TWI375795B - Method for determining the geographic origin of koshihikari cultivated in taiwan and foreign countries by appearance properties, chemical components, and physicochemical properties - Google Patents

Description

1375795 IX. Description of the invention: [Technical field to which the invention pertains] ηίΓ The discriminating method, in particular, relates to the appearance of a straw from Koshihikari white red and the physical and chemical properties, and the Koshihikari of the rice producing area is green. Da is the first white [prior art]

The private list or the variety of agricultural products or foods are often recognized and favored by the consumer market, and can be the first in the highly competitive market, and the pot is higher than the rice frequency in the rice market. The s-boat is the important factor in determining the badness of t-filament, and the K-Min is the highest-priced product in Taiwan's current rice market. The Koshihikari rice has the highest value, and its price is mostly higher than the price of Taiwan's f-meter rice. For this reason, in order to attract the eyes of the people to seek high, the sales volume and the high-level, the robe products are based on the origin of the fake stomach or doped with the non-marked origin of the rice (four) event layer is poor, resulting in the benefit of the box Damaged, consumers also spent more money to buy unreal goods. In the modernization of inspection technology, sub-inspection can be used to identify varieties, but it is impossible to distinguish the production areas. In addition, 'Taiwan-related verification units such as the Food Industry Development Research Institute cannot provide effective production areas _test' and even if they provide care (farmers, The dish rice factory and the rice merchants have their own certificate of origin. They are also unable to gain the trust of consumers (the general public and the general public). It is known that the establishment of the rice origination technology is becoming more and more urgent and important. In general, the product of Linshi Peiwen includes lion element content analysis or stable isotope content analysis. However, due to the nature of crop cultivation environment, climate, water, soil and other methods of cultivation, it will affect the absorption of crops. Migration 1375795 and the ability to accumulate mineral S and isotope content, the content of the hormone will be affected by the external environment ^, also = farm =:: = lower surgery: tune:: _ application will be - or stable isotope content determination Early crop production: The misjudgment rate caused by the work cannot achieve the result of the accuracy of the accuracy of the plant. It is bound to be the purpose of the helmet area. Therefore, a (four) Γΐ business letter to the Red Cross will cause doubts and business interests, and build the urgency. In the rice production area, the method of arranging fortune is to be squandered [inventory content] In order to avoid the counterfeiting of the origin of the rice in the market, the consumer is deceived, and the problem of the high-level misunderstanding of the rice producing area is solved _ For _ outside and Koshihikari white rice: Strictly utilize the appearance traits and physicochemical properties of Koshihikari white-&&&&&&&&> purpose. In order to achieve the above object, the present invention provides a method for determining the production area by utilizing the appearance traits and physicochemical properties of Koshihikari rice, and the method includes the following steps: (1) measuring the length of the various known areas of Koshihikari rice, rice grains Width and physicochemical properties, the statistical analysis including the principal component analysis (Prindpala) m_entAnaiysis) _ Discriminant Analysis (Discriminant Analysis) - including the known areas of the lighter white, rice grain length, rice grain width And the physicochemical properties of the statistics _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ The values of the white rice and the steps (1) are known to describe the values, such as the production of the Guangguang white rice, the II Γ, the western part of Taiwan or the country outside Taiwan; The content of free amylose (amylGse), total nitrogen (fonit. (four) content, storage protein content and viscosity (viscQsijy) is the length of the group t for each dawn (four) green to the bottom The longest distance X grain width is the longest distance from the back of the rice grain of the Koshihikari rice to the abdomen of the rice grain ' ^ Tibetan egg self-quality content domain-level egg from f content, alcohol soluble protein _amin) and acid Or the content of the alkali-soluble protein and the content of the alcohol-soluble protein, which are obtained by adding the total storage protein (such as st〇ragepr〇tdn), wherein the acid or alkali-soluble protein