TWI338695B - Plant tubby-like protein gene family - Google Patents

Description

</ RTI> </ RTI> </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> </ RTI> Into this article. TECHNICAL FIELD OF THE INVENTION The present invention relates to plant dwarf gene proteins and 4 isolated nucleic acids encoding the proteins. [Prior Art] Many different environmental factors, such as high salinity, pathogens or low temperatures, can exert stress and negative effects on crop growth and yield. Therefore, it is necessary to produce a genetically modified crop that can tolerate such factors or to use genetic engineering techniques to modify proteins involved in the regulatory response of plants to environmental factors, thereby improving pressure resistance. The chunky gene (TUBBY) protein is a membrane-bound transcriptional regulator that was first identified by positional selection in obese mice (Kleyn et al., 1996, Cell 85: 281_29〇 and N〇ben_Trauth et al. ' _ 1996' Nature 380: 534-538.). Mutations in the chunky gene (TUBBY) result in obesity, insulin resistance, retinal degeneration and loss of neurosensory hearing after maturity. Later, the dwarf gene proteins (TULPs) were also found in other mammals and found to be activated by g·protein.

SUMMARY OF THE INVENTION V

The basis of the present invention is to find 11 kinds of dwarf gene proteins, such as AtTLP 1 -11, which are involved in the regulation of Arabidopsis thaliana to environmental factors. AtTLP1-n polypeptide (referred to as SEq ID NOs: 1-ll) and cDNAs encoding the polypeptide (referred to as SEQ ID N〇s: 12-22) such as 91261.doc

AtTLPl : Peptide:

[MSFRSIVRDV RDSIGSLSRR SFDFKLSaH KBCXi KSRGSV QDSHEEQLW 51 TIQBIPWANL PP0-LRDVIK RLEESESVWP ARRHWACAS MCRSWRDMCK 101 ElVQSPELSG KITFPVSLKQ PGPRDA.TMQC FIKRDKSNLT YHLYIjCLSPA 151 aVENGKFLL SAKRIRRm TEYVISW1AD TISRSSmi GKIRSJFIjCT 201 KFHYOT ^ A YNSNIARAVQ PVGLSRR FYS KRVSPKVPSG SYKIAQVSYE 25 I LNVLjGTRGPR RMHCAWMSIP ASSLAEOGTIV payDl IVPR SILDESFRS1 301 TSSSSRKITY DYSNDFSSAR FSDILCPLSE DOEWLEECK ERNSPPLVLK 351 NKPPRWHBQL QCWCLNFRGR VTVASVKNFQ LIAANQPC3PQ PQPQPQPQPL 401 TQFQPSOCirD GPDKIILQFG KVCBCDMFTKO FRYPLSAFQA FAICUSSFDT 451 KLACE (SBQ [D NO: 1&gt; cDNA :

ATCrPOGTTOC ATOCAGOCXrr OTAAATODCB ACX3ATTCAAG TCTGATCAAA ATGnOTTOC GAGATTCHTC GTTGAAACAG GOGATAAATC TTGTTOCnTQ AACTACATAC GATCAAOCAA AACTITATAA AGCGGPOCAA CTOXAAAGT ctaaaogttc CTCAATTOOC COOATATCAT ACCTCTTCCT CXGTOCAOOG TGCTTATTAGA AACAAOOOOC: CAGGCSGACCT CAAAOCAACE 51 101 151 201 251 301 351 401 451 501 551 601 651 701 75 L 801 851 90] 951 1001 10S1 1101 1151 1201 1251 1301 1351

GTAOCATAGT TOCTGATGTC AGAGATAGTA TAOGAAC?TCT

AGTTTOGACr TTAAGTTAAG CAGCTTCAAC AAAGAAOGTC IlXTnCGC/lT CAAGATTCTC ATGAOGA ACA ACTTUTAGTA OOOCiAATCTA CXTOCAGACTT TATTACGTIGA AGAGIGAAAG TUTCTIOOOCT OCTOCTACAC nurocTicr GrrrocAOGT catogagaga tatctCjTaaa

AAACmOCXXA GCTCTCAOCX: AAAATCACAT TTOCIGTTTC GAGATOCAAC AATOCAATOC TTTATCAAAA TAACITCACT TATCATTTAT ATCTTrcTCT CAGTXXTOCr ACAATQGAAA OTTTCTTCTT TCTOCAAAAC OCATAAGAAiG ACEGAOTACG TO ATCTCTAT OCAGOCDGAC AOCAi 1TOGA TACCTACATr OGCAAAATCA OCnCTAATTT TCTOOOGAOO TATAOGATAC ACAACCAOCA TACAACAOCA ACATG0CIO3 OOOGTAOCTC TTM3CCOCAG ATTCTACPCA AAGAGAGTCT AOCTAGTDOG AGCTACAAAA TTOOGCAGGT T1C1 1ATGAG TTOGTAOOCG TT3GT0DGAGG AGAATOCATT GIOCGATCAA TTCXDGGAAOG GCXjAACTCTPCJ OCTOGACAOC TCTATTCTGG AOGAATOGTT GCXX ^ VGCATT CATOGAGAAA AATCACTTAC GATTACT03A ATGAmTAG TITTGOGACA TTOTOOOOC GTTAAOOGAA GACCAAGAAG AGAAGOGAAA GAOOOGAATT ODGCAOCACT TCTCCTTAAG GGAGCTTGOCA TCAACAOCTT CAGKJTTOGT GrTTTAAACIT CTTAACACrrCIf CATCAGTTAA GAACTTTCAG CTCATTOCAG

&lt;XAGTTTO〇G OOCTCTCTOC AAACTTOCrT

AACTOCAAOC TCAAOXCTA TCAGAC03AT OGTOGOGACA AGATCATATT AAGACATCTIT CAOCAlXTAT TTOOCSGTATC TTOOCTATCT GI I UaAGCAG TTTOGACACA (SEQ ID NO: 12) 9126l.doc 1338695

AtTLP2 : Peptide 丨51101151201251301351

MSLKSiLRDL IPPQ1HD1I

KEVRDGLOGI SKROTCSSH Shu APDQTTPPL DN1PQSPWAS WRVEESETAW PARMWSa SVCKSWRCIT MEIVRIP BQC GKUITISU QPGPRDSP1Q ffiKRNRATA niLYYCIMP SEIBNDGiX AARRIRRATC 1DFIISLSAK NFSRSSSTYV GKLR9GFIGT KFTIVDNQfTA SSTAQW3FNR RLHPKQAAPK LPTNSSTVGN ITOmfiT fiGPRRMHGW DSIPLSSVIA EPSWQOIEE EVSSSPSPKC ΕΓΠΜΕΙΡ DNSPSLRDQP LVIXNKSPRW HBQLQCTON FKCRVIVASV KNPQLVAEID ASLDAPPffiH GKDIFIWDYR

ERVILQRKI YPISAfWA ICISSFErnCP ACBG (SBQ ID NO: 2) cDNA :

(JOGATOGACT 1 1 tl 111 —4 1 l —i 1 14 1i III II 11^ H 5IOI520253035404550S5606570758085909500051015 ATCTCnTGA AAAOATOCr TOlTOATUItj

TOGAOOCATC TOCAAGAGAA GCTOGTCAAA GTOGTOCAC ATTGOOCrG ATCAAACAAC TOCAOCACTC GATAACATAC CACftGAGOOC ATOOGOTCT TTCOOGGCTC ACITOCTrCA TCAamTC TOGAOOOTC AAGAGACKIA GAOOcrroc oooxtogag ααπτπχττ crcrrcrocr tcacttatcta

AATCATOGAG AGGAATCACT ATOGAGATIG TGAOGATOOC TGAOCACTCT OOOAAOCTCA CimCCAAT CTCATTGAM CAODOTOOC CTOGAGAOC TOCAATTCAA ΤυΓΓΓΓΑ ™ AGAGGAACAG AOCAACAOCT ACATAaiTC ταΑπΑτασ τγκιατοοο · toogagactg agaaocacaa αοκτγκπτα OCAGCAAGAA OGATTAGAAG AOOGOTOC ACACAOTTA TMTCPOCCT ATCTOCAAAG AACTOCAC C3GAi3a〇CAG TACTTATCHT OOCAMHAA OOTCIGGrTIT TCTGOGAAOC AAOTTCACAA TATATCACAA OCAAACAOCA TCATOCACAG OCAAOOOCA AjOCTAAOOGA AGAC1OCAOC OGAAACAAGC GOCTOCTAAA CTAOCTACGA ATAXTUTAC OCTAOGAAAC ATAAOCTAOj AOcrcAATCrr icrnoocAa agoggaccta gaagaatoca ciooxtatg GATTCTATAC OOCIUIUITC TOTATIOCr GAACaTTCAG TACTTCAAOG OTACAAGAG GAACICTCIT OCICTOCTTC AOCAAAAC3GA GAAAOCATCA CAACAGACAA AGAGATICCT GATAATOC CAAGCITAAG OGAOCAAOCXJ CTAimna AAAACAAATC OOCAAGATOG OVTCAGCACTT TOCACHtOG CTGOCTCAAC TTCAAOOOAA GACTTGACnTT GOCnCACIT AACAATTia:

Aocrrcrroc agagattgac αατκπττοσ Ατοοοαχχττΰ aagaacat GAGAOOCiroV TCITACAffrr TOOCAAAATC OGTAAGCATA TmUOCAT OGAHATOGC ΤΑ(30ΠυΓΑΤ CTOCTlTra AGCUTTOCT ATATOCATO OCAOCTITGA CAOCAAAOOO OCATtTTOAAG OG (SBQ ID NO: 13) 91261.doc 1338695

AtTLP3 : 吝 peptide:

1 MSFKSUQDM RGELGSISRK (FDVRPGVGR SRSQRWQUT SVPVDAFKQS 51 OWSMimL RDVUKRmjS EDITOKNV VSCAGVC pulse REIVKEIVRV 101 PELmiFP ISIKQPOTG SLVQCYIMRN RSNQTmYL ONQAASNDD 151 GKFUMRF RRPIODYII SLNCEDVSRG SKmGKLRS NFUnXFW 201 DAQPINPGilQ VTRTTRSm SLK_ [P SGNYPVAHIS YaMGSRG 251 PRJM3CVMDA IPASAVEPOG TAPHJIiLVH SNLDSFPSFS FFRSKSIRAE 301 SLPSCPSSM QKEGLLVUN KAiWHJUQ CCUTORV TVASVKNFQL hearing VAAPENGPAG PEHENmQF GOT )· DYQiPISAFQ AFnO^SFD 401 TXIACE (SBQ (D NO: 3) eDNA :

1 AlUTOOra AGAGOOT TCAGGACATG AGAOGAGAOC TTOAtJTAT 51 ATOCAGMAG OGATOMG TCAGATTCOG CTATOGTAG ^ manat:! 101 ManUnumOGATACT TCimTOCTC TTOTOCnT OAOCAGAOC 15 TCCTO00CTA CTATOOCTOC GGAflOOCIG ship TCITC ITATCAOGAT 201 TCAOCAATOC GAAGAGCIT GOamAG GAMMTGTT CrnUITO 251 CraGKHCTO CAOGAACTOG 0GAGAAAT0G TCAAAGAGAT anCACAOTT 301 COGAGCTIT CTAflCAMCT CACnrorr ATOOOOCA AACAGOOOOO 351 I0CTAGA0GA 1CAOTTTC AATOCTATAT MMGAAAC OGCAOCAATC 401 MAOCTACTA TCTATACCTC amAAAOC AAOCAGOTC AMTGATGAT 451 ΟόΑΑΑίΤΓΠΧ: TTOTODC (ΜΜΠΤΓ OGGAGOOCAA CITGOOGA 501 CTAQUaiC TOOTMACT OOGATOTCT CICTOGAOGA AOCAATAOT 551 ' ATATOGAM QCITAQAICT AACITOQG OGAOCAAOT CACimCTAT 601 GACGCTCAOC OCA0GAAT0C TOAAOCAG CTTAOCAGAA CODOTCAAG 91261.doc 1338695

651 CAGCnOC AGHTGAMC AAGIGAGGOC GAGAATPOCA 1CTO0CAACT 701 ATOOUTAOC ACATAim TATCAOOTA AanCTTOGG TO〇a〇GA 751 CCGAOGAOGA TGCAGTGTGT araATQOC ATOCFOCAT CAOCTCTAGA 801 AOCTOAOGA OOCTOCM OCAGAOGGA AOTUTOCAT AOCAAOTC 851 ATAGrnar Cianuia: nunaOGT OGMATCAAT TanOCAGAG 901 Αοταοητ crocmcATc aiogoot cagwogm 〇gocctot 95i OCTCMMAC MAGOOOOCA GATOCACGA ACACOOCAG TCCnMJOC 1001 TCMCITCAA TOOGAGACTC ACACJmnT OCmOAMA OTFCAOOTG 105i CTAGCKOC CTGAGAATOG AOCTOCAGGA ccnmoc ΜΑΑΟΠϋΑΤ U01 TCTOCAGrrr OGAAMGTOG GAMAGATGT GTICACAATC MTATCACn: U51 ACCrrATOC TO0C1TCAG amOOCA Trramo CAGnTOOAC 1201 AOCAAGATAG CATOTGM (SBQIDN0:14)

AtTLP4: polypeptide: 1 MPPEUm MRtERSHDW PSRKNWSCV GVCMQ1F KEIVNVPEVS 51 SKFIFPISLK QPGPOGSLVQ OVK8NR3Q TFYtmCEA KIFOQSBPSD 101 1YLVPYSYRE THCVMDA1SA SAVKPGCTAT TtJIHJWFVS FRSPSGQKffi 151 VLVUCSTO LEHJSKUJF NGSPENEPEN measured RjFAKV GMiKLFSLY 201 EAEWLVRT SVFAV [ARVC RO0OTPSYE LKULYFAKN SA1LKKFVLR 251 GVTOEDLLALPVAN (SBQIDN0: 4) 91261.doc • 10- 1338695 cDNA :

I ATGOOTTIG AOGTCIGAG AOMOTCTC ATGAOGATAG AGOGATOGGA 51 AGACACTTOG CCnnm AGMOTCT ΤΙΓΓΚΠϋΓΑ αΠϋΚΓΚΓΓΑ KU AGMCTOOOG ACMATATTC AMGAGATCG πΑΑΟΠΤα: TCAGGTnCT 151 AOCAAATTCA cmroc AAT CroaTCAAA CAOOCroGTC OOGAOGATC 201 ACTIUnOA TOCTATCITA AGAGAAAOOC TAOCAATCAA ACmCTATC 251 TATAOCTTOG AOGTCAAOCA ΑΜΑΤΑΤΠΤ (ΠΟσίΠϋΑ AOCAAffTCAT 301 AnTATCTOG TTOOTACAG HACAGAGAG AGOCATITO TCATOGATOC &gt; 351 CATCICTXA TCAOCAGTAA AAOCTOGAGG AACAOCTACA ACTCAGACAG 401 AACT03ATAA UTOTOTCA TTCAGGTCIC CTICTOCTCA MAOGMOGA, 451 GTOCntHTC UAAGAOCAA AGKXCTAGA TTOGAAGAAC AGAOCIOTG 501 TOT3AC7K: MTOOCTCIC CTOAGAAOGA AGCTCAGAAT GAAAAOGACA 551 TTITOCAfflT TDOGAAAdlt: ΟΟΑΑΑΠΤΧ ACAWOCIT CACTTITATAT # 601 GAGGCTGAAT OGAITOCTCT 0GTT00CA0C TCAGTCrrrc CKJrCATTOC 051 TOGAOTTOT AGAGATAAM AOCATACACC ATOTATGAA TTCAMCTIG 701 OTIUTAOT TOCAMAAAC TCTOCMTOC TCAAGAMH ΟΠΤΟ TOOOC 751 (ΧΤΓΓΑΟΟ: GAGAAGAAGA mOOXA TKmOTG CTAAC (SEQ ID NO: 15)

AtTLP5: polypeptide: 1 MSFLSIVRDV ROTOSFSRR SFDVRVSNGT THJRSKSHGV EAHIEDLIVI 51 _WANLPA ALLRDVMKKL DESESIWAR KQWACAGVC KmMCKDl 101 VKSPEFSGKL TFPVSLK (yG PRDGUQCn KRDKSfWnrH LYLSLSPAIL 151 VESGKFLLSA KRSRRATYIB YVISMDADNi SRSSSTYICK LKSNFUnKF · 201 IVYDTAPAYN SSQILSPPNR SRSFNSKICVS PKVPSGSYNI AQWELMX 251 (TITiCPRRMNC IMHSIPSUL HPOGTVPSQP EFLQRSLDES FRS1GSSKIV m KHSGDFrePK EHEC1CVRPLV UOKPPRWU} PLRCWCLNFK CRVTVASVKN 351 FQLMSAATM3 PGSGSDOGAL ATT^PSLSPQQ PBQSNKDK1 i LHFGICVaCDM , 91261.doc 1338695

401 FTODYRYPLS AFQAFAISLS (SEQ ID NO: 5) cDNA: 51 o 5 Ό 5 112 2 11 nnn 0 5 0 5 050505Q505015051051 7&amp;8^9woll^2 14 11 I* 1 tM —i ΑπΠΠΓΓΓΚ: TGACTATIGT TOGTCATCnT AGAGATACIG TAGGAAOCTT trOGAGACCT AGnTOGAOG TCAGACTATC TAATOOGAOJ ACTCATCAGA OGAOTAAATC TCAOKnUTT GAC50CACATA TTGAAGATCT TATIUTMTC AAGAACACTC (ΠΤΟΟΟΟΓΜ ΙΓΓΑΟΟΟΟΟ * OOOCTAmC GAGATCTCAT GAAAAACmG GATGAAAOOG AGAdTAOTG OOCTOCAOGT AAACAAGKX; ΤΠΧΤΙϋΓΧ TOOTCTCroC AAGACA1GGA GACTMTCTC CAAAGATATT GPGAAAAGTC CTCAGTICTC AGOCAAACTC ACATITOCAC ΤΓΚΧΠΤΟΜ ACAOOOOOGG OCTAOQCATG GAATCATAa AKHTATATC AAAAGAGO ACTCTAACAT GACITAOCAC CnTAOCTIT CTOTACTC C TOCTATACIT OTJAAACTG OGAAOmCT TCTCTO »C \ AAOOOCTCAC GGAGAGCTAC ATACACAGAG TATCTAATAT CAATOGWOC AGACAACAn TCAAGATCAA CAT CATTOOCAAA CIGAACICTA ACrTICTAGG GACAAAATiT ATAOOCCnX TOOCTACA AC AGTAOOCAGA ΤΑΤΚΠΤΠΤ ACTOCTACTT TCAACTCCAA GAAAGICTCT OXAAMJItX TTACAAan OCTCAAGITA aTAOGACJCT GAACTTOOT OGAAOCDCTG CADCiaTTAG AATGAACTOC AHATXAQ ΟΆΤσΧΓ Π: CITACOCTA CAAOXCCAG GTACTCrCDC TAOX ^ AOCT ManrooCT tcatcaatct ttooxaoca TCDGrracn: AAOCACIOJG GAGATITCAC OOGACCGAM GAOGAAGAAG GAAAOGTOGC AOdTIOCTA CTCAAAACTA AAOCOCAAG GrOOCTOCAA OOCrTTOOGAT OTTOCTGCrr TAAOTOAA OOGAGAGTCA CTOTAOCnC TCTCMM: ITOCAGriGA TGTOCX3CTOC AACOOTCAC OOOOCTACTro GTACICATOC TOGACJCATTO OCTAGGAOX CAKXnTATC AOCACAGCAG OCAGAOCAAT CAAAOCATCA TAAGATAATA CTACACnTC OGAAACITO TAAOGATATC TOCTATQG ACTATOCnTA TOnCICTCT OOCmOAG aJTTTOOCM rranGAGC AOCmGATA CTAMTIOOC ATGTGAA (SBQ ID NO: 16)

