, so that the diameter of the chitosan fibers is finer and the structure of the sheet form is also reduced.

When the agitation rate is greater than 8,000 rpm, the product is in the form of small fibers and flakes. The drag shear stress generated by the agitation is too large, so that the precipitated microfibers and the sheet form are sheared by high shear stress. Therefore, the structure and stability of the substrate are further affected, and the functions of aspirating and moisturizing are limited.

When the agitation rate is less than 1,000 rpm, the product structure is mainly in the form of a sheet. The drag shear stress generated by the agitation is small, and the chitosan droplets have been precipitated into a sheet form before they have been deformed and elongated. Therefore, its preferred agitation rate ranges from 1,000 to 8,000 rpm, and its preferred Reynolds number in water ranges from 1.47 x 10 ^{4 to} 1.176 x 10 ⁵ .

Experimental Example 4: Chitosan/polyethylene glycol composite fiber

The chitosan peak molecular weight (Mp) is about 930,000, the deacetylation degree is greater than 75%, and the weight average molecular weight (Mw) of the polyethylene glycol is about 4,000. In the spinning solution, the total concentration of chitosan and polyethylene glycol was 1 w/v%, and the solvent was 1 w/v% of an aqueous lactic acid solution. The molding solution was a 1 w/v% aqueous solution of Tris base. The agitating wing has a diameter of 3 cm and a stirring rate of 4,000 rpm to form a chitosan/polyethylene glycol composite fiber solution. Then, a chitosan/polyethylene glycol composite fiber solution containing 2 w/v% was poured into a mold, and then freeze-dried to obtain a sponge-type composite fiber substrate.

Table 4: Chitosan/polyethylene glycol composite fiber

* The method of measuring the degree of swelling and structural stability is the same as in Table 1.

From the results, it is understood that when the ratio of polyethylene glycol/chitosan is 0.05-0.25, the larger the proportion of polyethylene glycol, the more the structure of the sheet form. Although polyethylene glycol can be deformed and elongated together with the chitosan along with the drag shear stress, polyethylene glycol cannot precipitate in the molding liquid to form a phase change, and is still compounded in the form of mucus to the phase change precipitated. In chitosan. Therefore, after lyophilization, a composite fiber substrate of polyethylene glycol and chitosan can be produced.

The addition of polyethylene glycol can increase the swelling degree of the chitosan fiber substrate, from about 15 times to about 24 times, because polyethylene glycol has better hydrophilicity and absorption than chitosan. Liquid. However, when the proportion of polyethylene glycol added is increased, the sheet-like structure is also increased, and the gap between the fiber structures absorbing liquid can be reduced, so that the degree of swelling is also reduced. In addition, when the structure is mainly in the form of a sheet, it is impossible to stabilize the structure of the substrate by the staggered configuration between the fibers, so that the structure is unstable and easily collapsed.

Experimental Example 5: Chitosan/collagen composite fiber

The chitosan peak molecular weight (Mp) is about 930,000, the deacetylation degree is greater than 75%, and the weight average molecular weight (Mw) of the collagen from the fish scale is greater than 180,000. In the spinning solution, the total concentration of chitosan and collagen was 1 w/v%, and the solvent was 1 w/v% of an aqueous lactic acid solution. The molding solution was a 1 w/v% aqueous solution of Tris base. The stirring rate was 4,000 rpm to form a chitosan/collagen composite fiber solution. Then, a composite fiber solution containing 2 w/v% chitosan/collagen was poured into a mold, and then freeze-dried to obtain a sponge-type composite fiber substrate.

It can be seen from the results that although the fish scale collagen is electrically neutral in the acidic composite liquid environment, the negatively charged free carboxyl group (-COO ^- ) on the molecular chain and the positively charged free amine group on chitosan. (-NH ³⁺ ) electrostatically attracts, so that the fish scale collagen can undergo deformation and elongation with the chitosan together with the drag shear stress. Further, according to the degree of dry dehydration, a guanamine bond (which can be detected in the infrared (IR) vibrational spectrum) is formed, and the stability of the substrate structure is increased, so that the structure of the flaky form is only slightly increased.

