TWI329032B - Method for fabricating aerogels and their applications in biocomposites - Google Patents

Description

IX. Description of the Invention: [Technical Field] The present invention relates to a method for producing an aerogel, and more particularly to an aerogel manufacturing method using an ionic melt and its biological application. [Prior Art] Aerogel is an extremely light material, and its density can be as low as 0.003 g/cm3, so there is an alias for frozen smoke. Aerogels are also a very fine solid with a skeleton 'because its porosity can be as high as 99.9%, and the solid is filled with air' and is called Aerogel. Due to the extremely small size of the aerogel structure, it is in a transparent state in most cases. The aerogel has a network structure and a very high specific surface area, which can support 1600 times its own weight. The only drawback is that the structure is very fragile. Almost all aerogel applications are associated with their mesh structure above 9〇% porosity. Due to their low density, low thermal conductivity, bass transmission speed, and low dielectric constant, aerogels become Excellent thermal, acoustic, color, and '彖 materials. From heat insulation materials, sound insulation materials, electrical insulation materials, adsorption materials, catalyst or catalyst carriers, filter materials, dust collection materials, gamma (3) to capacitor materials, it can be used in a wide range of applications. As a drug controlled release carrier. 1329032 1 1 * , In general, metal oxide aerogel catalysts mostly use metal alkoxides (metalalkoxides) as precursors to synthesize wet gels (wetgel) using sol-gel (10) technology. It is prepared by extracting and drying a critical fluid. However, the miscellaneous flow _ temperature ship force strip is very riding, the activity of the coated money molecule or the extraction of the coated material is removed, and the biocomposite (or bio-cladding material) is unfavorable. Production. Furthermore, the supercritical fluid processing procedure is cumbersome, and there is an possibility of explosion during operation, which is dangerous. In view of this, it is still necessary to develop new aerogel manufacturing methods to meet the needs of the industry. SUMMARY OF THE INVENTION In the context of the above-described invention, in order to overcome the disadvantages of the conventional art, the present invention provides a method of manufacturing a seed and its biological application.

The object of the present invention is to adjust the pore size and the ratio of the turbidity by adjusting the amount of the ion immersion liquid having the same nature and controlling the amount of the turbid liquid. Because the ion ^ has the characteristics of no volatility, non-flammability, easy preparation, and easy separation and re-use, it can meet the world trend of environmental protection. Another object of the present invention is to provide a surface-modified gas-to-air process for use in the separation of the mechanical/woven composite (4) and the gas-line composite material for adsorption, catalysis, separation, and drug release materials. Accordingly, the present invention can meet the economic benefits and industrial applicability of the 7 v 1329032 *. In accordance with the above objects, the present invention discloses a method for producing an aerogel, a method for producing a surface-modified aerogel, and a method for producing a biocomposite. Taking the biocomposite coating method as an example, firstly provide a precursor solution, 'pure-containing hydrophilic ion, a generous hydrolysis and/or condensation reagent, at least one biomolecule and at least one oxygen monomer (10)峨 / or Fang Lu green based monomer coffee). It is: owing, a curing process is performed on the precursor solution to facilitate at least -, or (4) oxygen filament riding a hydrolysis reaction and polymerization, thereby curing and coating at least one biomolecule to form a biocomposite. The composite is then subjected to an extraction procedure by a solvent to replace the ion impurities contained in the composite. Next, a drying process is performed on the composite transfer to remove the layup contained in the composite to form the above-described biocomposite. [Embodiment] The direction of the present invention as discussed herein is an aerogel manufacturing method and a biological application. In order to thoroughly understand the present invention, the following pointers will be clarified and thin. _ ground, the hair loss line = the skill of the field is familiar _ special details. On the other hand, it is well known that the ., and the steps or the county are described in the fine materials, and the employment is responsible for the unnecessary restrictions. The present invention will be described in detail below, but the present invention can be widely applied to other embodiments in addition to the details of <s 8 1329032 v, * and the scope of the present invention is not Limited, which is subject to the scope of the patents that follow. A first embodiment of the present invention discloses a method for producing an aerogel, which first provides an aerogel precursor solution comprising an ionic melt, a catalytic hydrolysis and/or condensation reaction reagent, and at least one alkoxy monomer ( Alk〇xide) and/or aryloxide, wherein the ionic solution has both a template and a solvent function. The central element in the above alkoxy monomer and/or aromatic oxy monomer comprises one of the following groups: Li, Na, K, Rb, Cs, Be, Mg,

