TWI327645B - Markers for detection of gastric cancer - Google Patents

Description

1327645 IX. DESCRIPTION OF THE INVENTION: TECHNICAL FIELD OF THE INVENTION The present invention relates to cancer (4). In particular, the invention relates to the use of detecting the genetic and/or egg-to-f markers of cancer, and more particularly, for detecting the transmission of gastric cancer and/or the use of protein markers. [Prior Art] When early detection and treatment of cancer 'greatly improved the survival of cancer patients in the case of monthly cancer, diagnosed patients with early disease, 9〇% have a 5-year survival rate' and about 1〇 % of patients diagnosed with advanced disease compared. However, the vast majority of patients with gastric cancer currently have advanced disease. As a result, the development of early diagnosis of lunar cancer can lead to improved patient prognosis. Identifying markers associated with specific cancers in biological samples, including body fluids such as blood, urine, peritoneal rinses, and fecal extracts, provides a valuable means of early cancer, leading to early treatment and improved prognosis. Specific cancer markers can also provide tools to monitor disease and track the efficacy of surgery, radiation therapy, and chemotherapy. However, for many major cancers, the available markers suffer from insufficient sensitivity and specificity. For example, the most commonly used gastric cancer markers cal9_9, ca72_4 and chorionic embryonic antigen (CEA) detect only about 15-50% of any stage of gastric tumors' and decline to about 2%% for early disease. Therefore, there is a very high frequency of false-negative tests that can lead patients and caregivers to believe that no disease is present, but in fact patients may have serious cancers that require immediate attention. In addition, these markers may be rarely over-expressed in up to 1/3 of patients with benign gastric disease 95238.doc 1327645, allowing only selective use of GTM measurements, selective and accurate detection In other embodiments, 'two, three or more Gtm can be detected in a sample and provide greater diagnostic certainty." The selected genes encoding the protein can be screened by or excised from the cells. Proteins f 'separately or in combination with each other' have a serum or body fluid marker for diagnosing gastric cancer' or as a marker for monitoring established diseases. Protein markers can be detected using methods known in the art. Including the use of monoclonal antibodies, multiple antisera and their class (IV), using radioimmunoassay (RIA), protein wafers or suspension arrays. [Embodiment] Definitions Before describing specific embodiments of the invention, some are provided herein. The definition of the noun used in it will be useful. The term "GTM" or "stomach tumor marker" or "GTM family member" means the meaning of gastric cancer. Genes, gene fragments, RNA, RNA fragments, proteins or protein fragments 或 or other molecules known to be associated with gastric cancer in the prior art associated with it = CEA. Examples of GTMS are included below. •, marker &quot ;- The word means that the κ of the knife marker that is qualitatively or quantitatively related to the existence of the biological phenomenon is GTMs, however the "marker" also includes metabolites, by-products, either directly or under conditions concealed under the mechanism Indirectly related. The term qPCR is intended to specify the amount of a polymerase chain reaction. 95238.doc 1327645 "Performance" includes the production of mRNA from a gene or a portion of a gene, as well as the production of a protein encoded by an RNA or gene or a portion of a gene, including appearance and expression. Relevant test materials. For example, binding to a ligand, such as an antibody, binds to a gene or other oligonucleotide, protein or protein fragment, and causes the binding partner to develop color, and is also included in the term "expression." For example, increasing the density of spots on western blots is also included in the range of the term 'performance' hidden under biomolecules. "CPN2" - Word means human carboxypeptidase N, polypeptide 2,83 kDa chain; and carboxypeptidase N. The term "HAPLN4" means human hyaluronan glycoprotein linked protein 4. The term "MMP 12" means human matrix metalloproteinase 12. The term "INHBA" means human statin, beta Α (also includes activin A, activin ΑΒ or alpha polypeptide). "IGFBP7" means human insulin-like growth factor 7. The term "GGH" means human gamma-amidoxime hydrolase (also known as conjugase, folylpolygammaglutamyl hydrolase). The term 'LEPRE1' means human proteoglycan rich in leucine proline (also known as leprecan 1). "CST4," means human cysteine protease inhibitor S . nSFRP4" — The word means human secreted frizzled related protein 4 〇"ASPN" means human asperin (also known as LRR class 1). 95238.doc -9- 1327645 ' 'CGREF1" or "CGRii" - the word means a human cell growth regulator having the EF hand functional site i. "KLKn - the word means human kallikrein 1 〇, variant 1 or human agonistic release unless otherwise specified Enzyme 10, variant 2 or both. "TIMP1" - The term means a human tissue inhibitor of metalloproteinase 1 (also known as a red jk ball-potentiating activity or a protease inhibitor). "SPARC" - Word means human secreted protein, acidic, rich in cysteine (also known as 〇ste〇nectin). The term "ru i'TGFBI" means human transforming growth factor, induced by 68 kDa. The term "EFEMP2" means human-containing EGF-like fibuUn extracellular matrix protein 2. "LUM" - The word means human lumican. "SNN" - The word means human stannin. SPP1" - Word means human secretory phosphoprotein 〖 (also known as osteoporin (〇Ste〇P〇ntin) or bone sialic acid protein 1 or early τ_lymphocyte activation 1) 0 CSPG2 - clever means human The chondroitin sulfate proteoglycan % is also known as versican. "ASAH1"-word means human N_indolyl alcohol amine hydrolase, variant 1 or N-mercaptosphingosine guanamine hydrolase, variant 2 or halope sphingosine hydrochloride hydrolysis Both enzyme variants 1 and 2 (also known as acidic neural guanylase, variant 2). 95238.doc -10- 1327645 "PRSS11" - The word means human protease, serine 11 (also known as IGF binding serine protease). "SFRP2" - word means human secretory curl-related protein 2. "PLA2G12B" means the human phospholipase A2, XIIB population. The term "SP0N2" means human spondin 2, an extracellular matrix protein. The term "OLFM1" means human olfactomedin 1 . "TSRC1" - The word means a repeat of the human thrombospondin containing 1. The "THBS2" word means human thrombospondin 2. The word "Adelicon" means DKFZp564I1922. "CST2" - The word means the human cysteine protease inhibitor SA. nCSTl" - the word means the human cysteine protease inhibitor SN. "L0XL2" means human adenosine oxidase-like enzyme 2 . The term "TG" means human thyroglobulin. The term "TGFB1" means human transforming growth factor, beta ΐ. "SERPINH1 ” means human serine or cysteine protease inhibitor, level Η (Also known as heat shock protein 47, member 1, or collagen binding protein 1). "SERPINB5" - The word means human serine or cysteine protease inhibitor, level Β (also known as ovalbumin, member 5). The term "CEACAM5" or nCEA" refers to human carcinoembryonic antigen-related cell adhesion molecule 5 .

"MMP2" - the word means human matrix metalloproteinase 2 (also known as gelatinase A 95238.doc 11 丄 to 7645 or 72 let 〇3 gelatinase or 72 kDa type IV collagenase). PCSK5 - word means human protein The original transforming enzyme subtilisin, kexin (type 5). It should be understood that the above terms may refer to proteins, DNA sequences and/or RNA sequences. It should also be understood that the above nouns may also mean Non-human protein, DNA, and/or 111 VIII of the same sequence as described. Description of specific embodiments of the present invention Long-term speculation and af evaluation of cancers including cancer of gastric cancer, which have a ratio in detecting gastric cancer First of all, the identification of the art is more reliable. Regarding the word "reliability", we include the lack of false positives and / or false negatives. Therefore, the higher the reliability of the marker, the diagnosis with the use of the marker The less false positives and/or false negatives are associated. Thus, in some embodiments, the identification of gastric cancer is provided to allow for the conviction of about 5% higher than the reliability of prior art markers. In other A marker having a higher reliability than about 70°/. is provided in the embodiment; in another embodiment, it is higher than about 73%; in another embodiment, it is higher than about 80%. In another specific embodiment, it is more than about 9〇%; in another specific embodiment, it is higher than about 925%; in another specific embodiment, it is higher than about 98°/. And in some specific embodiments, a marker of about 100°/. Reliability. Therefore, we have surprisingly found that the appearance of many genes and proteins is associated with gastric tumors. Therefore, gene products can be used (eg, nuclear collection) Glycosylates, such as mRNA), and the detection of proteins and peptides translated from such oligonucleotides to diagnose tumors such as gastric tumors. Patients from patients with gastric tumors and phase 95238.doc •12· 1327645 Array analysis of individual non-malignant tissue samples has led us to the surprising discovery that 'in many stomach tumors' specific patterns of overexpression of certain genes are associated with disease. And raised antibodies, check Measuring cancer markers. Therefore, by analyzing the presence and amount of multiple cancer markers, the sensitivity of the diagnosis can be increased while reducing the frequency of false positive and/or false negative results. The general method of cancer detection The following method is used A member of the GTM family, a non-limiting method for detecting cancer, including gastric cancer. • A microarray using oligonucleotide probes that are selective for the GTM gene product. • Use primer-specific primers and probes, Immediate quantitative PCR (qPCR) was performed on tumor samples and normal samples. • Enzyme-linked immunoassay (ELISA) Immunohistochemistry was performed on gastric tumors and lymph node metastasis using anti-marker antibodies. • Immunohistochemistry is performed on other tumors including, but not limited to, colorectal, pancreas, ovary, melanoma, liver, esophagus, bladder, endometrium, and brain using anti-marker antibodies. • Immunological detection of marker family members was performed before and after surgery to remove tumors from serum + ' taken from gastric cancer patients. • Immunodetection of marker family members in healthy individuals and in sera from individuals with non-malignant diseases such as gastritis, ulcers, stomach, and dysplasia. • Perform immunoassays for members of the marker family in patients with other cancers including, but not limited to, colorectal, visceral, nest, piano, pigmented tumor, liver, esophagus, bladder, endometrium and brain. In body fluids, including serum, lymph, peritoneal fluid, cerebrospinal fluid, synovial fluid, and the like, markers are detected. • In the gastric juice, peritoneal lavage fluid 'urine and feces from gastric cancer patients', the immune test of the members of the genus is performed. Array methods and/or qPCR are used. • Use a computer to analyze arrays or qPCR data. Raw data were collected and analyzed for fold change by comparing the degree of monthly tumor gene expression with the extent to which the same gene was expressed in non-tumor tissues. A threshold is established to conclude that the performance is increased (e.g., 1.5x increase, 2-fold increase, and in other embodiments, euploid increase, 4-fold increase, or 5-fold increase). It will be appreciated that other thresholds for determining that an increased performance has occurred may be selected without departing from the scope of the invention. Further analysis of tumor gene expression involves matching the genes showing increased performance to known performance profiles of gastric tumors to provide a diagnosis of the tumor. In certain aspects, the invention provides methods of detecting cancer, comprising: (a) providing a biological sample; and (b) detecting excessive performance of a gtm family member in the sample. In other aspects, the invention encompasses the step of detecting the overproduction of Gtm mRNA. In other aspects, the invention encompasses the step of detecting the overproduction of a Gtm protein. 95238.doc -14· 1327645 In a still further aspect, the invention encompasses the step of detecting excessive expression of a GTM peptide. In a still further aspect, the invention includes a device for detecting a GTM, comprising: a substrate having a GTM capture reagent thereon; and a detector coupled to the substrate, the detector capable of detecting a GTM associated with the capture reagent Wherein the capture reagent comprises an oligonucleotide or an antibody. Additional perspectives include kits for detecting cancer, including: Substance; ^ GTM capture reagents, including one or more GTM-specific oligonucleotides and GTM-specific antibodies; and instructions for use. A still further aspect of the invention comprises a method of detecting GTM using qPCR, comprising: a forward primer specific to the GTM; a reverse primer specific to the GTM; a PCR reagent; a reaction flask; and instructions for use. Additional aspects of the invention include a kit for detecting the presence of a GTM protein or peptide, comprising: a substrate having a capture reagent for the GTM protein or peptide; an antibody specific for the GTM protein or peptide; capable of indicating that it has been associated with the GTM protein or Reagents for peptide-bound antibodies; 95238.doc -15- 1327645 and instructions for use. An additional aspect of the invention comprises a method of making a monoclonal antibody comprising the steps of: In a further aspect, the invention comprises a method of detecting gastric cancer comprising the steps of: providing a sample from a patient suspected of having gastric cancer; using ELIS Method A measures the presence of GTM protein. As described herein, tumor detection can be accomplished by measuring - or the performance of multiple tumor-specific markers. We unexpectedly found that the association between increasing GTMs performance and diagnosing the presence of gastric cancer is extremely high: the least significant association was found with Am.6xl (r, p values. At a P value less than 20' many The association is significant. With such high significance, it may not be necessary to detect increased performance in more than one GTM. However, the lengthy GTMs in the present invention allow for the detection of gastric cancer with increased reliability. The methods provided also include highly sensitive assays. Grabs are extremely sensitive and can be used to detect gene products in very small copies (eg, 100) in samples. With such sensitivity, it is possible to Very early detection of gastric cancer-related events. Methods The following general methods were used to assess the suitability of various methods for molecular confirmation of markers associated with gastric tumors. Tumor Collection 95238.doc -16· 1327645 From at Seoul National University Hospital , and the surgical specimens removed from Korea and Dunedin Hospital, New Zealand, collected gastric tumor samples and non-malignant stomach tissue. Symptoms, scientific findings, and histological histology Based on the examination, the diagnosis of gastric cancer is performed. RNA extraction In some embodiments, the expression of genes associated with gastric tumors is analyzed by determining changes in RNA obtained from samples taken from tumors. The frozen surgical specimen was embedded. A 60 micron slice was cut from the tissue block using a microtome, homogenized in a mixture of TriReagent:water (3··1), and then extracted by gas chromatography. Then use the RNeasyTM program (Qiagen). Total RNA was purified from the liquid phase. RNA was also extracted from 16 cancer cell lines and pooled as reference RNA. Microarray slide preparation Epoxy coated slides were obtained from MWG Biotech AG, Ebersberg, Germany, and According to the manufacturer's draft, approximately 30,000 50-body oligonucleotides were printed using the Gene Machines microarray robot. The relevant oligonucleotides were shown in Figure 2, along with the alignment number of the NCBI mRNA and protein reference sequences (MWG). Oligo#). The complete DNA sequence of the GTM of the present invention is shown below. RNA labeling and hybridization in a reaction containing 5-(3-aminoallyl)-2'-deoxyuridine-5'-triphosphate, Use S Uperscriptll reverse transcriptase (Invitrogen), transcribes cDNA from 10 micrograms of total RNA. The reaction is then deionized in a Microcon column, followed by Cy3 or Cy5 95238 in bicarbonate buffer solution at room temperature. Doc -17- 1327645 Cultivate together for 1 hour. Using a Qiaquick column (Qiagen), remove the unincorporated dye and concentrate the sample to 15 μl in SpeedVac.

The cDNAs indicated by Cy3 and Cy5 were mixed with Ambion ULTRAhyb buffer solution at 100. (: Denaturing for 2 minutes, and at 16 ° C, in the hybridization chamber with the microarray carrier for 16 hours. Then rinse the slide and scan 2 times on the Ax〇n 4000A scanner set to 2 output Original fluorescence data for gene expression β normalization procedure to compare the performance of cancer genes from tumors and non-cancerous tissues, and to correct the intermediate light intensity detected by GenepixTM software by subtracting the local background fluorescence intensity Excluding points with background correction strength below 〇. To make standardization more convenient, logarithmically convert the intensity ratio and the overall dot intensity. For (4) and spatial skew, use the local regression provided in the lqcfitTM package to correct The ratio of the intensity of the logarithmic transformation. The intensity and position of the ink point is turned off, and the intensity ratio of the logarithmic transformation is simultaneously converted. The residual of the score is provided by the corrected log-fold change. The ratio of the array to the intensity of the dot and the plot: then check the dot plot with the naked eye for the remaining finished products. This ', Applied Variance Analysis (AN〇VA) mode normalizes all Large skew. In the library. > Number of inserts statistical analysis of Postgres-data statistical analysis by measuring the fold change in S in P fish; 5,1 4 regular tissue, confirming that the tumor sample is on the gene expression The statistical change significantly. To complete the work 95238.doc 18 1327645, proportionally adjust l〇g2 (proportional) to make each array have the same overall standard deviation. The standardization process reduces the variability within the average tissue type. Further convert log2 (proportion) so that each oligonucleotide has an intermediate value of 〇 to make the visual inspection result easier. Then use the level-based verification based on the fold change to improve the robustness of the voice. The two steps consist of (1) calculating the level of the fold change (Rfc) within the array, and (Η) subtracting the median value (Rfc) of the normal tissue from the median value (Rfc) of the tumor tissue. The difference defines the score that appears in the fold change level in Figure 2. Two additional statistical tests can be performed on the normalized data: 1) Student's t-test for two samples, with or #B〇nferr〇n^ ' And 2) Wilcoxon check. Statistical analysis of marker combinations To determine the use of a combination of two or three markers to identify the value of tumor and non-malignant samples, so that 40 pairs of samples of tqPCR data (tumor and non-malignant samples were obtained from the same Patient) received the following analysis. Using the sample mean and standard deviation, a standard distribution of non-malignant and tumor samples was generated. It is then determined that the value taken from the tumor performance data will exceed a defined threshold in the non-malignant distribution (eg, greater than 50%, 7〇%, 73%, 8%, 9%, 95%, 98%, 99% or Probability of 100%) (ie, sensitivity to the combination of markers, determining the likelihood that at least one marker exceeds the threshold. 95238.doc 19 1327645 95238.doc Class 1 transforming growth factor, ρ·transduced 1 1 osteogenic protein i 1 human inhibin PA chain 1 secreted structurally related protein 4 1 | containing egf • Zhuangbulin extracellular matrix protein 2 Π | Adelie Kens human human serine or cysteamine chymotrypsin inhibitor, Η 基质 1 Matrix Golden Epilepsin 2 1 person "Proteoplasmic mutated gull stem filling protein / Kaixin type 5 1 transforming growth factor β ΐ 1 human silk amine or cysteamine tt protease inhibition shot * level B | Human cell growth regulator I thyroglobulin ^ 1 thrombospondin 2 1 | tyrosine protease IG (IGF binding) | | secreted protein, acidic · cysteine-rich 1 | Secretory curl-associated protein 2 1 |N·mercaptosphingosine-enamined hydrolase 1 | Metalloproteinase inhibitor 丨1 | Matrix metalloproteinase 12 1 | Amine tt oxidase - Enzyme 2 1 1 Lumiken 1 | «Proteoglycan 1 (3⁄4 Puken) 1 with leucamine ML guanamine Peptide release 醻丨0 1 | insulin-like growth factor binding protein 7 1 | γ · amidoxime hydrolase | | cysteine peptone inhibitor SN, SA & SI | thiopterin chondrofovidin 2 (Fosiken) 1 | Aspen (Irr Dijon) 1 Brother | TGFB1 1 1 sppi i 1 INHBA I SFRP4 1 | EFHMP2 1 SERPINHI Γ MMP2 1 PCSK5 1 TGFB1 1 SERPINB5 1 CGR11 1 〇1 THBS2 1 1 PRSS11 1 | SPARC 1 1 SFRP2 1 1 ASAH1 1 1 TIMP1 1 1 ΜΜΡ12 1 1 L0XL2 1 | LUM 1 1 LEPREl 1 1 KLKIO 1 1 IGFBP7 1 1 GGH 1 丨CST1,2,4 | 1 CSPG2 1 1 ASPN 1 Symbol | Hs0〇165908_ml 1 Hs00167093_ml 1 Hs00l70103_ml | | Hs002l3545_ml | | HsOOS77849_ml 1 Hs00241844_ml Mi s ITTGATGGCATCGCTCAGATC | AAAAATCTTTGCCGGAAATGC [GGTCCATGTCATCACCAATGTT I TCCACGCATTTTCCAGGATAA [CTGCCCACCCCTTCCA | IGACGGTTCCTCGCAGTTCAA | Itggaaggactacacggcctatag | ITCGGGAGGCCC GTTAGTAA | [TCTTCCCTGTACACTGGCAGTTC I | CGCTAGCAGCGACCACCT | | CGCAGAACGCCTGCAAA | & AGACCACCTTATACCAGCG I [gcctctctgctgatgacatacgt I 1AGGCC AGCTTCTGCTTGG A | IGATTCTTGTCCATAGTGATCTGC | [CTTGAGTACAACGCTGACCTCTTC | | ACAACATGATATGTGCTGGACTGG | | CAGGTC AGC AAGGGC ACC | 1GTGGC AATGCCGCTGAA | [agtcccagcccaacttgga I; GCCAGTGG AATG ATGTTCCC | AAATACAAAAGGACAC ATTCAAAGGA! ! | forward primer 1_ to 〇〇〇 \ to < s > 00 • ο billion \ U to a sequence number ITGTCACGTGGCGTCACAGT 1 AGTCCTGGCCGTTGAAATACC Itctgcaagttcatcccctcttt I AAGCCGAATTTGCTAGTTGCA LTTCTGTCCTTCCTAGTCCCTTTAGG I ITGTAAACCGCTCCACTTCACAT I ITAGGTTTGGTC ATAG ATAGGTCCTG AGT1 lAAGGAGATTCCAGCTGTCACTTTC I IGAAAAAGCGGGTGGTGCA | [TTTTGCAGGCTTCACATACCTTT I lACAGGACATCATACATGGTTTCAAA I IGGACCTGTGGAAGTATCCGC I iAGTGACAGCATCAAAACTCAAATTG I ICCCTGATCGCCGAGTTG | CCAATCAATGCCAGGAAGAGA _I iCCGTGACACAGTTCTGCTTACAG | IGAGAGGATGCCTTGGAGGGT | | TC AC AGCTCAAGTAC ACCTGGG 1 TGACAGCAACAACTCAGTAGGAAAA 1 iGGGAACTTCGTAGA TCTGGAAAGA I iTCTTGGCATTTTCTACAACAGGG I TGCTTCTGCAATTCTGATATGGA 1 reverse 5 Shu sub 1 K > 00 σ \ u »KJ w hj 00 μ Guo B Cheng umbrella Ittcaaggaccggttcatttggcg | ACAGAATGTAGGGATGGGTTAAGCCTGCA ICAGCCTCCAGCCAACAGACCTCAGG | TGACTCCAGGCCCGCAATGGA ICCAGGCCAGGAGCAGCTCGG | ITCTGGCAGATTCCGATGCCCCACAA | Iaggcccaagaccggctacatcagagtc I lAGTGTTAATTCCAATCACTTCACCGTCCAGG I | TGGACCAGCACCCCATTGACGG | ICTGCCAGCCACCGAGGAAGCTC | ITGTCTGAACCGCACCAGCCAAGAGAATA I ICAAGATGACCAAGATGTATAAAGGGTTCCAAGC I ! | TCAGTCCCTGTATGGAGACCCAAAAGAGAA | iCGTAATTCTTCTGGATGTCTCCTTCACATTCTG I Itaaggattcaaaccatttgccaaaaatgagtctaag I 1CCATCACAG ATCATTACATCCAGGTCCTCA | ICTTGCCAGAGTGACTCTGGAGGCCC | | AGCAAGGTCCTTCCATAGTG ACGCCC | TTCACTGGAGGTCAATTGCACAGCAGAAT | Iagccagaactgcagaagaaacagttgtgc I i aggaacagttgcttgcggccagc I TTGGAAATGAGTGCAAACCCTCTTGATAATAATG 1 probe S: S Ον § Ό oo V / i ro VI di-long sequence encoding -20-1327645

Quantitative Real-Time PCR In other embodiments, immediate or quantitative PCR (qPCR) can be used for absolute or relative quantification of the number of PCR template copies. TaqmanTM probes and primer sets were designed using primer Express V 2.0TM (Applied Biosystems). Where possible, all potential conjugative variants are included in the resulting expansion zone, and the extension zone preferentially provides the area covered by the MWG-Biotech-derived microarray nutrient acid. Alternatively, use these if you want to represent the stem gene by covering the current array (Applied Biosystems). In Table 1 and in Fig. 1, the names of the genes, symbols, and Applied Biosystems" required to perform the PCR, the forward primer, the reverse primer, and the probe sequence are shown. In the assays designed in the company, the SYBR green labeling draft and the cDNA 'titration primer concentration made from the reference RNA were used. The expansion was performed on the ABI PrismTM 7000 Sequence Detection System under standard cyclization conditions. When a single amplified product was observed in the dissociation curve, a 5' FAM-3'TAMRA tartaric acid needle (Proligo) using an optimum primer concentration and a final concentration of 250 nM produced a standard curve in the range of more than 625-fold. In the subsequent assays, using a standard curve providing a regression curve with a regression coefficient of more than 0.98, it can be understood that in other embodiments, the regression coefficients need not be as high. More specifically, any standard curve can be used as long as the regression coefficient is high enough to allow a statistically significant determination of the difference in performance. In alternative embodiments, such regression coefficients can be about 0.7 or greater, about 0.8 or greater, about 〇.9 or greater or about 〇 95 or greater. Assays were performed on two 96-well plates, with a single cDna representing each 95238.doc -21 - 1327645 RNA samples. Each plate contains duplicate reference cDNA standard curves, exceeding the 625-fold concentration range. The analysis included the calculation of Δ(:τ (target gene ct_average reference cDNA CT). ACT is directly proportional to the change in the negative l〇g2 fold. Then the 丨〇g2 fold change relative to the intermediate non-malignant l〇g2 fold change is calculated. (1〇§2 fold change-intermediate standard l〇g2 fold change). These fold changes are then grouped into frequency categories and graphed. Microarray analysis of cancer marker genes is identified by Cy5 from 58 gastric tumors and 58 non-malignant ("normal") gastric tissue samples of RNA, and hybridized in duplicate or triplicate to the reference RNA indicated by Cy3. After standardization, then 29,718 genes were estimated by three measurements. In each of the changes in performance: (i) multiple ^ ik : the median value of the gene in the tumor sample divided by the ratio of the median value in the non-malignant sample. A significant fold change was observed. (ii) multiples of the syllabus and (iii) statistical probabilities 免疫 immunological analysis of cancer marker genes to understand that once antibodies against GTM (single or multiple plants) are produced, several types of immunity can be used. Learning method, GTM Detection. Radioimmunoassay (RIA) is well known in the art and can be used to detect and quantify GTM proteins in fluid or tumor extracts. Alternatively, protein wafers and/or suspension arrays can be fabricated. Where the antibody against GTM is placed on a substrate, such as a glass or plastic surface. The sample can be applied to the wafer surface or to the array of suspensions, and the GTM present in the sample is bound to the antibody. After unbound antibody, GTM can be developed using a second antibody against GTM. Methods for making antibodies and proteins 95238.doc -22- 1327645 wafers, and suspension arrays, known in the art, It is not further illustrated herein. For the selection of serum markers for gastric malignancy, in certain embodiments, a cancer marker can be found in biological fluids, including serum, (1) a signal sequence specific to or excised from the outside of the membrane. Exist, (... intermediate degree of overexpression (fold change) in the tumor compared to the non-malignant control group, (iii) between the tumor and the non-malignant control group Intermediate markers of performance, and (iv) selection of serum markers from array data based on the degree of overlap between tumor and non-malignant control ranges. All 29 GTMs are known to be in their passwords. The 5' end of the sequence has a signal peptide sequence. The nickname sequence targets the GTM protein and is transported via the serosal membrane to the extracellular compartment (Gunner von Heijne, Journal of Molecular Biology 173: 243-251 (1984)). Furthermore, there are no GTMs. Has a penetrating membrane sequence motif that will result in the full length of the protein being retained within the plasma membrane. As a result, all of the GTM markers of the present invention may be secreted into the extracellular space' and thus may be in contact with the blood supply, taken up by the capillaries or transported into the lymphatic system' and then into the blood supply. As a result, these tumor-derived markers will all appear in the blood. The gene was subsequently excluded if '50% of the tumor samples showed a degree of expression within 95% of the non-malignant range. In tumor samples, changes in the extent of overexpression not only reflect tumor heterogeneity, but also the extent to which tumor samples are contaminated by "normal" tissues, including muscle, stromal cells, and non-malignant epithelial glands. The change ^ "normal" pollution ranged from 5 to 70% with a median value of 95238.doc -23- 1327645 of approximately 25%. Exclude other genes because of their high relative expression in hematopoietic cells or elevated expression in metaplastic stomach tissue. It can be appreciated that depending on the extent of contamination by normal cells or cells that normally exhibit the marker, different threshold ranges can be selected to provide adequate separation of the cancer source from the normal source. We found useful GTMs including the following markers of genes (DNA), complementary DNA (cDNA), RNA, proteins and protein fragments: carboxypeptidase N, peptide 2, 83 kDa chain (also known as carboxypeptidase N (CPN2) ), matrix metalloproteinase 12 (MMP12), statin ("INHBA"), insulin-like growth factor 7 ("IGFBP7"), γ-glutamine hydrolase ("GGH"), rich in leucine Amino acid proteoglycan ("LEPRE1"), cysteine protease inhibitor S ("CST4"), secretory curly-related protein 4 ("SFRP4"), Aspen cloud ("ASPN"), with Cell growth regulator ("CGREF1"), kallikrein 10 ("KLK10"), tissue inhibitor of metalloproteinase 1 ("TIMP 1"), secreted acid rich in EF hand function site 1 Cysteine protein ("SPARC"), transforming growth factor, β-induced ("TGFBI"), EGF-containing phenanthrene extracellular matrix protein 2 ("EFEMP2"), Lumiken Yu ("LUM"), Stern ("SNN"), secretory phosphoprotein 1 ("SPP1&quo t;), chondroitin sulfate proteoglycan 2 ("CSPG2"), N-mercaptosphingosine guanamine hydrolase ("ASAH1"), serine protease 11 ("PRSS11"), secretory curl- Related Protein 2 ("SFRP2"), Phosphorus A2, XIIB Group ("PLA2G12B'1), Statin 2, Extracellular Matrix Protein ("SP0N2"), Olmestomide l("〇 LFMl"), a thrombospondin repeat containing 1 ("TSRC1"), human thrombospondin 2 ("THBS2"), Adelicon, cysteine protease inhibitor SA ("CST2n), half Cysteine protease 95238.doc -24· 1327645 Inhibitor SN ("CST1"), adenine oxidase-like enzyme 2 ("LOXL2"), thyroglobulin ("TG"), transforming growth factor , pi("TGFBl"), serine or cysteine protease inhibitor, level H ("SERPINH1"), serine or cysteine protease inhibitor, level B ("SERPINB5") , matrix metalloproteinase 2 ("MMP2"), proprotein convertase subtilisin / Kaixin type 5 (&quo t; PCSK5"), and hyaluronan glycoprotein-linked protein 4 ("HAPLN4"). The DNA sequence of the GTM of the present invention is shown below, together with confirmation information. Matrix metalloproteinase 12 >gi丨4505206丨ref | ΝΜ_002426·1丨 Human matrix metalloproteinase 12 (macrophage elastase) (MMP12), mRNA丨qPCR advancement—priming matches with [758..780] | qPCR reverse _ The primer matches [888..864]|9卩0 feet probe matches [786..815]

TAGAAGTTTACAATGAAGTTTCTTCTAATACTGCTCCTGCAGGCCA

CTGCTTCTGGAGCTCTTCCCCTGAACAGCTCTACAAGCCTGGAAAAAAAT

AATGTGCTATTTGGTGAGAGATACTTAGAAAAATTTTATGGCCTTGAGATA

AACAAACTTCCAGTGACAAAAATGAAATATAGTGGAAACTTAATGAAGG

AAAAAATCCAAGAAATGCAGCACTTCTTGGGTCTGAAAGTGACCGGGCAA

CTGGACACATCTACCCTGGAGATGATGCACGCACCTCGATGTGGAGTCCC

CGATCTCCATCATTTCAGGGAAATGCCAGGGGGGCCCGTATGGAGGAAAC

ATTATATCACCTACAGAATCAATAATTACACACCTGACATGAACCGTGAG

GATGTTGACTACGCAATCCGGAAAGCTTTCCAAGTATGGAGTAATGTTAC

CCCCTTGAAATTCAGCAAGATTAACACAGGCATGGCTGACATTTTGGTGG

TTTTTGCCCGTGGAGCTCATGGAGACTTCCATGCTTTTGATGGCAAAGGTG

GAATCCTAGCCCATGCTTTTGGACCTGGATCTGGCATTGGAGGGGATGCA

CATTTCGATGAGGACGAATTCTGGACTACACATTCAGGAGGCACAAACTT

GTTCCTCACTGCTGTTCACGAGATTGGCCATTCCTTAGGTCTTGGCCATTCT

AGTGATCCAAAGGCTGTAATGTTCCCCACCTACAAATATGTCGACATCAA

CACATTTCGCCTCTCTGCTGATGACATACGTGGCATTCAGTCCCTGTATGG

AGACCCAAAAGAGAACCAACGCTTGCCAAATCCTGACAATTCAGAACCAG 95238.doc •25· 1327645

CTCTCTGTGACCCCAATTTGAGTTTTGATGCTGTCACTACCGTGGGAAATA AGATCTTTTTCTTCAAAGACAGGTTCTTCTGGCTGAAGGTTTCTGAGAGAC CAAAGACCAGTGTTAATTTAATTTCTTCCTTATGGCCAACCTTGCCATCTG GCATTGAAGCTGCTTATGAAATTGAAGCCAGAAATCAAGTTTTTCTTTTTA AAGATGACAAATACTGGTTAATTAGCAATTTAAGACCAGAGCCAAATTAT CCCAAGAGCATACATTCTTTTGGTTTTCCTAACTTTGTGAAAAAAATTGAT GCAGCTGTTTTTAACCCACGTTTTTATAGGACCTACTTCTTTGTAGATAAC CAGTATTGGAGGTATGATGAAAGGAGACAGATGATGGACCCTGGTTATCC CAAACTGATTACCAAGAACTTCCAAGGAATCGGGCCTAAAATTGATGCAG TCTTCTATTCTAAAAACAAATACTACTATTTCTTCCAAGGATCTAACCAAT TTGAATATGACTTCCTACTCCAACGTATCACCAAAACACTGAAAAGCAAT AGCTGGTTTGGTTGTTAGAAATGGTGTAATTAATGGTTTTTGTTAGTTCAC TTCAGCTTAATAAGTATTTATTGCATATTTGCTATGTCCTCAGTGTACCACT ACTTAGAGATATGTATCATAAAAATAAAATCTGTAAACCATAGGTAATGA TTATATAAAATACATAATATTTTTCAATTTTGAAAACTCTAATTGTCCATTC TTGCTTGACTCTACTATTAAGTTTGAAAATAGTTACCTTCAAAGCAAGATA ATTCTATTTGAAGCATGCTCTGTAAGTTGCTTCCTAACATCCTTGGACTGA GAAATTATACTTACTTCTGGCATAACTAAAATTAAGTATATATATTTTGGC TCAAATAAAATTG 67 inhibin Serial No. βΑ > gi I 4504698 I ref NM_002192.1 | Human Inhibin, βΑ (activin A, activin ΑΒα polypeptide) (INHBA), mRNA I qPCR is determined in the context of the previous test and is compatible with [457..481]

TCCACACACACAAAAAACCTGCGCGTGAGGGGGGAGGAAAAGCAG

GGCCTTTAAAAAGGCAATCACAACAACTTTTGCTGCCAGGATGCCCTTGCT

TTGGCTGAGAGGATTTCTGTTGGCAAGTTGCTGGATTATAGTGAGGAGTTC

CCCCACCCCAGGATCCGAGGGGCACAGCGCGGCCCCCGACTGTCCGTCCT

GTGCGCTGGCCGCCCTCCCAAAGGATGTACCCAACTCTCAGCCAGAGATG

GTGGAGGCCGTCAAGAAGCACATTTTAAACATGCTGCACTTGAAGAAGAG

ACCCGATGTCACCCAGCCGGTACCCAAGGCGGCGCTTCTGAACGCGATCA

GAAAGCTTCATGTGGGCAAAGTCGGGGAGAACGGGTATGTGGAGATAGA

GGATGACATTGGAAGGAGGGCAGAAATGAATGAACTTATGGAGCAGACC

TCGGAGATCATCACGTTTGCCGAGTCAGGAACAGCCAGGAAGACGCTGCA

CTTCGAGATTTCCAAGGAAGGCAGTGACCTGTCAGTGGTGGAGCGTGCAG

AAGTCTGGCTCTTCCTAAAAGTCCCCAAGGCCAACAGGACCAGGACCAAA

GTCACCATCCGCCTCTTCCAGCAGCAGAAGCACCCGCAGGGCAGCTTGGA

CACAGGGGAAGAGGCCGAGGAAGTGGGCTTAAAGGGGGAGAGGAGTGA

ACTGTTGCTCTCTGAAAAAGTAGTAGACGCTCGGAAGAGCACCTGGCATG

TCTTCCCTGTCTCCAGCAGCATCCAGCGGTTGCTGGACCAGGGCAAGAGC 95238.doc •26· 1327645

TCCCTGGACGTTCGGATTGCCTGTGAGCAGTGCCAGGAGAGTGGCGCCAG

CTTGGTTCTCCTGGGCAAGAAGAAGAAGAAAGAAGAGGAGGGGGAAGGG

AAAAAGAAGGGCGGAGGTGAAGGTGGGGCAGGAGCAGATGAGGAAAAG

GAGCAGTCGCACAGACCTTTCCTCATGCTGCAGGCCCGGCAGTCTGAAGA

CCACCCTCATCGCCGGCGTCGGCGGGGCTTGGAGTGTGATGGCAAGGTCA

ACATCTGCTGTAAGAAACAGTTCTTTGTCAGTTTCAAGGACATCGGCTGGA

ATGACTGGATCATTGCTCCCTCTGGCTATCATGCCAACTACTGCGAGGGTG

AGTGCCCGAGCCATATAGCAGGCACGTCCGGGTCCTCACTGTCCTTCCACT

CAACAGTCATCAACCACTACCGCATGCGGGGCCATAGCCCCTTTGCCAAC

CTCAAATCGTGCTGTGTGCCCACCAAGCTGAGACCCATGTCCATGTTGTAC

TATGATGATGGTCAAAACATCATCAAAAAGGACATTCAGAACATGATCGT

GGAGGAGTGTGGGTGCTCATAGAGTTGCCCAGCCCAGGGGGAAAGGGAG

CAAGAGTTGTCCAGAGAAGACAGTGGCAAAATGAAGAAATTTTTAAGGTT

TCTGAGTTAACCAGAAAAATAGAAATTAAAAACAAAACAAAACAAAAAA

AAAAACAAAAAAAAACAAAAGTAAATTAAAAACAAACCTGATGAAACAG

ATGAAACAGATGAAGGAAGATGTGGAAATCTTAGCCTGCCTTAGCCAGGG

CTCAGAGATGAAGCAGTGAAGAGACAGATTGGGAGGGAAAGGGAGAATG

GTGTACCCTTTATTTCTTCTGAAATCACACTGATGACATCAGTTGTTTAAA

CGGGGTATTGTCCTTTCCCCCCTTGAGGTTCCCTTGTGAGCTTGAATCAAC

CAATCTGATCTGCAGTAGTGTGGACTAGAACAACCCAAATAGCATCTAGA

AAGCCATGAGTTTGAAAGGGCCCATCACAGGCACTTTCCTAGCCTAAT Sequence No. 68 Insulin-like Growth Factor 7 >gi | 4504618 | ref丨NM_001553.1丨 Human Insulin Growth Factor 7 (1〇?3卩7), 1111?^八丨9?€11 Advance_Introduction and [470..487]matching | qPCR reverse _ primer matching [567..546] | qPCR probe matching [492·.517]

GCCGCTGCCACCGCACCCCGCCATGGAGCGGCCGTCGCTGCGCGCC

CTGCTCCTCGGCGCCGCTGGGCTGCTGCTCCTGCTCCTGCCCCTCTCCTCTT

CCTCCTCTTCGGACACCTGCGGCCCCTGCGAGCCGGCCTCCTGCCCGCCCC

TGCCCCCGCTGGGCTGCCTGCTGGGCGAGACCCGCGACGCGTGCGGCTGC

TGCCCTATGTGCGCCCGCGGCGAGGGCGAGCCGTGCGGGGGTGGCGGCGC

CGGCAGGGGGTACTGCGCGCCGGGCATGGAGTGCGTGAAGAGCCGCAAG

AGGCGGAAGGGTAAAGCCGGGGCAGCAGCCGGCGGTCCGGGTGTAAGCG

GCGTGTGCGTGTGCAAGAGCCGCTACCCGGTGTGCGGCAGCGACGGCACC

ACCTACCCGAGCGGCTGCCAGCTGCGCGCCGCCAGCCAGAGGGCCGAGA

GCCGCGGGGAGAAGGCCATCACCCAGGTCAGCAAGGGCACCTGCGAGCA

AGGTCCTTCCATAGTGACGCCCCCCAAGGACATCTGGAATGTCACTGGTG 95238.doc -27- 1327645 CCCAGGTGTACTTGAGCTGTGAGGTCATCGGAATCCCGACACCTGTCCTCA TCTGGAACAAGGTAAAAAGGGGTCACTATGGAGTTCAAAGGACAGAACT CCTGCCTGGTGACCGGGACAACCTGGCCATTCAGACCCGGGGTGGCCCAG AAAAGCATGAAGTAACTGGCTGGGTGCTGGTATCTCCTCTAAGTAAGGAA GATGCTGGAGAATATGAGTGCCATGCATCCAATTCCCAAGGACAGGCTTC AGCATCAGCAAAAATTACAGTGGTTGATGCCTTACATGAAATACCAGTGA AAAAAGGTGAAGGTGCCGAGCTATAAACCTCCAGAATATTATTAGTCTGC ATGGTTAAAAGTAGTCATGGATAACTACATTACCTGTTCTTGCCTAATAAG TTTCTTTTAATCCAATCCACTAACACTTTAGTTATATTCACTGGTTTTACAC AGAGAAATACAAAATAAAGATCACACATCAAGACTATCTACAAAAATTTA TTATATATTTACAGAAGAAAAGCATGCATATCATTAAACAAATAAAATAC TTTTTATCACAAAAAAAAAAAAAAAA Serial No. 69 γ- glutamine acyl hydrolase > gi | 4503986 | ref Shu ΝΜ_003878.1 | human [gamma] Acyl hydrolysis hub amine Enzyme (ligase, phorbol gamma glutamine hydrolase) (GGH), mRNA | 9卩匸11 advance _ primer matched with [53 1..547] | qPCR reverse _ primer and [611·.587] Matching | qPCR probe matches [549..577]

TGCCGCAGCCCCCGCCCGCCCGCAGAGCTTTTGAAAGGCGGCGGG

AGGCGGCGAGCGCCATGGCCAGTCCGGGCTGCCTGCTGTGCGTGCTGGGC

CTGCTACTCTGCGGGGCGGCGAGCCTCGAGCTGTCTAGACCCCACGGCGA

CACCGCCAAGAAGCCCATCATCGGAATATTAATGCAAAAATGCCGTAATA

AAGTCATGAAAAACTATGGAAGATACTATATTGCTGCGTCCTATGTAAAG

TACTTGGAGTCTGCAGGTGCGAGAGTTGTACCAGTAAGGCTGGATCTTAC

AGAGAAAGACTATGAAATACTTTTCAAATCTATTAATGGAATCCTTTTCCC

TGGAGGAAGTGTTGACCTCAGACGCTCAGATTATGCTAAAGTGGCCAAAA

TATTTTATAACTTGTCCATACAGAGTTTTGATGATGGAGACTATTTTCCTGT

GTGGGGCACATGCCTTGGATTTGAAGAGCTTTCACTGCTGATTAGTGGAG

AGTGCTTATTAACTGCCACAGATACTGTTGACGTGGCAATGCCGCTGAACT

TCACTGGAGGTCAATTGCACAGCAGAATGTTCCAGAATTTTCCTACTGAGT

TGTTGCTGTCATTAGCAGTAGAACCTCTGACTGCCAATTTCCATAAGTGGA

GCCTCTCCGTGAAGAATTTTACAATGAATGAAAAGTTAAAGAAGTTTTTC

AATGTCTTAACTACAAATACAGATGGCAAGATTGAGTTTATTTCAACAAT

GGAAGGATATAAGTATCCAGTATATGGTGTCCAGTGGCATCCAGAGAAAG

CACCTTATGAGTGGAAGAATTTGGATGGCATTTCCCATGCACCTAATGCTG

TGAAAACCGCATTTTATTTAGCAGAGTTTTTTGTTAATGAAGCTCGGAAAA

ACAACCATCATTTTAAATCTGAATCTGAAGAGGAGAAAGCATTGATTTAT

CAGTTCAGTCCAATTTATACTGGAAATATTTCTTCATTTCAGCAATGTTAC

ATATTTGATTGAAAGTCTTCAATTTGTTAACAGAGCAAATTTGAATAATTC 95238.doc -28- 1327645 CATGATTAAACTGTTAGAATAACTTGCTACTCATGGCAAGATTAGGAAGT CACAGATTCTTTTCTATAATGTGCCTGGCTCTGATTCTTCATTATGTATGTG ACTATTTATATAACATTAGATAATTAAATAGTGAGACATAAATAGAGTGC TTTTTCATGGAAAAGCCTTCTTATATCTGAAGATTGAAAAATAAATTTACT GAAATACAAAAAAAAAAAAAAA serial No. 70 leucine-rich proteoglycan of proline 1 > gi | 21361917 | ref | NM_02235 6.2 | human leucine proline-rich of Proteoglycan (Lipson), (LEPRE1), mRNA | qPCR Advance - Primer matches [813..836] | qPCR reverse _ primer matches [894..872] | qPCR probe and [841·.870] match

GGTGGCGGGTGGCTGGCGGTTCCGTTAGGTCTGAGGGAGCGATGG

CGGTACGCGCGTTGAAGCTGCTGACCACACTGCTGGCTGTCGTGGCCGCT

GCCTCCCAAGCCGAGGTCGAGTCCGAGGCAGGATGGGGCATGGTGACGCC

TGATCTGCTCTTCGCCGAGGGGACCGCAGCCTACGCGCGCGGGGACTGGC

CCGGGGTGGTCCTGAGCATGGAACGGGCGCTGCGCTCCCGGGCAGCCCTC

CGCGCCCTTCGCCTGCGCTGCCGCACCCAGTGTGCCGCCGACTTCCCGTGG

GAGCTGGACCCCGACTGGTCCCCCAGCCCGGCCCAGGCCTCGGGCGCCGC

CGCCCTGCGCGACCTGAGCTTCTTCGGGGGCCTTCTGCGTCGCGCTGCCTG

CCTGCGCCGCTGCCTCGGGCCGCCGGCCGCCCACTCGCTCAGCGAAGAGA

TGGAGCTGGAGTTCCGCAAGCGGAGCCCCTACAACTACCTGCAGGTCGCC

TACTTCAAGATCAACAAGTTGGAGAAAGCTGTTGCTGCAGCACACACCTT

CTTCGTGGGCAATCCTGAGCACATGGAAATGCAGCAGAACCTAGACTATT

ACCAAACCATGTCTGGAGTGAAGGAGGCCGACTTCAAGGATCTTGAGACT

CAACCCCATATGCAAGAATTTCGACTGGGAGTGCGACTCTACTCAGAGGA

ACAGCCACAGGAAGCTGTGCCCCACCTAGAGGCGGCGCTGCAAGAATACT

TTGTGGCCTATGAGGAGTGCCGTGCCCTCTGCGAAGGGCCCTATGACTAC

GATGGCTACAACTACCTTGAGTACAACGCTGACCTCTTCCAGGCCATCAC

AGATCATTACATCCAGGTCCTCAACTGTAAGCAGAACTGTGTCACGGAGC

TTGCTTCCCACCCAAGTCGAGAGAAGCCCTTTGAAGACTTCCTCCCATCGC

ATTATAATTATCTGCAGTTTGCCTACTATAACATTGGGAATTATACACAGG

CTGTTGAATGTGCCAAGACCTATCTTCTCTTCTTCCCCAATGACGAGGTGA

TGAACCAAAATTTGGCCTATTATGCAGCTATGCTTGGAGAAGAACACACC

AGATCCATCGGCCCCCGTGAGAGTGCCAAGGAGTACCGACAGCGAAGCCT

ACTGGAAAAAGAACTGCTTTTCTTCGCTTATGATGTTTTTGGAATTCCCTTT

GTGGATCCGGATTCATGGACTCCAGGAGAAGTGATTCCCAAGAGATTGCA

AGAGAAACAGAAGTCAGAACGGGAAACAGCCGTACGCATCTCCCAGGAG

ATTGGGAACCTTATGAAGGAAATCGAGACCCTTGTGGAAGAGAAGACCA

AGGAGTCACTGGATGTGAGCAGACTGACCCGGGAAGGTGGCCCCCTGCTG 95238.doc -29· 1327645

TATGAAGGCATCAGTCTCACCATGAACTCCAAACTCCTGAATGGTTCCCA

GCGGGTGGTGATGGACGGCGTAATCTCTGACCACGAGTGTCAGGAGCTGC

AGAGACTGACCAATGTGGCAGCAACCTCAGGAGATGGCTACCGGGGTCA

GACCTCCCCACATACTCCCAATGAAAAGTTCTATGGTGTCACTGTCTTCAA

AGCCCTCAAGCTGGGGCAAGAAGGCAAAGTTCCTCTGCAGAGTGCCCACC

TGTACTACAACGTGACGGAGAAGGTGCGGCGCATCATGGAGTCCTACTTC

CGCCTGGATACGCCCCTCTACTTTTCCTACTCTCATCTGGTGTGCCGCACT

GCCATCGAAGAGGTCCAGGCAGAGAGGAAGGATGATAGTCATCCAGTCC

ACGTGGACAACTGCATCCTGAATGCCGAGACCCTCGTGTGTGTCAAAGAG

CCCCCAGCCTACACCTTCCGCGACTACAGCGCCATCCTTTACCTAAATGGG

GACTTCGATGGCGGAAACTTTTATTTCACTGAACTGGATGCCAAGACCGT

GACGGCAGAGGTGCAGCCTCAGTGTGGAAGAGCCGTGGGATTCTCTTCAG

GCACTGAAAACCCACATGGAGTGAAGGCTGTCACCAGGGGGCAGCGCTGT

GCCATCGCCCTGTGGTTCACCCTGGACCCTCGACACAGCGAGCGGGTGAG

AGCAGCTCGAGCGGGTGAGAGCAGCTGGTGCTGTGGTGACCCGTTCCCAG

AGCGCCCTTGGTTTGCCTTTCTCTTCCCCAAATCCCATTGCCAGTGGCTGA

GACACGAAAGGAGCACTTGGGACACCAGCTCCAACGCCCTGTCATTATGG

TCACATTGCCTTGTCCTCCCTGGGCCTGCTGTGAACGGGATCCAGGTGGGG

AAAGAGGTCAAGACAGGGAGCGATGCTGAGTTCTTGGTTCCCTCCTTGGG

CCCCACTTCAGCTGTCCTTTTCCAGAGAGTAGGACCTGCTGGGAAGGAGA

TGAGCCTGGGGCCATTAAGGAACCTTCCTTGTCCCCTGGGAAGTAGCAGC

TGAGAGATAGCGAGTGTCTGGAGCGGAGGCCTCTCTGAATGGGCAGGGGT

TTGTCCTTGCAGGACAGGGTGCAGGCAGATGACCTGGTGAAGATGCTCTT

CAGCCCAGAAGAGATGGTCCTCTCCCAGGAGCAGCCCCTGGATGCCCAGC

AGGGCCCCCCCGAACCTGCACAAGAGTCTCTCTCAGAGAGTGAATCGAAG

CCCAAGGATGAGCTATGACAGCGTCCAGGTCAGACGGATGGGTGACTAGA

CCCATGGAGAGGAACTCTTCTGCACTCTGAGCTGGCCAGCCCCTCGGGGC

TGCAGAGCAGTGAGCCTACATCTGCCACTCAGCCGAGGGGACCCTGCTCA

CAGCCTTCTACATGGTGCTACTGCTCTTGGAGTGGACATGACCAGACACC

GCACCCCCTGGATCTGGCTGAGGGCTCAGGACACAGGCCCAGCCACCCCC

AGGGGCCTCCACAGGCCGCTGCATAACAGCGATACAGTACTTAAGTGTCT

GTGTAGACAACCAAAGAATAAATGATTCATGGTTTTTTTT Sequence No. 71

Cysteine protease inhibitor S >gi | 19882254丨ref | NM_001899.2丨 Human cysteine protease inhibitor S (CST4), mRNA | qPCR advance _ primer matched with [343..361] | qPCR reverse - The primer matches [434..411] | The qPCR probe matches [382·.410] 95238.doc -30- 1327645

GGCTCTCACCCTCCTCTCCTGCAGCTCCAGCTTTGTGCTCTGCCTCT GAGGAGACCATGGCCCGGCCTCTGTGTACCCTGCTACTCCTGATGGCTACC CTGGCTGGGGCTCTGGCCTCGAGCTCCAAGGAGGAGAATAGGATAATCCC AGGTGGCATCTATGATGCAGACCTCAATGATGAGTGGGTACAGCGTGCCC TTCACTTCGCCATCAGCGAGTACAACAAGGCCACCGAAGATGAGTACTAC AGACGCCCGCTGCAGGTGCTGCGAGCCAGGGAGCAGACCTTTGGGGGGGT GAATTACTTCTTCGACGTAGAGGTGGGCCGCACCATATGTACCAAGTCCC AGCCCAACTTGGACACCTGTGCCTTCCATGAACAGCCAGAACTGCAGAAG AAACAGTTGTGCTCTTTCGAGATCTACGAAGTTCCCTGGGAGGACAGAAT GTCCCTGGTGAATTCCAGGTGTCAAGAAGCCTAGGGGTCTGTGCCAGGCC AGTCACACCGACCACCACCCACTCCCACCCACTGTAGTGCTCCCACCCCTG GACTGGTGGCCCCCACCCTGCGGGAGGCCTCCCCATGTGCCTGTGCCAAG AGACAGACAGAGAAGGCTGCAGGAGTCCTTTGTTGCTCAGCAGGGCGCTC TGCCCTCCCTCCTTCCTTCTTGCTTCTAATAGACCTGGTACATGGTACACAC ACCCCCACCTCCTGCAATTAAACAGTAGCATCGCC Serial No. 72 secreted frizzled related protein 4 >. Gi | 8400733 | ref | NM_003014.2 | human secreted frizzled related protein 4 (SFRP4), mRNA | qPCR is matched with [1079·.1103] when requested in the context.

GGCGGGTTCGCGCCCCGAAGGCTGAGAGCTGGCGCTGCTCGTGCCC

TGTGTGCCAGACGGCGGAGCTCCGCGGCCGGACCCCGCGGCCCCGCTTTG

CTGCCGACTGGAGTTTGGGGGAAGAAACTCTCCTGCGCCCCAGAAGATTT

CTTCCTCGGCGAAGGGACAGCGAAAGATGAGGGTGGCAGGAAGAGAAGG

CGCTTTCTGTCTGCCGGGGTCGCAGCGCGAGAGGGCAGTGCCATGTTCCTC

TCCATCCTAGTGGCGCTGTGCCTGTGGCTGCACCTGGCGCTGGGCGTGCGC

GGCGCGCCCTGCGAGGCGGTGCGCATCCCTATGTGCCGGCACATGCCCTG

GAACATCACGCGGATGCCCAACCACCTGCACCACAGCACGCAGGAGAAC

GCCATCCTGGCCATCGAGCAGTACGAGGAGCTGGTGGACGTGAACTGCAG

CGCCGTGCTGCGCTTCTTCTTCTGTGCCATGTACGCGCCCATTTGCACCCT

GGAGTTCCTGCACGACCCTATCAAGCCGTGCAAGTCGGTGTGCCAACGCG

CGCGCGACGACTGCGAGCCCCTCATGAAGATGTACAACCACAGCTGGCCC

GAAAGCCTGGCCTGCGACGAGCTGCCTGTCTATGACCGTGGCGTGTGCAT

TTCGCCTGAAGCCATCGTCACGGACCTCCCGGAGGATGTTAAGTGGATAG

ACATCACACCAGACATGATGGTACAGGAAAGGCCTCTTGATGTTGACTGT

AAACGCCTAAGCCCCGATCGGTGCAAGTGTAAAAAGGTGAAGCCAACTTT

GGCAACGTATCTCAGCAAAAACTACAGCTATGTTATTCATGCCAAAATAA

AAGCTGTGCAGAGGAGTGGCTGCAATGAGGTCACAACGGTGGTGGATGTA

AAAGAGATCTTCAAGTCCTCATCACCCATCCCTCGAACTCAAGTCCCGCTC

ATTACAAATTCTTCTTGCCAGTGTCCACACATCCTGCCCCATCAAGATGTT 95238.doc -31- 1327645

CTCATCATGTGTTACGAGTGGCGTTCAAGGATGATGCTTCTTGAAAATTGC TTAGTTGAAAAATGGAGAGATCAGCTTAGTAAAAGATCCATACAGTGGGA AGAGAGGCTGCAGGAACAGCGGAGAACAGTTCAGGACAAGAAGAAAACA GCCGGGCGCACCAGTCGTAGTAATCCCCCCAAACCAAAGGGAAAGCCTCC TGCTCCCAAACCAGCCAGTCCCAAGAAGAACATTAAAACTAGGAGTGCCC AGAAGAGAACAAACCCGAAAAGAGTGTGAGCTAACTAGTTTCCAAAGCG GAGACTTCCGACTTCCTTACAGGATGAGGCTGGGCATTGCCTGGGACAGC CTATGTAAGGCCATGTGCCCCTTGCCCTAACAACTCACTGCAGTGCTCTTC ATAGACACATCTTGCAGCATTTTTCTTAAGGCTATGCTTCAGTTTTTCTTTG TAAGCCATCACAAGCCATAGTGGTAGGTTTGCCCTTTGGTACAGAAGGTG AGTTAAAGCTGGTGGAAAAGGCTTATTGCATTGCATTCAGAGTAACCTGT GTGCATACTCTAGAAGAGTAGGGAAAATAATGCTTGTTACAATTCGACCT AATATGTGCATTGTAAAATAAATGCCATATTTCAAACAAAACACGTAATT TTTTTACAGTATGTTTTATTACCTTTTGATATCTGTTGTTGCAATGTTAGTG ATGTTTTAAAATGTGATGAAAATATAATGTTTTTAAGAAGGAACAGTAGT GGAATGAATGTTAAAAGATCTTTATGTGTTTATGGTCTGCAGAAGGATTTT TGTGATGAAAGGGGATTTTTTGAAAAATTAGAGAAGTAGCATATGGAAAA TTATAATGTGTTTTTTTACCAATGACTTCAGTTTCTGTTTTTAGCTAGAAAC TTAAAAACAAAAATAATAATAAAGAAAAATAAATAAAAAGGAGAGGCAG ACAATGTCTGGATTCCTGTTTTTTG GTTACCTGATTTCCATGATCATGATGC TTCTTGTCAACACCCTCTTAAGCAGCACCAGAAACAGTGAGTTTGTCTGTA CCATTAGGAGTTAGGTACTAATTAGTTGGCTAATGCTCAAGTATTTTATAC CCACAAGAGAGGTATGTCACTCATCTTACTTCCCAGGACATCCACCCTGA GAATAATTTGACAAGCTTAAAAATGGCCTTCATGTGAGTGCCAAATTTTGT TTTTCTTCATTTAAATATTTTCTTTGCCTAAATACATGTGAGAGGAGTTAA ATATAAATGTACAGAGAGGAAAGTTGAGTTCCACCTCTGAAATGAGAATT ACTTGACAGTTGGGATACTTTAATCAGAAAAAAAGAACTTATTTGCAGCA TTTTATCAACAAATTTCATAATTGTGGACAATTGGAGGCATTTATTTTAAA AAACAATTTTATTGGCCTTTTGCTAACACAGTAAGCATGTATTTTATAAGG CATTCAATAAATGCACAACGCCCAAAGGAAATAAAATCCTATCTAATCCT ACTCTCCACTACACAGAGGTAATCACTATTAGTATTTTGGCATATTATTCT CCAGGTGTTTGCTTATGCACTTATAAAATGATTTGAACAAATAAAACTAG GAACCTGTATACATGTGTTTCATAACCTGCCTCCTTTGCTTGGCCCTTTATT GAGATAAGTTTTCCTGTCAAGAAAGCAGAAACCATCTCATTTCTAACAGC TGTGTTATATTCCATAGTATGCATTACTCAACAAACTGTTGTGCTATTGGA TACTTAGGTGGTTTCTTCACTGACAATACTGAATAAACATCTCACCGGAAT TC Serial No. 73 Asi Bo Yun > gi I 41350213 | ref | NM_017680.3 | human Asi Bo Yun (LRR type) (ASPN), mRNA |qPCR advancement - introduction and [798 ..823] phase 95238.doc -32· 1327645 with I qPCR reverse-introduction matched with [934..912] 丨qPCR probe matched with [842..875]

AGTACTAACATGGACTAATCTGTGGGAGCAGTTTATTCCAGTATCA

CCCAGGGTGCAGCCACACCAGGACTGTGTTGAAGGGTGTTTTTTTTCTTTT

AAATGTAATACCTCCTCATCTTTTCTTCTTACACAGTGTCTGAGAACATTT

ACATTATAGATAAGTAGTACATGGTGGATAACTTCTACTTTTAGGAGGACT

ACTCTCTTCTGACAGTCCTAGACTGGTCTTCTACACTAAGACACCATGAAG

GAGTATGTGCTCCTATTATTCCTGGCTTTGTGCTCTGCCAAACCCTTCTTTA

GCCCTTCACACATCGCACTGAAGAATATGATGCTGAAGGATATGGAAGAC

ACAGATGATGATGATGATGATGATGATGATGATGATGATGATGATGAGGA

CAACTCTCTTTTTCCAACAAGAGAGCCAAGAAGCCATTTTTTTCCATTTGA

TCTGTTTCCAATGTGTCCATTTGGATGTCAGTGCTATTCACGAGTTGTACA

TTGCTCAGATTTAGGTTTGACCTCAGTCCCAACCAACATTCCATTTGATAC

TCGAATGCTTGATCTTCAAAACAATAAAATTAAGGAAATCAAAGAAAATG

ATTTTAAAGGACTCACTTCACTTTATGGTCTGATCCTGAACAACAACAAGC

TAACGAAGATTCACCCAAAAGCCTTTCTAACCACAAAGAAGTTGCGAAGG

CTGTATCTGTCCCACAATCAACTAAGTGAAATACCACTTAATCTTCCCAAA

TCATTAGCAGAACTCAGAATTCATGAAAATAAAGTTAAGAAAATACAAAA

GGACACATTCAAAGGAATGAATGCTTTACACGTTTTGGAAATGAGTGCAA

ACCCTCTTGATAATAATGGGATAGAGCCAGGGGCATTTGAAGGGGTGACG

GTGTTCCATATCAGAATTGCAGAAGCAAAACTGACCTCAGTTCCTAAAGG

CTTACCACCAACTTTATTGGAGCTTCACTTAGATTATAATAAAATTTCAAC

AGTGGAACTTGAGGATTTTAAACGATACAAAGAACTACAAAGGCTGGGCC

TAGGAAACAACAAAATCACAGATATCGAAAATGGGAGTCTTGCTAACATA

CCACGTGTGAGAGAAATACATTTGGAAAACAATAAACTAAAAAAAATCCC

TTCAGGATTACCAGAGTTGAAATACCTCCAGATAATCTTCCTTCATTCTAA

TTCAATTGCAAGAGTGGGAGTAAATGACTTCTGTCCAACAGTGCCAAAGA

TGAAGAAATCTTTATACAGTGCAATAAGTTTATTCAACAACCCGGTGAAA

TACTGGGAAATGCAACCTGCAACATTTCGTTGTGTTTTGAGCAGAATGAGT

GTTCAGCTTGGGAACTTTGGAATGTAATAATTAGTAATTGGTAATGTCCAT

TTAATATAAGATTCAAAAATCCCTACATTTGGAATACTTGAACTCTATTAA

TAATGGTAGTATTATATATACAAGCAAATATCTATTCTCAAGTGGTAAGTC

CACTGACTTATTTTATGACAAGAAATTTCAACGGAATTTTGCCAAACTATT

GATACATAAGGGTTGAGAGAAACAAGCATCTATTGCAGTTTCTTTTTGCGT

ACAAATGATCTTACATAAATCTCATGCTTGACCATTCCTTTCTTCATAACA

AAAAAGTAAGATATTCGGTATTTAACACTTTGTTATCAAGCATATTTTAAA

AAGAACTGTACTGTAAATGGAATGCTTGACTTAGCAAAATTTGTGCTCTTT

CATTTGCTGTTAGAAAAACAGAATTAACAAAGACAGTAATGTGAAGAGTG

CATTACACTATTCTTATTCTTTAGTAACTTGGGTAGTACTGTAATATTTTTA

ATCATCTTAAAGTATGATTTGATATAATCTTATTGAAATTACCTTATCATG

TCTTAGAGCCCGTCTTTATGTTTAAAACTAATTTCTTAAAATAAAGCCTTC 95238.doc -33- 1327645

AGTAAATGTTCATTACCAACTTGATAAATGCTACTCATAAGAGCTGGTTTG

GGGCTATAGCATATGCTTTTTTTTTTTTAATTATTACCTGATTTAAAAATCT

CTGTAAAAACGTGTAGTGTTTCATAAAATCTGTAACTCGCATTTTAATGAT

CCGCTATTATAAGCTTTTAATAGCATGAAAATTGTTAGGCTATATAACATT

GCCACTTCAACTCTAAGGAATATTTTTGAGATATCCCTTTGGAAGACCTTG

CTTGGAAGAGCCTGGACACTAACAATTCTACACCAAATTGTCTCTTCAAAT

ACGTATGGACTGGATAACTCTGAGAAACACATCTAGTATAACTGAATAAG

CAGAGCATCAAATTAAACAGACAGAAACCGAAAGCTCTATATAAATGCTC

AGAGTTCTTTATGTATTTCTTATTGGCATTCAACATATGTAAAATCAGAAA

ACAGGGAAATTTTCATTAAAAATATTGGTTTGAAATAAAAAAAAAAAAAA Sequence No. 74 Cell growth regulator with EF hand function site 1

>gi | 33589823 | ref | NM_006569.2 | Human cell growth regulator (CGREF1) with EF hand function site 1 , mRNA 丨qPCR advance _ primer matched with [378..394] | qPCR reverse - primer and [455 ..431] Matching 丨4?011 probe matches [396..415]

CGCGCAGCCCCTCCGGCCGCGGGCGCAGCGGGGGCGCTGGTGGAG

CTGCGAAGGGCCAGGTCCGGCGGGCGGGGCGGCGGCTGGCACTGGCTCC

GGACTCTGCCCGGCCAGGGCGGCGGCTCCAGCCGGGAGGGCGACGTGGA

GCGGCCACGTGGAGCGGCCCGGGGGAGGCTGGCGGCGGGAGGCGAGGCG

CGGGCGGCGCAGCAGCCAGGAGCGCCCACGGAGCTGGACCCCCAGAGCC

GCGCGGCGCCGCAGCAGTTCCAGGAAGGATGTTACCTTTGACGATGACAG

TGTTAATCCTGCTGCTGCTCCCCACGGGTCAGGCTGCCCCAAAGGATGGA

GTCACAAGGCCAGACTCTGAAGTGCAGCATCAGCTCCTGCCCAACCCCTT

CCAGCCAGGCCAGGAGCAGCTCGGACTTCTGCAGAGCTACCTAAAGGGAC

TAGGAAGGACAGAAGTGCAACTGGAGCATCTGAGCCGGGAGCAGGTTCT

CCTCTACCTCTTTGCCCTCCATGACTATGACCAGAGTGGACAGCTGGATGG

CCTGGAGCTGCTGTCCATGTTGACAGCTGCTCTGGCCCCTGGAGCTGCCAA

CTCTCCTACCACCAACCCGGTGATATTGATAGTGGACAAAGTGCTCGAGA

CGCAGGACCTGAATGGGGATGGGCTCATGACCCCTGCTGAGCTCATCAAC

TTCCCGGGAGTAGCCCTCAGGCACGTGGAGCCCGGAGAGCCCCTTGCTCC

ATCTCCTCAGGAGCCACAAGCTGTTGGAAGGCAGTCCCTATTAGCTAAAA

GCCCATTAAGACAAGAAACACAGGAAGCCCCTGGTCCCAGAGAAGAAGC

AAAGGGCCAGGTAGAGGCCAGAAGGGAGTCTTTGGATCCTGTCCAGGAG

CCTGGGGGCCAGGCAGAGGCTGATGGAGATGTTCCAGGGCCCAGAGGGG

AAGCTGAGGGCCAGGCAGAGGCTAAAGGAGATGCCCCTGGGCCCAGAGG

GGAAGCTGGGGGCCAGGCAGAGGCTGAAGGAGATGCCCCCGGGCCCAGA

GGGGAAGCTGGGGGCCAGGCAGAGGCCAGGGAGAATGGAGAGGAGGCC 95238.doc -34- 1327645

AAGGAACTTCCAGGGGAAACACTGGAGTCTAAGAACACCCAAAATGACTT TGAGGTGCACATTGTTCAAGTGGAGAATGATGAGATCTAGATCTTGAAGA TACAGGTACCCCACGAAGTCTCAGTGCCAGAACATAAGCCCTGAAGTGGG CAGGGGAAATGTACGCTGGGACAAGGACCATCTCTGTGCCCCCTGTCTGG TCCCAGTAGGTATCAGGTCTTTCTGTGCAGCTCAGGGAGACCCTAAGTTAA GGGGCAGATTACCAATAAAGAACTGAATGAATTCATCCCCCCGGGCCACC TCTCTACCCGTCCAGCCTGCCCAGACCCTCTCAGAGGAACGGGGTTGGGG ACCGAAAGGACAGGGATGCCGCCTGCCCAGTGTTTCTGGGCCTCACGGTG CTCCGGCAGCAGAGCGCATGGTGCTAGCCATGGCCGGCTGCAGAGGACCC AGTGAGGAAAGCTCAGTCTATCCCTGGGCCCCAAACCCTCACCGGTTCCC CCTCACCTGGTGTTCAGACACCCCATGCTCTCCTGCAGCTCAGGGCAGGTG ACCCCATCCCCAGTAATATTAATCATCACTAGAACTTTTTGAGAGCCTTGT ACACATCAGGCATCATGCTGGGCATTTTATATATGATTTTATCCTCACAAT AATTCTGTAGCCAAGCAGAATTGGTTCCATTTGACAGATGAAGAAATTGA GGCAGATTGCGTTAAGTGCTGTACCCTAAGGTGATATGCAGCTAATTAAA TGGC AG ATTTGA AAAAAAA AA A AA AAAAA AAAA AA AA AA AA AAAA AAA serial No. 75 kallikrein 10, transcript variant 1 > gi | 22208981 Shu Ref | NM—002776.3丨 Human kallikrein 10 (KLK10), transcript variant 1, mRNA before PCRqPCR _ Introduction and [851..874] match |? 4 € 11 reverse _ Introduction and [950..931] match |? 4 square feet probes [890..914] match