OCACJCAOTA ATACTATATC

* GAJnTCTAC AAAGATACHC

AtTLP6: polypeptide: 1 HPLSRSLLSR RISOTFHQ ΟΕΤΓΓΑΡΕ ^ SIPPPSNMAG SSSWSAMLPE 51 LLGE1IRRVE EIOTPQRR DWIOCVSK with EITUDFA RSSLNSGKIT 101 FPSOJdPGP RDFSNQCLIK RNKKTS1FYL VLOPSFTO KQCFLUARR 15i FRTGAHEYI 1SLDADDFSQ GSNAmR SDFUHNFIV YDSQPPtta 201 KPSNGKASRR FASKQ State PAGNFEVGHV SYKFNUKSR GPRRMVSHR 251 CFSPSPSSSS AOSSDQfTC surface _ KDGSSLTIUC NKAPRWBi 301 QCXWHGR WASVKNPQ LVmxjSQP SGKGD6ETVL LOFCKVOTT 351 TOYW AFWAiaT SPGTXUCE (SEQ1DN0:7)

9126l.doc 1338695

cDNA

1 ATOTCATTGA AGAACATAGT GAAGAACAAA TACAAAC3CTA TTOCTrAGAAC ACXjGAGCTTCA CACATTOCAC CAGAAOGMC Aintnxjrcr TCTimTAT 101 CAACTAATGA AOGTTTAAAC CAGAOTATTr ΟσσΠϋΑΓΓΓ GOCTOCAGAG 151 HACITCmG ATATAATOCA AAOGATTGAG TCKJAACAGA GTTTATCGCC 201 OGOGAOGAGA GATCTTCTTG CriCTOCTK: amTGTAAG TCATGGAOGG 251 ACATGACTM AGAACrTGTT AAAGTTOCTC AOCIOCTOG TTTCATCAaJ 301 THUOGATIT CTITAAGAa OdnOGAOCT AGAGATGCIC CAATICAATG 351 αΤΤΑΓΓΑΜ OnUAAAGAG CTAOOQOGAT ΑΤΛΟαΤΟ: TATCTTOOT 401 TAAOCCCIOC TCnTOGOGT GACMGAGTA AOTIUTIUIT ATOQCAAA C 451 501 551 601 651 701 751 801 851 901 951 1001 1051 1101 1151

AGACTTCAOGA GACJCX3AC00G TOGOGACTIT GTIUTATOGT TATOCXXXiAA TGACITCTOG AGAAGTAGTA GTAATOCAT AGGMAACITJ AGATCAAATT TOCTOOGAAC GAAGTTCACA GTCTAOGAAA AOCAAOCTOC TOOGTTTAAC OGAAAGCTGC CAOCATOGAT GCAACnUTCT OCATOOCT AT OGTOGTCATC TACrTAGTTAC AACATACXTT CAATCTKTTA TCAGCTGAAT GTIOGAGAA (XAGAGGTTOC AAGAAGAATG CAATGTATAA TGCACAGTAT (XOGATITCA OOGATTCAAG AAGOGOGCAA AATOCAGTOG OCAAGOGACTT TCACAAA (DCA AOGAMGAAG AAGAAGAAOC OOCTGAT CX1A mUTOCTCA GOGAAOCTOG GAOGAGAATC ΟΠΤΑΤΑΑΑΑ GAAOCATTAA ΤΓΟΌΑΑΑΑΑ CAAGTOC300G AGATOOCACXJ AACAGCTTCA GTOCI0G1OT CTAMCTTCA AAOCTTOGAGT CACAGTOOOC TOQGTGAAAA ACTTOCAOCT AGTOOCAGCT COOCAGAAG CAOjGAAGAA CATCAACATA CCAGAAGAOG AACAAGATAG AGHjATATTA CAGITIOOGA AGATAGOCAA AGACATTITC ACAATOGATT ΑΤΟΠΤΑΟΧ GATCTOC3CA TTOCAAGCrr TTOCTATITG TITAAOCAGC TTOGACAOGA OGAA

AGOCAGTOG (SEQ ID NO: 17)

AtTLP7: Peptide:

MPLSRSLLSR RISNSFRFHQ GETITAPESE SIPPPSNMAG SSSWSAMLPE 51 LLGEIIRFVE EITEDRWPQRR DWIOCVSK KWREiTHDFA RSSLNSGKIT iOl FPSOJCLPCP TOQCLIK RNKKTSTFYL mTPSFTT) KGKFLUARR FRTCAYTEVI 1SU3ADDFSQ GSNAYVGfOR SDFIJGTOFIV YDSQPPHNGA 151201 251 301 351 KPSNGKASRR FASKQISPQV PAGNFBVGHV SYKFNUXSR GPRRMVS1LR CPSPSPSSSS AGLSSDqKPC lynCIMKKPN KDGSSLTILK NKAPRWHEHL (PCWHGR WASVKNPQ LVATVDQSQP SGKGDeETVL l / yCKVGDOT FTOYRQPLS AFQAFAiaT SFGIXLACE (SEQ ID N0:7) 91261.doc .13· 1338695 cDNA : 51 101 151 201 251 301 351 401 451 501 551 601 651 701 751 801 851 901 951

AToocmxjr CAOocrroxr στπαχχ3σ aogatooga αοπτγγαο GTITFCATCAG OSAiiAGACAA OGAOOOXX: OGAATOTAA TOGATTOCTC OOOOGTOGAA TATOOOQGOT ICTlOmCAT GTIOTOGAT (OGOCTCAA mnAGOCX; ACATCArrOG TOOOCnOCAG GAGACTCAGG ACC ΟΓΠΧΠ: TCAACGTOOT GATCTAGITA CTTOCXXJro anTICTAAG AAATOACAG AAATCACTCA aJATITOOCr AGATOCIOC πΑΑΟΠΟσ CAAAATOCT

Rroocrrcrr goctcaaatt oocAOGitrr AGAGAcmr ctaatcagtc CITGATAAAG AOGAACAAGA AGACATOVAC (ΠΤΓΓΑ CTO TATCTTOCTC TAACAC3CATC ATTCACTGAT AAOOGAAAGT TTCrPCTOOC OXI3CI3GAOG riTAOGACOC GTOOTACAC TCAGTACATC ATATCACTIX3 ATOCTCATCA

mCICTCAA OGAAGTAATC (ΓΓΑΟΠΠΧί AAAATTAAGA TCAGAimt: TTOOGAGCAA CTITACAGTA TAOGATAOC C AAOCAOCACA CAAOGGAGCA AAACCnCAA ATOOCAAAC3C CACtOJCAGA TTOCATCM AC3CAGATAAG COCTCAACnT OCAGCAOXA ACTTFGAAGr OOCTTCATtHT TCTTATAAAT TCAAGCmT GAAATOAGA OC7POCAAGAA GAATOCTAAG CACACTGCGA TOOOCATCAC CATCAOCnC ATCATCATOC OCIOGACTCT OHOGAOCA AAAOOCATTTr GATCTTAAOCA AOATAATCAA AAAAOOCAAC AAOGATtXTTT OCAOCITGAC AATACTAAAG AACAAAOCTC CTAGATOOCA OGAOCAOTG (ΧΤΓΟΠΧΧΠ * CTCTGAAOT OCATOGACCA (TTrACIUTTG CTTOOGTCAA GAACITPCAG CTOGTOCEA OOCmCAOCA AACTCAACOG ΛΟΟΪΓΓΑΛΑΟ 1001 CCGATGAAGA AACAGTICIT Cr/OGTITG GTAAAGTOOG AGATGACACT 1051 TTCACTATO AmTAGACA OOCTCTCTCT OCATITCAGG αΤΓΠΧΤΑΤ 1101 CK7ICPCACA AGTITOOGCA CTAAACTTOC CTOOGAG (SBQ ID NO: 18)

AtTLP8: polypeptide: POSWIFTP TITITOSYR RMRILLPKQJ fWQIQWKQV QQASKLPLDlf LENKEKIQKL CSR1PHYNKI SKQHEUHD RGRTGLRIOS SVKNFQLTLT EIPRQTllJQM GRVDKARYV [DRYPFSGYQ AFCICUSID SiOJOClV (SEQ ID NO: 8) 1 MAGSRKm LEENK tear imSTQKG EDKENVSPEK VSTSVEm 51 DRALKSQSMKQOTPTEVrNFKSFSTGGRTAlXQSSLQAWSEVDK 101 STOfiOTO VDSEHSSSLK WEFSDSEM PASSWS1LPN RALLOOLPL 151 DVOOCLIV KBQSPBGLSK GSVYSLnHE GRGRKMU VAVHSRRNGK 201 SIFRVAQNVK CU χ χ χ χ χ χ χ χ χ χ χ χ χ χ χ χ χ

TUTOGACACA ATOCAOGGT CrTTATCEAC 1CAAAA0GGA GACX5ATAAOO ΑΟΑΑΐσΐϊιΠ: OOOOOAGAAA GrCTCTAOCr CrGTPOGAAAC TOOGAAACTA

CATOGAGOT TGAACTOCA ATOGA TCAAG OTAACTCTC OGTITOCAAC OGAACHTACA ΑΑΤΠΟΑΑΤ CITOCAAC TOCTOGTOGA ACAfJCTCTGA AOCAGTCATC ACmAOOG TGTATOCAGA AGAACAGTCA OCTTCATAAG ACTAGTITO GAATGAAAAC TTOGACTACrr CnTOTTOG AOCATTCAAG TKXnTGAAA CIGTOOGAGT TTTCOCATIC TGAAOCTOOC OCTOCITGCT

CITOIUTAC TrrOOOCAAC AC3G0CnTCT TCrrOCAAGAC ACTAOnTC GATCTOOGAA GATGCACTTO TOCATTGTC AAAGAACAAT CAOJOAfiOS CriUA (X3CAC OGATCTGfTAT ΑΓΟΟΤΤΑ TAaOVTGAA CXn〇30000C, GTAAAGAOIi C5AA (HTA0CA OTTCCrrAOC ATAiJOCGACG TAATOOGAAA tutatatita 〇QC7roac \ a gaatcitaag oGATiOCitrr οολογγοοοα

TOAAAOTTAT GTOOGTIOCA TCACOOCTAA TUTCTTOGGT TOCAAOTACT AOTATOOGA CAAOUAGrr OGACHTOOT CIOTAGGTAA AATOIXIAAG OGGCTTCm OOGTiOTAAT ATTCACAGOC AOCATAACAA CnTOCAiGG GAOCTACAGA AGAATCAGAA ΟΠΤΟΟΛα: AAAC3CA0CAG OCAATOCAGA AAAACAACAA ΤΑΛΟΟΑ (Χ7ΓΓ CAACAACJCTA OrAAACTAOC GCTTGATroG CTTCAGAATA AGGAAAAAAT TCAGAAQCTA TCOCAAOIA TAOCACATTA CMCAAAATC TOCAAC3CACX: ATGACTITAGA CTICACAGAC AOACJGAAGAA CAOGACTCAG AATACAGAGC TOCTTCAAGA ACrTTCAOCT AAQOCAO; GAGACTOCAA CX3CAGACAAT TCITCAAATC OOGAGAGITG ACAAAOCAAG ATAOTAATC GACITCAGCFr ATOCATTCTC AC33CTAOCAA OCATTCTOC A TTTOCTrOOC TTCTATTCAT TOCAAGOTT CTIOTACTOT T (SBQ ID NO: 19&gt;

AtTLP9 : #peptide:

l WIFRSLLQEM RSRPHRWHA AASTANSSDP FSWSELPEa LRBIURVET 51 VDGGDWPSRR NWACAGVCR SWRILTXEIV AVPEFSSKLT FPISUCQSGP 101 RDSLVQGFIfC RNRKTQSYHL YLGLTrSLTD NOTJUASK UCRATCTim 151 [SLRSDDISK RSNAYLG_ SNFLCTKFTV raSQTCMIC MQKSRSSNFi 々2dl KVSPRVPQGS YPIAHISYEL NVLGSRCPRR fcRCIMDTIPM SIVESRCWA 251 STSISSFSSR SSPVFRSHSK PIRSNSASCS DSGNNIGDPP IVLSNKAPRW 301 HHQUOON FHGRVIVASV KNPQLVAVSD CEAGOTSERl ILOFOCVCKD 91261.doc -15- 1338695 cDNA : 351 MFTMDYGYPI SAFQAFAia SSFETOIACE {SEQ ID N0:9) 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110

TAC3GC I AOOCT CTT/«jAGAAA TOCTGATTAG (XjTTGAGACr GTOOGOOQjA AAOjTOGTCG CTTCTCCGOG TOCAOCAA OGAGATIUTA OCrOTTOCTC TTOOGTATCT OOCTCAAOCA

ATCAOGTTCC GAAGTITAa CEAOGAMTC GCXjICTACJGC CACAlOOGTICT OT3CTAATAC TTCAGACOCT TTCAOTOCT Ασπασία: CGGAOCTOOC CHTGADOaOS OOGATTOGOC oumcrocTr aoctogaoga AATICrOCK: TAAATIOACr AGAGATICTC TACTTCAATC (ΠΤΑΤΑΑΑΑ OC7TAATOGAA ATACTCAATC OTATCATCTC TATCTO3GAT TAAaAOCTC TTOACOCSAT AAOX3GAACT TKTTCTTOC TOCTTCTAAC CTGAACXDOG CAACTTOCAC TGATTACATC ATCIOTTOC CTICAGAIOGA TATCIOAAG AGAAGCAAOG ΟΓΓΑΙϋΓ TOG GAGAATGAGA TOCAAnTOC TT0C5AACAAA ATTCAOX3TC TITGATOCTA CTCAGAOOOG AOCAOOGAAG AKCAOAAGA OOCUCTCTTC TAATITCATC CTAGAfiTTOC TCAOCAAGT TAOCXCATCE CTCACATITC AAOOTCITAG CXnCrOOOGG AOOGAGWCA ATOdTITOCA AATAOCTATG ACCATCGTOG ACTPOOCGAK AOTAGTAOCT TCAACXTOCA TAAOCTCriT TICCAGTO3G TCATCA.OCAG TCnTAOOTC TCACTCAAAA OCATTOOXA GTAATAGTOC ATCMOTAOC GAOCAOOCA ACAAOCrOOG AGATGCAOCA ΤΓΤΧΠΌΠΠΑ OC AACAAAGC TOCACCXTIOG CATCACJCAOT ΤΑΟΤΠΌΟΤΟ ΟΙΟΠΤΑΜΤ TrOTOTrC GAGTOCACT GOL llOOGl I AAGAACTTTC AOCTHTITOC ACTTAGTGAC TCTGAAOCAG GOCAGACATC TGACAOGATC ΑΤΑΠαΧΠ- TIOCBAAWjr TQOGAAOGAC ATCmTAGCA TOGAHATOG ATATC CUATT ICIUUUIIIC ΑΑΟΟσΠΤΧ TATCIGOCIG AGCAGnTTG AAAOCAGAAT TOOCTOTGAA (SBQ ID N0:20)

ATAODAOTTA TCATOGATAC

AtTLPIO: Polypeptide:

l MSFRC1VQDL RDGPGSLSRR SFDFRLSSLH KGKAQGSSFR EVSSSSDLLS 51 PVIVQTSRWA MJTCLLFDV IKRLEESESN WPARKHWAC ASWCRSWRAM 101 0QB1VLGPEI OGKLTFPVSL KQPGPRDAMI QCFnCRDKSK LTFHLFUXS 151 PAaVBCKF LLSAiamwr ηΠΕΠΙΣΜ) aehisrssns ylgklrsnfl 201 (nxaVYmQ pppktsssal itortsrsrf hsrrvspkvp sgsyniaqit 251 YELNVLCniiG PRRMCIMNS 1PISSLEPCC SVPNS3P EKLV PAPYSLDDSF 301 RSNISFSKSS FDHRSLDFSS SRFSEMGISC OCNEEEASFR PL1LKNKQPR 351 WBQUQCXI nfksvtvas vknpqlvaar QPQPQOTGAA AAFTSAP/ 401 BQDKVILQFG KVCKDMFIND YRYPLSAPQA FAICLSSFDT KLACE

(SBQ ID NO: 10)

cDNA: l ATGntxrrrrc gacxxattct tcaagatitg agagatooct rrooc3AC3crr 51 GrCAAGGAOG ACmOCATT TTAGGCTCTC GAGTCTTCAT ΑΑΑΟΧΐΑΛΑβ im cTCAaocmc ττστπΌοστ gagtattoct catdoogtga icrcrnnOo 151 OCTGTIGATAG TTCAGACAAG TAGATOOOCT AATCTTOCrC CAGAGTTACT 201 CIITCATGTC ATCAAAAGAT TAGACijAAAG TCAGAGTAAT TOCXXTOCAA 251 GAAAACATCT TUTOOCTTGT ULTIUJUI11 GTOOCTrCl lG GAGAOCTATG 301 TCXXAAGAGA TPCmTIOOO CXXTOAAATC TCTOOGAAAC TCACTTTOOC 3SI ItrmOXTC AAACAOCCAG GOCCreXTHGA TOCAATCATT CACTCTTTCA 401 TCAAAAOCJGA TAAATCAAAG CTAACATTFC AOCrrmcr 1 lUI'lTAAGT 451 OOOOCTCTAT TACTOGAGAA TCCXSAAATTT CTTCTTTCAG CTAAAAGAAC 501 TOGTTAGAACr ACTOSAAOCG ACTACATTAT CTCCATOGAT OCTGATAACA 91261.doc -16- 1338695 551 601 65i 701 751 801 851 901 951 ΙΟΟΙ 1051 1 ΙΟΙ 1151 1201 1251 1301

TCTCAAGATC CAOCAACTCT TAOCTOOGAA AGCTCAGATC AAACTTCCTT OCXJACAAAGT TCTTtXTRJTA CGACACX3CAA GCACCAOCAA ACACATCTTC GAOGQCACTT ATCACTGATC GAACAAGOgG AAGCAOGTTT CACTOCAGAC GAGTTTCTCC TAAAGTAOCA TOOGGAAOCT ACAACATTOC TCAAATCAO: TATCAGCTCA AOGTTGTTOOG CACAOOOOOG OCAOGACGAA TOCACTGCA T CATCAACTOC ATOOCAATTT CATCOCTOGA AOCAOGOOOT TCAGTOOCTA ACCAAOGOCA GAAACIXDGTC OCTOCAOCAT ACTCICTOGA OGACTCATTC