can be divided into shouting or alkali-soluble protein f (a coffee). Both) and Chen or Mutelin. Compared with the previous case, only the use of mineral elements or stable isotope content to determine the domestic and foreign rice production areas, the method of the present invention is based on the appearance and physicochemical properties of rice. The factor system includes the length and width of Kumbai white rice and its physical and chemical properties, which greatly improves the accuracy of the determination of the production area of Koshihikari rice, so that the false positive rate approaches zero; in addition, the method of the present invention is based on the high unit price of Koshi white rice. The target can be accurately judged without false positive rate, and can distinguish between domestic and foreign and domestic and foreign Yueguang white rice producing areas. For the increasingly complex items in the domestic Koshihikari rice market, this method can effectively test the source of Koshihikari white rice. And the marketable Koshihikari rice doping situation, in order to provide a precise and objective proof of discrimination between rice suppliers and consumers, help to ensure the market segmentation and inclination of rice in China, according to the method can be used as other scales The basis of technology research and development. The embodiments of the present invention will be further described with reference to the drawings, which are set forth to illustrate the invention, and are not intended to limit the scope of the invention. Within the spirit and scope, Tian can make some changes and refinements. Therefore, the scope of protection of the present invention is subject to the definition of the scope of the patent application. [Embodiment] First, S1G is collected from a sample of known production area, and the sample contains 15 pieces of foreign Koshihikari rice (imported from the United States, Japan and Japan) and 10 pieces of Koshihikari white rice produced by Taiwanese East and West. The samples collected by the invention and their origin are shown in Table 1. J1. The Koshihikari white rice sample collected in the embodiment of the present invention and its production property ^_______ brand name ____ 曰 新 新 新 新 新 新 新 2、 2、 2、 2、 2、 2、 2、 2、 2、 2、 2、 2、 2、 2、 2、 2、 、 、 、 、 、 、 、 、 、 、 、 、 、 Light Australia glazing glory*2, glazing organic Koshihikari, unified organic Koshihikari, unified selection of vitality Koshihikari United States Shin Sui Koshihide, California Pure Japan Koshihika, California Koshihikari*2 'Zhongxing California Kuangguang Outside domestic Taiwan Mifu God, Mengtian Yueguang, Chishang is now collecting Yueguang, Chen Xiehe and the best products Guangguang, the bridge scale _ ton Yueguang*2, the bridge brand tastes, Lanyang five farm rice, Zhongxing Yueguang, Shanshui Yueguang - I__^Bay Features Rice-Second Forest Production*2 indicates that there are two samples. Please refer to the first figure, which is a private diagram of the 々IL of the Koshihikari rice production area in the embodiment of the present invention. Firstly, the appearance characteristics of the rice grains of the Koshihikari rice samples were measured, and the amylose content of the Koshihikari rice samples was stored as the content of the storage protein and the total nitrogen (_nitrogen) content. And viscous properties (visc〇sity) and other physical and chemical properties of 1375795 qualitative analysis s^o, in order to establish the traits of the traits and physical and chemical properties of the known production areas of Koshihikari white rice's appearance traits and physical and chemical properties The numerical value is analyzed by statistical analysis to establish a discrimination pattern S30 of the Guangguang white rice production area. When it is desired to confirm the production area of the side Guangguang white rice, the appearance traits and physical and chemical properties of the rice grains to be tested can be analyzed, and the values are input. People smothered the money to compare S5G, and then sold out the production area of the Koshihikari rice to be tested abroad, _ east or western China (10). The foregoing embodiments are described in detail as follows: Example 1: Analysis of appearance characteristics of rice grains of Koshihikari rice In addition to the external cultivation of the production area, the quality of rice in the specific production area is also affected by the cultivation of human beings. Therefore, in the search for stable agronomic traits that can be substituted for the characteristics of white and rice, it is necessary to consider the impact of these two factors, based on this 'this day is contrary to the test _ selected - (four) can be used to discriminate the origin 'and will not Correct cultivation method special cause shadow _ has appearance traits. Seeing thousands of waters