When the collagen/chitosan ratio is 0.05-0.25, the addition of fish scale collagen can increase the degree of swelling of the chitosan fiber substrate to about 30 times. However, when the ratio of fish scale collagen to chitosan was 0.25, the degree of swelling decreased slightly. The number of functional groups which may be electrostatically complexed with fish scale collagen and chitosan is limited, and the fish scale collagen is water-soluble, and some of the fish scale collagen is dissolved and lost by the molding liquid, so that the degree of swelling is slightly decreased.

Table 5: Chitosan/collagen composite fiber

* The guanamine bond can be determined by IR vibration spectroscopy, and the vibration of the guanamine bond is about 1550, 1660 cm ^-1 .

** The method of measuring the degree of swelling and structural stability is the same as in Table 1.

Experimental Example 6: Chitosan/hyaluronic acid composite fiber

The chitosan peak molecular weight (Mp) is about 930,000, the deacetylation degree is greater than 75%, and the weight average molecular weight (Mw) of hyaluronic acid is greater than 300,000. In the spinning solution, the total concentration of chitosan and hyaluronic acid was 1 w/v%, and the solvent was 1 w/v% of an aqueous lactic acid solution. The molding solution was a 1 w/v% aqueous solution of Tris base. The agitating wing has a diameter of 3 cm and a stirring rate of 4,000 rpm to form a chitosan/hyaluronic acid composite fiber solution. Then, a composite fiber solution containing 2 w/v% chitosan/hyaluronic acid was poured into a mold, followed by freeze-drying to obtain a sponge-type composite fiber substrate.

From the results, it can be seen that since the free amine group (-NH ₂ ) on the chitosan can be ion-complexed with the free carboxylic acid group (-COOH) of hyaluronic acid, hyaluronic acid and chitosan can be towed together. The shear stress is deformed together and elongated. Further, according to the degree of dry dehydration, a guanamine bond (which can be detected in the infrared (IR) vibrational spectrum) is formed, and the stability of the substrate structure is increased, and the sheet-like structure is only slightly increased.

In the range of hyaluronic acid to chitosan ranging from 0.05 to 0.25, the degree of swelling of the substrate increases to about 33 times as the hyaluronic acid increases. However, when the proportion of hyaluronic acid/chitosan is increased to 0.5, hyaluronic acid and chitosan rapidly undergo ion-complexation, forming a gel and precipitating, resulting in the fluid not being able to be produced and being unable to be produced. Fiber substrate.

Table 6: Chitosan/hyaluronic acid composite fiber

* The guanamine bond can be determined by IR vibration spectroscopy, and the vibration of the guanamine bond is about 1640 cm ^-1 .

** The method of measuring the degree of swelling and structural stability is the same as in Table 1.

Experimental Example 7: Chitosan/Carboxymethyl Cellulose Composite Fiber

The chitosan peak molecular weight (Mp) is about 930,000, the deacetylation degree is more than 75%, and the carboxymethyl cellulose (CMC) has a carboxymethyl substitution degree of more than 0.8. In the spinning solution, the total concentration of chitosan and carboxymethylcellulose was 1 w/v%, and the solvent was 1 w/v% of an aqueous lactic acid solution. The molding solution was a 1 w/v% Tris base aqueous solution at a stirring rate of 4,000 rpm to form a chitosan/carboxymethylcellulose composite fiber solution. Then, a composite fiber solution containing 2 w/v% chitosan/carboxymethylcellulose was poured into a mold, followed by freeze-drying to obtain a sponge-type composite fiber substrate.

As can be seen from the results, similar to hyaluronic acid materials, chitosan has a free amine group (-NH ₂ ) which will be ion-complexed with a free carboxylic acid group (-COOH) on carboxymethyl cellulose. The chitosan is subjected to the towing shear stress together and deformed together to form an elongated precipitate. According to the degree of dry dehydration, some further form a guanamine bond (which can be detected in the infrared vibration spectrum), and the sheet-like structure is only slightly increased.