OOCAJCTTAACA

TCTOCTTCTC TCTAGATTCT AAGAAGAAOC GACTTTCAGA TT3GCACGAOC AGTTOCAATG AGTTOCAICC CPCAAOCK3AC GAOCAAGACV CACAATOGAC GCTTAAOCAG

GtTAAGAATT AOtyrocAocA AOCTOATTCT TATAGGTATC CTTTOACAOC

CAAATCATCA

CTGGTCTTTG TOCAOCTTGT OCAiOCAOCAA OCMJTTTOGT CATTATOjOC TTTCACCAOC 0CTC30CTCGA AATATOCTOC G ACOACAAOG TAAAGAACAA OCAGOCAAOG AAi l IOGOOG GAOGTGTGAC AOCAGCAAGA CA&lt;XXJOCAOC CAAG TOCAOC TO AAAGTAGOGA AAGATAT GTTTCAGOOG ΤΓΤ GtGAA (SBQ ID NO:21&gt; ΟΤΓΓΤ ΪΑΤΑΤ

AtTLPll:

Polypeptide mrsrphrwh dlaaaaaads tsvssqdyrw seipeellre ilirveaadg 51 101 151 201 251 301 351 OGWPSRRSW ACAGVCRCJWR LLftWEIWVp EISSKLTFPl SLKQPGPRDS LVQCFIKM ^ R [TQSYHLYLG LTNSLTDDC3C FLLAAOCLiCH TTUTDYIISL RSDDMSRRSQ AYVGKVRSNF UTTKFTVFDG NLLPSTCAAK LRKSRSYNPA KVSAKVPLGS YPVAHITYEL NVLGSRGPRK MQCUiOTIPT STMHPQGVAS EPSEFPLLCTr RSTLSRSQSK PLRSSSSHLK ETPLVLSMCT PRWHBQLRCW CLMFHGRVTV ASVKNPQLVA AGASOSSGTC MSPERQSERI UjQFGKVCKD MFIWDYGYPI SAFQAFAICX SSFFIKIACE &lt;SEQ ID N0:1L) cDNA :

ATCCJU1ΙΟΪΑ GAOCOCATOG TUrOGTOCAC GAOCIIUCOj OOOOOOCAOC 51 TCCGGATTOC ACITCIUICT CATOOCMGA TTATOOCTOG TCAGAGATTC 101 CTCAAGAGCT TCTTAOOGAG ATICrGATTC GTGTTGAAOC OOOOGAOiCrr 151 CXXX3GAT0CJC CCTCACGA.CD CACJOCnaTTO ocmriocrc OGGTl'lG-JC G 201 TOOCTOOOGG CTACTTATGA AOGAAACXXn * CGi lUJUXT GAGATCTCTT 251 CTMCFTTGAC TTKXOCATC TCTCTCAAGC AOOCTOGTOC AACX3GATTCA 301 CTOUTTCAAT OCTTTATCAA AOGTAATGGA ATTACOCAAT CATATX ^TCT 351 CTATCTOOGA TTAAOCAACT CrTTAACOCA TCATOOGAAG I I'ITI GCTTO 401 CTOOGTCTAA (jTTOAAOCAC ACAACTTOTA OOGATTACAT TATtnCITTA 451 501 551 601 6St 701 751 801 851 901 951 1001

amCTCATO ATATUTCGAG AAGAAOOCAA OCTTATCTTC OCAAAGTCAG ATOGAACTTC CTAOGAAOGA AATTCACIUT CITIGATOGA AATCTOCTOC CTTCAACOOG AOOGOCAAAG TTCAGAAAGA OOOCATCITA T AATOOOXA AA/ijmCAG CAAAACTTOC TCTTOGAACTT TATOCTCTOj CFCATATCAC

ATATGW3CIG

TTATGGACAC

GAAOCATCAG

TACTTOCTCAG TCCTTGAATr

ocrccrroxA

AGACX3CAGAG

AATC7ICn&gt;G

AATAOCTACA

AGTTTaOCTT

OCATTAOOCA caacaagaca

TOCATOOOOG

OCAOGAOCTA

OGAGOGGATT GAT0CD3000

AOCACAAIOG

ACTCOGTACT

GTAGCTCAAG

OC\CXXjKXJC

TCTTCACACTA

GCTGT0C3CAG

ATATTOCAGT

AOCAAGAAAG A1 AGGCTCAAOG CT

A0GA0CAC3CT ACX3C GOGTCAGTCA TQOC AGOGGA TTOOGAAAGT (330GAAAGAT AGAACTTTCA ATC7TCACOX; 91261.doc -17- 1338695 The invention therefore features an amino acid sequence having at least 70% identity to one of SEQ ID NOs: 1-ll (ie including Any number of polypeptides between 70% and 100%. When expressed in a plant cell, for example, an Arabidopsis cell, the polypeptide can modulate the transcription of the gene in response to environmental stimuli. The polypeptide of the present invention can be used to define its Binding DNA elements, such as: promoters, enhancers, or silencers, which transmit plants to various environmental factors. The polypeptides of the invention can also be used to make anti-AtTLP antibodies ( Single or multiple strains of these antibodies are in turn suitable for detecting the presence and distribution of AtTLP proteins in tissues and organelles, for example, such antibodies can be used to confirm the expression of TULP proteins in transgenic plants. A polypeptide refers to a polypeptide that is substantially free of natural binding molecules, ie, the dry matter has a purity of at least 75% (ie, including any number between 75% and 100%). The degree can be determined by any suitable standard method, such as column chromatography, polypropylene guanamine gel electrophoresis or HPLC analysis.

The identity of the identity between the two amino acid sequences is based on the algorithm of Karlin and Altschul (Proc. Nail. Acad. Sci. USA 87, 2264-2268, 1990), according to Karlin and Altschul (Proc· Natl). Acad. Sci. USA 90, 5 873-5 877, 1993) The modified method determines that these algorithms have been incorporated into the XBLAST program of Altschul et al. (J. Mol, Biol. 215, 403-410, 1990). ). The BLAST protein search method was performed using the XBLAST program with a score of 50 and a word length of 3. If a gap occurs between the two sequences, Gapped BLAST (Nucleic Acids Res. 25, 3389-3402, 1997) as described by Altschul et al., when using BLAST and Gapped BLAST 9126 丨.doc -18-1338695 program is used. That is, it is easy to use the default parameters (defailor pararneters) (for example, XBLAST). See ncbi.nim.nih.g〇v. The invention is further characterized by (1) a nucleic acid sequence encoding the polypeptide of the present invention, and (2) hybridizing or complementary to a probe made of nucleic acid sheet # constituting SEq ID N〇s: 12_22 under highly stringent conditions. Nucleic acid. These nucleic acids are at least j 5 (eg, at least 30, 50, 1 〇〇, 200, 500 or 1 〇〇〇) nucleotide lengths. Hybridization under highly stringent conditions means washing at 45 ° C, 0.5 X SSC, and washing at 45 C, 0.1 X SSC. The nucleic acid of the present invention can be used to determine whether AtTLP mRNA is expressed in tissues or cells. The nucleic acid can be used as a primer in a PCR-based cardiac assay or as a labeled probe in a nucleic acid dot method (for example, Northern blotting). . By isolated nucleic acid is meant that the structure of the nucleic acid differs from any natural nucleic acid or any fragment of a native genomic nucleic acid. Thus the term includes, for example: (a DNA having a partial sequence of a native genomic DNA molecule, but the DNA is not flanked by two coding sequences flanked by the portion of the native genome of the organism; (b) entering the pronucleus a molecule produced by a nucleic acid or eukaryotic vector or a nucleic acid in a genomic DNA; (5) in any any vector or genomic DNA; (c) an isolated molecule, such as a cDNA, a genomic fragment by polymerase chain reaction ( a fragment produced by PCR, or a restriction enzyme cleavage fragment; and a hybrid gene, that is, a recombinant nucleotide sequence encoding a portion of the gene of the fusion protein. ' / The present invention is also characterized by a vector comprising the nucleic acid of the present invention Host cell. The host cell can be E. coli ([灭), yeast, insect, (4) (9) such as: Arabidopsis thaliana) or mammalian cells. The polypeptide of the present invention can be produced using a vector and a host cell to produce 91261.doc -19-1338695. For this purpose, the host cell line is cultured in a medium under conditions which can express the polypeptide and is isolated from the polypeptide.

The vectors and host cells described above can also be used to form a transformant comprising a recombinant nucleic acid encoding a heterologous polypeptide of SEQ ID ΝΟ: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or 11. Plant cells or transgenic plants. The production of such transformed plant cells is carried out by introducing a recombinant nucleic acid encoding the heterozygous conjugate polypeptide into a plant cell and expressing the polypeptide in a cell. The production line of the transgenic plant (1) introduces a recombinant nucleic acid encoding the above-described heterozygous polypeptide into a plant cell; (2) expresses the polypeptide in a cell, and forms a plant with the cultured cell. The transformed plant cell or the transgenic plant is sensitive to environmental factors such as high salinity, pathogenic bacteria and low temperature, and thus can be used as a sensor for detecting and tracking changes in the environment (e.g., soil and air). Also included within the scope of the invention are homozygous transformed plant cells lacking a polypeptide comprising the sequence of SEQ ID NO: 2, 3, 4, 5, 6, 7, 8, 9, 10 or 11 (eg, Nanfen cells) and transgenic plants (eg, Arabidopsis). The transformed plant cell or the transgenic plant is more resistant to salt, low temperature, pathogenic bacteria, oxidative stress or water shortage due to the absence or low content of the polypeptide, and is higher than wild type cells or plants (above at least 30〇/〇, for example: 50%, 90%, 100%, 200%). Furthermore, the present invention is characterized by a method of producing a transformed plant cell and a transgenic plant. Both methods include introducing into a plant cell a nucleic acid that reduces the expression of a gene encoding a polypeptide of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or 11 (eg, T- DNA, antisense RNA, and RNAi). The method of producing the plant further comprises cultivating the plant cell to form a plant. 9l261.d〇c • 20-1338695 The details and accompanying drawings of one or more embodiments of the present invention are shown below. Other features, objects, and advantages of the invention will be apparent from the description and appended claims. [Embodiment] The present invention is based on unpredictable discovery (1) Excessive expression of AtTLPs in Arabidopsis will raise the sensitivity of plants to various environmental factors such as salt, low temperature, oxidative stress or water shortage; and (2) Lack of performance of AtTLPs increases the tolerance of plants to several environmental factors. Thus, one aspect of the invention features a transformed plant cell comprising a recombinant nucleic acid encoding a heterozygous AtTLP. At-pLP proteins suitable for use in the present invention include Arabidopsis AtTLPs 1 -11 and TULPs of other species. The plant cell may be a dicot plant cell (e.g., a tomato cell, a canola cell or a potato cell) or a monocot plant cell (e.g., a rice cell, a wheat cell or a barley cell). The transgenic plant cell of the present invention is produced by introducing a recombinant nucleic acid encoding a heterozygous AtTLP protein into a plant cell and expressing the protein in the cell. A variety of plant cell transformation techniques are known to those skilled in the art and can be found in technical and scientific references. See for example: Weising et al., 1988, Am

Rev·Genet 22:421-477. When a heterologous ^71^ gene is expressed in a plant cell, the gene encoded by the gene and the transcriptional initiation regulatory sequence that is dominant in the transcription of the gene is translated into the plant cell. For overexpression, a constitutive plant promoter can be used. Constitutive promoters are active under most environmental conditions and conditions of cell differentiation. Examples of constitutive promoters include the cauliflower mosaic virus (CaMV) 35S transcription initiation 91061.doc 1338695 region, wherein the 1 or 2'-promoter is derived from T-type Agrobacterium tumefaciens (Jgrohcie HMW 仏we/ac/e/u) DNA, ACT11 and Cat3 promoters are from Arabidopsis (Huang et al, 1996, plant Mol. Biol. 33: 125-139 and Zhong et al, 1996, Mol. Gen. Genet. 251.. 196-203), hard The lipid thiol-thiol-loading protein desaturase gene promoter is derived from Brassica napus (heart «fl/?w) (Solocombe et al. 1994, Plant Physiol. 104:1 167-1 1 76), GPcl and Gpc2 promoter The sub-derived from maize (Martinez et al, 1989, J. Mol. Biol. 208:551-565 and Manjunath et al, 1997, Plant Mol. Biol. 33:97-1 12). Alternatively, a tissue-specific promoter or an inducible promoter can be used to dominate the expression of the AtTLP gene in a particular cell type or under more precise environmental or developmental control. Examples of environmental conditions that may affect the transcription of an inducible promoter include anaerobic, elevated temperature, presence of light, spraying chemicals or hormones or infectious pathogens. Examples of tissue-specific promoters or inducible promoters include the root-specific ANR1 promoter (Zhang and Forde, 1998, Science 279: 407) and the photosynthetic organ-specific RBCS promoter (Khoudi et al., i997, Gene 197:343). . In the case of eve-like g, it should include the polyadenylation region at the 3rd end of the marsh area. The polyadenylation region can be derived from the same gene, a variety of other genes, or from T-DNA. Marker genes can also be included to confer an alternative to plant cells. 5=· For example, the 仏δ-hex gene can be encoded to confer biocide resistance, antibiotic resistance (eg, for kanamycin, G418, bie〇mycin, hygromycin resistant or herbicide anti-91261.doc -22· 1338695 (eg for chlorosulfuron or baxite) Basta) a protein with resistance). A recombinant nucleic acid encoding a heterozygous AtTLP protein can be introduced into the genome of a desired plant host cell using a variety of conventional techniques. For example, the recombinant nucleic acid can be directly introduced into the genomic DNA of the plant cell by, for example, polyethylene glycol precipitation, electroporation, microinjection or impact (e.g., DNA particle gun method). See, for example, Paszkowski et al., 1984, EMBO J. 3:2717-2722, Fromm et al., 1985, Proc. Natl. Acad. Sci. USA 82:5824 and Klein et al., 1987, Nature 327:70-73. Alternatively, the recombinant nucleic acid can be combined with a suitable T-DNA flanking region and introduced into a conventional Agrobacterium tumefaciens host vector. When the cells are infected with bacteria, the virulence function of the Agrobacterium tumefaciens iwwe/aciews host dominates the AtTLP gene and the adjacent markers are embedded in the plant cell DNA. The transformation technology of Agrobacterium tumefaciens media, including the disarming of binary vectors and the use of legal related art learners. See, for example, Horsch et al., 1984, Science 233:496-498; Fraley et al., 1 983, Proc. Natl. Acad. Sci. USA 80:4803; and Gene Transfer to Plants, Potrykus Editor, Springer-Verlag, Berlin , 1995. The presence and set number of the AtTLP gene of the heterozygous zygote in the transgenic plants can be determined by standard methods, for example, the Southern blot method. The expression of the heterozygous AtTLP gene in a transgenic plant can be confirmed by detecting and quantifying the heterozygous AtTLP mRNA or protein in the plant. The resulting transformed plant cells can be used for cultivation to form whole plants. The regeneration technique is based on the operation of certain phytohormones in the tissue culture medium, typically based on the biocide or herbicide marker that has been introduced with the heat shock factor gene in 91261.doc • 23· 1338695. Plants formed from the cultured protoplasts, as described in Evans et al., Protoplasts Isolation and Culture; Handbook of Plant Cell Culture, pp. 124-176, MacMillilan Publishing Company, New York, 1 983) and plant, plant protoplast binding, regeneration (Binding, Regeneration of Plants, Plant Protoplasts, pp. 21-73, CRC Press, Boca Raton, 1985). Regeneration can also be achieved from plant healing tissues, explants, organs or parts thereof. Such regenerative techniques are generally described in Klee et al., 1987, Ann Rev. Plant Phys. 3 8:467-486. Once it is confirmed that the at-type zygote AtTLP gene has been stably introduced into the genome of the transgenic plant, it can be introduced into other plants by the breeding mating method, depending on the species to be mated. One or more standard breeding methods can be used to form the whole plant. In another aspect, the invention features a transformed plant cell that lacks one or more homozygous conjugates of Atixps 1-11. The lack of AtTLp(s) enhances the tolerance of cells to many different environmental factors (eg, high salinity). The production of such transformed cells introduces a gene expression in a plant cell that reduces the encoding of a polypeptide encoding SEq ID 1、: 1, 2, 3, 4, 5, 6, 7, 8, 9, 1 , or &quot; Nucleic acid. The nucleic acid, for example, T_DNA, amisense RNA, or iRNA, can be introduced into cells using one of the standard transformation techniques described above. A stable transformant can be selected using a marker gene, and the selection method is as described above. The whole plant can then be produced from the transformed plant cells, or it can be further mated using conventional breeding techniques to produce homozygous zygote plants. The following specific examples are for illustrative purposes only and are not intended to limit the remainder of the disclosure. 91261.doc • 24- 1338695. Without further elaboration, the skilled artisan can use the present invention to the fullest extent of the present invention. The disclosures of the disclosure are hereby incorporated by reference.

AtTLP family: identification of the family using the tubby common sequence (PfamPFO 1167, Kkyn et al, 1996, Cell 85:281-290 and Noben-Trauth et al, 1996, Nature 380: 534-538) The sequence tag (EST) database of the Nanfen Ααζα/ζα/ζα) and the complete Arabidopsis genomic sequence (The Institute of Genome Research, TIGR), using the multi-sequence BLAST algorithm to determine the chunky gene The position of the sequence with significant similarity in the functional sites (P-value &lt;0.0085) » Search results show that there are 11 types of dwarf gene protein genes in the Arabidopsis genome, called to. The corresponding BAC positions of these 11 genes (The Arabidopsis Information Resource, the hypothetical common sequence (TC) group, the AGI gene gamma, the cDNA GenBank accession number and the predicted protein length (number of amino acids) are summarized in Table 1 below. Table 1. AtTLP family member gene name BAC position TC group AGI CDNA gene code GenBank accession number predicted protein length AtTLp 1 F22K20.1 TC95487 Atlg76900 AF487267 455 AtTLP2 T30D6.21 TC86308 At2gl8280 AY045773 394 AtTLP3 F17A22.29 TC86633 At2g47900 AY045774 406 AtTLP4 F8K4.13 - Atlg61940 - 265 AITLP5 T10P12.9 TC102456 Atlg43640 AY046921 429 AtTLP6 F8G22.1 TC90700 Atlg47270 AF487268 388 AtTLP7 F12M16.22TC88599 Atlg53320 AY092403 379 AtTLP8 T24D18.17 - Atlg 16070 AF487269 397 9l26l.doc •25·

AtTLP9 F24P17.15 TC102624 At3g06380 AF487270 380 AtTLPIO F4F7.13. TC101291 Atlg25280 AF487271 445 AtTLP 11 TIA4.60 - At5gl8680 AY046922 380 1338695 Gene-specific 5' and 3' primers are based on the predicted open reading frame (ORF) sequence and The corresponding ESTs were designed in the database, and then RT-PCR was performed using mRNA from two-week old Arabidopsis seedlings. The method of operation was performed using TRIZOL reagent (Invitrogen Pharmaceuticals), separating the total RNA according to the manufacturer's instructions, followed by the coated oligo (dT) magnetic beads and the PolyATract system (Promega Pharmaceuticals, Madison, WT) to separate the PolyA+-mRNA. The first cDNA was synthesized from 0.5 μg of PolyA+-mRNA using Superscript II RNase® reverse transcriptase (Invitrogen). Then, using the above gene-specific primer pair, the cDNA of each AtTLP gene was amplified from the first strand of cDNA. The PCR conditions were as follows: 3 minutes at 94 ° C; 1 minute at 94 ° C / 1 minute at 55 ° C / 1 minute 30 seconds at 72 ° C for 25 cycles. The PCR product was purified using the QIAquick PCR purification kit (Qiagen medicinal) and subsequently subcultured into the T-easy vector (Promega Pharmaceuticals). These pure lines are confirmed by sequencing. Successful amplification of 1 species of AtTLP cDNAs: AtTLPs 1-3 and AtTLPs 5-11 ° It has been found that except for human 11'1^8 2 and 11 'the cDNA sequence from 1 to 8 1 , 3 and 5-10 The amino acid sequence 歹ij derived from 歹ij is identical to the predicted ORFs in the database. Analysis of the AtTLP2 cDNA sequence revealed that the insertion sequence (intron) 3 was between 708 and 781 bp, and the splicing site predicted by this insertion sequence was at 663 and 766 bp. AtTLP 11 cDNA sequence analysis showed that insert 2 and insert 4 were located at 669-803 bp and 1334-1575 1), respectively, while the computer-predicted insert 2 was at 621-803? , No 91261.doc -26· 1338695 There is a predicted insertion sequence 4. All cDNA sequences obtained by this method were sent to GenBank.