Ca, Sr, Ba, Ti, Te, Cr, Cu, Er, Fe, Ta, V, Zn, Zr, A Bu

Si, Ge, Sn, and Pb. Common alkoxy monomers and/or aromatic oxy monomers comprise one of the following groups: tetramethyl 〇rth〇silicate (TM0S), tetraethyl orthosilicate (TEOS), tetrabutyl titanate (TBOT) and vanadium oxytnpropoxide. The above catalytic hydrolysis and/or condensation reaction reagent comprises one of the following groups or any combination thereof: an alcohol (carbon is less than or equal to 5), an acidic compound and a basic compound, thereby catalyzing the alkoxy monomer and / Or hydrolysis and polymerization of aromatic oxy monomers. Further, another method of forming aerogel precursor solution comprises: mixing an alkoxy monomer and/or an aryloxy monomer and an ionic melt to form a first mixture. Next, an acidic compound is added to the first mixture to form a first mixture. Finally, an assay compound is added to the second mixture to effect hydrolysis and polymerization of the south alkoxy monomer and/or the aromatic oxy monomer. Then, perform a uniform mixing procedure on the aerogel precursor solution (Example 9 such as strong agitation or strong shock) to disperse the components of the precursor solution as much as possible, especially the ionic melt (because the ionic melt has a template) The function of fully dispersing the template can increase the pore volume of the final aerogel, and fully hydrolyze and condense the alkoxy monomer and/or the aromatic oxy monomer. The above uniform mixing procedure is continued to the aerogel precursor solution. A specific viscosity is achieved, and the specific viscosity is greater than or equal to 150 cps, preferably greater than or equal to 2 〇〇 cps. After completion of the homomixing process, the aerogel precursor solution is allowed to stand to facilitate continuous hydrolysis and condensation of the alkoxy monomer and/or the aryloxy monomer, thereby forming an aerogel. The uniform mixing procedure described above is particularly helpful in improving the porosity and mechanical properties of the final aerogel product. Taking the dioxide dioxide aerogel as an example, referring to the first figure, the final sample is ground and then subjected to solid state nuclear magnetic resonance. Spectrum (Edition _ 29〇·

Si-NMR analysis, Table 1 shows the relative position and structure of the telluride in __295ί_ΝΜκ, and two absorptions + can be observed in the first figure, respectively, at 5=_1〇2 and -107~-i10ppm, where m11 The absorption peak of 〇ppm is the main absorption peak, and its phase structure is Q4, and the intensity is much larger than the absorption peak of (4), and its structure is Q; When the silk-based monomer is Cong, the form of Q2, Q3, and Q4 will be formed according to the degree of hydrolysis. When the positions of Q2 and Q3 are condensed, it will be converted into the form, and the peak intensities of Q2 and Q3 are relative. Attenuated 'and the peak intensity of Q4 is relatively enhanced, so the more Q4 structure, indicating that the siloxane turns completely and the canal cross-linking is better, as shown in the figure. Table I

Structure OR HO-Si-OR OR HO-Si-OR OR RO-Si-OR OH OR OR Peak Q2 Q3 Q4 δ (ppm) -91 -101 -109 丄329032 When the above-mentioned catalytic hydrolysis and/or condensation reaction reagent contains In the case of alcohols, the homogeneous mixing procedure described above may comprise a heating sequence having a temperature in the range of from about room temperature to about 7 °C. The above-mentioned common components for forming an aerogel include one of the following groups or a combination thereof _Si〇2, Ti〇2, V2O5 and Al2〇3, and the aerogel has an average pore diameter ranging from about 1 nm to 50 nm. The porosity ranges from 50% to 99%, and the pore volume range is greater than or equal to 1.0 cm3/g. In addition, the specific surface area varies with the type of aerogel. For example, the specific surface area of the Si〇2 aerogel is greater than or equal to 500 m / g. The specific surface area of the V2〇5 aerogel is greater than or equal to 100 m2/g. The specific surface area of Ti〇2 and Al2〇3 aerogel is greater than or equal to 200. Further, after the aerogel is formed, an extraction process may be performed by a solvent to replace the ionic melt contained in the pores of the aerogel. Next, a drying process is performed to remove the solvent contained in the aerogel aperture. The above solution is preferably selected as a low boiling point solvent (having a boiling point of less than or equal to 2 Å. 〇, comprising one of the following groups: nitrile, _, _ 贞 and water. Operating temperature of the above extraction procedure Fan _ is paper to 2 〇 (TC. Because the method of manufacturing the aerogel provided by this remuneration includes the upper shape of the city, the _ county solution is more complete and the high net thief _ product, even in the extraction In the program, the high-level interface of Zhang 13 1329032 is used as the solvent for the stroke. It will not cause the pore structure of the aerogel to disintegrate, but still maintain the high degree and the plant structure (咐财k). The above-mentioned ion (four) is a normal temperature ionic solution (R〇〇mt-cli_), which is made up of - organic test and - Louis ==. The new wheel she and her _ also moved into normal temperature ion refining, but this face The hydrogenation acid is produced on the water side and the water side, so that it is used in the present invention. Therefore, the use of the present invention is easy to use, thereby preparing an ion bond which is stable in the tree. In the present embodiment, a preferred example The general formula of the cationic group in the above organic test is as follows:

Among them, the choice of WU and R4 is shown in the following table:

R1 ~R7- r ~~~ CH3 Η ---- ch3 H C2H5 ch3 H C2H5 Η c2h5 H-CH3CH2CH2CH2 Η ch3 H (CH3)2CHCH2 Η ch3 H CH3CH2CH2CH2 Η C2H5 H — CH3 Η CH30CH2CH2 — H CH3 Η cf3ch2 H CH3 Ch3 c2h5 H CH3 ~αζ~~^ CH3CH2CH2 H ch3 ch3ch2ch2 H C6H6CH2 ch3 CH3CH2CH2CH2 H ." 12 c6h6ch2 ch3 (CH3CH2)(CH3)CH H c6h6ch2 ch3 CH3CH2CH2CH2CH2 H ch3 Γ H c2h5 ch3 C2H5 H C2H5 ch3 1329032 The more common organic cations include one of the following groups: ln-butyl-3 -methylimidazolium ( bmI ) , 1 -octanyl-3-methylimidazolium ( OMI ) , 1 -dodecanyl-3 -methylimidazolium ( DMI ) and l- Hexadecanyl-3-metiiylimidazolium (HDMI). Further, the anionic group in the above Lewis acid contains one of the following groups: bf4_, pf6_,

