TWI384946B - Method for eliminating the poultry of low egg production rate at the specific weeks of age - Google Patents

Description

Use of avian biological indicators to eliminate low-yielding eggs and poultry at specific ages of poultry

The invention relates to a poultry screening method, in particular to a poultry egg laying rate screening method, which can select a poultry with a higher egg production rate by using an egg laying rate screening method in the early stage of cultivation, thereby improving the overall egg production rate.

In recent years, the development of animal husbandry in Taiwan has increased due to the improvement of production technology, and the demand for high-quality meat products has gradually increased. It has gradually changed from the business model of regional sideline business to the business model of capital and technology. According to statistics, in the past few years, domestic livestock products accounted for about 30% of the agricultural production value. The annual output value of the poultry industry is about 40 billion Taiwan dollars, second only to pigs and rice, making it the third largest industry in agriculture. Taiwan joined the World Trade Organization (WTO) in 2001. Poultry meat products are currently imported by tariff quotas. Since the cost of raising poultry in China is higher than that in Europe and the United States, the trade market in Western Europe in 2005 has been very open to the poultry industry. Big impact. In addition to strengthening automated production methods to reduce costs and establish a smooth production and marketing channel, it is a very important issue to improve poultry reproduction and improve agricultural competitiveness.

At present, the poultry industry is dominated by chickens, including broilers, eggs and other related industries. The annual output value of the broiler market is about 26 billion yuan, accounting for 60% of the poultry industry's output value. Since there are no original breeders in Taiwan, all the chicken species are imported from Japan, South China and other regions, and the breeding power has been declining year by year due to systematic production, labeling and selection. If traditional hybridization techniques are used to improve the genetic traits of poultry to enhance reproductive capacity, the cost is higher and the time is longer, so it is not suitable for the current situation.

Furthermore, egg production capacity is a major economic feature of poultry. In order to improve the ability to lay eggs, systematic farming methods have been adopted in poultry farming in countries around the world, and some biological indicators of poultry such as body weight, growth time, egg production rate, egg production size, and continuous egg laying period have been adopted. The time between a nest of eggs and a nest of eggs (inter-clutches) and the time of each intra-clutches, and hierarchical follicles, used to enhance the ability to raise and lay eggs. Reference indicator. However, the above-mentioned parameters for measuring the phenotypes of the egg production and the traits of the egg laying ability are generally limited to mature female birds. However, some poultry farming does not apply to the well-known systematic farming methods. In recent years, many reports have indicated that genotypes of functional genes affect variations in phenotypes. Moreover, research on genotypes and polymorphisms has become an important method to accelerate the study of trait genes in poultry populations. In general, the above genetic research methods are limited to long-term farming of poultry, not to the random breeding of poultry.

On the other hand, there are many factors that affect the sexual maturity of the birds to the laying of eggs and the rate of laying eggs, which are external breeding environment, feeding methods and intrinsic genetic traits, physiological regulation and other factors, in addition to hereditary traits, external Factors and internal physiological changes are often closely related. At present, the way chicken farmers use it more often is to use light irradiation time and nutrition to control egg production. Studies in light have found that if birds are exposed to visible light, they can stimulate the sexual maturity of birds to lay eggs in advance. However, if the complete nutritional needs are not provided during the laying and pre-production period of the poultry, even if the number of eggs laid is increased, the quality of laying eggs will be poor. The higher egg production may be caused by premature egg production or long egg laying period, although it is still unclear which factors are related to egg production rate and broodiness, regardless of premature egg production or long egg laying period, these are affected by the environment. And the interaction between hormones, hormones may play a more important role in a variety of membrane protein receptors.

At present, the domestic chicken genomics research program is aimed at screening the gene expression of high-yield egg-earth chickens. Because the low egg production rate causes the cost of the chicken industry to become too high and the competitiveness is weakening, it is hoped that through the study of functional genomics and high-yielding egg genes, and through the expression sequence (expressed sequence) Tag) method to understand the gene expression of high-yielding egg chickens in various tissues, and to use the subtractive subtractive hybridization (SSH) to develop genes related to high-yielding egg characteristics, in order to further understand and overcome the chicken The problem of low egg production rate.

Since the gene mapping of most breeds of chickens has been completely unraveled, the number of defined genes on the genetic map has also increased. In addition, when the human genetic information is gradually unraveled, the development trend of biotechnology will enter the post-genome era, and the biomarker development strategy based on the genome and protein body has gradually become the new mainstream of animal breeding technology. In order to improve the ability of poultry to lay eggs, the use of proteomic methods to increase the number of eggs or egg production has become a new choice. In the study of foreign scholars, a variety of development-related proteins have been found in the growth process of birds, and the performance of these proteins can be used as molecular markers for screening egg production ability. At present, Taiwan's aquaculture industry still lacks systematic farming methods to improve the ability of poultry to lay eggs. Therefore, while the breeding efficiency is reduced, the breeding cost is also relatively increased. Therefore, increasing the egg production rate is an important issue to stimulate competition in the aquaculture market.