The AtTLPI sequence analysis method uses the derived AtTLP amino acid sequence to search for all known motifs using MOTIF SCANNING (Pagni et al., 2001, Nucleic Acid). Res 29: 148-151). The multiple sequence alignment method was performed using ClustalW (Thomopson et al., 1994). This analysis showed that, in addition to AtTLPs 4 and 8, each AtTLP gene has a fully retained dwarf gene functional site at its C-terminus. Unlike animal TULPs, which have extremely divergent N-terminal sequences, in addition to AtTLP8, each AtTLPs has a conserved F-box domain (51-57 residues) (Pfam PF00646).

The pairwise comparison of AtTLP proteins showed that the similarity of the functional sites of the 倭 fat gene was 30% to 80%. Further analysis of the functional site of the chunky gene revealed two PROSITE features: TUB l (Prosite Accession No. PS01200) and TUB2 motif (Prosite Accession No. PS01201). The TUB 1 and TUB2 motifs are located at the C-terminus of the AtTLP protein and contain 14 and 16 amino acid residues, respectively, both of which are highly retained in TULPs from different organisms. Although AtTLP4 and 8 have no obvious TUB 1 and TUB2 motifs, they can be identified by the motif scanning method for the C-terminal chunky gene function (N-fraction &gt; 15) (Pagni et al., 1993, Nucleic Acids Res 29). : 148-151).

The prominent feature of AtTLPs is the functional part of the dwarf gene. The functional part of the dwarf gene of the TUBBY protein contains three amino acids with positive 91261.doc -27-1338695: R332, R363 and K330, which are considered to determine PI (4,5). ) The combination of P2 (Santagata et al., 2001, Science 292: 2041-2050). The sequence alignment of the AtTLP dwarf gene functional site and the mouse trumple gene (TUBBY) functional site showed that in addition to AtTLPs 4 and 8, each AtTLP has a putative PI (4,5) P2 binding functional site. This indicates that AtTLPs 1-3, 4-7, 8-11 may bind to PT(4,5)P2. It is known that the mouse dull gene (TUBBY) protein is a bipartite transcriptional regulator factor. Its dwarf gene function site has double-strand DNA binding activity, and its N-terminal segment seems to regulate transcription (Boggon et al. , 1999, Science 286: 21 19-2125). In plants, the N-terminal sequence of TULP differs from mammalian TULP, and experimental data show that the AtTLP9-GAL4 DNA-binding functional site fusion protein is unable to activate the transcription of the GAL4 promoter in heterologous systems.

Localization of the AtTLP gene family 舆 Gene structure comparison The chromosomal localization of the smelting genes was performed using Map View (www.arabidopsis.org/servlets/mapper) (Huala et al., 2001. Nucleic Acids Res 29: 102-105). It has been found that this gene is not evenly distributed in chromosomes I, II, III and V. There are 7 genes 4, 5 ' 6, 7, 5 and / 0) on chromosome I, 2 genes and 3) on chromosome II 'the other two genes and / / are located on chromosome π, ΠΙ on. Although most of the ΑΤχ/^ genes are located on chromosome I, local repetitive sequences or gene bundles have not been identified.

AtTLP gene profile The analysis of the A7X cadaver gene was performed by coupling-based RT-PCR. The roots and trunks of the 42-day-old Arabidopsis thaliana growing from the soil 91261.doc •28- 1338695 and the lateral stems, round leaves, flower clusters and green fruit pods. Each gene was selected with a pair of gene-specific primers, and PCR amplification was carried out using 15 ng of the first cDNA synthesized according to the above. Ubiquitin was amplified using the ubiquitin gene primer, UBQ10, (5'-ATTTCTCAAAATCTTAAAAACTT-3' and 5-TGATAGTTTTCC CAGTCAAd) as an internal load standard (Norris et al., 1993, Plant Mol. Biol 21: 895-906). The results showed that A7X/5/, 2, 3, (5, 7, 9, and &quot; expressed in all test organs' had a slight difference in mRNA accumulation. Conversely, 5 was mainly expressed in roots, flowers and pods. The organ specificity of Α7ΧΡ5 and 5 is not specific to the specific role of a particular official. Although the performance of AiTLPJ, 2, 3, 6, 7, 9, 0 and JI appears in all experimental organizations, It is still impossible to rule out the possibility that the expression of these genes has cell-type specificity. The performance of these A7X cadaveric genes may differ only when the plant's internal development plan is changed or stimulated by specific environmental factors. This hypothesis searches for the published Arabidopsis Functional Genome Association (AFGC) microarray performance database (the Stanford Microarray Database, genomone-www5.stanford.edu/MicroArray/SMD/) (Wu et al., 2001, Plant Physiol). Biochem 39: 917-926). Double performance is used as the difference decision value. According to the search results, the corresponding DNA expression patterns of 7, 7, and Sichuan are summarized in Table 2. The H3 AtTLP gene is present. Microarray analysis

Chi Ch2 Ch2/Chl Standardization

_ Description __AtTLP2 AtTLP7 AtTLP9 AtTLPlQ Hormone effect ^ auxin msg seedlings not in msg seedlings 10uM 0.32 2.21 reaction IAA 30 minutes 91261.doc -29- 1338695 growth hormone inducement treatment NAA treatment 0.46 2.22 guide Columbia roots Columbia roots cytokinin Control group 15 minutes Cell fraction 2.19 Reactive lysin treatment Abscisic acid insensitivity Wild type control Abscisic acid 0.49 2.6 0.35 Sensing group 1 Insensitive 1 mutant edrl mutant Wild type leaf mutant Leaf 2.33 KN1 downstream control group KN1- GR at 0.43 2.78 4.73 Gene Columbia-0 Background Over-expressed pressure Increases atmospheric Columbia leaf Leaves Leaf 0.33 C〇2 Effect 360ppm C02 1 000 ppm C〇2 C!s8 0.22 for low temperature treatment with low temperature and low temperature resistance 0.15 Receptive gene Columbia wild-type tissue mutant tissue involved in potassium nutrition gene [K+]=120uM [K+]=2mM 0,34 0.2 recorded control group 10uM cadmium treatment 2.72 photo signal 24 hours rhythm time = 12,0 time = 0 hour time = 12 hours 0.37 to light stimulation Seedlings in dark «ρ/ζ^-2 seedlings were grown in black 2.08, exposed to dark growth, exposed to blue light for 1 hour under blue light for 1 hour • Protein enters chloroplast CIA-2 wild type da-2 (mutant strain) 3.46 Consumption of chloroplasts exposed to 230uE exposed to 230uE at 0.3u prime, 2 days after 2 days after identification, cchJ leaves due to WT leaves

91261.doc -30- 1338695 Pressure Increases the Influence of Columbia Leaf C〇2 in the Atmosphere 360ppm C〇2 Columbia Leaf (X33 1000 Ppm C〇2 Responsive for low temperature and low temperature resistance, Columbia wild type tissue, low temperature treatment 0.22 Mutant tissue 0.15 involved in potassium nutrition [K+] = 120 uM gene [K+] = 2 mM 0.34 0.2 cadmium control group 10 uM tin treatment 2.72! These data were obtained from http://afgc.Stanford, edu/afgc_html/site2,htm k All data correspond to a fluorescence intensity greater than 500 on both channels, a normalized ratio of ch2/chl &gt; 2.0 or &lt; 0.5 L. When blast is searched for dbEST, the Arabidopsis EST is found to be equivalent to the micro-array produced by AFGC. The four fragments represented by the data. A7XP2 is equivalent to EST pure line 289Β10Τ7 and 173Κ22Τ7.Α7ΧΡ7, and smelting; TLPchuan is equivalent to EST pure lines 173G1T7, 201E19T7 and F3E6T7 respectively. The results show that 'factors such as hormonal fluctuations and environmental stimuli can regulate four The performance of the genes. As shown in Table 2, the four genes have different responses to different hormone treatments. The transient increase in gene expression is more than twice that of the cytokinin-treated group, but I AA treatment decreased to one-third. This indicates that cytokinin and auxin play an antagonistic role in regulating A7XP2 gene expression. Another cytokinin-related experiment aims to identify genes downstream of the ruler. Its overexpression can upregulate cytokinin production, resulting in delayed senescence in 91261.doc 31 1338695 (Vollbrecht et al., 1991, Nature 350: 241-243). ΑΠ/ν and /0 are expressed in overexpressed transgenic genes. Plants are up-regulated and down-regulated in A7XP2. The different responses of A7XP7, 2, and 70 to guanidine treatment are also noteworthy. In abscisic acid-insensitive 1 mutants (Pei et al., 1997, Plant Cell 9) : 409-423), with a 2 to 3 times decrease in performance, but the performance is more than 2 times. Interestingly, 'the corpse 2 and the corpse 7 are treated with auxin, and the abscisic acid insensitive 1 mutant It is contrary to the performance of the transgenic plants overexpressed by the ruler W. Therefore, the two physiques may antagonize each other in the function of regulating the phytohormone signal pathway. The degree of expression of Α7ΧΡ2 is in the dr/(enhanced disease resistance 1) mutant leaf The Ζ5 gene encodes a MAP kinase similar to that of C77?7, a negative regulator of the ethylene reaction in Arabidopsis (Frye et al., 2001, Proc. Nat. Acad. Sci. 98: 373-378) ). The dr/mutation of Arabidopsis thaliana also confers resistance to mildew disease (Frye and Innes, 1998, Plant Cell 10: 947-956). Therefore, the regulation of A7XP2 gene expression may be related to salicylic acid induction and ethylene defense machine turnover. Environmental stress also affects the performance of the AtTLP gene. For example, similar to the low temperature treatment of ds8 mutant strain, increasing the concentration of CO 2 will inhibit the performance of corpse 2 . The lack of K+ will increase the performance of A7XP7 and Sichuan by 3 times and 5 times respectively. Heavy metal cadmium treatment will stimulate performance. All in all, the performance data of these four Χ7 Χ 基因 genes showed that they were involved in phytohormone and environmental stress signals.

Interaction between At_I_LP9 and ASK1 926-1.doc -32- 1338695 When performing the same search in the published database, it was found that TULPs also appeared in a variety of plant species, including Qingping (Z^w/ια, rice (Ογα saiivfl) Chickpea (Cz_cer ar/en'wMW), maize and Arabidopsis. Unlike the highly divergent N-terminal sequence of animal TULPs, the N-terminus of plant TULPs has a conserved F-box functional segment. By AtTLP, TIR, When the F-box functional segment of UFO and C0I1 is sequenced with the human F-box protein Skp2, it is found that the retained blocks are separated by regions of weak homology, and many of the remaining residues correspond to their known pairs of Skp. The binding is of importance (Schulman et al., 2000, Nature 408: 381-386 and Zheng et al., 2002, Nature 416: 703-709). The F-box functional site was first discovered in the cyclin F, Interacts with the proteins SKP1 and Cdc53 (Cullin) to form a so-called SCF complex. The F-box protein recognizes specific proteins (eg, transcriptional activators or repressors) and acts on the protein breakdown of the ubiquitin-mediated 26S proteosome Analysis Arabidopsis genome shows that Arabidopsis has 21 similar Skpl (or ASK) proteins, which have different performance patterns, ASK1 involves vegetative growth and reproductive development (Zhao et al., 2003, Plant Physiology 133: 203-217)..

To test whether AtTLP interacts with ASK1, AtTLP9 was examined by yeast two-hybrid assay. The C-terminal GAL4AD and BD fusions were constructed using the yeast two-hybrid vector, pAD-GAL4-2.1 and pBD-GAL4 Cam (Stratagene, La Jolla, CA), respectively. The 1.1-kb Sall-PstI fragment containing the entire coding region of AtTLP9 was selected to the Sall-PstI position of the pBD-GAL4 Cam vector, and the 480-bp EcoRI-PstI fragment containing the complete coding region of ASK1 (Atlg75950) to pAD- GRI4-2.1 Vector EcoRI-PstI 9126! .doc ·33· 1338695 Location. Use yeast strain YRG-2 [MATa ura3-52 his3-200 ade2-101 lys2-801 trpl-901 leu2-3, 112 gal4-542 gal80-538 LYS2: GALIUAS-GALITATA-HIS3 URA3: (GAL43 x 17mer) -CYCITATA-lacZ ] Converted together with two carriers. Yeast two-hybrid assays were performed according to the manufacturer's recommendations (Stratagene). The results show that AtTLP9 physically interacts with ASK1. Null (Autumn) has been analyzed in order to explore its function in vivo. Both the loss of function method and the overexpression method were used to study their biological roles. To identify T-DNA insert mutants, A7XPP (At3g06380) was used to search the T-DNA performance database of http://signal.salk.edu/cgi-bin)/tdnaexpress. Two strains of αίί/ρΡ T-DNA intercalated mutants (ABRC preserved strains SALK_016678 and 051 138) were identified, respectively, and the β and T3 strains were obtained from the Arabidopsis Biological Resource Center ( Ohio State University, Columbus). In order to reconfirm the position where the T-DNA is inserted in the gene, the primer corresponding to the left border of the T-DNA and the gene-specific primer are used for PCR, and the obtained amplified fragment is used for sequencing. The specific amplifications used are as follows: attlp9-l: N1. 5' -ATGACGTTCCGAAGTTTACTC- 3'; LBal. 5' -TGGTTCACGTAGTGGGCCATC- 3'; attlp9,2: Cl,5' -TTATTCACAGGCAATTCTGGT - 3·; with LBal, 5, -TGGTTCACGTAGTGGGCCATC- 3,. 9126I.doc -34- 1338695 It has been found that αίί/;?Ρ-/ has a Τ-DNA block at the base of 705, and has a block at the 5' end region of the gene. The T-DNA insertion position is the same as the original description in the T-DNA performance database. However, the T-DNA insertion position is in the promoter region and is not predicted in the expression sequence 1 as the database (the latter is supported by a possible full-length cDNA, equivalent to At3g06380 generated by RIKEN, accession number BT004092 ). The Southern dot method was performed using the "sound//tag gene" to determine the number of T-DNA blocks in the knockout mutant strain in P-2. It has been found that there are 1 and 3 T-DNA blocks in the T4 βίί/ρΡ-/ and T-DNA insert mutants, respectively. The homozygous zygote assay with αί///?9-2 plants was carried out using the method of the method of the colistin selection and PCR. In the case of phenotypes, detailed analysis was performed using a zygote sigmoid cell line. Produce over-expressed transgenic plants. The Xbal-Smal fragment of a few corpses 9 was introduced into the Xbal-Smal position of the ρΒΙ121 vector (Clontech) to generate the 35S::AtTLP9 sense construct. The Xbal-Smal fragment contains the entire A7XP9 coding region and is under the control of the 35S promoter of the cauliflower mosaic virus. The construct was introduced into Agrobacterium strain LBA4404 by electroporation, and transformed into wild-type plants by flower dip method (Clough et al., 1998, Plant J 16: 735-743), after screening. 38 independent transgenic cell lines were obtained (ΤΟ. Analysis of 7 independent homozygous zygote cell lines from T3 sensed transgenic plants showed that the cell lines each contained a set of transfer genes. Two of them were independently transformed. The transcripts of Α7Χ 99 of the clonal cell lines (S13-2 and S16-1) appeared 91461.doc •35· 1338695, and the phenotype was analyzed by 813_2 and 816_1 □3 homozygous zygote cell lines. The method of seed surface sterilization is sterilized by 70% ethanol for 30 seconds, then treated with 6% hydrazine for 5 minutes with bleach, and then washed 5 times with sterile water. For aseptic growth, it is sown with Murashige and Sk〇〇. g salts (Invitr〇gen), vitamins (Duchefa Pharmaceuticals), 7%.7% plant phyt〇agar (Invitrogen) and 1% sucrose solid medium were then transferred to the tissue culture chamber. In the soil When growing, after the seeds are germinated for 8. 1 day, the seedlings are moved to individual pots and cultured in the growth chamber. When grown under aseptic conditions or in soil, the plant growth conditions are maintained at 22 hr, 16 hours light / 8 Under the dark light cycle, the general development and growth phenotype of the plant with the knockout gene is similar to that of the wild type plant. However, when the seed is cultured on the nutrient medium of the amaranth, the germination time of the mutant strain and the seed is earlier than that of the wild type plant. For several hours, the germination time of the selected sense line seeds (ie, S13-2 and S16-1) was later than that of the vehicle control group. It has been found that 50% of wild-type seeds are about 36 after sowing. On the other hand, 50% (10)//^-/ and (10) plant-removed plant seeds germinate at 26-28 hours after sowing, and 50% of S13-2 and S16-1 seeds are 4〇_42 hours after sowing. When germination. 4gA on the mutant _ strain and excessive sputum. Cell germination seed germination rate Axillary lucidum known seed germination for many signals, for the integration of the developmental regulators of various stages of specificity and hormone signal interaction Results 91261.doc - 36 - 1338695 (Finkelstein et al., 2002, Curr. Opin. Plant. Biol. 5: 26-32). The most important hormone known to promote embryonic maturation and prevent germination is aba. To determine whether a transgenic plant changes the seed pair. In response to the sputum, the above transplanted plants were germinated on a medium containing different concentrations of mash. The seeds used are seeds collected at the same time or at similar times. After the surface of the seed was sterilized, the sterile seeds were suspended in 〇·15% amaranth and kept at 4. (: 3 days in the dark to break the dormant period. Then seed the seed on a 2 cm culture dish on a plate of vegetables, which contains 0, 0.25, 0.5, 0.75 or 1.0 μΜ, each of which is divided into 7 plots, each 50 WT and transgenic seeds were placed in the area. When the radicle broke through the seed coat, it was considered as germination. In the presence of 1 μΜ ,, the germination time of the seeds of the sense cell line was later 'the germination rate fell below 10%” On the contrary, the germination rate of the seeds with the deduction ^^ρ_2 mutant is almost 50%, and about 30% of the wild-type seeds can be germinated in the presence of 1 μΜ. These results show that the disruption of genes affects the external factors of the seeds. Sensitivity. In addition to reducing the germination rate of the seed, it also inhibits the growth of the green cotyledon of the positive transgenic cell line. In the MS acacia medium containing i μΜ8-8 and i% sucrose, most of the positive cell lines are 1 day old. The seedlings developed and stopped. On the contrary, under the same conditions, the plants continued to grow, and about 45% of the seedlings continued to develop true leaves, but the speed was slower than that of the sputum mutants. These results showed that the smelting 7X7^ regulatable plants Germination of seeds Sensitivity to early seedling development during the development of the seedlings. ^111££Performed as a temporary adjustment to the sputum during the aspiration process. Quantitative real-time PCR test was used to test the Liao/^ gene in seed maturity, species 91261.doc •37 - 1338695 Performance of germination and early development stages. Using genes as an intrinsic control group (Norris et al., 1993, Plant, Mol. Biol. 21: 895-906). Primer design using Primer Expression 1.0 software (Applied Biosystems) Company). The primers used are:

AtTLP9 forward primer, 5'-TAGGCCACACCGTGTAGTTCA-3·;

AtTLP9 reverse primer, 5,-CGTCAACAGTCTCAACCCTAATCA-3, UBQ10 forward primer, 5'-AGAAGTTCAATGTTTCGTTTCATGTAA-3'; UBQ10 reverse primer, 5LGAACGGAAACATAGTAGAACAC^TATTCA-3,. The real-time quantitative PCR was carried out using a reaction mixture of 500 ng of the first strand of cDNA and 2.5 μM of each primer in lxSYBR Green PCR Master Mix (Applied Biosystems). The PCR cycle was carried out at 50 ° C for 2 minutes, at 95 ° C for 10 minutes, and then repeated at 95 ° C for 15 seconds / 60 t: 1 minute cycle 40 times. The UBQ10 mRNA usage was set at 1, and the AtTLP9 expression gas was measured relative to the control sample. The cycle threshold was determined by the sequence detection system V. 1, 7a software (Applied Biosystems). The product was quantified by the ABI PRISM 7700 Sequence Detection System (Applied Biosystems, Scoresby, Victoria, Australia). Seed germination is divided into three phases: aspiration period, increased metabolic activity, and onset of growth (Bewley. 1997, Plant Cell 9: 1055-1066). The experimental results show that the amount of performance is quite low during seed ripening and seed soaking period of 72 hours. When the seeds were further cultured by moving to 22 ° C, the content rose after 8 hours and reached a peak at 16 hours, but the roots rapidly decreased after 24 hours, and then it was difficult to detect. Other Embodiments 91261.doc -38· 1338695 All features disclosed in this specification may be incorporated in any combination. The features disclosed in this specification can be replaced by another feature that provides the same, equivalent, and similar purpose. Therefore, unless otherwise stated, the features of the two are only for the general description of the equivalent or similar features. A person skilled in the art can understand the basic characteristics of the present invention from the above description, and various changes and modifications can be made without departing from the spirit and scope of the invention. Therefore, other specific embodiments are also within the scope of the following patent application. 91261.doc 39- 1338695 Sequence Listing &lt;110&gt; Academia Sinica &lt;120&gt; Plants and other chunky gene (TULP) family &lt;130&gt; 08919-099001 &lt;140&gt; TW 093118382 &lt;141&gt; 2004-06- 24 &lt;150&gt; US 60/441,380 &lt;151&gt; 2004-01-21 &lt;160&gt; 48

&lt;170&gt; FastSEQ for Windows Version 4.0

&lt;210&gt; 1 &lt;211&gt; 450 &lt;212&gt; PRT &lt;213&gt; 拟南齐 Arabidopsis sp, , &lt;400&gt;

Met Ser Phe Arg Ser lie Val Arg Asp Val Arg Asp Ser lie Gly Ser 15 10 15

Leu Ser Arg Arg Ser Phe Asp Phe Lys Leu Ser Ser Leu Asn Lys Glu 20 25 30

Gly Gly Lys Ser Arg Gly Ser Val Gin Asp Ser His Glu Glu Gin Leu 35 40 45

Val Val Thr lie Gin Glu Thr Pro Trp Ala Asn Leu Pro Pro Glu Leu 50 55 60

Leu Arg Asp Val lie Lys Arg Leu Glu Glu Ser Glu Ser Val Trp Pro 65 70 75 80

Ala Arg Arg His Val Val Ala Cys Ala Ser Val Cys Arg Ser Trp Arg 85 90 95

Asp Met Cys Lys Glu lie Val Gin Ser Pro Glu Leu Ser Gly Lys lie 100 105 110

Thr Phe Pro Val Ser Leu Lys Gin Pro Gly Pro Arg Asp Ala Thr Met 115 120 125

Gin Cys Phe lie Lys Arg Asp Lys Ser Asn Leu Thr Tyr His Leu Tyr 130 135 140

Leu Cys Leu Ser Pro Ala Leu Leu Val Glu Asn Gly Lys Phe Leu Leu 145 150 155 160

Ser Ala Lys Arq lie Arg Arg Thr Thr Tyr Thr Glu Tyr Val lie Ser 165 170 175

Met His Ala Asp Thr lie Ser Arg Ser Ser Asn Thr Tyr lie Gly Lys 180 185 190 lie Arg Ser Asn Phe Leu Gly Thr Lys Phe lie lie Tyr Asp Thr Gin 195 200 205

Pro Ala Tyr Asn Ser Asn lie Ala Arg Ala Val Gin Pro Val Gly Leu 210 215 220

Ser Arg Arg Phe Tyr Ser Lys Arg Val Ser Pro Lys Val Pro Ser Gly 9126).doc 1338695 225 230 235 240

Ser Tyr Lys lie Ala Gin Val Ser Tyr Glu Leu Asn Val Leu Gly Thr 245 250 255

Arg Gly Pro Arg Arg Met His Cys Ala Met Asn Ser lie Pro Ala Ser 260 265 270

Ser Leu Ala Glu Gly Gly Thr Val Pro Gly Gin Pro Asp lie lie Val 275 280 285

Pro Arg Ser lie Leu Asp Glu Ser Phe Arg Ser lie Thr Ser Ser Ser 290 295 300

Ser Arg Lys lie Thr Tyr Asp Tyr Ser Asn Asp Phe Ser Ser Ala Arg 305 310 315 320

Phe Ser Asp He Leu Gly Pro Leu Ser Glu Asp Gin Glu Val Val Leu 325 330 335

Glu Glu Gly Lys Glu Arg Asn Ser Pro Pro Leu Val Leu Lys Asn Lys 340 345 350

Pro Pro Arg Trp His Glu Gin Leu Gin Cys Trp Cys Leu Asn Phe Arg 355 360 365

Gly Arg Val Thr Val Ala Ser Val Lys Asn Phe Gin Leu lie Ala Ala 370 375 380

Asn Gin Pro Gin Pro Gin Pro Gin Pro Gin Pro Gin Pro Gin Pro Leu 385 390 395 400

Thr Gin Pro Gin Pro Ser Gly Gin Thr Asp Gly Pro Asp Lys lie lie 405 410 415

Leu Gin Phe Gly Lys Val Gly Lys Asp Met Phe Thr Met Asp Phe Arg 420 425 430

Tyr Pro Leu Ser Ala Phe Gin Ala Phe Ala lie Cys Leu Ser Ser Pl^e 435 440 445

Asp Thr 450

&lt;210&gt; 2 &lt;211&gt; 394 &lt;212&gt; PRT &lt;213> Arabidopsis Arabidopsis sp. &lt;400&gt; 2

Met Ser Leu Lys Ser lie Leu Arg Asp Leu Lys Glu Val Arg Asp Gly 1 5 10 15

Leu Gly Gly He Ser Lys Arg Ser Trp Ser Lys Ser Ser His lie Ala 20 25 30

Pro Asp Gin Thr Thr Pro Pro Leu Asp Asn lie Pro Gin Ser Pro Trp 35 40 45

Ala Ser Leu Pro Pro Glu Leu Leu His Asp lie lie Trp Arg Val Glu 50 55 60

Glu Ser Glu Thr Ala Trp Pro Ala Arg Ala Ala Val Val Ser Cys Ala 65 70 75 80

Ser Val Cys Lys Ser Trp Arg Gly lie Thr Met Glu lie Val Arg lie 85 90 95

Pro Glu Gin Cys Gly Lys Leu Thr Phe Pro lie Ser Leu Lys Gin Pro 100 105 110

Gly Pro Arg Asp Ser Pro lie Gin Cys Phe lie Lys Arg Asn Arg Ala 115 120 125

Thr Ala Thr Tyr lie Leu Tyr Tyr Gly Leu Met Pro Ser Glu Thr Glu 130 135 140

Asn Asp Lys Leu Leu Leu Ala Ala Arg Arg lie Arg Arg Ala Thr Cys 145 150 155 160

Thr Asp Phe lie lie Ser Leu Ser Ala Lys Asn Phe Ser Arg Ser Ser 9I261.doc 1338695 165 170 175

Ser Thr Tyr Val Gly Lys Leu Arg Ser Gly Phe Leu Gly Thr Lys Phe 180 185 190

Thr He Tyr Asp Asn Gin Thr Ala Ser Ser Thr Ala Gin Ala Gin Pro 195 200 205

Asn Arg Arg Leu His Pro Lys Gin Ala Ala Pro Lys Leu Pro Thr Asn 210 215 220

Ser Ser Thr Val Gly Asn lie Thr Tyr Glu Leu Asn Val Leu Arg Thr 225 230 235 240

Arg Gly Pro Arg Arg Met His Cys Ala Met Asp Ser lie Pro Leu Ser 245 250 255

Ser Val He Ala Glu Pro Ser Val Val Gin Gly He Glu Glu Glu Val 260 265 270

Ser Ser Ser Pro Ser Pro Lys Gly Glu Thr lie Thr Thr Asp Lys Glu 275 280 285

He Pro Asp Asn Ser Pro Ser Leu Arg Asp Gin Pro Leu Val Leu Lys 290 295 300

Asn Lys Ser Pro Arg Trp His Glu Gin Leu Gin Cys Trp Cys Leu Asn 305 310 315 320

Phe Lys Gly Arg Val Thr Val Ala Ser Val Lys Asn Phe Gin Leu Val

325 330 335

Ala Glu lie Asp Ala Ser Leu Asp Ala Pro Pro Glu Glu His Glu Arg 340 345 350

Val lie Leu Gin Phe Gly Lys lie Gly Lys Asp lie Phe Thr Met Asp 355 360 365

Tyr Arg Tyr Pro Leu Ser Ala Phe Gin Ala Phe Ala lie Cys lie S^r 370 375 380

Ser Phe Asp Thr Lys Pro Ala Cys Glu Gly 385 390

&lt;210&gt; 3 &lt;211&gt; 406 &lt;212&gt; PRT &lt;213> Nannanfen Arabidopsis sp. &lt;400&gt; 3

Met Ser Phe Lys Ser Leu lie Gin Asp Met Arg Gly Glu Leu Gly Ser 15 10 15 lie Ser Arg Lys Gly Phe Asp Val Arg Phe Gly Tyr Gly Arg Ser Arg 20 25 30

Ser Gin Arg Val Val Gin Asp Thr Ser Val Pro Val Asp Ala Phe Lys 35 40 45

Gin Ser Cys Trp Ala Ser Met Pro Pro Glu Leu Leu Arg Asp Val Leu 50 55 60

Met Arg lie Glu Gin Ser Glu Asp Thr Trp Pro Ser Arg Lys Asn Val 65 70 75 80

Val Ser Cys Ala (?ly Val Cys Arg Asn Trp Arg Glu lie Val Lys Glu 85 90 95 lie Val Arg Val Pro Glu Leu Ser Ser Lys Leu Thr Phe Pro lie Ser 100 105 110

Leu Lys Gin Pro Gly Pro Arg Gly Ser Leu Val Gin Cys Tyr He Met 115 120 125

Arg Asn Arg Ser Asn Gin Thr Tyr Tyr Leu Tyr Leu Gly Leu Asn Gin 130 135 140

Ala Ala Ser Asn Asp Asp Gly Lys Phe Leu Leu Ala Ala Lys Arg Phe 145 150 155 160

Arg Arg Pro Thr Cys Thr Asp Tyr lie lie Ser Leu Asn Cys Asp Asp 91261.doc 1338695 165 170 175

Val Ser Arg Gly Ser Asn Thr Tyr lie Gly Lys Leu Arg Ser Asn Phe 180 185 190

Leu Gly Thr Lys Phe Thr Val Tyr Asp Ala Gin Pro Thr Asn Pro Gly 195 200 205

Thr Gin Val Thr Arg Thr Arg Ser Ser Arg Leu Leu Ser Leu Lys Gin 210 215 220

Val Ser Pro Arg lie Pro Ser Gly Asn Tyr Pro Val Ala His lie Ser 225 230 235 240

Tyr Glu Leu Asn Val Leu Gly Ser Arg Gly Pro Arg Arg Met Gin Cys 245 250 255

Val Met Asp Ala lie Pro Ala Ser Ala Val Glu Pro Gly Gly Thr Ala 260 265 270

Pro Thr Gin Thr Glu Leu Val His Ser Asn Leu Asp Ser Phe Pro Ser 275 280 285

Phe Ser Phe Phe Arg Ser Lys Ser lie Arg Ala Glu Ser Leu Pro Ser 290 295 300

Gly Pro Ser Ser Ala Ala Gin Lys Glu Gly Leu Leu Val Leu Lys Asn 305 310 315 320

Lys Ala Pro Arg Trp His Glu Gin Leu Gin Cys Trp Cys Leu Asn Phe 325 330 335

Asn Gly Arg Val Thr Val Ala Ser Val Lys Asn Phe Gin Leu Val Ala 340 345 350

Ala Pro Glu Asn Gly Pro Ala Gly Pro Glu His Glu Asn Val He Leu 355 360 365

Gin Phe Gly Lys Val Gly Lys Asp Val Phe Thr Met Asp Tyr Gin Tyr 370 375 380 '

Pro lie Ser Ala Phe Gin Ala Phe Thr lie Cys Leu Ser Ser Phe Asp 385 390 395 400

Thr Lys ILe Ala Cys Glu 405

&lt;210&gt; 4 &lt;211&gt; 265 &lt;212&gt; PRT &lt;213&gt; 拟南齐 Arabidopsissp. &lt;_&gt; 4

Met Pro Pro Glu Leu Leu Arg Asp Val Leu Met Arg lie Glu Arg Ser 15 10 15

Glu Asp Thr Trp Pro Ser Arg Lys Asn Val Val Ser Cys Val Gly Val 20 25 30

Cys Lys Asn Trp Arg Gin lie Phe Lys Glu lie Val Asn Val Pro Glu 35 40 45

Val Ser Ser Lys Phe Thr Phe Pro lie Ser Leu Lys Gin Pro Gly Pro 50 55 60

Gly Gly Ser Leu Val Gin Cys Tyr Val Lys Arg Asn Arg Ser Asn Gin 65 70 75 80

Thr Phe Tyr Leu Tyr Leu Gly Gly Glu Ala Lys lie Phe Cys Gin Ser 85 90 95

Glu Pro Ser Asp lie Tyr Leu Val Pro Tyr Ser Tyr Arg Glu Thr His 100 105 110

Cys Val Met Asp Ala lie Ser Ala Ser Ala Val Lys Pro Gly Gly Thr 115 120 125

Ala Thr Thr Gin Thr Glu Leu Asp Asn Phe Val Ser Phe Arg Ser Pro 130 135 140

Ser Gly Gin Lys Glu Gly Val Leu Val Leu Lys Ser Lys Val Pro Arg 4-

91261.doc 1338695 145 150 155 160

Leu Glu Glu Gin Ser Trp Cys Leu Asp Phe Asn Gly Ser Pro Glu Asn 165 170 175

Glu Pro Glu Asn Glu Asn Asp lie Phe Gin Phe Ala Lys Val Gly Asn 180 185 190

Leu His Lys Leu Phe Ser Leu Tyr Glu Ala Glu Trp lie Pro Leu Val 195 200 205

Arg Thr Ser Val Phe Ala Val lie Ala Arg Val Cys Arg Asp Lys Lys 210 215 220

His Thr Pro Ser Tyr Glu Leu Lys Leu Ala Leu Tyr Phe Ala Lys Asn 225 230 235 240

Ser Ala lie Leu Lys Lys Phe Val Leu Arg Gly Tyr Thr Arg Glu Glu 245 250 255

Asp Leu Leu Ala Leu Pro Val Ala Asn 260 265

&lt;210&gt; 5 &lt;211&gt; 429 &lt;212&gt; PRT &lt; 213 &gt; 拟南齐 Arabidopsissp.