Referring to the second drawing, a schematic diagram of the lighter from the meter H of the embodiment of the present invention, the length of the rice grain i is the longest distance from the bottom 10 of the rice grain to the tip of the rice grain 2, and the width of the rice grain is from the rice (four) part 12 to The longest distance of the abdomen and the 2G grain of the above-mentioned sample, the Lilin ruler is based on the length and width of the width of the width of the wire, the average value of the wire, and the ratio of the length of the difference to the width of the width. (4) Jun, scuttle, record === 1375795 ΙΙΌ-Η99Ί lro-Ηεη 0ιό-η69ί^ο-ΗΓΛνο.»—) 卜0.0-Ηιζ/Ι οοοό-η寸/; I is. 9ΗΙ οοιό-η(ν6 (ν6(No-Htoo> ζ-ΙΌ-Ηεζ/tNcnCN.o-HON'o·inch CNIO-HS6(N(N(NO-H6Z; inch oloTs3<N6IoTs_ inch siCNooCN§-H§§.inch(εε) ) (mm)~£6

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First, weighed 1 〇〇mg ± 2 mg of each of the previously prepared Koshihikari rice samples of known origin, and placed them in a 100 mL dosing bottle. In the sample of bribes, add i to say steaming water, then add 9 mL of 1N sodium hydroxide (Na〇H), shake it for 9 minutes, and then add steam to 100 mL. Next, the paste analysis instrument (10) shirt; model: Astona2) detects the amylose content, because the straight line powder can react with iodine to produce a blue compound (Amy1〇se_I〇dine c〇mplex) in the range of ρΗ4·5~74, The blue compound was detected at a wavelength of 600 nm, and the amylose content (%, w/w) was converted from the measured blue compound content. The results are shown in Table 4. Table 4, Analysis results of the average linear powder content of Yueguang white rice at home and abroad Origin of foreign rice 15.296 ±1.5621 14.070± 1.5350 —0 Western $-----13.533 ±1.9729__ By this, township. It can be seen that the foreign rice has the highest content of the linear chain, followed by the domestic eastern rice, and the domestic west. The glutinous rice is the least 'the amylose content of the white rice in each of the producing areas can be 12 1375795. The difference protein protein content analysis and the total nitrogen content analysis dissolve the β eight parts to store protein. Forms exist, storage protein dependence, cold. Can be divided into water-soluble protein (albumjiis), 趟 soluble protein and acid or amphoteric protein Γ white Γ 二 二 二 乍 乍 乍 乍 乍 乍 乍 乍 乍 乍 乍 最 最 最 最Sexual protein, and the phase _ or alkaline egg (four) is the most important 峨 New 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于 由于The content of the alcoholic protein__ eggs (4) of the aforementioned Koshihikari rice samples, and the total storage protein content by the sum of the alcohol soluble protein and the acid or alkali soluble protein content, and the total nitrogen content of the _ Yue rice sample The results of the analysis of the measured protein content were confirmed by the results of the analysis of protein and electrophoresis. (1) Analysis of total nitrogen content of Koshihikari white rice Firstly, the above-mentioned polished Koshihikari rice samples were weighed separately and digested with sulfuric acid and oxidized wire at 38 〇t for 5 hours, in which the sample would be in the sample. Free ammonia and organic nitrogen compounds are converted into sulfate. Subsequent addition of a field buffer allows the ammonia to be oxidized by ammonium hypochlorite to ammonium chloride, which is then reacted with salicylic acid to form a salicylic acid analog of indophenol blue. The resulting water-acid analog can be detected at a wavelength of 660 rnn, and the measured absorbance at that wavelength can be converted to ammonia content, thereby measuring the total nitrogen content of Koshihikari rice. 13 1375795 The Koshihikari white rice sample and the blank sample are placed in the proportions listed in the table below and placed in a sterile nitrification tube. Place the nitrifying tube on the digestive rack, first at 16 〇. Heating under the armpit to white dioxide

Sulfur (S〇2) gas appears (about 90 minutes). Then heat up to 380. (: until the solution is transparent or light yellow, heat for another 30 minutes, the whole process takes about 5 hours. When it is slightly cooled after digestion, immediately join

Approximately 5 to 10 mL of steam veins to prevent crystal formation. Cool to room temperature and release to 75 mL without ammonia.