At the current Taiwanese chicken market, the annual output value of the red-feathered chicken is about 1.5 million, and the black-feathered chicken has a production value of about 1 million a week. Therefore, the Taiwanese chicken market has a production value of nearly 2.5 million a week. For the Taiwanese chicken market, its market demand is quite large. Furthermore, traditional chicken farming methods have to wait until about 40 weeks of age to predict their ability to lay eggs, which is quite time consuming and inefficient. Therefore, there is an urgent need to provide a method that can increase the egg production rate and shorten the breeding time to meet the huge market demand.

The Republic of China Patent Publication No. I308929 provides an effective method for screening high-yield eggs and poultry, which is characterized by the use of insulin-like growth factor-I (IGF-I) as an index. The method comprises collecting the test sample and detecting the total amount of insulin-like growth factor-I in the test body, wherein the judgment of the high-yield egg-and-bird includes whether the insulin-like growth factor-I detected by the test body contains a relatively high amount. The present invention also provides an epitope of insulin-like growth factor-I and an antibody against the epitope.

The Republic of China Patent Publication No. 200925274 discloses a screening method for poultry egg production rate, which is a method for using a screening method to increase the egg production rate of a poultry, comprising: collecting a plurality of poultry protein samples and egg production rate information; Converted into a plurality of scores and serial numbers and corresponding passwords; combined scores and serial number passwords are regional passwords and compared to each other; and screening for the desired birds. The invention also discloses another method comprising collecting information on avian protein samples of known and unknown populations and egg production rate, sorting for comparison; selecting the desired egg production rate from a known group of poultry information; according to known ethnic groups Poultry evaluation, defining the area code; comparing the known and unknown group of poultry, selecting the poultry with the same area code from the poultry group of the unknown group and the required egg production rate; and screening the results according to the regional code matching rate The rate of egg production required for poultry.

In view of the above, the present invention utilizes the statistics disclosed in the Insulin-like Growth Factor-I (IGF-I) and the Republic of China Patent Publication No. 200925274 as disclosed in the Republic of China Patent Publication No. I308929. Methods, algorithms, password screening methods, and the use of a plurality of databases established to eliminate low-yielding laying hens at specific ages of poultry, which is a biomarker using poultry to be eliminated at specific ages of poultry Low-yield laying hen strategy. Therefore, the method provided by the present invention can predict the egg production rate of poultry as early as possible, and can achieve the effect that the prior art or general statistical methods can not be satisfied by setting a threshold value to screen the egg production rate.

It is an object of the present invention to provide a method for phasing out low-yielding egg poultry at a specific age of avian using at least one biological indicator of poultry comprising: establishing at least one database comprising at least one poultry at least for a particular week of age The concentration of a biological indicator, the ratio of the conversion of the concentration, and the calculation using a statistical method generate information corresponding to the password of the evaluation, the total number of eggs laid, and the egg production rate corresponding to the total number of eggs produced; Setting a predetermined egg production rate in a database, defining a range and/or a range of passwords corresponding to the poultry that is lower than the preset egg production rate; measuring at least one creature of the poultry to be tested at the specific week age The concentration of the indicator; the concentration of the at least one biological indicator of the poultry to be tested is converted into the above-mentioned evaluation, and the statistical method is used to calculate the password corresponding to the above evaluation; the password obtained by the poultry to be tested is in the at least one database. Performing an alignment; and if the password obtained by the poultry to be tested is within a range and/or range below the preset egg production rate, determining that the poultry to be tested is a low-yielding egg Class.

The above method for using at least one biological indicator of poultry to eliminate low-yielding egg poultry at a specific age of the poultry, wherein the biological indicators include serum proteins, insulin-like growth factor-I (IGF- I), Apolipoprotein AI (ApoA-I), vitellogenin, insulin-like growth factors (IGFs), proteins like IGF-I, proteins like IGFs, fat-deficient very low-density lipids Protein-II (Apo VLDL-II; VLDL: very low density lipoprotein), or protein related to egg production.

The above method utilizes at least one biological indicator of a poultry to eliminate low-yielding egg poultry at a specific age of the poultry, wherein the poultry includes poultry of various breeds such as chicken, duck, goose, bird, turkey, donkey, and ostrich.