&lt;400&gt; 5

Met Ser Phe Leu Ser lie Val Arg Asp Val Arg Asp Thr Val Gly Ser 1 5 10 15

Phe Ser Arg Arg Ser Phe Asp Val Arg Val Ser Asn Gly Thr Thr His 20 25 30 ,

Gin Arg Ser Lys Ser His Gly Val Glu Ala His He Glu Asp Leu lie 35 40 45

Val lie Lys Asn Thr Arg Trp Ala Asn Leu Pro Ala Ala Leu Leu Arg 50 55 60

Asp Val Met Lys Lys Leu Asp Glu Ser Glu Ser Thr Trp Pro Ala Arg 65 70 Ί5 80

Lys Gin Val Val Ala Cys Ala Gly Val Cys Lys Thr Trp Arg Leu Met 85 90 95

Cys Lys Asp lie Val Lys Ser Pro Glu Phe Ser Gly Lys Leu Thr Phe 100 105 110

Pro Val Ser Leu Lys Gin Pro Gly Pro Arg Asp Gly lie lie Gin Cys 115 120 125

Tyr lie Lys Arg Asp Lys Ser Asn Met Thr Tyr His Leu Tyr Leu Ser 130 135 140

Leu Ser Pro Ala lie Leu Val Glu Ser Gly Lys Phe Leu Leu Ser Ala 145 150 155 160

Lys Arg Ser Arg Arg Ala Thr Tyr Thr Glu Tyr Val lie Ser Met Asp 165 170 175

Ala Asp Asn lie Ser Arg Ser Ser Ser Thr Tyr lie Gly Lys Leu Lys 180 185 190

Ser Asn Phe Leu Gly Thr Lys Phe lie Val Tyr Asp Thr Ala Pro Ala 195 200 205

Tyr Asn Ser Ser Gin lie Leu Ser Pro Pro Asn Arg Ser Arg Ser Phe 210 215 220

Asn Ser Lys Lys Val Ser Pro Lys Val Pro Ser Gly Ser Tyr Asn lie 225 230 235 240

Ala Gin Val Thr Tyr Glu Leu Asn Leu Leu Gly Thr Arg Gly Pro Arg 245 250 255

Arg Met Asn Cys lie Met His Ser lie Pro Ser Leu Ala Leu Glu Pro 260 265 270

Gly Gly Thr Val Pro Ser Gin Pro Glu Phe Leu Gin Arg Ser Leu Asp 9I261.doc 1338695 275 280 285

Glu Ser Phe Arg Ser lie Gly Ser Ser Lys He Val Asn His Ser Gly 290 295 300

Asp Phe Thr Arg Pro Lys Glu Glu Glu Gly Lys Val Arg Pro Leu Val 305 310 315 320

Leu Lys Thr Lys Pro Pro Arg Trp Leu Gin Pro Leu Arg Cys Trp Cys 325 330 335

Leu Asn Phe Lys Gly Arg Val Thr Val Ala Ser Val Lys Asn Phe Gin 340 345 350

Leu Met Ser Ala Ala Thr Val Gin Pro Gly Ser Gly Ser Asp Gly Gly 355 360 365

Ala Leu Ala Thr Arg Pro Ser Leu Ser Pro Gin Gin Pro Glu Gin Ser 370 375 380

Asn His Asp Lys lie lie Leu His Phe Gly Lys Val Gly Lys Asp Met 385 390 395 400

Phe Thr Met Asp Tyr Arg Tyr Pro Leu Ser Ala Phe Gin Ala Phe Ala 405 410 415

He Ser Leu Ser Thr Phe Asp Thr Lys Leu Ala Cys Glu 420 425

&lt;210&gt; 6 &lt;211&gt; 388 &lt;212&gt; PRT &lt;213&gt; South-South Arabidopsis sp. &lt;400&gt;

Met Ser Leu Lys Asn lie Val Lys Asn Lys Tyr Lys Ala lie Gly Arg 15 10 15

Arg Gly Arg Ser His lie Ala Pro Glu Gly Ser Ser Val Ser Ser Ser 20 25 30

Leu Ser Thr Asn Glu Gly Leu Asn Gin Ser lie Trp Val Asp Leu Pro 35 40 45

Pro Glu Leu Leu Leu Asp lie lie Gin Arg lie Glu Ser Glu Gin Ser 50 55 60

Leu Trp Pro Gly Arg Arg Asp Val Val Ala Cys Ala Ser Val Cys Lys 65 70 75 80

Ser Trp Arg Glu Met Thr Lys Glu Val Val Lys Val Pro Glu Leu Ser 85 90 95

Gly Leu lie Thr Phe Pro lie Ser Leu Arg Gin Pro Gly Pro Arg Asp 100 105 110

Ala Pro lie Gin Cys Phe He Lys Arg Glu Arg Ala Thr Gly lie Tyr 115 120 125

Arg Leu Tyr Leu Gly Leu Ser Pro Ala Leu Ser Gly Asp Lys Ser Lys 130 135 140

Leu Leu Leu Ser Ala Lys Arg Val Arg Arg Ala Thr Gly Ala Glu Phe 145 150 155 160

Val Val Ser Leu Ser Gly Asn Asp Phe Ser Arg Ser Ser Ser Asn Tyr 165 170 175

He Gly Lys Leu Arg Ser Asn Phe Leu Gly Thr Lys Phe Thr Val Tyr 180 185 190

Glu Asn Gin Pro Pro Pro Phe Asn Arg Lys Leu Pro Pro Ser Met Gin 195 200 205

Val Ser Pro Trp Val Ser Ser Ser Ser Serr Tyr Asn lie Ala Ser 210 215 220 lie Leu Tyr Glu Leu Asn Val Leu Arg Thr Arg Gly Pro Arg Arg Met 225 230 235 240

Gin Cys lie Met His Ser lie Pro lie Ser Ala lie Gin Glu Gly Gly -6-

91261.doc 1338695 245 250 255

Lys lie Gin Ser Pro Thr Glu Phe Thr Asn Gin Gly Lys Lys Lys Lys 260 265 270

Lys Pro Leu Met Asp Phe Cys Ser Gly Asn Leu Gly Gly Glu Ser Val 275 280 285 lie Lys Glu Pro Leu lie Leu Lys Asn Lys Ser Pro Arg Trp His Glu 290 295 300

Gin Leu Gin Cys Trp Cys Leu Asn Phe Lys Gly Arg Val Thr Val Ala 305 310 315 320

Ser Val Lys Asn Phe Gin Leu Val Ala Ala Ala Ala Glu Ala Gly Lys 325 330 335

Asn Met Asn lie Pro Glu Glu Glu Gin Asp Arg Val lie Leu Gin Phe 340 345 350

Gly Lys lie Gly Lys Asp lie Phe Thr Met Asp Tyr Arg Tyr Pro lie 355 360 365

Ser Ala Phe Gin Ala Phe Ala lie Cys Leu Ser Ser Phe Asp Thr Lys 370 375 380

Pro Val Cys Glu 385

&lt;210&gt; 7 &lt;211&gt; 379 &lt;212&gt; PRT &lt; 213 &gt; 拟南齐 Arabidopsis sp. &lt;400&gt; 7

Met Pro Leu Ser Arg Ser Leu Leu Ser Arg Arg lie Ser Asn Ser Phe 15 10 15

Arg Phe His Gin Gly Glu Thr Thr Thr Ala Pro Glu Ser Glu Ser lie 20 25 30

Pro Pro Pro Ser Asn Met Ala Gly Ser Ser Ser Trp Ser Ala Met Leu 35 40 45

Pro Glu Leu Leu Gly Glu lie lie Arg Arg Val Glu Glu Thr Glu Asp 50 55 60

Arg Trp Pro Gin Arg Arg Asp Val Val Thr Cys Ala Cys Val Ser Lys 65 70 75 80

Lys Trp Arg Glu He Thr His Asp Phe Ala Arg Ser Ser Leu Asn Ser 85 90 95

Gly Lys lie Thr Phe Pro Ser Cys Leu Lys Leu Pro Gly Pro Arg Asp 100 105 110

Phe Ser Asn Gin Cys Leu lie Lys Arg Asn Lys Lys Thr Ser Thr Phe 115 120 125

Tyr Leu Tyr Leu Ala Leu Thr Pro Ser Phe Thr Asp Lys Gly Lys Phe 130 135 140

Leu Leu Ala Ala Arg Arg Phe Arg Thr Gly Ala Tyr Thr Glu Tyr lie 145 150 155 160 lie Ser Leu Asp Ala Asp Asp Phe Ser Gin Gly Ser Asn Ala Tyr Val 165 170 175

Gly Lys Leu Arg Ser Asp Phe Leu Gly Thr Asn Phe Thr Val Tyr Asp 180 185 190

Ser Gin Pro Pro His Asn Gly Ala Lys Pro Ser Asn Gly Lys Ala Ser 195 200 205

Arg Arg Phe Ala Ser Lys Gin lie Ser Pro Gin Val Pro Ala Gly Asn 210 215 220

Phe Glu Val Gly His Val Ser Tyr Lys Phe Asn Leu Leu Lys Ser Arg 225 230 235 240

Gly Pro Arg Arg Met Val Ser Thr Leu Arg Cys Pro Ser Pro Ser Pro 91261 .doc 245 250 255

Ser Ser Ser Ser Ala Gly Leu Ser Ser Asp Gin Lys Pro Cys Asp Val 260 265 270

Thr Lys lie Met Lys Lys Pro Asn Lys Asp Gly Ser Ser Leu Thr He 275 280 285

Leu Lys Asn Lys Ala Pro Arg Trp His Glu His Leu Gin Cys Trp Cys 290 295 300

Leu Asn Phe His Gly Arg Val Thr Val Ala Ser Val Lys Asn Phe Gin 305 310 315 320

Leu Val Ala Thr Val Asp Gin Ser Gin Pro Ser Gly Lys Gly Asp Glu 325 330 335

Glu Thr Val Leu Leu Gin Phe Gly Lys Val Gly Asp Asp Thr Phe Thr 340 345 350

Met Asp Tyr Arg Gin Pro Leu Ser Ala Phe Gin Ala Phe Ala lie Cys 355 360 365

Leu Thr Ser Phe Gly Thr Lys Leu Ala Cys Glu 370 375

&lt;210&gt; 8 &lt;211&gt; 397 &lt;212&gt; PRT &lt;213&gt; 拟南齐 Arabidopsissp. &lt;400&gt; 8

Met Ala Gly Ser Arg Lys Val Asn Asp Leu Leu Glu Glu Asn Lys Gly 1 5 10 15

Asn Val Asp Thr lie Thr Gly Ser Leu Ser Thr Gin Lys Gly Glu Asp 20 25 30

Lys Glu Asn Val Ser Pro Glu Lys Val Ser Thr Ser Val Glu Thr Arg 35 40 45

Lys Leu Asp Arg Ala Leu Lys Ser Gin Ser Met Lys Gly Asn Ser Gly 50 55 60

Phe Pro Thr Glu Val Thr Asn Phe Lys Ser Phe Ser Thr Gly Gly Arg 65 70 75 80

Thr Ala Leu Lys Gin Ser Ser Leu Gin Ala Cys Met Gin Lys Asn Ser 85 90 95

Glu Val Asp Lys Ser Ser Phe Gly Met Lys Thr Trp Thr Ser Val Asp 100 105 110

Ser Glu His Ser Ser Ser Leu Lys Val Trp Glu Phe Ser Asp Ser Glu 115 120 125

Ala Ala Pro Ala Ser Ser Trp Ser Thr Leu Pro Asn Arg Ala Leu Leu 130 135 140

Cys Lys Thr Leu Pro Leu Asp Val Gly Arg Cys Thr Cys Leu lie Val 145 150 155 160

Lys Glu Gin Ser Pro Glu Gly Leu Ser His Gly Ser Val Tyr Ser Leu 165 170 175

Tyr Thr His Glu Qly Arg Gly Arg Lys Asp Arg Lys Leu Ala Val Ala 180 185 190

Tyr His Ser Arg Arg Asn Gly Lys Ser lie Phe Arg Val Ala Gin Asn 195 200 205

Val Lys Gly Leu Leu Cys Ser Ser Asp Glu Ser Tyr Val Gly Ser Met 210 215 220

Thr Ala Asn Leu Leu Gly Ser Lys Tyr Tyr lie Trp Asp Lys Gly Val 225 230 235 240

Arg Val Gly Ser Val Gly Lys Met Val Lys Pro Leu Leu Ser Val Val 245 250 255 lie Phe Thr Pro Thr lie Thr Thr Trp Thr Gly Ser Tyr Arg Arg Met 9126 丨.doc 1338695 260 265 270

Arg Thr Leu Leu Pro Lys Gin Gin Pro Met Gin Lys Asn Asn Asn Lys 275 280 285

Gin Val Gin Gin Ala Ser Lys Leu Pro Leu Asp Trp Leu Glu Asn Lys 290 295 300

Glu Lys He Gin Lys Leu Cys Ser Arg lie Pro His Tyr Asn Lys lie 305 310 315 320

Ser Lys Gin His Glu Leu Asp Phe Arg Asp Arg Gly Arg Thr Gly Leu 325 330 335

Arg He Gin Ser Ser Val Lys Asn Phe Gin Leu Thr Leu Thr Glu Thr 340 345 350

Pro Arg Gin Thr lie Leu Gin Met Gly Arg Val Asp Lys Ala Arg Tyr 355 360 365

Val lie Asp Phe Arg Tyr Pro Phe Ser Gly Tyr Gin Ala Phe Cys lie 370 375 380

Cys Leu Ala Ser lie Asp Ser Lys Leu Cys Cys Thr Val 385 390 395

&lt;210&gt; 9 &lt;211&gt; 380 &lt;212&gt; PRT &lt;213&gt; Arabidopsis Arabidopsis sp. &lt;400&gt;

Met Thr Phe Arg Ser Leu Leu Gin Glu Met Arg Ser Arg Pro His Arg 1 5 10 15

Val Val His Ala Ala Ala Ser Thr Ala Asn Ser Ser Asp Pro Phe Ser 20 25 30

Trp Ser Glu Leu Pro Glu Glu Leu Leu Arg Glu lie Leu lie Arg Val 35 40 45

Glu Thr Val Asp Gly Gly Asp Trp Pro Ser Arg Arg Asn Val Val Ala 50 55 60

Cys Ala Gly Val Cys Arg Ser Trp Arg lie Leu Thr Lys Glu lie Val 65 70 75 80

Ala Val Pro Glu Phe Ser Ser Lys Leu Thr Phe Pro lie Ser Leu Lys 85 90 95

Gin Ser Gly Pro Arg Asp Ser Leu Val Gin Cys Phe lie Lys Arg Asn 100 105 110

Arg Asn Thr Gin Ser Tyr His Leu Tyr Leu Gly Leu Thr Thr Ser Leu 115 120 125

Thr Asp Asn Gly Lys Phe Leu Leu Ala Ala Ser Lys Leu Lys Arg Ala 130 135 140

Thr Cys Thr Asp Tyr lie lie Ser Leu Arg Ser Asp Asp lie Ser Lys 145 150 155 160

Arg Ser Asn Ala Tyr Leu Gly Arg Met Arg Ser Asn Phe Leu Gly Thr 165 170 175

Lys Phe Thr Val Phe Asp Gly Ser Gin Thr Gly Ala Ala Lys Met Gin 180 185 190

Lys Ser Arg Ser Ser Asn Phe lie Lys Val Ser Pro Arg Val Pro Gin 195 200 205

Gly Ser Tyr Pro lie Ala His lie Ser Tyr Glu Leu Asn Val Leu Gly 210 215 220

Ser Arg Gly Pro Arg Arg Met Arg Cys lie Met Asp Thr lie Pro Met 225 230 235 240

Ser lie Val Glu Ser Arg Gly Val Val Ala Ser Thr Ser lie Ser Ser 245 250 255

Phe Ser Ser Arg Ser Ser Pro Val Phe Arg Ser His Ser Lys Pro Leu 9126l.doc 1338695 260 265 270

Arg Ser Asn Ser Ala Ser Cys Ser Asp Ser Gly Asn Asn Leu Gly Asp 275 280 285

Pro Pro Leu Val Leu Ser Asn Lys Ala Pro Arg Trp His Glu Gin Leu 290 295 300

Arg Cys Trp Cys Leu Asn Phe His Gly Arg Val Thr Val Ala Ser Val 305 310 315 320

Lys Asn Phe Gin Leu Val Ala Val Ser Asp Cys Glu Ala Gly Gin Thr 325 330 335

Ser Glu Arg He lie Leu Gin Phe Gly Lys Val Gly Lys Asp Met Phe 340 345 350

Thr Met Asp Tyr Gly Tyr Pro He Ser Ala Phe Gin Ala Phe Ala lie 355 360 365

Cys Leu Ser Ser Phe Glu Thr Arg lie Ala Cys Glu 370 375 380

&lt;210&gt; 10 &lt;211&gt; 445 &lt;212&gt; PRT &lt;213&gt; Nannanfen Arabidopsis sp. &lt;400&gt;

Met Ser Phe Arg Gly He Val Gin Asp Leu Arg Asp Gly Phe Gly Ser 1 5 10 15

Leu Ser Arg Arg Ser Phe Asp Phe Arg Leu Ser Ser Leu His Lys Gly 20 25 30 .

Lys Ala Gin Gly Ser Ser Phe Arg Glu Tyr Ser Ser Ser Arg Asp Leu 35 40 45

Leu Ser Pro Val He Val Gin Thr Ser Arg Trp Ala Asn Leu Pro Pro 50 55 60

Glu Leu Leu Phe Asp Val lie Lys Arg Leu Glu Glu Ser Glu Ser Asn 65 70 75 80

Trp Pro Ala Arg Lys His Val Val Ala Cys Ala Ser Val Cys Arg Ser 85 90 95

Trp Arg Ala Met Cys Gin Glu He Val Leu Gly Pro Glu He Cys Gly 100 105 110

Lys Leu Thr Phe Pro Val Ser Leu Lys Gin Pro Gly Pro Arg Asp Ala 115 120 125

Met He Gin Cys Phe He Lys Arg Asp Lys Ser Lys Leu Thr Phe His 130 135 140

Leu Phe Leu Cys Leu Ser Pro Ala Leu Leu Val Glu Asn Gly Lys Phe 145 150 155 160

Leu Leu Ser Ala Lys Arg Thr Arg Arg Thr Thr Arg Thr Glu Tyr He 165 170 175 lie Ser Met Asp Ala Asp Asn lie Ser Arg Ser Ser Asn Ser Tyr Leu 180 185 190

Gly Lys Leu Arg Ser Asn Phe Leu Gly Thr Lys Phe Leu Val Tyr Asp 195 200 205

Thr Gin Pro Pro Pro Asn Thr Ser Ser Ser Ala Leu He Thr Asp Arg 210 215 220

Thr Ser Arg Ser Arg Phe His Ser Arg Arg Val Ser Pro Lys Val Pro 225 230 235 240

Ser Gly Ser Tyr Asn He Ala Gin lie Thr Tyr Glu Leu Asn Val Leu 245 250 255

Gly Thr Arg Gly Pro Arg Arg Met His Cys lie Met Asn Ser lie Pro 260 265 270 lie Ser Ser Leu Glu Pro Gly Gly Ser Val Pro Asn Gin Pro Glu Lys 10-

91261.doc 1338695 275 280 285

Leu Val Pro Ala Pro Tyr Ser Leu Asp Asp Ser Phe Arg Ser Asn lie 290 295 300

Ser Phe Ser Lys Ser Ser Phe Asp His Arg Ser Leu Asp Phe Ser Ser 305 310 315 320

Ser Arg Phe Ser Glu Met Gly lie Ser Cys Asp Asp Asn Glu Glu Glu 325 330 335

Ala Ser Phe Arg Pro Leu lie Leu Lys Asn Lys Gin Pro Arg Trp His 340 345 350

Glu Gin Leu Gin Cys Trp Cys Leu Asn Phe Arg Gly Arg Val Thr Val 355 360 365

Ala Ser Val Lys Asn Phe Gin Leu Val Ala Ala Arg Gin Pro Gin Pro 370 375 380

Gin Gly Thr Gly Ala Ala Ala Ala Pro Thr Ser Ala Pro Ala His Pro 385 390 395 400

Glu Gin Asp Lys Val lie Leu Gin Phe Gly Lys Val Gly Lys Asp Met 405 410 415

Phe Thr Met Asp Tyr Arg Tyr Pro Leu Ser Ala Phe Gin Ala Phe Ala 420 425 430

Lie Cys Leu Ser Ser Phe Asp Thr Lys Leu Ala Cys Glu 435 440 445

&lt;210&gt; 11 &lt;211&gt; 380 &lt;212&gt; PRT &lt;213&gt; South Arab world Arabidopsis sp. · &lt;400&gt;

Met Arg Ser Arg Pro His Arg Val Val His Asp Leu Ala Ala Ala Ala 15 10 15

Ala Ala Asp Ser Thr Ser Val Ser Ser Gin Asp Tyr Arg Trp Ser Glu 20 25 30 lie Pro Glu Glu Leu Leu Arg Glu lie Leu lie Arg Val Glu Ala Ala 35 40 45