CATCCTGCCACCCCTAGCCTTGCTGGGGACGTGAACCCTCTCCCCG

CGCCTGGGAAGCCTTCTTGGCACCGGGACCCGGAGAATCCCCACGGAAGC

CAGTTCCAAAAGGGATGAAAAGGGGGCGTTTCGGGCACTGGGAGAAGCC

TGTATTCCAGGGCCCCTCCCAGAGCAGGAATCTGGGACCCAGGAGTGCCA

GCCTCACCCACGCAGATCCTGGCCATGAGAGCTCCGCACCTCCACCTCTCC

GCCGCCTCTGGCGCCCGGGCTCTGGCGAAGCTGCTGCCGCTGCTGATGGC

GCAACTCTGGGCCGCAGAGGCGGCGCTGCTCCCCCAAAACGACACGCGCT

TGGACCCCGAAGCCTATGGCTCCCCGTGCGCGCGCGGCTCGCAGCCCTGG

CAGGTCTCGCTCTTCAACGGCCTCTCGTTCCACTGCGCGGGTGTCCTGGTG

GACCAGAGTTGGGTGCTGACGGCCGCGCACTGCGGAAACAAGCCACTGTG

GGCTCGAGTAGGGGATGACCACCTGCTGCTTCTTCAGGGAGAGCAGCTCC

GCCGGACCACTCGCTCTGTTGTCCATCCCAAGTACCACCAGGGCTCAGGC

CCCATCCTGCCAAGGCGAACGGATGAGCACGATCTCATGTTGCTGAAGCT

GGCCAGGCCCGTAGTGCTGGGGCCCCGCGTCCGGGCCCTGCAGCTTCCCT

ACCGCTGTGCTCAGCCCGGAGACCAGTGCCAGGTTGCTGGCTGGGGCACC

ACGGCCGCCCGGAGAGTGAAGTACAACAAGGGCCTGACCTGCTCCAGCAT 95238.doc -35- 1327645

CACTATCCTGAGCCCTAAAGAGTGTGAGGTCTTCTACCCTGGCGTGGTCAC CAACAACATGATATGTGCTGGACTGGACCGGGGCCAGGACCCTTGCCAGA GTGACTCTGGAGGCCCCCTGGTCTGTGACGAGACCCTCCAAGGCATCCTCT CGTGGGGTGTTTACCCCTGTGGCTCTGCCCAGCATCCAGCTGTCTACACCC AGATCTGCAAATACATGTCCTGGATCAATAAAGTCATACGCTCCAACTGA TCCAGATGCTACGCTCCAGCTGATCCAGATGTTATGCTCCTGCTGATCCAG ATGCCCAGAGGCTCCATCGTCCATCCTCTTCCTCCCCAGTCGGCTGAACTC TCCCCTTGTCTGCACTGTTCAAACCTCTGCCGCCCTCCACACCTCTAAACA TCTCCCCTCTCACCTCATTCCCCCACCTATCCCCATTCTCTGCCTGTACTGA AGCTGAAATGCAGGAAGTGGTGGCAAAGGTTTATTCCAGAGAAGCCAGG AAGCCGGTCATCACCCAGCCTCTGAGAGCAGTTACTGGGGTCACCCAACC TGACTTCCTCTGCCACTCCCTGCTGTGTGACTTTGGGCAAGCCAAGTGCCC TCTCTGAACCTCAGTTTCCTCATCTGCAAAATGGGAACAATGACGTGCCTA CCTCTTAGACATGTTGTGAGGAGACTATGATATAACATGTGTATGTAAATC TTCATGGTGATTGTCATGTAAGGCTTAACACAGTGGGTGGTGAGTTCTGAC TAAAGGTTACCTGTTGTCGTGA Serial No. 76 kallikrein 10, transcript variant 2 & gt body; gi | 22208983 | ref | NM_145888.1 | human kallikrein

10 (KLK10), transcript variant 2, mRNA | qPCR advancement - primer matches [714..737] | qPCR reverse-primer matches [813..794] | qPCR probe matches [753..777]

ACCAGCGGCAGACCACAGGCAGGGCAGAGGCACGTCTGGGTCCCC

TCCCTCCTTCCTATCGGCGACTCCCAGGATCCTGGCCATGAGAGCTCCGCA

CCTCCACCTCTCCGCCGCCTCTGGCGCCCGGGCTCTGGCGAAGCTGCTGCC

GCTGCTGATGGCGCAACTCTGGGCCGCAGAGGCGGCGCTGCTCCCCCAAA

ACGACACGCGCTTGGACCCCGAAGCCTATGGCTCCCCGTGCGCGCGCGGC

TCGCAGCCCTGGCAGGTCTCGCTCTTCAACGGCCTCTCGTTCCACTGCGCG

GGTGTCCTGGTGGACCAGAGTTGGGTGCTGACGGCCGCGCACTGCGGAAA

CAAGCCACTGTGGGCTCGAGTAGGGGATGACCACCTGCTGCTTCTTCAGG

GAGAGCAGCTCCGCCGGACCACTCGCTCTGTTGTCCATCCCAAGTACCAC

CAGGGCTCAGGCCCCATCCTGCCAAGGCGAACGGATGAGCACGATCTCAT

GTTGCTGAAGCTGGCCAGGCCCGTAGTGCTGGGGCCCCGCGTCCGGGCCC

TGCAGCTTCCCTACCGCTGTGCTCAGCCCGGAGACCAGTGCCAGGTTGCTG

GCTGGGGCACCACGGCCGCCCGGAGAGTGAAGTACAACAAGGGCCTGAC

CTGCTCCAGCATCACTATCCTGAGCCCTAAAGAGTGTGAGGTCTTCTACCC

TGGCGTGGTCACCAACAACATGATATGTGCTGGACTGGACCGGGGCCAGG

ACCCTTGCCAGAGTGACTCTGGAGGCCCCCTGGTCTGTGACGAGACCCTC

CAAGGCATCCTCTCGTGGGGTGTTTACCCCTGTGGCTCTGCCCAGCATCCA 95238.doc -36- 1327645 GCTGTCTACACCCAGATCTGCAAATACATGTCCTGGATCAATAAAGTCAT ACGCTCCAACTGATCCAGATGCTACGCTCCAGCTGATCCAGATGTTATGCT CCTGCTGATCCAGATGCCCAGAGGCTCCATCGTCCATCCTCTTCCTCCCCA GTCGGCTGAACTCTCCCCTTGTCTGCACTGTTCAAACCTCTGCCGCCCTCC ACACCTCTAAACATCTCCCCTCTCACCTCATTCCCCCACCTATCCCCATTCT CTGCCTGTACTGAAGCTGAAATGCAGGAAGTGGTGGCAAAGGTTTATTCC AGAGAAGCCAGGAAGCCGGTCATCACCCAGCCTCTGAGAGCAGTTACTGG GGTCACCCAACCTGACTTCCTCTGCCACTCCCTGCTGTGTGACTTTGGGCA AGCCAAGTGCCCTCTCTGAACCTCAGTTTCCTCATCTGCAAAATGGGAACA ATGACGTGCCTACCTCTTAGACATGTTGTGAGGAGACTATGATATAACAT GTGTATGTAAATCTTCATGGTGATTGTCATGTAAGGCTTAACACAGTGGGT GGTGAGTTCTGACTAAAGGTTACCTGTTGTCGTGA Serial No. 77 of the tissue inhibitor of metalloproteinase > gi | 4507508 | ref | NM-003254.1 | tissue of a human metalloproteinase inhibition Agent (red blood cell synergistic activity, collagenase inhibitor) (TIMPl), mRNA|qPCR advance _ primer matching [221..241] | 9? 逆 reverse _ primer matching [359..340]|9? 〇11 probe matches [251..283]

AGGGGCCTTAGCGTGCCGCATCGCCGAGATCCAGCGCCCAGAGAG ACACCAGAGAACCCACCATGGCCCCCTTTGAGCCCCTGGCTTCTGGCATCC TGTTGTTGCTGTGGCTGATAGCCCCCAGCAGGGCCTGCACCTGTGTCCCAC CCCACCCACAGACGGCCTTCTGCAATTCCGACCTCGTCATCAGGGCCAAG TTCGTGGGGACACCAGAAGTCAACCAGACCACCTTATACCAGCGTTATGA GATCAAGATGACCAAGATGTATAAAGGGTTCCAAGCCTTAGGGGATGCCG CTGACATCCGGTTCGTCTACACCCCCGCCATGGAGAGTGTCTGCGGATACT TCCACAGGTCCCACAACCGCAGCGAGGAGTTTCTCATTGCTGGAAAACTG CAGGATGGACTCTTGCACATCACTACCTGCAGTTTCGTGGCTCCCTGGAAC AGCCTGAGCTTAGCTCAGCGCCGGGGCTTCACCAAGACCTACACTGTTGG CTGTGAGGAATGCACAGTGTTTCCCTGTTTATCCATCCCCTGCAAACTGCA GAGTGGCACTCATTGCTTGTGGACGGACCAGCTCCTCCAAGGCTCTGAAA AGGGCTTCCAGTCCCGTCACCTTGCCTGCCTGCCTCGGGAGCCAGGGCTGT GCACCTGGCAGTCCCTGCGGTCCCAGATAGCCTGAATCCTGCCCGGAGTG GAACTGAAGCCTGCACAGTGTCCACCCTGTTCCCACTCCCATCTTTCTTCC GGACAATGAAATAAAGAGTTACCACCCAGC Serial No. 78 secreted protein acidic and rich in cysteine > gi 48675809 Shu Shu Shu ref NM_003118.2 | human secreted protein 95238.doc -37· 1327645 Qualitative, acidic, rich in cysteine (bone stick Protein) (SPARC), mRNA |9?〇1 Advance_Introduction with [788..810]|9卩〇1 Reverse_Introduction and [915·.898] Matching qPCR Probe and [818..839] match

GTTGCCTGTCTCTAAACCCCTCCACATTCCCGCGGTCCTTCAGACTG

CCCGGAGAGCGCGCTCTGCCTGCCGCCTGCCTGCCTGCCACTGAGGGTTCC

CAGCACCATGAGGGCCTGGATCTTCTTTCTCCTTTGCCTGGCCGGGAGGGC

CTTGGCAGCCCCTCAGCAAGAAGCCCTGCCTGATGAGACAGAGGTGGTGG

AAGAAACTGTGGCAGAGGTGACTGAGGTATCTGTGGGAGCTAATCCTGTC

CAGGTGGAAGTAGGAGAATTTGATGATGGTGCAGAGGAAACCGAAGAGG

AGGTGGTGGCGGAAAATCCCTGCCAGAACCACCACTGCAAACACGGCAA

GGTGTGCGAGCTGGATGAGAACAACACCCCCATGTGCGTGTGCCAGGACC

CCACCAGCTGCCCAGCCCCCATTGGCGAGTTTGAGAAGGTGTGCAGCAAT

GACAACAAGACCTTCGACTCTTCCTGCCACTTCTTTGCCACAAAGTGCACC

CTGGAGGGCACCAAGAAGGGCCACAAGCTCCACCTGGACTACATCGGGCC

TTGCAAATACATCCCCCCTTGCCTGGACTCTGAGCTGACCGAATTCCCCCT

GCGCATGCGGGACTGGCTCAAGAACGTCCTGGTCACCCTGTATGAGAGGG

ATGAGGACAACAACCTTCTGACTGAGAAGCAGAAGCTGCGGGTGAAGAA

GATCCATGAGAATGAGAAGCGCCTGGAGGCAGGAGACCACCCCGTGGAG

CTGCTGGCCCGGGACTTCGAGAAGAACTATAACATGTACATCTTCCCTGTA

CACTGGCAGTTCGGCCAGCTGGACCAGCACCCCATTGACGGGTACCTCTC

CCACACCGAGCTGGCTCCACTGCGTGCTCCCCTCATCCCCATGGAGCATTG

CACCACCCGCTTTTTCGAGACCTGTGACCTGGACAATGACAAGTACATCG

CCCTGGATGAGTGGGCCGGCTGCTTCGGCATCAAGCAGAAGGATATCGAC

AAGGATCTTGTGATCTAAATCCACTCCTTCCACAGTACCGGATTCTCTCTT

TAACCCTCCCCTTCGTGTTTCCCCCAATGTTTAAAATGTTTGGATGGTTTGT

TGTTCTGCCTGGAGACAAGGTGCTAACATAGATTTAAGTGAATACATTAA

CGGTGCTAAAAATGAAAATTCTAACCCAAGACATGACATTCTTAGCTGTA

ACTTAACTATTAAGGCCTTTTCCACACGCATTAATAGTCCCATTTTTCTCTT

GCCATTTGTAGCTTTGCCCATTGTCTTATTGGCACATGGGTGGACACGGAT

CTGCTGGGCTCTGCCTTAAACACACATTGCAGCTTCAACTTTTCTCTTTAGT

GTTCTGTTTGAAACTAATACTTACCGAGTCAGACTTTGTGTTCATTTCATTT

CAGGGTCTTGGCTGCCTGTGGGCTTCCCCAGGTGGCCTGGAGGTGGGCAA

AGGGAAGTAACAGACACACGATGTTGTCAAGGATGGTTTTGGGACTAGAG

GCTCAGTGGTGGGAGAGATCCCTGCAGAACCCACCAACCAGAACGTGGTT

TGCCTGAGGCTGTAACTGAGAGAAAGATTCTGGGGCTGTGTTATGAAAAT

ATAGACATTCTCACATAAGCCCAGTTCATCACCATTTCCTCCTTTACCTTTC

AGTGCAGTTTCTTTTCACATTAGGCTGTTGGTTCAAACTTTTGGGAGCACG

GACTGTCAGTTCTCTGGGAAGTGGTCAGCGCATCCTGCAGGGCTTCTCCTC

CTCTGTCTTTTGGAGAACCAGGGCTCTTCTCAGGGGCTCTAGGGACTGCCA

GGCTGTTTCAGCCAGGAAGGCCAAAATCAAGAGTGAGATGTAGAAAGTTG 95238.doc -38- 1327645

TAAAATAGAAAAAGTGGAGTTGGTGAATCGGTTGTTCTTTCCTCACATTTG

GATGATTGTCATAAGGTTTTTAGCATGTTCCTCCTTTTCTTCACCCTCCCCT

TTTTTCTTCTATTAATCAAGAGAAACTTCAAAGTTAATGGGATGGTCGGAT

CTCACAGGCTGAGAACTCGTTCACCTCCAAGCATTTCATGAAAAAGCTGC

TTCTTATTAATCATACAAACTCTCACCATGATGTGAAGAGTTTCACAAATC

CTTCAAAATAAAAAGTAATGACTTAGAAACTGCCTTCCTGGGTGATTTGC

ATGTGTCTTAGTCTTAGTCACCTTATTATCCTGACACAAAAACACATGAGC

ATACATGTCTACACATGACTACACAAATGCAAACCTTTGCAAACACATTA

TGCTTTTGCACACACACACCTGTACACACACACCGGCATGTTTATACACAG

GGAGTGTATGGTTCCTGTAAGCACTAAGTTAGCTGTTTTCATTTAATGACC

TGTGGTTTAACCCTTTTGATCACTACCACCATTATCAGCACCAGACTGAGC

AGCTATATCCTTTTATTAATCATGGTCATTCATTCATTCATTCATTCACAAA

ATATTTATGATGTATTTACTCTGCACCAGGTCCCATGCCAAGCACTGGGGA

CACAGTTATGGCAAAGTAGACAAAGCATTTGTTCATTTGGAGCTTAGAGT

CCAGGAGGAATACATTAGATAATGACACAATCAAATATAAATTGCAAGAT

GTCACAGGTGTGATGAAGGGAGAGTAGGAGAGACCATGAGTATGTGTAA

CAGGAGGACACAGCATTATTCTAGTGCTGTACTGTTCCGTACGGCAGCCA

CTACCCACATGTAACTTTTTAAGATTTAAATTTAAATTAGTTAACATTCAA

AACGCAGCTCCCCAATCACACTAGCAACATTTCAAGTGCTTGAGAGCCAT

GCATGATTAGTGGTTACCCTATTGAATAGGTCAGAAGTAGAATCTTTTCAT

CATCACAGAAAGTTCTATTGGACAGTGCTCTTCTAGATCATCATAAGACTA

CAGAGCACTTTTCAAAGCTCATGCATGTTCATCATGTTAGTGTCGTATTTT

GAGCTGGGGTTTTGAGACTCCCCTTAGAGATAGAGAAACAGACCCAAGAA

ATGTGCTCAATTGCAATGGGCCACATACCTAGATCTCCAGATGTCATTTCC

CCTCTCTTATTTTAAGTTATGTTAAGATTACTAAAACAATAAAAGCTCCTA

AAAAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA Sequence No. 79

Transforming growth factor, p-induced > gi丨4507466丨ref丨NM_000358.1丨 human transforming growth factor, β-induced, 68 kDa (TGFBI), mRNA| qPCR was determined at the time of the request and [170. 194] Matching

GCTTGCCCGTCGGTCGCTAGCTCGCTCGGTGCGCGTCGTCCCGCTCC

ATGGCGCTCTTCGTGCGGCTGCTGGCTCTCGCCCTGGCTCTGGCCCTGGGC

CCCGCCGCGACCCTGGCGGGTCCCGCCAAGTCGCCCTACCAGCTGGTGCT

GCAGCACAGCAGGCTCCGGGGCCGCCAGCACGGCCCCAACGTGTGTGCTG

TGCAGAAGGTTATTGGCACTAATAGGAAGTACTTCACCAACTGCAAGCAG

TGGTACCAAAGGAAAATCTGTGGCAAATCAACAGTCATCAGCTACGAGTG

CTGTCCTGGATATGAAAAGGTCCCTGGGGAGAAGGGCTGTCCAGCAGCCC

TACCACTCTCAAACCTTTACGAGACCCTGGGAGTCGTTGGATCCACCACCA 95238.doc •39- 1327645

CTCAGCTGTACACGGACCGCACGGAGAAGCTGAGGCCTGAGATGGAGGG

GCCCGGCAGCTTCACCATCTTCGCCCCTAGCAACGAGGCCTGGGCCTCCTT

GCCAGCTGAAGTGCTGGACTCCCTGGTCAGCAATGTCAACATTGAGCTGC

TCAATGCCCTCCGCTACCATATGGTGGGCAGGCGAGTCCTGACTGATGAG

CTGAAACACGGCATGACCCTCACCTCTATGTACCAGAATTCCAACATCCA

GATCCACCACTATCCTAATGGGATTGTAACTGTGAACTGTGCCCGGCTCCT

GAAAGCCGACCACCATGCAACCAACGGGGTGGTGCACCTCATCGATAAGG

TCATCTCCACCATCACCAACAACATCCAGCAGATCATTGAGATCGAGGAC

ACCTTTGAGACCCTTCGGGCTGCTGTGGCTGCATCAGGGCTCAACACGAT

GCTTGAAGGTAACGGCCAGTACACGCTTTTGGCCCCGACCAATGAGGCCT

TCGAGAAGATCCCTAGTGAGACTTTGAACCGTATCCTGGGCGACCCAGAA

GCCCTGAGAGACCTGCTGAACAACCACATCTTGAAGTCAGCTATGTGTGC

TGAAGCCATCGTTGCGGGGCTGTCTGTAGAGACCCTGGAGGGCACGACAC

TGGAGGTGGGCTGCAGCGGGGACATGCTCACTATCAACGGGAAGGCGATC

ATCTCCAATAAAGACATCCTAGCCACCAACGGGGTGATCCACTACATTGA

TGAGCTACTCATCCCAGACTCAGCCAAGACACTATTTGAATTGGCTGCAG

AGTCTGATGTGTCCACAGCCATTGACCTTTTCAGACAAGCCGGCCTCGGCA

ATCATCTCTCTGGAAGTGAGCGGTTGACCCTCCTGGCTCCCCTGAATTCTG

TATTCAAAGATGGAACCCCTCCAATTGATGCCCATACAAGGAATTTGCTTC

GGAACCACATAATTAAAGACCAGCTGGCCTCTAAGTATCTGTACCATGGA

CAGACCCTGGAAACTCTGGGCGGCAAAAAACTGAGAGTTTTTGTTTATCG

TAATAGCCTCTGCATTGAGAACAGCTGCATCGCGGCCCACGACAAGAGGG

GGAGGTACGGGACCCTGTTCACGATGGACCGGGTGCTGACCCCCCCAATG

GGGACTGTCATGGATGTCCTGAAGGGAGACAATCGCTTTAGCATGCTGGT

AGCTGCCATCCAGTCTGCAGGACTGACGGAGACCCTCAACCGGGAAGGAG

TCTACACAGTCTTTGCTCCCACAAATGAAGCCTTCCGAGCCCTGCCACCAA

GAGAACGGAGCAGACTCTTGGGAGATGCCAAGGAACTTGCCAACATCCTG

AAATACCACATTGGTGATGAAATCCTGGTTAGCGGAGGCATCGGGGCCCT

GGTGCGGCTAAAGTCTCTCCAAGGTGACAAGCTGGAAGTCAGCTTGAAAA

ACAATGTGGTGAGTGTCAACAAGGAGCCTGTTGCCGAGCCTGACATCATG

GCCACAAATGGCGTGGTCCATGTCATCACCAATGTTCTGCAGCCTCCAGCC

AACAGACCTCAGGAAAGAGGGGATGAACTTGCAGACTCTGCGCTTGAGAT

CTTCAAACAAGCATCAGCGTTTTCCAGGGCTTCCCAGAGGTCTGTGCGACT

AGCCCCTGTCTATCAAAAGTTATTAGAGAGGATGAAGCATTAGCTTGAAG

CACTACAGGAGGAATGCACCACGGCAGCTCTCCGCCAATTTCTCTCAGAT

TTCCACAGAGACTGTTTGAATGTTTTCAAAACCAAGTATCACACTTTAATG

TACATGGGCCGCACCATAATGAGATGTGAGCCTTGTGCATGTGGGGGAGG

AGGGAGAGAGATGTACTTTTTAAATCATGTTCCCCCTAAACATGGCTGTTA

ACCCACTGCATGCAGAAACTTGGATGTCACTGCCTGACATTCACTTCCAGA

GAGGACCTATCCCAAATGTGGAATTGACTGCCTATGCCAAGTCCCTGGAA

AAGGAGCTTCAGTATTGTGGGGCTCATAAAACATGAATCAAGCAATCCAG

CCTCATGGGAAGTCCTGGCACAGTTTTTGTAAAGCCCTTGCACAGCTGGA

GAAATGGCATCATTATAAGCTATGAGTTGAAATGTTCTGTCAAATGTGTCT 95238.doc -40- 1327645 CACATCTACACGTGGCTTGGAGGCTTTTATGGGGCCCTGTCCAGGTAGAA AAGAAATGGTATGTAGAGCTTAGATTTCCCTATTGTGACAGAGCCATGGT GTGTTTGTAATAATAAAACCAAAGAAACATA Sequence No. 80 contains EGF and the like - phenanthrene extracellular matrix protein 2 >gi | 8393298 | ref | NM—01693 8.1 | Humans containing EGF like phenoline Extracellular matrix protein 2 (EFEMP2), mRNA | qPCR is determined in the context of the requirement and matched with [1248·. 1272].

CAAGCTTGGCACGAGGGCAGGCATTGCCCGAGCCAGCCGAGCCGC

CAGAGCCGCGGGCCGCGCGGGTGTCGCGGGCCCAACCCCAGGATGCTCCC

CTGCGCCTCCTGCCTACCCGGGTCTCTACTGCTCTGGGCGCTGCTACTGTT

GCTCTTGGGATCAGCTTCTCCTCAGGATTCTGAAGAGCCCGACAGCTACAC

GGAATGCACAGATGGCTATGAGTGGGACCCAGACAGCCAGCACTGCCGG

GATGTCAACGAGTGTCTGACCATCCCTGAGGCCTGCAAGGGGGAAATGAA

GTGCATCAACCACTACGGGGGCTACTTGTGCCTGCCCCGCTCCGCTGCCGT

CATCAACGACCTACACGGCGAGGGACCCCCGCCACCAGTGCCTCCCGCTC

AACACCCCAACCCCTGCCCACCAGGCTATGAGCCCGACGATCAGGACAGC

TGTGTGGATGTGGACGAGTGTGCCCAGGCCCTGCACGACTGTCGCCCCAG

CCAGGACTGCCATAACTTGCCTGGCTCCTATCAGTGCACCTGCCCTGATGG

TTACCGCAAGATCGGGCCCGAGTGTGTGGACATAGACGAGTGCCGCTACC

GCTACTGCCAGCACCGCTGCGTGAACCTGCCTGGCTCCTTCCGCTGCCAGT

GCGAGCCGGGCTTCCAGCTGGGGCCTAACAACCGCTCCTGTGTTGATGTG

AACGAGTGTGACATGGGGGCCCCATGCGAGCAGCGCTGCTTCAACTCCTA

TGGGACCTTCCTGTGTCGCTGCCACCAGGGCTATGAGCTGCATCGGGATG

GCTTCTCCTGCAGTGATATTGATGAGTGTAGCTACTCCAGCTACCTCTGTC

AGTACCGCTGCGTCAACGAGCCAGGCCGTTTCTCCTGCCACTGCCCACAG

GGTTACCAGCTGCTGGCCACACGCCTCTGCCAAGACATTGATGAGTGTGA

GTCTGGTGCGCACCAGTGCTCCGAGGCCCAAACCTGTGTCAACTTCCATG

GGGGCTACCGCTGCGTGGACACCAACCGCTGCGTGGAGCCCTACATCCAG

GTCTCTGAGAACCGCTGTCTCTGCCCGGCCTCCAACCCTCTATGTCGAGAG

CAGCCTTCATCCATTGTGCACCGCTACATGACCATCACCTCGGAGCGGAG

AGTACCCGCTGACGTGTTCCAGATCCAGGCGACCTCCGTCTACCCCGGTGC

CTACAATGCCTTTCAGATCCGTGCTGGAAACTCGCAGGGGGACTTTTACAT

TAGGCAAATCAACAACGTCAGCGCCATGCTGGTCCTCGCCCGGCCGGTGA

CGGGCCCCCGGGAGTACGTGCTGGACCTGGAGATGGTCACCATGAATTCC

CTCATGAGCTACCGGGCCAGCTCTGTACTGAGGCTCACCGTCTTTGTAGGG

GCCTACACCTTCTGAGGAGCAGGAGGGAGCCACCCTCCCTGCAGCTACCC

TAGCTGAGGAGCCTGTTGTGAGGGGCAGAATGAGAAAGGCCCAGGGGCC

CCCATTGACAGGAGCTGGGAGCTCTGCACCACGAGCTTCAGTCACCCCGA

GAGGAGAGGAGGTAACGAGGAGGGCGGACTCCAGGCCCCGGCCCAGAGA 95238.doc • 41 · 1327645 TTTGGACTTGGCTGGCTTGCAGGGGTCCTAAGAAACTCCACTCTGGACAG CGCCAGGAGGCCCTGGGTTCCATTCCTAACTCTGCCTCAAACTGTACATTT GGATAAGCCCTAGTAGTTCCCTGGGCCTGTTTTTCTATAAAACGAGGCAAC TGG Lumi Ken SEQ ID NO 81 > gi I 21359858 I ref I ΝΜ_002345 · 2 I Human Lumi Ken (LUM), mRNA I qPCR primer and proceeds _ [61 .. 84] Matching 丨qPCR reverse-introduction matches [182..162] I qPCR probe matches [117·.152]

GTATCACTCAGAATCTGGCAGCCAGTTCCGTCCTGACAGAGTTCAC

AGCATATATTGGTGGATTCTTGTCCATAGTGCATCTGCTTTAAGAATTAAC

GAAAGCAGTGTCAAGACAGTAAGGATTCAAACCATTTGCCAAAAATGAGT

CTAAGTGCATTTACTCTCTTCCTGGCATTGATTGGTGGTACCAGTGGCCAG

TACTATGATTATGATTTTCCCCTATCAATTTATGGGCAATCATCACCAAAC

TGTGCACCAGAATGTAACTGCCCTGAAAGCTACCCAAGTGCCATGTACTG

TGATGAGCTGAAATTGAAAAGTGTACCAATGGTGCCTCCTGGAATCAAGT

ATCTTTACCTTAGGAATAACCAGATTGACCATATTGATGAAAAGGCCTTTG

AGAATGTAACTGATCTGCAGTGGCTCATTCTAGATCACAACCTTCTAGAA

AACTCCAAGATAAAAGGGAGAGTTTTCTCTAAATTGAAACAACTGAAGAA

GCTGCATATAAACCACAACAACCTGACAGAGTCTGTGGGCCCACTTCCCA

AATCTCTGGAGGATCTGCAGCTTACTCATAACAAGATCACAAAGCTGGGC

TCTTTTGAAGGATTGGTAAACCTGACCTTCATCCATCTCCAGCACAATCGG

CTGAAAGAGGATGCTGTTTCAGCTGCTTTTAAAGGTCTTAAATCACTCGAA

TACCTTGACTTGAGCTTCAATCAGATAGCCAGACTGCCTTCTGGTCTCCCT

GTCTCTCTTCTAACTCTCTACTTAGACAACAATAAGATCAGCAACATCCCT

GATGAGTATTTCAAGCGTTTTAATGCATTGCAGTATCTGCGTTTATCTCAC

AACGAACTGGCTGATAGTGGAATACCTGGAAATTCTTTCAATGTGTCATCC

CTGGTTGAGCTGGATCTGTCCTATAACAAGCTTAAAAACATACCAACTGTC

AATGAAAACCTTGAAAACTATTACCTGGAGGTCAATCAACTTGAGAAGTT

TGACATAAAGAGCTTCTGCAAGATCCTGGGGCCATTATCCTACTCCAAGA

TCAAGCATTTGCGTTTGGATGGCAATCGCATCTCAGAAACCAGTCTTCCAC

CGGATATGTATGAATGTCTACGTGTTGCTAACGAAGTCACTCTTAATTAAT

ATCTGTATCCTGGAACAATATTTTATGGTTATGTTTTTCTGTGTGTCAGTTT

TCATAGTATCCATATTTTATTACTGTTTATTACTTCCATGAATTTTAAAATC

TGAGGGAAATGTTTTGTAAACATTTATTTTTTTTAAAGAAAAGATGAAAG

GCAGGCCTATTTCATCACAAGAACACACACATATACACGAATAGACATCA

AACTCAATGCTTTATTTGTAAATTTAGTGTTTTTTTATTTCTACTGTCAAAT

GATGTGCAAAACCTTTTACTGGTTGCATGGAAATCAGCCAAGTTTTATAAT

CCTTAAATCTTAATGTTCCTCAAAGCTTGGATTAAATACATATGGATGTTA

CTCTCTTGCACCAAATTATCTTGATACATTCAAATTTGTCTGGTTAAAAAA 95238.doc -42- 1327645 TAGGTGGTAGATATTGAGGCCAAGAATATTGCAAAATACATGAAGCTTCA TGCACTTAAAGAAGTATTTTTAGAATAAGAATTTGCATACTTACCTAGTGA AACTTTTCTAGAATTATTTTTCACTCTAAGTCATGTATGTTTCTCTTTGATT ATTTGCATGTTATGTTTAATAAGCTACTAGCAAAATAAAACATAGCAAAT GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA Sequence No. 82

>gi I 29893560 I ref I ΝΜ_003498·3 丨 Human Stening (SNN), mRNA

AGCGGGGCCGGACCGGGCGGGCGGAGCCGGGCCCGCGGGGCTGCT

GCGGGGCGATCGGGCCGGGCCGCTGCCGCGCCATGGACTCCCGTGTCCAG

CCTGAGTTCCAGCCTCACTGAGTGGCCACCCCCAAAGTGCTGCCAGCCGA

GGAAGCCCCCAGCACTGACCATGTCTATTATGGACCACAGCCCCACCACG

GGCGTGGTCACAGTCATCGTCATCCTCATTGCCATCGCGGCCCTGGGGGCC

TTGATCCTGGGCTGCTGGTGCTACCTGCGGCTGCAGCGCATCAGCCAGTCA

GAGGACGAGGAGAGCATCGTGGGGGATGGGGAGACCAAGGAACCCTTCC

TGCTGGTGCAGTATTCGGCCAAGGGACCGTGCGTGGAGAGAAAGGCCAA

GCTGATGACTCCCAACGGCCCGGAAGTCCACGGCTGAGCCAGGATGCAAG

GCTCCTGGTCCTGTTTGCAGCCGGCCAAGAGGCGCTGGGAGGGGCAAAAC

CATACGGATGCGCTGCTGTCTGAGAGGAAGGGCTGACACTTGCTGGCATG

GCCTCTGCGGGCTTCGTCATCGCATGCACTGATGCCCGGGGACCTGGCTGT

CCTGGGCTTCCCCTCGGCCTCCAGGTGAGGCTGCCCATTGCAGGCACTGG

GCAGGCCTGACCTTGCTGGGGCTCATGGCCCTGTAGCGCTTTTGTTACTTG

AATGTCTAGCTGAGCCTGTTTTTGATGGAGCTACTACTGTAATGCGTGAAC

TAACAAACCTGTGAACTGTAAATAGGCCCCTGGAAGCACGTGCTTAAGCC

CTTTTGCTGATTTTTAAAAATATCATCTAGCGCACACGGGACTGGTATTCT

GGCTGTACTAATGACAAGCTGAGTCAAGACCCTGGAGGGTCATAGGCTTG

TAAAGGCCCACGCCACACTCGGCAGGGGTCTCTCATGTGTGTCCATCTGC

GTGTATGTCAAGGAAGTGAGATGCCAATTTGGGGTCTTGAGGCTGACCAG

TTGGGGTGCTTGGGTGATCTCTGCTTCATTAGTCATGGGTGGAAGAAAAA

CCACACCCCCCGCACCCCTCCGTTCTTTCTGCATAGACTCACTTGTTAAAT

AGCAGTTCTGTTGAGAGTGGAGTTACTGCAGGGAAGCTACCGGACCTGCC

TGGGAGCCAGTGAAGGGCGAGTCAGGGCACGCGTCCTGGAGGCTGCCAG

CGTCCTTGTAGCAGAGCAGTTTCTTGCCGCTTGGGTCTTCAGCACGCCAAG

CCCCCCACCAACCCTCCACCCCGAGTGAAGGCTTCGCTGAAATTGCTTTGG

TCCTCATAGAGCCTGTGGTGGCTACTTTTGGTCTGAAACCCACTTGGCCCA

GGAAAGAGAAAAGGTTGTATGTTTTGTGTTGGTGTTTCCTATTTTCTGCAC

TGGAGGGGAGGGGACTGTTGAGGTTCTGTCTTTTTTCTTCTTTTCCTCTTCC

CTCTTCACATCACTTGGCTTCCTTTCCTCTCTGATGACCGTCCGCCTATGGG

GTTCTGACTTCACTTTCCTCAGCGGGTCTCCAGTCCCCTGACCCAGCTCTA

AAGGCACTTAGGACCCAGGGAACATTTCTCACGTGCACATTCCCCTAAGA 95238.doc -43- 1327645

GCCACCAGACTGCTTCCTGCCAGCCTGTGCTTGCGGCAGGGAGCCGGGGC AGGGCAGAGGTGAACTTGAAGTTCAGGACTTGACTCTCCCACAGGTGGTG AGCTGGTGGCTCTCTGGTGAGCTAGTGTCTCCACAGCCTGTCTCCAAGGCC TCCCCTATGTACATTTCAGTGAGCTCACTTTGATTTTTAATCCCACCACAA GCACATACTAATTTTATTTATGATTCAAATGTGACTCGTGCCTGCCCATCC CTGTAATAGATGGAAGGTCAGCCCCGGCTTAACCACAGAGCACTGGCCCT TCATGGCTGAGCTCAGAGCTCTGGCCTCCTGCTCAGACTAAAGGCACCTCC TCTGGCCTCACCCAAGCCTCTTCTAAAAACCATGTTGAATGAATCCACGTT CTGGAACCCCGAGGCGGGAGAAGTAGGGAGCTGTTCGTTTAAGCAGCATA CACCTAAATTGGGGGTTTAAACATTAAGTAGGAGCTTGGGGTGGAAGAGG GACAGCCGGCTGGGCCACCTGAGCAGAAGGTGGTAATGAAACACCTCAG CTGGGCTCTTGGGAGACCTTAGGAAGCAGGAGAGGCAACACCTCTGGCTA CTGATGGTGTGGCAAGTTCAGAAGAGGTGGTGGTGGGGTAGGCGTGATGT CAGCAGAAGCCCTGCAGGCTGGGTGGGCAGGACACGTGGTGGGGGCCAC TGAAACCAGGCCTAGGAGGGAGAACAAGTTCCAAAGGTGCCGACTGGAA GAAGGGGGTAAAAGTTTGCTTTGGTGAGTGAGAAAAGGCTGGGGCGTGTG ATCCATCCCCTCACGTTTCAGAACTTCCAGGCTTTCTACCTCGACTCTCAC CACAGCCAGCACATACACCTAGGCTGTTTTTCCTTCCTCCACACCTGAGGG ACGCAGCAACAGCTAGGATCTGCATTTTCAGGTTCCGAGCCTGACCCCTG GAACTGACCAGCGCTCGATTGTCAGCC TTGGCCTGGGGTTTTGACCTTGCC AGTGAAGTTTCGGTTTTGAAGTGATTAAATGTCACTTCCTCATCAGTTTCA CTTCTGGAGGTTTTCTTATCCTACTCCCTGGTGCCAGGGACGTACCTGGGA GTTTGAATCAGGCCCATTTGAGCGTGGCAGCCGTGTTGGGTGAAGGTCCG GGGCTCGGTGAGGCACTGGGGGGGTTTTCGGGAGGAAAATGAAAATGCTT CTAGAATGAGTGAACCACATCATAGCTCTCACTGTTTTTTCAATAGCTACT TTTTTTAGCAGACACCAGAGCCACACTCAAATGGCTAAGTAGGTTATGAC CTCTCTGGATTATTTTTGAATGCCCAACTGTTGCATTCAAGTTTTCTGACTA ATAAGAAATTAAGCATTCATCCTTCGTATCACTGCAGAAGCAACAGTGGG GGCACAGGGAGGGAACTCTTGACACTGAGCCACTAAAATATGGACTAATT TTTTGGACAAATCTTCAAACGGACTGTGCTACTGTATTTGTCTCAAAGCTA CCAAGTTTGTGCAATAAGTGGAAGGGATGTCATCCTTCTTCAATAAATGCT GAATGACATTCAAGCTGATTTTCTAGACCACTGAGAAAATCTTTATTTACA ATAAATTTCAATAAAATTTGCATAAATATATTCCCAAAAAAAAAAAAAAA AAAAAAGAAAAAAAAAAAAA Serial No. 83 secreted phosphoprotein 1 > gi 38146097 Shu Shu Shu ref NM_000582.2 Shu human secreted phosphoprotein 1 (osteoblast protein, bone sialoprotein Protein I, early T-lymphocyte activation 1) (SPP1), mRNA | qPCR is determined at the time of the request and is matched with [253..277] 95238.doc -44· 1327645

CTCCCTGTGTTGGTGGAGGATGTCTGCAGCAGCATTTAAATTCTGG GAGGGCTTGGTTGTCAGCAGCAGCAGGAGGAGGCAGAGCACAGCATCGT CGGGACCAGACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCG ACCAAGGAAAACTCACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCC TAGGCATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAAGTTCTG AGGAAAAGCAGCTTTACAACAAATACCCAGATGCTGTGGCCACATGGCTA AACCCTGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAGACCCTTCC AAGTAAGTCCAACGAAAGCCATGACCACATGGATGATATGGATGATGAA GATGATGATGACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTC TGATGATGTAGATGACACTGATGATTCTCACCAGTCTGATGAGTCTCACCA TTCTGATGAATCTGATGAACTGGTCACTGATTTTCCCACGGACCTGCCAGC AACCGAAGTTTTCACTCCAGTTGTCCCCACAGTAGACACATATGATGGCC GAGGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAGAAGTTTCGC AGACCTGACATCCAGTACCCTGATGCTACAGACGAGGACATCACCTCACA CATGGAAAGCGAGGAGTTGAATGGTGCATACAAGGCCATCCCCGTTGCCC AGGACCTGAACGCGCCTTCTGATTGGGACAGCCGTGGGAAGGACAGTTAT GAAACGAGTCAGCTGGATGACCAGAGTGCTGAAACCCACAGCCACAAGC AGTCCAGATTATATAAGCGGAAAGCCAATGATGAGAGCAATGAGCATTCC GATGTGATTGATAGTCAGGAACTTTCCAAAGTCAGCCGTGAATTCCACAG CCATGAATTTCACAGCCATGAAGATATGCTGGTT GTAGACCCCAAAAGTA AGGAAGAAGATAAACACCTGAAATTTCGTATTTCTCATGAATTAGATAGT GCATCTTCTGAGGTCAATTAAAAGGAGAAAAAATACAATTTCTCACTTTG CATTTAGTCAAAAGAAAAAATGCTTTATAGCAAAATGAAAGAGAACATGA AATGCTTCTTTCTCAGTTTATTGGTTGAATGTGTATCTATTTGAGTCTGGAA ATAACTAATGTGTTTGATAATTAGTTTAGTTTGTGGCTTCATGGAAACTCC CTGTAAACTAAAAGCTTCAGGGTTATGTCTATGTTCATTCTATAGAAGAAA TGCAAACTATCACTGTATTTTAATATTTGTTATTCTCTCATGAATAGAAATT TATGTAGAAGCAAACAAAATACTTTTACCCACTTAAAAAGAGAATATAAC ATTTTATGTCACTATAATCTTTTGTTTTTTAAGTTAGTGTATATTTTGTTGT GATTATCTTTTTGTGGTGTGAATAAATCTTTTATCTTGAATGTAATAAGAA TTTGGTGGTGTCAATTGCTTATTTGTTTTCCCACGGTTGTCCAGCAATTAAT AAAACATAACCTTTTTTACTGCCTAAAAAAAAAAAAAAAAAAAAAAAAA Serial No. 84 AAAAAAAAA chondroitin sulfate proteoglycan 2 > gi | 21361115 | ref | NM_0043 85.2 | human chondroitin sulfate proteoglycan 2 (Fu Xiken) (CSPG2), mRNA丨qPCR Advance—Introduction matches [10087..10106]丨9?01 Reverse_Introduction matches [10185..10163]丨qPCR probe matches [10139..10161] 95238.doc • 45· 1327645

GCTGCCCCGAGCCTTTCTGGGGAAGAACTCCAGGCGTGCGGACGCA

ACAGCCGAGAACATTAGGTGTTGTGGACAGGAGCTGGGACCAAGATCTTC

GGCCAGCCCCGCATCCTCCCGCATCTTCCAGCACCGTCCCGCACCCTCCGC

ATCCTTCCCCGGGCCACCACGCTTCCTATGTGACCCGCCTGGGCAACGCCG

AACCCAGTCGCGCAGCGCTGCAGTGAATTTTCCCCCCAAACTGCAATAAG

CCGCCTTCCAAGGCCAAGATGTTCATAAATATAAAGAGCATCTTATGGAT

GTGTTCAACCTTAATAGTAACCCATGCGCTACATAAAGTCAAAGTGGGAA

AAAGCCCACCGGTGAGGGGCTCCCTCTCTGGAAAAGTCAGCCTACCTTGT

CATTTTTCAACGATGCCTACTTTGCCACCCAGTTACAACACCAGTGAATTT

CTCCGCATCAAATGGTCTAAGATTGAAGTGGACAAAAATGGAAAAGATTT

GAAAGAGACTACTGTCCTTGTGGCCCAAAATGGAAATATCAAGATTGGTC

AGGACTACAAAGGGAGAGTGTCTGTGCCCACACATCCCGAGGCTGTGGGC

GATGCCTCCCTCACTGTGGTCAAGCTGCTGGCAAGTGATGCGGGTCTTTAC

CGCTGTGACGTCATGTACGGGATTGAAGACACACAAGACACGGTGTCACT

GACTGTGGATGGGGTTGTGTTTCACTACAGGGCGGCAACCAGCAGGTACA

CACTGAATTTTGAGGCTGCTCAGAAGGCTTGTTTGGACGTTGGGGCAGTC

ATAGCAACTCCAGAGCAGCTCTTTGCTGCCTATGAAGATGGATTTGAGCA

GTGTGACGCAGGCTGGCTGGCTGATCAGACTGTCAGATATCCCATCCGGG

CTCCCAGAGTAGGCTGTTATGGAGATAAGATGGGAAAGGCAGGAGTCAG

GACTTATGGATTCCGTTCTCCCCAGGAAACTTACGATGTGTATTGTTATGT

GGATCATCTGGATGGTGATGTGTTCCACCTCACTGTCCCCAGTAAATTCAC

CTTCGAGGAGGCTGCAAAAGAGTGTGAAAACCAGGATGCCAGGCTGGCA

ACAGTGGGGGAACTCCAGGCGGCATGGAGGAACGGCTTTGACCAGTGCG

ATTACGGGTGGCTGTCGGATGCCAGCGTGCGCCACCCTGTGACTGTGGCC

AGGGCCCAGTGTGGAGGTGGTCTACTTGGGGTGAGAACC’CTGTATCGTTT

TGAGAACCAGACAGGCTTCCCTCCCCCTGATAGCAGATTTGATGCCTACTG

CTTTAAACCTAAAGAGGCTACAACCATCGATTTGAGTATCCTCGCAGAAA

CTGCATCACCCAGTTTATCCAAAGAACCACAAATGGTTTCTGATAGAACT

ACACCAATCATCCCTTTAGTTGATGAATTACCTGTCATTCCAACAGAGTTC

CCTCCCGTGGGAAATATTGTCAGTTTTGAACAGAAAGCCACAGTCCAACC

TCAGGCTATCACAGATAGTTTAGCCACCAAATTACCCACACCTACTGGCA

GTACCAAGAAGCCCTGGGATATGGATGACTACTCACCTTCTGCTTCAGGA

CCTCTTGGAAAGCTAGACATATCAGAAATTAAGGAAGAAGTGCTCCAGAG

TACAACTGGCGTCTCTCATTATGCTACGGATTCATGGGATGGTGTCGTGGA

AGATAAACAAACACAAGAATCGGTTACACAGATTGAACAAATAGAAGTG

GGTCCTTTGGTAACATCTATGGAAATCTTAAAGCACATTCCTTCCAAGGAA

TTCCCTGTAACTGAAACACCATTGGTAACTGCAAGAATGATCCTGGAATC

CAAAACTGAAAAGAAAATGGTAAGCACTGTTTCTGAATTGGTAACCACAG

GTCACTATGGATTCACCTTGGGAGAAGAGGATGATGAAGACAGAACACTT

ACAGTTGGATCTGATGAGAGCACCTTGATCTTTGACCAAATTCCTGAAGTC

ATTACGGTGTCAAAGACTTCAGAAGACACCATCCACACTCATTTAGAAGA

CTTGGAGTCAGTCTCAGCATCCACAACTGTTTCCCCTTTAATTATGCCTGA

TAATAATGGATCATCCATGGATGACTGGGAAGAGAGACAAACTAGTGGTA 95238.doc -46- 1327645

GGATAACGGAAGAGTTTCTTGGCAAATATCTGTCTACTACACCTTTTCCAT

CACAGCATCGTACAGAAATAGAATTGTTTCCTTATTCTGGTGATAAAATAT

TAGTAGAGGGAATTTCCACAGTTATTTATCCTTCTCTACAAACAGAAATGA

CACATAGAAGAGAAAGAACAGAAACACTAATACCAGAGATGAGAACAGA

TACTTATACAGATGAAATACAAGAAGAGATCACTAAAAGTCCATTTATGG

GAAAAACAGAAGAAGAAGTCTTCTCTGGGATGAAACTCTCTACATCTCTC

TCAGAGCCAATTCATGTTACAGAGTCTTCTGTGGAAATGACCAAGTCTTTT

GATTTCCCAACATTGATAACAAAGTTAAGTGCAGAGCCAACAGAAGTAAG

AGATATGGAGGAAGACTTTACAGCAACTCCAGGTACTACAAAATATGATG

AAAATATTACAACAGTGCTTTTGGCCCATGGTACTTTAAGTGTTGAAGCAG

CCACTGTATCAAAATGGTCATGGGATGAAGATAATACAACATCCAAGCCT

TTAGAGTCTACAGAACCTTCAGCCTCTTCAAAATTGCCCCCTGCCTTACTC

ACAACTGTGGGGATGAATGGAAAGGATAAAGACATCCCAAGTTTCACTGA

AGATGGAGCAGATGAATTTACTCTTATTCCAGATAGTACTCAAAAGCAGT

TAGAGGAGGTTACTGATGAAGACATAGCAGCCCATGGAAAATTCACAATT

AGATTTCAGCCAACTACATCAACTGGTATTGCAGAAAAGTCAACTTTGAG

AGATTCTACAACTGAAGAAAAAGTTCCACCTATCACAAGCACTGAAGGCC

AAGTTTATGCAACCATGGAAGGAAGTGCTTTGGGTGAAGTAGAAGATGTG

GACCTCTCTAAGCCAGTATCTACTGTTCCCCAATTTGCACACACTTCAGAG

GTGGAAGGATTAGCATTTGTTAGTTATAGTAGCACCCAAGAGCCTACTAC

TTATGTAGACTCTTCCCATACCATTCCTCTTTCTGTAATTCCCAAGACAGA

CTGGGGAGTGTTAGTACCTTCTGTTCCATCAGAAGATGAAGTTCTAGGTGA

ACCCTCTCAAGACATACTTGTCATTGAT.CAGACTCGCCTTGAAGCGACTAT

TTCTCCAGAAACTATGAGAACAACAAAAATCACAGAGGGAACAACTCAG

GAAGAATTCCCTTGGAAAGAACAGACTGCAGAGAAACCAGTTCCTGCTCT

CAGTTCTACAGCTTGGACTCCCAAGGAGGCAGTAACACCACTGGATGAAC

AAGAGGGCGATGGATCAGCATATACAGTCTCTGAAGATGAATTGTTGACA

GGTTCTGAGAGGGTCCCAGTTTTAGAAACAACTCCAGTTGGAAAAATTGA

TCACAGTGTGTCTTATCCACCAGGTGCTGTAACTGAGCACAAAGTGAAAA

CAGATGAAGTGGTAACACTAACACCACGCATTGGGCCAAAAGTATCTTTA

AGTCCAGGGCCTGAACAAAAATATGAAACAGAAGGTAGTAGTACAACAG

GATTTACATCATCTTTGAGTCCTTTTAGTACCCACATTACCCAGCTTATGG

AAGAAACCACTACTGAGAAAACATCCCTAGAGGATATTGATTTAGGCTCA

GGATTATTTGAAAAGCCCAAAGCCACAGAACTCATAGAATTTTCAACAAT

CAAAGTCACAGTTCCAAGTGATATTACCACTGCCTTCAGTTCAGTAGACA

GACTTCACACAACTTCAGCATTCAAGCCATCTTCCGCGATCACTAAGAAA

CCACCTCTCATCGACAGGGAACCTGGTGAAGAAACAACCAGTGACATGGT

AATCATTGGAGAATCAACATCTCATGTTCCTCCCACTACCCTTGAAGATAT

TGTAGCCAAGGAAACAGAAACCGATATTGATAGAGAGTATTTCACGACTT

CAAGTCCTCCTGCTACACAGCCAACAAGACCACCCACTGTGGAAGACAAA

GAGGCCTTTGGACCTCAGGCGCTTTCTACGCCACAGCCCCCAGCAAGCAC

AAAATTTCACCCTGACATTAATGTTTATATTATTGAGGTCAGAGAAAATAA

GACAGGTCGAATGAGTGATTTGAGTGTAATTGGTCATCCAATAGATTCAG 95238.doc -47- 1327645

AATCTAAAGAAGATGAACCTTGTAGTGAAGAAACAGATCCAGTGCATGAT

CTAATGGCTGAAATTTTACCTGAATTCCCTGACATAATTGAAATAGACCTA

TACCACAGTGAAGAAAATGAAGAAGAAGAAGAAGAGTGTGCAAATGCTA

CTGATGTGACAACCACCCCATCTGTGCAGTACATAAATGGGAAGCATCTC

GTTACCACTGTGCCCAAGGACCCAGAAGCTGCAGAAGCTAGGCGTGGCCA

GTTTGAAAGTGTTGCACCTTCTCAGAATTTCTCGGACAGCTCTGAAAGTGA

TACTCATCCATTTGTAATAGCCAAAACGGAATTGTCTACTGCTGTGCAACC

TAATGAATCTACAGAAACAACTGAGTCTCTTGAAGTTACATGGAAGCCTG

AGACTTACCCTGAAACATCAGAACATTTTTCAGGTGGTGAGCCTGATGTTT

TCCCCACAGTCCCATTCCATGAGGAATTTGAAAGTGGAACAGCCAAAAAA

GGGGCAGAATCAGTCACAGAGAGAGATACTGAAGTTGGTCATCAGGCAC

ATGAACATACTGAACCTGTATCTCTGTTTCCTGAAGAGTCTTCAGGAGAGA

TTGCCATTGACCAAGAATCTCAGAAAATAGCCTTTGCAAGGGCTACAGAA

GTAACATTTGGTGAAGAGGTAGAAAAAAGTACTTCTGTCACATACACTCC

CACTATAGTTCCAAGTTCTGCATCAGCATATGTTTCAGAGGAAGAAGCAG

TTACCCTAATAGGAAATCCTTGGCCAGATGACCTGTTGTCTACCAAAGAA

AGCTGGGTAGAAGCAACTCCTAGACAAGTTGTAGAGCTCTCAGGGAGTTC

TTCGATTCCAATTACAGAAGGCTCTGGAGAAGCAGAAGAAGATGAAGATA

CAATGTTCACCATGGTAACTGATTTATCACAGAGAAATACTACTGATACA

CTCATTACTTTAGACACTAGCAGGATAATCACAGAAAGCTTTTTTGAGGTT

CCTGCAACCACCATTTATCCAGTTTCTGAACAACCTTCTGCAAAAGTGGTG

CCTACCAAGTTTGTAAGTGAAACAGACACTTCTGAGTGGATTTCCAGTACC

ACTGTTGAGGAAAAGAAAAGGAAGGAGGAGGAGGGAACTACAGGTACGG

CTTCTACATTTGAGGTATATTCATCTACACAGAGATCGGATCAATTAATTT

TACCCTTTGAATTAGAAAGTCCAAATGTAGCTACATCTAGTGATTCAGGTA

CCAGGAAAAGTTTTATGTCCTTGACAACACCAACACAGTCTGAAAGGGAA

ATGACAGATTCTACTCCTGTCTTTACAGAAACAAATACATTAGAAAATTTG

GGGGCACAGACCACTGAGCACAGCAGTATCCATCAACCTGGGGTTCAGGA

AGGGCTGACCACTCTCCCACGTAGTCCTGCCTCTGTCTTTATQ^AGCAGGG

CTCTGGAGAAGCTGCTGCCGACCCAGAAACCACCACTGTTTCTTCATTTTC

ATTAAACGTAGAGTATGCAATTCAAGCCGAAAAGGAAGTAGCTGGCACTT

TGTCTCCGCATGTGGAAACTACATTCTCCACTGAGCCAACAGGACTGGTTT

TGAGTACAGTAATGGACAGAGTAGTTGCTGAAAATATAACCCAAACATCC

AGGGAAATAGTGATTTCAGAGCGATTAGGAGAACCAAATTATGGGGCAG

AAATAAGGGGCTTTTCCACAGGTTTTCCTTTGGAGGAAGATTTCAGTGGTG

ACTTTAGAGAATACTCAACAGTGTCTCATCCCATAGCAAAAGAAGAAACG

GTAATGATGGAAGGCTCTGGAGATGCAGCATTTAGGGACACCCAGACTTC

ACCATCTACAGTACCTACTTCAGTTCACATCAGTCACATATCTGACTCAGA

AGGACCCAGTAGCACCATGGTCAGCACTTCAGCCTTCCCCTGGGAAGAGT

TTACATCCTCAGCTGAGGGCTCAGGTGAGCAACTGGTCACAGTCAGCAGC

TCTGTTGTTCCAGTGCTTCCCAGTGCTGTGCAAAAGTTTTCTGGTACAGCT

TCCTCCATTATCGACGAAGGATTGGGAGAAGTGGGTACTGTCAATGAAAT

TGATAGAAGATCCACCATTTTACCAACAGCAGAAGTGGAAGGTACGAAAG 95238.doc -48- 1327645

CTCCAGTAGAGAAGGAGGAAGTAAAGGTCAGTGGCACAGTTTCAACAAA

CTTTCCCCAAACTATAGAGCCAGCCAAATTATGGTCTAGGCAAGAAGTCA

ACCCTGTAAGACAAGAAATTGAAAGTGAAACAACATCAGAGGAACAAAT

TCAAGAAGAAAAGTCATTTGAATCCCCTCAAAACTCTCCTGCAACAGAAC

AAACAATCTTTGATTCACAGACATTTACTGAAACTGAACTCAAAACCACA

GATTATTCTGTACTAACAACAAAGAAAACTTACAGTGATGATAAAGAAAT

GAAGGAGGAAGACACTTCTTTAGTTAACATGTCTACTCCAGATCCAGATG

CAAATGGCTTGGAATCTTACACAACTCTCCCTGAAGCTACTGAAAAGTCA

CATTTTTTCTTAGCTACTGCATTAGTAACTGAATCTATACCAGCTGAACAT

GTAGTCACAGATTCACCAATCAAAAAGGAAGAAAGTACAAAACATTTTCC

GAAAGGCATGAGACCAACAATTCAAGAGTCAGATACTGAGCTCTTATTCT

CTGGACTGGGATCAGGAGAAGAAGTTTTACCTACTCTACCAACAGAGTCA

GTGAATTTTACTGAAGTGGAACAAATCAATAACACATTATATCCCCACAC

TTCTCAAGTGGAAAGTACCTCAAGTGACAAAATTGAAGACTTTAACAGAA

TGGAAAATGTGGCAAAAGAAGTTGGACCACTCGTATCTCAAACAGACATC

TTTGAAGGTAGTGGGTCAGTAACCAGCACAACATTAATAGAAATTTTAAG

TGACACTGGAGCAGAAGGACCCACGGTGGCACCTCTCCCTTTCTCCACGG

ACATCGGACATCCTCAAAATCAGACTGTCAGGTGGGCAGAAGAAATCCAG

ACTAGTAGACCACAAACCATAACTGAACAAGACTCTAACAAGAATTCTTC

AACAGCAGAAATTAACGAAACAACAACCTCATCTACTGATTTTCTGGCTA

GAGCTTATGGTTTTGAAATGGCCAAAGAATTTGTTACATCAGCACCAAAA

CCATCTGACTTGTATTATGAACCTTCTGGAGAAGGATCTGGAGAAGTGGA

TATTGTTGATTCATTTCACACTTCTGCAACTACTCAGGCAACCAGACAAGA

AAGCAGCACCACATTTGTTTCTGATGGGTCCCTGGAAAAACATCCTGAGG

TGCCAAGCGCTAAAGCTGTTACTGCTGATGGATTCCCAACAGTTTCAGTGA

TGCTGCCTCTTCATTCAGAGCAGAACAAAAGCTCCCCTGATCCAACTAGC

ACACTGTCAAATACAGTGTCATATGAGAGGTCCACAGACGGTAGTTTCCA

AGACCGTTTCAGGGAATTCGAGGATTCCACCTTAAAACCTAACAGAAAAA

AACCCACTGAAAATATTATCATAGACCTGGACAAAGAGGACAAGGATTTA

ATATTGACAATTACAGAGAGTACCATCCTTGAAATTCTACCTGAGCTGAC

ATCGGATAAAAATACTATCATAGATATTGATCATACTAAACCTGTGTATG

AAGACATTCTTGGAATGCAAACAGATATAGATACAGAGGTACCATCAGAA

CCACATGACAGTAATGATGAAAGTAATGATGACAGCACTCAAGTTCAAGA

GATCTATGAGGCAGCTGTCAACCTTTCTTTAACTGAGGAAACATTTGAGG

GCTCTGCTGATGTTCTGGCTAGCTACACTCAGGCAACACATGATGAATCA

ATGACTTATGAAGATAGAAGCCAACTAGATCACATGGGCTTTCACTTCAC

AACTGGGATCCCTGCTCCTAGCACAGAAACAGAATTAGACGTTTTACTTCC

CACGGCAACATCCCTGCCAATTCCTCGTAAGTCTGCCACAGTTATTCCAGA

GATTGAAGGAATAAAAGCTGAAGCAAAAGCCCTGGATGACATGTTTGAAT

CAAGCACTTTGTCTGATGGTCAAGCTATTGCAGACCAAAGTGAAATAATA

CCAACATTGGGCCAATTTGAAAGGACTCAGGAGGAGTATGAAGACAAAA

AACATGCTGGTCCTTCTTTTCAGCCAGAATTCTCTTCAGGAGCTGAGGAGG

CATTAGTAGACCATACTCCCTATCTAAGTATTGCTACTACCCACCTTATGG 95238.doc -49- 1327645

ATCAGAGTGTAACAGAGGTGCCTGATGTGATGGAAGGATCCAATCCCCCA

TATTACACTGATACAACATTAGCAGTTTCAACATTTGCGAAGTTGTCTTCT

CAGACACCATCATCTCCCCTCACTATCTACTCAGGCAGTGAAGCCTCTGGA

CACACAGAGATCCCCCAGCCCAGTGCTCTGCCAGGAATAGACGTCGGCTC

ATCTGTAATGTCCCCACAGGATTCTTTTAAGGAAATTCATGTAAATATTGA

AGCAACTTTCAAACCATCAAGTGAGGAATACCTTCACATAACTGAGCCTC

CCTCTTTATCTCCTGACACAAAATTAGAACCTTCAGAAGATGATGGTAAAC

CTGAGTTATTAGAAGAAATGGAAGCTTCTCCCACAGAACTTATTGCTGTG

GAAGGAACTGAGATTCTCCAAGATTTCCAAAACAAAACCGATGGTCAAGT

TTCTGGAGAAGCAATCAAGATGTTTCCCACCATTAAAACACCTGAGGCTG

GAACTGTTATTACAACTGCCGATGAAATTGAATTAGAAGGTGCTACACAG

TGGCCACACTCTACTTCTGCTTCTGCCACCTATGGGGTCGAGGCAGGTGTG

GTGCCTTGGCTAAGTCCACAGACTTCTGAGAGGCCCACGCTTTCTTCTTCT

CCAGAAATAAACCCTGAAACTCAAGCAGCTTTAATCAGAGGGCAGGATTC

CACGATAGCAGCATCAGAACAGCAAGTGGCAGCGAGAATTCTTGATTCCA

ATGATCAGGCAACAGTAAACCCTGTGGAATTTAATACTGAGGTTGCAACA

CCACCATTTTCCCTTCTGGAGACTTCTAATGAAACAGATTTCCTGATTGGC

ATTAATGAAGAGTCAGTGGAAGGCACGGCAATCTATTTACCAGGACCTGA

TCGCTGCAAAATGAACCCGTGCCTTAACGGAGGCACCTGTTATCCTACTG

AAACTTCCTACGTATGCACCTGTGTGCCAGGATACAGCGGAGACCAGTGT

GAACTTGATTTTGATGAATGTCACTCTAATCCCTGTCGTAATGGAGCCACT

TGTGTTGATGGTTTTAACACATTCAGGTGCCTCTGCCTTCCAAGTTATGTT

GGTGCACTTTGTGAGCAAGATACCGAGACATGTGACTATGGCTGGCACAA

ATTCCAAGGGCAGTGCTACAAATACTTTGCCCATCGACGCACATGGGATG

CAGCTGAACGGGAATGCCGTCTGCAGGGTGCCCATCTCACAAGCATCCTG

TCTCACGAAGAACAAATGTTTGTTAATCGTGTGGGCCATGATTATCAGTGG

ATAGGCCTCAATGACAAGATGTTTGAGCATGACTTCCGTTGGACTGATGG

CAGCACACTGCAATACGAGAATTGGAGACCCAACCAGCCAGACAGCTTCT

TTTCTGCTGGAGAAGACTGTGTTGTAATCATTTGGCATGAGAATGGCCAGT

GGAATGATGTTCCCTGCAATTACCATCTCACCTATACGTGCAAGAAAGGA

ACAGTTGCTTGCGGCCAGCCCCCTGTTGTAGAAAATGCCAAGACCTTTGG

AAAGATGAAACCTCGTTATGAAATCAACTCCCTGATTAGATACCACTGCA

AAGATGGTTTCATTCAACGTCACCTTCCAACTATCCGGTGCTTAGGAAATG

GAAGATGGGCTATACCTAAAATTACCTGCATGAACCCATCTGCATACCAA

AGGACTTATTCTATGAAATACTTTAAAAATTCCTCATCAGCAAAGGACAA

TTCAATAAATACATCCAAACATGATCATCGTTGGAGCCGGAGGTGGCAGG

AGTCGAGGCGCTGATCCCTAAAATGGCGAACATGTGTTTTCATCATTTCAG

CCAAAGTCCTAACTTCCTGTGCCTTTCCTATCACCTCGAGAAGTAATTATC

AGTTGGTTTGGATTTTTGGACCACCGTTCAGTCATTTTGGGTTGCCGTGCT

CCCAAAACATTTTAAATGAAAGTATTGGCATTCAAAAAGACAGCAGACAA

AATGAAAGAAAATGAGAGCAGAAAGTAAGCATTTCCAGCCTATCTAATTT

CTTTAGTTTTCTATTTGCCTCCAGTGCAGTCCATTTCCTAATGTATACCAGC

CTACTGTACTATTTAAAATGCTCAATTTCAGCACCGATGGCCATGTAAATA 95238.doc -50- 1327645 AGATGATTTAATGTTGATTTTAATCCTGTATATAAAATAAAAAGTCACAAT GAGTTTGGGCATATTTAATGATGATTATGGAGCCTTAGAGGTCTTTAATCA TTGGTTCGGCTGCTTTTATGTAGTTTAGGCTGGAAATGGTTTCACTTGCTCT TTGACTGTCAGCAAGACTGAAGATGGCTTTTCCTGGACAGCTAGAAAACA CAAAATCTTGTAGGTCATTGCACCTATCTCAGCCATAGGTGCAGTTTGCTT CTACATGATGCTAAAGGCTGCGAATGGGATCCTGATGGAACTAAGGACTC CAATGTCGAACTCTTCTTTGCTGCATTCCTTTTTCTTCACTTACAAGAAAGG CCTGAATGGAGGACTTTTCTGTAACCAGG Serial No. 85 N- acyl sphingosine Dukes amine hydrolase 1 > gi | 3 0089929 | ref | NM_0043 15.2 Shu human N- acyl sheath Amine decylamine hydrolase (acidic neural guanamine) 1 (ASAH1), transcript variant 2, 11111\8|9?011 advance _ primer matched with [1212..1228]|9?〇1 reverse _ primer and [1290..1266] Matching | qPCR probe matches [1233·.1260]