After the dilution is filtered, the instrument can be applied to the instrument (AstoriaAnaly^7^7^i^) to obtain the Koshihikari self-heteropurine content (%), and the silk is as shown in Table 5. • (2) The storage protein of Koshiwon white rice The content analysis of the silk before the extraction of Weiguang from the green product contains the total egg from f, and then through the protein electrophoresis Qingquan job secret egg self-quality no miscellaneous egg self-quality #. Please refer to the third figure 'this figure is the date of this The results of the protein electrophoresis analysis of the samples from the silk samples of the monthly embodiment, wherein the rice samples were produced from Changhua (wmw8), Miaoliyang 4), Australia _ and NK10) and __8) Protein strips below _d) The aliquots of the _ white recordings of the county are from f, and the post-rice or _-proteins contain two subunits, ie, the alpha in the third graph with a molecular weight of kDa _ Acid or alkali soluble protein (4) This (4) and molecular weight is about m milk peak acid or gluten protein (β-glutdin). Then, quantitative software, such as Sci〇n, is used to estimate the protein bands of the colloids, and the protein amount is compared with the number of the stalks of the stalks No. 9 (), and then the analysis is performed. 9 醇 杂 自 自 ) ) 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 、 以及 以及 以及 以及 以及 以及As shown in the series, the sister of the 9th is a cultivator of the genus of the genus, and it is the genus of Taiwanese _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ References for protein content of other Koshi white rice samples. Total nitrogen content (%) α-acid or test soluble protein (%) β-acid or test soluble protein (%) Alcohol soluble protein (%) Total storage protein ( %) 5.784 ±0.4505 0.998 ±〇.〇994 0.991 ±〇.〇886 0.945 ±0.2586 0.981 ±0.1275 6.762 ± 0.3708 6.399 ±0.4354 1.171 ±0.1512 1.144±0.〇993 1.110 ± 0.1217 1.085 ±0.0244 1.223 ±0.3441 1.260 ±0.2238 1.157 ± 0.1839 1.147 士0.0912 Table 5 shows the sample of Koshihikari white rice... Or ^^·^ : addition or reduction, that is, the accumulation of protein f content and total nitrogen content trend; and from the fruit, it is known that foreign rice has no _ egg content, thief-containing eggs or self-quality content or 15 1375795 Alcohol-soluble eggs have their own content. _ East, Western rice, their towels, Inter and National Games, chaotic slaves from the fine 峨ςΐ = 虿 具 可 可 。 。 贝 贝 贝 贝 贝Analysis of the viscosity properties of light white rice. The former Weiguang white rice sample is first measured by water content, and then ground into powder and sieved with the hole diameter of 5 holes. The secret weight is the sample plus heart g steaming hall. Water, then (Rapid ViscoAnalyzer; RVA, Newport