Asp Gly Gly Gly Trp Pro Ser Arg Arg Ser Val Val Ala Cys Ala Gly 50 55 60

Val Cys Arg Gly Trp Arg Leu Leu Met Asn Glu Thr Val Val Val Pro 65 70 75 80

Glu lie Ser Ser Lys Leu Thr Phe Pro lie Ser Leu Lys Gin Pro Gly 85 90 95

Pro Arg Asp Ser Leu Val Gin Cys Phe lie Lys Arg Asn Arg lie Thr 100 105 110

Gin Ser Tyr His Leu Tyr Leu Gly Leu Thr Asn Ser Leu Thr Asp Asp 115 120 125

Gly Lys Phe Leu Leu Ala Ala Cys Lys Leu Lys His Thr Thr Cys Thr 130 135 140

Asp Tyr lie lie Ser Leu Arg Ser Asp Asp Met Ser Arg Arg Ser Gin 145 150 155 160

Ala Tyr Val Gly Lys Val Arg Ser Asn Phe Leu Gly Thr Lys Phe Thr 165 170 175

Val Phe Asp Gly Asn Leu Leu Pro Ser Thr Gly Ala Ala Lys Leu Arg 180 185 190

Lys Ser Arg Ser Tyr Asn Pro Ala Lys Val Ser Ala Lys Val Pro Leu lp5 200 205

Gly Ser Tyr Pro Val Ala His lie Thr Tyr Glu Leu Asn Val Leu Gly 210 215 220

Ser Arg Gly Pro Arg Lys Met Gin Cys Leu Met Asp Thr lie Pro Thr 9l26I.doc • II · 1338695 225 230 235 240

Ser Thr Met Glu Pro Gin Gly Val Ala Ser Glu Pro Ser Glu Phe Pro 245 250 255

Leu Leu Gly Thr Arg Ser Thr Leu Ser Arg Ser Gin Ser Lys Pro Leu 260 265 270

Arg Ser Ser Ser Ser Leu Lys Glu Thr Pro Leu Val Leu Ser Asn 275 280 285

Lys Thr Pro Arg Trp His Glu Gin Leu Arg Cys Trp Cys Leu Asn Phe 290 295 300

His Gly Arg Val Thr Val Ala Ser Val Lys Asn Phe Gin Leu Val Ala 305 310 315 320

Ala Gly Ala Ser Cys Gly Ser Gly Thr Gly Met Ser Pro Glu Arg Gin 325 330 335

Ser Glu Arg lie lie Leu Gin Phe Gly Lys Val Gly Lys Asp Met Phe 340 345 350

Thr Met Asp Tyr Gly Tyr Pro lie Ser Ala Phe Gin Ala Phe Ala lie 355 360 365

Cys Leu Ser Ser Phe Glu Thr Arg lie Ala Cys Glu 370 375 380

&Lt; 210 &gt; 12 &lt; 211 &gt; 1365 &lt; 212 &gt; DNA &lt; 213> Arabidopsis tea Arabidopsissp &lt;. 400 &gt; 12, atgtcgttcc gtagcatagt tcgtgatgtg agagatagta taggaagtct atcgaggcgt 60 agtttcgact ttaagttaag cagcttgaac aaagaaggtg gtaaatcccg tggttcggtt 120 caagattctc atgaggaaca acttgtagta acgattcaag aaacaccgtg ggcgaatcta 180 cctccagagt tattacgtga tgtgatcaaa agacttgaag agagtgaaag tgtgtggcct 240 gctcgtagac atgttgttgc ttgtgcttct gtttgcaggt catggagaga tatgtgtaaa 300 gagattgttc aaagtccgga gctctcaggc aaaatcacat ttcctgtttc gttgaaacag 360 cctggaccaa gagatgcaac aatgcaatgc tttatcaaaa gggataaatc taacttgact 420 tatcatttat atctttgtct cagtcctgct ttgttggttg agaatggaaa gtttcttctt 480 tctgcaaaac gcataagaag aactacatac accgagtacg tgatctctat gcacgccgac 540 accatttcga gatcaagcaa tacctacatt ggcaaaatca ggtctaattt tctggggacg 600 Aagtttataa tatacgatac acaaccagca tacaacagca acatcgctcg agcggtccaa 660 ccggtaggtc ttagccgcag attctactca aagagagtct ctcccaaagt acctagtggg 720 agctacaaaa ttgcgcaggt ttcttatgag ctaaacgttc ttggtacccg tggt ccgagg 780 agaatgcatt gtgcgatgaa ctcaattccc gcctcttccc ttgcggaagg cggaactgtg 840 cctggacagc ccgatatcat tgtcccgcgc tctattctcg acgaatcgtt ccgcagcatt 900 acctcttcgt catcgagaaa aatcacttac gattactcga atgattttag cagtgcacgg 960 ttttccgaca ttcttggccc gttaagcgaa gaccaagaag tggtattaga agaagggaaa 1020 gagcggaatt cgccaccact tgtgcttaag aacaagccgc cgaggtggca tgaacagctt 1080 cagtgttggt gtttaaactt caggggacgt gtaacagtcg catcagttaa gaactttcag 1140 ctcattgcag caaaccaacc acagcctcag cctcagcctc aaccgcaacc tcaaccccta 1200 actcagccgc aaccgtctgg tcagaccgat ggtcccgaca agatcatatt gcagtttggg 1260 aaagtgggaa aagacatgtt cacgatggat ttccggtatc cgctctctgc gtttcaggct 1320 ttcgctatct gtttgagcag tttcgacaca aaacttgctt gcgaa 1365

&lt;210&gt; 13 &lt;211&gt; 1182 &lt;212&gt; DNA &lt;213&gt; Arabidopsis Arabidopsis sp. &lt;400&gt; 13 atgtctttga aaagcatcct tcgtgatctg aaggaagtga gggatggact tggaggcatc 60 9l261.doc 12- 1338695 tccaagagaa gataacatac tggagggttg tcagtatgta gggaagctca tgttttatta tcggagactg acagacttta ggcaaattaa tcatccacag ctacctacga aggggaccta gaaccgtcag gaaaccatca ctagttctca ttcaagggaa gcttctttgg ggtaaggata atatgcatta gctggtcaaa cacagagccc aagagagtga aatcatggag cttttccaat agaggaacag agaacgacaa taatctccct ggtctggttt cacaagccca atagctctac gaagaatgca tagttcaagg caacagacaa aaaacaaatc gagtgactgt atgcgccgcc ttttcaccat gcagctttga gtcgtctcac atgggcttct gacagcttgg aggaatcact ctcattgaaa agcaacagct actgttgtta atctgcaaag tctgggaacc acctaaccga cgtaggaaac ctgcgctatg catagaagag agagattcct cccaagatgg ggcttcagtt tgaagaacat ggattatcgc caccaaaccg attgctcctg Ttgccgcctg cccgctcgag atggagattg cagccggggc acatacattc gcagcaagaa aacttctcac aagttcacaa agactccacc ataacctacg gattctatac gaagtctctt gataattctc catgagcagt aagaatttcc gagagg gtga taccctctat gcatgtgaag atcaaacaac agttgcttca ctgccgttgt tgaggatccc ctcgagactc tctattatgg ggattagaag ggagcagcag tatatgacaa cgaaacaagc agctcaatgt ccctctcttc cctctccttc caagcttaag tgcagtgctg agcttgttgc tcttacagtt ctgcttttca gg tccaccactg tgacattatc ctcttgtgct tgagcagtgt tccaattcaa tttgatgcct agcgacatgc tacttatgtt ccaaacagca ggctcctaaa tcttcgcaca tgttattgct accaaaagga ggaccaaccg gtgcctcaac agagattgac tggcaaaatc agcctttgct 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 1182

gattatcagt accaagatag 14 atgtccttca ggattcgatg tctgttcctg agagatgttc gtttcttgcg cctgagcttt tcacttgttc gggttaaacc cggaggccaa agcaatacct gacgctcagc agtttgaaac tatgagctta atccctgcat agcaatcttg agtctccctt aaagcgccca acagttgctt cctgagcacg; &lt; 210 &gt; 14 &lt; 211 &gt; 1218 &lt; 212 &gt; DNA &lt; 213> Arabidopsis Arabidopsissp &lt;. 400 & gt agagtctcat tcagattcgg ttgatgcttt ttatgaggat ctggtgtctg ctagcaaact aatgctatat aagcagcttc cttgcactga atatcggaaa cgacgaatcc aagtgagccc acgtcttggg cagctgtaga atagtttccc ctggtccatc gatggcacga ccgtcaaaaa aaaacgtgat accctatctc catgtgaa tcaggacatg gtatggtaga caagcagagc tgagcaatcc caggaactgg cacttttcct tatgagaaac aaatgatgat ctacatcatc gcttagatct tggaactcag gagaattcca ttccagagga acctggagga ctcattctcc atctgctgct acagctccag ctttcagctg tctccagttt tgccttccag agaggagagc tccaggtctc tgctgggcta gaagacactt cgagaaatcg atctccctca cgcagcaatc ggaaagttcc tccttaaact Aactttctgg gttaccagaa tctggcaact ccgaggagga acagctccaa ttcttcaggt cagaaggaag tgctggtgcc gtagctgctc ggaaaagtcg gccttcacca tt gggagtat aacgtgttgt gtatgcctcc ggccgtctag tcaaagagat aacagccggg aaacctacta ttcttgctgc gcgatgatgt ggaccaagtt cccgttcaag atcctgtagc tgcagtgtgt ctcagacgga cgaaatcaat gactgcttgt tcaacttcaa ctgagaatgg gaaaagatgt tttgcctcag atccagaaag tcaggatact ggagctcctg gaaaaatgtt cgtcagagtt tcctagagga tctatacctc caagaggttt ctctcgagga cactgtctat cagacttctc acatatctca catggatgcc acttgtccat tcgtgcagag cctgaaaaac tgggagagtc acctgcagga gttcacaatg cagtttcgac 000000000000000000008 628406284062840628401 11233445667789900122 11111

&Lt; 210 &gt; 15 &lt; 211 &gt; 795 &lt; 212 &gt; DNA &lt; 213 &gt; Arabidopsis tea Arabidopsissp &lt;. 400 &gt; 15 atgcctcctg agcttctgag agatgttctg atgaggatag agcgatccga agacacttgg 60 ccttctagga agaatgttgt ttcttgtgta ggtgtgtgta agaactggcg acaaatattc 120 aaagagatcg ttaacgttcc tgaggtttct agcaaattca cttttccaat ctccttgaaa 180 91261.doc 13- 1338695 cagcctggtc caggaggatc acttgttcaa tgctatgtta agagaaaccg tagcaatcaa 240 actttctatc tataccttgg aggtgaagca aaaatatttt gtcagtctga accaagtgat 300 atttatctcg ttccttacag ttacagagag acgcattgcg tcatggatgc catctctgca 360 tcagcagtaa aacctggagg aacagctaca actcagacag aactcgataa tttcgtgtca 420 ttcaggtctc cttctggtca aaaggaagga gtgcttgttc ttaagagcaa agtgcctaga 480 ttggaagaac agagctggtg tctcgacttc aatggctctc ctgagaacga acctgagaat 540 gaaaacgaca ttttccagtt Tgcgaaagtc ggaaacttgc acaaactctt cagtttatat 600 gaggctgaat ggattcctct cgttcgcacc tcagtgtttg ctgtcattgc tcgagtttgt 660 agagataaaa agcatacacc atcgtatgaa ttgaaacttg cattgtactt tgcaaaaaac 720 tctgcaatcc tcaagaaatt cgttctccgc ggttaca Ctc gagaagaaga tttactcgca 780 ttgcccgtgg ctaac 795 &lt;210&gt; 16

&lt;211&gt; 12Θ7 i*· &lt;212&gt; DNA &lt; 213 &gt; Chardonnay Arabidopsis sp. &lt;400&gt; 16

atgtcgtttc tgagtattgt tcgtgatgtt agagatactg taggaagctt ttcgagacgt 60 agtttcgacg tgagagtatc taatgggacg actcatcaga ggagtaaatc tcacggtgtt 120 gaggcacata ttgaagatct tattgtaatc aagaacactc gttgggctaa tttaccggct 180 gcgctattac gagatgtgat gaaaaagttg gatgaaagcg agagtacttg gcctgcacgt 240 aaacaagtcg ttgcttgtgc tggtgtctgc aagacatgga gactaatgtg caaagatatt 300 gtgaaaagtc ctgagttctc aggcaaactc acatttccag tttcgttgaa acagcccggg 360 cctagggatg gaatcataca atgttatatc aaaagagaca agtctaacat gacttaccac 420 ctttaccttt ctcttagtcc tgccatactt gttgaaagtg ggaagtttct tctctcggca 480 aagcgctcac ggagagctac atacacagag tatgtaatat caatggatgc agacaacatt 540 tcaagatcaa gcagcactta cattggcaaa ctgaagtcta actttctagg gacaaaattt 600 atagtatatg atacggctcc tgcgtacaac agtagccaga tattgtcccc accaaaccgg 660 agtcgtagtt tcaactccaa gaaagtgtct cccaaagtcc cttctggaag ttacaacatt 720 gctcaagtta catacgagct gaacttgctt ggaacccgtg gacctcgtag aatgaactgc 780 attatgcact ctatcccctc cttagctcta gaacccggag gtactgtccc tagccaacct 840 gagtttctac aacgttccct tgatgaatct ttccgcagca tcggttcctc aaagatagtc 900 aaccactcgg gagatttcac ccgaccgaaa gaggaagaag gaaaggtgcg acctttggta 960 ctgaaaacta aaccgccaag gtggctccaa ccgttgcgat gttggtgcct taacttcaaa 1020 gggagagtga ctgtagcttc tgtcaagaac ttccagttga tgtccgctgc aacggttcag 1080 cccggtagtg gtagtgatgg tggagcattg gctacgaggc catcgttatc accacagcag 1140 ccagagcaat caaaccatga taagataata ctacactttg ggaaagtggg taaggatatg 1200 ttcactatgg actatcgtta tcctctctct gcctttcaag cgtttgccat ttccctgagc 1260 acctttgata ctaaattggc atgtgaa 1287

&lt;210&gt; 17 &lt;211&gt; 1164 &lt;212&gt; DNA &lt; 213 &gt; 213 South Korea Arabidopsis sp. &lt;400&gt; 17 60 120 180 240 300 360 420 480 540 600 atgtcattga agaacatagt gaagaacaaa tacaaagcta ttggtagaag agggaggtca cacattgcac cagaaggatc atctgtgtct tcttctttat caactaatga aggtttaaac cagagtattt gggttgattt gcctccagag ttacttcttg atataatcca aaggattgag tctgaacaga gtttatggcc ggggaggaga gatgttgttg cttgtgcttc ggtttgtaag tcatggaggg agatgactaa agaagttgtt aaagttcctg agctctctgg tttgatcacg tttccgattt ctttaagaca gcctggacct agagatgctc caattcaatg ctttattaaa cgtgaaagag ctacggggat ataccgtctc tatcttggtt taagccctgc tctttccggt gacaagagta agttgttgtt atctgcaaag agagtcagga gagcgacggg tgcggagttt gttgtatcgt tatcggggaa tgacttctcg agaagtagta gtaattacat aggaaaactg agatcaaatt tcctgggaac gaagttcaca gtctacgaaa accaacctcc tccgtttaac 91261 .doc 14- 1338695 cgaaagctcc caccatcgat gcaagtgtct ccatgggtat cgtcgtcatc tagtagttac aacatagctt caatcttgta tgagctgaat gttctgagaa ccagaggtcc aagaagaatg caatgtataa tgcacagtat cccgatttca gcgattcaag aaggc ggcaa aatccagtcg ccaacggagt tcacaaacca aggaaagaag aagaagaagc cgctgatgga tttctgctca gggaacctgg gaggagaatc cgttataaaa gaaccattaa ttctgaaaaa caagtcgccg agatggcacg aacagcttca gtgctggtgt ctaaacttca aaggtcgagt cacagtcgcc tcggtgaaaa acttccagct agtggcagct gctgcagaag cagggaagaa catgaacata ccagaagagg aacaagatag agtgatatta cagtttggga agataggcaa agacattttc acaatggatt atcgttaccc gatctctgca ttccaagctt ttgctatttg tttaagcagc ttcgacacga agccagtctg cqaa

gctggactct aaggatggtt 18 atgcctttgt ggagagacaa tcttcgtcat gagactgagg aaatggagag ttcccttctt aggaacaaga aagggaaagt atatcacttg tcagattttc aaaccttcaa ccagcaggca ggtccaagaa; &lt; 210 &gt; 18 &lt; 211 &gt; 1137 &lt; 212 &gt; DNA &lt; 213> Arabidopsis Arabidopsissp 0000000004 6284062846 6 7 78990011 1111 &lt;. 400 & gt cagtgctggt ctggttgcga ctacagtttg gcatttcagg cacggtccct cgacggcacc ggtcggcgat accgttggcc aaatcactca gcctcaaatt agacatcaac ttcttctggc atgctgatga ttgggaccaa atggcaaagc actttgaagt gaatggtaag cgtctgacca ccagcttgac gtctgaactt ccgttgacca gtaaagtggg cttttgctat cctttcgcgg ggaatccgaa gctccctgaa tcaacgtcgt cgatttcgct gccagcftcct gttttacttg ggcgcggagg tttctctcaa ctttacagta cagtcgcaga cggtcatgtt cacactccga aaagccatgt aatactaaag ccatggacga aagtcaaccg agatgacact ctgtctcaca aggatctcga tcgattcctc ttattaggcg gatgtagtta agatcctctc agagactttt tatcttgctc tttaggaccg Ggaagtaatg tacgatagcc tttgcatcaa tcttataaat tgcccatcac gatgtaacca aacaaagctc gttactgttg agcggtaaag ttcactatgg agtttcggca actcttttag cgccgtcgaa aga tcattcg cttgcgcttg ttaactctgg ctaatcagtg taacaccatc gtgcttacac cctacgtcgg aaccaccaca agcagataag tcaacctttt catcaccttc agataatgaa ctagatggca cttcggtcaa gcgatgaaga attatagaca ctaaacttgc gtttcateag tatggccggt tcgcgtggag cgtttctaag caaaattact cttgataaag attcactgat tgagtacatc aaaattaaga caacggagca ccctcaagtt gaaatcaaga atcatcatcc aaaacccaac cgagcacttg gaactttcag aacagttctt gcctctctct ctgcgag 60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1137