Another object of the present invention is to provide a method for phasing out low-yield egg poultry at a specific age of a poultry using at least one biological indicator of a poultry, wherein the specific age includes 12 weeks, 14 weeks, 16 weeks, 18 weeks, 24 weeks. , 28 weeks, or weeks of age. Wherein, the preset egg production rate may include 60%, 50%, 40%, 30%, 20%, or an egg production rate thereof.

It is still another object of the present invention to provide a method for phasing out low-yielding egg poultry at a specific age of a poultry using at least one biological indicator of poultry, comprising: establishing a plurality of databases, wherein each database comprises at least one of a plurality of birds The concentration of at least one biological indicator at a specific age, the evaluation of the conversion of the concentration, and the calculation using a statistical method to generate a password corresponding to the evaluation, the total number of eggs laid, the egg production rate corresponding to the total number of eggs, and the like. Information; setting a predetermined egg production rate in the plurality of databases, defining a range and/or a range of passwords corresponding to the poultry below the preset egg production rate; measuring the poultry to be tested at a specific age a concentration of the at least one biological indicator; converting the concentration of the at least one biological indicator of the to-be-tested poultry into the above-mentioned evaluation, and using a statistical method to calculate a password corresponding to the above-mentioned evaluation; the password obtained by the poultry to be tested is in the plural Comparing in a database; and if the password obtained by the poultry to be tested appears in the plurality of databases below a predetermined egg production rate and/or a range of predetermined times , It is judged that the test birds avian egg is low.

The above method for utilizing at least one biological indicator of a poultry to eliminate low-yielding egg poultry at a specific age of the poultry, wherein the predetermined number of times comprises one, two, three, four, five, or any suitable number of times. The above biological indicators may include serum proteins, insulin-like growth factor-I (IGF-I), lipoprotein AI (ApoA-I), vitellogenin, insulin-like growth factors (IGFs), and similar IGF-I. Protein, protein like IGFs, Apo VLDL-II (VLDL: very low density lipoprotein), or protein related to egg production.

The above-mentioned poultry includes poultry of various breeds such as chicken, duck, goose, bird, turkey, donkey, and ostrich. The specific weeks of age described above include 12 weeks, 14 weeks, 16 weeks, 18 weeks, 24 weeks, 28 weeks, or weeks of age. Since 14 weeks of age is a critical period, which is a key decision point for determining the retention of chickens as laying hens or as edible chickens, the method provided by the present invention can correctly eliminate low-yielding laying hens at this time and retain appropriate quantities. The higher laying hens. The above predetermined egg production rate includes 60%, 50%, 40%, 30%, 20%, or the egg production rate thereof.

It is still another object of the present invention to provide a simple poultry egg laying rate screening method which utilizes the advantages of a protein analysis method to compare a specific serum protein concentration with a specific serum protein concentration of a known egg production rate in a library, if it occurs in If the interval and/or range of the preset egg production rate is less than one or a predetermined number of times, it is judged that the bird to be tested may be a low-yielding egg. In this way, the egg production rate can be effectively improved by breeding high-yield eggs and poultry, and there is no need to raise too many low-yielding eggs and poultry, which can fully save the space and funds of poultry farmers, thereby reducing costs, reducing waste of resources, and making proper use of space. It can continue to maintain high egg production rate through several generations of screening, or enhance the egg production ability of the offspring through eugenic breeding, and ultimately improve the overall competitiveness. It can also provide poultry traders and breeding researchers as an opportunity to improve the egg production rate and fertility of poultry. Reference basis, as well as the technical basis for scientific research and industrial use.

Other features and advantages of the present invention will become more apparent from the detailed description which follows. However, it is to be understood that the invention is not to be construed as a Various changes and modifications will be apparent from the detailed description of the specification.

The detailed description is to be considered in all respects as the The explicit description herein is merely illustrative of various embodiments of the invention. As stated, the specific modifications discussed herein are not intended to limit the scope of the invention. It is apparent that those skilled in the art will recognize that various changes, modifications, and alterations may be made in the equivalent embodiments of the invention.

The technical terms used in the specification are to be interpreted in their broadest sense, even if they are used in the particular embodiments of the invention.

As shown in FIG. 1, a preferred embodiment of the present invention provides a method for phasing out low-yielding egg poultry at a specific age of a poultry using at least one biological indicator of poultry, comprising: step 100, establishing at least one database, including The concentration of at least one biological indicator at least one specific age of at least one specific age, and the conversion calculated by the concentration, using statistical methods to calculate the password corresponding to the evaluation, the total number of eggs produced, and the total number of eggs produced Information such as the egg production rate; in step 102, setting a predetermined egg production rate in the at least one database, and defining a range and/or a range of passwords corresponding to the poultry that is lower than the preset egg production rate; 104. Measure the concentration of the at least one biological indicator of the poultry to be tested at the specific week age; and step 106, convert the concentration of the at least one biological indicator of the poultry to be tested into the above-mentioned evaluation, and perform a calculation using a statistical method to generate a corresponding The password of the above comparison; in step 108, the password obtained by the poultry to be tested is compared in the at least one database; and in step 110, if the password of the poultry to be tested is obtained In the range and/or range below the preset egg production rate, the poultry to be tested is judged to be a low-yield egg poultry.