Sdentifielnstrunients'WarriewoM Australia) 'Detecting the viscosity change of these Koshihikari rice samples during the pasting process', each time the sample is measured, the conditions must be corrected first. The conditions for the viscosity analysis of the instrument are as follows: Stage (Stage Temperature/speed STD1 1 50°C 0 min, 0 sec 2 960 rpm 0 min, 0 sec 3 160 rpm O min, 0 sec 4 50°C 1 min, 0 sec 5 95〇C 4 min, 42 sec 6 95 〇C 7 min, 12 sec 7 50°C 11 min, 0 sec End of test 13 min, 0 sec Time between reading 4 sec and measuring the peak viscosity of the sample, strange The holding strength viscosity, the viscosity viscosity, the final viscosity, and the setbackviscosity, wherein when the temperature is higher than the paste temperature, the particles are continuously swelled and accompanied by The solubility of soluble soluble powder, so the viscosity increases rapidly, 1375795 and the highest viscosity value before the end of high temperature cooking in the viscosity curve is the peak viscosity, which is the viscosity caused by the swelling of the particles. The balance point between the granule and the granule to be degraded and the viscosity is decreased, which indicates the water_binding capacity of the starch, and can be used as the judgment of the resistance of the starch granule to the rupture ability and the dissolution of the amylopectin; the temperature-intensity viscosity is the viscosity curve. Cooling process (by 95. (: cooling to 50. (:, and at 50T: for a period of time) the lowest viscosity, the difference between the viscosity of the viscosity curve and the strange temperature, called the viscosity cracking value, generally Also known as shear-thining; the final viscosity is the viscosity value of the cooling end point after starch gelatinization; the difference between the final viscosity and the constant temperature strength viscosity is called the viscosity rise value, when the temperature drops, the starch intermolecular It will be rearranged, and the hydrogen bond between the straight amylopectin molecules in the starch will form a hydrogen bond and agglomerate, so that the viscosity will rise again, and the viscosity recovery value will be higher, indicating that the higher the re-coagulation ability of the powder, the final measurement of each white rice sample. The viscosity value (cP) is shown in Table 6. Table 6. Viscosity analysis results of Koshihikari white rice at home and abroad Physicochemical properties 邋扪 Foreign rice Taiwan East Rice Taiwan Western rice peak viscosity (cP) 49 63.530 ±392.1109 4713.600 ±414.5519 5119.400 ±9913215 Constant temperature strength viscosity (cP;) 2929.870 ±738.6026 2279.400 ± 224.6682 2967.400 ± 1159.7500 Final viscosity (cP) 3023.870 ± 742.4808 3541.200 ± 141.8792 3139.000 ±517.6592 Viscosity cracking value (cP) 2033.670 ±696.2807 2434.200 ± 390.1541 2152.000 ±555.4111 Viscosity recovery value (cP) (1939.670) ± 821.8311 (1172.400) ±390.7113 (1980.400) ± 1064.4800 The results in the table show that the viscosity values of foreign rice and domestic East and West rice in each stage of the paste formation process They are all different, showing that the viscosity of the rice grains of different regions is different. Therefore, the viscosity values of these samples can be used as another identification parameter for distinguishing the domestic and foreign origins of Koshihikari rice and the origin of the East and West regions of the country. Example 3: Establishment of the method for discriminating Koshihikari rice production area The appearance of the Koshihikari white rice samples and the analytical values of the physical and chemical properties are analyzed using a statistical analysis system such as SAS 9.1. Analysis (Principa! c〇mp〇nent such as coffee) and Discriminant analysis (Discriminant analysis) statistical analysis. L Principal Component Analysis: Principal component analysis is a multivariate data analysis method that uses less variables to distinguish the differences between the original data' can be imagined as multiple variable data sets in multidimensional space. The rigid rotation 'forms the i-th principal component in the direction with the largest variance. Orthogonal to the principal component, the second principal component is formed along the direction of the second largest variance, and so on, and finally a plurality of new variables are formed. The variable set consisting of the new variables absorbs all the information and variance of the original variable set. The third main component can express the highest variation, the second principal component, and so on. Since most of the variables 卩 验 此 此 侧 ' ' ' ' 域 域 域 域 域 域 域 域 域 域 域 域 域 ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' ' The analysis method can simplify the dimension of multivariate data. Under the premise of losing the minimum amount of information, the 'reduction of the number of variables' can observe the highest amount of information in the least dimension, without observing all the variant items in the original data. '" In the embodiment of the present invention, the above-mentioned rice grain length and width traits and the physicochemical properties were subjected to principal analysis. The analysis results are shown in Table 7. 