AsF6, SbF6, F(HF)n, CF3S03, CF3CF2CF2CF2S03 (TFSI), (cf3so2)2n, (cf3so2)3c, CF3CO〇 and CF3CF2CF2COO. When the type of the organic base used above is fixed, the hydrophilicity of the ionic melt can be controlled by adjusting the anionic group in the Lewis acid, for example, hydrophilic ion melt BMI-BF4 and hydrophobic ion melt BMI-(TFSI)2 . Taking Shixiyuan oxygen-silica/fresh recording monomer as an example, it hydrolyzes to form a hydrophilic (_Si_〇H) ' hemp, and the hydrophilic lining material and the stone alcohol attract each other, and can stabilize the cerium oxide. The formation of the structure gives a relatively stable dihydrated Wei gel. Since the hydrophobic ions repel the water, (4) also repels the oxyhydroxide in the sol and the oxyhydroxide oxime on the surface of the oxidized stone, so that it is less likely to attract each other and make the oxidation of the oxidized pin stable. Aerogels have larger group particle radii and smaller pore sizes. (S) 13 1329032 For the present application, the above ionic solution has a weight ranging from about at least one oxyoxy monomer and/or oxy-based monomer weight to 9 (10), preferably in the range of about 20% to 50%. In the present implementation, when the center of the silk-based mono/silk oxy-monomer is _ ό ό _, as the molecular weight of the non-alkali oxy group of the oxime monomer and the domain aryl oxy monomer increases, The gas condensing _ specific surface area also increases. Take the case of the oxycarbyl group and the BTSB lighter. The tetraethyl orthosilicate (TMOS). tetraethyl orthosilicate (TEOS) ^ bi_eAoxysilyl) e_e (the hall e (BTSB) as the precursor' can be found with the BTSE and BTSB light-driven The aerogel produced by __© is based on the aerogels of TMGS and TEOS _ surface 敎, push _ because it may be that the vinyl and btsb benzene ring groups of BTSE occupy the cyberspace in the aerogel, resulting in The number of holes in the aerogel increases, and the specific surface area also increases. The gas condensate green provided in this embodiment can be turned over to the preparation of the aerobic series of aerogels. The semiconductor materials have been researched for a while - especially In photocatalytic reflection, converting solar energy into chemical energy is more worthy of discussion. For example, semiconductor materials such as Ti〇2, ZnS and Sn〇2 are often used as photocatalysts, and titanium dioxide is most commonly used in photocatalytic reactions. Above - In general, the activity of the catalyst increases with the increase of its specific surface area. The lack of the traditional _• Ningxian Laibei titanium dioxide yield than the surface and pore volume 14

Oh,. However, the technique of shooting supercritical carbon dioxide, the sulphur dioxide, has a higher _ surface, and it is a fine _ trivial, fine _ high. In this example, the oxidized and oxidized ion of the material is used as a molten bar to form supercritical dioxygen (4), and a dioxane: gel having a large specific surface area and a large pore volume can be prepared. On the other hand, with the dihydrated Weigel, the network structure of the pure titanium dioxide gas condensate is relatively easy to shrink, resulting in a relatively low specific surface area and activity, so this embodiment uses an ionic solution as a template and a solvent. Taking a methoxyl monomer" or a scented ethoxy monomer [eg: her leg coffee _ 〇 收 伽

