TWI356167B - Signal detecting method for biochip - Google Patents

Description

1356167

4 I. Description of the Invention: [Technical Field] The present invention relates to a method for detecting a missing A number of a biochip, in particular, a signal in a wafer that simultaneously obtains a biological component signal without performing component separation. Detection method. [Prior Art] In recent years, due to the rapid development of biomedical detection technology, analysis technology. Traditionally, this electrophoresis analysis has the disadvantages of slower speed and low efficiency, and it has the disadvantage of having a high inspection cost because it has to make a large sample amount.乂长和:颂f job 3 electrophoresis analysis method, such as #射引化=Ced fl_S_e) 'When performing this analysis, the profit = light dye sample irradiation excitation' makes the f-light dye sample 4 splicer, using light The detector detects the sample of the camping light dye to complete the fluorescence detection. However, the laser-induced t-light method relies on large-scale optical debt measurement systems, such as microscopes, and the structure of the heart and the heart::: learning, in general, large optical detection systems Has a body -,: very large. Disadvantages. Therefore, although the wafer has been miniaturized, it is difficult to apply a large-scale detection system.彳 疋 疋 still faces it only. However, if the wafer electrophoresis system is to be integrated into the wafer, it may be possible to achieve further steps to integrate the inspection and inspection mechanism on the wafer. The jujube can be large _ small scientific testing department 1356167 •, the volume of the system, and the detection results similar to the large-scale detection system, the material has a record, such as silk inspection _ structure must be another light source and light sensory equipment Although the semiconductor manufacturing technology can make the light source or the light sensor device into a small module, it still needs to face the problem of system integration. _ difficult, and inevitably faces the problem of requiring light integration. Re-utilizing the biochip detection method, the two molecular probes are respectively attached to the -conductive beads and fixed in the gap between the two electrodes, and the potential difference is applied to the electrode by observing the current increment of the electrode; The specificity of the reaction between the probe and a target to be tested is used as a basis for detection, whereby the determination of the target molecule to be tested is performed. (4) 'The above-mentioned method, whether it is optical or electrical inspection, still has many shortcomings. Among them, the big disadvantage is that the conventional technology cannot detect multiple components at the same time. For example: if the target to be tested When several different components are used, 'these methods must separate the individual components by a component separation method (such as dilution with a buffer solution) to prepare the target to be tested for purification. Then, the individual components are subjected to the above-mentioned conventional detection, so that the detection complexity is increased, and the detection efficiency is lowered, and the detection accuracy is insufficient. In view of the above, the present invention improves the above-mentioned disadvantages, by detecting a plurality of units in a biochip, and detecting a liquid crystal containing a plurality of different secrets, and by the signal analysis method of the present invention, The signals of the components are separated, thereby simultaneously obtaining signals of different components, and the detection efficiency and the detection accuracy can be surely improved. —6 — 行乡鲜, to (4)- 聪聪阵u, the estimated original liquid matrix s of one of the measuring liquids®2, in order to take the biocrystals [S1] of the preferred embodiment of the two embodiments Matrix X is waiting; car: and - mixing matrix one, the test is to be ===

In the step of X = AS (1) ^ [ s 2], the centering process is to obtain an average moment for the signal processing of each independent component:: tiger ^ establishes a two-mean matrix m, so that the signal matrix X of the signal to be tested is not numbered The moment "the value is zero; the whitening treatment removes the waiting

The signal of the Μ component is _ correlation, and the extraction process from the W q independent component is in the [S3] step, and when it is formed, the signal matrix x to be measured is reduced, and this '+interval_ The speed of the analysis of the secret analysis and complex (four). In the = ' right 彳 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ In the row dimension, f passes:, and the M column dimension is reduced to 1^ column dimension, and then the operation is performed. 1 夕二二^2图*, on the date of her hair, the biochip, the method of the second method [S4] is searched for - the target matrix function w is based on w, and the basis of Wei is based on the root T The matrix function w Μ is the inverse matrix of the hybrid matrix A. Referring to FIG. 2, in the present embodiment, the [S5] step of the signal detection method of the slice will be processed via the front. No. X and TM function milk truck transport #, in order to obtain the estimation matrix u,