GGACTTTGAAATCCAACCCGGTCACCTACCCGCGCGACTGTGTCCA

CGGATGGCACGAAAGCCAAGCGAGTCCCCCTGCCGAGCTACTCGCGTCCG

CCTCCTCCCAAGCTGAGCTCTGCTCCGCCCACCTGAGTCCTTCGCCAGTTA

GGAGGAAACACAGCCGCTTAATGAACTGCTGCATCGGGCTGGGAGAGAA

AGCTCGCGGGTCCCACCGGGCCTCCTACCCAAGTCTCAGCGCGCTTTTCAC

CGAGGCCTCAATTCTGGGATTTGGCAGCTTTGCTGTGAAAGCCCAATGGA

CAGAGGACTGCAGAAAATCAACCTATCCTCCTTCAGGACCAACGTACAGA

GGTGCAGTTCCATGGTACACCATAAATCTTGACTTACCACCCTACAAAAG

ATGGCATGAATTGATGCTTGACAAGGCACCAATGCTAAAGGTTATAGTGA

ATTCTCTGAAGAATATGATAAATACATTCGTGCCAAGTGGAAAAGTTATG

CAGGTGGTGGATGAAAAATTGCCTGGCCTACTTGGCAACTTTCCTGGCCCT

TTTGAAGAGGAAATGAAGGGTATTGCCGCTGTTACTGATATACCTTTAGG

AGAGATTATTTCATTCAATATTTTTTATGAATTATTTACCATTTGTACTTCA

ATAGTAGCAGAAGACAAAAAAGGTCATCTAATACATGGGAGAAACATGG

ATTTTGGAGTATTTCTTGGGTGGAACATAAATAATGATACCTGGGTCATAA

CTGAGCAACTAAAACCTTTAACAGTGAATTTGGATTTCCAAAGAAACAAC

AAAACTGTCTTCAAGGCTTCAAGCTTTGCTGGCTATGTGGGCATGTTAACA

GGATTCAAACCAGGACTGTTCAGTCTTACACTGAATGAACGTTTCAGTATA

AATGGTGGTTATCTGGGTATTCTAGAATGGATTCTGGGAAAGAAAGATGC

CATGTGGATAGGGTTCCTCACTAGAACAGTTCTGGAAAATAGCACAAGTT

ATGAAGAAGCCAAGAATTTATTGACCAAGACCAAGATATTGGCCCCAGCC

TACTTTATCCTGGGAGGCAACCAGTCTGGGGAAGGTTGTGTGATTACACG

AGACAGAAAGGAATCATTGGATGTATATGAACTCGATGCTAAGCAGGGTA

GATGGTATGTGGTACAAACAAATTATGACCGTTGGAAACATCCCTTCTTCC

TTGATGATCGCAGAACGCCTGCAAAGATGTGTCTGAACCGCACCAGCCAA 95238.doc •51- 1327645

GAGAATATCTCATTTGAAACCATGTATGATGTCCTGTCAACAAAACCTGTC CTCAACAAGCTGACCGTATACACAACCTTGATAGATGTTACCAAAGGTCA ATTCGAAACTTACCTGCGGGACTGCCCTGACCCTTGTATAGGTTGGTGAGC ACACGTCTGGCCTACAGAATGCGGCCTCTGAGACATGAAGACACCATCTC CATGTGACCGAACACTGCAGCTGTCTGACCTTCCAAAGACTAAGACTCGC GGCAGGTTCTCTTTGAGTCAAAAGCTTGTCTTCGTCCATCTGTTGACAAAT GACAGACCTTTTTTTTTCCCCCATCAGTTGATTTTTCTTATTTACAGATAAC TTCTTTAGGGGAAGTAAAACAGTCATCTAGAATTCACTGAGTTTTGTTTCA CTTTGACATTTGGGGATCTGGTGGGCAGTCGAACCATGGTGAACTCCACCT CCGTGGAATAAATGGAGATTCAGCGTGGGTGTTGAATCCAGCACGTCTGT GTGAGTAACGGGACAGTAAACACTCCACATTCTTCAGTTTTTCACTTCTAC CTACATATTTGTATGTTTTTCTGTATAACAGCCTTTTCCTTCTGGTTCTAAC TGCTGTTAAAATTAATATATCATTATCTTTGCTGTTATTGACAGCGATATA ATTTTATTACATATGATTAGAGGGATGAGACAGACATTCACCTGTATATTT CTTTTAATGGGCACAAAATGGGCCCTTGCCTCTAAATAGCACTTTTTGGGG TTCAAGAAGTAATCAGTATGCAAAGCAATCTTTTATACAATAATTGAAGT GTTCCCTTTTTCATAATTACTGTACTTCCCAGTAACCCTAAGGAAGTTGCT AACTTAAAAAACTGCATCCCACGTTCTGTTAATTTAGTAAATAAACAAGTC AAAGACTTGTGGAAAATAGGAAGTGAACCCATATTTTAAATTCTCATAAG TAGCATTCATGTAATA AACAGGTTTTTAGTTTGTTCTTCAGATTGATAGGG AGTTTTAAAGAAATTTTAGTAGTTACTAAAATTATGTTACTGTATTTTTCA GAAATCAAACTGCTTATGAAAAGTACTAATAGAACTTGTTAACCTTTCTAA CCTTCACGATTAACTGTGAAATGTACGTCATTTGTGCAAGACCGTTTGTCC ACTTCATTTTGTATAATCACAGTTGTGTTCCTGACACTCAATAAACAGTCA TTGGAAAGAGTGCCAGTCAGCAGTCATGCA Serial No. 86 N- acyl sphingosine Amides hydrolase 1 transcript variant 1 > gi 30089927 Shu Shu Shu ref NM_177924.1 Shu human sphingosine N- acyl hydrolase Amides (acidic neural prolamine) 1 (ASAH1), transcript variant 2, 111 «^ gossip 9? €11 advancement - introduction with [1050..1066] | 4? €11 reverse primer and [1128..1104 ] Matching 丨qPCR probe matches [1071·.1098]

GGCTCTTCTTTGCCTCTGCTGGAGTCCGGGGAGTGGCGTTGGCTGCT

AGAGCGATGCCGGGCCGGAGTTGCGTCGCCTTAGTCCTCCTGGCTGCCGC

CGTCAGCTGTGCCGTCGCGCAGCACGCGCCGCCGTGGACAGAGGACTGCA

GAAAATCAACCTATCCTCCTTCAGGACCAACGTACAGAGGTGCAGTTCCA

TGGTACACCATAAATCTTGACTTACCACCCTACAAAAGATGGCATGAATT

GATGCTTGACAAGGCACCAATGCTAAAGGTTATAGTGAATTCTCTGAAGA

ATATGATAAATACATTCGTGCCAAGTGGAAAAGTTATGCAGGTGGTGGAT

GAAAAATTGCCTGGCCTACTTGGCAACTTTCCTGGCCCTTTTGAAGAGGAA 95238.doc -52- 1327645

ATGAAGGGTATTGCCGCTGTTACTGATATACCTTTAGGAGAGATTATTTCA

TTCAATATTTTTTATGAATTATTTACCATTTGTACTTCAATAGTAGCAGAA

GACAAAAAAGGTCATCTAATACATGGGAGAAACATGGATTTTGGAGTATT

TCTTGGGTGGAACATAAATAATGATACCTGGGTCATAACTGAGCAACTAA

AACCTTTAACAGTGAATTTGGATTTCCAAAGAAACAACAAAACTGTCTTC

AAGGCTTCAAGCTTTGCTGGCTATGTGGGCATGTTAACAGGATTCAAACC

AGGACTGTTCAGTCTTACACTGAATGAACGTTTCAGTATAAATGGTGGTTA

TCTGGGTATTCTAGAATGGATTCTGGGAAAGAAAGATGCCATGTGGATAG

GGTTCCTCACTAGAACAGTTCTGGAAAATAGCACAAGTTATGAAGAAGCC

AAGAATTTATTGACCAAGACCAAGATATTGGCCCCAGCCTACTTTATCCTG

GGAGGCAACCAGTCTGGGGAAGGTTGTGTGATTACACGAGACAGAAAGG

AATCATTGGATGTATATGAACTCGATGCTAAGCAGGGTAGATGGTATGTG

GTACAAACAAATTATGACCGTTGGAAACATCCCTTCTTCCTTGATGATCGC

AGAACGCCTGCAAAGATGTGTCTGAACCGCACCAGCCAAGAGAATATCTC

ATTTGAAACCATGTATGATGTCCTGTCAACAAAACCTGTCCTCAACAAGCT

GACCGTATACACAACCTTGATAGATGTTACCAAAGGTCAATTCGAAACTT

ACCTGCGGGACTGCCCTGACCCTTGTATAGGTTGGTGAGCACACGTCTGG

CCTACAGAATGCGGCCTCTGAGACATGAAGACACCATCTCCATGTGACCG

AACACTGCAGCTGTCTGACCTTCCAAAGACTAAGACTCGCGGCAGGTTCT

CTTTGAGTCAAAAGCTTGTCTTCGTCCATCTGTTGACAAATGACAGACCTT

TTTTTTTCCCCCATCAGTTGATTTTTCTTATTTACAGATAACTTCTTTAGGG

GAAGTAAAACAGTCATCTAGAATTCACTGAGTTTTGTTTCACTTTGACATT

TGGGGATCTGGTGGGCAGTCGAACCATGGTGAACTCCACCTCCGTGGAAT

AAATGGAGATTCAGCGTGGGTGTTGAATCCAGCACGTCTGTGTGAGTAAC

GGGACAGTAAACACTCCACATTCTTCAGTTTTTCACTTCTACCTACATATT

TGTATGTTTTTCTGTATAACAGCCTTTTCCTTCTGGTTCTAACTGCTGTTAA

AATTAATATATCATTATCTTTGCTGTTATTGACAGCGATATAATTTTATTAC

ATATGATTAGAGGGATGAGACAGACATTCACCTGTATATTTCTTTTAATGG

GCACAAAATGGGCCCTTGCCTCTAAATAGCACTTTTTGGGGTTCAAGAAG

TAATCAGTATGCAAAGCAATCTTTTATACAATAATTGAAGTGTTCCCTTTT

TCATAATTACTGTACTTCCCAGTAACCCTAAGGAAGTTGCTAACTTAAAAA

ACTGCATCCCACGTTCTGTTAATTTAGTAAATAAACAAGTCAAAGACTTGT

GGAAAATAGGAAGTGAACCCATATTTTAAATTCTCATAAGTAGCATTCAT

GTAATAAACAGGTTTTTAGTTTGTTCTTCAGATTGATAGGGAGTTTTAAAG

AAATTTTAGTAGTTACTAAAATTATGTTACTGTATTTTTCAGAAATCAAAC

TGCTTATGAAAAGTACTAATAGAACTTGTTAACCTTTCTAACCTTCACGAT

TAACTGTGAAATGTACGTCATTTGTGCAAGACCGTTTGTCCACTTCATTTT

GTATAATCACAGTTGTGTTCCTGACACTCAATAAACAGTCATTGGAAAGA GTGCCAGTCAGCAGTCATGCA Sequence No. 87 Protease, Serine 11 >gi | 21327712 | ref | NM-002775.2 | Human Protease, Silk 95238.doc -53- 1327645 Amino Acid 11 (IGF Binding) (PRSS11), mRNA丨qPCR Advance _Introduction matches [1030..1048] I qPCR Reverse-introduction matches [1106..1083] I qPCR probe matches [1080.. 1050]

CCGGCCCTCGCCCTGTCCGCCGCCACCGCCGCCGCCGCCAGAGTCG

CCATGCAGATCCCGCGCGCCGCTCTTCTCCCGCTGCTGCTGCTGCTGCTGG

CGGCGCCCGCCTCGGCGCAGCTGTCCCGGGCCGGCCGCTCGGCGCCTTTG

GCCGCCGGGTGCCCAGACCGCTGCGAGCCGGCGCGCTGCCCGCCGCAGCC

GGAGCACTGCGAGGGCGGCCGGGCCCGGGACGCGTGCGGCTGCTGCGAG

GTGTGCGGCGCGCCCGAGGGCGCCGCGTGCGGCCTGCAGGAGGGCCCGTG

CGGCGAGGGGCTGCAGTGCGTGGTGCCCTTCGGGGTGCCAGCCTCGGCCA

CGGTGCGGCGGCGCGCGCAGGCCGGCCTCTGTGTGTGCGCCAGCAGCGAG

CCGGTGTGCGGCAGCGACGCCAACACCTACGCCAACCTGTGCCAGCTGCG

CGCCGCCAGCCGCCGCTCCGAGAGGCTGCACCGGCCGCCGGTCATCGTCC

TGCAGCGCGGAGCCTGCGGCCAAGGGCAGGAAGATCCCAACAGTTTGCGC

CATAAATATAACTTTATCGCGGACGTGGTGGAGAAGATCGCCCCTGCCGT

GGTTCATATCGAATTGTTTCGCAAGCTTCCGTTTTCTAAACGAGAGGTGCC

GGTGGCTAGTGGGTCTGGGTTTATTGTGTCGGAAGATGGACTGATCGTGA

CAAATGCCCACGTGGTGACCAACAAGCACCGGGTCAAAGTTGAGCTGAAG

AACGGTGCCACTTACGAAGCCAAAATCAAGGATGTGGATGAGAAAGCAG

ACATCGCACTCATCAAAATTGACCACCAGGGCAAGCTGCCTGTCCTGCTG

CTTGGCCGCTCCTCAGAGCTGCGGCCGGGAGAGTTCGTGGTCGCCATCGG

AAGCCCGTTTTCCCTTCAAAACACAGTCACCACCGGGATCGTGAGCACCA

CCCAGCGAGGCGGCAAAGAGCTGGGGCTCCGCAACTCAGACATGGACTA

CATCCAGACCGACGCCATCATCAACTATGGAAACTCGGGAGGCCCGTTAG

TAAACCTGGACGGTGAAGTGATTGGAATTAACACTTTGAAAGTGACAGCT

GGAATCTCCTTTGCAATCCCATCTGATAAGATTAAAAAGTTCCTCACGGAG

TCCCATGACCGACAGGCCAAAGGAAAAGCCATCACCAAGAAGAAGTATA

TTGGTATCCGAATGATGTCACTCACGTCCAGCAAAGCCAAAGAGCTGAAG

GACCGGCACCGGGACTTCCCAGACGTGATCTCAGGAGCGTATATAATTGA

AGTAATTCCTGATACCCCAGCAGAAGCTGGTGGTCTCAAGGAAAACGACG

TCATAATCAGCATCAATGGACAGTCCGTGGTCTCCGCCAATGATGTCAGC

GACGTCATTAAAAGGGAAAGCACCCTGAACATGGTGGTCCGCAGGGGTA

ATGAAGATATCATGATCACAGTGATTCCCGAAGAAATTGACCCATAGGCA

GAGGCATGAGCTGGACTTCATGTTTCCCTCAAAGACTCTCCCGTGGATGAC

GGATGAGGACTCTGGGCTGCTGGAATAGGACACTCAAGACTTTTGACTGC

CATTTTGTTTGTTCAGTGGAGACTCCCTGGCCAACAGAATCCTTCTTGATA

GTTTGCAGGCAAAACAAATGTAATGTTGCAGATCCGCAGGCAGAAGCTCT

GCCCTTCTGTATCCTATGTATGCAGTGTGCTTTTTCTTGCCAGCTTGGGCCA

TTCTTGCTTAGACAGTCAGCATTTGTCTCCTCCTTTAACTGAGTCATCATCT

TAGTCCAACTAATGCAGTCGATACAATGCGTAGATAGAAGAAGCCCCACG 95238.doc -54- 1327645 GGAGCCAGGATGGGACTGGTCGTGTTTGTGCTTTTCTCCAAGTCAGCACCC AAAGGTCAATGCACAGAGACCCCGGGTGGGTGAGCGCTGGCTTCTCAAAC GGCCGAAGTTGCCTCTTTTAGGAATCTCTTTGGAATTGGGAGCACGATGAC TCTGAGTTTGAGCTATTAAAGTACTTCTTACACATTG Sequence No. 88 Secretory Curl-related Protein 2

>gi | 42656988 | ref | XM_050625.4 | Human Secretory Curl-Related Protein 2 (SFRP2), mRNA | qPCR Advance_Introduction and [686..703]才目西己| 9?〇尺逆_弓| Subsequent to [750..728] | qPCR probe matches [705..726]

CCGGGTCGGAGCCCCCCGGAGCTGCGCGCGGGCTTGCAGCGCCTCG CCCGCGCTGTCCTCCCGGTGTCCCGCTTCTCCGCGCCCCAGCCGCCGGCTG CCAGCTTTTCGGGGCCCCGAGTCGCACCCAGCGAAGAGAGCGGGCCCGGG ACAAGCTCGAACTCCGGCCGCCTCGCCCTTCCCCGGCTCCGCTCCCTCTGC CCCCTCGGGGTCGCGCGCCCACGATGCTGCAGGGCCCTGGCTCGCTGCTG CTGCTCTTCCTCGCCTCGCACTGCTGCCTGGGCTCGGCGCGCGGGCTCTTC CTCTTTGGCCAGCCCGACTTCTCCTACAAGCGCAGCAATTGCAAGCCCATC CCTGCCAACCTGCAGCTGTGCCACGGCATCGAATACCAGAACATGCGGCT GCCCAACCTGCTGGGCCACGAGACCATGAAGGAGGTGCTGGAGCAGGCC GGCGCTTGGATCCCGCTGGTCATGAAGCAGTGCCACCCGGACACCAAGAA GTTCCTGTGCTCGCTCTTCGCCCCCGTCTGCCTCGATGACCTAGACGAGAC CATCCAGCCATGCCACTCGCTCTGCGTGCAGGTGAAGGACCGCTGCGCCC CGGTCATGTCCGCCTTCGGCTTCCCCTGGCCCGACATGCTTGAGTGCGACC GTTTCCCCCAGGACAACGACCTTTGCATCCCCCTCGCTAGCAGCGACCACC TCCTGCCAGCCACCGAGGAAGCTCCAAAGGTATGTGAAGCCTGCAAAAAT AAAAATGATGATGACAACGACATAATGGAAACGCTTTGTAAAAATGATTT TGCACTGAAAATAAAAGTGAAGGAGATAACCTACATCAACCGAGATACC AAAATCATCCTGGAGACCAAGAGCAAGACCATTTACAAGCTGAACGGTGT GTCCGAAAGGGACCTGAAGAAATCGGTGCTGTGGCTCAAAGACAGCTTGC AGTGCACCTGTGAGGAGATGAACGACATCA ACGCGCCCTATCTGGTCATG GGACAGAAACAGGGTGGGGAGCTGGTGATCACCTCGGTGAAGCGGTGGC AGAAGGGGCAGAGAGAGTTCAAGCGCATCTCCCGCAGCATCCGCAAGCT GCAGTGCTAGTCCCGGCATCCTGATGGCTCCGACAGGCCTGCTCCAGAGC ACGGCTGACCATTTCTGCTCCGGGATCTCAGCTCCCGTTCCCCAAGCACAC TCCTAGCTGCTCCAGTCTCAGCCTGGGCAGCTTCCCCCTGCCTTTTGCACG TTTGCATCCCCAGCATTTCCTGAGTTATAAGGCCACAGGAGTGGATAGCTG TTTTCACCTAAAGGAAAAGCCCACCCGAATCTTGTAGAAATATTCAAACT AATAAAATCATGAATATTTTTATGAAGTTTAAAAA serial No. 89 95238.doc 55 · 1327645 phospholipase A2, XIIB group

>gi 丨 45505134 丨 ref I NM—032562.2 I Human phospholipase A2, XIIB group (PLA2G12B), mRNA

TGTCCCTGGAATTCTGGGACACTGGCTGGGGTTTGAGGAGAGAAGC

CAGTACCTACCTGGCTGCAGGATGAAGCTGGCCAGTGGCTTCTTGGTTTTG

TGGCTCAGCCTTGGGGGTGGCCTGGCTCAGAGCGACACGAGCCCTGACAC

GGAGGAGTCCTATTCAGACTGGGGCCTTCGGCACCTCCGGGGAAGCTTTG

AATCCGTCAATAGCTACTTCGATTCTTTTCTGGAGCTGCTGGGAGGGAAGA

ATGGAGTCTGTCAGTACAGGTGCCGATATGGAAAGGCACCAATGCCCAGA

CCTGGCTACAAGCCCCAAGAGCCCAATGGCTGCGGCTCCTATTTCCTGGGT ctcaaggtaccagaaagtatggacttgggcattccagcaatgacaaagtg

CTGCAACCAGCTGGATGTCTGTTATGACACTTGCGGTGCCAACAAATATC GCTGTGATGCAAAATTCCGATGGTGTCTCCACTCGATCTGCTCTGACCTTA AGCGGAGTCTGGGCTTTGTCTCCAAAGTGGAAGCAGCCTGTGATTCCCTG GTTGACACTGTGTTCAACACCGTGTGGACCTTGGGCTGCCGCCCCTTTATG AATAGTCAGCGGGCAGCTTGCATCTGTGCAGAGGAGGAGAAGGAAGAGT TATGAGGAAGAAGTGATTCCTTCCTGGTTTTGAGTGACACCACAGCTGTCA GCCTTCAAGATGTCAAGTCTTCGAGTCAGCGTGACTCATTCATTCTTCCAA CAGTTTGGACACCACAAAGCAGGAGAAAGGGAACATTTTTCTACAGCTGG AAAGTGAGTCCTATCCTTTGAGGAAATTTGAAAAAAGACATGGAGTGGTT TGAAAGCTACTCTTCATTTAAGACTGCTCTCCCCAACCAAGACACATTTGC CTGGAAATTCAGTTCTTAGCTTAAAGACTAAAATGCAAGCAAACCCTGCA ATTCCTGGACCTGATAGTTATATTCATGAGTGAAATTGTGGGGAGTCCAGC CATTTGGGAGGCAATGACTTTCTGCTGGCCCATGTTTCAGTTGCCAGTAAG CTTCTCACATTTAATAAAGTGTACTTTTTAGAACATT next serial No. 90 Si 2 Ting, extracellular matrix proteins

>gi | 6912681 | ref | ΝΜ_012445.1 | Human statin 2, extracellular matrix protein (SPON2), mRNA

GCACGAGGGAAGAGGGTGATCCGACCCGGGGAAGGTCGCTGGGCA

GGGCGAGTTGGGAAAGCGGCAGCCCCCGCCGCCCCCGCAGCCCCTTCTCC

TCCTTTCTCCCACGTCCTATCTGCCTCTCGCTGGAGGCCAGGCCGTGCAGC

ATCGAAGACAGGAGGAACTGGAGCCTCATTGGCCGGCCCGGGGCGCCGG

CCTCGGGCTTAAATAGGAGCTCCGGGCTCTGGCTGGGACCCGACCGCTGC

CGGCCGCGCTCCCGCTGCTCCTGCCGGGTGATGGAAAACCCCAGCCCGGC

CGCCGCCCTGGGCAAGGCCCTCTGCGCTCTCCTCCTGGCCACTCTCGGCGC

CGCCGGCCAGCCTCTTGGGGGAGAGTCCATCTGTTCCGCCAGAGCCCCGG

CCAAATACAGCATCACCTTCACGGGCAAGTGGAGCCAGACGGCCTTCCCC 95238.doc -56- 1327645

AAGCAGTACCCCCTGTTCCGCCCCCCTGCGCAGTGGTCTTCGCTGCTGGGG GCCGCGCATAGCTCCGACTACAGCATGTGGAGGAAGAACCAGTACGTCAG TAACGGGCTGCGCGACTTTGCGGAGCGCGGCGAGGCCTGGGCGCTGATGA AGGAGATCGAGGCGGCGGGGGAGGCGCTGCAGAGCGTGCACGCGGTGTT TTCGGCGCCCGCCGTCCCCAGCGGCACCGGGCAGACGTCGGCGGAGCTGG AGGTGCAGCGCAGGCACTCGCTGGTCTCGTTTGTGGTGCGCATCGTGCCC AGCCCCGACTGGTTCGTGGGCGTGGACAGCCTGGACCTGTGCGACGGGGA CCGTTGGCGGGAACAGGCGGCGCTGGACCTGTACCCCTACGACGCCGGGA CGGACAGCGGCTTCACCTTCTCCTCCCC-CAACTTCGCCACCATCCCGCAGG ACACGGTGACCGAGATAACGTCCTCCTCTCCCAGCCACCCGGCCAACTCC TTCTACTACCCGCGGCTGAAGGCCCTGCCTCCCATCGCCAGGGTGACACTG GTGCGGCTGCGACAGAGCCCCAGGGCCTTCATCCCTCCCGCCCCAGTCCT GCCCAGCAGGGACAATGAGATTGTAGACAGCGCCTCAGTTCCAGAAACGC CGCTGGACTGCGAGGTCTCCCTGTGGTCGTCCTGGGGACTGTGCGGAGGC CACTGTGGGAGGCTCGGGACCAAGAGCAGGACTCGCTACGTCCGGGTCCA GCCCGCCAACAACGGGAGCCCCTGCCCCGAGCTCGAAGAAGAGGCTGAG TGCGTCCCTGATAACTGCGTCTAAGACCAGAGCCCCGCAGCCCCTGGGGC CCCCGGAGCCATGGGGTGTCGGGGGCTCCTGTGCAGGCTCATGCTGCAGG CGGCCGAGGCACAGGGGGTTTCGCGCTGCTCCTGACCGCGGTGAGGCCGC GCCGACCATCTCTGCACTGAAGGGCCC TCTGGTGGCCGGCACGGGCATTG GGAAACAGCCTCCTCCTTTCCCAACCTTGCTTCTTAGGGGCCCCCGTGTCC CGTCTGCTCTCAGCCTCCTCCTCCTGCAGGATAAAGTCATCCCCAAGGCTC CAGCTACTCTAAATTATGGTCTCCTTATAAGTTATTGCTGCTCCAGGAGAT TGTCCTTCATCGTCCAGGGGCCTGGCTCCCACGTGGTTGCAGATACCTCAG ACCTGGTGCTCTAGGCTGTGCTGAGCCCACTCTCCCGAGGGCGCATCCAA GCGGGGGCCACTTGAGAAGTGAATAAATGGGGCGGTTTCGGAAGCGTCA GTGTTTCCATGTTATGGATCTCTCTGCGTTTGAATAAAGACTATCTCTGTTG CTCAC serial No. 91 torr US Olfactory set 1, transcript variant 3

>gi | 34335282 | ref | NM_058199.2 | Human Bromoxetine l (OLFM1), Transcriptional Variant 3, mRNA

CCCGCCCCCGCCCCTTCCGAGCAAACTTTTGGCACCCACCGCAGCC

CAGCGCGCGTTCGTGCTCCGCAGGGCGCGCCTCTCTCCGCCAATGCCAGG

CGCGCGGGGGAGCCATTAGGAGGCGAGGAGAGAGGAGGGCGCAGCTCCC

GCCCAGCCCAGCCCTGCCCAGCCCTGCCCGGAGGCAGACGCGCCGGAACC

GGGACGCGATAAATATGCAGAGCGGAGGCTTCGCGCAGCAGAGCCCGCG

CGCCGCCCGCTCCGGGTGCTGAATCCAGGCGTGGGGACACGAGCCAGGCG

CCGCCGCCGGAGCCAGCGGAGCCGGGGCCAGAGCCGGAGCGCGTCCGCG

TCCACGCAGCCGCCGGCCGGCCAGCACCCAGGGCCCTGCATGCCAGGTCG

TTGGAGGTGGCAGCGAGACATGCACCCGGCCCGGAAGCTCCTCAGCCTCC 95238.doc • 57- 1327645 TCTTCCTCATCCTGATGGGCACTGAACTCACTCAAAATAAAAGAGAAAAC AAAGCAGAGAAGATGGGAGGGCCAGAGAGCGAGAGGAAGACCACAGGA GAGAAGACACTGAACGAGCTTCCCTTGTTTTGCCTGGAAGCCCACGCTGG CTCCCTGGCTCTGCCCAGGATGTGCAGTCCAAATCCCAATCCAGCAGTGG GGTTATGTCGTCCCGCTTACCCTCAGAGCCCTTCTCCTGGTGCTGCCCAGA CGATCAGCCAGTCCCTCCTGGAGAGGTTCTGCATGGCCTCTAGGAGAGAA GTTTTCTTGGCCCCAGGAAGGCCTGGTGGAGGGTGGTGGTTGTGCACTGTT GCTGGACAGATGCATTCATTCATGTGCACACACACACACACACATGCACA CACAGGGGAGCAGATACCTGCAGAGAAGAGCCAACCAGGTCCTGATTAG TGGCAAGCTGCCCCACAAAGGGCTATGCCTGTGTCTTATTGAGACACCTTG GCAAAGAGATGGCTGATTCTGGGTGGTCCTGGACATGGCCGCACCCAAGG GCCCTCCAAGCCTTAATGGCACCCTGAAGCCTCCATGCCCAGGCCAAAAG ATGCTTTTCCTCCCTAAAAAAAAAAAAAAAAAAA sequence bad, J No. 92 containing the platelet-reactive protein repeats Lu 1 > gi | 38016903 | ref | NM_019032.2 | human Small blood containing 1

Plate reactive protein repeat (TSRC1), mRNA

GGGGCCCCAGTGGCCGCCGCGGAGCGAGGTTGCCTGGAGAGAGCG

CCTGGGCGCAGAAGGGTTAACGGGCCACCGGGGGCTCGCAGAGCAGGAG

GGTGCTCTCGGACGGTGTGTCCCCCACTGCACTCCTGAACTTGGAGGACA

GGGTCGCCGCGAGGGACGCAGAGAGCACCCTCCACGCCCAGATGCCTGCG

TAGTTTTTGTGACCAGTCCGCTCCTGCCTCCCCCTGGGGCAGTAGAGGGGG

AGCGATGGAGAACTGGACTGGCAGGCCCTGGCTGTATCTGCTGCTGCTTC

TGTCCCTCCCTCAGCTCTGCTTGGATCAGGAGGTGTTGTCCGGACACTCTC

TTCAGACACCTACAGAGGAGGGCCAGGGCCCCGAAGGTGTCTGGGGACCT

TGGGTCCAGTGGGCCTCTTGCTCCCAGCCCTGCGGGGTGGGGGTGCAGCG

CAGGAGCCGGACATGTCAGCTCCCTACAGTGCAGCTCCACCCGAGTCTGC

CCCTCCCTCCCCGGCCCCCAAGACATCCAGAAGCCCTCCTCCCCCGGGGCC

AGGGTCCCAGACCCCAGACTTCTCCAGAAACCCTCCCCTTGTACAGGACA

CAGTCTCGGGGAAGGGGTGGCCCACTTCGAGGTCCCGCTTCCCACCTAGG

GAGAGAGGAGACCCAGGAGATTCGAGCGGCCAGGAGGTCCCGGCTTCGA

GACCCCATCAAGCCAGGAATGTTCGGTTATGGGAGAGTGCCCTTTGCATT

GCCACTGCACCGGAACCGCAGGCACCCTCGGAGCCCACCCAGATCTGAGC

TGTCCCTGATCTCTTCTAGAGGGGAAGAGGCTATTCCGTCCCCTACTCCAA

GAGCAGAGCCATTCTCCGCAAACGGCAGCCCCCAAACTGAGCTCCCTCCC

ACAGAACTGTCTGTCCACACCCCATCCCCCCAAGCAGAACCTCTAAGCCC

TGAAACTGCTCAGACAGAGGTGGCCCCCAGAACCAGGCCTGCCCCCCTAC

GGCATCACCCCAGAGCCCAGGCCTCTGGCACAGAGCCCCCCTCACCCACG

CACTCCTTAGGAGAAGGTGGCTTCTTCCGTGCATCCCCTCAGCCACGAAG

GCCAAGTTCCCAGGGTTGGGCCAGTCCCCAGGTAGCAGGGAGACGCCCTG

ATCCTTTTCCTTCGGTCCCTCGGGGCCGAGGCCAGCAGGGCCAAGGGCCTT 95238.doc -58- 1327645

GGGGAACGGGGGGGACTCCTCACGGGCCCCGCCTGGAGCCTGACCCTCAG

CACCCGGGCGCCTGGCTGCCCCTGCTGAGCAACGGCCCCCATGCCAGCTC

CCTCTGGAGCCTCTTTGCTCCCAGTAGCCCTATTCCAAGATGTTCTGGGGA

GAGTGAACAGCTAAGAGCCTGCAGCCAAGCGCCCTGCCCCCCTGAGCAGC

CAGACCCCCGGGCCCTGCAGTGCGCAGCCTTTAACTCCCAGGAATTCATG

GGCCAGCTGTATCAGTGGGAGCCCTTCACTGAAGTCCAGGGCTCCCAGCG

CTGTGAACTGAACTGCCGGCCCCGTGGCTTCCGCTTCTATGTCCGTCACAC

TGAAAAGGTCCAGGATGGGACCCTGTGTCAGCCTGGAGCCCCTGACATCT

GTGTGGCTGGACGCTGTCTGAGCCCCGGCTGTGATGGGATCCTTGGCTCTG

GCAGGCGTCCTGATGGCTGTGGAGTCTGTGGGGGTGATGATTCTACCTGTC

GCCTTGTTTCGGGGAACCTCACTGACCGAGGGGGCCCCCTGGGCTATCAG

AAGATCTTGTGGATTCCAGCGGGAGCCTTGCGGCTCCAGATTGCCCAGCT

CCGGCCTAGCTCCAACTACCTGGCACTTCGTGGCCCTGGGGGCCGGTCCAT

CATCAATGGGAACTGGGCTGTGGATCCCCCTGGGTCCTACAGGGCCGGCG

GGACCGTCTTTCGATATAACCGTCCTCCCAGGGAGGAGGGCAAAGGGGAG

AGTCTGTCGGCTGAAGGCCCCACCACCCAGCCTGTGGATGTCTATATGATC

TTTCAGGAGGAAAACCCAGGCGTTTTTTATCAGTATGTCATCTCTTCACCT

CCTCCAATCCTTGAGAACCCCACCCCAGAGCCCCCTGTCCCCCAGCTTCAG

CCGGAGATTCTGAGGGTGGAGCCCCCACTTGCTCCGGCACCCCGCCCAGC

CCGGACCCCAGGCACCCTCCAGCGTCAGGTGCGGATCCCCCAGATGCCCG

CCCCGCCCCATCCCAGGACACCCCTGGGGTCTCCAGCTGCGTACTGGAAA

CGAGTGGGACACTCTGCATGCTCAGCGTCCTGCGGGAAAGGTGTCTGGCG

CCCCATTTTCCTCTGCATCTCCCGTGAGTCGGGAGAGGAACTGGATGAAC

GCAGCTGTGCCGCGGGTGCCAGGCCCCCAGCCTCCCCTGAACCCTGCCAC

GGCACCCCATGCCCCCCATACTGGGAGGCTGGCGAGTGGACATCCTGCAG

CCGCTCCTGTGGCCCCGGCACCCAGCACCGCCAGCTGCAGTGCCGGCAGG

AATTTGGGGGGGGTGGCTCCTCGGTGCCCCCGGAGCGCTGTGGACATCTC

CCCCGGCCCAACATCACCCAGTCTTGCCAGCTGCGCCTCTGTGGCCATTGG

GAAGTTGGCTCTCCTTGGAGCCAGTGCTCCGTGCGGTGCGGCCGGGGCCA

GAGAAGCCGGCAGGTTCGCTGTGTTGGGAACAACGGTGATGAAGTGAGC

GAGCAGGAGTGTGCGTCAGGCCCCCCGCAGCCCCCCAGCAGAGAGGCCTG

TGACATGGGGCCCTGTACTACTGCCTGGTTCCACAGCGACTGGAGCTCCA

AGTGCTCAGCCGAGTGTGGGACGGGAATCCAGCGGCGCTCTGTGGTCTGC

CTTGGGAGTGGGGCAGCCCTCGGGCCAGGCCAGGGGGAAGCAGGAGCAG

GAACTGGGCAGAGCTGTCCAACAGGAAGCCGGCCCCCTGACATGCGCGCC

TGCAGCCTGGGGCCCTGTGAGAGAACTTGGCGCTGGTACACAGGGCCCTG

GGGTGAGTGCTCCTCCGAATGTGGCTCTGGCACACAGCGTAGAGACATCA

TCTGTGTATCCAAACTGGGGACGGAGTTCAACGTGACTTCTCCGAGCAAC

TGTTCTCACCTCCCCAGGCCCCCTGCCCTGCAGCCCTGTCAAGGGCAGGCC

TGCCAGGACCGATGGTTTTCCACGCCCTGGAGCCCATGTTCTCGCTCCTGC

CAAGGGGGAACGCAGACACGGGAGGTCCAGTGCCTGAGCACCAACCAGA

CCCTCAGCACCCGATGCCCTCCTCAACTGCGGCCCTCCAGGAAGCGCCCCT

GTAACAGCCAACCCTGCAGCCAGCGCCCTGATGATCAATGCAAGGACAGC 95238.doc •59- 1327645

TCTCCACATTGCCCCCTGGTGGTACAGGCCCGGCTCTGCGTCTACCCCTAC TACACAGCCACCTGTTGCCGCTCTTGCGCACATGTCCTGGAGCGGTCTCCC CAGGATCCCTCCTGAAAGGGGTCCGGGGCACCTTCACGGTTTTCTGTGCCA CCATCGGTCACCCATTGATCGGCCCACTCTGAACCCCCTGGCTCTCCAGCC TGTCCCAGTCTCAGCAGGGATGTCCTCCAGGTGACAGAGGGTGGCAAGGT GACTGACACAAAGTGACTTTCAGGGCTGTGGTCAGGCCCATGTGGTGGTG TGATGGGTGTGTGCACATATGCCTCAGGTGTGCTTTTGGGACTGCATGGAT ATGTGTGTGCTCAAACGTGTATCACTTTTCAAAAAGAGGTTACACAGACT GAGAAGGACAAGACCTGTTTCCTTGAGACTTTCCTAGGTGGAAAGGAAAG CAAGTCTGCAGTTCCTTGCTAATCTGAGCTACTTAGAGTGTGGTCTCCCCA CCAACTCCAGTTTTGTGCCCTAAGCCTCATTTCTCATGTTCAGACCTCACA TCTTCTAAGCCGCCCTGTGTCTCTGACCCCTTCTCATTTGCCTAGTATCTCT GCCCCTGCCTCCCTAATTAGCTAGGGCTGGGGTCAGCCACTGCCAATCCTG CCTTACTCAGGAAGGCAGGAGGAAAGAGACTGCCTCTCCAGAGCAAGGC CCAGCTGGGCAGAGGGTGAAAAAGAGAAATGTGAGCATCCGCTCCCCCA CCACCCCGCCCAGCCCCTAGCCCCACTCCCTGCCTCCTGAAATGGTTCCCA CCCAGAACTAATTTATTTTTTATTAAAGATGGTCATGACAAATGAAAAAA AAAAAAAAAAAAA Serial No. 93 Human thrombospondin 2 > gi | 40317627 | ref | NM_003247.2 | human anti-human platelets Protein 2 (THBS2), mRNA | qPCR advance_bow|match with [3558·.3580] | qPCR reverse _ primer matches [3682..3655] | qPCR probe matches [3597·, 3623]

GAGGAGGAGACGGCATCCAGTACAGAGGGGCTGGACTTGGACCCC

TGCAGCAGCCCTGCACAGGAGAAGCGGCATATAAAGCCGCGCTGCCCGG

GAGCCGCTCGGCCACGTCCACCGGAGCATCCTGCACTGCAGGGCCGGTCT

CTCGCTCCAGCAGAGCCTGCGCCTTTCTGACTCGGTCCGGAACACTGAAA

CCAGTCATCACTGCATCTTTTTGGCAAACCAGGAGCTCAGCTGCAGGAGG

CAGGATGGTCTGGAGGCTGGTCCTGCTGGCTCTGTGGGTGTGGCCCAGCA

CGCAAGCTGGTCACCAGGACAAAGACACGACCTTCGACCTTTTCAGTATC

AGCAACATCAACCGCAAGACCATTGGCGCCAAGCAGTTCCGCGGGCCCGA

CCCCGGCGTGCCGGCTTACCGCTTCGTGCGCTTTGACTACATCCCACCGGT

GAACGCAGATGACCTCAGCAAGATCACCAAGATCATGCGGCAGAAGGAG

GGCTTCTTCCTCACGGCCCAGCTCAAGCAGGACGGCAAGTCCAGGGGCAC

GCTGTTGGCTCTGGAGGGCCCCGGTCTCTCCCAGAGGCAGTTCGAGATCG

TCTCCAACGGCCCCGCGGACACGCTGGATCTCACCTACTGGATTGACGGC

ACCCGGCATGTGGTCTCCCTGGAGGACGTCGGCCTGGCTGACTCGCAGTG

GAAGAACGTCACCGTGCAGGTGGCTGGCGAGACCTACAGCTTGCACGTGG 95238.doc -60- 1327645

GCTGCGACCTCATAGACAGCTTCGCTCTGGACGAGCCCTTCTACGAGCAC

CTGCAGGCGGAAAAGAGCCGGATGTACGTGGCCAAAGGCTCTGCCAGAG

AGAGTCACTTCAGGGGTTTGCTTCAGAACGTCCACCTAGTGTTTGAAAACT

CTGTGGAAGATATTCTAAGCAAGAAGGGTTGCCAGCAAGGCCAGGGAGCT

GAGATCAACGCCATCAGTGAGAACACAGAGACGCTGCGCCTGGGTCCGCA

TGTCACCACCGAGTACGTGGGCCCCAGCTCGGAGAGGAGGCCCGAGGTGT

GCGAACGCTCGTGCGAGGAGCTGGGAAACATGGTCCAGGAGCTCTCGGG

GCTCCACGTCCTCGTGAACCAGCTCAGCGAGAACCTCAAGAGAGTGTCGA

ATGATAACCAGTTTCTCTGGGAGCTCATTGGTGGCCCTCCTAAGACAAGG

AACATGTCAGCTTGCTGGCAGGATGGCCGGTTCTTTGCGGAAAATGAAAC

GTGGGTGGTGGACAGCTGCACCACGTGTACCTGCAAGAAATTTAAAACCA

TTTGCCACCAAATCACCTGCCCGCCTGCAACCTGCGCCAGTCCATCCTTTG

TGGAAGGCGAATGCTGCCCTTCCTGCCTCCACTCGGTGGACGGTGAGGAG

GGCTGGTCTCCGTGGGCAGAGTGGACCCAGTGCTCCGTGACGTGTGGCTC

TGGGACCCAGCAGAGAGGCCGGTCCTGTGACGTCACCAGCAACACCTGCT

TGGGGCCCTCCATCCAGACACGGGCTTGCAGTCTGAGCAAGTGTGACACC

CGCATCCGGCAGGACGGCGGCTGGAGCCACTGGTCACCTTGGTCTTCATG

CTCTGTGACCTGTGGAGTTGGCAATATCACACGCATCCGTCTCTGCAACTC

CCCAGTGCCCCAGATGGGGGGCAAGAATTGCAAAGGGAGTGGCCGGGAG

ACCAAAGCCTGCCAGGGCGCCCCATGCCCAATCGATGGCCGCTGGAGCCC

CTGGTCCCCGTGGTCGGCCTGCACTGTCACCTGTGCCGGTGGGATCCGGG

AGCGCACCCGGGTCTGCAACAGCCCTGAGCCTCAGTACGGAGGGAAGGCC

TGCGTGGGGGATGTGCAGGAGCGTCAGATGTGCAACAAGAGGAGCTGCC

CCGTGGATGGCTGTTTATCCAACCCCTGCTTCCCGGGAGCCCAGTGCAGCA

GCTTCCCCGATGGGTCCTGGTCATGCGGCTCCTGCCCTGTGGGCTTCTTGG

GCAATGGCACCCACTGTGAGGACCTGGACGAGTGTGCCCTGGTCCCCGAC

ATCTGCTTCTCCACCAGCAAGGTGCCTCGCTGTGTCAACACTCAGCCTGGC

TTCCACTGCCTGCCCTGCCCGCCCCGATACAGAGGGAACCAGCCCGTCGG

GGTCGGCCTGGAAGCAGCCAAGACGGAAAAGCAAGTGTGTGAGCCCGAA

AACCCATGCAAGGACAAGACACACAACTGCCACAAGCACGCGGAGTGCA

TCTACCTGGGCCACTTCAGCGACCCCATGTACAAGTGCGAGTGCCAGACA

GGCTACGCGGGCGACGGGCTCATCTGCGGGGAGGACTCGGACCTGGACG

GCTGGCCCAACCTCAATCTGGTCTGCGCCACCAACGCCACCTACCACTGC

ATCAAGGATAACTGCCCCCATCTGCCAAATTCTGGGCAGGAAGACTTTGA

CAAGGACGGGATTGGCGATGCCTGTGATGATGACGATGACAATGACGGTG

TGACCGATGAGAAGGACAACTGCCAGCTCCTCTTCAATCCCCGCCAGGCT

GACTATGACAAGGATGAGGTTGGGGACCGCTGTGACAACTGCCCTTACGT

GCACAACCCTGCCCAGATCGACACAGACAACAATGGAGAGGGTGACGCC

TGCTCCGTGGACATTGATGGGGACGATGTCTTCAATGAACGAGACAATTG

TCCCTACGTCTACAACACTGACCAGAGGGACACGGATGGTGACGGTGTGG

GGGATCACTGTGACAACTGCCCCCTGGTGCACAACCCTGACCAGACCGAC

GTGGACAATGACCTTGTTGGGGACCAGTGTGACAACAACGAGGACATAGA

TGACGACGGCCACCAGAACAACCAGGACAACTGCCCCTACATCTCCAACG 95238.doc -61 - 1327645

CCAACCAGGCTGACCATGACAGAGACGGCCAGGGCGACGCCTGTGACCCT

GATGATGACAACGATGGCGTCCCCGATGACAGGGACAACTGCCGGCTTGT

GTTCAACCCAGACCAGGAGGACTTGGACGGTGATGGACGGGGTGATATTT

GTAAAGATGATTTTGACAATGACAACATCCCAGATATTGATGATGTGTGT

CCTGAAAACAATGCCATCAGTGAGACAGACTTCAGGAACTTCCAGATGGT

CCCCTTGGATCCCAAAGGGACCACCCAAATTGATCCCAACTGGGTCATTC

GCCATCAAGGCAAGGAGCTGGTTCAGACAGCCAACTCGGACCCCGGCATC

GCTGTAGGTTTTGACGAGTTTGGGTCTGTGGACTTCAGTGGCACATTCTAC

GTAAACACTGACCGGGACGACGACTATGCCGGCTTCGTCTTTGGTTACCA

GTCAAGCAGCCGCTTCTATGTGGTGATGTGGAAGCAGGTGACGCAGACCT

ACTGGGAGGACCAGCCCACGCGGGCCTATGGCTACTCCGGCGTGTCCCTC

AAGGTGGTGAACTCCACCACGGGGACGGGCGAGCACCTGAGGAACGCGC

TGTGGCACACGGGGAACACGCCGGGGCAGGTGCGAACCTTATGGCACGA

CCCCAGGAACATTGGCTGGAAGGACTACACGGCCTATAGGTGGCACCTGA

C-TCACAGGCCCAAGACTGGCTACATCAGAGTCTTAGTGCATGAAGGAAAA

CAGGTCATGGCAGACTCAGGACCTATCTATGACCAAACCTACGCTGGCGG

GCGGCTGGGTCTATTTGTCTTCTCTCAAGAAATGGTCTATTTCTCAGACCT

CAAGTACGAATGCAGAGATATTTAAACAAGATTTGCTGCATTTCCGGCAA

TGCCCTGTGCATGCCATGGTCCCTAGACACCTCAGTTCATTGTGGTCCTTG

TGGCTTCTCTCTCTAGCAGCACCTCCTGTCCCTTGACCTTAACTCTGATGGT

TCTTCACCTCCTGCCAGCAACCCCAAACCCAAGTGCCTTCAGAGGATAAA

TATCAATGGAACTCAGAGATGAACATCTAACCCACTAGAGGAAACCAGTT

TGGTGATATATGAGACTTTATGTGGAGTGAAAATTGGGCATGCCATTACA

TTGCTTTTTCTTGTTTGTTTAAAAAGAATGACGTTTACATATAAAATGTAA

TTACTTATTGTATTTATGTGTATATGGAGTTGAAGGGAATACTGTGCATAA

GCCATTATGATAAATTAAGCATGAAAAATATTGCTGAACTACTTTTGGTGC

TTAAAGTTGTCACTATTCTTGAATTAGAGTTGCTCTACAATGACACACAAA

TCCCATTAAATAAATTATAAACAAGGGTCAATTCAAATTTGAAGTAATGTT

TTAGTAAGGAGAGATTAGAAGACAACAGGCATAGCAAATGACATAAGCT

ACCGATTAACTAATCGGAACATGTAAAACAGTTACAAAAATAAACGAACT

CTCCTCTTGTCCTACAATGAAAGCCCTCATGTGCAGTAGAGATGCAGTTTC

ATCAAAGAACAAACATCCTTGCAAATGGGTGTGACGCGGTTCCAGATGTG

GATTTGGCAAAACCTCATTTAAGTAAAAGGTTAGCAGAGCAAAGTGCGGT

GCTTTAGCTGCTGCTTGTGCCGCTGTGGCGTCGGGGAGGCTCCTGCCTGAG

CTTCCTTCCCCAGCTTTGCTGCCTGAGAGGAACCAGAGCAGACGCACAGG

CCGGAAAAGGCGCATCTAACGCGTATCTAGGCTTTGGTAACTGCGGACAA

GTTGCTTTTACCTGATTTGATGATACATTTCATTAAGGTTCCAGTTATAAAT

ATTTTGTTAATATTTATTAAGTGACTATAGAATGCAACTCCATTTACCAGT

AACTTATTTTAAATATGCCTAGTAACACATATGTAGTATAATTTCTAGAAA

CAAACATCTAATAAGTATATAATCCTGTGAAAATATGAGGCTTGATAATA

TTAGGTTGTCACGATGAAGCATGCTAGAAGCTGTAACAGAATACATAGAG

AATAATGAGGAGTTTATGATGGAACCTTAAATATATAATGTTGCCAGCGA

TTTTAGTTCAATATTTGTTACTGTTATCTATCTGCTGTATATGGAATTCTTT 95238.doc -62- 1327645

TAATTCAAACGCTGAAAAGAATCAGCATTTAGTCTTGCCAGGCACACCCA ATAATCAGTCATGTGTAATATGCACAAGTTTGTTTTTGTTTTTGTTTTTTTT GTTGGTTGGTTTGTTTTTTTGCTTTAAGTTGCATGATCTTTCTGCAGGAAAT AGTCACTCATCCCACTCCACATAAGGGGTTTAGTAAGAGAAGTCTGTCTGT CTGATGATGGATAGGGGGCAAATCTTTTTCCCCTTTCTGTTAATAGTCATC ACATTTCTATGCCAAACAGGAACAATCCATAACTTTAGTCTTAATGTACAC ATTGCATTTTGATAAAATTAATTTTGTTGTTTCCTTTGAGGTTGATCGTTGT GTTGTTGTTTTGCTGCACTTTTTACTTTTTTGCGTGTGGAGCTGTATTCCCG AGACCAACGAAGCGTTGGGATACTTCATTAAATGTAGCGACTGTCAACAG CGTGCAGGTTTTCTGTTTCTGTGTTGTGGGGTCAACCGTACAATGGTGTGG GAGTGACGATGATGTGAATATTTAGAATGTACCATATTTTTTGTAAATTAT TTATGTTTTTCTAAACAAATTTATCGTATAGGTTGATGAAACGTCATGTGT TTTGCCAAAGACTGTAAATATTTATTTATGTGTTCACATGGTCAAAATTTC ACCACTGAAACCCTGCACTTAGCTAGAACCTCATTTTTAAAGATTAACAAC AGGAAATAAATTGTAAAAAAGGTTTTCTATACATGAAAAAAAAAAAAAA AAAA Serial No. 94 Adelaide Milliken > gi I 18390318 I ref Shu NM_0015419.1 I human Adelaide Milliken (DKFZp564I1922), mRNA I qPCR around The text is determined to match [694..718] when requested.

ATGCCCAAGCGCGCGCACTGGGGGGCCCTCTCCGTGGTGCTGATCC

TGCTTTGGGGCCATCCGCGAGTGGCGCTGGCCTGCCCGCATCCTTGTGCCT

GCTACGTCCCCAGCGAGGTCCACTGCACGTTCCGATCCCTGGCTTCCGTGC

CCGCTGGCATTGCTAGACACGTGGAAAGAATCAATTTGGGGTTTAATAGC

ATACAGGCCCTGTCAGAAACCTCATTTGCAGGACTGACCAAGTTGGAGCT

ACTTATGATTCACGGCAATGAGATCCCAAGCATCCCCGATGGAGCTTTAA

GAGACCTCAGCTCTCTTCAGGTTTTCAAGTTCAGCTACAACAAGCTGAGA

GTGATCACAGGACAGACCCTCCAGGGTCTCTCTAACTTAATGAGGCTGCA

CATTGACCACAACAAGATCGAGTTTATCCACCCTCAAGCTTTCAACGGCTT

AACGTCTCTGAGGCTACTCCATTTGGAAGGAAATCTCCTCCACCAGCTGCA

CCCCAGCACCTTCTCCACGTTCACATTTTTGGATTATTTCAGACTCTCCACC

ATAAGGCACCTCTACTTAGCAGAGAACATGGTTAGAACTCTTCCTGCCAG

CATGCTTCGGAACATGCCGCTTCTGGAGAATCTTTACTTGCAGGGAAATCC

GTGGACCTGCGATTGTGAGATGAGATGGTTTTTGGAATGGGATGCAAAAT

CCAGAGGAATTCTGAAGTGTAAAAAGGACAAAGCTTATGAAGGCGGTCA

GTTGTGTGCAATGTGCTTCAGTCCAAAGAAGTTGTACAAACATGAGATAC

ACAAGCTGAAGGACATGACTTGTCTGAAGCCTTCAATAGAGTCCCCTCTG

AGACAGAACAGGAGCAGGAGTATTGAGGAGGAGCAAGAACAGGAAGAG

GATGGTGGCAGCCAGCTCATCCTGGAGAAATTCCAACTGCCCCAGTGGAG 95238.doc -63- 1327645

CATCTCTTTGAATATGACCGACGAGCACGGGAACATGGTGAACTTGGTCT

GTGACATCAAGAAACCAATGGATGTGTACAAGATTCACTTGAACCAAACG

GATCCTCCAGATATTGACATAAATGCAACAGTTGCCTTGGACTTTGAGTGT

CCAATGACCCGAGAAAACTATGAAAAGCTATGGAAATTGATAGCATACTA

CAGTGAAGTTCCCGTGAAGCTACACAGAGAGCTCATGCTCAGCAAAGACC

CCAGAGTCAGCTACCAGTACAGGCAGGATGCTGATGAGGAAGCTCTTTAC

TACACAGGTGTGAGAGCCCAGATTCTTGCAGAACCAGAATGGGTCATGCA

GCCATCCATAGATATCCAGCTGAACCGACGTCAGAGTACGGCCAAGAAGG

TGCTACTTTCCTACTACACCCAGTATTCTCAAACAATATCCACCAAAGATA

CAAGGCAGGCTCGGGGCAGAAGCTGGGTAATGATTGAGCCTAGTGGAGCT

GTGCAAAGAGATCAGACTGTCCTGGAAGGGGGTCCATGCCAGTTGAGCTG

CAACGTGAAAGCTTCTGAGAGTCCATCTATCTTCTGGGTGCTTCCAGATGG

CTCCATCCTGAAAGCGCCCATGGATGACCCAGACAGCAAGTTCTCCATTCT

CAGCAGTGGCTGGCTGAGGATCAAGTCCATGGAGCCATCTGACTCAGGCT

TGTACCAGTGCATTGCTCAAGTGAGGGATGAAATGGACCGCATGGTATAT

AGGGTACTTGTGCAGTCTCCCTCCACTCAGCCAGCCGAGAAAGACACAGT

GACAATTGGCAAGAACCCAGGGGAGTCGGTGACATTGCCTTGCAATGCTT

TAGCAATACCCGAAGCCCACCTTAGCTGGATTCTTCCAAACAGAAGGATA

ATTAATGATTTGGCTAACACATCACATGTATACATGTTGCCAAATGGAACT

CTTTCCATCCCAAAGGTCCAAGTCAGTGATAGTGGTTACTACAGATGTGTG

GCTGfCAACCAGCAAGGGGCAGACCATTTTACGGTGGGAATCACAGTGAC

CAAGAAAGGGTCTGGCTTGCCATCCAAAAGAGGCAGACGCCCAGGTGCA

AAGGCTCTTTCCAGAGTCAGAGAAGACATCGTGGAGGATGAAGGGGGCTC

GGGCATGGGAGATGAAGAGAACACTTCAAGGAGACTTCTGCATCCAAAG

GACCAAGAGGTGTTCCTCAAAACAAAGGATGATGCCATCAATGGAGACA

AGAAAGCCAAGAAAGGGAGAAGAAAGCTGAAACTCTGGAAGCATTCGGA

AAAAGAACCAGAGACCAATGTTGCAGAAGGTCGCAGAGTGTTTGAATCTA

GACGAAGGATAAACATGGCAAACAAACAGATTAATCCGGAGCGCTGGGC

TGATATTTTAGCCAAAGTCCGTGGGAAAAATCTCCCTAAGGGCACAGAAG

TACCCCCGTTGATTAAAACCACAAGTCCTCCATCCTTGAGCCTAGAAGTCA

CACCACCTTTTCCTGCTGTTTCTCCCCCCTCAGCATCTCCTGTGCAGACAGT

AACCAGTGCTGAAGAATCCTCAGCAGATGTACCTCTACTTGGTGAAGAAG

AGCACGTTTTGGGTACCATTTCCTCAGCCAGCATGGGGCTAGAACACAAC

CACAATGGAGTTATTCTTGTTGAACCTGAAGTAACAAGCACACCTCTGGA

GGAAGTTGTTGATGACCTTTCTGAGAAGACTGAGGAGATAACTTCCACTG

AAGGAGACCTGAAGGGGACAGCAGCCCCTACACTTATATCTGAGCCTTAT

GAACCATCTCCTACTCTGCACACATTAGACACAGTCTATGAAAAGCCCAC

CCATGAAGAGACGGCAACAGAGGGTTGGTCTGCAGCAGATGTTGGATCGT

CACCAGAGCCCACATCCAGTGAGTATGAGCCTCCATTGGATGCTGTCTCCT

TGGCTGAGTCTGAGCCCATGCAATACTTTGACCCAGATTTGGAGACTAAG

TCACAACCAGATGAGGATAAGATGAAAGAAGACACCTTTGCACACCTTAC

TCCAACCCCCACCATCTGGGTTAATGACTCCAGTACATCACAGTTATTTGA

GGATTCTACTATAGGGGAACCAGGTGTCCCAGGCCAATCACATCTACAAG 95238.doc -64- 1327645

GACTGACAGACAACATCCACCTTGTGAAAAGTAGTCTAAGCACTCAAGAC

ACCTTACTGATTAAAAAGGGTATGAAAGAGATGTCTCAGACACTACAGGG

AGGAAATATGCTAGAGGGAGACCCCACACACTCCAGAAGTTCTGAGAGTG

AGGGCCAAGAGAGCAAATCCATCACTTTGCCTGACTCCACACTGGGTATA

ATGAGCAGTATGTCTCCAGTTAAGAAGCCTGCGGAAACCACAGTTGGTAC

CCTCCTAGACAAAGACACCACAACAGTAACAACAACACCAAGGCAAAAA

GTTGCTCCGTCATCCACCATGAGCACTCACCCTTCTCGAAGGAGACCCAAC

GGGAGAAGGAGATTACGCCCCAACAAATTCCGCCACCGGCACAAGCAAA

CCCCACCCACAACTTTTGCCCCATCAGAGACTTTTTCTACTCAACCAACTC

AAGCACCTGACATTAAGATTTCAAGTCAAGTGGAGAGTTCTCTGGTTCCTA

CAGCTTGGGTGGATAACACAGTTAATACCCCCAAACAGTTGGAAATGGAG

AAGAATGCAGAACCCACATCCAAGGGAACACCACGGAGAAAACACGGGA

AGAGGCCAAACAAACATCGATATACCCCTTCTACAGTGAGCTCAAGAGCG

TCCGGATCCAAGCCCAGCCCTTCTCCAGAAAATAAACATAGAAACATTGT

TACTCCCAGTTCAGAAACTATACTTTTGCCTAGAACTGTTTCTCTGAAAAC

TGAGGGCCCTTATGATTCCTTAGATTACATGACAACCACCAGAAAAATAT

ATTCATCTTACCCTAAAGTCCAAGAGACACTTCCAGTCACATATAAACCCA

CATCAGATGGAAAAGAAATTAAGGATGATGTTGCCACAAATGTTGACAAA

CATAAAAGTGACATTTTAGTCACTGGTGAATCAATTACTAATGCCATACCA

ACTTCTCGCTCCTTGGTCTCCACTATGGGAGAATTTAAGGAAGAATCCTCT

CCTGTAGGCTTTCCAGGAACTCCAACCTGGAATCCCTCAAGGACGGCCCA

GCCTGGGAGGCTACAGACAGACATACCTGTTACCACTTCTGGGGAAAATC

TTACAGACCCTCCCCTTCTTAAAGAGCTTGAGGATGTGGATTTCACTTCCG

AGTTTTTGTCCTCTTTGACAGTCTCCACACCATTTCACCAGGAAGAAGCTG

GTTCTTCCACAACTCTCTCAAGCATAAAAGTGGAGGTGGCTTCAAGTCAG

GCAGAAACCACCACCCTTGATCAAGATCATCTTGAAACCACTGTGGCTAT

TCTCCTTTCTGAAACTAGACCACAGAATCACACCCCTACTGCTGCCCGGAT

GAAGGAGCCAGCATCCTCGTCCCCATCCACAATTCTCATGTCTTTGGGACA

AACCACCACCACTAAGCCAGCACTTCCCAGTCCAAGAATATCTCAAGCAT

CTAGAGATTCCAAGGAAAATGTTTTCTTGAATTATGTGGGGAATCCAGAA

ACAGAAGCAACCCCAGTCAACAATGAAGGAACACAGCATATGTCAGGGC

CAAATGAATTATCAACACCCTCTTCCGACCGGGATGCATTTAACTTGTCTA

CAAAGCTGGAATTGGAAAAGCAAGTATTTGGTAGTAGGAGTCTACCACGT

GGCCCAGATAGCCAACGCCAGGATGGAAGAGTTCATGCTTCTCATCAACT

AACCAGAGTCCCTGCCAAACCCATCCTACCAACAGCAACAGTGAGGCTAC

CTGAAATGTCCACACAAAGCGCTTCCAGATACTTTGTAACTTCCCAGTCAC

CTCGTCACTGGACCAACAAACCGGAAATAACTACATATCCTTCTGGGGCT

TTGCCAGAGAACAAACAGTTTACAACTCCAAGATTATCAAGTACAACAAT

TCCTCTCCCATTGCACATGTCCAAACCCAGCATTCCTAGTAAGTTTACTGA

CCGAAGAACTGACCAATTCAATGGTTACTCCAAAGTGTTTGGAAATAACA

ACATCCCTGAGGCAAGAAACCCAGTTGGAAAGCCTCCCAGTCCAAGAATT

CCTCATTATTCCAATGGAAGACTCCCTTTCTTTACCAACAAGACTCTTTCTT

TTCCACAGTTGGGAGTCACCCGGAGACCCCAGATACCCACTTCTCCTGCCC 95238.doc -65- 1327645

CAGTAATGAGAGAGAGAAAAGTTATTCCAGGTTCCTACAACAGGATACAT

TCCCATAGCACCTTCCATCTGGACTTTGGCCCTCCGGCACCTCCGTTGTTG

CACACTCCGCAGACCACGGGATCACCCTCAACTAACTTACAGAATATCCC

TATGGTCTCTTCCACCCAGAGTTCTATCTCCTTTATAACATCTTCTGTCCAG

TCCTCAGGAAGCTTCCACCAGAGCAGCTCAAAGTTCTTTGCAGGAGGACC

TCCTGCATCCAAATTCTGGTCTCTTGGGGAAAAGCCCCAAATCCTCACCAA

GTCCCCACAGACTGTGTCCGTCACCGCTGAGACAGACACTGTGTTCCCCTG

TGAGGCAACAGGAAAACCAAAGCCTTTCGTTACTTGGACAAAGGTTTCCA

CAGGAGCTCTTATGACTCCGAATACCAGGATACAACGGTTTGAGGTTCTC

AAGAACGGTACCTTAGTGATACGGAAGGTTCAAGTACAAGATCGAGGCCA

GTATATGTGCACCGCCAGCAACCTGCACGGCCTGGACAGGATGGTGGTCT

TGCTTTCGGTCACCGTGCAGCAACCTCAAATCCTAGCCTCCCACTACCAGG

ACGTCACTGTCTACCTGGGAGACACCATTGCAATGGAGTGTCTGGCCAAA

GGGACCCCAGCCCCCCAAATTTCCTGGATCTTCCCTGACAGGAGGGTGTG

GCAAACTGTGTCCCCCGTGGAGAGCCGCATCACCCTGCACGAAAACCGGA

CCCTTTCCATCAAGGAGGCGTCCTTCTCAGACAGAGGCGTCTATAAGTGC

GTGGCCAGCAATGCAGCCGGGGCGGACAGCCTGGCCATCCGCCTGCACGT

GGCGGCACTGCCCCCCGTTATCCACCAGGAGAAGCTGGAGAACATCTCGC

TGCCCCCGGGGCTCAGCATTCACATTCACTGCACTGCCAAGGCTGCGCCCC

TGCCCAGCGTGCGCTGGGTGCTCGGGGACGGTACCCAGATCCGCCCCTC

CAGTTCCTCCACGGGAACTTGTTTGTTTTCCCCAACGGGACGCTCTACATC

CGCAACCTCGCGCCCAAGGACAGCGGGCGCTATGAGTGCGTGGCCGCCAA

CCTGGTAGGCTCCGCGCGCAGGACGGTGCAGCTGAACGTGCAGCGTGCAG

CAGCCAACGCGCGCATCACGGGCACCTCCCCGCGGAGGACGGACGTCAG

GTACGGAGGAACCCTCAAGCTGGACTGCAGCGCCTCGGGGGACCCCTGGC

CGCGCATCCTCTGGAGGCTGCCGTCCAAGAGGATGATCGACGCGCTCTTC

AGTTTTGATAGCAGAATCAAGGTGTTTGCCAATGGGACCCTGGTGGTGAA

ATCAGTGACGGACAAAGATGCCGGAGATTACCTGTGCGTAGCTCGAAATA

AGGTTGGTGATGACTACGTGGTGCTCAAAGTGGATGTGGTGATGAAACCG

GCCAAGATTGAACACAAGGAGGAGAACGACCACAAAGTCTTCTACGGGG

GTGACCTGAAAGTGGACTGTGTGGCCACCGGGCTTCCCAATCCCGAGATC

TCCTGGAGCCTCCCAGACGGGAGTCTGGTGAACTCCTTCATGCAGTCGGA

TGACAGCGGTGGACGCACCAAGCGCTATGTCGTCTTCAACAATGGGACAC

TCTACTTTAACGAAGTGGGGATGAGGGAGGAAGGAGACTACACCTGCTTT

GCTGAAAATCAGGTCGGGAAGGACGAGATGAGAGTCAGAGTCAAGGTGG

TGACAGCGCCCGCCACCATCCGGAACAAGACTTACTTGGCGGTTCAGGTG

CCCTATGGAGACGTGGTCACTGTAGCCTGTGAGGCCAAAGGAGAACCCAT

GCCCAAGGTGACTTGGTTGTCCCCAACCAACAAGGTGATCCCCACCTCCTC

TGAGAAGTATCAGATATACCAAGATGGCACTCTCCTTATTCAGAAAGCCC

AGCGTTCTGACAGCGGCAACTACACCTGCCTGGTCAGGAACAGCGCGGGA

GAGGATAGGAAGACGGTGTGGATTCACGTCAACGTCCAGCCACCCAAGAT

CAACGGTAACCCCAACCCCATCACCACCGTGCGGGAGATAGCAGCCGGGG

GCAGTCGGAAACTGATTGACTGCAAAGCTGAAGGCATCCCCACCCCGAGG 95238.doc -66- 1327645

GTGTTATGGGCTTTTCCCGAGGGTGTGGTTCTGCCAGCTCCATACTATGGA

AACCGGATCACTGTCCATGGCAACGGTTCCCTGGACATCAGGAGTTTGAG

GAAGAGCGACTCCGTCCAGCTGGTATGCATGGCACGCAACGAGGGAGGG

GAGGCGAGGTTGATCGTGCAGCTCACTGTCCTGGAGCCCATGGAGAAACC

CATCTTCCACGACCCGATCAGCGAGAAGATCACGGCCATGGCGGGCCACA

CCATCAGCCTCAACTGCTCTGCCGCGGGGACCCCGACACCCAGCCTGGTG

TGGGTCCTTCCCAATGGCACCGATCTGCAGAGTGGACAGCAGCTGCAGCG

CTTCTACCACAAGGCTGACGGCATGCTACACATTAGCGGTCTCTCCTCGGT

GGACGCTGGGGCCTACCGCTGCGTGGCCCGCAATGCCGCTGGCCACACGG

AGAGGCTGGTCTCCCTGAAGGTGGGACTGAAGCCAGAAGCAAACAAGCA

GTATCATAACCTGGTCAGCATCATCAATGGTGAGACCCTGAAGCTCCCCT

GCACCCCTCCCGGGGCTGGGCAGGGACGTTTCTCCTGGACGCTCCCCAAT

GGCATGCATCTGGAGGGCCCCCAAACCCTGGGACGCGTTTCTCTTCTGGA

CAATGGCACCCTCACGGTTCGTGAGGCCTCGGTGTTTGACAGGGGTACCT

ATGTATGCAGGATGGAGACGGAGTACGGCCCTTCGGTCACCAGCATCCCC

GTGATTGTGATCGCCTATCCTCCCCGGATCACCAGCGAGCCCACCCCGGTC

ATCTACACCCGGCCCGGGAACACCGTGAAACTGAACTGCATGGCTATGGG

GATTCCCAAAGCTGACATCACGTGGGAGTTACCGGATAAGTCGCATCTGA

AGGCAGGGGTTCAGGCTCGTCTGTATGGAAACAGATTTCTTCACCCCCAG

GGATCACTGACCATCCAGCATGCCACACAGAGAGATGCCGGCTTCTACAA

GTGCATGGCAAAAAACATTCTCGGCAGTGACTCCAAAACAACTTACATCC

ACGTCTTCTGAAATGTGGATTCCAGAATGATTGCTTAGGAACTGACAACA

AAGCGGGGTTTGTAAGGGAAGCCAGGTTGGGGAATAGGAGCTCTTAAATA

ATGTGTCACAGTGCATGGTGGCCTCTGGTGGGTTTCAAGTTGAGGTTGATC

TTGATCTACAATTGTTGGGAAAAGGAAGCAATGCAGACACGAGAAGGAG

GGCTCAGCCTTGCTGAGACACTTTCTTTTGTGTTTACATCATGCCAGGGGC

TTCATTCAGGGTGTCTGTGCTCTGACTGCAATTTTTCTTCTTTTGCAAATGC

CACTCGACTGCCTTCATAAGCGTCCATAGGATATCTGAGGAACATTCATCA

AAAATAAGCCATAGACATGAACAACACCTCACTACCCCATTGAAGACGCA

TCACCTAGTTAACCTGCTGCAGTTTTTACATGATAGACTTTGTTCCAGATT

GACAAGTCATCTTTCAGTTATTTCCTCTGTCACTTCAAAACTCCAGCTTGC

CCAATAAGGATTTAGAACCAGAGTGACTGATATATATATATATATTTTAAT

TCAGAGTTACATACATACAGCTACCATTTTATATGAAAAAAGAAAAACAT

TTCTTCCTGGAACTCACTTTTTATATAATGTTTTATATATATATTTTTTCCTT

TCAAATCAGACGATGAGACTAGAAGGAGAAATACTTTCTGTCTTATTAAA

ATTAATAAATTATTGGTCTTTACAAGACTTGGATACATTACAGCAGACATG

GAAATATAATTTTAAAAAATTTCTCTCCAACCTCCTTCAAATTCAGTCACC

ACTGTTATATTACCTTCTCCAGGAACCCTCCAGTGGGGAAGGCTGCGATAT

TAGATTTCCTTGTATGCAAAGTTTTTGTTGAAAGCTGTGCTCAGAGGAGGT

GAGAGGAGAGGAAGGAGAAAACTGCATCATAACTTTACAGAATTGAATC

TAGAGTCTTCCCCGAAAAGCCCAGAAACTTCTCTGCAGTATCTGGCTTGTC

CATCTGGTCTAAGGTGGCTGCTTCTTCCCCAGCCATGAGTCAGTTTGTGCC

CATGAATAATACACGACCTGTTATTTCCATGACTGCTTTACTGTATTTTTA 95238.doc -67- 1327645 AGGTCAATATACTGTACATTTGATAATAAAATAATATTCTCCCAAAAAAA AAA Sequence No. 95