The above method for utilizing at least one biological indicator of a poultry to eliminate low-yielding egg poultry at a specific age of the poultry, wherein the biological indicators may include, but are not limited to, serum proteins, insulin-like growth factor-I (insulin-like growth) factor-I, IGF-I), Apolipoprotein AI (ApoA-I), vitellogenin, insulin-like growth factor (IGFs), proteins like IGF-I, proteins like IGFs, Low-density lipoprotein-II (Apo VLDL-II; VLDL: very low density lipoprotein), or protein related to egg production.

In a preferred embodiment, the biological indicators in the above methods are insulin-like growth factor-I (IGF-I) and adefeatolipoprotein A-I (ApoA-I). Among them, the concentration of IGF-I was obtained by antibody signal analysis, and the concentration of ApoA-I was obtained by protein concentration signal analysis.

The above methods, algorithms, and password screening methods used in steps 100, 102, 106, and 108 are used in the method of using at least one biological indicator of poultry to eliminate low-yielding egg poultry at a specific age of the bird (eg, 14 weeks). For the disclosure of the Republic of China Patent Publication No. 200925274, at least one of the databases is established by the method disclosed in the Republic of China Patent Publication No. 200925274. In a preferred embodiment, the statistical method, algorithm, and password screening method used in the method of the present invention is the second embodiment disclosed in the Republic of China Patent Publication No. 200925274.

The above method for utilizing at least one biological indicator of a poultry to eliminate low-yielding egg poultry at a specific age of the poultry, wherein the poultry may include, but is not limited to, an egg-producing chicken, duck, goose, bird, turkey, donkey, ostrich Various species of poultry.

The method of the present invention utilizes at least one biological indicator of a poultry to eliminate low-yielding egg poultry at a specific age of the poultry, wherein the specific age can include, but is not limited to, 12 weeks, 14 weeks, 16 weeks, 18 weeks, 24 weeks , 28 weeks, or weeks of age, or any desired age. In a preferred embodiment, the specific week is 14 weeks. In one embodiment, the above-mentioned poultry is a Taiwanese chicken. Since the 14-week age is a critical period, the method provided by the present invention can be determined as a key timing for determining the retention of the chicken as a laying hen or as a chicken. The correct elimination of low-yielding laying hens at this time and the retention of the appropriate number of higher-yielding laying hens will not only save the space and funds of poultry farmers, but also maintain high yields in the future through several generations of screening or even through eugenics. Breeding further enhances the ability of the offspring to lay eggs and enhance the competitiveness of the industry. Since the average laying age of common chickens is 24 weeks, the strategy of the present invention to eliminate low-yield laying hens at 14 weeks of age is of great benefit to poultry farmers.

Furthermore, the method for using the at least one biological indicator of the poultry to eliminate low-yielding egg poultry at a specific age of the poultry according to the present invention, wherein the predetermined egg production rate may include, but is not limited to, 60%, 50%, 40 %, 30%, 20%, or the egg production rate thereof, or any suitable egg production rate. In a preferred embodiment, the predetermined egg production rate is set to 50%. In one embodiment, the above bird is a Taiwanese chicken. Since the preset egg production rate set in the database is higher, the larger the range and/or range of the password corresponding to the poultry that is lower than the preset egg production rate (for example, 50%), The more low-yielding eggs and poultry that are eliminated, the less the number of laying eggs that the owner retains. Therefore, according to the space and funds owned by the owner at that time, the number or percentage of eggs and poultry they want to keep can be assessed. Set the default egg production rate to screen high-yielding eggs and poultry.

As shown in FIG. 2, in a preferred embodiment of the present invention, there is provided a method for phasing out low-yielding egg poultry at a specific age of a poultry using at least one biological indicator of a poultry, comprising: step 200, establishing a plurality of databases Each of the databases includes a concentration of at least one biological indicator of the plurality of birds at least one specific week of age, a rating converted by the concentration, and a statistical method for generating a password corresponding to the rating, a total number of eggs, Corresponding to the information such as the egg production rate of the total number of eggs laid; in step 202, a predetermined egg production rate is set in the plurality of databases, and a password corresponding to the poultry that is lower than the preset egg production rate is defined. The interval and/or the range; in step 204, measuring the concentration of at least one biological indicator of the to-be-tested poultry at a specific age; and in step 206, converting the concentration of at least one biological indicator of the to-be-tested poultry into the above-mentioned evaluation, and performing calculation using a statistical method Generating a password corresponding to the above rating; step 208, comparing the password obtained by the bird to be tested in the plurality of databases; and step 210, if the bird to be tested obtains The password appears in a plurality of databases below a preset egg production rate and/or a predetermined number of times, and the poultry to be tested is judged to be a low-yielding egg.