u = WX 4 (wherein the estimated matrix u has the following relationship u%s (4) with the original matrix s ' With the above steps of the present invention, an efficient detection analysis can be performed for the body 2 of the organism. Referring to Figures 3 to 5, it discloses a waveform diagram of the signal detecting method of the above-described preferred biochip. For example, 帛3(8) to 信图7F 'the wire* is to be measured by two ray components The original to 4U Sl~s3 'the original' initial signal sl~s3 is an -unknown signal; as shown in Fig. 4(a)(c), it indicates the liquid to be tested 2 three sensing units different cats The signal xl~x3 of the three components measured under the positional condition (as shown in Fig. 1). As shown in the fifth (thick 5 (4) diagram, the operation can be performed by the independent component analysis method to generate - The group estimated signal is passed through the unknown original signal sl~s3, ' ". The U1~U3 signal ul has the same characteristics as the first shield, and the original signal of the figure 5(a); S3, that is, the estimated nickname is the same as the estimated signal of the 3: (8) graph, coffee: t (4) and 5 (the waveform and frequency of her and the original signal Sl) ::::Up:::Signal U3_ Each estimated signal ul~u3 and 3__== 5th si~S3 Although there is a difference in amplitude, ^4 is processed to make -Eul~ U3° hunting is the same as the amplitude of the normalization operation. The old and corresponding original signals sI~s3 ^ Tsjf The number of the calling unit 3 is selected to be larger than the number of components to be scraped (9) = or equal to the liquid to be tested 2 = 吸 2, right under this condition, correct the above-mentioned unique domain component to the rider 2 U components to generate N estimates · winter can be 'to this for the body of the body 2 Subdivided ^ ^ _ Assume that the number of sensing units 3 is less than the number of parts, when the number of parts is iron, and that the product is owed to the main two: L, or for the liquid to be tested 2 The W仃 analysis 'can generate the main part of the estimated signal and then' if the liquid to be tested 2 has an interference signal, secret

The above steps of the invention are carried out to separate the interference signal Z so that the liquid to be tested can be more accurately detected. In addition, the sensing unit 3 of the present invention is not limited to use only as a measuring electrical signal. In fact, the sensing unit 3 can select according to the physical quantity to be measured, such as an electric signal, a magnetic signal or an optical signal. Measure. As described above, compared with the conventional biochip detection method, the analysis of several components cannot be performed at the same time, and the detection complexity is increased and the detection efficiency is low. The signal detection method of the biochip according to the embodiment of the present invention is for the liquid to be tested. 2 The money corresponding to several mixed components contained in the analysis is simultaneously analyzed, which can indeed provide (four) surface analysis, improve the efficiency of the extraction process and reduce the detection cost. Although the present invention has been disclosed by the above preferred embodiment, the heat is not used. The invention is not limited to the spirit and the scope of the present invention, and various modifications and modifications are made to the above-described embodiments. The scope of protection of the present invention is regarded as the scope of patent application. The definition is final. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic diagram of a method for detecting a biochip according to a preferred embodiment of the present invention. Fig. 2 is a flow chart showing the steps of a signal detecting method for a biochip according to a preferred embodiment of the present invention. / Fig. 3 is a schematic diagram of the signal of the biochip according to the preferred embodiment of the present invention. The original signals S1 to S3 of the hypothetical liquid to be tested are shown.

Fig. 4 is a diagram showing the signal detection method of the biochip according to the preferred embodiment of the present invention. The waveform diagram of the ##1 to x3 of the three independent components measured by the two sensing units under different positional conditions is used.

Fig. 5 is a schematic view showing the waveform of the estimated k number u 1 to u3 generated by the signal detecting method of the biochip according to the preferred embodiment of the present invention. [Description of main component symbols] 1 Biochip 11 Inlet tank 12 Outlet tank 13 Channel 2 Liquid to be tested 3 Sensing unit U Estimation matrix X Signal matrix to be tested S Original signal matrix W Objective function matrix m Mean matrix xl ~xN independent component signal sl~S3 original signal ul~uN estimated signal—13 —

Claims (1)

Patent application scope: The signal detection method of the biological chip uses several sensing units for 3, sudden and somatosensory, and one of several different components to be tested is not taken out to extract the signal matrix to be tested; The matrix of the signal to be tested is subjected to an average value of the signal barrier of the signal to be tested in the front tube: one: = ^ removes the correlation of the signal transition of the phase; = the matrix of the signal to be tested is degraded after the step of processing the difference Selecting a private function matrix, and computing the objective function matrix to receive; and performing a matrix operation on the signal matrix to be tested and the convergence objective function matrix after the processing operation and the reduced order operation to obtain an identical Or approximate to the estimation matrix of the original signal matrix. 2. The method for detecting a biochip according to claim 1, wherein the original signal matrix has a plurality of independent original signals, and each of the original signals is a signal of a different component of the liquid to be tested. 3. A method for detecting a biochip according to claim 1 or 2 wherein the matrix of the signal to be tested has a plurality of independent components to be tested. The words of each individual component are distributed as non-Nuss. 4. The method of detecting a biochip according to claim 1 or 2, wherein the number of the sensing units is greater than the number of components of the liquid to be tested. 5. The signal detection of the biochip according to the first or second item of the patent application scope 5=the number of the unit_the number of the unit and the number of the component to be serviced, and the creatures mentioned in the first or second patent range The number of units of the crystalline signal detection Θ, /, towel is less than the number of components of the standby body. A method for detecting a biochip according to claim 6 wherein the estimation matrix separates signals of the main components of the plurality of different components. The method for detecting a biochip according to claim 1 or 2, wherein the signal matrix to be tested further comprises a mixing matrix, and the mixing matrix performs matrix operation on the original signal matrix to form the signal to be tested. matrix. 9. The method of detecting a biochip according to claim 10, wherein the target matrix function and the mixing matrix are inverse to each other. 10. The method for detecting a biochip according to the invention of claim 2 or 2, wherein the biochip is provided with a liquid inlet tank, and the sensing unit is disposed adjacent to the inlet tank of the liquid to be tested.