Cysteamine chymotrypsin inhibitor SA >gi丨19882252 | ref丨NM_001322.2 | Human cysteine protease inhibitor SA (CST2), mRNA | qPCR advance _ primer matches [302..320]|9卩匚11 reverse „引子 matches [393..370]|9? ft ruler matches [341..369]

GCCTCCGAGGAGACCATGGCCTGGCCCCTGTGCACCCTGCTGCTCC

TGCTGGCCACCCAGGCTGTGGCCCTGGCCTGGAGCCCCCAGGAGGAGGAC

AGGATAATCGAGGGTGGCATCTATGATGCAGACCTCAATGATGAGCGGGT

ACAGCGTGCCCTTCACTTTGTCATCAGCGAGTATAACAAGGCCACTGAAG

ATGAGTACTACAGACGCCTGCTGCGGGTGCTACGAGCCAGGGAGCAGATC

GTGGGCGGGGTGAATTACTTCTTCGACATAGAGGTGGGCCGAACCATATG

TACCAAGTCCCAGCCCAACTTGGACACCTGTGCCTTCCATGAACAGCCAG

AACTGCAGAAGAAACAGTTGTGCTCTTTCCAGATCTACGAAGTTCCCTGG

GAGGACAGAATGTCCCTGGTGAATTCCAGGTGTCAAGAAGCCTAGGGATC

TGTGCCAGGGAGTCACACTGACCACCTCCTACTCCCACCCCTTGTAGTGCT

CCCACCCCTGGACTGGTGGCCCCCACCCTGTGGGAGGTCTCCCCATGCACC

TGCAGCAGGAGAAGACAGAGAAGGCTGCAGGAGGCCTTTGTTGCTCAGC

AGGGGACTCTGCCCTCCCTCCTTCCTTTTGCTTCTCATAGCCCTGGTACATG

GTACACACACCCCCACCTCCTGCAATTAAACAGTAGCATCACCTC Sequence No. 96

Cysteamine chymotrypsin inhibitor SN >gi 丨1988225 0 | ref | ΝΜ_001898.2 | Human cysteine protease inhibitor SN (CST1), mRNA | qPCR advance _ primer and [358..376] | qPCH reverse-bow|子与[449..426]相相| qPCR probe matches [397·.425]

GGGCTCCCTGCCTCGGGCTCTCACCCTCCTCTCCTGCAGCTCCAGCT

TTGTGCTCTGCCTCTGAGGAGACCATGGCCCAGTATCTGAGTACCCTGCTG

CTCCTGCTGGCCACCCTAGCTGTGGCCCTGGCCTGGAGCCCCAAGGAGGA

GGATAGGATAATCCCGGGTGGCATCTATAACGCAGACCTCAATGATGAGT

GGGTACAGCGTGCCCTTCACTTCGCCATCAGCGAGTATAACAAGGCCACC

AAAGATGACTACTACAGACGTCCGCTGCGGGTACTAAGAGCCAGGCAACA 95238.doc • 68 · 1327645 GACCGTTGGGGGGGTGAATTACTTCTTCGACGTAGAGGTGGGCCGCACCA TATGTACCAAGTCCCAGCCCAACTTGGACACCTGTGCCTTCCATGAACAGC CAGAACTGCAGAAGAAACAGTTGTGCTCTTTCGAGATCTACGAAGTTCCC TGGGAGAACAGAAGGTCCCTGGTGAAATCCAGGTGTCAAGAATCCTAGGG ATCTGTGCCAGGCCATTCGCACCAGCCACCACCCACTCCCACCCCCTGTAG TGCTCCCACCCCTGGACTGGTGGCCCCCACCCTGCGGGAGGCCTCCCCATG TGCCTGCGCCAAGAGACAGACAGAGAAGGCTGCAGGAGTCCTTTGTTGCT CAGCAGGGCGCTCTGCCCTCCCTCCTTCCTTCTTGCTTCTAATAGCCCTGGT ACATGGTACACACCCCCCCACCTCCTGCAATTAAACAGTAGCATCGCCTCC CTCTGAAAAAAAAAAAAAAAAAAAAAAA Sequence No. 97 from the bad acyl amine oxidase - Enzyme Class 2

Gt | 460 | | | | | | | | | 2269] Matching I qPCR probes match [2261..2229]

ACTCCAGCGCGCGGCTACCTACGCTTGGTGCTTGCTTTCTCCAGCCA

TCGGAGACCAGAGCCGCCCCCTCTGCTCGAGAAAGGGGCTCAGCGGCGGC

GGAAGCGGAGGGGGACCACCGTGGAGAGCGCGGTCCCAGCCCGGCCACT

GCGGATCCCTGAAACCAAAAAGCTCCTGCTGCTTCTGTACCCCGCCTGTCC

CTCCCAGCTGCGCAGGGCCCCTTCGTGGGATCATCAGCCCGAAGACAGGG

ATGGAGAGGCCTCTGTGCTCCCACCTCTGCAGCTGCCTGGCTATGCTGGCC

CTCCTGTCCCCCCTGAGCCTGGCACAGTATGACAGCTGGCCCCATTACCCC

GAGTACTTCCAGCAACCGGCTCCTGAGTATCACCAGCCCCAGGCCCCCGC

CAACGTGGCCAAGATTCAGCTGCGCCTGGCTGGGCAGAAGAGGAAGCAC

AGCGAGGGCCGGGTGGAGGTGTACTATGATGGCCAGTGGGGCACCGTGTG

CGATGACGACTTCTCCATCCACGCTGCCCACGTCGTCTGCCGGGAGCTGG

GCTATGTGGAGGCCAAGTCCTGGACTGCCAGCTCCTCCTACGGCAAGGGA

GAAGGGCCCATCTGGTTAGACAATCTCCACTGTACTGGCAACGAGGCGAC

CCTTGCAGCATGCACCTCCAATGGCTGGGGCGTCACTGACTGCAAGCACA

CGGAGGATGTCGGTGTGGTGTGCAGCGACAAAAGGATTCCTGGGTTCAAA

TTTGACAATTCGTTGATCAACCAGATAGAGAACCTGAATATCCAGGTGGA

GGACATTCGGATTCGAGCCATCCTCTCAACCTACCGCAAGCGCACCCCAG

TGATGGAGGGCTACGTGGAGGTGAAGGAGGGCAAGACCTGGAAGCAGAT

CTGTGACAAGCACTGGACGGCCAAGAATTCCCGCGTGGTCTGCGGCATGT

TTGGCTTCCCTGGGGAGAGGACATACAATACCAAAGTGTACAAAATGTTT

GCCTCACGGAGGAAGCAGCGCTACTGGCCATTCTCCATGGACTGCACCGG

CACAGAGGCCCACATCTCCAGCTGCAAGCTGGGCCCCCAGGTGTCACTGG

ACCCCATGAAGAATGTCACCTGCGAGAATGGGCTGCCGGCCGTGGTGAGT 95238.doc •69- 1327645

TGTGTGCCTGGGCAGGTCTTCAGCCCTGACGGACCCTCGAGATTCCGGAA

AGCATACAAGCCAGAGCAACCCCTGGTGCGACTGAGAGGCGGTGCCTACA

TCGGGGAGGGCCGCGTGGAGGTGCTCAAAAATGGAGAATGGGGGACCGT

CTGCGACGACAAGTGGGACCTGGTGTCGGCCAGTGTGGTCTGCAGAGAGC

TGGGCTTTGGGAGTGCCAAAGAGGCAGTCACTGGCTCCCGACTGGGGCAA

GGGATCGGACCCATCCACCTCAACGAGATCCAGTGCACAGGCAATGAGAA

GTCCATTATAGACTGCAAGTTCAATGCCGAGTCTCAGGGCTGCAACCACG

AGGAGGATGCTGGTGTGAGATGCAACACCCCTGCCATGGGCTTGCAGAAG

AAGCTGCGCCTGAACGGCGGCCGCAATCCCTACGAGGGCCGAGTGGAGGT

GCTGGTGGAGAGAAACGGGTCCCTTGTGTGGGGGATGGTGTGTGGCCAAA

ACTGGGGCATCGTGGAGGCCATGGTGGTCTGCCGCCAGCTGGGCCTGGGA

TTCGCCAGCAACGCCTTCCAGGAGACCTGGTATTGGCACGGAGATGTCAA

CAGCAACAAAGTGGTCATGAGTGGAGTGAAGTGCTCGGGAACGGAGCTG

TCCCTGGCGCACTGCCGCCACGACGGGGAGGACGTGGCCTGCCCCCAGGG

CGGAGTGCAGTACGGGGCCGGAGTTGCCTGCTCAGAAACCGCCCCTGACC

TGGTCCTCAATGCGGAGATGGTGCAGCAGACCACCTACCTGGAGGACCGG

CCCATGTTCATGCTGCAGTGTGCCATGGAGGAGAACTGCCTCTCGGCCTCA

GCCGCGCAGACCGACCCCACCACGGGCTACCGCCGGCTCCTGCGCTTCTC

CTCCCAGATCCACAACAATGGCCAGTCCGACTTCCGGCCCAAGAACGGCC

GCCACGCGTGGATCTGGCACGACTGTCACAGGCACTACCACAGCATGGAG

GTGTTCACCCACTATGACCTGCTGAACCTCAATGGCACCAAGGTGGCAGA

GGGCCACAAGGCCAGCTTCTGCTTGGAGGACACAGAATGTGAAGGAGAC

ATCCAGAAGAATTACGAGTGTGCCAACTTCGGCGATCAGGGCATCACCAT

GGGCTGCTGGGACATGTACCGCCATGACATCGACTGCCAGTGGGTTGACA

TCACTGACGTGCCCCCTGGAGACTACCTGTTCCAGGTTGTTATTAACCCCA

ACTTCGAGGTTGCAGAATCCGATTACTCCAACAACATCATGAAATGCAGG

AGCCGCTATGACGGCCACCGCATCTGGATGTACAACTGCCACATAGGTGG

TTCCTTCAGCGAAGAGACGGAAAAAAAGTTTGAGCACTTCAGCGGGCTCT

TAAACAACCAGCTGTCCCCGCAGTAAAGAAGCCTGCGTGGTCAACTCCTG

TCTTCAGGCCACACCACATCTTCCATGGGACTTCCCCCCAACAACTGAGTC

TGAACGAATGCCACGTGCCCTCACCCAGCCCGGCCCCCACCCTGTCCAGA

CCCCTACAGCTGTGTCTAAGCTCAGGAGGAAAGGGACCCTCCCATCATTC

ATGGGGGGCTGCTACCTGACCCTTGGGGCCTGAGAAGGCCTTGGGGGGGT

GGGGTTTGTCCACAGAGCTGCTGGAGCAGCACCAAGAGCCAGTCTTGACC

GGGATGAGGCCCACAGACAGGTTGTCATCAGCTTGTCCCATTCAAGCCAC

CGAGCTCACCACAGACACAGTGGAGCCGCGCTCTTCTCCAGTGACACGTG

GACAAATGCGGGCTCATCAGCCCCCCCAGAGAGGGTCAGGCCGAACCCCA

TTTCTCCTCCTCTTAGGTCATTTTCAGCAAACTTGAATATCTAGACCTCTCT

TCCAATGAAACCCTCCAGTCTATTATAGTCACATAGATAATGGTGCCACGT

GTTTTCTGATTTGGTGAGCTCAGACTTGGTGCTTCCCTCTCCACAACCCCC

ACCCCTTGTTTTTCAAGATACTATTATTATATTTTCACAGACTTTTGAAGCA

CAAATTTATTGGCATTTAATATTGGACATCTGGGCCCTTGGAAGTACAAAT

CTAAGGAAAAACCAACCCACTGTGTAAGTGACTCATCTTCCTGTTGTTCCA 95238.doc -70- 1327645

ATTCTGTGGGTTTTTGATTCAACGGTGCTATAACCAGGGTCCTGGGTGACA

GGGCGCTCACTGAGCACCATGTGTCATCACAGACACTTACACATACTTGA AACTTGGAATAAAAGAAAGATTTATG Sequence No. 98 thyroglobulin > gi丨33 5 89851 I ref I NM_003235.3 丨 Human thyroglobulin (TG), mRNA | qPCR advance _ primer matched with [886..905] 丨qPCR reverse-introduction Matching [962..941] I qPCR probe matches [915..939]

GCAGTGGTTTCTCCTCCTTCCTCCCAGGAAGGGCCAGGAAAATGGC

CCTGGTCCTGGAGATCTTCACCCTGCTGGCCTCCATCTGCTGGGTGTCGGC

CAATATCTTCGAGTACCAGGTTGATGCCCAGCCCCTTCGTCCCTGTGAGCT

GCAGAGGGAAACGGCCTTTCTGAAGCAAGCAGACTACGTGCCCCAGTGTG

CAGAGGATGGCAGCTTCCAGACTGTCCAGTGCCAGAACGACGGCCGCTCC

TGCTGGTGTGTGGGTGCCAACGGCAGTGAAGTGCTGGGCAGCAGGCAGCC

AGGACGGCCTGTGGCTTGTCTGTCATTTTGTCAGCTACAGAAACAGCAGA

TCTTACTGAGTGGCTACATTAACAGCACAGACACCTCCTACCTCCCTCAGT

GTCAGGATTCAGGGGACTACGCGCCTGTTCAGTGTGATGTGCAGCATGTC

CAGTGCTGGTGTGTGGACGCAGAGGGGATGGAGGTGTATGGGACCCGCCA

GCTGGGGAGGCCAAAGCGATGTCCAAGGAGCTGTGAAATAAGAAATCGT

CGTCTTCTCCACGGGGTGGGAGATAAGTCACCACCCCAGTGTTCTGCGGA

GGGAGAGTTTATGCCTGTCCAGTGCAAATTTGTCAACACCACAGACATGA

TGATTTTTGATCTGGTCCACAGCTACAACAGGTTTCCAGATGCATTTGTGA

CCTTCAGTTCCTTCCAGAGGAGGTTCCCTGAGGTATCTGGGTATTGCCACT

GTGCTGACAGCCAAGGGCGGGAACTGGCTGAGACAGGTTTGGAGTTGTTA

CTGGATGAAATTTATGACACCATTTTTGCTGGCCTGGACCTTCCTTCCACC

TTCACTGAAACCACCCTGTACCGGATACTGCAGAGACGGTTCCTCGCAGTT

CAATCAGTCATCTCTGGCAGATTCCGATGCCCCACAAAATGTGAAGTGGA

GCGGTTTACAGCAACCAGCTTTGGTCACCCCTATGTTCCAAGCTGCCGCCG

AAATGGCGACTATCAGGCGGTGCAGTGCCAGACGGAAGGGCCCTGCTGGT

GTGTGGACGCCCAGGGGAAGGAAATGCATGGAACCCGGCAGCAAGGGGA

GCCGCCATCTTGTGCTGAAGGCCAATCTTGTGCCTCCGAAAGGCAGCAGG

CCTTGTCCAGACTCTACTTTGGGACCTCAGGCTACTTCAGCCAGCACGACC

TGTTCTCTTCCCCAGAGAAAAGATGGGCCTCTCCAAGAGTAGCCAGATTT

GCCACATCCTGCCCACCCACGATCAAGGAGCTCTTTGTGGACTCTGGGCTT

CTCCGCCCAATGGTGGAGGGACAGAGCCAACAGTTTTCTGTCTCAGAAAA

TCTTCTCAAAGAAGCCATCCGAGCAATTTTTCCCTCCCGAGGGCTGGCTCG

TCTTGCCCTTCAGTTTACCACCAACCCAAAGAGACTCCAGCAAAACCTTTT

TGGAGGGAAATTTTTGGTGAATGTTGGCCAGTTTAACTTGTCTGGAGCCCT

TGGCACAAGAGGCACATTTAACTTCAGTCAATTTTTCCAGCAACTTGGTCT

TGCAAGCTTCTTGAATGGAGGGAGACAAGAAGATTTGGCCAAGCCACTCT 95238.doc 71 1327645

CTGTGGGATTAGATTCAAATTCTTCCACAGGAACCCCTGAAGCTGCTAAG

AAGGATGGTACTATGAATAAGCCAACTGTGGGCAGCTTTGGCTTTGAAAT

TAACCTACAAGAGAACCAAAATGCCCTCAAATTCCTTGCTTCTCTCCTGGA

GCTTCCAGAATTCCTTCTCTTCTTGCAACATGCTATCTCTGTGCCAGAAGA

TGTGGCAAGAGATTTAGGTGATGTGATGGAAACGGTACTCGACTCCCAGA

CCTGTGAGCAGACACCTGAAAGGCTATTTGTCCCATCATGCACGACAGAA

GGAAGCTATGAGGATGTCCAATGCTTTTCCGGAGAGTGCTGGTGTGTGAA

TTCCTGGGGCAAAGAGCTTCCAGGCTCAAGAGTCAGAGATGGACAGCCAA

GGTGCCCCACAGACTGTGAAAAGCAAAGGGCTCGCATGCAAAGCCTCATG

GGCAGCCAGCCTGCTGGCTCCACCTTGTTTGTCCCTGCTTGTACTAGTGAG

GGACATTTCCTGCCTGTCCAGTGCTTCAACTCAGAGTGCTACTGTGTTGAT

GCTGAGGGTCAGGCCATTCCTGGAACTCGAAGTGCAATAGGGAAGCCCAA

GAAATGCCCCACGCCCTGTCAATTACAGTCTGAGCAAGCTTTCCTCAGGA

CGGTGCAGGCCCTGCTCTCTAACTCCAGCATGCTACCCACCCTTTCCGACA

CCTACATCCCACAGTGCAGCACCGATGGGCAGTGGAGACAAGTGCAATGC

AATGGGCCTCCTGAGCAGGTCTTCGAGTTGTACCAACGATGGGAGGCTCA

GAACAAGGGCCAGGATCTGACGCCTGCCAAGCTGCTAGTGAAGATCATGA

GCTACAGAGAAGCAGCTTCCGGAAACTTCAGTCTCTTTATTCAAAGTCTGT

ATGAGGCTGGCCAGCAAGATGTCTTCCCGGTGCTGTCACAATACCCTTCTC

TGCAAGATGTCCCACTAGCAGCACTGGAAGGGAAACGGCCCCAGCCCAG

GGAGAATATCCTCCTGGAGCCCTACCTCTTCTGGCAGATCTTAAATGGCCA

ACTCAGCCAATACCCGGGGTCCTACTCAGACTTCAGCACTCCTTTGGCACA

TTTTGATCTTCGGAACTGCTGGTGTGTGGATGAGGCTGGCCAAGAACTGG

AAGGAATGCGGTCTGAGCCAAGCAAGCTCCCAACGTGTCCTGGCTCCTGT

GAGGAAGCAAAGCTCCGTGTACTGCAGTTCATTAGGGAAACGGAAGAGA

TTGTTTCAGCTTCCAACAGTTCTCGGTTCCCTCTGGGGGAGAGTTTCCTGG

TGGCCAAGGGAATCCGGCTGAGGAATGAGGACCTCGGCCTTCCTCCGCTC

TTCCCGCCCCGGGAGGCTTTCGCGGAGTTTCTGCGTGGGAGTGATTACGCC

ATTCGCCTGGCGGCTCAGTCTACCTTAAGCTTCTATCAGAGACGCCGCTTT

TCCCCGGACGACTCGGCTGGAGCATCCGCCCTTCTGCGGTCGGGCCCCTAC

ATGCCACAGTGTGATGCGTTTGGAAGTTGGGAGCCTGTGCAGTGCCACGC

TGGGACTGGGCACTGCTGGTGTGTAGATGAGAAAGGAGGGTTCATCCCTG

GCTCACTGACTGCCCGCTCTCTGCAGATTCCACAGTGCCCGACAACCTGCG

AGAAATCTCGAACCAGTGGGCTGCTTTCCAGTTGGAAACAGGCTAGATCC

CAAGAAAACCCATCTCCAAAAGACCTGTTCGTCCCAGCCTGCCTAGAAAC

AGGAGAATATGCCAGGCTGCAGGCATCGGGGGCTGGCACCTGGTGTGTGG

ACCCTGCATCAGGAGAAGAGTTGCGGCCTGGCTCGAGCAGCAGTGCCCAG

TGCCCAAGCCTCTGCAATGTGCTCAAGAGTGGAGTCCTCTCTAGGAGAGT

CAGCCCAGGCTATGTCCCAGCCTGCAGGGCAGAGGATGGGGGCTTTTCCC

CAGTGCAATGTGACCAGGCCCAGGGCAGCTGCTGGTGTGTCATGGACAGC

GGAGAAGAGGTGCCTGGGACGCGCGTGACCGGGGGCCAGCCCGCCTGTG

AGAGCCCGCGGTGTCCGCTGCCATTCAACGCGTCGGAGGTGGTTGGTGGA

ACAATCCTGTGTGAGACAATCTCGGGCCCCACAGGCTCTGCCATGCAGCA 95238.doc -72- 1327645

GTGCCAATTGCTGTGCCGCCAAGGCTCCTGGAGCGTGTTTCCACCAGGGC

CATTGATATGTAGCCTGGAGAGCGGACGCTGGGAGTCACAGCTGCCTCAG

CCCCGGGCCTGCCAACGGCCCCAGCTGTGGCAGACCATCCAGACCCAAGG

GCACTTTCAGCTCCAGCTCCCGCCGGGCAAGATGTGCAGTGCTGACTACG

CGGGTTTGCTGCAGACTTTCCAGGTTTTCATATTGGATGAGCTGACAGCCC

GCGGCTTCTGCCAGATCCAGGTGAAGACTTTTGGCACCCTGGTTTCCATTC

CTGTCTGCAACAACTCCTCTGTGCAGGTGGGTTGTCTGACCAGGGAGCGTT

TAGGAGTGAATGTTACATGGAAATCACGGCTTGAGGACATCCCAGTGGCT

TCTCTTCCTGACTTACATGACATTGAGAGAGCCTTGGTGGGCAAGGATCTC

CTTGGGCGCTTCACAGATCTGATCCAGAGTGGCTCATTCCAGCTTCATCTG

GACTCCAAGACGTTCCCAGCGGAAACCATCCGCTTCCTCCAAGGGGACCA

CTTTGGCACCTCTCCTAGGACACGGTTTGGGTGCTCGGAAGGATTCTACCA

AGTCTTGACAAGTGAGGCCAGTCAGGACGGACTGGGATGCGTTAAGTGCC

ATGAAGGAAGCTATTCCCAAGATGAGGAATGCATTCCTTGTCCTGTTGGA

TTCTACCAAGAACAGGCAGGGAGCTTGGCCTGTGTCCCATGTCCTGTGGG

CAGAACGACCATTTCTGCCGGAGCTTTCAGCCAGACTCACTGTGTCACTGA

CTGTCAGAGGAACGAAGCAGGCCTGCAATGTGACCAGAATGGCCAGTATC

GAGCCAGCCAGAAGGACAGGGGCAGTGGGAAGGCCTTCTGTGTGGACGG

CGAGGGGCGGAGGCTGCCATGGTGGGAAACAGAGGCCCCTCTTGAGGAC

TCACAGTGTTTGATGATGCAGAAGTTTGAGAAGGTTCCAGAATCAAAGGT

GATCTTCGACGCCAATGCTCCTGTGGCTGTCAGATCCAAAGTTCCTGATTC

TGAGTTCCCCGTGATGCAGTGCTTGACAGATTGCACAGAGGACGAGGCCT

GCAGCTTCTTCACCGTGTCCACGACGGAGCCAGAGATTTCCTGTGATTTCT

ATGCTTGGACAAGTGACAATGTTGCCTGCATGACTTCTGACCAGAAACGA

GATGCACTGGGGAACTCAAAGGCCACCAGCTTTGGAAGTCTTCGCTGCCA

GGTGAAAGTGAGGAGCCATGGTCAAGATTCTCCAGCTGTGTATTTGAAAA

AGGGCCAAGGATCCACCACAACACTTCAGAAACGCTTTGAACCCACTGGT

TTCCAAAACATGCTTTCTGGATTGTACAACCCCATTGTGTTCTCAGCCTCA

GGAGCCAATCTAACCGATGCTCACCTCTTCTGTCTTCTTGCATGCGACCGT

GATCTGTGTTGCGATGGCTTCGTCCTCACACAGGTTCAAGGAGGTGCCATC

ATCTGTGGGTTGCTGAGCTCACCCAGTGTCCTGCTTTGTAATGTCAAAGAC

TGGATGGATCCCTCTGAAGCCTGGGCTAATGCTACATGTCCTGGTGTGACA

TATGACCAGGAGAGCCACCAGGTGATATTGCGTCTTGGAGACCAGGAGTT

CATCAAGAGTCTGACACCCTTAGAAGGAACTCAAGACACCTTTACCAATT

TTCAGCAGGTTTATCTCTGGAAAGATTCTGACATGGGGTCTCGGCCTGAGT

CTATGGGATGTAGAAAAAACACAGTGCCAAGGCCAGCATCTCCAACAGA

AGCAGGTTTGACAACAGAACTTTTCTCCCCTGTGGACCTCAACCAGGTCAT

TGTCAATGGAAATCAATCACTATCCAGCCAGAAGCACTGGCTTTTCAAGC

ACCTGTTTTCAGCCCAGCAGGCAAACCTATGGTGCCTTTCTCGTTGTGTGC

AGGAGCACTCTTTCTGTCAGCTCGCAGAGATAACAGAGAGTGCATCCTTG

TACTTCACCTGCACCCTCTACCCAGAGGCACAGGTGTGTGATGACATCATG

GAGTCCAATACCCAGGGCTGCAGACTGATCCTGCCTCAGATGCCAAAGGC

CCTGTTCCGGAAGAAAGTTATACTGGAAGATAAAGTGAAGAACTTTTACA 95238.doc -73- 1327645

CTCGCCTGCCGTTCCAAAAACTGATGGGGATATCCATTAGAAATAAAGTG

CCCATGTCTGAAAAATCTATTTCTAATGGGTTCTTTGAATGTGAACGACGG

TGCGATGCGGACCCATGCTGCACTGGCTTTGGATTTCTAAATGTTTCCCAG

TTAAAAGGAGGAGAGGTGACATGTCTCACTCTGAACAGCTTGGGAATTCA

GATGTGCAGTGAGGAGAATGGAGGAGCCTGGCGCATTTTGGACTGTGGCT

CTCCTGACATTGAAGTCCACACCTATCCCTTCGGATGGTACCAGAAGCCCA

TTGCTCAAAATAATGCTCCCAGTTTTTGCCCTTTGGTTGTTCTGCCTTCCCT

CACAGAGAAAGTGTCTCTGGAATCGTGGCAGTCCCTGGCCCTCTCTTCAGT

GGTTGTTGATCCATCCATTAGGCACTTTGATGTTGCCCATGTCAGCACTGC

TGCCACCAGCAATTTCTCTGCTGTCCGAGACCTCTGTTTGTCGGAATGTTC

CCAACATGAGGCCTGTCTCATCACCACTCTGCAAACCCAACTCGGGGCTG

TGAGATGTATGTTCTATGCTGATACTCAAAGCTGCACACATAGTCTGCAGG

GTCGGAACTGCCGACTTCTGCTTCGTGAAGAGGCCACCCACATCTACCGG

AAGCCAGGAATCTCTCTGCTCAGCTATGAGGCATCTGTACCTTCTGTGCCC

ATTTCCACCCATGGCCGGCTGCTGGGCAGGTCCCAGGCCATCCAGGTGGG

TACCTCATGGAAGCAAGTGGACCAGTTCCTTGGAGTTCCATATGCTGCCCC

GCCCCTGGCAGAGAGGCACTTCCAGGCACCAGAGCCCTTGAACTGGACAG

GCTCCTGGGATGCCAGCAAGCCAAGGGCCAGCTGCTGGCAGCCAGGCACC

AGAACATCCACGTCTCCTGGAGTCAGTGAAGATTGTTTGTATCTCAATGTG

TTCATCCCTCAGAATGTGGCCCCTAACGCGTCTGTGCTGGTGTTCTTCCAC

AACACCATGGACAGGGAGGAGAGTGAAGGATGGCCGGCTATCGACGGCT

CCTTCTTGGCTGCTGTTGGCAACCTCATCGTGGTCACTGCCAGCTACCGAG

TGGGTGTCTTCGGCTTCCTGAGTTCTGGATCCGGAGAGGTGAGTGGCAACT

GGGGGCTGCTGGACCAGGTGGCGGCTCTGACCTGGGTGCAGACCCACATC

CGAGGATTTGGCGGGGACCCTCGGCGCGTGTCCCTGGCAGCAGACCGTGG

CGGGGCTGATGTGGCCAGCATCCACCTTCTCACGGCCAGGGCCACCAACT

CCCAACTTTTCCGGAGAGCTGTGCTGATGGGAGGCTCCGCACTCTCCCCGG

CCGCCGTCATCAGCCATGAGAGGGCTCAGCAGCAGGCAATTGCTTTGGCA

AAGGAGGTCAGTTGCCCCATGTCATCCAGCCAAGAAGTGGTGTCCTGCCT

CCGCCAGAAGCCTGCCAATGTCCTCAATGATGCCCAGACCAAGCTCCTGG

CCGTGAGTGGCCCTTTCCACTACTGGGGTCCTGTGATCGATGGCCACTTCC

TCCGTGAGCCTCCAGCCAGAGCACTGAAGAGGTCTTTATGGGTAGAGGTC

GATCTGCTCATTGGGAGTTCTCAGGACGACGGGCTCATCAACAGAGCAAA

GGCTGTGAAGCAATTTGAGGAAAGTCGAGGCCGGACCAGTAGCAAAACA

GCCTTTTACCAGGCACTGCAGAATTCTCTGGGTGGCGAGGACTCAGATGC

CCGCGTCGAGGCTGCTGCTACATGGTATTACTCTCTGGAGCACTCCACGGA

TGACTATGCCTCCTTCTCCCGGGCTCTGGAGAATGCCACCCGGGACTACTT

TATCATCTGCCCTATAATCGACATGGCCAGTGCCTGGGCAAAGAGGGCCC

GAGGAAACGTCTTCATGTACCATGCTCCTGAAAACTACGGCCATGGCAGC

CTGGAGCTGCTGGCGGATGTTCAGTTTGCCTTGGGGCTTCCCTTCTACCCA

GCCTACGAGGGGCAGTTTTCTCTGGAGGAGAAGAGCCTGTCGCTGAAAAT

CATGCAGTACTTTTCCCACTTCATCAGATCAGGAAATCCCAACTACCCTTA

TGAGTTCTCACGGAAAGTACCCACATTTGCAACCCCCTGGCCTGACTTTGT 95238.doc -74- 1327645

ACCCCGTGCTGGTGGAGAGAACTACAAGGAGTTCAGTGAGCTGCTCCCCA

ATCGACAGGGCCTGAAGAAAGCCGACTGCTCCTTCTGGTCCAAGTACATC

TCGTCTCTGAAGACATCTGCAGATGGAGCCAAGGGCGGGCAGTCAGCAGA

GAGTGAAGAGGAGGAGTTGACGGCTGGATCTGGGCTAAGAGAAGATCTC

CTAAGCCTCCAGGAACCAGGCTCTAAGACCTACAGCAAGTGACCAGCCCT

TGAGCTCCCCAAAAACCTCACCCGAGGCTGCCCACTATGGTCATCTTTTTC

TCTAAAATAGTTACTTACCTTCAATAAAGTATCTACATGCGGTG Sequence No. 99 Transforming growth factor, pi >gi I 10863872 I ref I NM_000660.1 I Human transformation growth factor

Sub, βΐ (Camurati-Engelmann) (TGFB1), mRNA |9卩€11 Advance_Introduction with [1651..1668]|4?€11 Reverse_Introduction and [1539..1557 ] Matching 丨qPCR probe matches [1687..1713]

ACCTCCCTCCGCGGAGCAGCCAGACAGCGAGGGCCCCGGCCGGGG

GCAGGGGGGACGCCCCGTCCGGGGCACCCCCCCCGGCTCTGAGCCGCCCG

CGGGGCCGGCCTCGGCCCGGAGCGGAGGAAGGAGTCGCCGAGGAGCAGC

CTGAGGCCCCAGAGTCTGAGACGAGCCGCCGCCGCCCCCGCCACTGCGGG

GAGGAGGGGGAGGAGGAGCGGGAGGAGGGACGAGCTGGTCGGGAGAAG

AGGAAAAAAACTTTTGAGACTTTTCCGTTGCCGCTGGGAGCCGGAGGCGC

GGGGACCTCTTGGCGCGACGCTGCCCCGCGAGGAGGCAGGACTTGGGGAC

CCCAGACCGCCTCCCTTTGCCGCCGGGGACGCTTGCTCCCTCCCTGCCCCC

TACACGGCGTCCCTCAGGCGCCCCCATTCCGGACCAGCCCTCGGGAGTCG

CCGACCCGGCCTCCCGCAAAGACTTTTCCCCAGACCTCGGGCGCACCCCCT

GCACGCCGCCTTCATCCCCGGCCTGTCTCCTGAGCCCCCGCGCATCCTAGA

CCCTTTCTCCTCCAGGAGACGGATCTCTCTCCGACCTGCCACAGATCCCCT

ATTCAAGACCACCCACCTTCTGGTACCAGATCGCGCCCATCTAGGTTATTT

CCGTGGGATACTGAGACACCCCCGGTCCAAGCCTCCCCTCCACCACTGCG

CCCTTCTCCCTGAGGAGCCTCAGCTTTCCCTCGAGGCCCTCCTACCTTTTGC

CGGGAGACCCCCAGCCCCTGCAGGGGCGGGGCCTCCCCACCACACCAGCC

CTGTTCGCGCTCTCGGCAGTGCCGGGGGGCGCCGCCTCCCCCATGCCGCCC

TCCGGGCTGCGGCTGCTGCCGCTGCTGCTACCGCTGCTGTGGCTACTGGTG

CTGACGCCTGGCCCGCCGGCCGCGGGACTATCCACCTGCAAGACTATCGA

CATGGAGCTGGTGAAGCGGAAGCGCATCGAGGCCATCCGCGGCCAGATCC

TGTCCAAGCTGCGGCTCGCCAGCCCCCCGAGCCAGGGGGAGGTGCCGCCC

GGCCCGCTGCCCGAGGCCGTGCTCGCCCTGTACAACAGCACCCGCGACCG

GGTGGCCGGGGAGAGTGCAGAACCGGAGCCCGAGCCTGAGGCCGACTAC

TACGCCAAGGAGGTCACCCGCGTGCTAATGGTGGAAACCCACAACGAAAT

CTATGACAAGTTCAAGCAGAGTACACACAGCATATATATGTTCTTCAACA 95238.doc -75- 1327645

CATCAGAGCTCCGAGAAGCGGTACCTGAACCCGTGTTGCTCTCCCGGGCA

GAGCTGCGTCTGCTGAGGAGGCTCAAGTTAAAAGTGGAGCAGCACGTGGA

GCTGTACCAGAAATACAGCAACAATTCCTGGCGATACCTCAGCAACCGGC

TGCTGGCACCCAGCGACTCGCCAGAGTGGTTATCTTTTGATGTCACCGGAG

TTGTGCGGCAGTGGTTGAGCCGTGGAGGGGAAATTGAGGGCTTTCGCCTT

AGCGCCCACTGCTCCTGTGACAGCAGGGATAACACACTGCAAGTGGACAT

CAACGGGTTCACTACCGGCCGCCGAGGTGACCTGGCCACCATTCATGGCA

TGAACCGGCCTTTCCTGCTTCTCATGGCCACCCCGCTGGAGAGGGCCCAGC

ATCTGCAAAGCTCCCGGCACCGCCGAGCCCTGGACACCAACTATTGCTTC

AGCTCCACGGAGAAGAACTGCTGCGTGCGGCAGCTGTACATTGACTTCCG

CAAGGACCTCGGCTGGAAGTGGATCCACGAGCCCAAGGGCTACCATGCCA

ACTTCTGCCTCGGGCCCTGCCCCTACATTTGGAGCCTGGACACGCAGTACA

GCAAGGTCCTGGCCCTGTACAACCAGCATAACCCGGGCGCCTCGGCGGCG

CCGTGCTGCGTGCCGCAGGCGCTGGAGCCGCTGCCCATCGTGTACTACGT

GGGCCGCAAGCCCAAGGTGGAGCAGCTGTCCAACATGATCGTGCGCTCCT

GCAAGTGCAGCTGAGGTCCCGCCCCGCCCCGCCCCGCCCCGGCAGGCCCG

GCCCCACCCCGCCCCGCCCCCGCTGCCTTGCCCATGGGGGCTGTATTTAAG

GACACCGTGCCCCAAGCCCACCTGGGGCCCCATTAAAGATGGAGAGAGG

ACTGCGGATCTCTGTGTCATTGGGCGCCTGCCTGGGGTCTCCATCCCTGAC

GTTCCCCCACTCCCACTCCCTCTCTCTCCCTCTCTGCCTCCTCCTGCCTGTC

TGCACTATTCCTTTGCCCGGCATCAAGGCACAGGGGACCAGTGGGGAACA

CTACTGTAGTTAGATCTATTTATTGAGCACCTTGGGCACTGTTGAAGTGCC

TTACATTAATGAACTCATTCAGTCACCATAGCAACACTCTGAGATGGCAG

GGACTCTGATAACACCCATTTTAAAGGTTGAGGAAACAAGCCCAGAGAGG

TTAAGGGAGGAGTTCCTGCCCACCAGGAACCTGCTTTAGTGGGGGATAGT

GAAGAAGACAATAAAAGATAGTAGTTCAGGCCAGGCGGGGTGCTCACGC

CTGTAATCCTAGCACTTTTGGGAGGCAGAGATGGGAGGATACTTGAATCC

AGGCATTTGAGACCAGCCTGGGTAACATAGTGAGACCCTATCTCTACAAA

ACACTTTTAAAAAATGTACACCTGTGGTCCCAGCTACTCTGGAGGCTAAG

GTGGGAGGATCACTTGATCCTGGGAGGTCAAGGCTGCAG Sequence No. 100 serine protease inhibitor, grade Η, member 1 >gi | 3 2454740 | ref | ΝΜ_001235.2 | Human serine (or cysteine) protease inhibitor, grade Η (heat shock) Protein 47), member 1, (collagen binding protein 1) (SERPINH1), mRNA | qPCR is determined to match [184..208] when required in the context.

TCTTTGGCTTTTTTTGGCGGAGCTGGGGCGCCCTCCGGAAGCGTTTC

CAACTTTCCAGAAGTTTCTCGGGACGGGCAGGAGGGGGTGGGGACTGCCA 95238.doc -76- 1327645

TATATAGATCCCGGGAGCAGGGGAGCGGGCTAAGAGTAGAATCGTGTCGC

GGCTCGAGAGCGAGAGTCACGTCCCGGCGCTAGCCCAGCCCGACCCAGGC

CCACCGTGGTGCACGCAAACCACTTCCTGGCCATGCGCTCCCTCCTGCTTC

TCAGCGCCTTCTGCCTCCTGGAGGCGGCCCTGGCCGCCGAGGTGAAGAAA

CCTGCAGCCGCAGCAGCTCCTGGCACTGCGGAGAAGTTGAGCCCCAAGGC

GGCCACGCTTGCCGAGCGCAGCGCCGGCCTGGCCTTCAGCTTGTACCAGG

CCATGGCCAAGGACCAGGCAGTGGAGAACATCCTGGTGTCACCCGTGGTG

GTGGCCTCGTCGCTAGGGCTCGTGTCGCTGGGCGGCAAGGCGACCACGGC

GTCGCAGGCCAAGGCAGTGCTGAGCGCCGAGCAGCTGCGCGACGAGGAG

GTGCACGCCGGCCTGGGCGAGCTGCTGCGCTCACTCAGCAACTCCACGGC

GCGCAACGTGACCTGGAAGCTGGGCAGCCGACTGTACGGACCCAGCTCAG

TGAGCTTCGCTGATGACTTCGTGCGCAGCAGCAAGCAGCACTACAACTGC

GAGCACTCCAAGATCAACTTCCGCGACAAGCGCAGCGCGCTGCAGTCCAT

CAACGAGTGGGCCGCGCAGACCACCGACGGCAAGCTGCCCGAGGTCACC

AAGGACGTGGAGCGCACGGACGGCGCCCTGCTAGTCAACGCCATGTTCTT

CAAGCCACACTGGGATGAGAAATTCCACCACAAGATGGTGGACAACCGTG

GCTTCATGGTGACTCGGTCCTATACCGTGGGTGTCATGATGATGCACCGGA

CAGGCCTCTACAACTACTACGACGACGAGAAGGAAAAGCTGCAAATCGTG

GAGATGCCCCTGGCCCACAAGCTCTCCAGCCTCATCATCCTCATGCCCCAT

CACGTGGAGCCTCTCGAGCGCCTTGAAAAGCTGCTAACCAAAGAGCAGCT

GAAGATCTGGATGGGGAAGATGCAGAAGAAGGCTGTTGCCATCTCCTTGC

CCAAGGGTGTGGTGGAGGTGACCCATGACCTGCAGAAACACCTGGCTGGG

CTGGGCCTGACTGAGGCCATTGACAAGAACAAGGCCGACTTGTCACGCAT

GTCAGGCAAGAAGGACCTGTACCTGGCCAGCGTGTTCCACGCCACCGCCT

TTGAGTTGGACACAGATGGCAACCCCTTTGACCAGGACATCTACGGGCGC

GAGGAGCTGCGCAGCCCCAAGCTGTTCTACGCCGACCACCCCTTCATCTTC

CTAGTGCGGGACACCCAAAGCGGCTCCCTGCTATTCATTGGGCGCCTGGT

CCGGCCTAAGGGTGACAAGATGCGAGACGAGTTATAGGGCCTCAGGGTGC

ACACAGGATGGCAGGAGGCATCCAAAGGCTCCTGAGACACATGGGTGCT

ATTGGGGTTGGGGGGGAGGTGAGGTACCAGCCTTGGATACTCCATGGGGT

GGGGGTGGAAAAACAGACCGGGGTTCCCGTGTGCCTGAGCGGACCTTCCC

AGCTAGAATTCACTCCACTTGGACATGGGCCCCAGATACCATGATGCTGA

GCCCGGAAACTCCACATCCTGTGGGACCTGGGCCATAGTCATTCTGCCTGC

CCTGAAAGTCCCAGATCAAGCCTGCCTCAATCAGTATTCATATTTATAGCC

AGGTACCTTCTCACCTGTGAGACCAAATTGAGCTAGGGGGGTCAGCCAGC

CCTCTTCTGACACTAAAACACCTCAGCTGCCTCCCCAGCTCTATCCCAACC

TCTCCCAACTATAAAACTAGGTGCTGCAGCCCCTGGGACCAGGCACCCCC

AGAATGACCTGGCCGCAGTGAGGCGGATTGAGAAGGAGCTCCCAGGAGG

GGCTTCTGGGCAGACTCTGGTCAAGAAGCATCGTGTCTGGCGTTGTGGGG

ATGAACTTTTTGTTTTGTTTCTTCCTTTTTTAGTTCTTCAAAGATAGGGAGG

GAAGGGGGAACATGAGCCTTTGTTGCTATCAATCCAAGAACTTATTTGTA

CATTTTTTTTTTCAATAAAACTTTTCCAATGACATTTTGTTGGAGCGTGGAA AAAA Sequence No. 101 95238.doc -77- 1327645 Serine protease inhibitor, rank B, member 5 >gi丨4505788丨ref | ΝΜ_002639·1 | Human serine (or cysteine) protease inhibition Agent, order Β (ovalbumin), member 5 (SERPINB5), mRNA 丨qPCR advance _ primer matches [36.·56] | 9? «1 reverse _ primer matches [106..86]|9? Ruler probe matches [60..80]

GGCACGAGTTGTGCTCCTCGCTTGCCTGTTCCTTTTCCGCATTT

CCAGGATAACTGTGACTCCAGGCCCGCAATGGATGCCCTGCAACTAGCAA

ATTCGGCTTTTGCCGTTGATCTGTTCAAACAACTATGTGAAAAGGAGCCAC

TGGGCAATGTCCTCTTCTCTCCAATCTGTCTCTCCACCTCTCTGTCACTTGC

TCAAGTGGGTGCTAAAGGTGACACTGCAAATGAAATTGGACAGGTTCTTC

ATTTTGAAAATGTCAAAGATATACCCTTTGGATTTCAAACAGTAACATCGG

ATGTAAACAAACTTAGTTCCTTTTACTCACTGAAACTAATCAAGCGGCTCT

ACGTAGACAAATCTCTGAATCTTTCTACAGAGTTCATCAGCTCTACGAAGA

GACCCTATGCAAAGGAATTGGAAACTGTTGACTTCAAAGATAAATTGGAA

GAAACGAAAGGTCAGATCAACAACTCAATTAAGGATCTCACAGATGGCCA

CTTTGAGAACATTTTAGCTGACAACAGTGTGAACGACCAGACCAAAATCC

TTGTGGTTAATGCTGCCTACTTTGTTGGCAAGTGGATGAAGAAATTTCCTG

AATCAGAAACAAAAGAATGTCCTTTCAGACTCAACAAGACAGACACCAA

ACCAGTGCAGATGATGAACATGGAGGCCACGTTCTGTATGGGAAACATTG

ACAGTATCAATTGTAAGATCATAGAGCTTCCTTTTCAAAATAAGCATCTCA

GCATGTTCATCCTACTACCCAAGGATGTGGAGGATGAGTCCACAGGCTTG

GAGAAGATTGAAAAACAACTCAACTCAGAGTCACTGTCACAGTGGACTAA

TCCCAGCACCATGGCCAATGCCAAGGTCAAACTCTCCATTCCAAAATTTA

AGGTGGAAAAGATGATTGATCCCAAGGCTTGTCTGGAAAATCTAGGGCTG

AAACATATCTTCAGTGAAGACACATCTGATTTCTCTGGAATGTCAGAGAC

CAAGGGAGTGGCCCTATCAAATGTTATCCACAAAGTGTGCTTAGAAATAA

CTGAAGATGGTGGGGATTCCATAGAGGTGCCAGGAGCACGGATCCTGCAG

CACAAGGATGAATTGAATGCTGACCATCCCTTTATTTACATCATCAGGCAC

AACAAAACTCGAAACATCATTTTCTTTGGCAAATTCTGTTCTCCTTAAGTG

GCATAGCCCATGTTAAGTCCTCCCTGACTTTTCTGTGGATGCCGATTTCTG

TAAACTCTGCATCCAGAGATTCATTTTCTAGATACAATAAATTGCTAATGT

TGCTGGATCAGGAAGCCGCCAGTACTTGTCATATGTAGCCTTCACACAGA

TAGACCTTTTTTTTTTTCCAATTCTATCTTTTGTTTCCTTTTTTCCCATAAGA

CAATGACATACGCTTTTAATGAAAAGGAATCACGTTAGAGGAAAAATATT

TATTCATTATTTGTCAAATTGTCCGGGGTAGTTGGCAGAAATACAGTCTTC

CACAAAGAAAATTCCTATAAGGAAGATTTGGAAGCTCTTCTTCCCAGCAC

TATGCTTTCCTTCTTTGGGATAGAGAATGTTCCAGACATTCTCGCTTCCCTG

AAAGACTGAAGAAAGTGTAGTGCATGGGACCCACGAAACTGCCCTGGCTC 95238.doc •78- 1327645

CAGTGAAACTTGGGCACATGCTCAGGCTACTATAGGTCCAGAAGTCCTTA

TGTTAAGCCCTGGCAGGCAGGTGTTTATTAAAATTCTGAATTTTGGGGATT

TTCAAAAGATAATATTTTACATACACTGTATGTTATAGAACTTCATGGATC

AGATCTGGGGCAGCAACCTATAAATCAACACCTTAATATGCTGCAACAAA

ATGTAGAATATTCAGACAAAATGGATACATAAAGACTAAGTAGCCCATAA

GGGGTCAAAATTTGCTGCCAAATGCGTATGCCACCAACTTACAAAAACAC

TTCGTTCGCAGAGCTTTTCAGATTGTGGAATGTTGGATAAGGAATTATAGA

CCTCTAGTAGCTGAAATGCAAGACCCCAAGAGGAAGTTCAGATCTTAATA

TAAATTCACTTTCATTTTTGATAGCTGTCCCATCTGGTCATGTGGTTGGCAC

TAGACTGGTGGCAGGGGCTTCTAGCTGACTC-GCACAGGGATTCTCACAAT

AGCCGATATCAGAATTTGTGTTGAAGGAACTTGTCTCTTCATCTAATATGA

TAGCGGGAAAAGGAGAGGAAACTACTGCCTTTAGAAAATATAAGTAAAG

TGATTAAAGTGCTCACGTTACCTTGACACATAGTTTTTCAGTCTATGGGTT

TAGTTACTTTAGATGGCAAGCATGTAACTTATATTAATAGTAATTTGTAAA

GTTGGGTGGATAAGCTATCCCTGTTGCCGGTTCATGGATTACTTCTCTATA

AAAAATATATATTTACCAAAAAATTTTGTGACATTCCTTCTCCCATCTCTT

CCTTGACATGCATTGTAAATAGGTTCTTCTTGTTCTGAGATTCAATATTGA

ATTTCTCCTATGCTATTGACAATAAAATATTATTGAACTACC Sequence No. 102 carcinoembryonic antigen-related cell adhesion molecule 5 >gi | 11386170 | ref | NM—004363.1 丨 Human carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5), mRNA 丨qPCR in the context Measured at the time of request and matched with [2128..2 152]

CTCAGGGCAGAGGGAGGAAGGACAGCAGACCAGACAGTCACAGC

AGCCTTGACAAAACGTTCCTGGAACTCAAGCTCTTCTCCACAGAGGAGGA

CAGAGCAGACAGCAGAGACCATGGAGTCTCCCTCGGCCCCTCCCCACAGA

TGGTGCATCCCCTGGCAGAGGCTCCTGCTCACAGCCTCACTTCTAACCTTC

TGGAACCCGCCCACCACTGCCAAGCTCACTATTGAATCCACGCCGTTCAAT

GTCGCAGAGGGGAAGGAGGTGCTTCTACTTGTCCACAATCTGCCCCAGCA

TCTTTTTGGCTACAGCTGGTACAAAGGTGAAAGAGTGGATGGCAACCGTC

AAATTATAGGATATGTAATAGGAACTCAACAAGCTACCCCAGGGCCCGCA

TACAGTGGTCGAGAGATAATATACCCCAATGCATCCCTGCTGATCCAGAA

CATCATCCAGAATGACACAGGATTCTACACCCTACACGTCATAAAGTCAG

ATCTTGTGAATGAAGAAGCAACTGGCCAGTTCCGGGTATACCCGGAGCTG

CCCAAGCCCTCCATCTCCAGCAACAACTCCAAACCCGTGGAGGACAAGGA

TGCTGTGGCCTTCACCTGTGAACCTGAGACTCAGGACGCAACCTACCTGTG

GTGGGTAAACAATCAGAGCCTCCCGGTCAGTCCCAGGCTGCAGCTGTCCA

ATGGCAACAGGACCCTCACTCTATTCAATGTCACAAGAAATGACACAGCA

AGCTACAAATGTGAAACCCAGAACCCAGTGAGTGCCAGGCGCAGTGATTC 95238.doc •79· 1327645

AGTCATCCTGAATGTCCTCTATGGCCCGGATGCCCCCACCATTTCCCCTCT

AAACACATCTTACAGATCAGGGGAAAATCTGAACCTCTCCTGCCACGCAG

CCTCTAACCCACCTGCACAGTACTCTTGGTTTGTCAATGGGACTTTCCAGC

AATCCACCCAAGAGCTCTTTATCCCCAACATCACTGTGAATAATAGTGGAT

CCTATACGTGCCAAGCCCATAACTCAGACACTGGCCTCAATAGGACCACA

GTCACGACGATCACAGTCTATGCAGAGCCACCCAAACCCTTCATCACCAG

CAACAACTCCAACCCCGTGGAGGATGAGGATGCTGTAGCCTTAACCTGTG

AACCTGAGATTCAGAACACAACCTACCTGTGGTGGGTAAATAATCAGAGC

CTCCCGGTCAGTCCCAGGCTGCAGCTGTCCAATGACAACAGGACCCTCAC

TCTACTCAGTGTCACAAGGAATGATGTAGGACCCTATGAGTGTGGAATCC

AGAACGAATTAAGTGTTGACCACAGCGACCCAGTCATCCTGAATGTCCTC

TATGGCCCAGACGACCCCACCATTTCCCCCTCATACACCTATTACCGTCCA

GGGGTGAACCTCAGCCTCTCCTGCCATGCAGCCTCTAACCCACCTGCACA

GTATTCTTGGCTGATTGATGGGAACATCCAGCAACACACACAAGAGCTCT

TTATCTCCAACATCACTGAGAAGAACAGCGGACTCTATACCTGCCAGGCC

AATAACTCAGCCAGTGGCCACAGCAGGACTACAGTCAAGACAATCACAGT

CTCTGCGGAGCTGCCCAAGCCCTCCATCTCCAGCAACAACTCCAAACCCG

TGGAGGACAAGGATGCTGTGGCCTTCACCTGTGAACCTGAGGCTCAGAAC

ACAACCTACCTGTGGTGGGTAAATGGTCAGAGCCTCCCAGTCAGTCCCAG

GCTGCAGCTGTCCAATGGCAACAGGACCCTCACTCTATTCAATGTCACAA

GAAATGACGCAAGAGCCTATGTATGTGGAATCCAGAACTCAGTGAGTGCA

AACCGCAGTGACCCAGTCACCCTGGATGTCCTCTATGGGCCGGACACCCC

CATCATTTCCCCCCCAGACTCGTCTTACCTTTCGGGAGCGAACCTCAACCT

CTCCTGCCACTCGGCCTCTAACCCATCCCCGCAGTATTCTTGGCGTATCAA

TGGGATACCGCAGCAACACACACAAGTTCTCTTTATCGCCAAAATCACGC

CAAATAATAACGGGACCTATGCCTGTTTTGTCTCTAACTTGGCTACTGGCC

GCAATAATTCCATAGTCAAGAGCATCACAGTCTCTGCATCTGGAACTTCTC

CTGGTCTCTCAGCTGGGGCCACTGTCGGCATCATGATTGGAGTGCTGGTTG

GGGTTGCTCTGATATAGCAGCCCTGGTGTAGTTTCTTCATTTCAGGAAGAC

TGACAGTTGTTTTGCTTCTTCCTTAAAGCATTTGCAACAGCTACAGTCTAA

AATTGCTTCTTTACCAAGGATATTTACAGAAAAGACTCTGACCAGAGATC

GAGACCATCCTAGCCAACATCGTGAAACCCCATCTCTACTAAAAATACAA

AAATGAGCTGGGCTTGGTGGCGCGCACCTGTAGTCCCAGTTACTCGGGAG

GCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGTGGAGATTGCAGTGAG

CCCAGATCGCACCACTGCACTCCAGTCTGGCAACAGAGCAAGACTCCATC

TCAAAAAGAAAAGAAAAGAAGACTCTGACCTGTACTCTTGAATACAAGTT

TCTGATACCACTGCACTGTCTGAGAATTTCCAAAACTTTAATGAACTAACT

GACAGCTTCATGAAACTGTCCACCAAGATCAAGCAGAGAAAATAATTAAT

TTCATGGGACTAAATGAACTAATGAGGATTGCTGATTCTTTAAATGTCTTG

TTTCCCAGATTTCAGGAAACTTTTTTTCTTTTAAGCTATCCACTCTTACAGC

AATTTGATAAAATATACTTTTGTGAACAAAAATTGAGACATTTACATTTTC

TCCCTATGTGGTCGCTCCAGACTTGGGAAACTATTCATGAATATTTATATT

GTATGGTAATATAGTTATTGCACAAGTTCAATAAAAATCTGCTCTTTGTAT 95238.doc •80-1327645 AACAGAAAAA Sequence No. 103 Matrix Metalloproteinase 2 >gi 丨11342665 | ref | NM_004530.1 | Human Matrix Metalloproteinase 2 (Gelatinase A, 72 kDa Gelatinase, 72 kDa Type IV Collagen Protease) (MMP2), mRNA|qPCR Advance_Introduction matches [1713..1732] | qPCR reverse_bow|submatch with [1793..1775] | qPCR probe matches [1751·.1773]

TGTTTCCGCTGCATCCAGACTTCCTCAGGCGGTGGCTGGAGGCTGC

GCATCTGGGGCTTTAAACATACAAAGGGATTGCCAGGACCTGCGGCGGCG

GCGGCGGCGGCGGGGGCTGGGGCGCGGGGGCCGGACCATGAGCCGCTGA

GCCGGGCAAACCCCAGGCCACCGAGCCAGCGGACCCTCGGAGCGCAGCC

CTGCGCCGCGGACCAGGCTCCAACCAGGCGGCGAGGCGGCCACACGCAC

CGAGCCAGCGACCCCCGGGCGACGCGCGGGGCCAGGGAGCGCTACGATG

GAGGCGCTAATGGCCCGGGGCGCGCTCACGGGTCCCCTGAGGGCGCTCTG

TCTCCTGGGCTGCCTGCTGAGCCACGCCGCCGCCGCGCCGTCGCCCATCAT

CAAGTTCCCCGGCGATGTCGCCCCCAAAACGGACAAAGAGTTGGCAGTGC

AATACCTGAACACCTTCTATGGCTGCCCCAAGGAGAGCTGCAACCTGTTT

GTGCTGAAGGACACACTAAAGAAGATGCAGAAGTTCTTTGGACTGCCCCA

GACAGGTGATCTTGACCAGAATACCATCGAGACCATGCGGAAGCCACGCT

GCGGCAACCCAGATGTGGCCAACTACAACTTCTTCCCTCGCAAGCCCAAG

TGGGACAAGAACCAGATCACATACAGGATCATTGGCTACACACCTGATCT

GGACCCAGAGACAGTGGATGATGCCTTTGCTCGTGCCTTCCAAGTCTGGA

GCGATGTGACCCCACTGCGGTTTTCTCGAATCCATGATGGAGAGGCAGAC

ATCATGATCAACTTTGGCCGCTGGGAGCATGGCGATGGATACCCCTTTGA

CGGTAAGGACGGACTCCTGGCTCATGCCTTCGCCCCAGGCACTGGTGTTG

GGGGAGACTCCCATTTTGATGACGATGAGCTATGGACCTTGGGAGAAGGC

CAAGTGGTCCGTGTGAAGTATGGCAACGCCGATGGGGAGTACTGCAAGTT

CCCCTTCTTGTTCAATGGCAAGGAGTACAACAGCTGCACTGATACTGGCC

GCAGCGATGGCTTCCTCTGGTGCTCCACCACCTACAACTTTGAGAAGGAT

GGCAAGTACGGCTTCTGTCCCCATGAAGCCCTGTTCACCATGGGCGGCAA

CGCTGAAGGACAGCCCTGCAAGTTTCCATTCCGCTTCCAGGGCACATCCTA

TGACAGCTGCACCACTGAGGGCCGCACGGATGGCTACCGCTGGTGCGGCA

CCACTGAGGACTACGACCGCGACAAGAAGTATGGCTTCTGCCCTGAGACC

GCCATGTCCACTGTTGGTGGGAACTCAGAAGGTGCCCCCTGTGTCTTCCCC

TTCACTTTCCTGGGCAACAAATATGAGAGCTGCACCAGCGCCGGCCGCAG

TGACGGAAAGATGTGGTGTGCGACCACAGCCAACTACGATGACGACCGCA

AGTGGGGCTTCTGCCCTGACCAAGGGTACAGCCTGTTCCTCGTGGCAGCC 95238.doc -81 - 1327645

CACGAGTTTGGCCACGCCATGGGGCTGGAGCACTCCCAAGACCCTGGGGC

CCTGATGGCACCCATTTACACCTACACCAAGAACTTCCGTCTGTCCCAGGA

TGACATCAAGGGCATTCAGGAGCTCTATGGGGCCTCTCCTGACATTGACCT

TGGCACCGGCCCCACCCCCACACTGGGCCCTGTCACTCCTGAGATCTGCA

AACAGGACATTGTATTTGATGGCATCGCTCAGATCCGTGGTGAGATCTTCT

TCTTCAAGGACCGGTTCATTTGGCGGACTGTGACGCCACGTGACAAGCCC

ATGGGGCCCCTGCTGGTGGCCACATTCTGGCCTGAGCTCCCGGAAAAGAT

TGATGCGGTATACGAGGCCCCACAGGAGGAGAAGGCTGTGTTCTTTGCAG

GGAATGAATACTGGATCTACTCAGCCAGCACCCTGGAGCGAGGGTACCCC

AAGCCACTGACCAGCCTGGGACTGCCCCCTGATGTCCAGCGAGTGGATGC

CGCCTTTAACTGGAGCAAAAACAAGAAGACATACATCTTTGCTGGAGACA

AATTCTGGAGATACAATGAGGTGAAGAAGAAAATGGATCCTGGCTTTCCC

AAGCTCATCGCAGATGCCTGGAATGCCATCCCCGATAACCTGGATGCCGT

CGTGGACCTGCAGGGCGGCGGTCACAGCTACTTCTTCAAGGGTGCCTATT

ACCTGAAGCTGGAGAACCAAAGTCTGAAGAGCGTGAAGTTTGGAAGCATC

AAATCCGACTGGCTAGGCTGCTGAGCTGGCCCTGGCTCCCACAGGCCCTT

CCTCTCCACTGCCTTCGATACACCGGGCCTGGAGAACTAGAGAAGGACCC

GGAGGGGCCTGGCAGCCGTGCCTTCAGCTCTACAGCTAATCAGCATTCTC actcctacctggtaatttaagattccagagagtggctcctcccggtgccca AGAATAGATGCTGACTGTACTCCTCCCAGGCGCCCCTTCCCCCTCCAATCC CACCAACCCTCAGAGCCACCCCTAAAGAGATCCTTTGATATTTTCAACGCA GCCCTGCTTTGGGCTGCCCTGGTGCTGCCACACTTCAGGCTCTTCTCCTTTC ACAACCTTCTGTGGCTCACAGAACCCTTGGAGCCAATGGAGACTGTCTCA AGAGGGCACTGGTGGCCCGACAGCCTGGCACAGGGCAGTGGGACAGGGC ATGGCCAGGTGGCCACTCCAGACCCCTGGCTTTTCACTGCTGGCTGCCTTA GAACCTTTCTTACATTAGCAGTTTGCTTTGTATGCACTTTGTTTTTTTCTTT GGGTCTTGTTTTTTTTTTCCACTTAGAAATTGCATTTCCTGACAGAAGGACT CAGGTTGTCTGAAGTCACTGCACAGTGCATCTCAGCCCACATAGTGATGG TTCCCCTGTTCACTCTACTTAGCATGTCCCTACCGAGTCTCTTCTCCACTGG ATGGAGGAAAACCAAGCCGTGGCTTCCCGCTCAGCCCTCCCTGCCCCTCCT TCAACCATTCCCCATGGGAAATGTCAACAAGTATGAATAAAGACACCTAC TGAGTGGC serial No. 104 proprotein convertase subtilisin / Regents Type 5 > gi | 20336245 | ref | ΝΜ_006200 · 2 | human proprotein convertase subtilisin Bacillusin/Kaixin Type 5 (PCSK5), mRNA | qPCR Advance_Introduction to [2677..2697] | qPCR Reverse-Introduction and [2821..28 01] Matching | qPCR probe matches [2737..2765]