(TMOS)&gt;tetraethyl orthosilicate (TEOS)&gt;bis(triethoxysilyl)ethane (BTSE) ^bis(triethoxysilyl)benzene (BTSB)] / or aromatic oxy monomer [eg tetrabmyl &amp; followed by &amp; (tb〇t )] Copolymerization, made of -Oxygen-cut titanium dioxide (SiQ2_Tic>2) two-component aerogel to improve the pure titanium dioxide gas 郷 _ point, improve the application of titanium dioxide (four) on the catalyst 'in which 'Shi Xiyuan monomer The molar ratio of the monomer to the titanium source is 1:9 to 5: The method of manufacturing a surface-modified aerogel disclosed by the present invention first provides an aerogel precursor solution containing at least one alkoxylate. Alkaoxide and/or aryloxy monomer (IV) (IV) external catalytic hydrolysis and / or condensation reagent, -ionic liquid (with template and solvent function) and alkoxy groups with -specific groups And/or an aryloxy monomer, wherein the above 15 <S) 1329032 specific group comprises one of the following groups: a carboxylic acid group, a thiol group, an amine group, a diamine group, a hospital base (for example: Methyl group), an aromatic group (for example, phenyl group), an epoxy group, and a chaotic group. The above catalytic hydrolysis and/or condensation reaction reagent comprises one of the following groups or any combination thereof, an alcohol (carbon number less than or equal to 5), an acidic compound and a basic compound, thereby catalyzing the alkoxy monomer and / or an aromatic oxy monomer and an alkoxy monomer and / or an aryloxy monomer having a specific group are hydrolyzed and polymerized. In addition, another method for forming an aerogel precursor solution comprises: mixing a hospital oxymonomer and/or an aryloxy monomer, an ionic melt, and an alkoxy monomer and/or an aryloxy group having a specific group. The monomers form a first mixture. Next, an acidic compound is added to the first mixture and a second mixture is formed. Finally, a basic compound is added to the second mixture to increase the hydrolysis of the alkoxy monomer and/or the aryloxy monomer and the alkoxy monomer and/or the aryloxy monomer having a specific group. The degree of polymerization. Then, a curing process is performed on the aerogel precursor solution to facilitate the conversion of the alkoxy monomer and/or the aryloxy monomer and the alkoxy monomer and/or the oxy group monomer having a specific group. With the aggregation, take the job. The above curing procedure comprises a uniform mixing procedure and a standing procedure, first performing a _ uniform mixing procedure (for example, a strong meal or a strong shock) on the aerogel precursor solution to minimize the components in the precursor test, in particular Is a catalytic hydrolysis and domain condensation reaction reagent, so that the alkoxy monomer and / or aromatic oxygen monomer is fully hydrolyzed and polymerized 'the above uniform mixing procedure is continued until the aerogel_solution reaches 16 to - filament length, And the specific purity is greater than or #(9) eps, preferably greater than 2 is equal to 200 cps. After the completion of the homomixing process, the above-mentioned gas gelling solution is allowed to stand so as to continuously hydrolyze and polymerize the silk-based single-difficulty/oxyl monomer, thereby forming an aerogel. When the above-mentioned catalytic hydrolysis and/or condensation reaction reagent contains alcohol, the above-mentioned uniform age program includes a heating procedure, and the temperature is about 15 〇 C. The above-mentioned common components for aerogel formation include one of the following groups or any of them: 5 Si〇2, Ti〇2, V2〇5, and gossip 2〇3, and the aerogel has an average pore diameter ranging from about 1 nm. The specific surface area to 50 nm ' is greater than or equal to 丨〇〇m2/g, and the porosity ranges from 50% to 99%. Further, after completion of the above curing process, an extraction process may be performed by a solvent to replace the ionic melt contained in the pores of the aerogel. Next, a drying process is performed to remove the solvent contained in the pores of the aerogel. The above solvent is preferably selected from low boiling solvents having a boiling point of less than or equal to 2 Torr, including one of the following groups: nitriles, alcohols, ketones and water. Operation of the above extraction procedure The temperature ranges from about 5 CTC to 200. (: In the present embodiment, the selection of the central element in the above alkoxy monomer and/or aromatic oxy monomer, the selection of the acidic compound, the hydrolysis reaction and the dehydration polymerization reaction The temperature range, the kind of the ionic solution, and the addition amount of the ionic solution are selected in the same manner as in the first embodiment. Further, the above alkoxy monomer and/or scented oxymonomer having a specific group include the following group. One of them. 1329032 3-Aminopropyltriethoxysilane, N-(2-Aminoethyl)3-amino-propyltriethoxysilane (TMsen) '2-Cyanoethyltriethoxysilane (CNTS), Epoxypropoxypropyltriethoxysilane (EPTS) and Methyltriethoxysilane (METS)# Phenyltriethoxysilane (PHTS) ° - Why Need to modify the surface of aerogel? Take cerium oxide aerogel as an example of the surface of amorphous aerated cerium oxide. The _ 矽 hydroxyl group, which is called the surface of hydroxylation. The surface of this ruthenium-oxidized surface exposed to water will cause water to adsorb on the surface due to hydrogen bonding. Properties, specific surface area, hardness, etc., the right month b changes its surface chemical or physical properties through surface modification to produce unique applicability. For example: First, analysis: (4) column packing; (8) metal ion adsorption and separation. Catalysis: (4) direct catalysis of surface functional groups; (b) surface metal complex catalysis; (c) composite materials. 3. biological phase • application (a) biosensor (7) biochip. The gas coagulating method provided by the embodiment can be used for the preparation of the titanium dioxide series aerogel of Table 7. The other method, the application of the present invention, &gt; β surface modification aerogel manufacturing method The obtained aerogel can be made into two technical textiles, bio-composites, etc. by using knives/composite polymer electrolytes (CPEs), and knives/t:t*. Specific base The weight of the 7-oxyl monomer and the 2-aryloxyl monomer is about 20% to 50% by weight of the at least-o-oxyl monomer and/or 18 1329032 ^' aroma base. In the case of the second yard type, when the specific group is an amine group or a diamine group, the specific surface area and pore size of the modified aerogel decrease as the content of the amine and the diamine decane increases. On the other hand, the modified aerogel was subjected to adsorption test on metal ions, and it was found that the modified gas gel had adsorption capacity for both Cu(n) and Zn(n). As described in the first embodiment, when the central element species of the alkoxy monomer and/or the aryloxy monomer are the same, the non-alkoxy region of the alkoxy monomer and/or the aryloxy monomer is used. As the molecular weight increases, the specific surface area of the aerogel also increases. Therefore, the alkoxy monomer and/or the aryloxy monomer selected in the present embodiment has a plurality of central 7L elements to increase the specific surface area of the surface-modified aerogel, thereby enhancing the effects of adsorption, catalysis and the like. When the specific group on the metal oxide of the tomb is a stimuli group (for example, a methyl group) or an aromatic group (for example, a phenyl group), the modified aerogel is changed as the content of the saponin or the aryl group is changed. The specific surface area changes accordingly. On the other hand, the modified C gel was bubbled into an aqueous solution and found to be incapable of being submerged by water, demonstrating that the surface of the aerogel has changed from hydrophilic to hydrophobic. When the specific group on the metal oxide oxide is a functional group T (e.g., epoxy group) which can react with the radical, the modified agglomerate pores and the specific surface change with the change of the specific group content. In the above-described embodiments of the present invention, the present invention is composed of an ion immersion liquid having different properties and a paste A which has a specific amount of a gas enthalpy and a specific surface 19 1329032. On the other hand, the method for producing a surface-modified aerogel provided by the present invention can be applied to adsorption, catalysis, organic/inorganic composite materials and bio-aerogel composite materials. Accordingly, the present invention can meet economic benefits and industrial applicability. A third embodiment of the present invention discloses a method of manufacturing a biocomposite. The biocomposite has many fields of application, such as immobilized enzymes, drug controlled release, bioseparation techniques, sensors, and the like.