&Lt; 210 &gt; 19 &lt; 211 &gt; 1191 &lt; 212 &gt; DNA &lt; 213 &gt; Quasi Nanfen Arabidopsis sp &lt;. 400 &gt; 19 atggctggtt attacagggt gtctctacct ggtaactctg acagctctga agtagtttcg gtgtgggagt agggctttgt aaagaacaat ggtcgggggc tctatattta gtcggttcca cgagttggtt accataacaa cgagaaaagt ctttatccac ctgtggaaac ggtttccaac agcagtcatc gaatgaaaac tttcggattc tgtgcaagac cacctgaagg gtaaagaccg gggtggcaca tgacggctaa ctgtaggtaa cttggacagg gaatgatttg tcaaaaggga tcggaaacta ggaagttaca actgcaagcg ttggactagt tgaagctgcc actacctttg cttgagccac gaagttagca gaatgttaag tctcttgggt aatggtgaag gagctacaga ttggaggaaa gaggataagg gatcgagctt aatttcaaat tgtatgcaga gttgattcag cctgcttcct gatgtgggaa ggatctgtat gttgcttacc ggattgctgt tccaagtact ccgcttcttt agaatgagaa ataagggaaa agaatgtgtc tgaagtctca ctttctcaac agaacagtga agcattcaag cttggtctac gatgcacttg attcacttta atagccgacg gcagttcgga acatatggga cggttgtaat Ctttgctacc tgtggacaca gccggagaaa atcgatgaag tggtggtcga ggttgataag ttcgttgaaa tttgcccaac tctgattgtg tacacatgaa taatgggaaa tgaaagttat caaggg agtt attcacaccc aaagcagcag 60 120 180 240 300 360 420 480 540 600 660 720 780 840 9126l.doc] 5- 1338695 ccaatgcaga aaaacaacaa cttgagaata aggaaaaaat tccaagcagc atgagttaga tcggtgaaga actttcagct gggagagttg acaaagcaag gcattctgca tttgcttggc taagcaggtt tcagaagcta cttcagagac aacactcacg atatgtaatc ttctattgat caacaagcta tgctcaagga agaggaagaa gagactccaa gacttcaggt tccaagcttt gtaaactacc taccacatta Caggactgag ggcagacaat atccattctc gttgtactgt gcttgattgg caacaaaatc aatacagagc tcttcaaatg aggctaccaa 900 960 1020 1080 1140 1191

&Lt; 210 &gt; 20 &lt; 211 &gt; 1140 &lt; 212 &gt; DNA &lt; 213 &gt; Quasi Nanfen Arabidopsissp &lt;. 400 &gt; 20 atgacgttcc gaagtttact ccaggaaatg cggtctaggc cacaccgtgt agttcacgcc gccgcctcaa ccgctaatag ttcagaccct ttcagctggt cggagctccc ggaggagctg cttagagaaa tcctgattag ggttgagact gttgacggcg gcgattggcc gtcgcggcga aacgtggtgg cttgtgccgg cgtttgtcgt agctggagga ttctcaccaa ggagattgta gctgttcctg aattctcctc taaattgact ttccctatct ccctcaagca gtctggtcca agagattctc tagttcaatg ctttataaaa cgtaatcgaa atactcaatc gtatcatctc tatctcggat taactacctc tttgacggat aacgggaagt ttcttcttgc tgcttctaag ctgaagcgcg caacttgcac tgattacatc atctctttgc gttcagacga tatctcaaag agaagcaacg cgtatcttgg gagaatgaga tcgaacttcc ttggaacaaa attcacggtc tttgatggta gtcagaccgg agcagcgaag atgcagaaga gccgctcttc taatttcatc aaagtttcac ctagagttcc tcagggaagt taccccatcg ctcacatttc atacgagtta aacgtcttag gctctcgggg accgagaaga atgcgttgca tcatggatac Aatacctatg agcatcgtgg agtcgcgagg agtagtagct tcaacatcca taagctcttt ttccagtcgg tcatcaccag tctttaggtc tcactcaaaa ccattgcgc a gtaatagtgc atcatgtagc gactcaggca acaacctggg agatccacca ttggtgctga gcaacaaagc tccacggtgg catgagcagt tacgttgctg gtgcttaaat ttccatggtc gagtcacagt ggcttcggtt aagaactttc agcttgtggc agttagtgac tgtgaagcag ggcagacatc tgagaggatc atactccagt ttgggaaagt tgggaaggac atgtttacca tggattatgg atatccgatt tctgcgtttc aagcgtttgc tatctgcctg agcagttttg aaaccagaat tgcctgtgaa

&lt;210&gt; 21 &lt;211&gt; 1335 &lt;212&gt; DNA &lt;213&gt; Arabidopsis Arabidopsis sp. 60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140

&Lt; _ &gt; 21 atgtcgtttc agtttcgatt gagtattcgt aatcttcctc tggcctgcaa tgccaagaga aaacagccag ctaacatttc cttctttcag gctgataaca gggacaaagt atcactgatc tccggaagct ccacgacgaa tcagtcccta cgcagtaaca tctagattct ccgttgattc gaggcattgt ttaggctctc catcccgtga cagagttact gaaaacatgt ttgttttggg ggcctcgtga acctttttct ctaaaagaac tctcaagatc tcttggtgta gaacaagccg acaacattgc tgcactgcat accaacccga tctccttctc ccgaaatggg taaagaacaa tcaagatttg gagtcttcat tctcttgtcg ctttgatgtg tgtggcttgt gcctgaaatc tgcaatgatt ttgtttaagt tcgtagaact cagcaactct cgacacgcaa aagcaggttt tcaaatcacc catgaactcc gaaactcgtc caaatcatca aatatcctgc gcagccaagg agagatgggt aaagggaaag cctgtgatag atcaaaagat gcttcggttt tgtgggaaac cagtgtttca cccgctctat actcgaaccg tacctcggaa ccaccaccaa cactccagac tatgagctca atcccaattt cctgcaccat tttgaccacc gacgacaacg tggcacgagc ctcagggttc ttcagacaag tagaggaaag gtcggtcttg tcactttccc tcaaaaggga tagtggagaa agtacattat agctcagatc acacatcttc gagtttctcc acgtgttggg catcgctcga actctctcga gctccctcga aagaagaagc agttgcaa ttgggagctt Tg gtcaaggagg ttcgttccgt tagatgggct tgagagtaat gagagctatg tgtttccctc taaatcaaag tgggaaattt ctccatggat aaacttcctt gagcgcactt taaagtacca cacacgcggg accaggcggt cgactcattc tttcagcagt gagtttcaga ctggtgtttg 60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080

91261.doc 16- 1338695 aatttccgcg gacgtgtgac agttgcatcg gttaagaatt tccagcttgt agcagcaaga cagccgcagc ctcaagggac aggtgcagca gcagcaccaa caagtgcacc tgctcaccct gagcaagaca aggtgattct ccagtttggt aaagtaggga aagatatgtt cacaatggac tataggtatc cattatcggc gtttcaggcg tttgcgatat gcttaagcag ctttgacacc aagcttgctt gtgaa

&lt;210> 22 &lt;211&gt; 1140 &lt;212&gt; DNA &lt;213&gt; 南南齐Arabidopsissp. 0 0 0 0 5 4 0 6 2 3 12 2 3 3 11111 &lt;400&gt; 22 atgcgttcga acttctgtgt attctgattc gcttgtgccg gagatctctt ctggttcaat ttaaccaact acaacttgta gcttatgttg aatctgctgc aaagtttcag aatgtcttag agcacaatgg cggtcaacct gaaacaccat tgcttgaatt gcaggagcta atattgcagt tcagctttcc gaccgcatcg catcgcaaga gtgttgaagc gcgtttgtcg ctaagttgac gctttatcaa ctttaacgga cggattacat gcaaagtgag cttcaacggg caaaagttcc gatcccgggg agcctcaagg tatccaggtc tagtgctgag tccatggccg gctgtggcag ttgggaaagt aggcttttgc tgtggtccac ttatcgctgg ggcggacggt tggctggcgg tttccccatc acgtaatcga tgatgggaag tatctcttta atcgaacttc agccgcaaag tcttggaagt accaagaaag agtagcttca tcagtcaaaa caacaagaca tgtcacagta tggcacggga cgggaaagat catttgcttg Gaccttgccg tcagagattc ggcggatggc ctacttatga tctctcaagc attacgcaat tttttgcttg cgttctgatg ctaggaacga ttgagaaaga tatcctgtcg atgcaatgtc gaaccatcag ccattacgca ccacggtggc gcgtcagtga atgtcaccgg atgttcacga agcagctttg ccgccgcagc ctgaagagct cgtcacga cg acgaaaccgt agcctggtcc catatcatct ctgcgtgtaa atatgtcgag aattcactgt gccgatctta ctcatatcac ttatggacac agtttccctt gtagctcaag acgagcagct agaactttca agaggcagag tggattatgg agactagaat tgccgattcc tcttagggag cagcgtggtg cgttgtccct ctatctcgga gttgaagcac aagaagccaa ctttgatgga taatcccgca atatgagctg aatacctaca actcggtact ccacctgaaa acgctgctgg gctcgtggca cgagcggatt atacccgatc cgcttgtgaa 60 120 180 240 300 360 420 480 540 600 660 720 780 840 900 960 1020 1080 1140 &lt;210&gt; 23 &lt;211&gt; 24 &lt;212&gt; DNA &lt;213&gt; artificial sequence &lt;220&gt;&lt;223&gt; primer &lt;400&gt; 23 24 atgtcgttcc gtagcatagt tcgt &lt;210&gt; 24 &lt;211&gt; 25 &lt;212&gt; DNA &lt;213&gt; artificial sequence &lt;220&gt;&lt;223&gt; primer &lt;400&gt; 24 25 ttattcgcaa gcaagttttg tgtcg

&lt;210&gt; 25 &lt;211&gt; 28 &lt;212&gt; DNA 9126 丨.doc 1Ί- 281338695 &lt;213&gt; artificial sequence &lt;220&gt;&lt;223&gt; primer &lt;400&gt; 25 atgtctttga aaagcatcct tcgtgatc &lt;210&gt;&lt;211&gt; 28 &lt;212&gt; DNA &lt;213&gt; artificial sequence &lt;220&gt;&lt;223&gt; primer &lt;400&gt; 26 ttacccttca catgccggtt tggtgtca &lt;210&gt; 27 &lt;211&gt; 24 &lt;212&gt; DNA &lt;213&gt; artificial sequence 28 &lt;220&gt;&lt;223&gt; primer &lt;400&gt; 27 atgtccttca agagtctcat tcag &lt;210&gt; 28 &lt;211&gt; 24 &lt;212&gt; DNA &lt;213&gt; artificial sequence 24 &lt;220&gt;223&gt;Introduction&lt;400&gt; 28 tcattcacat gctatcttgg tgtc &lt;210&gt; 29 &lt;211&gt; 23 &lt;212&gt; DNA &lt;213&gt; Artificial Sequence &lt;220&gt;, &lt;223&gt;Introduction&lt;400&gt; 29 atgtcgtttc tgagtattgt teg &lt;210&gt; 30 &lt;211&gt; 23 &lt;212&gt; DNA &lt;213&gt; Artificial sequence 24 23 91261.doc 18-231338695 &lt;220&gt;&lt;223&gt;Introduction&lt;400&gt; 30 ttattcacat gccaatttag tat &lt;210&gt; 31 &lt;211&gt; 23 &lt;212&gt; DNA &lt;213&gt; artificial order &lt;220&gt;&lt;223&gt;Introduction&lt;400&gt; 31 atgtcattga agaacatagt gaa &lt;210&gt; 32 &lt;2U&gt; 23 &lt;212&gt; DNA &lt;213&gt; Artificial sequence 23 &lt;220&gt;&lt;223&gt;Introduction&lt;400&gt; 32 tcattcgcag actggcttcg tgt &lt;210&gt; 33 &lt;211&gt; 21 &lt;212&gt; DNA &lt;213&gt; artificial sequence 23 &lt;220&gt;&lt;223&gt; bow &子&lt;400&gt; 33 atgcctttgt cacggtccct c &lt;210&gt 34 &lt;211&gt; 22 &lt;212&gt; DNA &lt;213&gt; artificial sequence 21 &lt;220&gt;&lt;223&gt; primer &lt;400&gt; 34 tcactcgcag gcaagtttag tg &lt;210&gt; 35 &lt;211&gt; 23 &lt;212&gt; DNA &lt;213&gt; artificial sequence 22 &lt;22〇&gt;&lt;223&gt; primer 91061.doc 19-231338695 &lt;400&gt; 35 atggctggtt cgagaaaagt gaa &lt;210&gt; 36 &lt;211&gt; 23 &lt;212&gt; DNA &lt; 213 &gt; artificial sequence &lt;220&gt;&lt;223&gt; primer &lt;400&gt; 36 tcaaacagta caacaaagct tgg &lt;210&gt; 37 &lt;211&gt; 23 &lt;212&gt; DMA &lt;213&gt; artificial sequence 23 &lt;220&gt;&lt;223&gt;;introduction&lt;400&gt; 37 atgacgttcc gaagtttact cca &lt;210&gt; 38 &lt;211&gt; 23 &lt;212&gt; DNA &lt;213&gt; artificial sequence 23 &lt;220&gt;&lt;223&gt; primer &lt;400&gt; 38 ttattcacag gcaattctgg ttt &lt;210&gt; 39 &lt;211&gt; 23 &lt;212&gt; DNA &lt;213&gt; artificial sequence 23 &lt;220&gt;;&lt;223&gt;Introduction&lt;400&gt; 39 atgtcgtttc gaggcattgt tea &lt;210&gt; 40 &lt;211&gt; 23 &lt;212&gt; DNA &lt;213&gt; Artificial sequence 23 &lt;220&gt;&lt;223&gt;Introduction&lt;400&gt; 40 91261.doc 20- 1338695 ctattcacaa gcaagcttgg tgt &lt;210&gt; 41 &lt;211&gt; 23 &lt;212&gt; DNA &lt;213&gt; artificial sequence 23 &lt;220&gt;&lt;223&gt;Introduction&lt;_&gt; 41 atgtcgtttc tgagtattgt teg &lt;210&gt;42&lt;211&gt; 23 &lt;212&gt; DNA &lt;213&gt; artificial sequence 23 &lt;220&gt;&lt;223&gt; primer &lt;400&gt; 42 ttattcacat gccaatttag tat &lt;210&gt; 43 &lt;211&gt; 23 &lt;212&gt DNA &lt;213> artificial sequence 23 &lt;220&gt;&lt;223&gt; primer &lt;400&gt; 43 atttctcaaa atcttaaaaa ett &lt;210&gt; 44 &lt;211&gt; 20 &lt;212&gt; DNA &lt;213&gt; artificial sequence 23 &lt;220&gt;&lt;223&gt;Introduction&lt;400&gt; 44 tgatagtttt cccagtcaac &lt;210&gt; 45 . Lt;211&gt; 21 &lt;212&gt; DNA &lt;213&gt; artificial sequence &lt;220&gt;&lt;223&gt; primer &lt;400&gt; 45 atgacgttcc gaagtttact c 20 21 9J261.doc -21 - 211338695 &lt;210&gt; 46 &lt;211&gt 21 &lt;212&gt; DNA &lt;213>artificial sequence&lt;220&gt;&lt;223&gt;primer&lt;400&gt; 46 tggttcacgt agtgggccat c &lt;210&gt; 47 &lt;211&gt; 21 &lt;212&gt; DNA &lt;213&gt; Sequence &lt;220&gt;&lt;223&gt;Introduction&lt;400&gt; 47 21 ttattcacag gcaattctgg t &lt;210&gt; 48 &lt;211&gt; 21 &lt;212&gt; DNA &lt;213&gt; Artificial Sequence &lt;220&gt;&lt;223&gt;;400&gt; 48 21 tggttcacgt agtgggccat c 9l261.doc 22-

Claims (1)

1338695 _ ~~ * -—— ♦ 曰 曰 repair (more) original patent No. 093118382 - ~ Chinese patent application scope for #99^12: ^ X. Patent application form - 1. An isolated polypeptide, It comprises a sequence having at least 9% identity with seqidn(R): 12 3, 4' 5 6 7 8 , 9 , 1 , or II, the ten of which has the ability to modulate the response of Arabidopsis to environmental factors. 2. According to the scope of the patent application! An enzyme, wherein the sequence has at least 95 〇/〇 identity with SEQ ID ΝΟ··1, 2, 3'4, 5, 6, 7 〇, 8, 9, 10 or 11, wherein the polypeptide has a steel shovel + The ability to modulate Nansuke's response to environmental factors. 3. Polymorphic NOs: 1, 2, 3, 4, 5, 6, 7, 8, ′ where the sequence is SEQ ID 9, 10 or 11 according to the scope of claim 2 of the patent application. 4. An isolated nucleic acid comprising the sequence of a polypeptide according to any one of claims 1 to 3 of the present invention. A knife-extracted nucleus which hybridizes under stringent conditions to a sequence selected from the group consisting of HD N0s: 12_22 or a complete complement thereof, and encodes the A t T L P protein of the pseudo-Southern. 6. A vector comprising a nucleic acid according to item 4 of the scope of the patent application. A vector comprising the nucleic acid according to the scope of claim 5 of the patent application. 8. A host cell comprising a nucleic acid according to item 4 of the scope of the patent application. 9_ A host cell comprising a nucleic acid according to item 5 of the patent application. The host cell according to the eighth aspect of the invention, wherein the host cell is an Escherichia coli, yeast, insect, plant or mammalian cell. The host cell according to claim 9, wherein the host cell is an Escherichia coli, yeast, insect, plant or mammalian cell. 12. A method of making a polypeptide comprising growing a host cell in a medium which can express the polypeptide according to the patent application scope 91261-99l227.doc 1338695, and detaching the polypeptide. 13. A method of making a polypeptide, the method comprising growing from a host cell which can express the polypeptide according to the U through the scope of the patent application, and detaching the polypeptide. A transgenic plant cell lacking a polypeptide comprising a sequence of seQ IDNO:l'2, 3, 4, 5, 6, 7, 8, 9, 10 or 11 when compared to a wild-type cell The polypeptide is not expressed and is therefore highly resistant to salt, low temperature, pathogenic bacteria, oxidative stress or water shortage. 15. The transformed plant cell according to claim 14, wherein the cell is an Arabidopsis cell. 16. A method of making a transformed plant cell, the method comprising introducing into a plant cell a polypeptide encoding a SEQ ID 1、: 1, 2, 3, 4, 5, ό, 7, 8, 9, 10 or 11 A gene-expressing nucleic acid in which the transformed plant cell is highly resistant to salt, low temperature, pathogenic bacteria, oxidative stress or water shortage due to the lack of the polypeptide. 17. A method of making a transgenic plant, the method comprising introducing into a plant cell a polypeptide encoding a SEq ID: 1, 2, 3, 4' 5, 6, 7, 8, 9, 10 or 11 The gene-expressing nucleic acid' and the cultured cell-forming plant, wherein the transgenic plant is more resistant to salt, low temperature, pathogenic bacteria, oxidative stress or water shortage due to the lack of the polypeptide when compared with the wild-type plant. 18. A transformed plant cell comprising a recombinant nucleic acid encoding a SEQ ID NO: 3, *, 5'6, 7, 8, 9, (7) or a scorpion heterologous polypeptide. 91261-991227.doc -2 - 19.1338695 A method of making a transformed plant cell, the method comprising: introducing a SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, in a plant cell 20. A recombinant nucleic acid of 10 or 11 heterologous polypeptides' and the polypeptide is expressed in the cell. A method for producing a transgenic plant, the method comprising: introducing into a plant cell a recombinant encoding a SEQ ID ΝΟ: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or 11 heterogeneous polymorphism The nucleic acid 'expresses the polypeptide in the cell, and the cell is cultured to form a plant. 9126l-991227.doc