The above method for utilizing at least one biological indicator of a poultry to eliminate low-yielding egg poultry at a specific age of the poultry, wherein the predetermined number of times comprises one, two, three, four, five, or any suitable number of times. The above biological indicators may include, but are not limited to, serum proteins, insulin-like growth factor-I (IGF-I), lipoprotein AI (ApoA-I), vitellogenin, insulin-like growth. Factor (IGFs), IGF-I-like proteins, IGFs-like proteins, Apo VLDL-II (VLDL: very low density lipoprotein), or egg-related proteins.

In a preferred embodiment, the biological indicators in the above methods are insulin-like growth factor-I (IGF-I) and adefeatolipoprotein A-I (ApoA-I). Among them, the concentration of IGF-I was obtained by antibody signal analysis, and the concentration of ApoA-I was obtained by protein concentration signal analysis.

The above methods, algorithms, and password screening methods used in steps 200, 202, 206, and 208 are used in the method of using at least one biological indicator of poultry to eliminate low-yielding egg poultry at a specific age of the bird (eg, 14 weeks). For the disclosure of the Republic of China Patent Publication No. 200925274, at least one of the databases is established by the method disclosed in the Republic of China Patent Publication No. 200925274. In a preferred embodiment, the statistical method, algorithm, and password screening method used in the method of the present invention is the second embodiment disclosed in the Republic of China Patent Publication No. 200925274.

The above method for utilizing at least one biological indicator of a poultry to eliminate low-yielding egg poultry at a specific age of the poultry, wherein the poultry may include, but is not limited to, an egg-producing chicken, duck, goose, bird, turkey, donkey, ostrich, etc. Various species of poultry.

The method of the present invention utilizes at least one biological indicator of a poultry to eliminate low-yielding egg poultry at a specific age of the poultry, wherein the specific age can include, but is not limited to, 12 weeks, 14 weeks, 16 weeks, 18 weeks, 24 weeks , 28 weeks, or weeks of age, or any desired age.

Figure 3 shows three screening modes for the use of at least one biological indicator of poultry to eliminate low-yielding egg poultry at a specific age of poultry in an embodiment of the present invention: After Egg, Twice Egg, Uni Egg, wherein The databanks are: A, B, and C. In one embodiment, insulin-like growth factor-I (IGF-I) and lipoprotein AI (ApoA-I) are used as biomarkers, After Egg: concentration of IGF-I and ApoA-I in the poultry to be tested The password calculated by the algorithm appears in the range or range of the egg production rate in the database A or B or C less than 50%, that is, the poultry with a lower egg production rate of less than 50% in the database A or B or C. The concentration or range of the IGF-I and ApoA-I concentrations calculated by the above algorithm is predicted to be eliminated by the low egg production rate of birds; Twice Egg: if the IGF-I of the poultry to be tested And the concentration of ApoA-I calculated by the above algorithm appears in the three databases in the interval or range of less than 50% of the egg production rate, and it is predicted to be eliminated by the low egg production rate of birds; Uni Egg: If the concentration of IGF-I and ApoA-I in the poultry to be tested is calculated by the above algorithm, as long as it appears in the interval or range of the egg production rate of less than 50% in the three databases, it is predicted to be The poultry with low egg production rate is eliminated.

Figure 4, Figure 5, and Figure 6 show the results of elimination using the three screening modes in Figure 3. In a preferred embodiment, the selected birds are Taiwanese chickens, and the default egg production rate is set to 50%. The database is three: A, B, C. In one embodiment, screening is performed in a single database (screening mode: After Egg), and if the concentration of IGF-I and ApoA-I of the chicken to be tested is generated by the above algorithm, the password is generated in the database. The interval or range in which the rate is less than 50%, that is, the range or range in which the concentrations of IGF-I and ApoA-I of the chickens whose egg production rate is less than 50% in the database are calculated by the above algorithm. It is estimated that it may be a chicken with a lower egg production rate. Since the database already contains the concentration of a plurality of biological indicators (such as IGF-I, ApoA-I, etc.) at a specific age (such as 14 weeks, 16 weeks, 18 weeks, etc.), the conversion of the concentration is converted. Using statistical methods to calculate the number of passwords corresponding to the evaluation, the total number of eggs laid, and the egg production rate corresponding to the total number of eggs produced, so the password and the database of the chickens are measured. The password can predict the future egg production rate of the chicken in the early stage (such as 14 weeks). In one embodiment, as shown in FIG. 4, the to-be-tested birds to be tested are forty 14-year-old Taiwanese chickens, which are screened by a single database A, and the preset egg production rate is set to 50%. The elimination rate of the chickens to be tested is 12.5%. The average number of eggs laid by the remaining chickens after the elimination is increased from 82 to 83. The single database B is used for screening. The default egg production rate is set at 50%. After the comparison, the elimination rate of the chickens to be tested was 15%. After the elimination, the average number of eggs laid by the remaining chickens was increased from 82 to 83; the single database C was used for screening, and the default egg production rate was set at 50%. Then, the elimination rate of the chickens to be tested after the comparison was 20%, and the average number of eggs laid by the remaining chickens after the elimination was increased from 82 to 87.