The immobilized enzyme (immobilized enzyme) is defined as "the enzyme is restricted to the special region, but does not hinder its active site (active 8 丨 (6) allows the enzyme to repeat the operation, reduce the amount of enzyme used, and will eventually The products are separated from the enzymes. The methods of gamification are usually divided into adsorption method, ion binding method, covalent bonding method, (four) method, embedding method, etc., in which the enzyme is immobilized on the support. The covalent bonding method has the advantages of _ reliance and high stability, and is the most suitable method for the determination of yeast. The fixed lucifer has the ability to use +, the rapid separation of energy and products, and the stability of enzymes. Advantages. Drug controlled release technology began to flourish in the 1970s, mainly through the transmission system 'to the rational, chemical, or biological methods to control the concentration of music in the ash, and then receive the impurities The concentration can be controlled to different degrees to achieve different therapeutic effects of the pharmacological gamma. In the drug controlled release of the research red, part A I force or change the material properties, '_ polymer material permeability to control the crane release The rate is most often 20 1329032 See: In the past, h $ is based on the characteristics of polymer water gel _ 彡 特性 来 碰 释放 释放 释放 = = = = = = = = = ' ' 高分子 高分子 高分子 高分子 高分子 高分子 高分子 高分子 高分子 高分子 高分子 高分子 高分子 高分子 高分子 高分子In the identification of biomolecules, the so-called Molecular Templates are separated from each other. Because different ages often have no coma, at this time it has a special input. 'If we want to separate this molecule from many different molecules, then the thief needs the right lock. In other words, the special material for screening a particular molecule needs to get the first target molecule as a template. The target molecule forms a complex with the effective monomer, and the crosslinking agent acts to form a polymerization reaction. After removing the template (the target molecule), the shape of the molecule can be formed in the structure of the polymer. Selective _ special molecular function. Materials made by molecular template technology often have a very high recognition function, can bribe or separate non-micro biomolecules such as protein f, _ Viruses, • Microorganisms, etc., are widely used in disease or food testing. If molecular templates and electronic technologies are combined, it is possible to design a thin film sensor with different molecules and the measured signal output for further analysis. In addition, the molecular template technique can also be applied to a binding reaction that mimics an antigen-antibody or an enzyme substrate, provides a catalytic action to facilitate a chemical reaction, develops an analytical tool for various biological studies or medical tests, etc. The biocomposite disclosed in this embodiment Material manufacturing method, first provided

21 (SJ - a precursor solution comprising - a hydrophilic melt, a catalytic hydrolysis and / or condensation reaction reagent, at least one biomolecule and at least one alkoxide and / or an aromaticoxy monomer ( Aryl〇xide), wherein the catalytic hydrolysis and/or condensation reaction comprises a buffer of the following scales or any age: alcohols and biomolecules, wherein the buffer has a pH range of about 5 to 9. Secondly, a curing process is performed on the precursor solution to facilitate at least one activating epoxy group and/or (tetra)oxy group to undergo reverse tactile polymerization, thereby curing and coating at least one biomolecule. To form a biocomposite. Then, the biocomposite is subjected to an extraction process by --reading the biocomposite to replace the ion test contained in the biocomposite. Then, a drying procedure is performed on the biocomposite to remove the biocomposite. The solvent contained in the above. When the above-mentioned biocomposite is used for separation purposes, there are two common forms: the first-type neo-biocomposite--crushing procedure to form a plurality of biocomposites Then, a plurality of biocomposites are filled in a separation column to form a bioseparation device. The second is to directly form the biocomposite on the column of the column (for example, capillary), and the other is Bioseparation device. The biomolecules described above include the following: antigen, monoclonal antibody, multi-drug antibody (p〇lyd〇nai antib〇dies), nuclear nucleic acid [including Monomeric (m〇n〇merjc) and oligomeric (oligomeric), protein (pr〇teins), enzymes, lipids 22, polysaccharides, sugars (SUgars) , peptides, polypeptides, drugs, viruses, microorganisms and bioligands. The above hydrophilic ion refining system is a normal temperature ionic liquid, which is preferred. From the first embodiment (for example: BMIC-BF4) to facilitate uniform mixing with hydrophilic micromolecules. The above-mentioned oxy- and/or aromatic-oxyl monomers, the selection of their central elements, and hydrophilic ionic melting The weight range of the liquid and the first real The same applies. The above-mentioned biocomposite has an average pore size ranging from about nm to 5 〇 nm. The specific surface area is greater than or equal to 1 〇〇 m 2 /g, and the porosity ranges from 99% to about 20%. The range is greater than or equal to 1 〇cm3/g. In the present embodiment, the above curing procedure comprises a uniform mixing procedure and a static private sequence, first performing a uniform mixing procedure on the aerogel precursor solution (for example: strong Mix or strongly oscillate) to disperse the components of the precursor solution as much as possible, especially the template-ion refining solution, so that the 6-oxyl monomer and/or the aromatic oxy monomer are fully converted, and the temperature is continued. The aerogel precursor solution is subjected to a specific reduction, and the woven fabric is greater than or equal to 15 〇 cps' preferably greater than or equal to 2 〇〇 cps. After the uniform mixing process is completed, the above-mentioned grades are transferred to the county liquid, and the bribery bribes are bribed/