1375795 Table 7 Principal component analysis results of rice grain length and width traits and physicochemical properties of domestic and foreign Koshihikari rice No. 1 2nd 4th 5th 6th 7th Characteristic '% variation percentage (%) Product variation percentage 4.4331 .31.66% 31.66 % 3.9080 27.91% 59*58% soil loss VT 23990 17.14% 76.71% Wang Feng W 12309 &79% 8551% Wang Chengwu ' 0Λ333 5.95% 91.46% Wang Wei W α6468 4.62% 96.08% Wang Wei W 0*2872 105 % 98.13% 直粉粉-0.09485 0.08254 -0.09687 0.69088 0.49756 -039747 0.18151 Total nitrogen content 038482 0.15967 -0.06458 -0.05595 -025752 0.09901 0.64242 Alpha acid or test protein 0.44969 -0.03858 0.06517 -0.09239 0.17608 0.13993 -0.19220 β· Acid or test soluble protein 0.45534 0.01382 -0.04203 0.07270 0.12353 -0.09667 -0.18652 Main alcohol soluble protein 0.45048 0.04747 0.09249 0.11733 0.00993 -0.13795 -0.02082 | Total retention protein 0.46892 0.01250 0.05317 0.05039 0.08477 -0.04670 -0.11729 'Partial viscosity -0.03504 - 0.16673 0.48161 -036981 037061 -022429 021689 The constant temperature strength point is 0.01556 -0.43506 028581 0.05155 0.08194 0.0 8019 037541 Number final viscosity -0.03634 0.45248 0.08569 -023367 0.14119 -0.15689 038790 Viscosity cracking value -0.05074 0.40671 0.06128 -039211 022286 -030010 -028649 Viscosity recovery value -0.00490 0.45303 -024786 0.06247 -0.13342 0.02633 0.15443 Grain length -0.05384 030720 0.39996 ( Χ21552 0.14067 0.49418 -0.06810 Grain width -0.00229 -0.02960 -0.43200 -0.17761 0.61401 054238 0.10460 Grain aspect ratio -0.04756 027550 0.48611 024446 -0.06560 026756 -0.09219 8th 9th 10th 11th 12th 13th 14th main component Main component Main component Main component Main component Main component Main component Characteristic root 0.1527 0.0671 0.0417 a〇OQ2 0.0001 aoo% Percent variation 〇»/«〇1.09% 0.48% 030% 0M% 0.00% aoo% 0.00% — Cumulative variation percentage 9922% 99.70% 100.00% 100.00% 100.00% 100.00% ιοαοο% 1 Direct powder -0.16608 -0.01841 -0.17620 -0.00305 0.003% 0.00000 0.00000 Total content 4*57039 -0.02748 -0.06440 0.00321 0.00656 0.00000 0.00000 α·Acid or alkali soluble protein 0.10844 0.05315 -0.78909 0.18908 -0.15164 0.00000 0 .00000. β·酸 or test soluble protein-0.05515 0.70050 0.41654 020005 -0.13697 0.00000 0.00000 Main alcohol soluble protein 023479 -0.64923 0.34221 0.30718 -025465 0.00000 0.00000 Total storage protein 0.13065 -0.08826 0.05508 -0.67291 0.52036 0.00000 0.00000 parts Peak viscosity - 0.05756 0.01851 0.05424 -0.00090 0.00125 036301 -0.47875 The constant temperature strength viscosity 024243 0.10686 0.03029 0.00099 -0.00396 0.15567 0.69044 The final viscosity 0.45685 0.15238 -0.02614 -0.00121 -0.00155 -0.55169 0.00000 Viscosity cracking value -035946 0.11971 0.00932 -0.00206 0.00615 0.12227 0*54230 Viscosity The rebound value is 0.38537 0.10403 -0.05517 -0.00034 -0.00200 0.72435 0.00000 Grain length -0.08973 0.00842 0.08943 -039243 -050749 0.00000 0.00000 Grain width -0.02033 -0.12406 0.18036 0.13684 0.17826 0.00000 0.00000 — _. Grain aspect ratio -0.05562 0.02224 0.01746 0.45201 0.57722 0.00000 0.00000 19 -°月> Reading Table 7' is a domain analysis analysis of the length and width traits and physical and chemical properties of rice grains of domestic and foreign Koshihikari rice in the examples of the present invention. Wherein the variation of the percentage of the first main ingredient of 31.66%, the cumulative percentage of variation of the fourth main ingredient to (iv) may%. According to the results of the main component analysis method, each main component can separate the two light whites of Japan, the United States, Australia, and Taiwan. Since the importance provided by the eye-catching sub-division is equivalent, the embodiment of the present invention incorporates the long-width traits of Koshihikari rice and the main components of physical and chemical properties into the discriminating method. • Discriminant analysis; Discriminant analysis (4) is a dependent method, the criterion variable is a pre-defined face or group to determine a set of variables in two or more previously defined continuations There is a difference between the number of flat sentences and the number of violent people on the 50th, and it is determined that the variables of these predictions can best change the difference of the average scores of two or more groups. In the embodiment of the present invention, the differential mouth knife and the production area are shown in Table 8. The results of the differential analysis using the rice grain length and width traits and the physical and chemical properties are shown in Table IX. Table 8: Sample number for differential analysis and its production area 曰本曰本Australia Australia United States United States United States 曰This sample number Kol Ko2 Κο4 Κο5 Κο6 Κο7 ΚοΙΟ Koll Origin Sakamoto Niigata Prefecture Sakamoto Niigata Prefecture Australia Australia USA California USA California Hualien Yilan sample number ΝΚ1 ΝΚ2 ΝΚ4 ΝΚ5 ΝΚ6 ΝΚ7 ΝΚ8 Sample No. W4 W5 W7 W8 W11 W14 W16 Origin Taitung Taitung Changhua Changhua Yilan Miaoli Changhua 1375795 Eyes See Table IX 'These are the invention __, the outer rice of the rice The wide traits are in the sub-division of the fine-grained area, and the _ _ _ _ _ ^ system can completely distinguish _, the outside of the light white rice production, and misunderstanding zero. Table IX, =, the difference between the length and width traits and the physical and chemical properties of rice