Similarly, carboxymethyl cellulose increases the degree of swelling of chitosan to about 25 times, whereas when the ratio is in the range of 0.05-0.25, the degree of swelling does not change much. It may have a limited number of functional groups that can be electrostatically complexed with carboxymethyl cellulose and chitosan. It is also possible that carboxymethylcellulose is water-soluble and has a low molecular weight, so that its solubility is high, and some components which are not combined with chitosan ions are more easily dissolved in the molding liquid and are lost. Therefore, the concentration of carboxymethylcellulose has little effect on the degree of swelling.

Table 7: Chitosan/carboxymethyl cellulose composite fiber

* The guanamine bond can be determined by IR vibration spectroscopy, and the vibration of the guanamine bond is about 1550, 1660 cm ^-1 .

** The method of measuring the degree of swelling and structural stability is the same as in Table 1.

It can be seen from the above embodiments of the present invention that not only the low-concentration weak acid weak base is used to form the chitosan fiber, but also the shearing stress generated by the stirring causes the spinning solution of the chitosan to directly solidify into the fiber in the molding liquid. . And the fiber structure is doped with a small amount of sheet-like structure, which can connect the fiber structures to increase the strength and stability of the fiber substrate structure. Next, the chitosan fibers are directly subjected to various steps of molding drying to form various types of fiber substrates. Therefore, the application of the above-described embodiments of the present invention not only solves the environmental problems of the conventional method, but also omits the non-woven process, and greatly simplifies the preparation method of the conventional fiber substrate.

Further, the obtained fibrous base material has a degree of swelling of 10 to 35 times, and various functional ingredients can be added. Therefore, it can be widely applied to a hemostatic material, a wound dressing, a tissue engineering substrate or a controlled release substrate of an active material in the medical field.

Although the present invention has been disclosed in the above embodiments, it is not intended to limit the present invention, and the present invention can be modified and modified without departing from the spirit and scope of the present invention. The scope is subject to the definition of the scope of the patent application attached.

110, 120, 130, 140. . . step

The above and other objects, features, advantages and embodiments of the present invention will become more apparent and understood.

Fig. 1 is a flow chart showing the preparation of a chitosan fiber substrate according to an embodiment of the present invention.

110, 120, 130, 140. . . step

Claims (10)

A preparation method of a chitosan fiber substrate, the preparation method comprising: preparing a spinning solution, the spinning solution comprising an aqueous solution of chitosan lactic acid, the concentration of the chitosan and the lactic acid being 0.1-2.5 respectively w/v%; preparing a molding liquid, the molding liquid comprises an aqueous solution of a weak base having a concentration of 0.1-2.5 w/v%; allowing the spinning to be dropped into the molding liquid, and rapidly stirring Allowing the spinning solution to solidify into fibers, and uniformly distributing the fibers in the molding liquid to form a chitosan fiber solution; and subjecting the chitosan fiber solution to a shape drying step to form a fiber Substrate. The method for preparing a chitosan fiber substrate according to claim 1, wherein the spinning solution further comprises a functional component, which is hyaluronic acid, carboxymethyl cellulose, alginate, poly Ethylene glycol, collagen or gelatin. The method for producing a chitosan fiber substrate according to claim 1, wherein the rapid stirring causes the Reynolds number of the molding liquid to be 1.47 × 10 ^{4 -} 1.176 × 10 ⁵ . The method for producing a chitosan fiber substrate according to claim 1, wherein the weak base comprises tris(hydroxymethyl)aminomethane. A chitosan fiber substrate prepared by the method of any one of claims 1-4. The chitosan fiber substrate according to claim 5, wherein the fibers have a diameter of 10 μm or less. The chitosan fiber substrate according to claim 5, wherein the chitosan fiber substrate is in the form of a sponge, a gel, a water gel or any combination thereof. The chitosan fiber substrate according to claim 7, wherein the sponge substrate has a degree of swelling of 10-35 times. A pharmaceutical substrate made using the chitosan fiber substrate of any one of claims 5-8. The medical substrate according to claim 9, which is a controlled release substrate of a blood material, a wound dressing, a tissue engineering substrate or an active material.