The monomer continues to turn into a financial combination, thereby reducing the amount. I &amp; 实 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Good separation effect. For the activity of biomolecules, the choice of parameters and the scope of operation of the program are very important, for example: the temperature of the curing program is _ 5 (rc; the extraction depends on one of the rhyme groups or any combination thereof: alcohols, water and organisms) The buffer required by the molecule is nuffer), wherein the pH range of the retardation is 5 to 9. The temperature of the extraction procedure is less than or equal to 50 C; the drying procedure is a cold-drying procedure, the pressure of which is less than or equal to 2 〇 Pa. The temperature selection of the cold-drying procedure is related to the extraction solvent, and when the extraction solvent is water, the dry-drying procedure The temperature is less than or equal to 〇. 〇; When the extraction solvent is an alcohol, the temperature of the drying procedure is less than or equal to _2 〇. (: For example, 'When using DsRed as a molecular template and siliea aerGgel as a substrate, the bio-clad material test results are as follows: I-scanning Electron microscopy (SEM) test: The second figure shows the microscopic shape of the aerogel surface by scanning electron microscope (SEM). The magnification of the second graph (a) is 3〇k, and the cerium oxide is observed. The surface of the aerogel exhibits a spherical shape, and by observing the magnification of 300k in the second figure (b), it can be found that the above-mentioned spherical shape is composed of more and smaller dioxide, each of the cerium oxide. The size is about 2〇~3〇nm, which proves that the substrate is Nai's aerogel pore material. 3. Nitrogen adsorption desorber test: The third figure shows the cerium oxide aerogel (siHcaaer〇gel) ), the dioxide dioxide 24 矽 aerogel coated red $ moved from (Aerogel + DSRed) nitrogen adsorption desorption curve 'the prepared silica dioxide aerogel has a specific surface area of about 768 m2 / g, the pore volume is 2.1 cm3/g, the average pore size is 14 〇nm; after coating red fluorescein, specific surface area It is about 699 m2/g, the volume of the hole is 2 〇cm3/g, and the average pore size is 13.8 nm. It can be seen that the volume of the hole before and after coating is slightly decreased. The fourth figure can be explained by the fact that red fluorescein is Coated in a substrate. 4. Confocal Laser Scanning Microscopy (CLSM) Test: The fourth picture shows a confocal microscope image of a cerium oxide aerogel coated with red fluorescent protein. In the case of confocal microscopy, images of different depth levels of the sample can be obtained. These image information can show the vertical dispersion and embedding of the coated red fluorescent protein. The point distribution in the fourth figure is Red fluorescein' shows that the dioxide dioxide prepared by the method has good coating and uniform dispersion of red fluorescein (DsRed), and has been successfully prepared into a biological composite. Materials, this is an important breakthrough in the application of cerium oxide aerogel. 5. Fluorescence Spectrophotometer test: The fifth and sixth figures are red fluorescent protein (DsRed) and biological aerogel At room temperature 558 nm is the spectrum of the photoexcitation of the excitation light source. In principle, the aerogel doped with red fluorescent protein (DsRed) is measured by excitation light 558 nm and absorption 1322932 light 583 nm; doped green fluorescent protein ( The aerogel of EGFp is measured by excitation light 488 nm and absorption light 507 nm, and the survival is detected by a percentage ratio. The fifth figure shows that red fluorescent protein has three specific absorption peaks, and its activity increases with time. There will be a slight decrease; the sixth picture shows the activity detection of cerium oxide aerogel-coated red fluorescent protein, and it can be found that the degree of activity decline on the first day after coating is large, about 11%, but then The anaerobic aerogel skeleton is gradually constructed, and the red fluorescent egg is coated and protected. Therefore, the activity is gradually stabilized, even if it is removed by the plate, the activity is still returned. More than 70%, this provides a good development platform for future applications. Obviously, many modifications and differences may be made to the invention in light of the above description. Therefore, it is necessary to make a solution within the scope of the appended claims. In addition to the above detailed description, the present invention can be widely practiced in other aspects. The above are only the preferred embodiments of the present invention, and are not intended to limit the invention, and the equivalent changes or modifications made by the present invention in the spirit of the present invention should be included in the following application. Within the patent.