Data Code Australia (AU) Japan (JP) (% US (us) Discrimination Result

', total nitrogen content, alpha-acid or test soluble protein, p-acid or test soluble protein, alcohol soluble self-class, Qi Ji, lion fiber, indicating wrong zz, grain length, grain width and grain length The width ratio is shown in Table 10. According to the results of the present invention, in combination with the method for distinguishing the length and width of rice grains 21 2 "**,, 1375795 and physicochemical #, the domestic production area can be accurately distinguished. The origin of white rice is east or _west, and there is no error. The table shows the length and width traits and physical and chemical properties of rice grains in the east and west of Vietnam.

Distinction analysis result

2 “**,, • In summary, the method of using the appearance and physicochemical properties of Koshihikari white rice to establish a production area in addition to the above-mentioned example of the hair of the month, in addition to the fine transfer points _, Geng Yueguang from the rice outside 'To distinguish between the east and the west, the more Duomi, and its completely unmistakable judgment, the discriminating factors of the traits and physical and chemical properties are different, and the difference can be reduced to Zero. Therefore, the method of the embodiment of the present invention can achieve the purpose of accurate and rapid discrimination 'for the purpose of improving my __# management, and providing the consumer with a clear indication of the origin, thereby protecting the rights of the producer or the origin, and Maintenance Consumer 22 1375795 [Simplified Description]

The first figure is a flow chart of a method for discriminating a Koshihikari rice production area according to an embodiment of the present invention. The first factory picture is the length of the record and the width of the rice grain.

The third figure is a protein electrophoresis pattern of a part of the Koshi white rice sample of the embodiment of the present invention. No.: protein molecular weight marker (marker); _^ Yu: Taishou No. 9 (τκ9); third line: Yuguang white rice (W14) from Miaoli; fourth line: produced by Changhua Five elements: another - produced by Changhua's Yueguang white _); sixth line: production ^ T's Yueguang white rice _ 〇) '·The seventh line: from Vietnam's Yueguang Bai Miqing); the first production from the United States The ninth line of light white rice: the more white rice produced in Australia

[Main component symbol description] 1 Koshihikari rice grain 10 m grain bottom 12 m grain back L1 rice grain length W1 rice grain width 11 m grain tip 13 m grain abdomen S10 S20 S30 S40 S50 S60 Collection of known production area Koshihikari rice sample measurement rice grain length and width and physical and chemical The nature of the chemical analysis and establishment of the Kuangwumi white rice production area discriminating model to measure the length and width of the sample and the physical and chemical properties of the test ^ with the Kwangwon white rice production area difference between the test sample of the origin of the copper sample to be tested abroad , domestic eastern or domestic western 23

Claims (1)

U75795 X. Patent application scope: 1 The appearance and physical and chemical properties of the use of Koshihikari white rice include: (1) Measuring the length of the Koshihikari, the width of the rice grain and the physical and chemical properties of the production area, and statistically analyzing the measured values Establishing a production zone discrimination mode including the green length, rice grain width and physical and chemical properties of the known light-emitting white rice; (2) measuring the length of the rice grain, the width of the rice grain and the physical and chemical properties of the Koshihikari white rice and inputting the measured values into the production zone discrimination mode of the step (1); and (3) the statistical analysis and the comparison step (2) The values to be measured by Koshihikari and the values of the steps (1) of the known production areas of Koshihikari rice, thereby determining the production area of the Koshihikari rice to be tested; The method of determining the nature of the production area, wherein the physical and chemical properties are selected from the group consisting of amyl〇se content, total nitrogen content, and storage protein (such as ^ρΓ〇_ containing $ and viscosity ( The group consisting of the viscosity. 2. If the method described in the above paragraph is applied, the length of the towel is the longest distance from the tip of the rice of Koshihikari rice to the bottom of the rice grain. The method according to the first aspect, wherein the width of the towel I is the longest distance from the back of the rice grain of the Koshihikari rice to the abdomen of the rice grain. '' 4. The method of claim i, wherein the storage property The protein content is an acid or alkali glutelin content, a prolamin content, or a total storage protein obtained by summing the acid or alkali soluble protein content and the alcohol soluble protein content (total storage) The method of claim 4, wherein the acid or test protein is an alpha-glutelin or a beta-acid or a test protein ( Β6. The method of claim 1, wherein the viscosity is selected from the group consisting of a peak viscosity, a holding strength viscosity, a breakdown viscosity, and finally The viscosity is full of viscosity and the group consisting of the setback viscosity. 7. The method of claim 1, wherein the statistical analysis is Principal Component Analysis. 8. The method of claim 1, wherein the statistical analysis is Discriminant Analysis. 9. The method of claim 2, wherein the production area is Taiwan or Countries outside Taiwan. 10. The method of claim 9, wherein the Taiwanese production area is in the eastern part of Taiwan or the western part of Taiwan. u. The method of claim 9, wherein the country other than Taiwan is the United States, Australia or Japan. 25