CGGAGGGAGCGCTGGGAGCGAGCAAGCGAGCGTTTGGAGCCCGGG 95238.doc • 82 · 1327645

CCAGCAGAGGGGGCGCCCGGTCGCTGCCTGTACCGCTCCCGCTGGTCATC

TCCGCCGCGCTCGGGGGCCCCGGGAGGAGCGAGACCGAGTCGGAGAGTC

CGGGAGCCAAGCCGGGCGAAACCCAACTGCGGAGGACGCCCGCCCCACT

CAGCCTCCTCCTGCGTCCGAGCCGGGGAGCATCGCCGAGCGCCCCACGGG

CCGGAGAGCTGGGAGCACAGGTCCGGGCAGCCCCAGGGATGGTCTAGGA

GCCGGCGTAAGGCTCGCTGCTCTGCTCCCTGCCGGGGCTAGCCGCCTCCTG

CCGATCGCCCGGGGCTGCGAGCTGCGGCGGCCCGGGGCTGCTCGCCGGGC

GGCGCAGGCCGGAGAAGTTAGTTGTGCGCGCCCTTAGTGCGCGGAACCAG

CCAGCGAGCGAGGGAGCAGCGAGGCGCCGGGACCATGGGCTGGGGGAGC

CGCTGCTGCTGCCCGGGACGTTTGGACCTGCTGTGCGTGCTGGCGCTGCTC

GGGGGCTGCCTGCrCCCCGTGTGTCGGACGCGCGTCTACACCAACCACTG

GGCAGTCAAAATCGCCGGGGGCTTCCCGGAGGCCAACCGTATCGCCAGCA

AGTACGGATTCATCAACATAGGACAGATAGGGGCCCTGAAGGACTACTAC

CACTTCTACCATAGCAGGACGATTAAAAGGTCAGTTATCTCGAGCAGAGG

GACCCACAGTTTCATTTCAATGGAACCAAAGGTGGAATGGATCCAACAGC

AAGTGGTAAAAAAGCGGACAAAGAGGGATTATGACTTCAGTCGTGCCCA

GTCTACCTATTTCAATGATCCCAAGTGGCCCAGCATGTGGTATATGCACTG

CAGTGACAATACACATCCCTGCCAGTCTGACATGAATATCGAAGGAGCCT

GGAAGAGAGGCTACACGGGAAAGAACATTGTGGTCACTATCCTGGATGAC

GGAATTGAGAGAACCCATCCAGATCTGATGCAAAACTACGATGCTCTGGC

AAGTTGCGACGTGAATGGGAATGACTTGGACCCAATGCCTCGTTATGATG

CAAGCAACGAGAACAAGCATGGGACTCGCTGTGCTGGAGAAGTGGCAGC

CGCTGCAAACAATTCGCACTGCACAGTCGGAATTGCTTTCAACGCCAAGA

TCGGAGGAGTGCGAATGCTGGACGGAGATGTCACGGACATGGTTGAAGC

AAAATCAGTTAGCTTCAACCCCCAGCACGTGCACATTTACAGCGCCAGCT

GGGGCCCGGATGATGATGGCAAGACTGTGGACGGACCAGCCCCCCTCACC

CGGCAAGCCTTTGAAAACGGCGTTAGAATGGGGCGGAGAGGCCTCGGCTC

TGTGTTTGTTTGGGCATCTGGAAATGGTGGAAGGAGCAAAGACCACTGCT

CCTGTGATGGCTACACCAACAGCATCTACACCATCTCCATCAGCAGCACT

GCAGAAAGCGGAAAGAAACCTTGGTACCTGGAAGAGTGTTCATCCACGCT

GGCCACAACCTACAGCAGCGGGGAGTCCTACGATAAGAAAATCATCACTA

CAGATCTGAGGCAGCGTTGCACGGACAACCACACTGGGACGTCAGCCTCA

GCCCCCATGGCTGCAGGCATCATTGCGCTGGCCCTGGAAGCCAATCCGTTT

CTGACCTGGAGAGACGTACAGCATGTTATTGTCAGGACTTCCCGTGCGGG

ACATTTGAACGCTAATGACTGGAAAACCAATGCTGCTGGTTTTAAGGTGA

GCCATCTTTATGGATTTGGACTGATGGACGCAGAAGCCATGGTGATGGAG

GCAGAGAAGTGGACCACCGTTCCCCGGCAGCACGTGTGTGTGGAGAGCAC

AGACCGACAAATCAAGACAATCCGCCCTAACAGTGCAGTGCGCTCCATCT

ACAAAGCTTCAGGCTGCTCGGATAACCCCAACCGCCATGTCAACTACCTG

GAGCACGTCGTTGTGCGCATCACCATCACCCACCCCAGGAGAGGAGACCT

GGCCATCTACCTGACCTCGCCCTCTGGAACTAGGTCTCAGCTTTTGGCCAA

CAGGCTATTTGATCACTCCATGGAAGGATTCAAAAACTGGGAGTTCATGA

CCATTCATTGCTGGGGAGAAAGAGCTGCTGGTGACTGGGTCCTTGAAGTT 95238.doc _ 83 · 1327645 TATGATACTCCCTCTCAGCTAAGGAACTTTAAGACTCCAGGTAAATTGAA AGAATGGTCTTTGGTCCTCTACGGCACCTCCGTGCAGCCATATTCACCAAC CAATGAATTTCCGAAAGTGGAACGGTTCCGCTATAGCCGAGTTGAAGACC CCACAGACGACTATGGCACAGAGGATTATGCAGGTCCCTGCGACCCTGAG TGCAGTGAGGTTGGCTGTGACGGGCCAGGACCAGACCACTGCAATGACTG TTTGCACTACTACTACAAGCTGAAAAACAATACCAGGATCTGTGTCTCCA GCTGCCCCCCTGGCCACTACCACGCCGACAAGAAGCGCTGCAGGAAGTGT GCCCCCAACTGTGAGTCCTGCTTTGGGAGCCATGGTGACCAATGCATGTCC TGCAAATATGGATACTTTCTGAATGAAGAAACCAACAGCTGTGTTACTCA CTGCCCTGATGGGTCATATCAGGATACCAAGAAAAATCTTTGCCGGAAAT GCAGTGAAAACTGCAAGACATGTACTGAATTCCATAACTGTACAGAATGT AGGGATGGGTTAAGCCTGCAGGGATCCCGGTGCTCTGTCTCCTGTGAAGA TGGACGGTATTTCAACGGCCAGGACTGCCAGCCCTGCCACCGCTTCTGCG CCACTTGTGCTGGGGCAGGAGCTGATGGGTGCATTAACTGCACAGAGGGC TACTTCATGGAGGATGGGAGATGCGTGCAGAGCTGTAGTATCAGCTATTA CTTTGACCACTCTTCAGAGAATGGATACAAATCCTGCAAAAAATGTGATA TCAGTTGTTTGACGTGCAATGGCCCAGGATTCAAGAACTGTACAAGCTGC CCTAGTGGGTATCTCTTAGACTTAGGAATGTGTCAAATGGGAGCCATTTGC AA GGATGCAACGGAAGAGTCCTGGGCGGAAGGAGGCTTCTGTATGCTTGT GAAAAAGAACAATCTGTGCCAACGGAAGGTTCTTCAACAACTTTGCTGCA AAACATGTACATTTCAAGGCTGAGCAGCCATCTTAGATTTCTTTGTTCCTG TAGACTTATAGATTATTCCATATTATTAAAAAGAAAAAAAAAAGCCAAAA AG Sequence No. 105

Carboxypeptidase N, peptide 2,83 kD

>gi 丨 18554966 I ref I XM_087358.1 I Human carboxypeptidase N, polypeptide 2,83 kD (CPN2), mRNA

ATGGGTTGTGACTGCTTCGTCCAGGAGGTGTTCTGCTCAGATGAGG

AGCTTGCCACCGTCCCGCTGGACATCCCGCCATATACGAAAAACATCATC

TTTGTGGAGACCTCGTTCACCACATTGGAAACCAGAGCTTTTGGCAGTAAC

CCCAACTTGACCAAGGTGGTCTTCCTCAACACTCAGCTCTGCCAGTTTAGG

CCGGATGCCTTTGGGGGGCTGCCCAGGCTGGAGGACCTGGAGGTCACAGG

CAGTAGCTTCTTGAACCTCAGCACCAACATCTTCTCCAACCTGACCTCGCT

GGGCAAGCTCACCCTCAACTTCAACATGCTGGAGGCTCTGCCCGAGGGTC

TTTTCCAGCACCTGGCTGCCCTGGAGTCCCTCCACCTGCAGGGGAACCAGC

TCCAGGCCCTGCCCAGGAGGCTCTTCCAGCCTCTGACCCATCTGAAGACA

CTCAACCTGGCCCAGAACCTCCTGGCCCAGCTCCCGGAGGAGCTGTTCCA

CCCACTCACCAGCCTGCAGACCCTGAAGCTGAGCAACAACGCGCTCTCTG

GTCTCCCCCAGGGTGTGTTTGGCAAACTGGGCAGCCTGCAGGAGCTCTTCC

TGGACAGCAACAACATCTCGGAGCTGCCCCCTCAGGTGTTCTCCCAGCTCT

TCTGCCTAGAGAGGCTGTGGCTGCAACGCAACGCCATCACGCACCTGCCG 95238.doc 84 · 1327645 CTCTCCATCTTTGCCTCCCTGGGTAATCTGACCTTTCTGAGCTTGCAGTGG AACATGCTTCGGGTCCTGCCTGCCGGCCTCTTTGCCCACACCCCATGCCTG GTTGGCCTGTCTCTGACCCATAACCAGCTGGAGACTGTCGCTGAGGGCAC CTTTGCCCACCTGTCCAACCTGCGTTCCCTCATGCTCTCATACAATGCCATT ACCCACCTCCCAGCTGGCATCTTCAGAGACCTGGAGGAGTTGGTCAAACT CTACCTGGGCAGCAACAACCTTACGGCGCTGCACCCAGCCCTCTTCCAGA ACCTGTCCAAGCTGGAGCTGCTCAGCCTCTCCAAGAACCAGCTGACCACA CTTCCGGAGGGCATCTTCGACACCAACTACAACCTGTTCAACCTGGCCCTG CACGGTAACCCCTGGCAGTGCGACTGCCACCTGGCCTACCTCTTCAACTGG CTGCAGCAGTACACCGATCGGCTCCTGAACATCCAGACCTACTGCGCTGG CCCTGCCTACCTCAAAGGCCAGGTGGTGCCCGCCTTGAATGAGAAGCAGC TGGTGTGTCCCGTCACCCGGGACCACTTGGGCTTCCAGGTCACGTGGCCG GACGAAAGCAAGGCAGGGGGCAGCTGGGATCTGGCTGTGCAGGAAAGGG CAGCCCGGAGCCAGTGCACCTACAGCAACCCCGAGGGCACCGTGGTGCTC GCCTGTGACCAGGCCCAGTGTCGCTGGCTGAACGTCCAGCTCTCTCCTTGG CAGGGCTCCCTGGGACTGCAGTACAATGCTAGTCAGGAGTGGGACCTGAG GTCGAGCTGCGGTTCTCTGCGGCTCACCGTGTCTATCGAGGCTCGGGCAGC AGGGCCCTAGTAGCAGCGCATACAGGAGCTGGGGAAGGGGGCTTTGGGG CC TGCCCACGCGACAGGTAGGGGCGGAGGGGAGCTGAGTCTCCGAAGCTT GGCTTT Sequence No. 106 Hyaluronic Acid and Proteoglycan Binding Protein 4 >gi | 30794471 | ref 丨 NM_023002.1 | Human Hyaluronic Acid and

Proteoglycan-linked protein 4 (HAPLN4), mRNA

CGGGGGCCGCGCGGGCAAGATGGTGTGCGCTCGGGCGGCCCTCGG

TCCCGGCGCGCTCTGGGCCGCGGCCTGGGGCGTCCTGCTGCTCACAGCCC

CTGCGGGGGCGCAGCGTGGCCGGAAGAAGGTCGTGCACGTGCTGGAGGG

TGAGTCGGGCTCGGTAGTGGTACAGACAGCGCCTGGGCAGGTGGTAAGCC

ACCGTGGTGGCACCATCGTCTTGCCCTGCCGCTACCACTATGAGGCAGCC

GCCCACGGTCACGACGGCGTCCGGCTCAAGTGGACAAAGGTGGTGGACCC

GCTGGCCTTCACCGACGTCTTCGTGGCACTAGGCCCCCAGCACCGGGCATT

CGGCAGCTACCGTGGGCGGGCTGAGCTGCAGGGCGACGGGCCTGGGGAT

GCCTCCCTGGTCCTCCGCAACGTCACGCTGCAAGACTACGGGCGCTATGA

GTGCGAAGTCACCAATGAGCTGGAAGATGACGCTGGCATGGTCAAGCTGG

ACCTGGAAGGCGTGGTCTTTCCCTACCACCCCCGTGGAGGCCGATACAAG

CTGACCTTCGCGGAGGCGCAGCGCGCGTGCGCCGAGCAGGACGGCATCCT

GGCATCTGCAGAACAGCTGCACGCGGCCTGGCGCGACGGCCTGGACTGGT

GCAACGCGGGCTGGTTGCGCGACGGCTCAGTGCAATACCCCGTGAACCGG

CCCCGGGAGCCCTGCGGCGGCCTGGGGGGGACCGGGAGTGCAGGGGGCG

GCGGTGATGCCAACGGGGGCCTGCGCAACTACGGGTATCGCCATAACGCC

GAGGAACGCTACGACGCCTTCTGCTTCACGTCCAACCTGCCGGGGCGCGT 95238.doc •85· 1327645

GTTCTTCCTGAAGCCGCTGCGACCTGTACCCTTCTCCGGAGCTGCGCGCGC

GTGTGCTGCGCGTGGCGCGGCCGTGGCCAAGGTGGGGCAGCTGTTCGCCG

CGTGGAAGCTGCAGCTGCTAGACCGCTGCACCGCGGGTTGGCTGGCCGAT

GGCAGTGCGCGCTACCCCATCGTGAACCCGCGAGCGCGCTGCGGAGGCCG

CAGGCCTGGTGTGCGCAGCCTCGGCTTCCCGGACGCCACCCGACGGCTCT

TCGGCGTCTACTGCTACCGCGCTCCAGGAGCACCGGACCCGGCACCTGGC

GGCTGGGGCTGGGGCTGGGCGGGCGGCGGCGGCTGGGCAGGGGGCGCGC

GCGATCCTGCTGCCTGGACCCCTCTGCACGTCTAGGCTGGGAGTAGGCGG

ACAGCCAGGGCGCTTGACCACTGGTCTAGAGCCCTGTGGTCCCCTGGAGC

CTGGCCACGCCCTTGAAGCCCTGGACACTGGCCACATTCCCTGTGGTCCCT

TACAAACTAACTGTGCCCCTGGGGTCCCTGAAGACTGGCTAGTCCTGGCA

GAACAGTACTTTGGAGTTCCCTGGAGCCTGGCCAGCCCTCACCTCTTCTGG

ATAGAGGATTCCCCCAACTCCCCAACTTTCTCCATGAGGGTCACGCCCCCT

GAGGACCTCAGGAGGCCAGCAGAACCCGCAGGCTCCTGAAGACTGGCCA

CGCCTCCTGAGACCACTTGGAAACAGACCAACTGCCCCCGTGGTCGCCTG

GTGGCTGGACCCCCGGGATTGACTAGAGACCGGCCGTACACCTTCTGCAT

CTCACTGGAGACTGAACACTAGTCCCTTGCGGTCACGTGGGACACTGGGC

GCCTCCTCCTCCCCCTCCTCCTCACCTGGAGAGACTACAGGAACTTCAGGG

TCACTCCCCGTGGTCACATGGAGGTTGTGGGCCGAGGCGCTTATTTTCCCT

TATGGTGACCTGAGTCCTGGAGACTCCCATTCTCCCCCTCTCCCTGAGAGT

CCCCTGCAGTTTCTGGGTAACAGGGCACACCCCTCTAGTTTCATGGGCGAG

CACCCCCATCTGCCACCTCAGACTGACACACAGCCAGCTGGCTCACTTACT

GGGGGCCACGTCCCACCCCTCAGATATTTCTTTGAAGGGAGAGCAAACCC

ACCCTGTCCTCTGACGTCCCTTTCCCAACTGTCACCAAACAGACCATCTTC

CCAGGCCTGGGGACCGGTAAGATCCATGTCACTAGTTATGCAGAGCAGTT

GCCTTGGGTCCCACTGTCACCAAGGCAACCAGTCCTGCTGCTACCTGTCAC

CTAGAGTCACACACCCCTTCCCTCATCAGGCACACCCATGAAGACAGTGC

CTCCCTCCTCCAGCTGTAACCATGGATACCACACATTTCTCATCTCATTGG

CCCCCACCCCAGAGACCTCCACCTCAACTTCTGGCTGTCCCTACCCTGACT

CACCGCCATGGAGATCACCCTCCCCGAAGCTGTCGCCAGGGTGACCCAAC

ATCCAGTTCTCCGGCTCTCACCATGGAAACAAACTGTCCCTGTCCCCAGGC

CCACTCCAGTTCCAGACCACCCTCCATGCTCCACCCCCAGGCGGTTTGGAC

CCCACCACTGTTGCCATGGTGACCAAACTCTGGAGTCCGAGGTAACAGAA

CACCTGTCCCCCTAGGCTTTTCCTTGTGGACAACGGGGCCCTGTTCACCAA

GCTGTTGCCATAGAGACTGTCAACGTTGTCCTCATGACAACCAGACTTCCA

GTTCTCAGGAACTTCTCATTGTGGGCCAGAAGTCCTGGGTGCCTCCTACTA

GGGCTACCCTACTGCACCCCATCAGGGGCCTGATGGCTGCCCCTTCCCCAG

ACAGGGCTGGACTTCTGGAGCTGCTAAGCCACCCTCCGTTTGCACGTTAAC

TCTATGCCGGATAGCAGCTGTGCACGAGACAATCTTGCAACACCCGGGCA

TGTTTGTCGTCGTCCTACAAATGAGGAAACCGAGCCTATGGCGTGCCCTG

GTCTGTTGAGATATGCAAGCACTGAGCTCCTCTTTTGTCCTCTGAGACCCC

ATCTCCATTCTCACCCAGTTCCTCTCTCCTTCCCTGACCCCCACCCACATTT

CCCTCCTTAGAGATCCAGGAGGGATGGAATGTTCTTTAAAATTCAACACC 95238.doc -86 - 1327645 CACCAGGCTCTAAGCGGCGATCTGTGCTAAGAGGTCAGGACCCAGCCGAA GTCCTCGGCGTTGACAGGCAGCTGGGGGGACATGATCCATGGACAAGGCC ATCCCGGCCGTGGGAGACCCCAGTCCCGAAGTCTTGCCTGCAGGAGTACT GGGGTCCCCCTGGGGCCCTCTTTACTGTCACGTCATCTCTAGGAAACCTAT CTCTGAGTTTTGGGACCAGGTCGGTTTGGGTTTGAATTCTGCCTCTTCTTGC TCACTGTGTGACCAAGTGACAAACTCCTTCTGAACCTGTGTTCTCCCACTG TACCAGGGCTGTTCTGTGGTCCCCGTGAGTGCCAAGCATACAGTAGGGGC TCAATAAATCCTTGT Sequence No. 1 〇7 immunohistochemical tissue blocks were cut from the frozen sections at 8 uM, APES slide and placed on a "dry before, in acetone Fix the tissue for 10 minutes. The slides were then immersed in 0.3% hydrogen peroxide in methanol for 1 minute and rinsed with scaly-buffered saline (PBS). The non-specific binding sites were blocked by culturing the slides in 20% serum from appropriate animals and flushing again with PBS. Primary antibodies diluted in PBS containing 1% serum were then added to the slides. After 1 hour of incubation, the slides were again washed with PBS and then incubated with secondary antibodies for 1 hour. After the final rinse with PBS, the secondary antibody was detected using diaminobenzidine tetrahydrochloride dissolved in Tris buffered saline (TBS) and then rinsed with TBS and water. The slides were then stained with hematoxylin and examined under an optical microscope. In certain embodiments, gastric tumors can be localized in situ using staining based on the cancer marker of the present invention. At least one marker can form a powder-like protein structure that can be stained using Congo red or equivalent non-specific amyloid protein staining. Testing gastric cancer markers in body fluids in several specific embodiments, possibly Want to obtain samples from blood, plasma, serum, using peritoneal fluid obtained by, for example, peritoneal washing, or other body, such as urine, lymph, cerebrospinal fluid, gastric juice, or stool sample, 95238.doc • 87-1327645 , GTM determination. In general, methods for determining oligonucleotides, proteins and peptides in these fluids are known in the art. Detection of oligonucleotides can be carried out using hybridization methods such as Northern blotting, Southern dot method, or microarray or qPCR. Methods for detecting proteins include, for example, enzyme-linked immunosorbent assay (ELISA), antibody-containing protein wafers, suspension bead radioimmunoassay (RIA), Western 'ink dot method, and lectin binding. However, for understanding, the Sanming-type-type enzyme-linked immunosorbent assay (ELISA) can be used to quantify the fluid content of GTM. For plasma assays, a 5 microliter aliquot of a suitably diluted sample or serially diluted standard GTM was added to a well of a microtiter plate with 75 microliters of peroxidase-conjugated anti-human GTM antibody. After incubation at 30 °C for 30 minutes, each well was washed with 0.05% Tween 20 in phosphate buffered saline (PBS) to remove unbound antibody. The binding complex of GTM and anti-GTM antibody was then incubated with o-phenylenediamine containing H202 for 15 minutes at 30 °C. The reaction was stopped by the addition of 1 M H2S04, and the absorbance at 492 nm was measured using a microtiter plate reader. It is understood that the anti-GTM antibody may be a monoclonal antibody or a plurality of antiserum. It is also known that any other body fluids can be properly studied. Certain markers that appear in plasma or serum t are known. These include osteogenic proteins (Hotte et al., Cancer 95(3): 507-510 (2002)), prostate-specific antigens (Martin et al., Prostate Cancer Prostatic Dis. (March 9, 2004) (publication) No. PMID: 15007379), squamous gland eggs 95238.doc -88-1327645 White (Hall et al., Laryngoscope 113(1): 77-81 (2003); Mazzaferri et al., J. Clin. Endocrinol. Metab. 88 (4): 1433-14421 (2003)), matrix metalloproteinases-2 and -9 (Kuo et al, Clin. Chem. Acta. 294(1-2): 157-168 (2000)), CEA and TIMP1 ( Pellegrini et al, Cancer Immunol. Immunother. 49(7): 3 88-394 (2000). Therefore, because some of the above markers are also useful GTM markers, plasma has been available for its detection and quantification. , serum or other fluids. Because many proteins are (1) secreted by cells, (2) shed from the cell membrane, or (3) lost from the cell when the cell dies, other GTMs may also be present in body fluids, such as plasma, In serum and its analogs. Thus, in a particular embodiment of the invention, it will be useful to detect GTM in a conveniently obtained sample. Hope, and may be the basis for the diagnosis of gastric cancer. Western analysis uses TriReagent and 胍HC1 extraction method to extract proteins from gastric tissue. Mix the non-liquid phase extracted from RNA TriReagent with 1.5 volumes of ethanol, and centrifuge DNA and OCT media were removed. 0.5 ml of the supernatant was mixed with 0.75 ml of isopropanol, incubated at room temperature for 10 minutes, and then centrifuged. The pellet was washed with 1 ml of 0.3 M in 95% ethanol. 3 times and rinsed once with ethanol and then resuspended in 50 μl of 1% SDS. Protein was quantified and electrophoresed on SDS polyacrylamide gel using standard methods. Briefly, using standard methodology, using The cells were transferred by BioRad reverse-blot electrophoresis, and the separated proteins were transferred to a PVDF membrane. The membrane was then blocked with a solution containing skim milk powder for 30 minutes, followed by 95238.doc-89-1327645 primary antibody at room temperature. The culture was carried out for 2 hours. After the rinsing, the membrane was incubated with the secondary antibody for hrs at room temperature. After the final rinsing, the antibody was bound using the ECL detection system (Amersham out (4)^(8)). Color development...by providing a serum sample by using a known method, and then: subjecting the serum sample to the X analysis U directly against the oligonucleotide of the egg of interest or the antibody, to complete the marker in the serum Detection. The immune dot method, including Western blot analysis, may be particularly useful for determining whether a protein that is otherwise expressed is present in serum (4). In addition, other body fluids may also contain markers, including peritoneal fluid, cerebrospinal fluid, and the like. For secreted markers, it does not have to be useful in physiological terms. Rather, any mechanism by which a marker protein or gene enters the serum may be effective in producing a detectable, quantifiable marker. Thus, a sufficient amount of useful markers can be produced by the normal secretion of soluble proteins from the cells, the detachment of membrane proteins from the plasma membrane, the secretion of alternative forms of mRNA or proteins expressed therefrom, and cell death (cell dying). There is increasing support for the use of serum markers as a tool for diagnosing and/or assessing the efficacy of treatment for various cancer types.

Yoshikawa et al. (Cancer Letters, 151:81-86 (2000)) describe a tissue inhibitor of matrix metalloproteinase_丨 in a mortar of a patient with gastric cancer.

Rudland (Cancer Research 62: 3417-3427 (2002)) describes osteogenic proteins as proteins involved in metastasis in human breast cancer.

Buckhaults et al. (Cancer Research 61: 6996-7001 (2002)) describe 95238. doc-90- 1327645 certain secretory and cell surface genes that are expressed in colorectal tumors.

Kim et al. (JAMA 287(13): 1671-1679 (2002)) describe osteogenic proteins as potential diagnostic biomarkers for ovarian cancer.

Hotte et al. (AJ. American Cancer Society 95(3): 507-512 (2002)) describe plasma osteogenic proteins as proteins detectable in human body fluids and are associated with certain malignancies.

Martin et al. (Prostate Cancer Prostatic Dis. March 9, 2004 (PMID: 15007379) (abstract)) describes the use of human kallikrein 2, prostate-specific antigen (PSA), and free PSA for detection of prostate cancer. Marker.

Hall et al. (Laryngoscope 113(1): 77-81 (2003) (PMID: 12679418) (abstract)) describe the predictive value of serum thyroglobulin in thyroid cancer.

Mazzaferri et al. (J. Clin. Endocrinol. Metab. 88(4):1433-144 1 (2003) (abstract)) describe thyroglobulin as a possible monitoring method for patients with thyroid cancer.

Whitley et al. (Clin. Lab. Med. 24(1): 29-47 (2004) (abstract)) describes thyroglobulin as a serum marker for thyroid cancer.

Kuo et al. (Clin. Chim. Acta. 294(1-2): 157-168 (2000) (summary)) describe serum matrix metalloproteinases-2 and -9 in patients infected with HCF and HBV.

Koopman et al. (Cancer Epidemiol. Biomarkers Prev 13(3): 487-491 (2004) (abstract)) describes osteogenic protein as a biomarker for pancreatic cancer.

Pellegrini et al. (Cancer Immunol, Immunother. 49(7): 388-95238.doc -91 - 1327645 394 (2000) (abstract)) describes the measurement of soluble carcinoembryonic antigen *τΐΜρι as an in situ colorectal cancer marker Things. Therefore, we have identified a number of genes and/or proteins that can be used to develop reagents, devices and kits for the detection and assessment of gastric cancer. One or more markers of the stomach can be used alone or in combination to provide reliable molecular testing of gastric cancer. EXAMPLES The examples described herein are merely illustrative of specific embodiments of the invention. Other specific embodiments, methods, and types of analysis are within the skill of those skilled in the art of molecular diagnostics' need not be described in detail herein. Other specific embodiments within the scope of this technology are considered as part of the present invention. Example 1: Marker for confirming gastric malignancy Fig. 2 shows a table showing the results of research and development using 38 kinds of gastric malignant markers selected by the above criteria. Figure 2 includes the gene's symbol ("symbol"), MWG oligo number, NCBI mRNA reference sequence number, protein reference sequence number, fold change between tumor and non-tumor gene expression, relative to microarray analysis The fold change level of other genes, the results of the original unadjusted Student st. test, the Bonferroni-adjusted p-value, and the 2-sample Wilc〇x〇n test results. The fold change in the 34 genes (tumor: non-malignant tissue) ranged from 1.6 to 7 ' and the intermediate variation in the fold change range ranged from -16,995 to -25,783. The largest possible change in the fold change level is _29,718. For each of the markers that are not found, they are found to be the special markers of cancer markers, and the first ten are extremely high. The adjusted p-values are less than 10 or lower. The diagnosis indicating the use of these markers is highly correlated with the stomach 95238.doc -92· 1327645. Two cysteine protease inhibitors (CST1, CST2 and CST sentences are highly homologous and unless otherwise stated, The array is represented by the same oligonucleotide and collectively referred to as "CST1,2,4, using the SMART suite of software (Eur〇pean JWcneeuiai Bi()lc) gy Lab〇 Rat〇ry))' It is predicted that all of the proteins described in Figure 2 have signal peptides. Signal peptides are known to target synthetic proteins to the extracellular compartment and thus can be secreted into the interstitial fluid, where they can be accessed Blood. In fact, some of the proteins of the invention have been detected in serum. - Each of the genes described in Figure 2 shows more than two oligonucleotides in intensity scale on an array consistent with CEA. Changes, CEA is monitoring gastric cancer The most commonly used markers for clinical practice. Example 2: qPCR analysis Using qPCR, a subset of the genes presented in Figure 3 was obtained, and the gene expression was more sensitive and more accurate, as determined by microarray analysis. Twenty-three genes (Fig. 2) were analyzed from 46 tumors and one non-malignant sample, and the results are shown in Figure 3. Figure 3 includes the gene symbol, the intermediate fold change between cancer and normal tissue, and Tumor samples with a degree of performance exceeding 95% of the performance in non-malignant samples. 12 tumor samples and 9 normal samples were excluded from the analysis because high (>75%) normal Cell contamination, high degree of necrosis (>40%), or poor hybridization signals on microarrays. Intermediate fold changes in these 23 genes (tumor tissue compared to intermediate non-library, tissue performance), range It is from 3 to 525 times (Fig. 3) 95238.doc -93- 1327645 The degree of performance of genes ASPN, CST1, 2, 4, L0XL2, TIMP1, SPP1, SFRP4, INHBA, THBS2 and SPARC, with a case of g 9〇% 'In tumors, it is greater than 95% of the non-malignant range (Figure 3). The remaining genes, in >50% of the samples, performed more than 95% in the tumor. Each tumor overexpressed at least 7 genes over 95%, indicating that the combination of markers would guide all stomach tumors. Extensive range. Example 3: Confirmation of array data using qPCR

Array data was confirmed using a 24, gene probe on tumor and non-malignant samples using fixed-point, real-time PCR (qPCR). Of the 24 genes studied, 20 showed a strong correlation between the two technologies. Four of these analytes are shown in Figures 4a-4d, which are described using arrays and

The qPCR method detects a graph of the relative performance of the four selected cancer markers. Regarding each of the graphs in Fig. 4, the horizontal axis represents the fold change of the array l〇g2 in the gene expression, and the vertical axis represents the qpcR in the gene expression.

L〇g2 multiple change. We found a strong correlation between the two methods to represent the covariant relationship between the two methods. Strong correlations represent microarray fold change analysis and qPCR are appropriate methods for detecting changes in the expression of gastric cancer marker genes and can therefore be used as accurate and sensitive methods for selection. It can also be seen from Figures 4a-4d that qPCR can be more sensitive than array methods in detecting changes in performance. For this reason, qPCR may be particularly useful where it is particularly desirable to have an early detection. Figures 5a-5w depict comparative observations—the frequency of each of the 23 genes in the series (vertical axis) and the histogram of the 〇2 fold change (horizontal axis) in the gene representation 'including normal tissue (open bars) and Tumor tissue (black column). We found that each of these 23 genes, which are fairly separated in frequency distribution between normal and tumor tissue, reflects a low degree of overlap between the frequency distribution curves. For example, Figure 5b depicts the results for CST 1, 2, 4, where only one normal sample was observed to have a degree of performance in the tumor range. In other cases (eg, Figure 5n; for PRS11), each frequency distribution curve is relatively narrow and has some degree of overlap. However, even if the marker is in the middle of the change, the amount of gene expression is shown to be quite separate. In other cases (eg, Figure 5a; ASPN), although there is some overlap', between the normal and tumor samples, the intermediate 1 〇g2 fold performance is clearly separated. Figure 6 is a graph showing the number of genes (vertical axis) showing a marked increase in the expression ("excessive expression") in the tumor sample compared to the normal sample, and the histogram of the individual samples tested. In each case, tumor samples showed an increased degree of expression of multiple genes. The minimum number of genes with increased performance was 7, found in sample E123. These findings indicate that in the case where multiple genes are overexpressed relative to normal tissues, the reliability of cancer detection may be extremely high, making the diagnosis of cancer clearer. However, in some cases, an increase in the performance of a single marker gene is sufficient to guide the diagnosis of cancer.

We first looked at the serum markers that are most commonly used to detect gastric cancer. The CEA ratio is based on the difference in the intensity level of the array data between the tumor and the normal sample, using the qPCR data from the marker and CEA. The comparison. Figures 7a-7c depict the relative log2 expression of the marker (compared to reference RNA preparation) in 95238.doc 1327645 individual tumor samples and non-malignant samples compared to the performance of the tumor marker CEA gene. . CEA is currently the most commonly used serum marker for monitoring the progression of gastric cancer. The zero point is defined as the intermediate standard performance of each marker. It can be seen that there is a large overlap between the expression of the CEA gene (CEACAM5) in the tumor sample and the normal sample. This overlap is significantly less than in the gastric cancer markers ASPN, CSPG2, CSTi, 2, 4, IGFBP7, INHBA, L0XL2, LUM, SFRP4, SPARC, SPP1, THBS2, TIMP1, P. Kodlik, LEPRE1 and EFEMP2. As for the other markers in Figures 7b-7c, ASAH1, SFRP2, GGH, MMP12, KLK10, TG, PRSS11, and TGFBI, overlap between tumor expression range and non-malignant tissue performance range, overlap with the above markers Larger, but still smaller than CEA, it is pointed out that all of the novel markers described herein are quantitatively superior to CEA and thus provide a more reliable diagnosis. To minimize the effects of variable tissue manipulation, tumors were calculated using qPCR data from tumors and non-malignant tissue samples derived from the same patient: normal (non-malignant) fold change. This type of pairwise analysis corrects the background extent of gene expression in different individuals and minimizes the impact of tissue manipulation on RNA quality. For example, if the excised stomach is at room temperature for 1 hour, the transcripts from normal and tumor samples will degrade to the same extent. Figure 8 summarizes the degree of T:N expression of markers identified by qPCR, but uses paired data from the same individuals (i.e., tumor and non-malignant samples). Figure 8 also includes performance data for the six genes not included in Figure 3. Additional studies 95238.doc -96· 1327645 genes were MMP2, CGRll, TGFB1, PCSK5, SERPINB5 and SERPINH1. The confirmed information and probes are shown in Figures 1 and 2. Figure 8 shows the intermediate T:N fold change and maximum T:N fold change for 29 gastric cancer markers in these 40 patients with "paired" samples. Twenty-seven of the 29 markers have a greater than or equal to the prior art identification, and the intermediate T:N difference of CEA. The 29/29 marker has a higher percentage of paired samples in which the performance in the tumor sample exceeds the performance in the normal sample. Figures 9a-9d depict dot plots of data from tumors and normal tissues from the same individual. Each point represents a fold-change in the performance of the marker of the tumor tissue relative to the performance in the non-malignant tissue in the patient. All markers for the study had better resolution of tumor and non-tumor tissue than CEA. The three markers, CST1, 2, 4, ASPN and SFRP4, show 100% resolution between pairs of tumors and normal samples, ie for each marker, each tumor tissue has a comparable The corresponding non-tumor samples from the same individuals have greater performance. In many other markers, such as Adelikan, CSPG2, EFEMP2, IGFBP7, INHBA, LOXL2, LUM, SERPINH1, SPARC, SPP1, TGFbl, THBS2, and TIMP1, all in only 2 or 3 individual sites, tumors Tissue performance is lower than non-tumor tissue. Therefore, for those markers, the probability of any pair of tumors and non-tumor tissue will produce false negatives is relatively low (eg 3 out of 40, or 7.5%; 2 out of 40) Or 5%, one of 40, or 2.5%). Therefore, even with the other markers listed immediately above, the use of multiple samples from individual patients will yield reliable diagnostic information. 95238.doc -97- 1327645 The gene sequences of these markers are shown above, as well as the positions of the primers and probes used to detect them. ^ Judging whether the overexpression of the marker gene is independent of the stage of the gastric tumor. The paired T:NI〇g2 fold change is plotted against the tumor stage (Fig. _a_ 1 Oad) versus the 26 markers listed in Figure 8. It was observed that there was no phase dependence on the tumor stage. These markers are overexpressed in a similar manner in early and late stages of the tumor. However, KLK1〇 showed a more consistent overexpression in stage 丨 and stage 2 tumors, while pcSK5 and SERPINB5 showed a more over-expressed performance in stage 4 tumors. Therefore, KLK10, PCSK5, and SERPINB5 can be used to determine the stage of gastric tumors. In a similar assay, a pairwise change in T:N 1〇g2 is plotted against the LaUren classification (diffuse or intestinal) of the tumor. Figure 11a_llad shows that the tumor type is intestinal (1) or diffuse (D), and 29 gtMs can distinguish between tumor and non-tumor tissue. Example 4: Use of Multiple Markers As noted above, certain markers showed the ability to distinguish between tumors and non-tumor tissue in samples of i 〇〇 %. Other markers, also described above, can be used in combination to achieve a very high degree of discrimination between tumors and non-tumor tissue. Figure 12 depicts a 3-dimensional plot of the three markers SERPINH1, CST1, 2, 4 and inhba, expressed as a log2 T:N fold change for a series of gastric tumor samples and non-malignant stomach samples. There is complete separation between the two sets of samples. The statistical analysis outlined in Figure 13 is used to further explain the use of the marker 95238.doc -98. 1327645, and s 'continuously distinguishes between tumor and non-tumor. This analysis uses the standard distribution of the data generated by the paired tumors and the qpcR gene expression of the paired tumor and the Asian-style model, showing that the wood is used to distinguish between tumors and non-malignant samples. The effect of the number on test sensitivity (with 95% fixed specificity). Although a few of the 29 markers (as shown in Figure 8) 'when alone in this analysis' have a super-sense, 95 or 99% sensitivity but a combination of two or three markers 'make large The combination of numbers can achieve high sensitivity

For example, a combination of 50 two markers will be g 99. /. Sensitivity and $95% specificity identify tumor and non-tumor samples. Example 5: Detection of gastric tumor marker proteins

In another implementation, T detects dome proteins as a basis for diagnosis. In some cases, the concentration of mRNA in a particular sample, such as a sample without cells, may not be easily detected using microarray or qPCR methods to detect an increase in gene expression. Thus, in certain embodiments, the detection of GTM proteins can be accomplished using antibodies directed against the entire protein, protein fragment (peptide) or protein core. Methods for detecting and quantifying the expression of proteins and peptides are known in the art and can include methods that rely on specific antibodies to the protein or peptide. Early strain antibodies and multiple strains of antiserum can be made using methods well known in the art and need not be further described herein. To demonstrate that GTM proteins can be used to distinguish between tumor and non-tumor tissue, obtain anti-SPARC (R&D Systems; catalogue AF941), THBS2 (Santa Cruz Biotechnology Inc; catalogue sc-7655),

CSPG2 (Calbiochem; Catalog No. 428060) and IGFBP7 (R&D 95238.doc -99-1327645

Commercially available antibodies of Systems; Catalog No. AF1334). An additional polyclonal antibody against the cysteine protease SN peptide sequence 50-66(C) FAISEYNKATKDDYYRR, SEQ ID NO: 108 can be raised in rabbits (Alpha Diagnostic International Inc; San Antonio). These antibodies are used in immunohistochemistry or Western analysis of tumors and non-malignant gastric tissues. These markers all showed strong tumors at the protein level: normal differences. This confirms that excessive expression of these genes is observed at the RNA level and can also occur at the protein level. φ Figure 14 shows Western blot analysis of total protein extracted from two pairs of tumor and non-malignant tissue using antibodies against proteins encoded by SPARC, CST1 (cysteine protease inhibitor SN), IGFBP7 and THBS2. For each marker, the signal in the tumor sample was significantly higher than in the non-malignant sample. The antibodies raised by the cysteine protease inhibitor SN detected three major bands, which were consistent with molecular weights of approximately 34, 45 and 65 kDa, respectively. The lowest molecular weight band is shown in Figure 14. The protein species is larger than the control cysteine protease inhibitor SN protein, suggesting that the protein is produced by tumors that have undergone post-translational modification or multimerization. Regardless of the mechanism responsible for the difference in the molecular weight of the CST protein, Figure 14 demonstrates that the CST performance is low in non-tumor tissues, but is readily observed on the tumor-derived protein dots. Figure 14 also shows that the SPARC protein is substantially expressed in tumor tissue to a greater extent than non-tumor tissue. The SPARC protein has a slower gel mobility than that detected by the protein in the serum (Figure 15), and 95238.doc -100-1327645 shows different translations in proteins produced by malignant gastric cells. modify. Irrespective of the mechanism responsible for any such modification, it was found that SPARC is overexpressed in tumor tissue relative to non-malignant tissue, representing SPArc as a useful protein marker. Similarly, IGFBP7 and THBS2 also show excessive expression in tumor tissues relative to non-malignant tissues. Immunohistochemical analysis of tumors and non-malignant tissues can be performed using antibodies against proteins encoded by CSP (32 (Foscene) and CST1 (cysteine protease inhibitor SN). Tissues against antibodies against Fossil Immunochemical analysis of immune textures confirmed strong staining in the extracellular matrix of tumor tissues, but not non-malignant tissues. Using anti-cysteine protease inhibitor SN antibody, strong observation was observed around the outer side of tumor cells. Staining. In non-malignant cells, staining with this antibody is weak and is only observed on the mucosal surface of the tissue and the lining of the gastric pit. This confirms the 'direction of the cysteine protease inhibitor in non-malignant cells. The SN protein does not enter the extracellular space on the extracellular mucosal surface. Therefore, 'not only the cysteine protease inhibitor SN protein is produced in tumor tissues in a higher amount than non-malignant tissue' and unlike The protein produced by non-malignant tissue, the tumor cysteine protease inhibitor SN is directly in contact with the tissue vasculature. To expand these observations, use monoclonal antibodies (R&D Systems; Catalogue No. AB1285), immunoprecipitation of the cysteine protease inhibitor SN from the supernatant of the gastric cancer cell line AGS (Fig. 16). A large amount of caspase was detected in the supernatant. The amino acid protease inhibitor SN, which is produced by gastric epithelial cells and secreted from the protein. Example 6: Analysis of tumor markers in serum 95238.doc • 101 - 1327645 As for markers that can be used for rapid screening 'hopefully The substance is present in the serum in an amount sufficient to detect. Certain proteins described in Figure 8 can be secreted from the gastric cancer into the blood to a detectable extent. One is known to be detectable to the extent from the stomach tumor. The marker secreted into the blood is TIMP 1. However, if a protein is secreted or detached from any cell surface other than the mucosal surface, it will come into contact with the interstitial fluid, where it may pass through the capillaries or via the lymphatic system. Direct access to the blood supply. Therefore, 'any GTM that is shed will be present in the blood. Eosin, thyroglobulin' and MMP and kinin release have been previously described. Members of the enzyme family are elevated in the serum of patients with epithelial cancer rather than gastric cancer. However, TIMP1 has previously been observed to increase in the serum of gastric cancer patients. These findings suggest a selection criterion in this study, ie Tumor tissue, but not excessive expression of secreted proteins in non-malignant tissues, can be effectively used to detect markers in serum' and thus may have substantial clinical utility without the need for tissue or organ biopsy. It is clear that serum SPARC has a different molecular weight (described herein in the Western blot method), in which the tumor SPARC has a lower molecular weight than the SPARC produced by blood cells. Therefore, even SPARC produced by tumor and non-tumor blood cells, The presence of tumor SPARC can also be determined using molecular sizes as determined using Western analysis or by antibodies specific for glycosylated proteins produced by tumor cells. In other studies, we tested the cysteine protease inhibitor SN in the supernatant of the gastric cancer cell line AGS. Figure 16 depicts Western analysis of media alone or supernatant from cultured AGS cells. The band on the right side of Figure 16 runs 95238.doc • 102· 1327645 The channel is not densely bounded by the cysteine inhibitor SN. Therefore, we conclude from Figure 1 that the GTM of the present invention is suitable for diagnosing gastric cancer in the early, middle or late stages of the disease. While certain marker proteins can be glycosylated, in some cases, changes in glycosylation patterns can result in error-detection in the form of GiMs lacking the normal glycosylation pattern. Thus, in certain embodiments of the invention, the GTM immunogen may comprise a demethylated GTM or a deglycosylated GTM fragment. Deglycosylation can be accomplished using one or more glycosylases known in the art. Alternatively, GTM CDNA, such as prokaryotic cell lines, including E. coli, can be expressed in a glycosylation-deficient cell line, thereby producing a non-glycosylated protein or peptide. It is also known that the redness and quality of glycosylation may be sensitive to the presence of the necessary precursors of the sugar side chain. Therefore, in the absence of the necessary sugar, "normal" glycosylation may not occur, but shorter or missing side chain sugars may be formed. Such "glycosylation variants" can be used as immunogens to generate antibodies of different types of marker genes. In addition, certain GTMs can form homologous or heterodimers, or other types of Poly form. For example, inhibin j3A is a 47 kDa protein that forms a 97 kDa molecular weight homodimer (activin A) and a 92 kDa heterodimer with a 45 kDa protein inhibitory cation (the heterogeneous The polymer is called activin AB). Therefore, it is possible to understand Western analysis or other types of measurements that do not have to be limited to the detected monomeric form. Rather, it is quick to know that any form of gtm can be detected, regardless of molecular weight. Therefore, the presence of gastric cancer can be easily diagnosed using the GTM test of the polyform form 95238.doc -103-1327645. In addition, for gTm that is selective for stage (1-4) or type (diffuse or intestinal) of gastric tumors, detection of the multimeric form provides an appropriate standard for assessing the stage or type of gastric cancer. Such antibody preparations can be used in a variety of ways to produce antibodies or antisera against GTM. First, the GTM protein or form can be quantified using an enzyme-linked immunosorbent assay (ELISA) or a radioimmunoassay (RIA) method. Immunohistochemistry can be performed in tissues using immunohistochemistry. These methods are known in the art and need not be further described herein. Example 7: Vectors Containing GTM Oligonucleotides Other specific embodiments of the invention include vectors that can be used in vitro to express fragments of a marker gene or a protein thereof ("marker peptide,") or a marker gene product. For example, vectors having oligonucleotides encoding GTMs can be made. Many such vectors can be based on standard vectors known in the art. The invention also encompasses cells that can be used to transfer infection to various cell lines to produce GTM-producing cells. The vector of the strain, which can be used to produce the desired amount of gTMs, in order to develop specific antibodies or other reagents for detecting GTMs, or to measure the development of GTMs by standardization. It should be understood that the manufacture of such vectors will contain expressions to be expressed. a plasmid inserted into a vector, or a portion of such an open-architecture, into a vector containing a promoter gene region, one or more promoter regions, which are operable In a manner that is linked to the nucleotide sequence, as well as the initiation codon 'open-editing framework and stop codon. The method of generating the expression vector is It is understood that it is not necessary to repeat herein. It is also understood that one or more selectable markers can be inserted into the expression vector, 95238.doc.104. 1327645 allows for the selection of a cell line containing the expression vector of interest. A leader sequence known in the art can also be inserted into the framework to direct secretion, internal storage or insertion into the membrane of the protein or protein fragment in the expression cell. Example 8: Transfer of infected cells with a GTM-containing vector In another specific embodiment, cells are provided that can express GTMs, GTM fragments or peptide markers. Prokaryotic and eukaryotic cells can be used. For example, E. coli (prokaryotic cells) can be used to produce large amounts of GTMs, which are lacking Mature glycosylation (if a particular GTM is normally glycosylated). COS cells, 293 cells, and various other eukaryotic cells can be used to produce glycosylated GTMs' or have appropriate folding, And thus the two-dimensional structure of the native form of the GTM protein. Methods for transferring infections of such cells are known in the art and need not be included herein. Example 9 Step Description: Pack

Based on the findings of the present invention, several types of test kits can be produced. First, a kit having a detection device preloaded with a detection molecule (or "capture reagent,)) can be manufactured. In a specific embodiment for detecting GTM mRNA, such devices may include a substrate (eg, glass, ruthenium, quartz, metal, etc.) having a nucleus on it that acts as a capture reagent and hybridizes to the mRNA to be detected. Glycoside. In some embodiments, direct detection of mRNA can be accomplished by tying the mRNA (indicated by Cy3, ey5, radiolabel or other marker) to the oligonucleotide parent on the substrate. In other specific embodiments, the complementary cDNA (cDNA) of the desired mRNA can be first produced to complete the detection of the mRNA, and the labeled cDNA can be hybridized with the oligonuclear 95238.doc 1327645 acid on the substrate. And detect it. Regardless of the method of detection used, it is desirable to compare the standard measurements of the performance and performance of the tested GTM. For example, the total cell can, the RNAs that represent the composition (eg, ribosomal RNA), or other relevant invariant markers The substance is standardized for RNA expression. Antibodies can also be used as capture reagents in kits. In some embodiments, a substrate (e.g., a multi-well plate) can have a specific gtm capture reagent attached thereto. In some embodiments, the kit can have a blocking reagent. Blocking agents can be used to reduce non-specific binding. For example, non-specific oligonucleotide binding can be reduced using excess DNA derived from any convenient source that does not contain GTM oligonucleotides, such as salmon sperm DNA. Excessive blocking proteins, such as serum albumin, can be used to reduce non-specific antibody binding. A number of methods are known for detecting oligonucleotides and proteins, and any strategy for specifically detecting GTM-binding molecules can be used and is considered to be within the scope of the invention. In specific embodiments that rely on antibody detection, the gtm protein or peptide can be expressed on a per cell basis, or on a total cell, tissue or fluid protein, fluid volume, tissue mass (by weight). In addition, GTM in serum can be expressed relative to a relatively high serum protein, such as albumin. In addition to the text, the test kit can include capture reagents (such as probes), flushing baths (such as SSC 'other salts, buffer solutions, detergents, and the like) and detection sections (such as Cy3, cy5). , radioactive markers and their analogues). The kit can also include instructions for use and packaging. Although the present invention is described with reference to specific specific embodiments thereof, it is understood that other specific embodiments are directed to the use of the disclosed identifiers, without departing from the scope of the invention. Industrial Applicability Methods for detecting GTM family members' include detection of nucleic acids using microarrays and/or real-time PCR, and detection of proteins and peptides. The compositions and methods of the present invention can be used to make diagnostic devices and kits, to diagnose disease, to evaluate the efficacy of treatment, and to produce reagents suitable for measuring the performance of GTM family members in biological sample clocks. BRIEF DESCRIPTION OF THE DRAWINGS The invention will be described with reference to the specific embodiments thereof and with reference to the accompanying drawings, in which: FIG. 1 illustrates the oligonucleotide sequence of the gastric cancer marker and marker of the present invention. The microarray method was used to perform a table of the results obtained by the study. Figure 3 depicts the results obtained using the quantitative PCR for the study. Figure 4a-4d illustrates the relationship between the results of the i〇g2 multiple obtained using the microarray and the qpCR method, where the data is concentrated in four gastric cancer markers. The intermediate normal state of matter. Gray squares correspond to non-malignant ("normal") specimens, while black triangles are tumor specimens. Figure 4a: ASpN. Figure 4b: sppi. Figure 4c: SPARC Figure 4d: MMP12. Figure 5a-5w The relative frequency is a histogram of the log2 multiple of the quantitative PCR studies obtained from various tumor markers. The histogram of the data is shown in Fig. 5a: ASPN; Fig. 5b. CST1, 2&4; Fig. 5c · r Qdr. - m) e. CSPG2; Fig. 5d: IGFBP7; Fig. 95238.doc -107·1327645 5e: INHBA; Fig. 5f: L0XL2; Fig. 5g: LUM; Fig. 5h: SFRP4; Fig. 5i: SPARC; Fig. 5j: SPP1 Figure 5k: THBS2; Figure 51: TIMP1; Figure 5m: Adlican; Figure 5n: PRS11; Figure 5o: ASAH1; Figure 5p: SFRP2; Figure 5q: GGH; Figure 5r: MMP12; Figure 5s: KLK10; Figure 5t · LEPRE1; Figure 5u: TG; Figure 5v: EFEMP2 and Figure 5w: TGFBI Figure 6 is a histogram showing the number of markers with a higher performance than 95% of the normal performance. Based on the data, and showing the results of each tumor sample separately. Figures 7a-7c are shown showing the gene expression of the tumor marker CEA, A graph of the relative log2 performance of markers in individual tumor samples and non-malignant samples. CE A is the most commonly used serum marker for monitoring gastric cancer. Figure 8 shows a table supplementing Figure 3. Figure 8 is summarized by qPCR The degree of performance of the candidate tumor markers was determined, but the paired data (i.e., tumors from the same individual ("T") and non-tumor ("N") samples were used to provide a T:N ratio. Figure 8 also includes markers other than Figure 3, namely MMP2, CGR11, TGFB1, PCSK5, SERPIMB5, SERPINH1. For comparison, the degree of expression of the established serum marker gene CEACAM5 (CEA) is also shown. 27 intermediate T:N differences greater than or equal to CEA. Furthermore, compared to CEA, the 29/29 marker has a higher percentage of paired samples, where the performance in the tumor sample exceeds the normal sample The performance of the three markers, CST1, 2, 44, ASPN and SFRP4, showed a 100% difference between the paired tumor and the normal sample. The gene sequences of these markers are shown in this document, as well as the primer and probe positions to detect them. Figures 9a-9d depict individual and intermediate T:N fold change data for 29 gastric cancer markers using paired samples in 40 patients.

Figures 10a-10ad illustrate graphical representations of tumor stage and log2 fold changes in the performance of CEA and other GTMs of the invention. Figure l〇a: Adelicon; Figure 10b: ASPN; Figure 10c: CSPG2; Figure 10d: CST1, 2, 4; Figure 10e: EFEMP2; Figure 10f: GGF; Figure 10g: INHBA; Figure 10h: IGFBP7; 10i: KLK10; Fig. 10j: LEPRE1; Fig. 10k: LUM; Fig. 101: LOXL2; Fig. 10m: MMP12; Fig. 10n: TIMP1; Fig. 10〇: ASAH1; Fig. 10p: SPP1; Fig. 10q: SFRP2; Fig. l〇r: SFRP4; Figure 10s: SPARC; Figure 10t:

Figure 10u: THBS2; Figure 10v: TG; Figure 10w: TGFBI; Figure 10x: CGR11; Figure 10y: SERPINH1; Figure 10z: MMP2; Figure 10aa: PCSK5; Figure 10ab: SERPINB5; Figure l〇ac: TGFB1 and Figure 10ad : CEA (CEACAM5). Figures 11a-llad illustrate graphical representations of tumor type (diffuse (D) or intestinal (I)) and l〇g2 fold changes in the presentation of the 29 GTM and CEA of the present invention. Figure 11a: Adelicon; Figure lib: ASPN; Figure 11c: CSPG2; Figure lid: CST1, 2, 4; Figure lie: EFEMP2; Figure Ilf: GGH; Figure llg: INHBA; Figure 11h: IGFBP7; KLK10; Fig. 11j: LEPRE1; Fig. Ilk: LUM; Fig. 111: LOXL2; Fig. 11m: MMP12; Fig. 11n: TIMP1; Fig. 11〇: ASAH1; Fig. Lip: SPP1; Fig. 11q: SFRP2; Fig. 11r: SFRP4; : SPARC ; 95238.doc •109· 1327645 Figure lit : PRSSll ; Figure 11u : THBS2 ; Figure 11v : TG ; Figure 11w : TGFBI ; Figure llx : CGR11 ; Figure lly : SERPINH1 ; Figure llz : MMP2 ; Figure 11aa : PCSK5 ; Figure 11ab: SERPINB5; Figure 11ac: TGFB1 and Figure 11ad: CEA (CEACAM5). Figure 12 depicts a three-dimensional illustration of three markers SERPINH1, CST1, 2, 4 and INHBA in a series of gastric tumor samples and non-malignant stomach samples. Figure 13 depicts a table showing the effect of multiple markers on the ability to accurately discriminate between tumor tissue and non-malignant tissue. This form is derived from the normal distribution derived from the qPCR data. Figure 14 is a western blot of four tumor markers derived from tumor and non-tumor tissue. Figure 15 shows the western blot of the tumor marker SPARC in gastric tumor tissue and in serum. Figure 16 is an immunoblotting depicting a cysteine kinase inhibitor (cystatin) SN in the supernatant of the gastric cell line AGS. 95238.doc 110- 1327645 Sequence <110>New Zealand Business Pacific Aegis Technology Co., Ltd.

Guilford, Parry J.

Holyoake, Andrew J. <120> marker for detecting gastric cancer <130> PEBL-1006WO0 <140> 093124387 <141> 2004-08-13 <150> US 60/487,906 <151> 2003-07-17 <160> 108 <170> Patentln version 3.2 <210> 1 <211> 26 <212> DNA <213> Human <400> 1 aaatacaaaa ggacacattc aaagga 26 <210> 2 <211> 20 <212> DNA <213> Human <400> 2 gccagtggaa tgatgttccc 20 <2i0> 3 <2ll> 19 <212> DNA <213> Human <400> 3 agtcccagcc Caacttgga 19 <210> 4 <211> 17 <212> DNA <213> Human <400> 4 gtggcaatgc cgctgaa 17 <210> 5 95238.doc 1327645 <211> 18 <212> DNA <;213>Human<400> 5 caggtcagca agggcacc <210> 6 <211> 24 <212> DNA <213> Human. <400> 6 acaacatgat atgtgctgga ctgg <210> 7 <211><212> DNA <213> Human <400> 7 cttgagtaca acgctgacct cttc <210> 8 <211> 24 <212&g t; DNA <213> Human <400> 8 gattcttgtc catagtgcat ctgc <210> 9 <211> 19 <212> DNA <213> Human <400> 9 aggccagctt ctgcttgga <210> 10 < 211 > 23 <212> DNA <213> Human <400> 10 gcctctctgc tgatgacata cgt <210> 11 95238.doc 1327645 <211> 21 <212> DNA <213> Human <400> Ccagaccacc ttataccagc g <210> 12 <211> 17 <212> DNA <213> Human <400> 12 cgcagaacgc ctgcaaa <210> 13 <211> 18 <212> DNA <213><400> 13 cgctagcagc gaccacct <210> 14 <211> 23 <212> DNA <213> Human <400> 14 tcttccctgt acactggcag ttc <210> 15 <211> 19 <212> DNA <213>Human<400> 15 tcgggaggcc cgttagtaa <210> 16 <211> 23 <212> DNA <213> Human <400> 16 tggaaggact acacggccta tag <210> 17 95238.doc 1327645 <;211> 20 <212> DNA <213>Human<400> 17 gacggtt Cct cgcagttcaa <210> 18 <211> 16 <212> DNA <213> Human <400> 18 ctgcccaccc cttcca <210> 19 <211> 21 <212> DNA <213> Human <;400> 19 tccacgcatt ttccaggata a <210> 20 <211> 22 <212>DNA <213>Human<400> 20 ggtccatgtc atcaccaatg tt <210> 21 <211> 21 <212> DNA <213> Human <400> 21 aaaaatcttt gccggaaatg c <210> 22 <211> 20 <212> DNA <213> Human <400> 22 ttgatggcat cgctcagatc <210> 23 95238.doc 1327645 <;211> 23 <212> DNA <213>Human<400> 23 tgcttctgca attctgatat gga <210> 24 <211> 23 <212> DNA <213> Human <400> 24 tcttggcatt ttctacaaca ggg <210> 25 <211> 24 <212> DNA <213> Human <400> 25 gggaacttcg tagatctgga aaga <210> 26 <211> 25 <212> DNA <213> Human <4〇0> 26

Tgacagcaac aactcagtag gaaaa <210>27 <211> 22 <212> DNA Human <400> 27 tcacagctca agtacacctg gg <210> 28 <211> 20 <212> DNA <213> Human <400>; 28 gagaggatgc cttggagggt <210> 29 95238.doc 1327645

<211> 23 <212> DNA <213> Human <400> 29 ccgtgacaca gttctgctta cag <210> 30 <211> 21 <212> DNA <213> Human <400> 30 ccaatcaatg ccaggaagag a <210> 31 <211> 17 <212> DNA <213> Human <400> 31 ccctgatcgc cgagttg <210>32 <211> 25 <212> DNA <213> Human <400> 32 agtgacagca tcaaaactca aattg <210> 33 <211> 20 <212> DNA <213> Human <400> 33 ggacctgtgg aagtatccgc <210> 34 <211> 25 <212> DNA <213>Human<400> 34 acaggacatc atacatggtt tcaaa <210> 35 95238.doc 1327645 <211> 23 <212> DNA <213> Human <400> 35 ttttgcaggc ttcacatacc ttt <210> 36 < 211 > 18 <212> DNA <213> Human <400> 36 gaaaaagcgg gtggtgca <210> 37 <211> 24 <212> DNA <213> Human <400> 37 aaggagattc cagctgtcac tttc <210> 38 <211> 28 <212> DNA <213> Human & l t;400> 38 taggtttggt catagatagg tcctgagt <210> 39 <211> 22 <212> DNA <213> Human <400> 39 tgtaaaccgc tccacttcac at <210> 40 <211> 25 <212> DNA <213> Human <400> 40 ttctgtcctt cctagtccct ttagg <210> 41 95238.doc 1327645 <211> 21 <212> DNA <213> Human <400> 41 aagccgaatt tgctagttgc a <210> 42 <211> 22 <212> DNA <213> Human <400> 42

Tctgcaagtt catcccctct tt <210> 43 <211> 21 <212> DNA <213> Human <400> 43 agtcctggcc gttgaaatac c <210> 44 <211> 19 <212> DNA <213> Human <400> 44 tgtcacgtgg cgtcacagt <210> 45 <211> 34 <212>DNA <213> Human <400> 45 ttggaaatga gtgcaaaccc tcttgataat aatg <210> 46 <211> 23 <212&gt DNA <213>Human<400> 46 aggaacagtt gcttgcggcc age <210> 47 95238.doc 1327645 <211> 29 <212> DNA <213>Human<400> 47 agccagaact gcagaagaaa cagttgtgc <210&gt 48 <211> 29 <212> DNA <213> Human <400> 48 ttcactggag gtcaattgca cagcagaat <210> 49 <211> 26 <212> DNA <213> Human <400> Agcaaggtcc ttccatagtg acgccc <210> 50 <211> 25 <212> DNA <213> Human <400> 50 cttgccagag tgactctgga ggccc <210> 51 <211> 30 <212> DNA <213> Human <400> 51 ccatcacaga tcattaca Tc caggtcctca <210> 52 <211> 36 <212> DNA <213> human <400> 52 taaggattca aaccatttgc caaaaatgag tctaag <210> 53 95238.doc 1327645 <211> 33 <212> DNA <213> Human <400> 53 cgtaattctt ctggatgtct ccttcacatt ctg <210> 54 <211> 30 <212> DNA <213> Human <400> 54 tcagtccctg tatggagacc caaaagagaa <210> 55 <211&gt 33 <212> DNA <213> Human <400> 55 caagatgacc aagatgtata aagggttcca age <210> 56 <211> 28 <212> DNA <213>Human<400> 56 tgtctgaacc gcaccagcca agagaata <;210> 57 <211> 22 <212> DNA <213> Human <400> 57 ctgccagcca ccgaggaagc tc <210> 58 <211> 22 <212> DNA <213> Human <400> 58 tggaccagca ccccattgac gg <210> 59 95238.doc 1327645 <211> 31 <212> DNA <213>Human<400> 59 agtgttaatt ccaatcactt caccgtccag g <210> 60 <211> 27 <212> DNA <213> Human <400> 60 aggcccaaga ccggctacat cagagtc <210> 61 <211> 25 <212> DNA <213>Human<400> 61 tctggcagat tccgatgccc cacaa <210> 62 <211> 20 <212&gt ; DNA <213> Human <400> 62 ccaggccagg agcagctcgg <210> 63 <211> 21 <212> DNA <213> Human <400> 63 tgactccagg cccgcaatgg a <210> 64 <211> 25 <212> DNA <213>Human<400> 64 cagcctccag ccaacagacc tcagg <210> 65 95238.doc 1327645 <211> 29 <212> DNA <213> Human <400> 65 acagaatgta Gggatgggtt aagcctgca 29 <210> 66 <211> 23 <212> DNA <213> Human <400> 66 ttcaaggacc ggttcatttg gcg 23 <210> 67 <211> 1778 <212> DNA <213>; human <400> 67 tagaagttta caatgaagtt tcttctaata ctgctcctgc aggccactgc ttctggagct 60 cttcccctga acagctctac aagcctggaa aaaaataatg tgctatttgg tgagagatac 120 ttagaaaaat tttatggcct tgagataaac aaacttccag tgacaaaaat gaaatatagt 180 ggaaacttaa tgaaggaaaa aatccaagaa atgcagcact tcttgggtct gaaagtgacc 240 gggcaactgg acacatctac cctggagatg atgcacgcac ctcgatgtgg agtccccgat 300 ctccatcatt tcagggaaat gccagggggg cccgtatgga ggaaacatta tatcacctac 360 agaatcaata attacacacc tgacatgaac cgtgaggatg ttgactacgc aatccggaaa 420 gctttccaag tatggagtaa tgttaccccc ttgaaattca gcaagattaa cacaggcatg 480 gctgacam tggtggutttgcccgtgga gctcatggag acttccatgc ttttgatggc 540 aaaggtggaa tcctagccca tgcttttgga cctggatctg gcattggagg ggatgcacat 600 ttcgatgagg acgaattctg gactacacat tcaggaggca caaacttgtt cctcactgct 660 gttcacgaga ttg £ ccattc cttaggtctt ggccattcta gtgatccaaa ggctgtaatg 720 ttccccacct acaaatatgt cgacatcaac acatttcgcc tctctgctga tgacatacgt 780 ggcattcagt ccctgtatgg agacccaaaa gagaaccaac gcttgccaaa tcctgacaat 840 tcagaaccag ctctctgtga ccccaatttg agttttgatg ctgtcactac cgtgggaaat 900 aagatctttt tcttcaaaga caggttcttc tggctgaagg tttctgagag accaaagacc 960 • 12 ·

95238.doc 1327645 agtgttaatt taatttcttc cttatggcca accttgccat ctggcattga agctgcttat 1020 gaaattgaag ccagaaatca agtttttctt tttaaagatg acaaatactg gttaattagc 1080 aatttaagac cagagccaaa ttatcccaag agcatacatt cttttggttt tcctaacttt 1140 gtgaaaaaaa ttgatgcagc tgtttttaac ccacgttttt ataggaccta cttctttgta 1200 gataaccagt attggaggta tgatgaaagg agacagatga tggaccctgg ttatcccaaa 1260 ctgattacca agaacttcca aggaatcggg cctaaaattg atgcagtctt ctattctaaa 1320 aacaaatact actatttctt ccaaggatct aaccaatttg aatatgactt cctactccaa 1380 cgtatcacca aaacactgaa aagcaatagc tggtttggtt gttagaaatg gtgtaattaa 1440 tggtttttgt tagttcactt cagcttaata agtatttatt gcatatttgc tatgtcctca 1500 gtgtaccact acttagagat atgtatcata aaaataaaat ctgtaaacca taggtaatga 1560 ttatataaaa tacataatat ttttcaattt tgaaaactct aattgtccat tcttgcttga 1620 ctctactatt aagtttgaaa atagttacct tcaaagcaag ataattctat ttgaagcatg 1680 ctctgtaagt tgcttcctaa catccttgga ctgagaaatt atacttactt ctggcataac 1740 taaaattaag tatatatatt ttggctcaaa taaaattg 1778 <210> 68 < 21 1840 < 212 > DNA < 213 > human < 400 > 68 tccacacaca caaaaaacct gcgcgtgagg ggggaggaaa agcagggcct ttaaaaaggc 60 aatcacaaca acttttgctg ccaggatgcc cttgctttgg ctgagaggat ttctgttggc 120 aagttgctgg attatagtga ggagttcccc caccccagga tccgaggggc acagcgcggc 180 ccccgactgt ccgtcctgtg cgctggccgc cctcccaaag gatgtaccca actctcagcc 240 agagatggtg gaggccgtca agaagcacat tttaaacatg ctgcacttga agaagagacc 300 cgatgtcacc cagccggtac ccaaggcggc gcttctgaac gcgatcagaa agcttcatgt 360 gggcaaagtc ggggagaacg ggtatgtgga gatagaggat gacattggaa ggag £ gcaga 420 aatgaatgaa cttatggagc agacctcgga gatcatcacg tttgccgagt caggaacagc 480 caggaagacg ctgcacttcg agatttccaa ggaaggcagt gacctgtcag tggtggagcg 540 1327645 tgcagaagtc tggctcttcc taaaagtccc caaggccaac aggaccagga ccaaagtcac 600 catccgcctc ttccagcagc agaagcaccc gcagggcagc ttggacacag gggaagaggc 660 Cgaggaagtg ggcttaaagg gggagaggag tgaactgttg ctctctgaaa aagtagtaga 720 cgctcggaag agcacctggc atgtcttccc t£tctccagc agcatccagc ggttgctgga 780 ccagggcaag agctcc ctgg acgttcggat tgcctgtgag cagtgccagg agagtggcgc 840 cagcttggtt ctcctgggca agaagaagaa gaaagaagag gagggggaag ggaaaaagaa 900 gggcggaggt gaaggtgggg caggagcaga tgaggaaaag gagcagtcgc acagaccttt 960 cctcatgctg caggcccggc agtctgaaga ccaccctcat cgccggcgtc ggcggggctt 1020 Egagtgtgat ggcaaggtca acatctgctg taagaaacag ttctttgtca gtttcaagga 1080 catcggctgg aatgactgga tcattgctcc ctctggctat catgccaact actgcgaggg 1140 tgagtgcccg agccatatag caggcacgtc cgggtcctca ctgtccttcc actcaacagt 1200 catcaaccac taccgcatgc ggggccatag cccctttgcc aacctcaaat cgtgctgtgt 1260 gcccaccaag ctgagaccca tgtccatgtt gtactatgat gatggtcaaa acatcatcaa 1320 aaaggacatt cagaacatga tcgtggagga gtgtgggtgc tcatagagtt gcccagccca 1380 gggggaaagg gagcaagagt tgtccagaga agacagtggc aaaatgaaga aatttttaag 1440 gtttctgagt taaccagaaa aatagaaatt aaaaacaaaa caaaacaaaa aaaaaaacaa 1500 aaaaaaacaa aagtaaatta aaaacaaacc tgatgaaaca gatgaaacag atgaaggaag 1560 atgtggaaat cttagcctgc cttagccagg gctcagagat gaagcagtga agagacagat 1620 tgggagggaa agggagaatg gtgt accctt tatttcttct gaaatcacac tgatgacatc 1680 agttgtttaa acggggtatt gtcctttccc cccttgaggt tcccttgtga gcttgaatca 1740 accaatctga tctgcagtag tgtggactag aacaacccaa atagcatcta gaaagccatg 1800 agtttgaaag ggcccatcac aggcactttc ctagcctaat 1840 < 210 > 69 < 211 > 2384 < 212 > DNA < 213> Human < 400 > 69 tccacacaca Caaaaaacct gcgcgtgagg ggggaggaaa agcagggcct ttaaaaaggc 60 aatcacaaca acttttgctg ccaggatgcc cttgctttgg ctgagaggat ttctgttggc 120 -14-