26 [mesh diagram] A mfr diagram according to a first embodiment of the present invention, a solid-state nuclear magnetic resonance spectrum analysis (solidstate 29si NMR) analysis results of a dioxide dioxide gas gel; the second diagram is according to the present invention In the third embodiment, the electron microscopy (SEM) photograph of the biocladding material (a) magnification is 3Q magnification 300k; the third graph shows the magnet dioxide aerogel (silicaaer〇gel), package Reddish

"The nitrogen adsorption curve of one of the protein oxidized stone aerated gel (Aer〇gel + DsRed); the confocal U-mirror image of the heart, 曰81Μ red-coated® light egg from the oxidized 7-aerogel; The sixth picture shows the spectrum of photoexcitation of the red fluorescent protein (DsRed) and the red fluorescein-coated oxidized stone cerium aerogel at 558 nm as the excitation light source at room temperature. S &gt; 27

Claims (1)

1329032 X. Patent application scope: * ~ 1.- The manufacturing method of the seed gas, the manufacturing method of Wei_ contains. Providing an aerogel precursor solution, wherein the aerogel precursor solution comprises a 1-ion solution, a catalytic hydrolysis And/or a condensation reaction reagent and at least one calcined lysate (-峨/丝魏体体_ 涵), a hybrid, a hybridization reaction and/or a fine reaction reagent comprising one of the following groups or any combination thereof: an alcohol, An acidic compound and a basic compound; ♦ a tenter gel precursor solution-uniform mixing procedure to facilitate hydrolysis and polymerization of at least one of the alkoxy monomers and/or aromatic oxygen until the aerogel precursor solution reaches - Specific reduction, the domain specific reduction is greater than the scale of 15 〇; the gas precursor solution is allowed to stand so as to continuously hydrolyze and polymerize at least the methoxyl monomer and/or the aryloxy monomer, thereby forming the gas condensation An ionic liquid contained in the pores of the gas condensate by means of a gas-dissolving surface--a procedural preparation. 2. The method for producing an aerogel according to the first aspect of the patent application, The above alkoxy monomer / or the central element of the aromatic oxy monomer comprises one of the following groups: Li, Na, K, Rb, Cs, Be, Mg, Ca, Sr, Ba, Ti, Te, Cr, Cu, Er, Fe, Ta, V, Zn, Zr, A, Si, Ge, Sn, and Pb. The method for producing an aerogel according to claim 1, wherein the above alkoxy monomer and/or aromatic oxy group The monomer comprises one of the following groups: Metramethyl orthosilicate (TMOS), tetraethyl orthosilicate (TEOS), bis (triethoxysilyl)ethane (BTSE) 'bis(triethoxysilyl)benzene 28 1329032 (BTSB), tetrabutyl titanate (TBOT) and vanadium oxytripropoxide. 4. For the manufacturing method of the gas _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ The aerogel manufacturing method according to the above item, wherein the ionic solution of the towel is formed by mixing an organic test with a Lewis acid, and the Lewis acid is not a halogenated acid. The method for producing an aerogel according to the above, It may be an alkyl group or an aromatic group, the following general formula: - which ^ R3 R4 shown: Ri-4n 29 c6h6ch2 ch3 CH3CH2CH2CH2 H c6h6ch2 ch3 (CH3CH2)(CH3)CH H c6h6ch2 ch3 ch3ch2ch2ch2ch2 H ch3 H c2h5 ch3 C2H5 H C2H5 ch3 7. The method for producing an aerogel according to claim 5, wherein the organic base cation comprises one of the following groups: (BMI) 1 -octanyl-3-methylimidazolium (〇MI ), l-dodecanyl- 3-methylimidazolium ( DMI ) and l-hexadecanyl-3-methylimidazolium (HDMI) ° 8. The method for producing an aerogel according to claim 5, wherein the above-mentioned Louis The anionic group in the acid includes one of the following groups: BF4_, ρ^·, AsF6- &gt; SbF6-, F(HF)n-^CF3SO3-, CF3CF2CF2CF2S〇36, (cf3so2)2n·, (CF3S 〇2) 3c, Cf3C00· and CF3CF2CF2 (: 〇a. 9. The method for producing qi according to the above-mentioned patent item 1, wherein the ionic melt has a weight ranging from about 10% to 90%. The method for producing an aerogel according to the above aspect of the invention, wherein the weight of the above-mentioned sub-solution is in the range of about 20% to 50%. The above-mentioned fixed viscosity is greater than or equal to 200 cps. "Special 12. If applying for planting (4) 丨 所 魏 魏 _ 制造 制造 制造 制造 制造 制造 制造 催化 催化 催化 催化 催化 催化 催化 催化 催化 催化 催化 催化 催化 催化 催化 催化 催化 催化 催化 催化 催化 催化 催化 催化 催化 催化 催化The method for producing an aerogel according to the invention of claim 12, wherein the heating process temperature range is 50 to 1503⁄4. The method for producing an aerogel according to the above application, wherein the solvent The boiling point is less than or equal to 200. K. The method for producing an aerogel according to the above-mentioned item 1, wherein the above-mentioned dissolution class comprises one of the following groups: a guess, an alcohol, an ugly water, and the like. 16. The method of producing an aerogel according to the above application, wherein said extraction step has a temperature in the range of about 50.匸 to 200. ^. 17=申, The method for manufacturing aerogel according to item i of the patent scope, in the above-mentioned extraction, after the completion of the process, the process of removing the gas contained in the gas-filled aperture towel is removed. The method according to the above, wherein the precursor solution further comprises at least a specific monomer and/or a fragrant-based monomer, the specific group comprising the following groups; The ones are: sharp group, thiol group, amine group, diamine group, alkyl group, aryl group, epoxy group and cyano group, thereby forming a surface-modified aerogel. The invention relates to a method for producing an aerogel according to Item 1, wherein the aerogel component comprises - or any combination of the following groups: SiO 2 , TiO 2 , V 2 〇 5 and Al 2 〇 3 . 