In another embodiment, as shown in FIG. 4, the to-be-tested birds to be tested are forty 14-week-old Taiwanese chickens, which are screened by three databases (A, B, and C), and the eggs are preset. The rate is set to 50%. If the concentration of IGF-I and ApoA-I of the chicken to be tested is calculated by the above algorithm, the passwords appear in the three databases and the egg production rate is less than 50% or the range is twice. (Screening mode: Twice Egg), if it is judged to be low-yielding laying hens, the elimination rate of the chickens to be tested after the comparison of the three databases is 15%, and the average number of eggs laid by the remaining chickens after the elimination is 82 were upgraded to 84.

In a preferred embodiment, as shown in FIG. 4, the to-be-tested poultry to be tested is forty 14-year-old Taiwanese chickens, which are screened by three databases (A, B, and C). The egg rate is set to 50%. If the concentration of IGF-I and ApoA-I of the chicken to be tested is calculated by the above algorithm, the password will appear in the interval or range of the egg production rate of less than 50% in the three databases. (Screening mode: Uni Egg), if it is judged to be low-yielding laying hens, the elimination rate of the chickens to be tested after the comparison of the three databases is 30%, and the average number of eggs laid by the remaining chickens after the elimination is The number of 82 eggs increased to 87, and the average number of eggs laid was significantly improved.

As shown in FIG. 5, in one embodiment, the selected poultry is forty Taiwanese chickens of 16 weeks old, the preset egg production rate is set to 50%, and the selected database is three: A, B, C. . The screening strategy is the same as that described in the 14-week-old method, and is screened by a single database (A, B, or C) (screening mode: After Egg), or in three databases, the egg production rate is less than 50%. The interval or range is twice (screening mode: Twice Egg) or once (screening mode: Uni Egg) as the screening standard, but as shown in the data in Figure 5, the results of the screening of Taiwanese chickens at the age of 16 weeks by the above method are not Significantly at 14 weeks of age, the best average number of eggs laid after elimination was increased from 82 to 84.

As shown in Fig. 6, in one embodiment, the selected birds are forty 18-week-old Taiwanese chickens, the default egg production rate is set to 50%, and the selected database is three: A, B, and C. The screening strategy is the same as that described in the previous 14 and 16 weeks of age, and is screened by a single database (A, B, or C) (screening mode: After Egg), or in three databases, the rate of egg production is lower than 50% of the interval or range of two (screening mode: Twice Egg) or one time (screening mode: Uni Egg) as the screening criteria, as can be seen from the data in Figure 6, the most significant is a single database (A, B, or C) For screening (screening mode: After Egg), the elimination rate of the chickens to be tested after screening in a single database B is 22.5%, and the average number of eggs laid by the remaining chickens after the elimination is increased from 82 to 84. One.

At 14 weeks of age, it is a critical period for the chickens to determine the retention of the chickens as a key point for laying hens or serving chickens. The method provided by the present invention can correctly eliminate lower laying eggs at this time. Chickens and the proper number of higher laying hens can save the space and funding of poultry farmers. Therefore, for Taiwanese chicken breeders, 14 weeks of age is a very suitable period for screening.

The parameters in the above methods, the screening criteria, the biological indicators used, the specific age, the preset egg production rate and other parameters are for illustrative purposes only, and are not intended to limit the spirit of the present invention; To be applicable to one of the examples of the present invention, it is not representative of the only applicable bird, and the present invention can also be applied to various breeds of poultry such as laying hens, ducks, geese, birds, turkeys, quails, and ostriches. Using the statistical methods, algorithms, and password screening methods disclosed in the Republic of China Patent Publication No. 200925274, and the methods provided by the present invention, a database of various poultry can be established, and data of field trials can be used to find each The poultry is suitable for the specific period of screening, the biological indicators suitable for selection, etc., so that the poultry farmers can remove the cyanine, and carry out systematic and reliable screening, leaving the birds with higher average egg production.