95238.doc 1327645 aagttgctgg attatagtga ggagttcccc caccccagga tccgaggggc acagcgcggc 180 ccccgactgt ccgtcctgtg cgctggccgc cctcccaaag gatgtaccca actctcagcc 240 agagatggtg gaggccgtca agaagcacat tttaaacatg ctgcacttga agaagagacc 300 cgatgtcacc cagccggtac ccaaggcggc gcttctgaac gcgatcagaa agcttcatgt 360 gggcaaagtc ggggagaacg ggtatgtgga gatagaggat gacattggaa ggagggcaga 420 aatgaatgaa cttatggagc agacctcgga gatcatcacg tttgccgagt caggaacagc 480 caggaagacg ctgcacttcg agatttccaa ggaaggcagt gacctgtcag tggtggagcg 540 tgcagaagtc tggctcttcc laaaagtccc caaggccaac aggaccagga ccaaagtcac 600 catccgcctc ttccagcagc agaagcaccc gcagggcagc ttggacacag gggaagaggc 660 cgaggaagtg ggcttaaagg gggagaggag tgaactgttg ctctctgaaa aagtagtaga 720 cgctcggaag agcacctggc atgtcttccc tgtctccagc agcatccagc ggttgctgga 780 ccagggcaag agctccctgg acgttcggat tgcctgtgag cagtgccagg agagtggcgc 840 cagcttggtt ctcctgggca agaagaagaa gaaagaagag gagggggaag ggaaaaagaa 900 gg £ cggaggt gaaggtgggg caggagcaga Tgaggaaaag gagcagtcgc acagaccttt 960 c ctcatgctg caggcccggc agtctgaaga ccaccctcat cgccggcgtc ggcggggctt 1020 ggagtgtgat ggcaaggtca acatctgctg taagaaacag ttctttgtca gtttcaagga 1080 catcggctgg aatgactgga tcattgctcc ctctggctat catgccaact actgcgaggg 1140 tgagtgcccg agccatatag caggcacgtc cgggtcctca ctgtccttcc actcaacagt 1200 catcaaccac taccgcatgc ggggccatag cccctttgcc aacctcaaat cgtgctgtgt 1260 gccgctgcca ccgcaccccg ccatggagcg gccgtcgctg cgcgccctgc tcctcggcgc 1320 cgctgggctg ctgctcctgc tcctgcccct ctcctcttcc tcctcttcgg acacctgcgg 1380 cccctgcgag ccggcctcct gcccgcccct gcccccgctg ggctgcctgc tgggcgagac 1440 ccgcgacgcg tgcggctgct gccctatgtg cgcccgcggc gagggcgagc cgtgcggggg 1500 tggcggcgcc ggcagggggt actgcgcgcc gggcatggag tgcgtgaaga gccgcaagag 1560 gcggaagggt aaagccgggg cagcagccgg cggtccgggt gtaagcggcg tgtgcgtgtg 1620 caagagccgc tacccggtgt gcggcagcga cggcaccacc tacccgagcg gctgccagct 1680 gcgcgccgcc agccagaggg ccgagagccg cggggagaag gccatcaccc aggtcagcaa 1740 • 15-

95238.doc 1327645 gggcacctgc gagcaaggtc cttccatagt gacgcccccc aaggacatct ggaatgtcac 1800 tggtgcccag gtgtacttga gctgtgaggt catcggaatc ccgacacctg tcctcatctg 1860 gaacaaggta aaaaggggtc actatggagt tcaaaggaca gaactcctgc ctggtgaccg 1920 ggacaacctg gccattcaga cccggggtgg cccagaaaag catgaagtaa ctggctgggt 1980 gctggtatct cctctaagta aggaagatgc tggagaatat gagtgccatg catccaattc 2040 ccaaggacag gcttcagcat cagcaaaaat tacagtggtt gatgccttac atgaaatacc 2100 agtgaaaaaa ggtgaaggtg ccgagctata aacctccaga atattatlag tctgcatggt 2160 taaaagtagt catggataac tacattacct gttcttgcct aataagtttc ttttaatcca 2220 atccactaac actttagtta tattcactgg ttttacacag agaaatacaa aataaagatc 2280 acacatcaag actatctaca aaaatttatt atatatttac agaagaaaag catgcatatc 2340 attaaacaaa taaaatactt tttatcacaa aaaaaaaaaa aaaa 2384 < 210 > 70 < 211 > 1280 < 212 > DNA < 213 > human <400> 70 tgccgcagcc cccgcccgcc cgcagagctt ttgaaaggcg gcgggaggcg gcgagcgcca 60 tggccagtcc gggctgcctg ctgtgcgtgc tgggcctgct actctgcggg gcggcgagcc 120 tcgagctgtc tagaccccac ggcgacaccg ccaagaagcc catcatcgga atattaatgc 180 aaaaatgccg taataaagtc atgaaaaact atggaagata ctatattgct gcgtcctatg 240 taaagtactt ggagtctgca ggtgcgagag ttgtaccagt aaggctggat cttacagaga 300 aagactatga aatacttttc aaatctatta atggaatcct tttccctgga ggaagtgttg 360 acctcagacg ctcagattat gctaaagtgg ccaaaatatt ttataacttg tccatacaga 420 gttttgatga tggagactat tttcctgtgt ggggcacatg ccttggattt gaagagcttt 480 cactgctgat tagtggagag tgcttattaa ctgccacaga tactgttgac gtggcaatgc 540 cgctgaactt Cactggaggt caattgcaca gcagaatgtt ccagaatttt cctactgagt 600 tgttgctgtc attagcagta gaacctctga ctgccaattt ccataagtgg agcctctccg 660 tgaagaattt tacaatgaat gaaaagttaa agaagttttt caatgtctta actacaaata 720 -16·

95238.doc 1327645 cagatggcaa gattgagttt atttcaacaa tggaaggata taagtatcca gtatatggtg 780 tccagtggca tccagagaaa gcaccttatg agtggaagaa tttggatggc atttcccatg 840 cacctaatgc tgtgaaaacc gcattttatt tagcagagtt ttttgttaat gaagctcgga 900 aaaacaacca tcattttaaa tctgaatctg aagaggagaa agcattgatt tatcagttca 960 gtccaattta tactggaaat atttcttcat ttcagcaatg ttacatattt gattgaaagt 1020 cttcaatttg ttaacagagc aaatttgaat aattccatga ttaaact £ tt agaataactt 1080 gctactcaig gcaagattag gaagtcacag aticttttci ataatgtgcc tggctctgat 1140 tcttcattat gtatgtgact atttatataa cattagataa ttaaatagtg agacataaat 1200 agagtgcttt ttcatggaaa agccttctta tatctgaaga ttgaaaaata aatttactga 1260 aatacaaaaa aaaaaaaaaa 1280 < 210 > 71 < 211 > 2993 < 212 > DNA < 213 > human < 400 > 71 ggtggcgggt ggctggcggt tccgttaggt Ctgagggagc gatggcggta cgcgcgttga 60 agctgctgac cacactgctg gctgtcgtgg ccgctgcctc ccaagccgag gtcgagtccg 120 aggcaggatg gggcatggtg acgcctgatc tgctcttcgc cgaggggacc gcagcctacg 180 cgcgcgggga ctggcccggg gtggtcc tga gcatggaacg ggcgctgcgc tcccgggcag 240 ccctccgcgc ccttcgcctg cgctgccgca cccagtgtgc cgccgacttc ccgtgggagc 300 tggaccccga ctggtccccc agcccggccc aggcctcggg cgccgccgcc ctgcgcgacc 360 tgagcttctt cgggggcctt ctgcgtcgcg ctgcctgcct gcgccgctgc ctcgggccgc 420 cggccgccca ctcgctcagc gaagagatgg agctggagtt ccgcaagcgg agcccctaca 480 actacctgca ggtcgcctac ttcaagatca acaagttgga gaaagctgtt gctgcagcac 540 acaccttctt cgtgggcaat cctgagcaca tggaaatgca gcagaaccta gactattacc 600 aaaccatgtc tggagtgaag gaggccgact Tcaaggatct tgagactcaa ccccatatgc 660 aagaatttcg actgggagtg cgactctact cagaggaaca gccacaggaa gctgtgcccc 720 acctagaggc ggcgctgcaa gaatactttg tggcctatga ggagtgccgt gccctctgcg 780 aagggcccta tgactacgat ggctacaact accttgagta caacgctgac ctcttccagg 840 -17-

95238.doc 1327645 ccatcacaga tcattacatc caggtcctca actgtaagca gaactgtgtc acggagcttg 900 cttcccaccc aagtcgagag aagccctttg aagacttcct cccatcgcat tataattatc 960 tgcagtttgc ctactataac attgggaatt atacacaggc tgttgaatgt gccaagacct 1020 atcttctctt cttccccaat gacgaggtga tgaaccaaaa tttggcctat tatgcagcta 1080 tgcttggaga agaacacacc agatccatcg gcccccgtga gagtgccaag gagtaccgac 1140 agcgaagcct actggaaaaa gaactgcttt tcttcgctta tgatgttttt ggaattccct 1200 ttgtggatcc ggattcatgg actccaggag aagtgattcc caagagattg caagagaaac 1260 agaagtcaga acgggaaaca gccgtacgca tctcccagga gattgggaac cttatgaagg 1320 aaatcgagac ccttgtggaa gagaagacca aggagtcact ggatgtgagc agactgaccc 1380 gggaaggtgg ccccctgctg tatgaaggca tcagtctcac catgaactcc aaactcctga 1440 atggttccca gcgggtggtg atggacggcg taatctctga ccacgagtgt caggagctgc 1500 agagactgac caatgiggca gcaacctcag gagatggcta ccggggtcag acctccccac 1560 atactcccaa tgaaaagttc tatggtgtca ctgtcttcaa agccctcaag ctggggcaag 1620 aaggcaaagt tcctctgcag agtgcccacc tgtactacaa cgtgacggag Aaggtg cggc 1680 gcatcatgga gtcctacttc cgcctggata cgcccctcta cttttcctac tctcatctgg 1740 tgtgccgcac tgccatcgaa gaggtccagg cagagaggaa ggatgatagt catccagtcc 1800 acgtggacaa ctgcatcctg aatgccgaga ccctcgtgtg tgtcaaagag cccccagcct 1860 acaccttccg cgactacagc gccatccttt acctaaatgg ggacttcgat ggcggaaact 1920 tttatttcac tgaactggat gccaagaccg tgacggcaga ggtgcagcct cagtgtggaa 1980 gagccgtggg attctcttca ggcactgaaa acccacatgg agtgaaggct gtcaccaggg 2040 ggcagcgctg tgccatcgcc ctgtggttca ccctggaccc tcgacacagc gagcgggtga 2100 gagcagctcg agcgggtgag agcagctggt gctgtggtga cccgttccca gagcgccctt 2160 ggtttgcctt tctcttcccc aaatcccatt gccagtggct gagacacgaa aggagcactt 2220 gggacaccag ctccaacgcc ctgtcattat ggtcacattg ccttgtcctc cctgggcctg 2280 ctgtgaacgg gatccaggtg gggaaagagg tcaagacagg gagcgatgct gagttcttgg 2340 ttccctcctt gggccccact tcagctgtcc ttttccagag agtaggacct gctgggaagg 2400 agatgagcct ggggccatta aggaaccttc cttgtcccct gggaagtagc agctgagaga 2460 • 18 ·

95238.doc 1327645

tagcgagtgt ctggagcgga ggcctctctg aatgggcagg ggtttgtcct tgcaggacag 2520 ggtgcaggca gatgacctgg tgaagatgct cttcagccca gaagagatgg tcctctccca 2580 ggagcagccc ctggatgccc agcagggccc ccccgaacct gcacaagagt ctctctcagg 2640 ca £ t £ aatcg aagcccaagg atgagctat £ acagcgtcca ggtcagacgg atgggtgact 2700 agacccatgg agaggaactc ttctgcactc tgagctggcc agcccctcgg ggctgcagag 2760 cagtgagcct acatctgcca ctcagccgag gggaccctgc tcacagcctt ctacatggtg 2820 ctactgctct tggagtggac atgaccagac Accgcacccc ctggatctgg ctgagggctc 2880 aggacacagg cccagccacc cccaggggcc tccacaggcc gctgcataac agcgatacag 2940 tacttaagtg tctgtgtaga caaccaaaga ataaatgatt catggttttt ttt 2993 <210> 72 <211> 736 <212> DNA <213> Human <400>

ggctctcacc ctcctctcct gcagctccag ctttgtgctc tgcctctgag gagaccatgg 60 cccggcctct gtgtaccctg ctactcctga tggctaccct ggctggggct ctggcctcga 120 gctccaagga ggagaatagg ataatcccag gtggcatcta tgatgcagac ctcaatgatg 180 agtgggtaca gcgtgccctt cacttcgcca tcagcgagta caacaaggcc accgaagatg 240 agtactacag acgcccgctg caggtgctgc gagccaggga gcagaccttt gggggggtga 300 attacttctt cgacgtagag gtgggccgca ccatatgtac caagtcccag cccaacttgg 360 acacctgtgc cttccatgaa cagccagaac tgcagaagaa acagttgtgc tctttcgaga 420 tctacgaagt tccctgggag gacagaatgt ccctggtgaa ttccaggtgt caagaagcct 480 aggggtctgt gccaggccag tcacaccgac caccacccac tcccacccac tgtagtgctc 540 ccacccctgg actggtggcc cccaccctgc gggaggcctc cccatgtgcc tgtgccaa £ a 600 gacagacaga gaaggctgca ggagtccttt gttgctcagc agggcgctct gccctccctc 660 cttccttctt gcttctaata gacctggtac atggtacaca cacccccacc tcctgcaatt 720 aaacagtagc atcgcc 736 < 210 > 73 95238.doc - 19 - 1327645 <211> 2820 <212> DNA <213>Human<400> 73 ggcgggttcg cgccccgaag gctgagagct ggcgctgctc gtgccctgtg tgccagacgg 60 cggagctccg c £ gccggacc ccgcggcccc gctttgctgc cgact £ gagt ttgggggaag 120 aaactctcct gcgccccaga agatttcttc ctcggcgaag ggacagcgaa agatgagggt 180 ggcaggaaga gaaggcgctt tctgtctgcc ggggtcgcag cgcgagaggg cagtgccatg 240 ttcctctcca tcctagtggc gctgtgcctg tggctgcacc tggcgctggg cgtgcgcggc 300 gcgccctgcg aggcggtgcg catccctatg tgccggcaca tgccctggaa catcacgcgg 360 atgcccaacc acctgcacca cagcacgcag gagaacgcca tcctggccat cgagcagtac 420 gaggagctgg tggacgtgaa ctgcagcgcc gtgctgcgct tcttcttctg tgccatgtac 480 gcgcccattt gcaccctgga gttcctgcac gaccctatca agccgtgcaa gtcggtgtgc 540 caacgcgcgc gcgacgactg cgagcccctc atgaagatgt acaaccacag ctggcccgaa 600 agcctggcct gcgacgagct gcctgtctat gaccgtggcg tgtgcatttc gcctgaagcc 660 atcgtcacgg acctcccgga ggatgttaag tggatagaca tcacaccaga catgatggta 720 caggaaaggc ctcttgatgt tgactgtaaa cgcctaagcc ccgatcggtg caagtgtaaa 780 aaggtgaagc caactttggc aacgtatctc agcaaaaact acagctatgt tattcatgcc 840 aaaataaaag Ctgtgcagag gagtggctgc aatgaggtca caacggt ggt ggatgtaaaa 900 gagatcttca agtcctcatc acccatccct cgaactcaag tcccgctcat tacaaattct 960 tcttgccagt gtccacacat cctgccccat caagatgttc tcatcatgtg ttacgagtgg 1020 cgttcaagga tgatgcttct tgaaaattgc ttagttgaaa aatggagaga tcagcttagt 1080 aaaagatcca tacagtggga agagaggctg caggaacagc ggagaacagt tcaggacaag 1140 aagaaaacag ccgggcgcac cagtcgtagt aatcccccca aaccaaaggg aaagcctcct 1200 gctcccaaac cagccagtcc caagaagaac attaaaacta ggagtgccca gaagagaaca 1260 aacccgaaaa gagtgtgagc taactagttt ccaaagcgga gacttccgac Ttccttacag 1320 gatgaggctg ggcattgcct gggacagcct atgtaaggcc atgtgcccct tgccctaaca 1380 actcactgca gtgctcttca tagacacatc itgcagcatt tttcttaagg ctatgcttca 1440 gtttttcttt gtaagccatc acaagccata gtggtaggtt tgccctttgg tacagaaggt 1500 -20·

95238.doc 1327645 gagttaaagc tggtggaaaa ggcttattgc attgcattca gagtaacctg tgtgcatact 1560 ctagaagagt agggaaaata atgcttgtta caattcgacc taatatgtgc attgtaaaat 1620 aaatgccata tttcaaacaa aacacgtaat ttttttacag tatgttttat taccttttga 1680 tatctgttgt tgcaatgtta gtgatgtttt aaaatgtgat gaaaatataa tgtttttaag 1740 aaggaacagt agtggaatga atgttaaaag atctttatgt gtttatggtc tgcagaagga 1800 tttttgtgat gaaaggggat tttttgaaaa attagagaag tagcatatgg aaaattataa 1860 tgtgtttttt taccaatgac ttx: agtttct gtttttagct agaaacttaa aaacaaaaat 1920 aataataaag aaaaataaat aaaaaggaga ggcagacaat gtctggattc ctgttttttg 1980 gttacctgat ttccatgatc atgatgcttc ttgtcaacac cctcttaagc agcaccagaa 2040 acagtgagtt tgtctgtacc attaggagtt aggtactaat tagttggcta atgctcaagt 2100 attttatacc cacaagagag gtatgtcact catcttactt cccaggacat ccaccctgag 2160 aataatttga caagcttaaa aatggccttc atgtgagtgc caaattttgt ttttcttcat 2220 ttaaatattt tctttgccta aatacatgtg agaggagtta aatataaatg tacagagagg 2280 aaagttgagt tccacctctg aaatgagaat Tacttgacag ttgggatact tta atcagaa 2340 aaaaagaact tatttgcagc attttatcaa caaatttcat aattgtggac aattggaggc 2400 atttatttta aaaaacaatt ttattggcct tttgctaaca cagtaagcat gtattttata 2460 aggcattcaa taaatgcaca acgcccaaag gaaataaaat cctatctaat cctactctcc 2520 actacacaga ggtaatcact attagtattt tggcatatta ttctccaggt gtttgcttat 2580 gcacttataa aatgatttga acaaataaaa ctaggaacct gtatacatgt gtttcataac 2640 ctgcctcctt tgcttggccc tttattgaga taagttttcc tgtcaagaaa gcagaaacca 2700 tctcatttct aacagctgtg ttatattcca tagtatgcat tactcaacaa actgttgtgc 2760 tattggatac ttaggtggtt tcttcactga caatactgaa taaacatctc accggaattc 2820 <210> 74 <211> 2480 <212> DNA <213>human<400> 74 agtactaaca tggactaatc tgtgggagca gtttattcca gtatcaccca gggtgcagcc 60 -21 -

95238.doc 1327645 acaccaggac tgtgttgaag ggtgtttttt ttcttttaaa tgtaatacct cctcatcttt 120 tcttcttaca cagtgtctga gaacatttac attatagata agtagtacat ggtggataac 180 ttctactttt aggaggacta ctctcttctg acagtcctag actggtcttc tacactaaga 240 caccatgaag gagtatgtgc tcctattatt cctggctttg tgctctgcca aacccttctt 300 tagcccttca cacatcgcac tgaagaatat gatgctgaag gatatggaag acacagatga 360 tgatgatgat gatgatgatg atgatgatga tgatgatgag gacaactctc tttttccaac 420 aagagagcca agaagccatt tttttccatt tgatctgttt ccaatgtgtc catttggatg 480 tcagtgctat tcacgagttg tacattgctc agatttaggt ttgacctcag tcccaaccaa 540 cattccattt gatactcgaa tgcttgatct tcaaaacaat aaaattaagg aaatcaaaga 600 aaatgatttt aaaggactca cttcacttta tggtctgatc ctgaacaaca acaagctaac 660 gaagattcac ccaaaagcct ttctaaccac aaagaagttg cgaaggctgt atctgtccca 720 caatcaacta agtgaaatac cacttaatct tcccaaatca ttagcagaac tcagaattca 780 tgaaaataaa gttaagaaaa tacaaaagga cacattcaaa ggaatgaatg ctttacacgt 840 tttggaaatg agtgcaaacc ctcttgataa taatgggata gagccagggg Catttgaagg 900 gg tgacggtg ttccatatca gaattgcaga agcaaaactg acctcagttc ctaaaggctt 960 accaccaact ttattggagc ttcacttaga ttataataaa atttcaacag tggaacttga 1020 ggattttaaa cgatacaaag aactacaaag gctgggccta ggaaacaaca aaatcacaga 1080 tatcgaaaat gggagtcttg ctaacatacc acgtgtgaga gaaatacatt tggaaaacaa 1140 taaactaaaa aaaatccctt caggattacc agagttgaaa tacctccaga taatcttcct 1200 tcattctaat tcaattgcaa gagtgggagt aaatgacttc tgtccaacag tgccaaagat 1260 gaagaaatct ttatacagtg caataagttt attcaacaac ccggtgaaat actgggaaat 1320 gcaacctgca acatttcgtt gtgttttgag cagaatgagt gttcagcttg ggaactttgg 1380 aatgtaataa ttagtaattg gtaatgtcca tttaatataa gattcaaaaa tccctacatt 1440 tggaatactt gaactctatt aataatggta gtattatata tacaagcaaa tatctattct 1500 caagtggtaa gtccactgac ttattttatg acaagaaatt tcaacggaat tttgccaaac 1560 tattgataca taagggttga gagaaacaag catctattgc agtttctttt tgcgtacaaa 1620 tgatcttaca taaatctcat gcttgaccat tcctttcttc ataacaaaaa agtaagatat 1680 -22- 95238.doc 1327645 tcggtattta acactttgtt Atcaagcata ttttaaaaag aactgtactg ta aatggaat 1740 gcttgactta gcaaaatttg tgctctttca tttgctgtta gaaaaacaga attaacaaag 1800 acagtaatgt gaagagtgca ttacactatt ctlattcttt agtaacttgg gtagtactgt 1860 aatattttta atcatcttaa agtatgattt gatataatct tattgaaatt accttatcat 1920 gtcttagagc ccgtctttat gtttaaaact aatttcttaa aataaagcct tcagtaaatg 1980 ttcattacca acttgataaa tgctactcat aagagctggt ttggggctat agcatatgct 2040 tttttttttt taattattac ctgatttaaa aatctctgta aaaacgtgta gtgtttcata 2100 aaatctgtaa ctcgcatttt aatgatccgc tattataagc ttttaatagc atgaaaattg 2160 t Post gc coffee taacattgcc acttcaactc taaggaatat ttttgagata tccctttgga 2220 agaccttgct tggaagagcc tggacactaa caattctaca ccaaattgtc tcttcaaata 2280 cgtatggact ggataactct gagaaacaca tctagtataa ctgaataagc agagcatcaa 2340 attaaacaga cagaaaccga aagctctata taaatgctca gagttcttta tgtatttctt 2400 attggcattc aacatatgta aaatcagaaa acagggaaat tttcattaaa aatattggtt 2460 tgaaataaaa aaaaaaaaaa 2480 < 210 > 75 < 211 > 1887 <212> DNA <213> Human <400> 75 cgcgcagccc ctccggccgc gggcg cagcg ggggcgctgg tggagctgcg aagggccagg 60 tccggcgggc ggggcggcgg ctggcactgg ctccggactc tgcccggcca gggcggcggc 120 tccagccggg agggcgacgt ggagcggcca cgtggagcgg cccgggggag gctggcggcg 180 ggaggcgagg cgcgggcggc gcagcagcca ggagcgccca cggagctgga cccccagagc 240 cgcgcggcgc cgcagcagtt ccaggaagga tgttaccttt gacgatgaca gtgttaatcc 300 tgctgctgct ccccacgggt caggctgccc caaaggatgg agtcacaagg ccagactctg 360 aagtgcagca tcagctcctg cccaacccct tccagccagg ccaggagcag ctcggacttc 420 tgcagagcta cctaaaggga ctaggaagga cagaagtgca actggagcat ctgagccggg 480 agcaggttct cctctacctc tttgccctcc atgactatga ccagagtgga cagctggatg 540 gcctggagct gctgtccatg ttgacagctg ctctggcccc tggagctgcc aactctccta 600 • 23 · 95238.doc 1327645 ccaccaaccc ggtgatattg atagtggaca aagtgctcga gacgcaggac ctgaatgggg 660 atgggctcat gacccctgct gagctcatca acttcccggg agtagccctc aggcacgtgg 720 agcccggaga gccccttgct ccatctcctc aggagccaca agctgttgga aggcagtccc 780 tattagctaa aagcccatta agacaagaaa cacaggaagc Ccctggtccc agagaagaag 840 caaagggcca ggtagaggcc agaag ££ agt cttt £ gatcc tgtccaggag cctggg £ gcc 900 aggcagaggc tgatggagat gttccagggc ccagagggga agctgagggc caggcagagg 960 ctaaaggaga tgcccctggg cccagagggg aagctggggg ccaggcagag gctgaaggag 1020 atgcccccgg gcccagaggg gaagctgggg gccaggcaga ggccagggag aatggagagg 1080 aggccaagga acttccaggg gaaacactgg agtctaagaa cacccaaaat gactttgagg 1140 tgcacattgt tcaagtggag aatgatgaga tctagatctt gaagatacag gtaccccacg 1200 aagtctcagt gccagaacat aagccctgaa gtgggcaggg gaaatgtacg ctgggacaag 1260 gaccatctct gtgccccctg tctggtccca gtaggtatca ggtctttctg tgcagctcag 1320 ggagacccta agttaagggg cagattacca ataaagaact gaatgaattc atccccccgg 1380 gccacctctc tacccgtcca gcctgcccag accctctcag aggaacgggg ttggggaccg 1440 aaaggacagg gatgccgcct gcccagtgtt tctgggcctc acggtgctcc ggcagcagag 1500 cgcatggtgc tagccatggc cggctgcaga ggacccagtg aggaaagctc agtctatccc 1560 tgggccccaa accctcaccg gttccccctc acctggtgtt cagacacccc atgctctcct 1620 gcagctcagg gcaggtgacc ccatccccag taatattaat Catcactaga actttttgag 1680 agccttgtac acatcaggca tcat gctggg cattttatat atgattttat cctcacaata 1740 attctgtagc caagcagaat tggttccatt tgacagatga agaaattgag gcagattgcg 1800 ttaagtgctg taccctaagg tgatatgcag ctaattaaat ggcagatttg aaaaaaaaaa 1860 aaaaaaaaaa aaaaaaaaaa aaaaaaa 1887 < 210 > 76 < 211 > 1580 < 212 > DNA < 213 > human < 400 > 76 catcctgcca cccctagcct Tgctggggac gtgaaccctc tccccgcgcc tgggaagcct 60 -24-

95238.doc 1327645

tcttggcacc gggacccgga gaatccccac ggaagccagt tccaaaaggg atgaaaaggg 120 ggcgtttcgg gcactgggag aagcctgtat tccagggccc ctcccagagc aggaatctgg 180 gacccaggag tgccagcctc acccacgcag atcctggcca tgagagctcc gcacctccac 240 ctctccgccg cctctggcgc ccgggctctg gcgaagctgc tgccgctgct gatggcgcaa 300 ctctgggccg cagaggcggc gctgctcccc caaaacgaca cgcgcttgga ccccgaagcc 360 tatggctccc cgtgcgcgcg cggctcgcag ccctggcagg tctcgctctt caacggcctc 420 tcgttccact gcgcgggtgt cctggtggac cagagttggg tgctgacggc cgcgcactgc 480 ggaaacaagc cactgtgggc tcgagtaggg gatgaccacc tgctgcttct 540 cagctccgcc ggaccactcg ctctgttgtc catcccaagt accaccaggg ctcaggcccc 600 atcctgccaa ggcgaacgga tgagcacgat ctcatgttgc tgaagctggc caggcccgta 660 gtgctggggc cccgcgtccg ggccctgcag cttccctacc gctgtgctca gcccggagac 720 cagtgccagg ttgctggctg gggcaccacg gccgcccgga gagtgaagta caacaagggc 780 ctgacctgct ccagcatcac tatcctgagc cctaaagagt gtgaggtctt ctaccctggc 840 gtggtcacca acaacatgat atgtgctgga ctggaccggg gccaggaccc ttgccagagt 900 gactctggag gccccctgg tcagggagag t ctgtgacgag accctcxaag gcatcctctc gtggggtgtt 960 tacccctgtg gctctgccca gcatccagct gtctacaccc agatctgcaa atacatgtcc 1020 tggatcaata aagtcatacg ctccaactga tccagatgct acgctccagc tgatccagat 1080 gttatgctcc tgctgatcca gatgcccaga ggctccatcg tccatcctct tcctccccag 1140 tcggctgaac tctccccttg tctgcactgt tcaaacctct gccgccctcc acacctctaa 1200 acatctcccc tctcacctca ttcccccacc tatccccatt ctctgcctgt actgaagctg 1260 aaatgcagga agtggtggca aaggtttatt ccagagaagc caggaagccg gtcatcaccc 1320 agcctctgag agcagttact ggggtcaccc aacctgactt cctctgccac tccctgctgt 1380 gtgactttgg gcaagccaag tgccctctct gaacctcagt ttcctcatct gcaaaatggg 1440 aacaatgacg tgcctacctc ttagacatgt tgtgaggaga ctatgatata acatgtgtat 1500 gtaaatcttc atggtgattg tcatgtaagg cttaacacag tgggtggtga gttctgacta 1560 aaggttacct gttgtcgtga 1580 < 210 > 77 95238.doc - 25 - 1327645 < 211 > 1443 < 212> DNA <213>Human<400> 77 accagcggca gaccacaggc agggcagagg cacgtctggg tcccctccct ccttcctatc 60 ggcgactccc aggatcctgg ccatgagagc tccgcacc tc cacctctccg ccgcctctgg 120 cgcccgggct ctggcgaagc tgctgccgct gctgatggcg caactctggg ccgcagaggc 180 ggcgctgctc ccccaaaacg acacgcgctt ggaccccgaa gcctatggct ccccgtgcgc 240 gcgcggctcg cagccctggc aggtctcgct cttcaacggc ctctcgttcc actgcgcggg 300 tgtcctggtg gaccagagtt gggtgctgac ggccgcgcac tgcggaaaca agccactgtg 360 ggctcgagta ggggatgacc acctgctgct tcttcaggga gagcagctcc gccggaccac 420 tcgctctgtt gtccatccca agtaccacca gggctcaggc cccatcctgc caaggcgaac 480 ggatgagcac gatctcatgt tgctgaagct ggccaggccc gtagtgctgg ggccccgcgt 540 ccgggccctg cagcttccct accgctgtgc tcagcccgga gaccagtgcc aggttgctgg 600 ctggggcacc acggccgccc ggagagtgaa gtacaacaag ggcctgacct gctccagcat 660 cactatcctg agccctaaag agtgtgaggt cttctaccct ggcgtggtca ccaacaacat 720 gatatgtgct ggactggacc ggggccagga cccttgccag agtgactctg gaggccccct 780 ggtctgtgac gagaccctcc aaggcatcct ctcgtggggt gtttacccct gtggctctgc 840 ccagcatcca gctgtctaca cccagatctg caaatacatg tcctggatca ataaagtcat 900 acgctccaac tgatccagat gctacgctcc agctgatcca gatgttatgc Tcctgc tgat 960 ccagatgccc agaggctcca tcgtccatcc tcttcctccc cagtcggctg aactctcccc 1020 ttgtctgcac tgttcaaacc tctgccgccc tccacacctc taaacatctc ccctctcacc 1080 tcattccccc acctatcccc attctctgcc tgtactgaag ctgaaatgca ggaagtggtg 1140 gcaaaggttt attccagaga agccaggaag ccggtcatca cccagcctct gagagcagtt 1200 actggggtca cccaacctga cttcctctgc cactccctgc tgtgtgactt tgggcaagcc 1260 aagtgccctc tctgaacctc agtttcctca tctgcaaaat gggaacaatg acgtgcctac 1320 ctcttagaca tgttgtgagg agactatgat ataacatgtg tatgtaaatc ttcatggtga 1380 ttgtcatgta aggcttaaca cagtgggtgg tgagttctga ctaaaggtta cctgttgtcg 1440 tga 1443 -26·

95238.doc 1327645 < 210 > 78 < 211 > 782 < 212 > DNA < 213 > human < 400 > 78 aggggcctta gcgtgccgca tcgccgagat ccagcgccca gagagacacc agagaaccca 60 ccatggcccc ctttgagccc ctggcttctg gcatcctgtt gttgctgtgg ctgatagccc 120 ccagcagggc ctgcacctgt gtcccacccc acccacagac ggccttctgc aattccgacc 180 tcgtcatcag ggccaagttc gtggggacac cagaagtcaa ccagaccacc ttataccagc 240 gttatgagat caagatgacc aagatgtata aagggttcca agccttaggg gatgccgctg 300 acatccggtt cgtctacacc cccgccatgg agagtgtctg cggatacttc cacaggtccc 360 acaaccgcag cgaggagttt ctcattgctg gaaaactgca ggatggactc ttgcacatca 420 ctaectgcag tttcgtggct ccctggaaca gcctgagctt agctcagcgc cggggcttca 480 ccaagaccta cactgttggc tgtgaggaat gcacagtgtt tccctgttta tccatcccct 540 gcaaactgca gagtggcact cattgcttgt ggacggacca gctcctccaa ggctctgaaa 600 agggcttcca Gtcccgtcac cttgcctgcc tgcctcggga gccagggctg tgcacctggc 660 agtccctgcg gtcccagata gcctgaatcc tgcccggagt ggaactgaag cctgcacagt 720 gtccaccctg ttcccactcc catctttctt ccggacaatg aaataaagag ttaccacc ca 780 gc 782 < 210> 79 < 211 > 3178 < 212 > DNA < 213 > human < 400 > 79 gttgcctgtc tctaaacccc tccacattcc cgcggtcctt cagactgccc ggagagcgcg 60 ctctgcctgc cgcctgcctg cctgccactg agggttccca gcaccatgag ggcctggatc 120 ttctttctcc tttgcctggc cgggagggcc ttggcagccc ctcagcaaga agccctgcct 180 Gatgagacag aggtggtgga agaaactgtg gcagaggtga ctgaggtatc tgtgggagct 240 aatcctgtcc aggtggaagt aggagaattt gatgatggtg cagaggaaac cgaagaggag 300 gtggtggcgg aaaatccctg ccagaaccac cactgcaaac acggcaaggt gtgcgagctg 360 •27·

95238.doc 1327645 gatgagaaca acacccccat gtgcgtgtgc caggacccca ccagctgccc agcccccatt 420 ggcgagtttg agaaggtgtg cagcaatgac aacaagacct tcgactcttc ctgccacttc 480 tttgccacaa agtgcaccct ggagggcacc aagaagggcc acaagctcca cctggactac 540 atcgggcctt gcaaatacat ccccccttgc ctggactctg agctgaccga attccccctg 600 cgcatgcggg actggctcaa gaacgtcctg gtcaccctgt atgagaggga tgaggacaac 660 aaccttctga ctgagaagca gaagctgcgg gtgaagaaga iccatgagaa tgagaagcgc 720 ctggaggcag gagaccaccc cgtggagctg ctggcccggg acttcgagaa gaactataac 780 atgtacatct tccctgtaca ctggcagttc ggccagctgg accagcaccc cattgacggg 840 tacctctccc acaccgagct ggctccactg cgtgctcccc tcatccccat ggagcattgc 900 accacccgct ttttcgagac ctgtgacctg gacaatgaca agtacatcgc cctggatgag 960 tgggccggct gcttcggcat caagcagaag gatatcgaca aggatcttgt gatctaaatc 1020 cactccttcc acagtaccgg attctctctt taaccctccc cttcgtgttt cccccaatgt 1080 ttaaaatgtt tggatggttt gttgttctgc ctggagacaa ggtgctaaca tagatttaag 1140 tgaatacatt aacggtgcta aaaatgaaaa ttctaaccca agacatgaca Ttcttagctg 120 0 taacttaact attaaggcct tttccacacg cattaatagt cccatttttc tcttgccatt 1260 tgtagctttg cccattgtct tattggcaca tgggtggaca cggatctgct gggctctgcc 1320 ttaaacacac attgcagctt caacttttct ctttagtgtt ctgtttgaaa ctaatactta 1380 ccgagtcaga ctttgtgttc atttcatttc agggtcttgg ctgcctgtgg gcttccccag 1440 gtggcctgga ggtgggcaaa gggaagtaac agacacacga tgttgtcaag gatggttttg 1500 ggactagagg ctcagtggtg four agagatcc ctgcagaacc caccaaccag aacgtggttt 1560 gcctgaggct gtaactgaga gaaagattct ggggctgtgt tatgaaaata tagacattct 1620 cacataagcc cagttcatca ccatttcctc ctttaccttt cagtgcagtt tcttttcaca 1680 ttaggctgtt ggttcaaact tttgggagca cggactgtca gttctctggg aagtggtcag 1740 cgcatcctgc agggcttctc ctcctctgtc ttttggagaa ccagggctct tctcaggggc 1800 tctagggact gccaggctgt ttcagccagg aaggccaaaa tcaagagtga gatgtagaaa 1860 gttgtaaaat agaaaaagtg gagttggtga atcggttgtt ctttcctcac atttggatga 1920 ttgtcataag gtttttagca tgttcctcct tttcttcacc ctcccctttt ttcttctatt 1980 28 ·

95238.doc 1327645 aatcaagaga aacttcaaag ttaatgggat ggtcggatct cacaggctga gaactcgttc 2040 acctccaagc atttcatgaa aaagctgctt cttattaatc atacaaactc tcaccatgat 2100 gtgaagagtt tcacaaatcc ttcaaaataa aaagtaatga cttagaaact gccttcctgg 2160 gtgatttgca tgtgtcttag tcttagtcac cttattatcc tgacacaaaa acacatgagc 2220 atacatgtct acacatgact acacaaatgc aaacctttgc aaacacatta tgcttttgca 2280 cacacacacc tgtacacaca caccggcatg tttatacaca gggagtgtat ggttcctgta 2340 agcactaagt tagctgttu; catttaatga cctgtggttt aacccttttg atcactacca 2400 ccattatcag caccagactg agcagctata tccttttatt aatcatggtc attcattcat 2460 tcattcattc acaaaatatt tatgatgtat ttactctgca ccaggtccca tgccaagcac 2520 tggggacaca gttatggcaa agtagacaaa gcatttgttc atttggagct tagagtccag 2580 gaggaataca ttagataatg acacaatcaa atataaattg caagatgtca caggtgtgat 2640 gaagggagag taggagagac catgagtatg tgtaacagga ggacacagca ttattctagt 2700 gctgtactgt tccgtac £ gc agccactacc cacatgtaac tttttaagat ttaaatttaa 2760 attagttaac attcaaaacg Cagctcccca atcacactag caacatttca a gtgcttgag 2820 agccatgcat gattagtggt taccctattg aataggtcag aagtagaatc ttttcatcat 2880 cacagaaagt tctattggac agtgctcttc tagatcatca taagactaca gagcactttt 2940 caaagctcat gcatgttcat catgttagtg tcgtattttg agctggggtt ttgagactcc 3000 ccttagagat agagaaacag acccaagaaa tgtgctcaat tgcaatgggc cacataccta 3060 gatctccaga tgtcatttcc cctctcttat tttaagttat gttaagatta ctaaaacaat 3120 aaaagctcct aaaaaatcaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa 3178 < 210 > 80 < 211 > 2691 < 212 > DNA < 213 > human < 400 > 80 gcttgcccgt cggtcgctag ctcgctcggt gcgcgtcgtc ccgctccatg gcgctcttcg 60 tgcggctgct ggctctcgcc ctggctctgg ccctgggccc cgccgcgacc ctggcgggtc 120 ccgccaagtc gccctaccag ctggtgctgc agcacagcag gctccggggc cgccagcacg 180 -29- 95238.doc 1327645 gccccaacgt gtgtgctgtg cagaaggtta Ttggcactaa taggaagtac ttcaccaact 240 gcaagcagtg gtaccaaagg aaaatctgtg gcaaatcaac agtcatcagc tacgagtgct 300 gtcctggata tgaaaaggtc cctggggaga agggctgtcc agcagcccta ccactctcaa 360 acctttacga gaccctggga gtcgttggat ccacc accac tcagctgtac acggaccgca 420 cggagaagct gaggcctgag atggaggggc ccggcagctt caccatcttc gcccctagca 480 acgaggcctg ggcctccttg ccagctgaag tgctggactc cctggtcagc aatgtcaaca 540 ttgagctgct caatgccctc cgctaccata tggtgggcag gcgagtcctg actgatgagc 600 tgaaacacgg catgaccctc acctctatgt accagaattc caacatccag atccaccact 660 atcctaatgg gattgtaact gtgaactgtg cccggctcct gaaagccgac caccatgcaa 720 ccaacggggt ggtgcacctc atcgataagg tcatctccac catcaccaac aacatccagc 780 agatcattga gatcgaggac acctttgaga cccttcgggc tgctgtggct gcatcagggc 840 tcaacacgat gcttgaaggt aacggccagt acacgctttt ggccccgacc aatgaggcct 900 tcgagaagat ccctagtgag actttgaacc gtatcctggg cgacccagaa gccctgagag 960 acctgctgaa caaccacatc ttgaagtcag ctatgtgtgc tgaagccatc gttgcggggc 1020 tgtctgtaga gaccctggag ggcacgacac tggaggtggg ctgcagcggg gacatgctca 1080 ctatcaacgg gaaggcgatc atctccaata aagacatcct agccaccaac ggggtgatcc 1140 actacattga tgagctactc atcccagact cagccaagac actatttgaa ttggctgcag 1200 agtctgatgt gtccacagcc attgaccttt tcagacaagc cggcctcggc aatcatctct 1260 ctggaagtga gcggttgacc ctcctggctc ccctgaattc tgtattcaaa gatggaaccc 1320 ctccaattga tgcccataca aggaatttgc ttcggaacca cataattaaa gaccagctgg 1380 cctctaagta tctgtaccat ggacagaccc tggaaactct gggcggcaaa aaactgagag 1440 tttttgttta tcgtaatagc ctctgcattg agaacagctg catcgcggcc cacgacaaga 1500 gggggaggta cgggaccctg ttcacgatgg accgggtgct gaccccccca atggggactg 1560 tcatggatgt cctgaaggga gacaatcgct ttagcatgct ggtagctgcc atccagtctg 1620 ca £ gact £ ac ggagaccctc Aaccgggaag gagtctacac agtctttgct cccacaaatg 1680 aagccttccg agccctgcca ccaagagaac ggagcagact cttgggagat gccaaggaac 1740 ttgccaacat cctgaaatac cacattggtg atgaaatcct ggttagcgga ggcatcgggg 1800 •30·

95238.doc 1327645 ccctggtgcg gctaaagtct ctccaaggtg acaagctgga agtcagcttg aaaaacaatg 1860 tggtgagtgt caacaaggag cctgttgccg agcctgacat catggccaca aatggcgtgg 1920 tccatgtcat caccaatgtt ctgcagcctc cagccaacag acctcaggaa agaggggatg 1980 aacttgcaga ctctgcgctt gagatcttca aacaagcatc agcgttttcc agggcttccc 2040 agaggtctgt gcgactagcc cctgtctatc aaaagttatt agagaggatg aagcattagc 2100 ttgaagcact acaggaggaa tgcaccacgg cagctctccg ccaatttctc tcagatttcc 2160 acagagactg tttgaatgtt ttcaaaacca agtatcacac tttaatgtac atgggccgca 2220 ccataatgag atgtgagcct tgtgcatgtg ggggaggagg gagagagatg tactttttaa 2280 atcatgttcc ccctaaacat ggctgttaac ccactgcatg cagaaacttg gatgtcactg 2340 cctgacattc acttccagag aggacctatc ccaaatgtgg aattgactgc ctatgccaag 2400 tccctggaaa agtttttgta aggagcttca gtattgtggg gctcataaaa catgaatcaa gcaatccagc 2460 ctcatgggaa gtcctggcac aggcttttat ggggccctgt ccaggtagaa aagcccttgc acagctggag aaatggcatc 2520 attataagct atgagttgaa atgttctgtc aaatgtgtct cacatctaca cgtggcttgg 2580 aagaaatggt atgtagagct Taga tttccc 2640 tattgtgaca gagccatggt gtgtttgtaa taataaaacc aaagaaacat a 2691 < 210 > 81 < 211 > 1757 < 212 > DNA < 213 > human < 400> 81 caagcttggc acgagggcag gcattgcccg agccagccga gccgccagag ccgcgggccg 60 cgcgggtgtc gcgggcccaa ccccaggatg ctcccctgcg cctcctgcct acccgggtct 120 ctactgctct gggcgctgct actgttgctc ttgggatcag cttctcctca ggattctgaa 180 gagcccgaca gctacacgga atgcacagat ggctatgagt gggacccaga cagccagcac 240 tgccgggatg tcaacgagtg tctgaccatc cctgaggcct gcaaggggga aatgaagtgc 300 atcaaccact acgggggcta cttgtgcctg ccccgctccg ctgccgtcat caacgaccta 360 cacggcgagg gacccccgcc accagtgcct cccgctcaac accccaaccc ctgcccacca 420 ggctatgagc ccgacgatca ggacagctgt gtggatgtgg acgagtgtgc ccaggccctg 480 cacgactgtc gccccagcca ggactgccat aacttgcctg gctcctatca gtgcacctgc 540 -31 ·

95238.doc 1327645 cctgatggtt accgcaagat cgggcccgag tgtgtggaca tagacgagtg ccgctaccgc 600 tactgccagc accgctgcgt gaacctgcct ggctccttcc gctgccagtg cgagccgggc 660 ttccagctgg ggcctaacaa ccgctcctgt gttgatgtga acgagtgtga catgggggcc 720 ccatgcgagc agcgctgctt caactcctat gggaccttcc tgtgtcgctg ccaccagggc 780 tatgagctgc atcgggatgg cttctcctgc agtgatattg atgagtgtag ctactccagc 840 tacctctgtc agtaccgctg cgtcaacgag ccaggccgtt tctcctgcca ctgcccacag 900 ggttaccagc tgctggccac acgcctctgc caagacattg atgagtgtga gtctggtgcg 960 caccagtgct ccgaggccca aacctgtgtc aacttccatg ggggctaccg ctgcgtggac 1020 accaaccgct gcgtggagcc ctacatccag gtctctgaga accgctgtct ctgcccggcc 1080 tccaaccctc tatgtcgaga gcagccttca tccattgtgc accgctacat gaccatcacc 1140 tcggagcgga gagtacccgc tgacgtgttc cagatccagg cgacctccgt ctaccccggt 1200 gcctacaatg cctttcagat ccgtgctgga aactcgcagg gggactttta cattaggcaa 1260 atcaacaacg tcagcgccat gctggtcctc gcccggccgg tgacgggccc ccgggagtac 1320 gtgctggacc tggagatggt caccatgaat tccctcatga gctaccgggc Cagctctgta 1380 ctgaggctca ccgtctttgt aggggcctac accttctgag gagcaggagg gagccaccct 1440 ccctgcagct accctagctg aggagcctgt tgtgaggggc agaatgagaa aggcccaggg 1500 gcccccattg acaggagctg ggagctctgc accacgagct tcagtcaccc cgagaggaga 1560 ggaggtaacg aggagggcgg actccaggcc ccggcccaga gatttggact tggctggctt 1620 gcaggggtcc taagaaactc cactctggac agcgccagga ggccctgggt tccattccta 1680 actctgcctc aaactgtaca tttggataag ccctagtagt tccctgggcc tgtttttcta 1740 taaaacgagg caactgg 1757 < 210 > 82 <211> 1804 <212> DNA <213> human <400> 82 gtatcactca gaatctggca gccagttccg tcctgacaga gttcacagca tatattggtg 60 gattcttgtc catagtgcat ctgctttaag aattaacgaa agcagtgtca agacagtaag 120 -32-

95238.doc 1327645 gattcaaacc atttgccaaa aatgagtcta agtgcattta ctctcttcct ggcattgatt 180 ggtggtacca gtggccagta ctatgattat gattttcccc tatcaattta tgggcaatca 240 tcaccaaact gtgcaccaga atgtaactgc cctgaaagct acccaagtgc catgtactgt 300 gatgagctga aattgaaaag tgtaccaatg gtgcctcctg gaatcaagta tctttacctt 360 aggaataacc agattgacca tattgatgaa aaggcctttg agaatgtaac tgatctgcag 420 tggctcattc tagatcacaa ccttctagaa aactccaaga taaaagggag agttttctct 480 aaattgaaac aactgaagaa gctgcatata aaccacaaca acctgacaga gtctgtgggc 540 ccacttccca aatctctgga ggatctgcag cttactcata acaagatcac aaagctgggc 600 tcttttgaag gattggtaaa cctgaccttc atccatctcc agcacaatcg gctgaaagag 660 gatgctgttt cagctgcttt taaaggtctt aaatcactcg aataccttga cttgagcttc 720 aatcagatag ccagactgcc ttctggtctc cctgtctctc ttctaactct ctacttagac 780 aacaataaga tcagcaacat ccctgatgag tatttcaagc gttttaatgc attgcagtat 840 ctgcgtttat ctcacaacga actggctgat agtggaatac ctggaaattc tttcaatgtg 900 tcatccctgg ttgagctgga tctgtcctat aacaagctta aaaacatacc Aactgtcaat 960 ga aaaccttg aaaactatta cctggaggtc aatcaacttg agaagtttga cataaagagc 1020 ttctgcaaga tcctggggcc attatcctac tccaagatca agcatttgcg tttggatggc 1080 aatcgcatct cagaaaccag tcttccaccg gatatgtatg aatgtctacg tgttgctaac 1140 gaagtcactc ttaattaata tctgtatcct ggaacaatat tttatggtta tgtttttctg 1200 tgtgtcagtt ttcatagtat ccatatttta ttactgttta ttacttccat gaattttaaa 1260 atctgaggga aatgttttgt aaacatttat tttttttaaa gaaaagatga aaggcaggcc 1320 tatttcatca caagaacaca cacatataca cgaatagaca tcaaactcaa tgctttattt 1380 gtaaatttag tgttttttta tttctactgt caaatgatgt gcaaaacctt ttactggttg 1440 catggaaatc agccaagttt tataatcctt aaatcttaat gttcctcaaa gcttggatta 1500 aatacatatg gatgttactc tcttgcacca aattatcttg atacattcaa atttgtctgg 1560 ttaaaaaata ggtggtagat attgaggcca agaatattgc aaaatacatg aagcttcatg 1620 cacttaaaga agtattttta gaataagaat ttgcatactt acctagtgaa acttttctag 1680 aattattttt cactctaagt catgtatgtt tctctttgat tatttgcatg ttatgtttaa 1740 -33-

95238.doc 1327645 taagctacta gcaaaataaa acatagcaaa tgaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1800 aaaa 1804 < 210 > 83 < 211 > 3290 < 212 > DNA < 213 > human < 400 > 83 agcggggccg gaccgggcgg gcggagccgg gcccgcgggg ctgctgcggg gceatcessc 60 cgggccgctg ccgcgccatg gactcccgtg tccagcctga gttccagcct cactgagtgg 120 ccacccccaa agtgctgcca gccgaggaag cccccagcac tgaccatgtc tattatggac 180 cacagcccca ccacgggcgt ggtcacagtc atcgtcatcc tcattgccat cgcggccctg 240 ggggccttga tcctgggctg ctggtgctac ctgcggctgc agcgcatcag ccagtcagag 300 gacgaggaga gcatcgtggg ggatggggag accaaggaac ccttcctgct ggtgcagtat 360 tcggccaagg gaccgtgcgt ggagagaaag gccaagctga tgactcccaa cggcccggaa 420 gtccacggct gagccaggat gcaaggctcc tggtcctgtt tgcagccggc caagaggcgc 480 tgggaggggc aaaaccatac ggatgcgctg ctgtctgaga ggaagggctg acacttgctg 540 gcatggcctc tgcgggcttc gtcatcgcat gcactgatgc ccggggacct ggctgtcctg 600 ggcttcccct cggcctccag gtgaggctgc ccattgcagg cactgggcag gcctgacctt 660 gctggggctc atggccctgt agcgcttttg ttacttgaat gtctagc tga gcctgttttt 720 gatggagcta ctactgtaat gcgtgaacta acaaacctgt gaactgtaaa taggcccctg 780 gaagcacgtg cttaagccct tttgctgatt tttaaaaata tcatctagcg cacacgggac 840 tggtattctg gctgtactaa tgacaagctg agtcaagacc ctggagggtc ataggcttgt 900 aaaggcccac gccacactcg gcaggggtct ctcatgtgtg tccatctgcg tgtatgtcaa 960 ggaagtgaga tgccaatttg gggtcttgag gctgaccagt tggggtgctt gggtgatctc 1020 tgcttcatta gtcatgggtg gaagaaaaac cacacccccc gcacccctcc gttctttctg 1080 catagactca cttgttaaat agcagttctg ttgagagtgg agttactgca Gggaagctac 1140 cggacctgcc tgggagccag tgaagggcga gtcagggcac gcgtcctgga ggctgccagc 1200 gtccttgtag cagagcagtt tcttgccgct tgggtctica gcacgccaag ccccccacca 1260 accctccacc ccgagtgaag gcttcgctga aattgctttg gtcctcatag agcctgtggt 1320 -34·

95238.doc 1327645 ggctactttt ggtctgaaac ccacttggcc caggaaagag aaaaggttgt atgttttgtg 1380 ttggtgtttc ctattttct £ cactggaggg gaggggactg ttgaggttct gtcttttttc 1440 ticttttcct cttccctctt cacatcactt ggcttccttt cctctctgat gaccgtccgc 1500 ctatggggtt ctgacttcac tttcctcagc gggtctccag tcccctgacc cagctctaaa 1560 ggcacttagg acccagggaa catttctcac gtgcacattc ccctaagagc caccagactg 1620 cttcctgcca gcctgtgctt gcggcaggga gccggggcag ggcagaggtg aacttgaagt 1680 tcaggacttg actctcccac aggtggtgag ctggtggctc tctggtgagc tagtgtctcc 1740 acagcctgtc tccaaggcct cccctatgta catttcagtg agctcacttt gatttttaat 1800 cccaccacaa gcacatacta attttattta tgattcaaat gtgactcgtg cctgcccatc 1860 cctgtaatag atggaaggtc agccccggct taaccacaga gcactggccc ttcatggctg 1920 agctcagagc tctggcctcc tgctcagact aaaggcacct cctctggcct cacccaagcc 1980 tcttctaaaa accatgttga atgaatccac gttctggaac cccgaggcgg gagaagtagg 2040 gagctgttcg tttaagcagc atacacctaa attgggggtt taaacattaa gtaggagctt 2100 ggggtggaag agggacagcc ggctgggcca cctgagcaga Aggtggtaat gaa acacctc 2160 agctgggctc ttgggagacc ttaggaagca ggagaggcaa cacctctggc tactgatggt 2220 gtggcaagtt cagaagaggt ggtggtgggg taggcgtgat gtcagcagaa gccctgcagg 2280 ctgggtgggc aggacacgtg gtgggggcca ctgaaaccag gcctaggagg gagaacaagt 2340 tccaaaggtg ccgactggaa gaagggggta aaagtttgct ttggtgagtg agaaaaggct 2400 ggggcgtgtg atccatcccc tcacgtttca gaacttccag gctttctacc tcgactctca 2460 ccacagccag cacatacacc taggctgttt ttccttcctc cacacctgag ggacgcagca 2520 acagctagga tctgcatttt caggttccga gcctgacccc tggaactgac cagcgctcga 2580 ttgtcagcct tggcctgggg ttttgacctt gccagtgaag tttcggtttt gaagtgatta 2640 aatgtcactt cctcatcagt ttcacttctg gaggttttct tatcctactc cctggtgcca 2700 gggacgtacc tgggagtttg aatcaggccc atttgagcgt ggcagccgtg ttgggtgaag 2760 gtccggggct cggtgaggca ctgggggggt tttcgggagg aaaatgaaaa tgcttctaga 2820 atgagtgaac cacatcatag ctctcactgt tttttcaata gctacttttt ttagcagaca 2880 ccagagccac actcaaatgg ctaagtaggt tatgacctct ctggattatt tttgaatgcc 2940 -35-

95238.doc 1327645 caactgttgc attcaagttt tctgactaat aagaaattaa gcattcatcc ttcgtatcac 3000 tgcagaagca acagtggggg cacagggagg gaactcttga cactgagcca ctaaaatatg 3060 gactaatttt ttggacaaat cttcaaacgg actgtgctac tgtatttgtc tcaaagctac 3120 caagtttgtg caataagtgg aagggatgtc atccttcttc aataaatgct gaatgacatt 3180 caagctgatt ttctagacca ctgagaaaat ctttatttac aataaatttc aataaaattt 3240 gcataaatat attcccaaaa aaaaaaaaaa aaaaaaagaa aaaaaaaaaa 3290 < 210> 84 agttctgagg aaaagcagct 84 ctccctgtgt tggtggagga tgtctgcagc agcatttaaa ttctgggagg gcttggttgt 60 cagcagcagc aggaggaggc agagcacagc atcgtcggga ccagactcgt ctcaggccag 120 ttgcagcctt ctcagccaaa cgccgaccaa ggaaaactca ctaccatgag aattgcagtg 180 atttgctttt gcctcctagg catcacctgt gccataccag ttaaacaggc tgattctgga 240; < 211 > 1616 < 212 > DNA < 213 > human < 400 & gt Ttacaacaaa tacccagatg ctgtggccac atggctaaac 300 cctgacccat ctcagaagca gaatctccta gccccacaga cccttccaag taagtccaac 360 gaaagccatg accacatgga tgatatggat gatgaagatg atgatgacca tgtggacagc 42 0 caggactcca ttgactcgaa cgactctgat gatgtagatg acactgatga ttctcaccag 480 tctgatgagt ctcaccattc tgatgaatct gatgaactgg tcactgattt tcccacggac 540 ctgccagcaa ccgaagtttt cactccagtt gtccccacag tagacacata tgatggccga 600 ggtgatagtg tggtttatgg actgaggtca aaatctaaga agtttcgcag acctgacatc 660 cagtaccctg atgctacaga cgaggacatc acctcacaca tggaaagcga ggagttgaat 720 ggtgcataca aggccatccc cgttgcccag gacctgaacg cgccttctga ttgggacagc 780 cgtgggaagg acagttatga aacgagtcag ctggat £ acc agagtgctga Acaccacagc 840 cacaagcagt ccagattata taagcggaaa gccaatgatg agagcaatga gcattccgat 900 gtgattgata gtcaggaact ttccaaagtc agccgtgaat tccacagcca tgaatttcac 960 agccatgaag atatgctggt tgtagacccc aaaagtaagg aagaagataa acacctgaaa 1020 •36-

95238.doc 1327645 tttcgtattt ctcatgaatt agatagtgca tcttctgagg tcaattaaaa ggagaaaaaa 1080 tacaatttct cactttgcat ttagtcaaaa gaaaaaatgc tttatagcaa aatgaaagag 1140 aacatgaaat gcttctttct cagtttattg gttgaatgtg tatctatttg agtctggaaa 1200 taactaatgt gtttgataat tagtttagtt tgtggcttca tggaaactcc ctgtaaacta 1260 aaagcttcag ggttatgtct atgttcattc tatagaagaa atgcaaacta tcact £ tatt 1320 ttaatatttg ttattctctc atgaatagaa atttatgtag aagcaaacaa aatactttta 1380 cccacttaaa aagagaatat aacattttat gtcactataa tcttttgttt tttaagttag 1440 tgtatatttt gttgtgatta tctttttgtg gtgtgaataa atcttttatc ttgaatgtaa 1500 taagaatttg gtggtgtcaa ttgcttattt gttttcccac ggttgtccag caattaataa 1560 aacat of cct tttttactgc ct made aaaa made aaaaaaa of a aaaaaaaaaa aa sister aa 1616 < 210 > 85 < 211 > 11185 < 212 > DNA <213>Human<400> 85 gctgccccga gcctttctgg ggaagaactc caggcgtgcg gacgcaacag ccgagaacat 60 taggtgttgt ggacaggagc tgggaccaag atcttcggcc agccccgcat cctcccgcat 120 cttccagcac cgtcccgcac cctccgcatc cttccccggg ccacca cgct tcctatgtga 180 cccgcctggg caacgccgaa cccagtcgcg cagcgctgca gtgaattttc cccccaaact 240 gcaataagcc gccttccaag gccaagatgt tcataaatat aaagagcatc ttatggatgt 300 gttcaacctt aatagtaacc catgcgctac ataaagtcaa agtgggaaaa agcccaccgg 360 tgaggggctc cctctctgga aaagtcagcc taccttgtca tttttcaacg atgcctactt 420 tgccacccag ttacaacacc agtgaatttc tccgcatcaa atggtctaag attgaagtgg 480 acaaaaatgg aaaagatttg aaagagacta ctgtccttgt ggcccaaaat ggaaatatca 540 agattggtca ggactacaaa gggagagtgt ctgtgcccac acatcccgag gctgtgggcg 600 atgcctccct cactgtggtc aagctgctgg caagtgatgc gggtctttac cgctgtgacg 660 tcatgtacgg gattgaagac acacaagaca cggtgtcact gactgtggat ggggttgtgt 720 ttcactacag ggcggcaacc agcaggtaca cactgaattt tgaggctgct cagaaggctt 780 gtttggacgt tggggcagtc atagcaactc cagagcagct ctttgctgcc tatgaagatg 840 -37-

95238.doc 1327645 gatttgagca gtgtgacgca ggctggctgg ctgatcagac tgtcagatat cccatccggg 900 ctcccagagt aggctgttat ggagataaga tgggaaaggc aggagtcagg acttatggat 960 tccgttctcc ccaggaaact tacgatgtgt attgttatgt ggatcatctg gatggtgatg 1020 tgttccacct cactgtcccc agtaaattca ccttcgagga ggctgcaaaa gagtgtgaaa 1080 accaggatgc caggctggca acagtggggg aactccaggc ggcatggagg aacggctttg 1140 accagtgcga ttacgggtgg ctgtcggatg ccagcgtgcg ccaccctgtg actgtggcca 1200 g £ gcccagtg tggaggtggt ctacttgggg tgagaaccct gtatcgtttt gagaaccaga 1260 caggcttccc tccccctgat agcagatttg atgcctactg ctttaaacct aaagaggcta 1320 caaccatcga tttgagtatc ctcgcagaaa ctgcatcacc cagtttatcc aaagaaccac 1380 aaatggtttc tgatagaact acaccaatca tccctttagt tgatgaatta cctgtcattc 1440 caacagagtt ccctcccgtg ggaaatattg tcagttttga acagaaagcc acagtccaac 1500 ctcaggctat cacagatagt ttagccacca aattacccac acctactggc agtaccaaga 1560 agccctggga tatggatgac tactcacctt ctgcttcagg acctcttgga aagctagaca 1620 tatcagaaat taaggaagaa gtgctccaga Gtacaactgg cgtctctcat tatgc tacgg 1680 attcatggga tggtgtcgtg gaagataaac aaacacaaga atcggttaca cagattgaac 1740 aaatagaagt gggtcctttg gtaacatcta tggaaatctt aaagcacatt ccttccaagg 1800 aattccctgt aactgaaaca ccattggtaa ctgcaagaat gatcctggaa tccaaaactg 1860 aaaagaaaat ggtaagcact gtttctgaat tggtaaccac aggtcactat ggattcacct 1920 tgggagaaga ggatgatgaa gacagaacac ttacagttgg atctgatgag agcaccttga 1980 tctttgacca aattcctgaa gtcattacgg tgtcaaagac ttcagaagac accatccaca 2040 ctcatttaga agacttggag tcagtctcag catccacaac tgtttcccct ttaattatgc 2100 ctgataataa tggatcatcc atggatgact gggaagagag acaaactagt ggtaggataa 2160 cggaagagtt tcttggcaaa tatctgtcta ctacaccttt tccatcacag catcgtacag 2220 aaatagaatt gtttccttat tctggtgata aaatattagt agagggaatt tccacagtta 2280 tttatccttc tctacaaaca gaaatgacac atagaagaga aagaacagaa acactaatac 2340 cagagatgag aacagatact tatacagatg aaatacaaga agagatcact aaaagtccat 2400 ttatgggaaa aacagaagaa gaagtcttct ctgggatgaa actctctaca tctctctcag 2460 -38 ·

95238.doc 1327645 agccaattca tgttacagag tcttctgtgg aaatgaccaa gtcttttgat ttcccaacat 2520 tgataacaaa gttaagtgca gagccaacag aagtaagaga tatggaggaa gactttacag 2580 caactccagg tactacaaaa tatgatgaaa atattacaac agtgcttttg gcccatggta 2640 ctttaagtgt tgaagcagcc actgtatcaa aatggtcatg ggatgaagat aatacaacat 2700 ccaagccttt agagtctaca gaaccttcag cctcttcaaa attgccccct gccttactca 2760 caactgtggg gatgaatgga aaggataaag acatcccaag tttcactgaa gatggagcag 2820 atgaatttac tcttattcca gatagtactc aaaagcagtt agaggaggtt 2880 acatagcagc ccatggaaaa ttcacaatta gatttcagcc aactacatca actggtattg 2940 cagaaaagtc aactttgaga gattctacaa ctgaagaaaa agttccacct atcacaagca 3000 ctgaaggcca agtttatgca accatggaag gaagtgcttt gggtgaagta gaagatgtgg 3060 acctctctaa gccagtatct actgttcccc aatttgcaca cacttcagag gtggaaggat 3120 tagcatttgt tagttatagt agcacccaag agcctactac ttatgtagac tcttcccata 3180 ccattcctct ttctgtaatt cccaagacag actggggagt gttagtacct tctgttccat 3240 cagaagatga agttctaggt gaaccctctc aagacatact tgtcattgat actgatgaag Caga ctcgcc 3300 ttgaagcgac tatttctcca gaaactatga gaacaacaaa aatcacagag ggaacaactc 3360 aggaagaatt cccttggaaa gaacagactg cagagaaacc agttcctgct ctcagttcta 3420 cagcttggac tcccaaggag gcagtaacac cactggatga acaagagggc gatggatcag 3480 catatacagt ctctgaagat gaattgttga caggttctga gagggtccca gUttagaaa 3540 caactccagt tggaaaaatt gatcacagtg tgtcttatcc accaggtgct gtaactgagc 3600 acaaagtgaa aacagatgaa gtggtaacac taacaccacg cattgggcca aaagtatctt 3660 taagtccagg gcctgaacaa aaatatgaaa cagaaggtag tagtacaaca ggatttacat 3720 catctttgag tccttttagt acccacatta cccagcttat ggaagaaacc actactgaga 3780 aaacatccct agaggatatt gatttaggct caggattatt tgaaaagccc aaagccacag 3840 aactcataga attttcaaca atcaaagtca cagttccaag tgatattacc actgccttca 3900 gttcagtaga cagacttcac acaacttcag cattcaagcc atcttccgcg atcactaaga 3960 aaccacctct catcgacagg gaacctggtg aagaaacaac cagtgacatg gtaatcattg 4020 gagaatcaac atctcatgtt cctcccacta cccttgaaga tattgtagcc aaggaaacag 4080 -39-