31 丄wy〇32 20. As described in the scope of application of the scope of the patent, the gas gel is a two-dimensional gel of dioxobic dioxide (Si02_Ti02) and dioxide. The molar ratio of cerium to titanium dioxide is 丨: 9 to 5: 5. 21. The method of producing an aerogel according to the patent application, wherein the aerogel has a specific surface area greater than or equal to 100 m2/g. 22. The method of producing an aerogel according to the invention of claim 1, wherein the aerogel has an average pore passage range of from about 1 nm to about 50 nm. • A method of producing an aerogel according to claim 1, wherein the aerogel has a porosity ranging from 50% to 99%. 24. If you apply for a patent scope! The method for producing an aerogel, wherein the aerogel has a pore volume range greater than or equal to L 〇 cmVg. 25. A method of producing a biocomposite comprising: providing a precursor solution, wherein the precursor solution comprises a hydrophilic ion melt solution, a catalytic hydrolysis and/or condensation reaction reagent, at least A biomolecule and at least one alkoxide and/or an aryloxy monomer (IV), wherein the catalytic hydrolysis and/or condensation reagent comprises one of the following groups or any combination thereof: a buffer required for the alcohol and the biomolecule; performing a curing procedure on the precursor solution to facilitate the reaction of at least one of the alkoxy monomer and the /Wowei single ship And coating at least one biomolecule to form the biocomposite; and extracting the ionic liquid contained in the biocomposite by performing an extraction process on the biocomposite by a/valley. The method for producing a biocomposite according to claim 25, wherein the biomolecule comprises one of the following groups: an antigen, a monoclonal antibody, and a polyclonal antibody. Nucleic acids [including monomeric and oligomeric], proteins, enZymes, lipids, polysaccharides, sugars (SUgars) ), peptides, polypeptides, drugs, viruses, microorganisms, and bioligands. 27. The method of producing a biocomposite according to claim 25, wherein the central element of the above-mentioned methoxyl monomer and/or aryloxy monomer comprises the following groups: Li, Na, K, Rb, Cs, Be, Mg, Ca, Sr, Ba, Ti, Te, Cr, CU, Er, Fe, Ta, V, Zn, Zr, AhSi, Ge, Sr^Pb. 28. The method of producing a biocomposite according to claim 25, wherein the alkoxy monomer and/or the aryloxy monomer comprises one of the following groups: tetramethyl orthosilicate (TMOS) &gt; Tetraethyl orthosilicate (TEOS), bis(triethoxysilyI)ethane (BTSE), biS(trieth〇xysilyl)benzene (BTSB), vanadium oxytripropoxide ° 29. The method for producing a biocomposite according to claim 25, wherein the above The hydrophilic material is a fine ionic liquid. The method for producing a biocomposite according to the above aspect of the invention, wherein the hydrophilic ionic solution is mixed with an organic acid and a Lewis acid, and the Lewis acid is not a halogenated acid. The method of manufacturing the biocomposite material of the 25th item of the monthly patent range, wherein the weight range of the hydrophilic ion solution mentioned above is about secret. . Such as Shen. The method of manufacturing a biocomposite according to the above-mentioned patent, wherein the above-mentioned aqueous ionic melt has a weight of from 2% to 2%. The method for producing a biocomposite according to claim 25, wherein the buffer has a pH in the range of about 5 to 9. The method for producing a biocomposite according to claim 25, wherein the curing procedure comprises: performing a uniform mixing procedure on the aerogel precursor solution to facilitate at least one oxime and an oxyl monomer. Hydrolysis and polymerization with the /silk oxy- ing monomer until the aerogel precursor solution reaches - the specificity of the city 's mosquito woven is greater than or equal to 150 cps; The gas precursor solution is allowed to stand, and the oxirane-type oxy-compound and/or the aryloxy monomer are continuously hydrolyzed and polymerized, thereby forming an aerogel. % The method of manufacturing a biocomposite according to the above application, wherein the specific viscosity is greater than or equal to 2 〇〇 cps. 36. The method for manufacturing a biocomposite according to the application of the above-mentioned biocomposite is described in which the curing procedure temperature is less than or equal to 5 (rc. 八丁工37. If the patent application is fine&quot; The solvent type includes one of the following groups or any combination thereof: an alcohol, a buffer required for the biomolecule (buffer s〇iutiQnq), and a method for producing the biocomposite according to claim 38. The pH of the buffer described in the above 34 1329032 ranges from about 5 to 9. 3 The method for producing a biocomposite according to claim 25, wherein the temperature of the stroke program is less than or equal to 5 〇. /, Ding Mu muscle, as claimed in the patent application scope of the biological composite material manufacturing method, after the completion of the above procedure, the bio-composite material is «the silk compound contained in the bio-composite material. The method for producing a biocomposite according to Item 40, wherein the temperature of the drying procedure is less than or equal to 0. /, 42. The preparation of the biocomposite according to the fourth aspect of the patent application. The method of manufacturing a biocomposite according to claim 25, wherein the biocomposite has a specific surface area greater than or equal to 1 (10) to 2/. The method for producing a biocomposite according to claim 25, wherein the biocomposite has an average pore size ranging from about 11 to about 5 〇 1 ^. The method for producing a material, wherein the above-mentioned biocomposite has a porosity ranging from 50% to 99%. 46. If the application method is applied to the biological compound (4), the pore volume of the aerogel (p〇re) V〇lume) range is greater than or equal to ^ 〇 coffee 3 / bogey. " 35