In one embodiment of the present invention, it can be seen from Figure 7 that the concentrations of insulin-like growth factor-I (IGF-I) and lipoprotein AI (ApoA-I) have no correlation until 18 weeks until 18 weeks. IGF-I begins to show a significant positive correlation, while ApoA-I needs to see a negative correlation until 24 weeks (the laying period), so the novel method provided by the preferred embodiment of the present invention utilizes only IGF- I and ApoA-I can predict the ability to lay eggs in the early 14 weeks, which is beyond the effectiveness of the general statistical methods. As shown in Figure 8, insulin-growth factor-I (IGF-I) and lipoprotein AI (ApoA-I) and total production in Taiwanese chickens at 14 weeks, 16 weeks, 18 weeks, 24 weeks, and 28 weeks. The positive or negative correlation of the number of eggs (R: Pearson coefficient of correlation) is very low, can not be directly judged or by setting a threshold to screen the egg production rate, however, when using the method provided by the present invention, It is possible to predict the likely tendency of the egg production rate in a group of people at a specific age (eg, 14 weeks) by comparison with the database, that is, whether it is higher or lower than the preset egg production rate, and whether it tends to be eliminated. Therefore, the novel methods provided by the present invention will be highly anticipated regardless of whether they are applied to the industry or academia.

While the present invention has been described in terms of the specific embodiments thereof, many modifications and changes can be made without departing from the spirit and scope of the invention. Therefore, the scope of the present invention is limited only by the "claimed patent scope" and equivalents thereof which will be described later.

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The drawings illustrate the preferred embodiments of the invention, which are intended to be illustrative only and not to limit the scope of the invention.

1 is a schematic flow chart of a method for using at least one biological indicator of poultry to eliminate low-yielding eggs and poultry at a specific age of poultry according to an embodiment of the present invention;

2 is a schematic flow chart of a method for using at least one biological indicator of poultry to eliminate low-yielding egg poultry at a specific age of poultry according to a preferred embodiment of the present invention;

Figure 3 is a three screening mode for using the at least one biological indicator of the poultry to eliminate low-yielding egg poultry at a specific age of the poultry in the embodiment of the present invention, wherein the database used is: A, B, C;

Figure 4 is a diagram showing the elimination rate of the low-yielding egg poultry at the age of 14 weeks using the three screening modes in Figure 3, using the three screening modes in Figure 3, and the elimination rate after the elimination of the concentration of IGF-I and ApoA-I in Taiwanese chickens. The number of eggs laid, the database used is: A, B, C;

Figure 5 is a diagram showing the elimination rate of the low-yield egg poultry at the age of 16 weeks and the egg production after the elimination of the concentration of the IGF-I and ApoA-I in Taiwan using the three screening modes in the third embodiment of the present invention. Number, wherein the database used is: A, B, C;

Figure 6 is a diagram showing the elimination rate of low-yield eggs and poultry at the age of 18 weeks using the three screening modes in Figure 3, using the three screening modes in Figure 3, and the eggs after elimination. Number, wherein the database used is: A, B, C;

Figure 7 is a graph showing the relationship between the age of a plurality of Taiwanese chickens and the average concentration of IGF-I and ApoA-I in one embodiment of the present invention;

Figure 8 is a correlation between the average concentration of IGF-I and ApoA-I and the total number of eggs (R: Pearson correlation coefficient) of a plurality of Taiwanese chickens at different ages in one embodiment of the present invention.

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Claims (17)