95238.doc 1327645 aaaccgatat tgatagagag tatttcacga cttcaagtcc tcctgctaca cagccaacaa 4140 gaccacccac tgtggaagac aaagaggcct ttggacctca ggcgctttct 4200 ccccagcaag cacaaaattt caccctgaca ttaatgttta tattattgag £ tcagagaaa 4260 ataagacagg tcgaatgagt gatttgagtg taattggtca tccaatagat tcagaatcta 4320 aagaagatga accttgtagt gaagaaacag atccagtgca tgatctaatg gctgaaattt 4380 tacctgaatt ccctgacata attgaaatag acctatacca cagtgaagaa aatgaagaag 4440 aagaagaaga gtgtgcaaat gctactgatg acgccacagc tgacaaccac cccatctgtg cagtacataa 4500 atgggaagca tctcgttacc actgtgccca aggacccaga agctgcagaa gctaggcgtg 4560 gccagtttga aagtgttgca ccttctcaga atttctcgga cagctctgaa agtgatactc 4620 atccatttgt aatagccaaa acggaattgt ctactgctgt gcaacctaat gaatctacag 4680 aaacaactga gtctcttgaa gttacatgga agcctgagac ttaccctgaa acatcagaac 4740 atttttcagg tggtgagcct gatgttttcc ccacagtccc attccatgag gaatttgaaa 4800 gtggaacagc caaaaaaggg gcagaatcag tcacagagag agatactgaa gttggtcatc 4860 aggcacatga acatactgaa cctgtatctc tgtttcctga Agaggtctca gga gagattg 4920 ccattgacca agaatctcag aaaatagcct ttgcaagggc tacagaagta acatttggtg 4980 aagaggtaga aaaaagtact tctgtcacat acactcccac tatagttcca agttctgcat 5040 cagcatatgt ttcagaggaa gaagcagtta ccctaatagg aaatccttgg ccagatgacc 5100 tgttgtctac caaagaaagc tgggtagaag caactcctag acaagttgta gagctctcag 5160 ggagttcttc gattccaatt acagaaggct ctggagaagc agaagaagat gaagatacaa 5220 tgttcaccat ggtaactgat ttatcacaga gaaatactac tgatacactc attactttag 5280 acactagcag gataatcaca gaaagctttt ttgaggttcc tgcaaccacc atttatccag 5340 tttctgaaca accttctgca aaagtggtgc ctaccaagtt tgtaagtgaa acagacactt 5400 ctgagtggat ttccagtacc actgttgagg aaaagaaaag gaaggaggag gagggaacta 5460 caggtacggc ttctacattt gaggtatatt catctacaca gagatcggat caattaattt 5520 taccctttga attagaaagt ccaaatgtag ctacatctag tgattcaggt accaggaaaa 5580 gttttatgtc cttgacaaca ccaacacagt ctgaaaggga aatgacagat tctactcctg 5640 tctttacaga aacaaataca ttagaaaatt tgggggcaca gaccactgag cacagcagta 5700 • 40 ·

95238.doc 1327645 tccatcaacc tggggttcag gaagggctga ccactctccc acgtagtcct gcctctgtct 5760 ttatggagca gggctctgga gaagctgctg ccgacccaga aaccaccact gtttcttcat 5820 tttcattaaa cgtagagtat gcaattcaag ccgaaaagga agtagctggc actttgtctc 5880 cgcatgtgga aactacattc tccactgagc caacaggact ggttttgagt acagtaatgg 5940 acagagtagt tgctgaaaat ataacccaaa catccaggga aatagtgatt tcagagcgat 6000 taggagaacc aaattatggg gcagaaataa ggggcttttc cacaggtttt cctttggagg 6060 aagatttcag tggtgacttt agagaatact caacagtgtc tcatcccata gcaaaagaag 6120 aaacggtaat gatggaaggc tctggagatg cagcatttag ggacacccag acttcaccat 6180 ctacagtacc tacttcagtt cacatcagtc acatatctga ctcagaagga cccagtagca 6240 ccatggtcag cacttcagcc ttcccctggg aagagtttac atcctcagct gagggctcag 6300 gtgagcaact ggtcacagtr agcagctctg ttgttccagt gcttcccagt gctgtgcaaa 6360 agttttctgg lacagcttcc tccattatcg acgaaggatt gggagaagtg ggtactgtca 6420 atgaaattga tagaagatcc accattttac caacagcaga agtggaaggt acgaaagctc 6480 cagtagagaa ggaggaagta aaggtcagtg gcacagtttc aacaaacttt Cccc aaacta 6540 tagagccagc caaattatgg tctaggcaag aagtcaaccc tgtaagacaa gaaattgaaa 6600 gtgaaacaac atcagaggaa caaattcaag aagaaaagtc atttgaatcc cctcaaaact 6660 ctcctgcaac agaacaaaca atctttgatt cacagacatt tactgaaact gaactcaaaa 6720 ccacagatta ttctgtacta acaacaaaga aaacttacag tgatgataaa gaaatgaagg 6780 aggaagacac ttctttagtt aacatgtcta ctccagatcc agatgcaaat ggcttggaat 6840 cttacacaac tctccctgaa gctactgaaa agtcacattt tttcttagct actgcattag 6900 taactgaatc tataccagct gaacatgtag tcacagattc accaatcaaa aaggaagaaa 6960 gtacaaaaca ttttccgaaa ggcatgagac caacaattca agagtcagat actgagctct 7020 tattctctgg actgggatca ggagaagaag ttttacctac tctaccaaca gagtcagtga 7080 attttactga agtggaacaa atcaataaca cattatatcc ccacacttct caagtggaaa 7140 gtacctcaag tgacaaaatt gaagacttta acagaatgga aaatgtggca aaagaagttg 7200 gaccactcgt atctcaaaca gacatctttg aaggtagtgg gtcagtaacc agcacaacat 7260 taatagaaat tttaagtgac actggagcag aaggacccac ggtggcacct ctccctttct 7320 • 41 -

95238.doc 1327645 ccacggacat cggacatcct caaaatcaga ctgtcaggtg ggcagaagaa atccagacta 7380 gtagaccaca aaccataact gaacaagact ctaacaagaa ttcttcaaca gcagaaatta 7440 acgaaacaac aacctcatct actgattttc tggctagagc ttatggtttt gaaatggcca 7500 aagaatttgt tacatcagca ccaaaaccat ctgacttgta ttatgaacct tctggagaag 7560 gatctggaga agtggatatt gttgattcat ttcacacttc tgcaactact caggcaacca 7620 gacaagaaag cagcaccaca tttgtttctg atgggtccct ggaaaaacat cctgaggtgc 7680 caagcgctaa agctgttact gctgatggat tcccaacagt ttcagtgatg ctgcctcttc 7740 attcagagca gaacaaaagc tcccctgatc caactagcac actgtcaaat acagtgtcat 7800 atgagaggtc cacagacggt agtttccaag accgtttcag ggaattcgag gattccacct 7860 taaaacctaa cagaaaaaaa cccactgaaa atattatcat agacctggac aaagaggaca 7920 aggatttaat attgacaatt acagagagta ccatccttga aattctacct gagctgacat 7980 cggataaaaa tactatcata gatattgatc atactaaacc tgtgtatgaa gacattcttg 8040 gaatgcaaac agatatagat acagaggtac catcagaacc acatgacagt aatgatgaaa 8100 gtaatgatga cagcactcaa gttcaagaga tctatgaggc agctgtcaac Cttt ctttaa 8160 ctgaggaaac atttgagggc tctgctgatg ttctggctag ctacactcag gcaacacatg 8220 atgaatcaat gacttatgaa gatagaagcc aactagatca catgggcttt cacttcacaa 8280 ctgggatccc tgctcctagc acagaaacag aattagacgt tttacttccc acggcaacat 8340 ccctgccaat tcctcgtaag tctgccacag ttattccaga gattgaagga ataaaagctg 8400 aagcaaaagc cctggatgac atgtttgaat caagcacttt gtctgatggt caagctattg 8460 cagaccaaag tgaaataata ccaacattgg gccaatttga aaggactcag gaggagtatg 8520 aagacaaaaa acatgctggt ccttcttttc agccagaatt ctcttcagga gctgaggagg 8580 cattagtaga ccatactccc tatctaagta ttgctactac ccaccttatg gatcagagtg 8640 taacagaggt gcctgatgtg atggaaggat ccaatccccc atattacact gatacaacat 8700 tagcagtttc aacatttgcg aagttgtctt ctcagacacc atcatctccc ctcactatct 8760 actcaggcag tgaagcctct ggacacacag agatccccca gcccagtgct ctgccaggaa 8820 tagacgtcgg ctcatctgta atgtccccac aggattcttt taaggaaatt catgtaaata 8880 ttgaagcaac tttcaaacca tcaagtgagg aataccttca cataactgag cctccctctt 8940 -42-

95238.doc 1327645 tatctcctga cacaaaatta gaaccttcag aagatgatgg taaacctgag ttattagaag 9000 aaatggaagc ttctcccaca gaacttattg ctgtggaagg aactgagatt ctccaagatt 9060 tccaaaacaa aaccgatggt caagtttctg gagaa £ caat caagatgttt cccaccatta 9120 aaacacctga ggctggaact gttattacaa ctgccgatga aattgaatta gaaggtgcta 9180 cacagtggcc acactctact tctgcttctg ccacctatgg ggtcgaggca ggtgtggtgc 9240 cttggctaag tccacagact tctgagaggc ccacgcttic ttctictcca gaaataaacc 9300 ctgaaactca agcagcttta atcagagggc aggattccac gatagcagca tcagaacagc 9360 aagtggcagc gagaattctt gattccaatg atcaggcaac agtaaaccct gtggaattta 9420 atactgaggt tgcaacacca ccattttccc ttctggagac ttctaatgaa acagatttcc 9480 tgattggcat taatgaagag tcagtggaag gcacggcaat ctatttacca ggacctgatc 9540 gctgcaaaat gaacccgtgc cttaacggag gcacctgtta tcctactgaa acttcctacg 9600 tatgcacctg tgtgccagga tacagcggag accagtgtga acttgatttt gatgaatgtc 9660 actctaatcc ctgtcgtaat ggagccactt gtgttgatgg ttttaacaca ttcaggtgcc 9720 tctgccttcc aagttatgtt ggtgcacttt Gtgagcaaga taccgagaca tgt gactatg 9780 gctggcacaa attccaaggg cagtgctaca aatactttgc ccatcgacgc acatgggatg 9840 cagctgaacg ggaatgccgt ctgcagggtg cccatctcac aagcatcctg tctcacgaag 9900 aacaaatgtt tgttaatcgt gtgggccatg attatcagtg gataggcctc aatgacaaga 9960 tgtttgagca tgacttccgt tggactgatg gcagcacact gcaatacgag aattggagac 10020 ccaaccagcc agacagcttc ttttctgctg gagaagactg tgttgtaatc atttggcatg 10080 agaatggcca gtggaatgat gttccctgca attaccatct cacctatacg tgcaagaaag 10140 gaacagttgc ttgcggccag ccccctgttg tagaaaatgc caagaccttt ggaaagatga 10200 aacctcgtta tgaaatcaac tccctgatta gataccactg caaagatggt ttcattcaac 10260 gtcaccttcc aactatccgg tgcttaggaa atggaagatg ggctatacct aaaattacct 10320 gcatgaaccc atctgcatac caaaggactt attctatgaa atactttaaa aattcctcat 10380 cagcaaagga caattcaata aatacatcca aacatgatca tcgttggagc cggaggtggc 10440 aggagtcgag gcgctgatcc ctaaaatggc gaacatgtgt tttcatcatt tcagccaaag 10500 tcctaacttc ctgtgccttt cctatcacct cgagaagtaa ttatcagttg gtttggattt 10560 -43 -

95238.doc 1327645 ttggaccacc gttcagtcat tttgggttgc cgtgctccca aaacatttta aatgaaagta 10620 ttggcattca aaaagacagc agacaaaatg aaagaaaatg agagcagaaa gtaagcattt 10680 ccagcctatc taatttcttt agttttctat ttgcctccag tgcagtccat ttcctaatgt 10740 ataccagcct actgtactat ttaaaatgct caatttcagc accgatggcc atgtaaataa 10800 gatgatttaa tgttgatttt aatcctgtat ataaaataaa aagtcacaat gagtttgggc 10860 atatttaatg atgattatgg agccttagag gtctttaatc attggttcgg ctgcttttat 10920 gtagtttagg ctggaaatgg tttcacttgc tctttgactg tcagcaagac tgaagatggc 10980 mtcctgga cagctagaaa acacaaaatc ttgtaggtca ttgcacctat ctcagccata 11040 ggtgcagttt gcttctacat gatgctaaag gctgcgaatg ggatcctgat ggaactaagg 11100 actccaatgt cgaactcttc tttgctgcat tcctttttct tcacttacaa gaaaggcctg 11160 aatggaggac ttttctgtaa ccagg 11185 < 210 > 86 < 211 > 2503 < 212 > DNA < 213 > human <400> 86 ggactttgaa atccaacccg gtcacctacc cgcgcggg tgtccacgga tggcacgaaa 60 gccaagcgag tccccctgcc gagctactcg cgtccgcctc ctcccaagct gagctctgct 120 ccgcccacct gagtccttcg ccagttagga ggaaacacag ccgcttaatg aactgctgca 180 tcgggctggg agagaaagct cgcgggtccc accgggcctc ctacccaagt ctcagcgcgc 240 ttttcaccga ggcctcaatt ctgggatttg gcagctttgc tgtgaaagcc caatggacag 300 aggactgcag aaaatcaacc tatcctcctt caggaccaac gtacagaggt gcagttccat 360 ggtacaccat aaatcttgac ttaccaccct acaaaagatg gcatgaattg atgcttgaca 420 aggcaccaat gctaaaggtt atagtgaatt ctctgaagaa tatgataaat acattcgtgc 480 caagtggaaa agttatgcag gtggtggatg aaaaattgcc tggxtactt ggcaactttc 540 ctggcccttt tgaagaggaa Atgaagggta ttgccgctgt tactgatata cctttaggag 600 agattatttc attcaatatt ttttatgaat tatttaccat ttgtacttca atagtagcag 660 aagacaaaaa aggtcatcta atacatggga gaaacatgga ttttggagta tttcttgggt 720 -44·

95238.doc 1327645 ggaacataaa taatgatacc tgggtcataa ctgagcaact aaaaccttta acagtgaatt 780 tggatttcca aagaaacaac aaaactgtct tcaaggcttc aagctttgct ggctatgtgg 840 gcatgttaac aggattcaaa ccaggactgt tcagtcttac actgaatgaa cgtttcagta 900 taaatggtgg ttatctgggt attctagaat ggattctggg aaagaaagat gccatgtgga 960 tagggttcct cactagaaca gttctggaaa atagcacaag ttatgaagaa gccaagaatt 1020 tattgaccaa gaccaagata ttggccccag cctactttat cctgggaggc aaccagtctg 1080 gggaaggttg tgtgattaca cgagacagaa aggaatcatt ggatgiatat gaactcgatg 1140 ctaagcaggg tagatggtat gtggtacaaa caaattatga ccgttggaaa catcccttct 1200 tccttgatga tcgcagaacg cctgcaaaga tgtgtctgaa ccgcaccagc caagagaata 1260 tctcatttga aaccatgtat gatgtcctgt caacaaaacc tgtcctcaac aagctgaccg 1320 tatacacaac cttgatagat gttaccaaag gtcaattcga aacttacctg cgggactgcc 1380 ctgacccttg tataggttgg tgagcacacg tctggcctac agaatgcggc ctctgagaca 1440 tgaagacacc atctccatgt gaccgaacac tgcagctgtc tgaccttcca aagactaaga 1500 ctcgcggcag gttctctttg agtcaaaagc ttgtcttcgt ccatctgttg Acaaatga ca 1560 gaccmttt tttcccccat cagttgattt ttcttattta cagataactt ctttagggga 1620 agtaaaacag tcatctagaa ttcactgagt tttgtttcac tttgacattt ggggatctgg 1680 tgggcagtcg aaccatggtg aactccacct ccgtggaata aatggagatt cagcgtgggt 1740 gttgaatcca gcacgtctgt gtgagtaacg ggacagtaaa cactccacat tcttcagttt 1800 ttcacttcta cctacatatt tgtatgtttt tctgtataac agccttttcc ttctggttct 1860 aactgctgtt aaaattaata tatcattatc tttgctgtta ttgacagcga tataatttta 1920 ttacatatga ttagagggat gagacagaca ttcacctgta tatttctttt aatgggcaca 1980 aaatgggccc ttgcctctaa atagcacttt ttggggttca agaagtaatc agtatgcaaa 2040 gcaatctttt atacaataat tgaagtgttc cctttttcat aattactgta cttcccagta 2100 accctaagga agttgctaac ttaaaaaact gcatcccacg ttctgttaat ttagtaaata 2160 aacaagtcaa agacttgtgg aaaataggaa gteaacccat attttaaatt ctcataagta 2220 gcattcatgt aataaacagg tttttagttt gttcttcaga ttgataggga gttttaaaga 2280 aattttagta gttactaaaa ttatgttact gtatttttca gaaatcaaac tgcttatgaa 2340 • 45 ·

。 。 。 。 。 。 。 。 。 。 。 。 。 ggctcttctt tgcctctgct ggagtccggg gagtggcgtt ggctgctaga gcgatgccgg 60 gccggagttg cgtcgcctta gtcctcctgg ctgccgccgt cagctgtgcc gtcgcgcagc 120 acgcgccgcc gtggacagag gactgcagaa aatcaaccta tcctccttca ggaccaacgt 180 acagaggtgc agttccatgg tacaccataa atcttgactt accaccctac aaaagatggc 240 atgaattgat gcttgacaag gcaccaatgc taaaggttat agtgaattct ctgaagaata 300 tgataaatac attcgtgcca agtggaaaag ttatgcaggt ggtggatgaa aaattgcctg 360 gcctacttgg caactttcct ggcccttttg aagaggaaat gaagggtatt gccgctgtta 420 ctgatatacc Tttaggagag attatttcat tcaatatttt ttatgaatta tttaccattt 480 gtacttcaat agtagcagaa gacaaaaaag gtcatctaat acatgggaga aacatggatt 540 ttggagtatt tcttgggtgg aacataaata atgatacctg ggtcataact gagcaactaa 600 aacctt taac agtgaatttg gatttccaaa gaaacaacaa aactgtcttc aaggcttcaa 660 gctttgctgg ctatgtgggc atgttaacag gattcaaacc aggactgttc agtcttacac 720 tgaatgaacg tttcagtata aatggtggtt atctgggtat tctagaatgg attctgggaa 780 agaaagatgc catgtggata gggttcctca ctagaacagt tctggaaaat agcacaagtt 840 atgaagaagc caagaattta ttgaccaaga ccaagatatt ggccccagcc tactttatcc 900 tg £ gaggcaa ccagtctggg gaaggttgtg tgattacacg agacagaaag gaatcattgg 960 atgtatatga actcgatgct aagcagggta gatggtatgt ggtacaaaca aattatgacc 1020 gttggaaaca tcccttcttc cttgatgatc gcagaacgcc tgcaaagatg tgtctgaacc 1080 gcaccagcca agagaatatc tcatttgaaa ccatgtatga tgtcctgtca acaaaacctg 1140 tcctcaacaa gctgaccgta tacacaacct tgatagatgt taccaaaggt caattcgaaa 1200 cttacctgcg ggactgccct gacccttgta taggttggtg agcacacgtc tggcctacag 1260 -46 ·

95238.doc 1327645 aatgcggcct ctgagacatg aagacaccat ctccatgtga ccgaacactg cagctgtctg 1320 accttccaaa gactaagact cgcggcaggt tctctttgag tcaaaagctt gtcttcgtcc 1380 atctgttgac aaatgacaga cctttttttt tcccccatca gttgattttt cttatttaca 1440 gataacttct ttaggggaag taaaacagtc atctagaatt cactgagttt tgtttcactt 1500 tgacatttgg ggatctggtg ggcagtcgaa ccatggtgaa ctccacctcc gtggaataaa 1560 tggagattca gcgtgggtgt tgaatccagc acgtctgtgt gagtaacggg acagtaaaca 1620 ctccacattc ttcagttttt cacttctacc tacatatttg tatgtttttc tgtataacag 1680 ccttttcctt ctggttctaa ctgctgttaa aattaatata tcattatctt tgctgttatt 1740 gacagcgata taattttatt acatatgatt agagggatga gacagacatt cacctgtata 1800 tttcttttaa tgggcacaaa atgggccctt gcctctaaat agcacttttt ggggttcaag 1860 aagtaatcag tatgcaaagc aatcttttat acaataattg aagtgttccc tttttcataa 1920 ttactgtact tcccagtaac cctaaggaag ttgctaactt aaaaaactgc atcccacgtt 1980 ctgttaattt agtaaataaa caagtcaaag acttgtggaa aataggaagt gaacccatat 2040 tttaaattct cataagtagc attcatgtaa taaacaggtt tttagtttgt Tctt cagatt 2100 gatagggagt tttaaagaaa ttttagtagt tactaaaatt atgttactgt atttttcaga 2160 aatcaaactg cttatgaaaa gtactaatag aacttgttaa cctttctaac cttcacgatt 2220 aactgtgaaa tgtacgtcat ttgtgcaaga ccgtttgtcc acttcatttt gtataatcac 2280 agttgtgttc ctgacactca ataaacagtc attggaaaga gtgccagtca gcagtcatgc 2340 a 2341 < 210 > 88 < 211> 2039 < 212 > DNA < 213 > human < 400 > 88 ccggccctcg ccctgtccgc cgccaccgcc gccgccgcca gagtcgccat gcagatcccg 60 cgcgccgctc ttctcccgct gctgctgctg ctgctggcgg cgcccgcctc ggcgcagctg 120 tcccgggccg gccgctcggc gcctttggcc gccgggtgcc cagaccgctg cgagccggcg 180 cgctgcccgc cgcagccgga gcactgcgag ggcggccggg cccgggacgc gtgcggctgc 240 • 47-

95238.doc 1327645 tgcgaggtgt gcggc £ cgcc cgagggcgcc gcgtgcggcc tgcaggaggg cccgtgcggc 300 gaggggctgc agtgcgtggt gcccttcggg gtgccagcct cggccacggt gcggcggcgc 360 gcgcaggccg gcctctgtgt gtgcgccagc agcgagccgg tgtgcggcag cgacgccaac 420 acctacgcca acctgtgcca gctgcgcgcc gccagccgcc gctccgagag gctgcaccgg 480 ccgccggtca tcgtcctgca gcgcggagcc tgcggccaag ggcaggaaga tcccaacagt 540 ttgcgccata aatataactt tatcgcggac gtggtggaga agatcgcccc tgccgtggtt 600 catatcgaai tgittcgcaa gcttccgttt tctaaacgag aggtgccggt ggctagtggg 660 tctgggttta ttgtgtcgga agatggactg atcgtgacaa atgcccacgt ggtgaccaac 720 aagcaccggg tcaaagttga gctgaagaac ggtgccactt acgaagccaa aatcaaggat 780 gtggatgaga aagcagacat cgcactcatc aaaattgacc accagggcaa gctgcctgtc 840 ctgctgcttg gccgctcctc agagctgcgg ccgggagagt tcgtggtcgc catcggaagc 900 ccgttttccc ttcaaaacac agtcaccacc gggatcgtga gcaccaccca gcgaggcggc 960 aaagagctgg ggctccgcaa ctcagacatg gactacatcc agaccgacgc catcatcaac 1020 tatggaaact cgggaggccc gttagtaaac Ctggacggtg aagt£attgg aattaacact 108 0 ttgaaagtga cagctggaat ctcctttgca atcccatctg ataagattaa aaagttcctc 1140 acggagtccc atgaccgaca ggccaaagga aaagccatca ccaagaagaa gtatattggt 1200 atccgaatga tgtcactcac gtccagcaaa gccaaagagc tgaaggaccg gcaccgggac 1260 ttcccagacg tgatctcagg agcgtatata attgaagtaa ttcctgatac cccagcagaa 1320 gctggtggtc tcaaggaaaa cgacgtcata atcagcatca atggacagtc cgtggtctcc 1380 gccaatgatg tcagcgacgt cattaaaagg gaaagcaccc tgaacatggt ggtccgcagg 1440 ggtaatgaag atatcatgat cacagtgatt cccgaagaaa ttgacccata ggcagaggca 1500 tgagctg ^ ac ttcatgtttc cctcaaagac tctcccgtgg atgacggatg aggactctgg 1560 gctgctggaa taggacactc aagacttttg actgccattt tgtttgttca gtggagactc 1620 cctggccaac agaatccttc ttgatagttt gcaggcaaaa caaatgtaat gttgcagatc 1680 cgcaggcaga agctctgccc ttctgtatcc tatgtatgca gtgtgctttt tcttgccagc 1740 ttgggccatt cttgcttaga cagtcagcat ttgtctcctc ctttaactga gtcatcatct 1800 tagtccaact aatgcagtcg aiacaatgcg tagatagaag aagccccacg ggagccagga 1860 • 48- 95238.doc 1327645 tgggactggt cgtgtttgtg cttttctcca agtcagcacc caaagg tcaa tgcacagaga 1920 ccccgggtgg gtga £ cgctg gcttctcaaa tgcctctttt aggaatctct 1980 ttggaattgg gagcacgatg actctgagtt tgagctatta aagtacttct tacacattg 2039 & lt cggccgaagt; 210 > 89 < 211 > 1387 < 212 > DNA < 213> Human < 400 > 89 ccgggtcgga gccccccgga gctgcgcgcg ggcttgcagc gcctcgcccg cgctgtcctc 60 ccggtgtccc gcttctccgc gccccagccg ccggctgcca gcttttcggg gccccgagtc 120 gcacccagcg aagagagcgg gcccgggaca agctcgaact ccggccgcct cgcccttccc 180 cggctccgct ccctctgccc cctcggggtc gcgcgcccac gatgctgcag ggccctggct 240 cgctgctgct gctcttcctc gcctcgcact gctgcctggg ctcggcgcgc gggctcttcc 300 tctttggcca gcccgacttc tcctacaagc gcagcaattg caagcccatc cctgccaacc 360 tgcagctgtg ccacggcatc gaataccaga acatgcggct gcccaacctg ctgggccacg 420 agaccatgaa ggaggtgctg gagcaggccg gcgcttggat cccgctggtc atgaagcagt 480 Gccacccgga caccaagaag ttcctgtgct cgctcttcgc ccccgtctgc ctcgatgacc 540 tagacgagac catccagcca tgccactcgc tctgcgtgca ggtgaaggac cgctgcgccc 600 cggtcatgtc cgccttcggc ttcccctggc ccgacatgct tgagtgcgac cgt ttccccc 660 aggacaacga cctttgcatc cccctcgcta gcagcgacca cctcctgcca gccaccgagg 720 aagctccaaa ggtatgtgaa gcctgcaaaa ataaaaatga tgatgacaac gacataatgg 780 aaacgctttg taaaaatgat tttgcactga aaataaaagt gaaggagata acctacatca 840 accgagatac caaaatcatc ctggagacca agagcaagac catttacaag ctgaacggtg 900 tgtccgaaag ggacctgaag aaatcggtgc tgtggctcaa agacagcttg cagtgcacct 960 gtgaggagat gaacgacatc aacgcgccct atctggtcat gggacagaaa cagggtgggg 1020 agctggtgat cacctcggtg aagcggtggc agaaggggca gagagagttc aagcgcatct 1080 cccgcagcat ccgcaagctg cagtgctagt cccggcatcc tgatggctcc gacaggcctg 1140 ctccagagca cggctgacca tttctgctcc gggatctcag ctcccgttcc ccaagcacac 1200 tcctagctgc tccagtctca gcctgggcag cttccccctg ccttttgcac gtttgcatcc 1260 -49·

95238.doc 1327645 ccagcatttc ctgagttata aggccacagg agtggatagc tgttttcacc taaaggaaaa 1320 gcccacccga atcttgtaga aatattcaaa ctaataaaat catgaatatt tttatgaagt 1380 ttaaaaa 1387 < 210 > 90 < 211 > 1092 < 212 > DNA < 213 > human < 400 > 90 tgtccctgga attctgggac actggctggg gtttgaggag agaagccagt acctacctgg 60 ctgcaggatg aagctggcca gtggcttctt ggttttgtgg ctcagccttg ggggtggcct 120 ggctcagagc gacacgagcc ctgacacgga ggagtcctat tcagactggg gccttcggca 180 cctccgggga agctttgaat ccgtcaatag ctacttcgat tcttttctgg agctgctggg 240 agggaagaat ggagtctgtc agtacaggtg ccgatatgga aaggcaccaa tgcccagacc 300 tggctacaag ccccaagagc ccaatggctg cggctcctat ttcctgggtc tcaaggtacc 360 agaaagtatg gacttgggca ttccagcaat gacaaagtgc tgcaaccagc tggatgtctg 420 ttatgacact tgcggtgcca acaaatatcg ctgtgatgca aaattccgat ggtgtctcca 480 ctcgatctgc tctgacctta agcggagtct gggctttgtc tccaaagtgg aagcagcctg 540 tgattccctg gttgacactg tgttcaacac cgtgtggacc ttgggctgcc gcccctttat 600 gaatagtcag cgggcagctt gcatctgtgc agaggaggag aag gaagagt tatgaggaag 660 aagtgaucc ttcctggttt tgagtgacac cacagctgtc agccttcaag atgtcaagtc 720 ttcgagtcag cgtgactcat tcattcttcc aacagtttgg acaccacaaa gcaggagaaa 780 gggaacattt ttctacagct ggaaagtgag tcctatcctt tgaggaaatt tgaaaaaaga 840 catggagtgg tttgaaagct actcttcatt taagactgct ctccccaacc aagacacatt 900 tgcctggaaa ttcagttctt agcttaaaga ctaaaatgca agcaaaccct gcaattcctg 960 gacctgatag ttatattcat gagtgaaatt gtggggagtc cagccatttg ggaggcaatg 1020 actttctgct ggcccatgtt tcagttgcca gtaagcttct cacatttaat Aaagtgtact 1080 ttttagaaca tt 1092 <210> 91 50 -

95238.doc 1327645 < 211 > 1807 < 212 > DNA < 213 > human < 400 > 91 gcacgaggga agagggtgat ccgacccggg gaaggtcgct gggcagggcg agttgggaaa 60 gcggcagccc ccgccgcccc cgcagcccct tctcctcctt tctcccacgt cctatctgcc 120 tctcgctgga ggccaggccg tgcagcatcg aagacaggag gaactggagc ctcattggcc 180 ggcccggggc gccggcctcg ggcttaaata ggagctccgg gctctggctg ggacccgacc 240 gctgccggcc gcgctcccgc tgctcctgcc gggtgatgga aaaccccagc ccggccgccg 300 ccctgggcaa ggccctctgc gctctcctcc tggccactct cggcgccgcc ggccagcctc 360 ttgggggaga gtccatctgt tccgccagag ccccggccaa atacagcatc accttcacgg 420 gcaagtggag ccagacggcc ttccccaagc agtaccccct gttccgcccc cctgcgcagt 480 ggtcttcgct gctgggggcc gcgcatagct ccgactacag catgtggagg aagaaccagt 540 acgtcagtaa cgggctgcgc gactttgcgg agcgcggcga ggcctgggcg ctgatgaagg 600 agatcgaggc ggcgggggag gcgctgcaga gcgtgcacgc ggtgttttcg gcgcccgccg 660 tccccagcgg caccgggcag acgtcggcgg agctggaggt gcagcgcagg cactcgctgg 720 tctcgtttgt ggtgcgcatc gtgcccagcc ccgactggtt cgtgggcgtg gacagcctgg 780 acctgtg cga cggggaccgt tggcgggaac aggcggcgct ggacctgtac ccctacgacg 840 ccgggacgga cagcggcttc accttctcct cccccaactt cgccaccatc ccgcaggaca 900 cggtgaccga gataacgtcc tcctctccca gccacccggc caactccttc tactacccgc 960 ggctgaaggc cctgcctccc atcgccaggg tgacactggt gcggctgcga cagagcccca 1020 gggccttcat ccctcccgcc ccagtcctgc ccagcaggga caatgagatt gtagacagcg 1080 cctcagttcc agaaacgccg ctggactgcg aggtctccct gtggtcgtcc tggggactgt 1140 gcggaggcca ctgtgggagg ctcgggacca agagcaggac tcgctacgtc cgggtccagc 1200 ccgccaacaa cgggagcccc tgccccgagc tcgaagaaga ggctgagtgc gtccctgata 1260 actgcgtcta agaccagagc cccgcagccc ctggggcccc cggagccatg gggtgtcggg 1320 ggctcctgtg caggctcatg ctgcaggcgg ccgaggcaca gggggtttcg cgctgctcct 1380 gaccgcggtg aggccgcgcc gaccatctct gcactgaagg gccctctggt ggccggcacg 1440 ggcattggga aacagcctcc tcctttccca accttgcttc ttaggggccc ccgtgtcccg 1500 -51 -

95238.doc 1327645 tctgctctca gcctcctcct cctgcaggat aaagtcatcc ccaaggctcc agctactcta 1560 aattatggtc tccttataag ttattgctgc tccaggagat tgtccttcat cgtccagggg 1620 cctggctccc acgtggttgc agatacctca gacctggtgc tctaggctgt gctgagccca 1680 ctctcccgag ggcgcatcca agcgggggcc acttgagaag tgaataaatg gggcggtttc 1740 ggaagcgtca gtgtttccat gttatggatc tctctgcgtt tgaataaaga ctatctctgt 1800 tgctcac 1807 < 210 > 92 < 211 > 1077 < 212 > DNA < 213 > human < 400 > 92 cccgcccccg ccccttccga gcaaactttt ggcacccacc gcagcccagc gcgcgttcgt 60 gctccgcagg gcgcgcctct ctccgccaat gccaggcgcg cgggggagcc attaggaggc 120 gaggagagag gagggcgcag ctcccgccca gcccagccct gcccagccct gcccggaggc 180 agacgcgccg gaaccgggac gcgataaata tgcagagcgg aggcttcgcg cagcagagcc 240 cgcgcgccgc ccgctccggg tgctgaatcc aggcgtgggg acacgagcca ggcgccgccg 300 ccggagccag cggagccggg gccagagccg gagcgcgtcc gcgtccacgc agccgccggc 360 cggccagcac ccagggccct gcatgccagg tcgttggagg tggcagcgag acatgcaccc 420 ggcccggaag ctcctcagcc tcctcttcct catcctgatg ggcactgaac tcactcaaaa 480 taaaagagaa aacaaagcag agaagatggg agggccagag agcgagagga agaccacagg 540 agagaagaca ctgaacgagc ttcccttgtt ttgcctg £ aa gcccacgctg gctccctggc 600 tctgcccagg atgtgcagtc caaatcccaa tccagcagtg gggttatgtc gtcccgctta 660 ccctcagagc ccttctcctg gtgctgccca gacgatcagc cagtccctcc tggagaggtt 720 ctgcatggcc tctaggagag aagttttctt ggccccagga aggcctggtg gagggtggtg 780 gttgtgcact gttgctggac agatgcattc attcatgtgc acacacacac acacacatgc 840 acacaca £ gg Gagcagatac ctgcagagaa gagccaacca ggtcctgatt agtggcaagc 900 tgccccacaa agggctatgc ctgtgtctta ttgagacacc ttggcaaaga gatg£ctgat 960 tctgggtggt cctggacatg gccgcaccca agggccctcc aagccttaat ggcaccctga 1020 -52·

95238.doc 1327645 agcctccatg cccaggccaa aagatgcttt tcctccctaa aaaaaaaaaa aaaaaaa 1077 < 210 > 93 < 211 > 4229 < 212 > DNA < 213 > human < 400 > 93 ggggccccag tggccgccgc ggagcgaggt tgcctggaga gagcgcctgg gcgcagaagg 60 gttaacgggc caccg £ gggc tcgcagagca ggagggtgct ctcggacggt gtgtccccca 120 ctgcactcct gaacttggag gacagggtc £ ccgcgaggga cgcagagagc accctccacg 180 cccagatgcc tgcgtagttt ttgtgaccag tccgctcctg cctccccctg gggcagtaga 240 gggggagcga tggagaactg gactggcagg ccctggctgt atctgctgct gcttctgtcc 300 ctccctcagc tctgcttgga tcaggaggtg ttgtccggac actctcttca gacacctaca 300 gaggagggcc agggccccga aggtgtctgg ggaccttggg tccagtgggc ctcttgctcc 420 cagccctgcg gggtgggggt gcagcgcagg agccggacat gtcagctccc tacagtgcag 480 ctccacccga gtctgcccct ccctccccgg cccccaagac atccagaagc Cctcctcccc 540 cggggccagg gtcccagacc ccagacttct ccagaaaccc tccccttgta caggacacag 600 tctcggggaa ggggtggccc acttcgaggt cccgcttccc acctagggag agaggagacc 660 caggagattc gagcggccag gaggtcccgg cttcgagacc ccatcaagcc aggaatg ttc 720 ggttatggga gagtgccctt tgcattgcca ctgcaccgga accgcaggca ccctcggagc 780 ccacccagat ctgagctgtc cctgatctct tctagagggg aagaggctat tccgtcccct 840 actccaagag cagagccatt ctccgcaaac ggcagccccc aaactgagct ccctcccaca 900 gaactgtctg tccacacccc atccccccaa gcagaacctc taagccctga aactgctcag 960 acagaggtgg cccccagaac caggcctgcc cccctacggc atcaccccag agcccaggcc 1020 tctggcacag agcccccctc acccacgcac tccttaggag aaggtggctt cttccgtgca 1080 tcccctcagc cacgaaggcc aagttcccag ggttgggcca gtccccaggt agcagggaga 1140 cgccctgatc cttttccttc ggtccctcgg ggccgaggcc agcagggcca agggccttgg 1200 ggaacggggg ggactcctca cgggccccgc ctggagcctg accctcagca cccgggcgcc 1260 tggctgcccc tgctgagcaa cggcccccat gccagctccc tctggagcct ctttgctccc 1320 agtagcccta ttccaagatg ttctggggag agtgaacagc taagagcctg cagccaagcg 1380 -53-

95238.doc 1327645 ccctgccccc ctgagcagcc agacccccgg gccctgcagt gcgcagcctt taactcccag 1440 gaattcatgg gccagctgta tcagtgggag cccttcactg aagtccaggg ctcccagcgc 1500 tgtgaactga actgccggcc ccgtggcttc cgcttctatg tccgtcacac tgaaaaggtx: 1560 caggatggga ccctgtgtca gcctggagcc cctgacatct gtgtggctgg acgctgtctg 1620 agccccggct gtgatgggat ccttggctct ggcaggcgtc ctgatggctg tggagtctgt 1680 £ g £ ggtgatg attctacctg tcgccttgtt tcggggaacc tcactgaccg agggggcccc 1740 ctgggctatc agaagatctt gtggattcca gcgggagcct tgcggctcca gattgcccag 1800 ctccggccta gctccaacta cctggcactt cgtggccctg ggggccggtc catcatcaat 1860 gggaactggg ctgtggatcc ccctgggtcc tacagggccg gcgggaccgt ctttcgatat 1920 aaccgtcctc ccagggagga gggcaaaggg gagagtctgt cggctgaagg cxccaccacc 1980 cagcctgtgg atgtctatat gatctttcag gaggaaaacc caggcgtttt ttatcagtat 2040 gtcatctctt cacctcctcc aatccttgag aaccccaccc cagagccccc tgtcccccag 2100 cttcagccgg agattctgag ggtggagccc ccacttgctc cggcaccccg cccagcccgg 2160 accccaggca Ccctccagcg tcaggtgcgg atcccccaga tgcccgcccc g ccccatccc 2220 aggacacccc tggggtctcc agctgcgtac tggaaacgag tgggacactc tgcatgctca 2280 gcgtcctgcg ggaaaggtgt ctggcgcccc attttcctct gcatctcccg tgagtcggga 2340 gaggaactgg atgaacgcag ctgtgccgcg ggtgccaggc ccccagcctc ccctgaaccc 2400 tgccacggca ccccatgccc cccatactgg gaggctggcg agtggacatc ctgcagccgc 2460 tcctgtggcc ccggcaccca gcaccgccag ctgcagtgcc ggcaggaatt tggggggggt 2520 ggctcctcgg tgcccccgga gcgctgtgga catctccccc ggcccaacat cacccagtct 2580 tgccagctgc gcctctgtgg ccattgggaa gttggctctc cttggagcca gtgctccgtg 2640 cggtgcggcc ggggccagag aagccggcag gttcgctgtg ttgggaacaa cggtgatgaa 2700 gtgagcgagc aggagtgtgc gtcaggcccc ccgcagcccc ccagcagaga ggcctgtgac 2760 atggggccct gtactactgc ctggttccac agcgactgga gctccaagtg ctcagccgag 2820 tgtgggacgg gaatccagcg gcgctctgtg gtctgccttg ggagtggggc agccctcggg 2880 ccaggccagg gggaagcagg agcaggaact gggcagagct gtccaacagg aagccggccc 2940 cctgacatgc gcgcctgcag cctggggccc tgtgagagaa cttggcgctg gtacacaggg 3000 • 54 ·

95238.doc 1327645 ccctggggtg agtgctcctc cgaatgtggc tctggcacac agcgtagaga catcatctgt 3060 gtatccaaac tggggacgga gttcaacgtg acttctccga gcaactgttc tcacctcccc 3120 aggccccctg ccctgcagcc ctgtcaaggg caggcctgcc aggaccgatg gttttccacg 3180 ccctggagcc catgttctcg ctcctgccaa gggggaacgc agacacggga ggtccagtgc 3240 ctgagcacca accagaccct cagcacccga tgccctcctc aactgcggcc ctccaggaag 3300 cgcccctgta acagccaacc ctgcagccag cgccctgatg atcaatgcaa ggacagctct 3360 ccacattgcc ccctggtggt acaggcccgg ctctgcgtct acccctacta cacagccacc 3420 tgttgccgct cttgcgcaca tgtcctggag cggtctcccc aggatccctc ctgaaagggg 3480 tccggggcac cttcacggtt ttctgtgcca ccatcggtca cccattgatc ggcccactct 3540 gaaccccctg gctctccagc ctgtcccagt ctcagcaggg atgtcctcca ggtgacagag 3600 ggtggcaagg tgactgacac aaagtgactt tcagggctgt ggtcaggccc atgtggtggt 3660 gtgatgggtg tgtgcacata tgcctcaggt gtgcttttgg gactgcatgg atatgtgtgt 3720 gctcaaacgt gtatcacttt tcaaaaagag gttacacaga ctgagaagga caagacctgt 3780 ttccttgaga ctttcctagg tggaaaggaa agcaagtctg cagttccttg Ctaa tctgag 3840 ctacttagag tgtggtctcc ccaccaactc cagttttgtg ccctaagcct catttctcat 3900 gttcagacct cacatcttct aagccgccct gtgtctctga ccccttctca tttgcctagt 3960 atctctgccc ctgcctccct aattagctag ggctggggtc agccactgcc aatcctgcct 4020 tactcaggaa ggcaggagga aagagactgc ctctccagag caaggcccag ctgggcagag 4080 ggtgaaaaag agaaatgtga gcatccgctc ccccaccacc ccgcccagcc cctagcccca 4140 ctccctgcct cctgaaatgg ttcccaccca gaactaattt attttttatt aaagatggtc 4200 atgacaaatg aaaaaaaaaa aaaaaaaaa 4229 <210> 94 <211> 5826 <212> DNA <213>human<400> 94 gaggaggaga cggcatccag tacagagggg ctggacttgg acccctgcag cagccctgca 60 caggagaagc ggcatataaa gccgcgctgc ccgggagccg ctcggccacg tccaccggag 120 -55-

95238.doc 1327645 catcctgcac tgcagggccg gtctctcgct ccagcagagc ctgcgccttt ctgactcggt 180 ccggaacact gaaaccagtc atcactgcat ctttttggca aaccaggagc tcagctgcag 240 gaggcaggat ggtctggagg ctggtcctgc tggctctgtg ggtgtggccc agcacgcaag 300 ctggtcacca ggacaaagac acgaccttcg accttttcag tatcagcaac atcaaccgca 360 agaccattgg cgccaagcag ttccgcgggc ccgaccccgg cgtgccggct taccgcttcg 420 tgcgctttga ctacatccca ccggtgaacg cagatgacct cagcaagatc accaagatca 480 tgcggcagaa ggagggcttc ttcctcacgg cccagctcaa gcaggacggc aagtccaggg 540 gcacgctgtt ggctctggag ggccccggtc tctcccagag gcagttcgag atcgtctcca 600 acggccccgc ggacacgctg gatctcacct actggattga cggcacccgg catgtggtct 660 ccctggagga cgtcggcctg gctgactcgc agtggaagaa cgtcaccgtg caggtggctg 720 gcgagaccta cagcttgcac gtgggctgcg acctcataga cagcttcgct ctggacgagc 780 ccttctacga gcacctgcag gcggaaaaga gccggatgta cgtggccaaa ggctctgcca 840 gagagagtca cttcaggggt ttgcttcaga acgtccacct agtgtttgaa aactctgtgg 900 aagatattct aagcaagaag ggttgccagc aaggccaggg agctgagatc Aacgccatca 960 gt gagaacac agagacgctg cgcctgggtc cgcatgtcac caccgagtac gtgggcccca 1020 gctcggagag gaggcccgag gtgtgcgaac gctcgtgcga ggagctggga aacatggtcc 1080 aggagctctc ggggctccac gtcctcgtga accagctcag cgagaacctc aagagagtgt 1140 cgaatgataa ccagtttctc tgggagctca ttggtggccc tcctaagaca aggaacatgt 1200 cagcttgctg gcaggatggc cggttctttg cggaaaatga aacgtgggtg gtggacagct 1260 gcaccacgtg tacctgcaag aaatttaaaa ccatttgcca ccaaatcacc tgcccgcctg 1320 caacctgcgc cagtccatcc tttgtggaag gcgaatgctg cccttcctgc ctccactcgg 1380 tggacggtga ggagggctgg tctccgtggg cagagtggac ccagtgctcc gtgacgtgtg 1440 gctctgggac ccagcagaga ggccggtcct gtgacgtcac cagcaacacc tgcttggggc 1500 cctccatcca gacacgggct tgcagtctga gcaagtgtga cacccgcatc cggcaggacg 1560 gcggctggag ccactggtca ccttggtctt catgctctgt gacctgtgga gttggcaata 1620 tcacacgcat ccgtctctgc aactccccag tgccccagat ggggggcaag aattgcaaag 1680 giagtggccg ggagaccaaa gcctgccagg gcgccccatg cccaatcgat ggccgctgga 1740 -56-

95238.doc 1327645 gcccctggtc cccgtggtcg gcctgcactg tcacctgtgc cggtgggatc cgggagcgca 1800 cccgggtctg caacagccct gagcctcagt acggagggaa ggcctgcgtg ggggatgtgc 1860 aggagcgtca gatgtgcaac aagaggagct gccccgtgga tggctgttta tccaacccct 1920 gcttcccggg agcccagtgc agcagcttcc ccgatgggtc ctggtcatgc ggctcctgcc 1980 ctgtgggctt cttgggcaat ggcacccact gtgaggacct ggacgagtgt gccctggtcc 2040 ccgacatctg cttctccacc agcaaggtgc ctcgctgtgt caacactcag cctggcttcc 2100 acigcctgcc ctgcccgccc cgaiacagag ggaaccagcc cgtcggggtc ggcctggaag 2160 cagccaagac ggaaaagcaa gtgtgtgagc ccgaaaaccc atgcaaggac aagacacaca 2220 actgccacaa gcacgcggag tgcatctacc tgggccactt cagcgacccc atgtacaagt 2280 gcgagtgcca gacaggctac gcgggcgacg ggctcatctg cggggaggac tcggacctgg 2340 acggctggcc caacctcaat ctggtctgcg ccaccaacgc cacctaccac tgcalcaagg 2400 ataactgccc ccatctgcca aattctgggc aggaagactt tgacaaggac gggattggcg 2460 atgcctgtga tgatgacgat gacaatgacg gtgtgaccga tgagaaggac aactgccagc 2520 tcctcttcaa tccccgccag gctgactatg acaaggatga ggttggggac Cgct gtgaca 2580 actgccctta cgtgcacaac cctgcccaga tcgacacaga caacaatgga gagggtgacg 2640 cctgctccgt ggacattgat ggggacgatg tcttcaatga acgagacaat tgtccctacg 2700 tctacaacac tgaccagagg gacacggatg gtgacggtgt gggggatcac tgtgacaact 2760 gccccctggt gcacaaccct gaccagaccg acgtggacaa tgaccttgtt ggggaccagt 2820 gtgacaacaa cgaggacata gatgacgacg gccaccagaa caaccaggac aactgcccct 2880 acatctccaa cgccaaccag gctgaccatg acagagacgg ccagggcgac gcctgtgacc 2940 ctgatgatga caacgatggc gtccccgatg acagggacaa ctgccggctt gtgttcaacc 3000 cagaccagga ggacttggac ggtgatggac ggggtgatat ttgtaaagat gattttgaca 3060 atgacaacat cccagatatt gatgatgtgt gtcctgaaaa caatgccatc agtgagacag 3120 acttcaggaa cttccagatg gtccccttgg atcccaaagg gaccacccaa attgatccca 3180 actgggtcat tcgccatcaa ggcaaggagc tggttcagac agccaactcg gaccccggca 3240 tcgctgtag £ ttttgacgag tttgggtctg tggacttcag tggcacattc tacgtaaaca 3300 ctgaccggga cgacgactat gccggcttcg tctttggtta ccagtcaagc agccgcttct 3360 • 57 ·

95238.doc 1327645 atgtggtgat gtggaagcag gtgacgcaga cctactggga ggaccagccc acgcgggcct 3420 atggctactc cggcgtgtcc ctcaaggtgg tgaactccac cacggggacg ggcgagcacc 3480 tgaggaacgc gctgtggcac acggggaaca cgccggggca ggtgcgaacc ttatggcacg 3540 accccaggaa cattggctgg aaggactaca cggcctatag gtggcacctg acicacaggc 3600 ccaagactgg ctacatcaga gtcttagtgc atgaaggaaa acaggtcatg gcagactcag 3660 gacctatcta tgaccaaacc tacgctggcg ggcggctggg tctatttglc ttctctcaag 3720 aaatggtcta tttctcagac ctcaagtacg aatgcagaga tatttaaaca agatttgctg 3780 catttccggc aatgccctgt gcatgccatg gtccctagac acctcagttc attgtggtcc 3840 ttgtggcttc tctctctagc agcacctcct gtcccttgac cttaactctg atggttcttc 3900 acctcctgcc agcaacccca aacccaagtg ccttcagagg ataaatatca atggaactca 3960 gagatgaaca tctaacccac tagaggaaac cagtttggt £ atatatgaga ctttatgtgg 4020 agtgaaaatt gggcatgcca ttacattgct ttttcttgtt tgtttaaaaa gaatgacgtt 4080 tacatataaa atgtaattac ttattgtatt tatgtgtata tggagttgaa gggaatactg 4140 tgcataagcc attatgataa attaagcatg aaaaatattg Ctgaactact ttt ggtgctt 4200 aaagttgtca ctattcttga attagagttg ctctacaatg acacacaaat cccattaaat 4260 aaattataaa caagggtcaa ttcaaatttg aagtaatgtt ttagtaagga gagattagaa 4320 gacaacaggc atagcaaatg acataagcta ccgattaact aatcggaaca tgtaaaacag 4380 ttacaaaaat aaacgaactc tcctcttgtc ctacaatgaa agccctcatg tgcagtagag 4440 atgcagtttc atcaaagaac aaacatcctt gcaaatgggt gtgacgcggt tccagatgtg 4500 gatuggcaa aacctcattt aagtaaaagg ttagcagagc aaagtgcggt gctttagctg 4560 ctgcttgtgc cgctgtggcg tcggggaggc tcctgcctga gcttccttcc ccagctttgc 4620 tgcctgagag gaaccagagc agacgcacag gccggaaaag gcgcatctaa cgcgtatcta 4680 ggctttggta actgcggaca agttgctttt acctgatttg atgatacatt tcattaaggt 4740 tccagttata aatattttgt taatatttat taagtgacta tagaatgcaa ctccatttac 4800 cagtaactta ttttaaatat gcctagtaac acatatgtag tataatttct agaaacaaac 4860 atctaataag tatataatcc tgtgaaaata tgaggcttga taatattagg ttgtcacgat 4920 gaagcatgct agaagctgta acagaataca tagagaataa tgaggagttt atgatggaac 4980 • 58 ·

95238.doc 1327645 cttaaatata taatgttgcc agcgatttta gttcaatatt tgttactgtt atctatctgc 5040 tgtatatgga attcttttaa ttcaaacgct gaaaagaatc agcatttagt cttgccaggc 5100 acacccaata atcagtcatg tgtaatatgc acaagtttgt ttttgttttt gttttttttg 5160 ttggttggtt tgtttttttg ctttaagttg catgatcttt ctgcaggaaa tagtcactca 5220 tcccactcca cataaggggt ttagtaagag aagtctgtct gtctgatgat ggataggggg 5280 caaatctttt tcccctttct gttaatagtc atcacatttc tatgccaaac aggaacaatr 5340 cataacttta gtcttaatgt acacattgca ttttgataaa attaattttg ttgtttcctt 5400 tgaggttgat cgttgtgttg ttgttttgct gcacttttta cttttttgcg tgtggagctg 5460 tattcccgag accaacgaag cgttgggata cttcattaaa tgtagcgact gtcaacagcg 5520 tgcaggtttt ctgtttctgt gttgtggggt caaccgtaca atggtgtggg agtgacgatg 5580 atgtgaatat ttagaatgta ccatattttt tgtaaattat ttatgttttt ctaaacaaat 5640 ttatcgtata ggttgatgaa acgtcatgtg ttttgccaaa gactgtaaat atttatttat 5700 £ t £ ttcacat ggtcaaaatt tcaccactga aaccctgcac ttagctagaa cctcattttt 5760 aaagattaac aacaggaaat Aaattgtaaa aaaggttttc tatacatgaa aa aaaaaaaa 5820 aaaaaa 5826 < 210 > 95 < 211 > 9645 < 212 > DNA < 213 > human < 400 > 95 atgcccaagc gcgcgcactg gggggccctc tccgtggtgc tgatcctgct ttggggccat 60 ccgcgagtgg cgctggcctg cccgcatcct tgtgcctgct acgtccccag cgaggtccac 120 tgcacgttcc gatccctggc ttccgtgccc gctggcattg ctagacacgt ggaaagaatc 180 aatttggggt ttaatagcat acaggccctg tcagaaacct catttgcagg actgaccaag 240 ttggagctac ttatgattca cggcaatgag atcccaagca tccccgatgg agctttaaga 300 gacctcagct ctcttcaggt tttcaagttc agctacaaca agctgagagt gatcacagga 360 cagaccctcc agggtctctc taacttaatg aggctgcaca ttgaccacaa caagatcgag 420 tttatccacc ctcaagcttt caacggctta acgtctctga ggctactcca tttggaagga 480 aatctcctcc accagctgca ccccagcacc ttctccacgt tcacattttt ggattatttc 540 -59-

95238.doc 1327645 agactctcca ccataaggca cctctactta gcagagaaca tggttagaac tcttcctgcc 600 agcatgcttc £ gaacatgcc gcttctggag aatctttact tgcagggaaa tccgtggacc 660 tgcgattgtg agatgagatg gtttttggaa tgggatgcaa aatccagagg aattctgaag 720 tgtaaaaagg acaaagctta tgaaggcggt cagttgtgtg caatgtgctt cagtccaaag 780 aagttgtaca aacatgagat acacaagctg aaggacatga cttgtctgaa gccttcaata 840 gagtcccctc tgagacagaa caggagcagg agtattgagg aggagcaaga acaggaagag 900 gatggtggca gccagctcat cctggagaaa ttccaactgc cccagtggag catctctttg 960 aatatgaccg acgagcacgg gaacatggtg aacttggtct gtgacatcaa gaaaccaatg 1020 gatgtgtaca agattcactt gaaccaaacg gatcctccag atattgacat aaatgcaaca 1080 gttgccttgg actttgagtg tccaatgacc cgagaaaact atgaaaagct atggaaattg 1140 atagcatact acagtgaagt tcccgtgaag ctacacagag agctcatgct cagcaaagac 1200 cccagagtca gctaccagta caggcaggat gctgatgagg aagctcttta ctacacaggt 1260 gtgagagccc agattcttgc agaaccagaa tgggtcatgc agccatccat agatatccag 1320 ctgaaccgac gtcagagtac ggccaagaag gtgctacttt Cctactacac ccagtattct 1380 caaacaatat ccaccaaaga tacaaggcag gctcggggca gaagctgggt aatgattgag 1440 cctagtggag ctgtgcaaag agatcagact gtcctggaag ggggtccatg ccagttgagc 1500 tgcaacgtga aagcttctga gagtccatct atcttctggg tgcttccaga tggctccatc 1560 ctgaaagcgc ccatggatga cccagacagc aagttctcca ttctcagcag tggctggctg 1620 aggatcaagt ccatggagcc atctgactca ggcttgtacc agtgcattgc tcaagtgagg 1680 gatgaaatgg accgcatggt atatagggta cttgtgcagt ctccctccac tcagccagcc 1740 gagaaagaca cagtgacaat tggcaagaac ccaggggagt cggtgacatt gccttgcaat 1800 gctttagcaa tacccgaagc ccaccttagc tggattcttc caaacagaag gataattaat 1860 gatttggcta acacatcaca tgiatacatg ttgccaaatg gaactctttc catcccaaag 1920 gtccaagtca gtgatagtgg ttactacaga tgtgtggctg tcaaccagca aggggcagac 1980 cattttacgg tgggaatcac agtgaccaag aaagggtctg gcttgccatc caaaagaggc 2040 agacgcccag gtgcaaaggc tctttccaga gtcagagaag acatcgtgga ggatgaaggg 2100 ggctc £ ggca tgggagatga agagaacact tcaaggagac ttctgcatcc aaaggaccaa 2160 -60 ·

95238.doc 1327645 gaggtgttcc tcaaaacaaa ggatgatgcc atcaatggag acaagaaagc caa £ aaaggg 2220 agaagaaagc tgaaactctg gaagcattcg gaaaaagaac cagagaccaa tgttgcagaa 2280 ggtcgcagag tgtttgaatc tagacgaagg ataaacatgg caaacaaaca gattaatccg 2340 gagcgctggg ctgatatttt agccaaagtc cgtgggaaaa atctccctaa gggcacagaa 2400 gtacccccgt tgattaaaac cacaagtcct ccatccttga gcctagaagt cacaccacct 2460 tttcctgctg tttctccccc ctcagcatct cctgtgcaga cagtaaccag tgctgaagaa 2520 tcctcagcag atgtacctct acttggtgaa gaagagcacg ttttgggtac catttcctca 2580 gccagcatgg ggctagaaca caaccacaat ggagttattc ttgttgaacc tgaagtaaca 2640 agcacacctc tggaggaagt ctttctgaga agactgagga gataacttcc 2700 actgaaggag acctgaaggg gacagcagcc cctacactta tatctgagcc ttatgaacca 2760 tctcctactc tgcacacatt agacacagtc tatgaaaagc ccacccatga agagacggca 2820 acagagggtt ggtctgcagc agatgttgga tcgtcaccag agcccacatc cagtgagtat 2880 gagcctccat tggatgctgt ctccttggct gagtctgagc ccatgcaata ctttgaccca 2940 gatttggaga ctaagtcaca accagatgag tgttgatgac Gataagatga aagaagacac ctt tgcacac 3000 cttactccaa cccccaccat ctgggttaat gactccagta catcacagtt atttgaggat 3060 tctactatag gggaaccagg tgtcccaggc caatcacatc tacaaggact gacagacaac 3120 atccaccttg tgaaaagtag tctaagcact caagacacct tactgattaa aaagggtatg 3180 aaagagatgt ctcagacact acagggagga aatatgctag agggagaccc cacacactcc 3240 agaagttctg agagtgaggg ccaagagagc aaatccatca ctttgcctga ctccacactg 3300 ggtataatga gcagtatgtc tccagttaag aagcctgcgg aaaccacagt tggtaccctc 3360 ctagacaaag acaccacaac agtaacaaca acaccaaggc aaaaagttgc tccgtcatcc 3420 accatgagca ctcacccttc tcgaaggaga cccaacggga gaaggagatt acgccccaac 3480 aaattccgcc accggcacaa gcaaacccca cccacaactt ttgccccatc agagactttt 3540 tctactcaac caactcaagc acctgacatt aagatttcaa gtcaagtgga gagttctctg 3600 gttcctacag cttgggtgga taacacagtt aataccccca aacagttgga aatggagaag 3660 aatgcagaac ccacatccaa gggaacacca cggagaaaac acgggaagag gccaaacaaa 3720 catcgatata ccccttctac agigagctca agagcgtccg gatccaagcc cagcccttct 3780 • 61 ·

95238.doc 1327645 ccagaaaata aacatagaaa cattgttact cccagttcag aaactatact tttgcctaga 3840 actgtttctc tgaaaactga gggcccttat gattccttag attacatgac aaccaccaga 3900 aaaatatatt catcttaccc taaagtccaa gagacacttc cagtcacata taaacccaca 3960 tcagatggaa aagaaattaa ggatgatgtt gccacaaatg ttgacaaaca taaaagtgac 4020 attttagtca ctggtgaatc aattactaat gccataccaa cttctcgctc cttggtctcc 4080 actatgggag aatttaagga agaatcctct cctgtaggct ttccaggaac tccaacctgg 4140 aatccctcaa ggacggccca gcctgggagg ctacagacag acatacctgt taccacttct 4200 ggggaaaatc ttacagaccc tccccttctt aaagagcttg aggatgtgga tttcacttcc 4260 gagtttttgt cctctttgac agtctccaca ccatttcacc aggaagaagc tggttcttcc 4320 acaactctct caagcataaa agtggaggtg gcttcaagtc aggcagaaac caccaccctt 4380 gatcaagatc atcttgaaac cactgtggct attctccttt ctgaaactag accacagaat 4440 cacaccccta ctgctgcccg gatgaaggag ccagcatcct cgtccccatc cacaattctc 4500 atgtctttgg gacaa ^ ccac caccactaag ccagcacttc ccagtccaag aatatctcaa 4560 gcatctagag attccaagga aaatgttttc Ttgaattatg tggggaatcc agaa acagaa 4620 gcaaccccag tcaacaatga aggaacacag catatgtcag ggccaaatga attatcaaca 4680 ccctcttccg accgggatgc atttaacttg tctacaaagc tggaattgga aaagcaagta 4740 tttggtagta ggagtctacc acgtggccca gatagccaac gccaggatgg aagagttcat 4800 gcttctcatc aactaaccag agtccctgcc aaacccatcc taccaacagc aacagtgagg 4860 ctacctgaaa tgtccacaca aagcgcttcc agatactttg taacttccca gtcacctcgt 4920 cactggacca acaaaccgga aataactaca tatccttctg gggctttgcc agagaacaaa 4980 cagtttacaa ctccaagatt atcaagtaca acaattcctc tcccattgca catgtccaaa 5040 cccagcattc ctagtaagtt tactgaccga agaactgacc aattcaatgg ttactccaaa 5100 gtgtttggaa ataacaacat ccctgaggca agaaacccag ttggaaagcc tcccagtcca 5160 agaattcctc attattccaa tggaagactc cctttcttta ccaacaagac tctttctttt 5220 ccacagttgg gagtcacccg gagaccccag atacccactt ctcctgcccc agtaatgaga 5280 gagagaaaag ttattccagg ttcctacaac aggatacatt cccatagcac cttccatctg 5340 gactttggcc ctccggcacc tccgttgttg cacactccgc agaccacggg atcaccctca 5400 -62-

95238.doc 1327645 actaacttac agaatatccc tatggtctct tccacccaga gttctatctc ctttataaca 5460 tcttctgtcc agtcctcagg aagcttccac cagagcagct caaagttctt tgcaggagga 5520 cctcctgcat ccaaattctg gtctcttggg gaaaagcccc aaatcctcac caagtcccca 5580 cagactgtgt ccgtcaccgc tgagacagac actgtgttcc cctgtgaggc aacaggaaaa 5640 ccaaagcctt tcgttacttg gacaaaggtt tccacaggag ctcttatgac tccgaatacc 5700 aggatacaac ggtttgaggt tctcaagaac ggtaccttag tgatacggaa ggttcaagta 5760 caagatcgag gccagtatat gtgcaccgcc agcaacctgc acggcctgga caggatggtg 5820 gtcttgcttt cggtcaccgt gcagcaacct caaatcctag cctcccacta ccaggacgtc 5880 actgtctacc tgggagacac cattgcaatg gagtgtctgg ccaaagggac cccagccccc 5940 caaatttcct ggatcttccc tgacaggagg gtgtggcaaa ctgtgtcccc cgtggagagc 6000 cgcatcaccc tgcacgaaaa ccggaccctt tccatcaagg aggcgtcctt ctcagacaga 6060 ggcgtctata agtgcgtggc cagcaatgca gccggggcgg acagcctggc catccgcctg 6120 cacgtggcgg cactgccccc cgttatccac caggagaagc tggagaacat ctcgctgccc 6180 ccggggctca gcattcacat tcactgcact gccaaggctg cgcccctgcc Cagc gtgcgc 6240 t £ ggtgctcg gggacggtac ccagatccgc ccctcgcagt tcctccacgg gaacttgttt 6300 gttttcccca acgggacgct ctacatccgc aacctcgcgc ccaaggacag cgggcgctat 6360 gagtgcgtgg ccgccaacct ggtaggctcc gcgcgcagga cggtgcagct gaacgtgcag 6420 cgtgcagcag ccaacgcgcg catcacgggc acctccccgc ggaggacgga cgtcaggtac 6480 ggaggaaccc tcaagctgga ctgcagcgcc tcgggggacc cctggccgcg catcctctgg 6540 aggctgccgt ccaagaggat gatcgacgcg ctcttcagtt ttgatagcag aatcaaggtg 6600 tttgccaatg ggaccctggt ggtgaaatca gtgacggaca aagatgccgg agattacctg 6660 tgcgtagctc gaaataaggt tggtgatgac tacgtggtgc tcaaagtgga tgtggtgatg 6720 aaaccggcca agattgaaca caaggaggag aacgaccaca aagtcttcta cgggggtgac 6780 ctgaaagtgg actgtgtggc caccgggctt cccaatcccg agatctcctg gagcctccca 6840 gacgggagtc tggtgaactc cttcatgcag tcggatgaca gcggtggacg caccaagcgc 6900 tatgtcgtct tcaacaatgg gacactctac tttaacgaag tggggatgag ggaggaagga 6960 gactacacct gctttgctga aaatcaggtc gggaaggacg agatgagagt cagagtcaag 7020 -63-