A method for utilizing at least one biological indicator of a poultry to eliminate low-yielding egg poultry at a specific age of a poultry comprises: establishing at least one database comprising a concentration of the at least one biological indicator of the plurality of birds at the particular week age, The evaluation of the conversion of the concentration and the calculation using the statistical method generate information corresponding to the password of the evaluation, the total number of eggs laid, and the egg production rate corresponding to the total number of eggs laid; setting a pre-set in the at least one database Setting an egg production rate, defining a range and/or a range of passwords corresponding to the poultry that is lower than the preset egg production rate; measuring the concentration of the at least one biological indicator of the test poultry at the specific week age; Measuring the concentration of the at least one biological indicator of the poultry into the evaluation, and using a statistical method to calculate the password corresponding to the evaluation; comparing the password obtained by the poultry to be tested in the at least one database And if the password obtained by the poultry to be tested is below the range and/or the range of the preset egg production rate, it is determined that the poultry to be tested is a low-yielding egg poultry. The method of claim 1, wherein the at least one biological indicator comprises serum proteins by using at least one biological indicator of the bird to cull the low-yielding egg poultry at a specific age of the bird. The method of claim 1, wherein the at least one biological indicator comprises an insulin-like growth factor-I, wherein the at least one biological indicator comprises a low-yield egg-like bird at a specific age of the bird. IGF-I), Apolipoprotein AI (ApoA-I), vitellogenin, insulin-like growth factors (IGFs), proteins like IGF-I, proteins like IGFs, very low fat deficiency Density lipoprotein-II (Apo VLDL-II; VLDL: very low density lipoprotein), or protein associated with egg production. The method of claim 1, wherein the at least one biological indicator comprises an insulin-like growth factor-I, wherein the at least one biological indicator comprises a low-yield egg-like bird at a specific age of the bird. IGF-I), and Apolipoprotein AI (ApoA-I). The method of claim 1 for using at least one biological indicator of a poultry to eliminate low-yielding egg poultry at a specific age of the poultry, wherein the poultry is an egg-producing chicken, duck, goose, bird, turkey, donkey, ostrich . The method of claim 1, wherein the at least one biological indicator of the bird is used to eliminate the low-yielding egg poultry at a specific age of the poultry, wherein the specific week is 14 weeks. Use at least one biological indicator of poultry as described in claim 1 for poultry A method of phasing out low-yielding egg birds at a specific age, wherein the specific weeks are 12 weeks, 14 weeks, 16 weeks, 18 weeks, 24 weeks, and 28 weeks. The method of claim 1 for utilizing at least one biological indicator of a poultry to eliminate low-yielding egg poultry at a specific age of the poultry, wherein the predetermined egg production rate is 60%, 50%, 40%, 30%, 20% . A method for utilizing at least one biological indicator of a poultry to eliminate low-yielding eggs and poultry at a specific age of a poultry comprises: establishing a plurality of databases, wherein each of the plurality of poultry includes at least one biological indicator at a specific age of the bird The concentration, the ratio of the conversion of the concentration, and the calculation using a statistical method to generate information corresponding to the password of the evaluation, the total number of eggs laid, and the egg production rate corresponding to the total number of eggs produced; in the plurality of databases Setting a predetermined egg production rate, defining a range and/or a range of passwords corresponding to the poultry below the preset egg production rate; measuring the concentration of the at least one biological indicator of the test poultry at the specific week age Converting the concentration of the at least one biological indicator of the poultry to be tested into the evaluation, and performing a calculation using a statistical method to generate the password corresponding to the evaluation; the password obtained by the poultry to be tested is in the plurality of databases Performing an alignment; and if the password obtained by the poultry to be tested appears in the plurality of databases If the interval is lower than the preset egg production rate and/or the range is a predetermined number of times, the poultry to be tested is judged to be a low-yielding egg. The method for using the at least one biological indicator of the poultry to eliminate the low-yielding egg poultry at a specific age of the poultry as described in claim 9, wherein the predetermined number of times is one time, two times, three times, four times, five times. The method of claim 9, wherein at least one biological indicator is used to eliminate low-yielding egg poultry at a specific age of the bird, wherein the at least one biological indicator comprises serum proteins. The method of claim 9 for utilizing at least one biological indicator of a bird to eliminate low-yielding egg poultry at a specific age of the bird, wherein the at least one biological indicator comprises insulin-like growth factor-I (I. IGF-I), Apolipoprotein AI (ApoA-I), vitellogenin, insulin-like growth factors (IGFs), proteins like IGF-I, proteins like IGFs, very low fat deficiency Density lipoprotein-II (Apo VLDL-II; VLDL: very low density lipoprotein), or protein associated with egg production. The method of claim 9 for utilizing at least one biological indicator of a bird to eliminate low-yielding egg poultry at a specific age of the bird, wherein the at least one biological indicator comprises insulin-like growth factor-I (I. IGF-I) and Apolipoprotein A-I, ApoA-I). The method of claim 9, wherein at least one biological indicator of the bird is used to eliminate low-yielding egg poultry at a specific age of the poultry, wherein the poultry is an egg-producing chicken, duck, goose, bird, turkey, donkey, ostrich . The method of using at least one biological indicator of a poultry to eliminate low-yielding egg poultry at a specific age of the poultry as described in claim 9, wherein the specific week is 14 weeks. The method for using the at least one biological indicator of the poultry to eliminate the low-yielding egg poultry at a specific age of the poultry as described in claim 9, wherein the specific age is 12 weeks, 14 weeks, 16 weeks, 18 weeks, 24 weeks, 28 week. The method for using the at least one biological indicator of the poultry to eliminate the low-yielding egg poultry at a specific age of the poultry as described in claim 9, wherein the predetermined egg production rate is 60%, 50%, 40%, 30%, 20% .