95238.doc 1327645 gtggtgacag cgcccgccac catccggaac aagacttact tggcggttca ggtgccctat 7080 ggagacgtgg tcactgiagc ctgtgaggcc aaaggagaac ccatgcccaa ggtgacttgg 7140 ttgtccccaa ccaacaaggt gatccccacc tcctctgaga agtatcagat 7200 ggcactctcc ttattcagaa agcccagcgt tctgacagcg gcaactacac ctgcctggtc 7260 aggaacagcg cgggagagga taggaagacg gtgtggattc acgtcaacgt ccagccaccc 7320 aagatcaacg gtaaccccaa ccccatcacc accgtgcggg agatagcagc cgggggcagt 7380 cggaaactga ttgactgcaa agctgaaggc atccccaccc ataccaagat cgagggtgtt atgggctttt 7440 cccgagggtg tggttctgcc agctccatac tatggaaacc ggatcactgt ccatggcaac 7500 ggttccctgg acatcaggag tttgaggaag agcgactccg tccagctggt atgcatggca 7560 cgcaacgagg gaggggaggc gaggttgatc gtgcagctca ctgtcctgga gcccatggag 7620 aaacccatct tccacgaccc gatcagcgag aagatcacgg ccatggcggg ccacaccatc 7680 agcctcaact gctctgccgc ggggaccccg acacccagcc tggtgtgggt ccttcccaat 7740 ggcaccgatc tgcagagtgg acagcagctg cagcgcttct accacaaggc tgacggcatg 7800 ctacacatta gcggtctctc ctcggtggac gctggggcct accgctgcgt Ggcc cgcaat 7860 gccgctggcc acacggagag gctggtctcc ctgaaggtgg gactgaagcc agaagcaaac 7920 aagcagtatc ataacctggt cagcatcatc aatggtgaga ccctgaagct cccctgcacc 7980 cctcccgggg ctgggcaggg acgtttctcc tggacgctcc ccaatggcat gcatctggag 8040 ggcccccaaa ccctgggacg cgtttctctt ctggacaatg gcaccctcac ggttcgtgag 8100 gcctcggtgt ttgacagggg tacctatgta tgcaggatgg agacggagta cggcccttcg 8160 gtcaccagca tccccgtgat tgtgatcgcc tatcctcccc ggatcaccag cgagcccacc 8220 ccggtcatct acacccggcc cgggaacacc gtgaaactga actgcatggc tatggggatt 8280 cccaaagctg acatcacgtg ggagttaccg gataagtcgc atctgaaggc aggggttcag 8340 gctcgtctgt atggaaacag atttcttcac ccccagggat cactgaccat ccagcatgcc 8400 acacagagag atgccggctt ctacaagtgc atggcaaaaa acattctcgg cagtgactcc 8460 aaaacaactt acatccacgt cttctgaaat gtggattcca gaatgattgc ttaggaactg 8520 acaacaaagc ggggtttgta agggaagcca ggttggggaa taggagctct taaataatgt 8580 gtcacagtgc atggtggcct ctggtgggtt tcaagttgag gttgatcttg atctacaatt 8640 -64 ·

95238.doc 1327645 gttgggaaaa ggaagcaatg cagacacgag aaggagggct cagccttgct gagacacttt 8700 cttttgtgtt tacatcatgc caggggcttc attcagggtg tctgtgctct gactgcaatt 8760 tttcttcttt tgcaaatgcc actcgactgc cttcataagc gtccatagga tatctgagga 8820 acattcatca aaaataagcc atagacatga acaacacctc actaccccat tgaagacgca 8880 tcacctagtt aacctgctgc agtttttaca tgatagactt tgttccagat tgacaagtca 8940 tctttcagtt atttcctctg tcacttcaaa actccagctt gcccaataag gatttagaac 9000 cagagtgact gatatatata tatatatttt aattcagagt tacatacata cagctaccat 9060 tttatatgaa aaaagaaaaa catttcttcc tggaactcac tttttatata atgttttata 9120 tatatatttt ttcctttcaa atcagacgat gagactagaa ggagaaatac tttctgtctt 9180 attaaaatta ataaattatt ggtctttaca agacttggat acattacagc agacatggaa 9240 atataatttt aaaaaatttc tctccaacct ccttcaaatt cagtcaccac tgttatatta 9300 ccttctccag gaaccctcca gtggggaagg ctgcgatatt agatttcctt gtatgcaaag 9360 tttttgttga aagctgtgct cagaggaggt gagaggagag gaaggagaaa actgcatcat 9420 aactttacag aattgaatct agagtcttcc ccgaaaagcc cagaaacttc Tctg cagtat 9480 ctggcttgtc catctggtct aaggtggctg cttcttcccc agccatgagt cagtttgtgc 9540 ccatgaataa tacacgacct gttatttcca tgactgcttt actgtatttt taaggtcaat 9600 atactgtaca tttgataata aaataatatt ctcccaaaaa aaaaa 9645 < 210> 96 < 211 > 694 < 212 > DNA < 213 > human < 400 > 96 gcciccgagg agaccatggc ctggcccctg Tgcaccctgc tgctcctgct ggccacccag 60 gctgtggccc tggcctggag cccccaggag gaggacagga taatcgaggg tggcatctat 120

V gatgcagacc tcaatgatga gcgggtacag cgtgcccttc actttgtcat cagcgagtat 180 aacaaggcca ctgaagatga gtactacaga cgcctgctgc gggtgctacg agccagggag 240 cagatcgtgg gcggggtgaa ttacttcttc gacatagagg tgggccgaac catatgtacc 300 aagtcccagc ccaacttgga cacctgtgcc ttccatgaac agccagaact gcagaagaaa 360 -65-

95238.doc 1327645 cagttgtgct ctttccagat ctacgaagtt ccctgggagg acagaatgtc cctggtgaat 420 tccaggtgtc aagaagccta gggatctgtg ccagggagtc acactgacca cctcctactc 480 ccaccccttg tagtgctccc acccctggac tggtggcccc caccctgtgg gaggtctccc 540 catgcacctg cagcaggaga agacagagaa ggctgcagga ggcctttgtt gctcagcagg 600 ggactctgcc ctccctcctt ccttttgctt ctcatagccc tggtacatgg tacacacacc 660 cccacctcct gcaattaaac agtagcatca cctc 694 < 210 > 97 < 211 > 782 < 212 > DNA < 213 > human < 400 > 97 gggctccctg cctcgggctc tcaccctcct ctcctgcagc tccagctttg tgctctgcct 60 ctgaggagac catggcccag tatctgagta ccctgctgct cctgctggcc accctagctg 120 tggccctggc ctggagcccc aaggaggagg ataggataat cccgggtggc atctataacg 180 cagacctcaa tgatgagtgg gtacagcgtg cccttcactt cgccatcagc gagtataaca 240 aggccaccaa agatgactac tacagacgtc Cgctgcgggt actaagagcc aggcaacaga 300 ccgttggggg ggtgaattac ttcttcgacg tagaggtggg ccgcaccata tgtaccaagt 360 cccagcccaa cttggacacc tgtgccttcc atgaacagcc agaactgcag aagaaacagt 420 tgtgctcttt cgagatctac gaagttccct gggagaacag aaggtccctg gtgaaatcca 480 ggtgtcaaga atcctaggga tctgtgccag gccattcgca ccagccacca cccactccca 540 ccccctgtag tgctcccacc cctggactgg tggcccccac cctgcgggag gcctccccat 600 gtgcctgcgc caagagacag acagagaagg ctgcaggagt cctttgttgc tcagcagggc 660 gctctgccct ccctccttcc ttcttgcttc taatagccct ggtacatggt acacaccccc 720 ccacctcctg caattaaaca gtagcatcgc ctccctctga aaaaaaaaaa aaaaaaaaaa 780 aa 782 < 210 > 98 < 211 > 3432 < 212 > DNA < 213> human < 400 > 98 -66 · 95238.doc 1327645 actccagcgc gcggctacct acgcttggtg cttgctttct ccagccatcg gagaccagag 60 cc £ ccccctc tgctcgagaa aggggctcag cggcggcgga agcggagggg gaccaccgtg 120 gagagcgcgg tcccagcccg gccactgcgg atccctgaaa ccaaaaagct cctgctgctt 180 ctgtaccccg cctgtccctc ccagctgcgc Agggcccctt cgtgggatca tcagcccgaa 240 gacagggatg gagaggcctc tgtgctccca cctctgcagc tgcctggcta tgctggccct 300 cctgtccccc ctg3gcctgg cacagtatga c3gctggccc c3ttaccccg 3gtacttcca 3δ0 gcaaccggct cctgagtatc accagcccca ggcccccgcc aacgtggcca agattcagct 420 gcgcctggct gggcagaaga ggaagcacag cgagggccgg gtggaggtgt actatgatgg 480 ccagtggggc accgtgtgcg atgacgactt ctccatccac gctgcccacg tcgtctgccg 540 ggagctgggc tatgtggagg ccaagtcctg gactgccagc tcctcctacg gcaagggaga 600 agggcccatc tggttagaca atctccactg tactggcaac gaggcgaccc ttgcagcatg 660 cacctccaat ggctggggcg tcactgactg caagcacacg gaggatgtcg gtgtggtgtg 720 cagcgacaaa aggattcctg ggttcaaatt tgacaattcg ttgatcaacc agatagagaa 780 cctgaatatc caggtggagg acattcggat tcgagccatc ctctcaacct accgcaagcg 840 caccccagtg atggagggct acgtggaggt gaaggagggc aagacctgga agcagatctg 900 tgacaagcac tggacggcca agaattcccg cgtggtctgc ggcatgtttg gcttccctgg 960 ggagaggaca tacaatacca aagtgtacaa aatgtttgcc tcacggagga agcagcgcta 1020 ctggccattc tccatggact gcaccggcac agaggcccac atctccagct gcaagctggg 1080 cccccaggtg tcactggacc ccatgaagaa tgtcacctgc gagaatgggc tgccggccgt 1140 ggtgagttgt gtgcctgggc a four tcttcag ccctgacgga ccctcgagat tccggaaagc 1200 atacaagcca gagcaacccc tggtgcgact £ agaggcggt gcctacatcg gggag £gccg 1260 cgtggaggtg ctcaaaaatg gagaatgggg gaccgtctgc gacgacaagt gggacctggt 1320 gtcggccagt gtggtctgca gagagctggg ctttgggagt gccaaagagg cagtcactgg 1380 ctcccgactg gggcaaggga tcggacccat ccacctcaac gagatccagt gcacaggcaa 1440 tgagaagtcc attatagact gcaagttcaa tgccgagtct cagggctgca accacgagga 1500 ggatgctggt gtgagatgca acacccctgc catgggcttg cagaagaagc tgcgcctgaa 1560 cggcggccgc aatccctacg agggccgagt ggaggtgctg gtggagagaa acgggtccct 1620 -67-

95238.doc 1327645 tgtgtggggg atggtgtgtg gccaaaactg gggcatcgtg gaggccatgg tggtctgccg 1680 ccagctgggc ctgggattcg ccagcaacgc cttccaggag acctggtatt ggcacggaga 1740 tgtcaacagc aacaaagtgg tcatgagtgg agtgaagtgc tcgggaacgg agctgtccct 1800 ggcgcactgc cgccacgacg gggaggacgt ggcctgcccc cagggcggag tgcagtacgg 1860 ggccggagtt gcctgctcag aaaccgcccc tgacctggtc ctcaatgcgg agatggtgca 1920 gcagaccacc tacctggagg accggcccat gttcatgctg cagtgtgcca tggaggagaa 1980 ctgcctctcg gcctcagccg cgcagaccga ccccaccacg ggctaccgcc ggctcctgcg 2040 cttctcctcc cagatccaca acaatggcca gtccgacttc cggcccaaga acggccgcca 2100 cgcgtggatc tggcacgact gtcacaggca ctaccacagc atggaggtgt tcacccacta 2160 tgacctgctg aacctcaatg gcaccaaggt ggcagagggc cacaaggcca gcttctgctt 2220 ggaggacaca gaatgtgaag gagacatcca gaagaattac gagtgtgcca acttcggcga 2280 tcagggcatc accatgggct gctgggacat gtaccgccat gacatcgact gccagtgggt 2340 tgacatcact gacgtgcccc ctggagacta cctgttccag gttgttatta accccaactt 2400 cgaggttgca gaatccgatt actccaacaa catcatgaaa tgcaggagcc Gcta tgacgg 2460 ccaccgcatc tggatgtaca actgccacat aggtggttcc ttcagcgaag agacggaaaa 2520 aaagtttgag cacttcagcg ggctcttaaa caaccagctg tccccgcagt aaagaagcct 2580 gcgtggtcaa ctcctgtctt caggccacac cacatcttcc atgggacttc cccccaacaa 2640 ctgagtctga acgaatgcca cgtgccctca cccagcccgg cccccaccct gtccagaccc 2700 ctacagctgt gtctaagctc aggaggaaag ggaccctccc atcattcatg gggggctgct 2760 acctgaccct tggggcctga gaaggccttg ggggggtggg gtttgtccac agagctgctg 2820 gagcagcacc aagagccagt cttgaccggg atgaggccca cagacaggtt gtcatcagct 2880 tgtcccattc aagccaccga gctcaccaca gacacagtgg agccgcgctc ttctccagtg 2940 acacgtggac aaatgcgggc tcatcagccc ccccagagag ggtcaggccg aaccccattt 3000 ctcctcctct taggtcattt tcagcaaact tgaatatcta gacctctctt ccaatgaaac 3060 cctccagtct attatagtca catagataat ggtgccacgt gttttctgat ttggtgagct 3120 cagacttggt gcttccctct ccacaacccc caccccttgt ttttcaagat actattatta 3180 tattttcaca gacttttgaa gcacaaattt attggcattt aatattggac atctgggccc 3240 -68-

95238.doc 1327645 ttggaagtac aaatctaagg aaaaaccaac ccactgtgla agtgactcat ctlcctgttg 3300 ttccaattct gtgggttttt gattcaacgg tgctataacc agggtrctgg gtgacagggc 3360 gctcactgag caccatgtgt catcacagac acttacacat acttgaaact tggaataaaa 3420 gaaagattta tg 3432 < 210 > 99 < 211 > 8448 < 212 > DNA < 2i3 > human < 400 > 99 gcagtggttt ctcctccttc ctcccaggaa gggccaggaa aatggccctg gtcctggaga 60 tcttcaccct gctggcctcc atctgctggg tgtcggccaa tatcttcgag taccaggttg 120 atgcccagcc ccttcgtccc tgtgagctgc agagggaaac ggcctttctg aagcaagcag 180 actacgtgcc ccagtgtgca gaggatggca gcttccagac tgtccagtgc cagaacgacg 240 gccgctcctg ctggtgtgtg ggtgccaacg gcagtgaagt gctgggcagc aggcagccag 300 gacggcctgt ggcttgtctg tcattttgtc agctacagaa acagcagatc ttactgagtg 360 gctacattaa cagcacagac acctcctacc tccctcagtg tcaggattca Ggggactacg 420 cgcctgttca gtgtgatgtg cagcatgtcc agtgctggtg tgtggacgca gaggggatgg 480 aggtgtatgg gacccgccag ctggggaggc caaagcgatg tccaaggagc tgtgaaataa 540 gaaatcgtcg tcttctccac ggggtgggag ataagtc acc accccagtgt tctgcggagg 600 gagagtttat gcctgtccag tgcaaatttg tcaacaccac agacatgatg atttttgatc 660 tggtccacag ctacaacagg tttccagatg catttgtgac cttcagttcc ttccagagga 720 ggttccctga ggtatctggg tattgccact gtgctgacag ccaagggcgg gaactggctg 780 agacaggttt ggagttgtta ctggatgaaa tttatgacac catttttgct ggcctggacc 840 ttccttccac cttcactgaa accaccctgt accggatact gcagagacgg ttcctcgcag 900 ttcaatcagt catctctggc agattccgat gccccacaaa atgtgaagtg gagcggttta 960 cagcaaccag ctttggtcac ccctatgttc caagctgccg Ccgaaatggc gactatcagg 1020 cggtgcagtg ccagacggaa gggccctgct ggtgtgtgga cgcccagggg aaggaaatgc 1080 atggaacccg gcagcaaggg gagccgccat cttgtgctga aggccaatct tgtgcctccg 1140 aaaggcagca ggccttgtcc agactctact ttgggacctc aggctacttc agccagcacg 1200 •69·

95238.doc 1327645 acctgttctc ttccccagag aaaagatggg cctctccaag agtagccaga tttgccacat 1260 cctgcccacc cacgatcaag gagctctttg tggactctgg gcttctccgc ccaatggtgg 1320 agggacagag ccaacagttt tctgtctcag aaaatcttct caaagaagcc atccgagcaa 1380 tttttccctc ccgagggctg gctcgtcttg cccttcagtt taccaccaac ccaaagagac 1440 tccagcaaaa cctttttgga gggaaatttt tggtgaatgt tggccagttt aacttgtctg 1500 gagcccttgg cacaagaggc acatttaact tcagtcaatt tttccagcaa cttggtcttg 1560 caagcttctt gaatggaggg agacaagaag atttggccaa gccactctct gtgggattag 1620 attcaaattc ttccacagga acccctgaag ctgctaagaa ggatggtact atgaataagc 1680 caactgtggg cagctttggc tttgaaatta acctacaaga gaaccaaaat gccctcaaat 1740 tccttgcttc tctcctggag cttccagaat tccttctctt cttgcaacat gctatctctg 1800 tgccagaaga tgtggcaaga gatttaggtg atgtgatgga aacggtactc gactcccaga 1860 cctgtgagca gacacctgaa aggctatttg tcccatcatg cacgacagaa ggaagctatg 1920 aggatgtcca atgcttttcc ggagagtgct ggtgtgtgaa ttcctggggc aaagagcttc 1980 caggctcaag agtcagagat ggacagccaa ggtgccccac agactgtgaa Aagc aaaggg 2040 ctcgcatgca aagcctcatg ggcagccagc ctgctggctc caccttgttt gtccctgctt 2100 gtactagtga gggacatttc ctgcctgtcc agtgcttcaa ctcagagtgc tactgtgttg 2160 atgctgaggg tcaggccatt cctggaactc gaagtgcaat agggaagccc aagaaatgcc 2220 ccacgccctg tcaattacag tctgagcaag ctttcctcag gacggtgcag gccctgctct 2280 ctaactccag catgctaccc accctttccg acacctacat cccacagtgc agcaccgatg 2340 ggcagtggag acaagtgcaa tgcaatgggc ctcctgagca ggtcttcgag ttgtaccaac 2400 gatgggaggc tcagaacaag ggccaggatc tgacgcctgc caagctgcta gtgaagatca 2460 tgagctacag agaagcagct tccggaaact tcagtctctt tattcaaagt ctgtatgagg 2520 ctggccagca agatgtcttc ccggtgctgt cacaataccc ttctctgcaa gatgtcccac 2580 tagcagcact ggaagggaaa cggccccagc ccagggagaa tatcctcctg gagccctacc 2640 tcttctggca gatcttaaat ggccaactca gccaataccc ggggtcctac tcagacttca 2700 gcactccttt ggcacatttt gatcttcgga actgctggtg tgtggatgag gctggccaag 2760 aactggaagg aatgcggtct gagccaagca agctcccaac gtgtcctggc tcctgtgagg 2820 -70 · 95238.doc 1327645 Aagcaaagct ccgtgtactg cagttcatta gggaaacg ga agagattgtt tcagcttcca 2880 acagttctcg gttccctctg ggggagagtt tcctggtggc caagggaatc cggctgagga 2940 atgaggacct cggccttcct ccgctcttcc cgccccggga ggctttcgcg gagtttctgc 3000 gtgggagtga ttacgccatt cgcctggcgg ctcagtctac cttaagcttc tatcagagac 3060 gccgcttttc cccggacgac tcggctggag catccgccct tctgcggtcg ggcccctaca 3120 tgccacagtg tgatgcgttt ggaagttggg agcctgtgca gtgccacgct gggactgggc 3180 actgctggtg tgtagatgag aaaggagggt tcatccctgg ctcactgact gcccgctctc 3240 tgcagattcc acagtgcccg acaacctgcg agaaatctcg aaccagtggg ctgctttcca 3300 gttggaaaca ggctagatcc caagaaaacc catctccaaa agacctgttc gtcccagcct 3360 gcctagaaac aggagaatat gccaggctgc aggcatcggg ggctggcacc tggtgtgtgg 3420 accctgcatc aggagaagag ttgcggcctg gctcgagcag cagtgcccag tgcccaagcc 3480 tctgcaatgt gctcaagagt ggagtcctct ctaggagagt cagcccaggc tatgtcccag 3540 cctgcagggc agaggatggg ggcttttccc cagtgcaatg tgaccaggcc cagggcagct 3600 gctggtgtgt catggacagc ggagaagagg tgcctgggac gcgcgtgacc gggggccagc 3660 ccgcctgtga gagcccgcgg tgtccgctgc cattcaacgc gtc ggaggtg gttggtggaa 3720 caatcctgtg tgagacaatc tcgggcccca caggctctgc catgcagcag tgccaattgc 3780 tgtgccgcca aggctcctgg agcgtgtttc caccagggcc attgatatgt agcctggaga 3840 gcggacgctg ggagtcacag ctgcctcagc cccgggcctg ccaacggccc cagctgtggc 3900 agaccatcca gacccaaggg cactttcagc tccagctccc gccgggcaag atgtgcagtg 3960 ctgactacgc gggttt £ dg cagactttcc aggttttcat attggatgag ctgacagccc 4020 gcggcttctg ccagatccag gtgaagactt ttggcaccct ggtttccatt cctgtctgca 4080 acaactcctc tgtgcaggtg ggttgtctga ccagggagcg tttaggagtg aatgttacat 4140 ggaaatcacg gcttgaggac atcccagtgg cttctcttcc tgacttacat gacattgaga 4200 gagccttggt gggcaaggat ctccttgggc gcttcacaga tctgatccag agtggctcat 4260 tccagcttca tctggactcc aagacgttcc cagcggaaac catccgcttc ctccaagggg 4320 accactttgg cacctctcct aggacacggt ttgggtgctc ggaaggattc taccaagtct 4380 tgacaagtga ggccagtcag gacggactgg gatgcgttaa gtgccatgaa ggaagctatt 4440 -71 -

95238.doc 1327645 cccaagatga ggaatgcatt ccttgtcctg ttggattcta ccaagaacag gcagggagct 4500 tggcctgtgt cccatgtcct gtgggcagaa cgaccatttc tgccggagct ttcagccaga 4560 ctcactgtgt cactgactgt cagaggaacg aagcaggcct gcaat £ tgac cagaatggcc 4620 agtatcgagc cagccagaag gacaggggca gtgggaaggc cttctgtgtg gacggcgagg 4680 ggcggaggct gccatggtgg gaaacagagg cccctcttga ggactcacag tgtttgatga 4740 tgcagaagtt tgagaaggtt ccagaatcaa aggtgatctt cgacgccaat gctcctgtgg 4800 ctgtcagatc caaagttcct gattctgagt tccccgtgat gcagtgcttg acagattgca 4860 cagaggacga ggcctgcagc ttcttcaccg tgtccacgac ggagccagag atttcctgtg 4920 atttctatgc ttggacaagt gacaatgttg cctgcatgac ttctgaccag aaacgagatg 4980 cactggggaa ctcaaaggcc accagctttg gaagtcttcg ctgccaggig aaagtgagga 5040 gccatggtca agattctcca gctgtgtatt tgaaaaaggg ccaaggatcc accacaacac 5100 ttcagaaacg ctttgaaccc actggtttcc aaaacatgct ttctggattg tacaacccca 5160 ttgtgttctc agcctcagga gccaatctaa ccgatgctca cctcttctgt cttcttgcat 5220 gcgaccgtga tctgtgttgc gatggcttcg Tcctcacaca ggttcaagga ggt gccatca 5280 tctgtgggtt gctgagctca cccagtgtcc tgctttgtaa tgtcaaagac tggatggatc 5340 cctctgaagc ctgggctaat gctacatgtc ctggtgtgac atatgaccag gagagccacc 5400 aggtgatatt gcgtcttgga gaccaggagt tcatcaagag tctgacaccc ttagaaggaa 5460 ctcaagacac ctttaccaat tttcagcagg tttatctctg gaaagattct gacatggggt 5520 ctcggcctga gtctatggga tgtagaaaaa acacagtgcc aaggccagca tctccaacag 5580 aagcaggttt gacaacagaa cttttctccc ctgtggacct caaccaggtc attgtcaatg 5640 gaaatcaatc actatccagc cagaagcact ggcttttcaa gcacctgttt tcagcccagc 5700 aggcaaacct atggtgcctt tctcgttgtg tgcaggagca ctctttctgt cagctcgcag 5760 agataacaga gagtgcatcc ttgtacttca cctgcaccct ctacccagag gcacaggtgt 5820 gtgatgacat catggagtcc aatacccag £ gctgcagact gatcctgcct cagatgccaa 5880 aggccctgtt ccggaagaaa gttatactgg aagataaagt gaagaacttt tacactcgcc 5940 tgccgttcca aaaactgatg gggatatcca ttagaaataa agtgcccatg tctgaaaaat 6000 ctatttctaa tgggttcttt gaatgtgaac gacggtgcga tgcggaccca tgctgcactg 6060 -72-

95238.doc 1327645 gctttggatt tctaaatgtt tcccagttaa aaggaggaga ggtgacatgt ctcactctga 6120 acagcttggg aattcagatg tgcagtgagg agaatggagg agcctggcgc attttggact 6180 gtggctctcc tgacattgaa gtccacacct atcccttcgg atggtaccag aagcccattg 6240 ctcaaaataa tgctcccagt ttttgccctt tggttgttct gccttccctc acagagaaag 6300 tgtctctgga atcgtggcag tccctggccc tctcttcagt ggttgttgat ccatccatta 6360 ggcactttga tgttgcccat gtcagcactg ctgccaccag caatttctct gctgtccgag 6420 acctctgttt gtcggaatgt tcccaacatg aggcctgtct catcaccact ctgcaaaccc 6480 aactc stepped ggc tgtgagatgt atgttctatg ctgatactca aagctgcaca catagtctgc 6540 agggtcggaa ctgccgactt ctgcttcgtg aagaggccac ccacatctac cggaagccag 6600 gaatctctct gctcagctat gaggcatctg taccttctgt gcccatttcc acccatggcc 6660 ggctgctggg caggtcccag gccatccagg tgggtacctc atggaagcaa gtggaccagt 6720 tccttggagt tccatatgct gccccgcccc tggcagagag gcacttccag gcaccagagc 6780 ccttgaactg gacaggctcc tgggatgcca gcaagccaag ggccagctgc tggcagccag 6840 gcaccagaac atccacgtct cctggagtca Gtgaagattg tttgtatctc aat gtgttca 6900 tccctcagaa tgtggcccct aacgcgtctg tgctggtgtt cttccacaac accatggaca 6960 gggaggagag tgaaggatgg ccggctatcg acggctcctt cttggctgct gttggcaacc 7020 tcatcgtggt cactgccagc taccgagtgg gtgtcttcgg cttcctgagt tctggatccg 7080 gagaggtgag tggcaactgg gggctgctgg accaggtggc ggctctgacc tgggtgcaga 7140 cccacatccg aggatttggc ggggaccctc ggcgcgtgtc cctggcagca gaccgtggcg 7200 gggctgatgt ggccagcatc caccttctca cggccagggc caccaactcc caacttttcc 7260 ggagagctgt gctgatggga ggctccgcac tctccccggc cgccgtcatc agccatgaga 7320 gggctcagca gcaggcaatt gctttggcaa aggaggtcag ttgccccatg tcatccagcc 7380 aagaagtggt gtcctgcctc cgccagaagc ctgccaatgt cctcaatgat gcccagacca 7440 agctcctggc cgtgagtggc cctttccact actggggtcc tgtgatcgat ggccacttcc 7500 tccgtgagcc tccagccaga gcactgaaga ggtctttatg ggtagaggtc gatctgctca 7560 ttgggagttc tcaggacgac gggctcatca acagagcaaa ggctgtgaag caatttgagg 7620 aaagtcgagg ccggaccagt agcaaaacag ccttttacca ggcactgcag aattctctgg 7680 -73-

95238.doc 1327645 gtggcgagga ctcagatgcc cgcgtcgagg ctgctgctac atggtattac tctctggagc 7740 actccacgga tgactatgcc tccttctccc gggctctgga gaatgccacc cgggactact 7800 ttatcatctg ccctataatc gacatggcca gtgcctgggc aaagagggcc cgaggaaacg 7860 tcttcatgta ccatgctcct gaaaactacg gccatggcag cctggagctg ctggcggatg 7920 ttcagtttgc cttggggctt cccttctacc cagcctacga ggggcagttt tctctggagg 7980 agaagagcct gtcgctgaaa atcatgcagt acttttccca cttcatcaga tcaggaaatc 8040 ccaactaccc ttatgagttc tcacggaaag tacccacatt tgcaaccccc tggcctgact 8100 ttgtaccccg tgctggtgga gagaactaca aggagttcag tgagctgctc cccaatcgac 8160 agggcctgaa gaaagccgac tgctccttct ggtccaagta catctcgtct ctgaagacat 8220 ctgcagatgg agccaagggc gggcagtcag cagagagtga agaggaggag ttgacggctg 8280 gatctgggct aagagaagat ctcctaagcc tccaggaacc aggctctaag acctacagca 8340 agtgaccagc ccttgagctc cccaaaaacc tcacccgagg ctgcccacta tggtcatctt 8400 tttctctaaa atagttactt accttcaata aagtatctac atgcggtg 8448 < 210 > 100 <211> 5025 <212> DNA <213> Human <4 00 > 100 gcagtggttt ctcctccttc ctcccaggaa gggccaggaa aatggccctg gtcctggaga 60 tcttcaccct gctggcctcc atctgctggg tgtcggccaa tatcttcgag taccaggttg 120 atgcccagcc ccttcgtccc tgtgagctgc agagggaaac ggcctttctg aagcaagcag 180 actacgtgcc ccagtgtgca gaggatggca gcttccagac tgtccagtgc cagaacgacg 240 gccgctcctg ctggtgtgtg ggtgccaacg gcagtgaagt gctgggcagc aggcagccag 300 gacggcctgt ggcttgtctg tcattttgtc agctacagaa acagcagatc ttactgagtg 360 gctacattaa cagcacagac acctcctacc tccctcagtg tcaggattca Ggggactacg 420 cgcctgttca gtgtgatgtg cagcatgtcc agtgctggtg tgtggacgca gaggggatgg 480 aggtgtatgg gacccgccag ctggggaggc caaagcgatg tccaaggagc tgtgaaataa 540 gaaatcgtcg tcttctccac ggggtgggag ataagtcacc accccagtgt tctgcggagg 600 -74-

95238.doc 1327645 gagagtttat gcctgtccag tgcaaatttg tcaacaccac agacatgatg atttttgatc 660 tggtccacag ctacaacagg tttccagatg catttgtgac cttcagttcc ttccagagga 720 ggttccctga ggtatctggg tattgccact gtgctgacag ccaagggcgg gaactggctg 780 agacaggttt ggagttgtta ctggatgaaa tttatgacac catttttgct ggcctggacc 840 ttccttccac cttcactgaa accaccctgt accggatact gcagagacgg ttcctcgcag 900 ttcaatcagt catctctggc agattccgat gccccacaaa atgtgaagtg gagcggttta 960 cagcaaccag ctttggtcac ccctatgttc caagctgccg ccgaaatggc gactatcagg 1020 cggtgcagtg ccagacggaa gggccctgct ggtgtgtgga cgcccagggg aaggaaatgc 1080 atggaacccg gcagcaaggg gagccgccat cttgtgctga aggccaatct tgtgcctccg 1140 aaaggcagca ggccttgtcc agactctact ttgggacctc aggctacttc agccagcacg 1200 acctgttctc ttccccagag aaaagatggg cctctccaag agtagccaga tttgccacat 1260 cctgcccacc cacgatcaag gagctctttg tggactctgg gcttctccgc ccaatggtgg 1320 agggacagag ccaacagttt tctgtctcag aaaatcttct caaagaagcc atccgagcaa 1380 tttttccctc ccgagggctg gctcgtcttg cccttcagtt taccaccaac Ccaaagagac 1440 tccagcaaaa cctttttgga gggaaatttt tggtgaatgt tggccagttt aacttgtctg 1500 gagcccttgg cacaagaggc acatttaact tcagtcaatt tttccagcaa cttggtcttg 1560 caagcttctt gaatggaggg agacaagaag atttggccaa gccactctct gtgggattag 1620 attcaaattc ttccacagga acccetgaag ctgctaagaa ggatggtact atgaataagc 1680 caactgtggg cagctttggc tttgaaatta acctacaaga gaaccaaaat gccctcaaat 1740 tccttgcttc tctcctggag cttccagaat tccttctctt cttgcaacat gctatctctg 1800 tgccagaaga tgtggcaaga gatttaggtg atgtgatgga aacggtactc gactcccaga 1860 cctgtgagca gacacctgaa aggctatttg tcccatcatg cacgacagaa ggaagctatg 1920 aggatgtcca atgcttttcc ggagagtgct ggtgtgtgaa ttcctggggc aaagagcttc 1980 caggctcaag agtcagagat ggacagccaa ggtgccccac agactgtgaa aagcaaaggg 2040 ctcgcatgca aagcctcatg ggcagccagc ctgctggctc caccttgttt gtccctgctt 2100 gtactagtga gggacatttc ctgcctgtcc agtgcttcaa ctcagagtgc tactgtgttg 2160 atgctgaggg tcaggccatt cctggaactc gaagtgcaat agggaagccc aagaaatgcc 2220 -75-

95238.doc 1327645 ccacgccctg tcaattacag tctgagcaag ctttcctcag gacggtgcag gccctgctct 2280 acctccctcc gcggagcagc cagacagcga gggccccggc cgggggcagg ggggacgccc 2340 cgtccggggc accccccccg gctctgagcc gcccgcgggg ccggcctcgg cccggagcgg 2400 aggaaggagt cgcc £ agga £ cagcctgagg ccccagagtc tgagacgagc cgccgccgcc 2460 ccxgccactg c which gaggag g Qing aggagg agcgggagga gggacgagct ggtcgggag ^ agag £ aaaaa aacttttgag acttttccgt tgccgctggg agccggaggc gcggggacct 2580 cttggcgcga cgctgccccg cgaggaggca ggacttgggg accccagacc gcctcccra 2640 gccgccgggg acgcttgctc cctccctgcc ccctacacgg cgtccctcag gcgcccccat 2700 tccggaccag ccctcgggag tcgccgaccc ggcctcccgc aaagactttt ccccagacct 2760 cgggcgcacc ccctgcacgc cgccttcatc cccggcctgt ctcctgagcc cccgcgcatc 2820 ctagaccctt tctcctccag gagacggatc tctctccgac ctgccacaga tcccctattc 2880 aagaccaccc accttctggt accagatcgc gcccatctag gttatttccg tgggatactg 2940 agacaccccc ggtccaagcc tcccctccac Cactgcgccc ttctccctga ggagcctcag 3000 ctttccctcg aggccctcct accttttgcc gggagacccc cagcccctgc agg ggcgggg 3060 cctccccacc acaccagccc tgttcgcgct ctcggcagtg ccggggggcg ccgcctcccc 3120 catgccgccc tccgggctgc ggctgctgcc gctgctgcta ccgctgctgt ggctactggt '3180 gctgacgcct ggcccgccgg ccgcgggact atccacctgc aagactatcg acatggagct 3240 ggtgaagcgg aagcgcatcg aggccatccg cggccagatc ctgtccaagc tgcggctcgc 3300 cagccccccg agccaggggg aggtgccgcc cggcccgctg cccgaggccg tgctcgccct 3360 gtacaacagc acccgcgacc gggtggccgg ggagagtgca gaaccggagc ccgagcctga 3420 ggccgactac tacgccaagg aggtcacccg cgtgctaatg gtggaaaccc acaacgaaat 3480 ctatgacaag ttcaagcaga gtacacacag catatatatg ttcttcaaca catcagagct 3540 ccgagaagcg gtacctgaac ccgtgttgct ctcccgggca gagctgcgtc tgctgaggag 3600 gctcaagtta aaagtggagc agcacgtgga gctgtaccag aaatacagca acaattcctg 3660 gcgatacctc agcaaccggc tgctggcacc cagcgactcg ccagagtggt tatcttttga 3720 tgtcaccgga gttgtgqggc agtggttgag ccgtggaggg gaaattgagg gctttcgcct 3780 tagcgcccac tgctcctgtg acagcaggga taacacactg caagtggaca tcaacgggtt 3840 -76 ·

95238.doc 1327645 cactaccggc cgccgaggtg acctggccac cattcatggc atgaaccggc ctttcctgct 3900 tctcatggcc accccgctgg agagggccca gcatctgcaa agctcccggc accgccgagc 3960 cctggacacc aactattgct tcagctccac ggagaagaac tgctgcgtgc ggcagctgta 4020 cattgacttc cgcaaggacc tcggctggaa gtggatccac gagcccaagg gctaccatgc 4080 caacttctgc ctcgggccct gcccctacat ttggagcctg gacacgcagt acagcaaggt 4140 cctggccctg tacaaccagc ataacccggg cgcctcggcg gcgccgtgct gcgtgccgca 4200 ggcgctggag ccgctgccca tcgtgtacta cgtgggccgc aagcccaagg tggagcagct 4260 gtccaacatg atcgtgcgct cctgcaagtg cagctgaggt cccgccccgc cccgccccgc 4320 cccggcaggc ccggccccac cccgccccgc ccccgctgcc ttgcccatgg gggctgtatt 4380 taaggacacc gtgccccaag cccacctggg gccccattaa agatggagag aggactgcgg 4440 atctctgtgt cattgggcgc ctgcctgggg tctccatccc tgacgttccc ccactcccac 4500 tccctctctc tccctctctg cctcctcctg cctgtctgca ctattccttt gcccggcatc 4560 aaggcacagg ggaccagtgg ggaacactac tgtagttaga tctatttatt gagcaccttg 4620 ggcactgttg aagtgcctta cattaatgaa ctcattcagt caccatagca Acac tctgag 4680 atggcaggga ctctgataac acccatttta aaggttgagg aaacaagccc agagaggtta 4740 agggaggagt tcctgcccac caggaacctg ctttagtggg ggatagtgaa gaagacaata 4800 aaagatagta gttcaggcca ggcggggtgc tcacgcctgt aatcctagca cttttgggag 4860 gcagagatgg gaggatactt gaatccaggc atttgagacc agcctgggta acatagtgag 4920 accctatctc tacaaaacac ttttaaaaaa tgtacacctg tggtcccagc tactctggag 4980 gctaaggtgg gaggatcact tgatcctggg aggtcaaggc tgcag 5025 < 210 > 101 < 211 > 2208 < 212 > DNA < 213 > human < 400 > 101 tctttggctt tttttggcgg agctggggcg ccctccggaa gcgtttccaa ctttccagaa 60 gtttctcggg acgggcagga gggggtgggg actgccatat atagatcccg ggagcagggg 120 agcgggctaa gagtagaatc gtgtcgcggc tcgagagcga gagtcacgtc ccggcgctag 180 cccagcccga cccaggccca ccgtggtgca cgcaaaccac ttcctggcca tgcgctccct 240 -77- 95238 .doc 1327645 cctgcttctc agcgccttct gcctcctgga ggcggccctg gccgccgagg tgaagaaacc 300 tgcagccgca gcagctcctg gcactgcgga gaagttgagc cccaaggcgg ccacgcttgc 360 cgagcgcagc gccggcctgg ccttcagctt gtaccaggcc atggccaag g accaggcagt 420 ggagaacatc ctggtgtcac ccgtggtggt ggcctcgtcg ctagggctcg tgtcgctggg 480 cggcaaggcg accacggcgt cgcaggccaa ggcagtgctg agcgccgagc agctgcgcga 540 cgaggaggtg cacgccggcc tgggcgagct gctgcgctca ctcagcaact ccacggcgcg 600 caacgtgacc tggaagctgg gcagccgact gtacggaccc agctcagtga gcttcgctga 660 tgacttcgtg cgcagcagca agcagcacta caactgcgag cactccaaga tcaacttccg 720 cgacaagcgc agcgcgctgc agtccatcaa cgagtgggcc gcgcagacca ccgacggcaa 780 gctgcccgag gtcaccaagg acgtggagcg cacggacggc gccctgctag tcaacgccat 840 gttcttcaag ccacactggg atgagaaatt ccaccacaag atggtggaca accgtggctt 900 catggtgact cggtcctata ccgtgggtgt catgatgatg caccggacag gcctctacaa 960 ctactacgac gacgagaagg aaaagctgca aatcgtggag atgcccctgg cccacaagct 1020 ctccagcctc atcatcctca tgccccatca cgtggagcct ctcgagcgcc ttgaaaagct 1080 gctaaccaaa gagcagctga agatctggat ggggaagatg cagaagaagg ctgttgccat 1140 ctccttgccc aagggtgtgg tggaggtgac ccatgacctg cagaaacacc tggctgggct 1200 gggcctgact gaggccattg acaagaacaa ggccgacttg tcacgcatgt caggcaagaa 126 0 ggacctgtac ctggccagcg tgttccacgc caccgccttt gagttggaca cagatggcaa 1320 cccctttgac caggacatct acgggcgcga ggagctgcgc agccccaagc tgttctacgc 1380 cgaccacccc ttcatcttcc tagtgcggga cacccaaagc ggctccctgc tattcattgg 1440 gcgcctggtc cggcctaagg gtgacaagat gcgagacgag ttatagggcc tcagggtgca 1500 cacaggatgg caggaggcat ccaaaggctc ctgagacaca tgggtgctat tggggttggg 1560 ggggaggtga ggtaccagcc ttggatactc catggggtgg gggtggaaaa acagaccggg 1620 gttcccgtgt gcctgagcgg accttcccag ctagaattpa ctccacttgg acatgggccc 1680 Cagataccat gatgctgagc ccggaaactc cacatcctgt gggacctggg ccatagtcat 1740 tctgcctgcc ctgaaagtcc cagatcaagc ctgcctcaat cagtattcat attiatagcc 1800 aggtaccttc tcacctgtga gaccaaattg agctaggggg gtcagccagc cctcttctga 1860 -78-

95238.doc 1327645 cactaaaaca cctcagctgc ctccccagct ctatcccaac ctctcccaac tataaaacta 1920 ggtgctgcag cccctgggac caggcacccc cagaatgacc tggccgcagt gaggcggatt 1980 gagaaggagc tcccaggagg ggcttctggg cagactctgg tcaagaagca tcgtgtctgg 2040 cgttgtgggg atgaactttt tgttttgttt cttccttttt tagttcttca aagataggga 2100 gggaaggggg aacatgagcc tttgttgcta tcaatccaag aacttatttg tacatttttt 2160 ttttcaataa aacttttcca atgacatttt gttggagcgt ggaaaaaa 2208 < 210 > 102 < 211 > 2566 < 212 > DNA < 213 > human < 400 > 102 ggcacgagtt gtgctcctcg cttgcctgtt ccttttccac gcattttcca ggataactgt 60 gactccaggc ccgcaatgga tgccctgcaa ctagcaaatt cggcttttgc cgttgatctg 120 ttcaaacaac tatgtgaaaa ggagccactg ggcaatgtcc tcttctctcc aatctgtctc 180 tccacctctc tgtcacttgc tcaagtgggt gctaaaggtg acactgcaaa tgaaattgga 240 caggttcttc attttgaaaa Tgtcaaagat ataccctttg gatttcaaac agtaacatcg 300 gatgtaaaca aacttagttc cttttactca ctgaaactaa tcaagcggct ctacgtagac 360 aaatctctga atctttctac agagttcatc agctctacga agagacccta tgcaaaggaa 4 20 ttggaaactg ttgacttcaa agataaattg gaagaaacga aaggtcagat caacaactca 480 attaaggatc tcacagatgg ccactttgag aacattttag ctgacaacag tgtgaacgac 540 cagaccaaaa tccttgtggt taatgctgcc lactttgttg gcaagtggat gaagaaattt 600 cctgaatcag aaacaaaaga at £ tcctttc agactcaaca agacagacac caaaccagtg 660 cagatgatga acatggaggc cacgttctgt atgggaaaca ttgacagtat caattgtaag 720 atcatagagc ttccttttca aaataagcat ctcagcatgt tcatcctact acccaaggat 780 gtggaggatg agtccacagg cttggagaag attgaaaaac aactcaactc agagtcactg 840 tcacagtgga ctaatcccag caccatggcc aatgccaagg tcaaactctc cattccaaaa 900 tttaaggtgg aaaagatgat tgatcccaag gcttgtctgg aaaatctagg gctgaaacat 960 atcttcagtg aagacacatc tgatuctct ggaatgtcag agaccaaggg agtggcccta 1020 -79- 95238.doc 1327645 tcaaatgtta tccacaaagt gtgcttagaa ataactgaag atggtgggga ttccatagag 1080 gtgccaggag cacggatcct gcagcacaag gatgaattga atgctgacca tccctttatt 1140 tacatcatca ggcacaacaa aactcgaaac atcattttct ttggcaaatt ctgttctcct 1200 taagtggcat agcccatgtt aagtcctccc tgacttttct gtggatgccg atttc tgtaa 1260 actctgcatc cagagattca ttttctagat acaataaatt gctaatgttg ctggatcagg 1320 aagccgccag tacttgtcat atgtagcctt cacacagata gacctttttt tttttccaat 1380 tctatctttt gtttcctttt ttcccataag acaatgacat acgcttttaa tgaaaaggaa 1440 tcacgttaga ggaaaaatat ttattcatta tttgtcaaat tgtccggggt agttggcaga 1500 aatacagtct tccacaaaga aaattcctat aaggaagatt tggaagctct tcttcccagc 1560 actatgcttt ccttctttgg gatagagaat gttccagaca ttctcgcttc cctgaaagac 1620 tgaagaaagt gtagtgcatg ggacccacga aactgccctg gctccagtga aacttgggca 1680 catgctcagg ctactatagg tccagaagtc cttatgttaa gccctggcag gcaggtgttt 1740 attaaaattc tgaattttgg ggattttcaa aagataatat tttacataca ctgtatgtta 1800 tagaacttca tggatcagat ctggggcagc aacctataaa tcaacacctt aatatgctgc 1860 aacaaaatgt agaatattca gacaaaatgg atacataaag actaagtagc ccataagggg 1920 tcaaaatttg ctgccaaatg cgtatgccac caacttacaa aaacacttcg ttcgcagagc 1980 ttttcagatt gtggaatgtt ggataaggaa ttatagacct ctagtagctg aaatgcaaga 2040 ccccaagagg aagttcagat cttaatataa attcactttc atttttgata gctgtcccat 2100 ctggtcatgt ggttggcact agactggtgg caggggcttc tagctgactc gcacagggat 2160 tctcacaata gccgatatca gaatttgtgt tgaaggaact tgtctcttca tctaatatga 2220 tagcgggaaa aggagaggaa actactgcct ttagaaaata taagtaaagt gattaaagtg 2280 ctcacgttac cttgacacat agtttttcag tctatgggtt tagttacttt agatggcaag 2340 catgtaactt atattaatag taatttgtaa agttgggtgg ataagctatc cctgttgccg 2400 gttcatggat tacttctcta taaaaaatat atatttacca aaaaattttg tgacattcct 2460 tctcccatct cttccttgac atgcattgta aataggttct tcttgttctg agattcaata 2520 Ttgaatttct cctatgctat tgacaataaa atattattga actacc 2566 <210> 103 -80·

95238.doc 1327645 < 211 > 2974 < 212 > DNA < 213 > human < 400 > 103 ctcagggcag agggaggaag gacagcagac cagacagtca cagcagcctt gacaaaacgt 60 tcctggaact caagctcttc tccacagagg aggacagagc agacagcaga gaccatggag 120 tctccctcgg cccctcccca cagatggtgc atcccctggc agaggctcct gctcacagcc 180 tcacttctaa ccttctggaa cccgcccacc actgccaagc tcactattga atccacgccg 240 ttcaatgtcg cagaggggaa ggaggtgctt ctacttgtcc acaatctgcc ccagcatctt 300 tttggctaca gctggtacaa aggtgaaaga gtggatggca accgtcaaat tataggatat 360 gtaataggaa ctcaacaagc taccccaggg cccgcataca gtggtcgaga gataatatac 420 cccaatgcat ccctgctgat ccagaacatc atccagaatg acacaggatt ctacacccta 480 cacgtcataa agtcagatct tgtgaatgaa gaagcaactg gccagttccg ggtatacccg 540 gagctgccca agccctccat ctccagcaac aactccaaac ccgtggagga caaggatgct 600 gtggccttca cctgtgaacc tgagactcag gacgcaacct acctgtggtg ggtaaacaat 660 cagagcctcc cggtcagtcc caggctgcag ctgtccaatg gcaacaggac cctcactcta 720 ttcaatgtca caagaaatga cacagcaagc tacaaatgtg aaacccagaa cccagtgagt 780 gccagg cgca gtgattcagt catcctgaat gtcctctatg gcccggatgc ccccaccatt 840 tcccctctaa acacatctta cagatcaggg gaaaatctga acctctcctg ccacgcagcc 900 tctaacccac ctgcacagta ctcttggttt gtcaatggga ctttccagca atccacccaa 960 gagctcttta tccccaacat cactgtgaat aatagtggat cctatacgtg ccaagcccat 1020 aactcagaca ctggcctcaa taggaccaca gtcacgacga tcacagtcta tgcagagcca 1080 cccaaaccct tcatcaccag caacaactcc aaccccgtgg aggatgagga tgctgtagcc 1140 ttaacctgtg aacctgagat tcagaacaca acctacctgt ggtgggtaaa taatcagagc 1200 ctcccggtca gtcccaggct gcagctgtcc aatgacaaca ggaccctcac tctactcagt 1260 gtcacaagga atgatgtagg accctatgag tgtggaatcc agaacgaatt aagtgttgac 1320 cacagcgacc cagtcatcct gaatgtcctc tatggcccag acgaccccac catttccccc 1380 tcatacacct attaccgtcc aggggtgaac ctcagcctct cctgccatgc agcctctaac 1440 ccacctgcac agtattcttg gctgattgat gggaacatcc agcaacacac acaagagctc 1500 -81 ·

95238.doc 1327645 tttatctcca acatcactga gaagaacagc ggactctata cctgccaggc caataactca 1560 gccagtggcc acagcaggac tacagtcaag acaatcacag tctctgcgga gctgcccaag 1620 ccctccatct ccagcaacaa ctccaaaccc gtggaggaca aggatgctgt ggccttcacc 1680 tgtgaacctg aggctcagaa cacaacctac ctgtggtggg taaatggtca gagcctccca 1740 gtcagtccca ggctgcagct gtccaatggc aacaggaccc tcactctatt caatgtcaca 1800 agaaatgacg caagagccta tgtatgtgga atccagaact cagtgagtgc aaaccgcagt 1860 gacccagtca ccctggatgt cctctatggg ccggacaccc ccatcatttc ccccccagac 1920 tcgtcttacc tttcgggagc gaacctcaac ctctcctgcc actcggcctc taacccatcc 1980 ccgcagtatt cttggcgtat caatgggata ccgcagcaac acacacaagt tctctttatc 2040 gccaaaatca cgccaaataa taacgggacc tatgcctgtt ttgtctctaa cttggctact 2100 ggccgcaata attccatagt caagagcatc acagtctctg catctggaac 2160 ctctcagctg gggccactgt cggcatcatg attggagtgc tggttggggt tgctctgata 2220 tagcagccct ggtgtagttt cttcatttca ggaagactga cagttgtttt gcttcttcct 2280 taaagcattt gcaacagcta cagtctaaaa ttgcttcttt accaaggata ttctcctggt Ttta cagaaa 2340 agactctgac cagagatcga gaccatccta gccaacatcg tgaaacccca tctctactaa 2400 aaatacaaaa atgagctggg cttggtggcg cgcacctgta gtcccagtta ctcgggaggc 2460 tgaggcagga gaatcgcttg aacccgggag gtggagattg cagtgagccc agatcgcacc 2520 actgcactcc agtctggcaa cagagcaaga ctccatctca aaaagaaaag aaaagaagac 2580 tctgacctgt actcttgaat acaagtttct gataccactg cactgtctga gaatttccaa 2640 aactttaatg aactaactga cagcttcatg aaactgtcca ccaagatcaa gcagagaaaa 2700 taattaattt catgggacta aatgaactaa tgaggattgc tgattcttta aatgtcttgt 2760 ttcccagatt tcaggaaact ttttttcttt taagctatcc actcttacag caatttgata 2820 aaaiatactt ttgtgaacaa aaattgagac atttacattt tctccctatg tggtcgctcc 2880 agacttggga aactattcat gaatatttat attgtatggt aatatagtta ttgcacaagt 2940 tcaataaaaa tctgctcttt gtataacaga aaaa 2974 < 210 > 104 < 211 > 3069 -82-

95238.doc 1327645 < 212 > DNA < 213 > human < 400 > 104 tgtttccgct gcatccagac ttcctcaggc ggtggctgga ggctgcgcat ctggggcttt 60 aaacatacaa agggattgcc aggacctgcg gcggcggcgg cggcggcggg ggctggggcg 120 cgggggccgg accatgagcc gctgagccgg gcaaacccca ggccaccgag ccagcggacc 180 ctcggagcgc agccctgcgc cgcggaccag gctccaacca ggcggcgagg cggccacacg 240 caccgagcca gcgacccccg ggcgacgcgc ggggccaggg agcgctacga tggaggcgct 300 aatggcccgg ggcgcgctca cgggtcccct gagggcgctc tgtctcctgg gctgcctgct 360 gagccacgcc gccgccgcgc cgtcgcccat catcaagttc cccggcgatg tcgcccccaa 420 aacggacaaa gagttggcag tgcaatacct gaacaccttc tatggctgcc ccaaggagag 480 ctgcaacctg tttgtgctga aggacacact aaagaagatg cagaagttct ttggact £ cc 540 ccagacaggt gatcttgacc agaataccat cgagaccatg cggaagccac gctgcggcaa 600 cccagatgtg gccaactaca acttcttccc tcgcaagccc aagtgggaca agaaccagat 660 cacatacagg atcattggct Acacacccga tctggaccca gagacagtgg atgatgcctt 720 tgctcgtgcc ttccaagtct ggagcgatgt gaccccactg cggttttrtc gaatccatga 780 tggagaggca gacatcatga tcaactttgg ccgctgggag catggcgatg gatacccctt 840 tgacggtaag gacggactcc tggctcatgc cttcgcccca ggcactggtg ttgggggaga 900 ctcccatttt gatgacgatg agctatggac cttgggagaa ggccaagtgg tccgtgtgaa 960 gtatggcaac gccgatgggg agtactgcaa gttccccttc ttgttcaatg gcaaggagta 1020 caacagctgc actgatactg gccgcagcga tggcttcctc tggtgctcca ccacctacaa 1080 ctttgagaag gatggcaagt acggcttctg tccccatgaa gccctgttca ccatgggcgg 1140 caacgctgaa ggacagccct gcaagtttcc attccgcttc cagggcacat cctatgacag 1200 ctgcaccact gagggccgca cggatggcta ccgctggtgc ggcaccactg aggactacga 1260 ccgcgacaag aagtatggct tctgccctga gaccgccatg tccactgttg gtgggaactc 1320 agaaggtgcc ccctgtgtct tccccttcac tttcctgggc aacaaatatg agagctgcac 1380 cagcgccggc cgcagtgacg gaaagatgtg gtgtgcgacc acagccaact acgatgacga 1440 ccgcaagtgg ggcttctgcc ctgaccaagg gtacagcctg ttcctcgtgg cagcccacga 1500 -83-

95238.doc 1327645 gtttggccac gccatggggc tggagcactc ccaagaccct ggggccctga tggcacccat 1560 ttacacctac accaagaact ccaggatgac atcaagggca ttcaggagct 1620 ctatggggcc tctcctgaca ttgaccttgg caccggcccc acccccacac tgggccctgt 1680 cactcctgag atctgcaaac aggacattgt atttgatggc atcgctcaga tccgtggtga 1740 gatcttcttc ttcaaggacc ggttcatttg gcggactgtg acgccacgtg acaagcccat 1800 ggggcccctg ctggtggcca cattctggcc tgagctcccg gaaaagattg atgcggtata 1860 cgaggcccca caggaggaga aggctgtgtt ctttgcaggg tccgtctgtc aatgaatact ggatctactc 1920 agccagcacc ctggagcgag ggtaccccaa gccactgacc agcctgggac tgccccctga 1980 tgtccagcga gtggatgccg cctttaactg gagcaaaaac aagaagacat acatctttgc 2040 tggagacaaa ttctggagat acaatgaggt gaagaagaaa atggatcctg gctttcccaa 2100 gctcatcgca gatgcctgga atgccatccc cgataacctg gatgccgtcg tggacctgca 2160 gggcggcggt cacagctact tcttcaaggg tgcctattac ctgaagctgg agaaccaaag 2220 tctgaagagc gtgaagtttg gaagcatcaa atccgactgg ctaggctgct gagctggccc 2280 tggctcccac aggcccttcc tctccactgc cttcgataca ccgggcctgg Gaga ctagag 2340 aaggacccgg aggggcctgg cagccgtgcc ttcagctcta cagctaatca gcattctcac 2400 tcctacctgg taatttaaga ttccagagag tggctcctcc cggtgcccaa gaatagatgc 2460 tgactgtact cctcccaggc gccccttccc cctccaatcc caccaaccct cagagccacc 2520 cctaaagaga tcctttgata ttttcaacgc agccctgctt tgggctgccc tggtgctgcc 2580 acacttcagg ctcttctcct ttcacaacct tctgtggctc acagaaccct tggagccaat 2640 ggagactgtc tcaagagggc actggtggcc cgacagcctg gcacagggca gtgggacagg 2700 gcatggccag gtggccactc cagacccctg gcttttcact gctggctgcc ttagaacctt 2760 tcttacatta gcagtttgct ttgtatgcac tttgtttttt tctttgggtc ttgttttttt 2820 tttccactta gaaattgcat ttcctgacag aaggactcag gttgtctgaa gtcactgcac 2880 agtgcatctc agcccacata gtgatggttc ccctgttcac tclactlagc atgtccctac 2940 cgagtctctt ctccactgga tggaggaaaa ccaagccgtg gcttcccgct cagccctccc 3000 tgcccctccc ttcaaccatt ccccatggga aatgtcaaca agtatgaata aagacaccta 3060 ctgagtggc 3069 84 ·

95238.doc 1327645 < 210 > 105 < 211 > 3299 < 212 > DNA < 213 > human < 400 > 105 cggagggagc gctgggagcg agcaagcgag cgtttggagc ccgggccagc agagggggcg 60 cccggtcgct gcctgtaccg ctcccgctgg tcatctccgc cgcgctcggg ggccccggga 120 ggagcgagac cgagtcggag agtccgggag ccaagccggg cgaaacccaa ctgcggagsa 180 cgcccgcccc actcagcctc ctcctgcgtc cgagccgggg agcatcgccg agcgccccac 240 gggccggaga gctgggagca caggtcccgg cagccccagg gatggtctag gagccggcgt 300 aaggctcgct gctctgctcc ctgccggggc tagccgcctc ctgccgatcg cccggggctg 360 cgagctgcgg cggcccgggg ctgctcgccg ggcggcgcag gccggagaag ttagttgtgc 420 gcgcccttag tgcgcggaac cagccagcga gcgagggagc agcgaggcgc cgggaccatg 480 ggctggggga gccgctgctg ctgcccggga cgtttggacc tgctgtgcgt gctggcgctg 540 ctcgggggct gcctgctccc cgtgtgtcgg acgcgcgtct acaccaacca ctgggcagtc 600 aaaatcgccg Ggggcttccc ggaggccaac cgtatcgcca gcaagtacgg attcatcaac 660 ataggacaga taggggccct gaaggactac taccacttct accatagcag gacgattaaa 720 aggtcagtta tctcgagcag agggacccac agtttcattt caatggaacc aaagg tggaa 780 tggatccaac agcaagtggt aaaaaagcgg acaaagaggg attatgactt cagtcgtgcc 840 cagtctacct atttcaatga tcccaagtgg cccagcatgt ggtatatgca ctgcagtgac 900 aatacacatc cctgccagtc tgacatgaat atcgaaggag cctggaagag aggctacacg 960 ggaaagaaca ttgtggtcac tatcctggat gacggaattg agagaaccca tccagatctg 1020 atgcaaaact acgatgctct ggcaagttgc gacgtgaatg ggaatgactt ggacccaatg 1080 cctcgttatg atgcaagcaa cgagaacaag catgggactc gctgtgctgg agaagtggca 1140 gccgctgcaa acaattcgca ctgcacagtc ggaattgctt tcaacgccaa gatcggagga 1200 gtgcgaatgc tggacgga £ a tgtcacggac atggttgaag caaaatcagt tagcttcaac 1260 ccccagcacg tgcacattta cagcgccagc tggggcccgg atgatgatgg caagactgtg 1320 gacggaccag cccccctcac ccggcaagcc tttgaaaacg gcgttagaat ggggcggaga 1380 ggcctcggct ctgtgtttgt ttgggcatct ggaaatggtg gaaggagcaa agaccactgc 1440 -85- 95238.doc 1327645 tcctgtgatg gctacaccaa cagcatctac accatctcca tcagcagcac tgcagaaagc 1500 ggaaagaaac cttggtacct ggaagagtgt tcatccacgc tggccacaac Ctacagcagc 1560 ggggastcct acgataagaa aatcatcact acagatctga gg cagcgttg cacggacaac 1620 cacactggga cgtcagcctc agcccccatg gctgcaggca tcattgcgct ggccctggaa 1680 gccaatccgt ttctgacctg gagagacgta cagcatgtta ttgtcaggac ttcccgtgcg 1740 ggacatttga acgctaatga ctggaaaacc aatgctgctg gttttaaggt gagccatctt 1800 tatggatttg gactgatgga cgcagaagcc atggtgatgg aggcagagaa gtggaccacc 1860 gttccccggc agcacgtgtg tgtggagagc acagaccgac aaatcaagac aatccgccct 1920 aacagtgcag tgcgctccat ctacaaagct tcaggctgct cggataaccc caaccgccat 1980 gtcaactacc tggagcacgt cgttgtgcgc atcaccatca cccaccccag gagaggagac 2040 ctggccatct acctgacctc gccctctgga actaggtctc agcttttggc caacaggcta 2100 tttgatcact ccatggaagg attcaaaaac tgggagttca tgaccattca ttgctgggga 2160 gaaagagctg ctggtgactg ggtccttgaa gtttatgata ctccctctca gctaaggaac 2220 tttaagactc caggtaaatt gaaagaatgg tctttggtcc tctacggcac ctccgtgcag 2280 ccatattcac caaccaatga atttccgaaa gtggaacggt tccgctatag ccgagttgaa 2340 gaccccacag acgactatgg cacagaggat tatgcaggtc cctgcgaccc tgagtgcagt 2400 gaggttggct gtgacgggcc aggaccagac cactgcaatg actgtttg ca ctactactac 2460 aagctgaaaa acaataccag gatctgtgtc tccagctgcc cccctggcca ctaccacgcc 2520 gacaagaagc gctgcaggaa gtgtgccccc aactgtgagt cctgctttgg gagccatggt 2580 gaccaatgca tgtcctgcaa atatggatac tttctgaatg aagaaaccaa cagctgtgtt 2640 actcactgcc ctgatgggtc atatcaggat accaagaaaa atctttgccg gaaatgcagt 2700 gaaaactgca agacatgtac tgaattccat aactgtacag aatgtaggga tgggttaagc 2760 ctgcagggat cccggtgctc tgtctcctgt gaagatggac ggtatttcaa cggccaggac 2820 tgccagccct gccaccgctt ctgcgccact tgtgctgggg caggagctga Tgggtgcatt 2880 aactgcacag agggctactt catggaggat gggagatgcg tgcagagctg tagtatcagc 2940 tattactttg accactcttc agagaatgga tacaaatcct gcaaaaaatg tgatatcagt 3000 tgtttgacgt gcaatggccc aggattcaag aactgtacaa gctgccctag tgggtatctc 3060 -86-

95238.doc 1327645 ttagacttag gaatgtgtca aatgggagcc atttgcaagg atgcaacgga agagtcctgg 3120 gcggaaggag gcttctgtat gcttgtgaaa aagaacaatc tgtgccaacg gaaggttctt 3180 caacaacttt gctgcaaaac atgtacattt caaggctgag cagccatctt agatttcttt 3240 gttcctgtag acttatagat tattccatat tattaaaaag aaaaaaaaaa gccaaaaag 3299 < 210> 106 < 211 > 1664 < 2i2 > DNA < 213 > human < 400 > 106 atgggttgtg actgcttcgt ccaggaggtg ttctgctcag atgaggagct tgccaccgtc 60 ccgctggaca tcccgccata tacgaaaaac atcatctttg tggagacctc gttcaccaca 120 ttggaaacca gagcttttgg cagtaacccc aacttgacca aggtggtctt cctcaacact 180 cagctctgcc agtttaggcc ggatgccttt ggggggctgc ccaggctgga ggacctggag 240 gtcacaggca gtagcttctt gaacctcagc accaacatct tctccaacct gacctcgctg 300 ggcaagctca ccctcaactt caacatgctg gaggctctgc ccgagggtct tttccagcac 360 ctggctgccc Tggagtccct ccacctgcag gggaaccagc tccaggccct gcccaggagg 420 ctcttccagc ctctgaccca tctgaagaca ctcaacctgg cccagaacct cctggcccag 480 ctcccggagg agctgttcca cccactcacc agcctgcaga ccctgaagct ga gcaacaac 540 gcgctctctg gtctccccca gggtgtgttt ggcaaactgg gcagcctgca ggagctcttc 600 ctggacagca acaacatctc ggagctgccc cctcaggtgt tctcccagct cttctgccta 660 gagaggctgt ggctgcaacg caacgccatc acgcacctgc cgctctccat ctttgcctcc 720 ctgggtaatc tgacctttct gagcttgcag tggaacatgc ttcgggtcct gcctgccggc 780 ctctttgccc acaccccatg cctggttggc ctgtctctga cccataacca gctggagact 840 gtcgctgagg gcacctttgc ccacctgtcc aacctgcgtt ccctcatgct ctcatacaat 900 gccattaccc acctcccagc tggcatcttc agagacctgg aggagttggt caaactctac 960 ctgggcagca acaaccttac ggcgctgcac ccagccctct tccagaacct gtccaagctg 1020 gagctgctca gcctctccaa gaaccagctg accacacttc cggagggcat cttcgacacc 1080 aactacaacc tgttcaacct ggccctgcac ggtaacccct ggcagtgcga ctgccacctg 1140 -87-

95238.doc 1327645 gcctacctct tcaactggct gcagcagtac accgatcggc tcctgaacat ccagacctac 1200 tgcgctggcc ctgcctacct caaaggccag gtggtgcccg ccttgaatga gaagcagctg 1260 £ tgtgtcccg tcacccggga ccacttgggc ttccaggtca cgtggccgga cgaaagcaag 1320 gcagggggca gctgggatct ggctgtgcag gaaagggcag cccggagcca gtgcacctac 1380 agcaaccccg agggcaccgt ggtgctcgcc tgtgaccagg cccagtgtcg ctggctgaac 1440 gtccagctct ctcctt £ gca gggctccctg ggactgcagt acaatgctag tcaggagtgg 1500 gacctgaggt cgagctgcgg ttctctgcgg ctcaccgtgt ctatcgaggc tcgggcagca 1560 gggccctagt agcagcgcat acaggagctg gggaaggggg ctttggggcc tgcccacgcg 1620 acaggtaggg gcggagggga gctgagtctc cgaagcttgg cttt 1664 < 210 > 107 < 211 > 3383 < 212 > DNA < 213 > human < 400> 107 cgggggccgc gcgggcaaga tggtgtgcgc tcgggcggcc ctcggtcccg gcgcgctctg 60 ggccgcggcc tggggcgtcc tgctgctcac agcccctgcg ggggcgcagc gtggccggaa 120 gaaggtcgtg cacgtgctgg agggtgagtc gggctcggta gtggtacaga cagcgcctgg 180 gcaggtggta agccaccgtg gtggcaccat cgtcttgccc tgccgctacc actatgaggc 2 40 agccgcccac ggtcacgacg gcgtccggct caagtggaca aaggtggtgg acccgctggc 300 cttcaccgac gtcttcgtgg cactaggccc ccagcaccgg gcattcggca gctaccgtgg 360 gcgggctgag ctgcagggcg acgggcctgg ggatgcctcc ctggtcctcc gcaacgtcac 420 gctgcaagac tacgggcgct atgagtgcga agtcaccaat gagctggaag atgacgctgg 480 catggtcaag ctggacctgg aaggcgtggt ctttccctac cacccccgtg gaggccgata 540 caagctgacc ttcgcggagg cgcagcgcgc gtgcgccgag caggacggca tcctggcatc 600 tgcagaacag ctgcacgcgg cctggcgcga cggcctggac tggtgcaacg cgggctggtt 660 gcgcgacggc tcagtgcaat accccgtgaa ccggccccgg gagccctgcg gcggcctggg 720 ggggaccggg agtgcagggg gcggcggtga tgccaacggg ggcctgcgca actacgggta 780 tcgccataac gccgaggaac gctacgacgc cttctgcttc acgtccaacc tgccggggcg 840 cgtgttcttc ctgaagccgc tgcgacctgt acccttctcc ggagctgcgc gcgcgtgtgc 900 • 88 ·

95238.doc 1327645 tgcgcgtggc gcggccgtgg ccaaggtggg gcagctgttc gccgcgtgga agctgcagct 960 gctagaccgc tgcaccgcgg gttggctggc cgatggcagt gcgcgctacc ccatcgtgaa 1020 cccgcgagcg cgctgcggag gccgcaggcc tggtgtgcgc agcctcggct tcccggacgc 1080 cacccgacgg ctcttcggcg tctactgcta ccgcgctcca ggagcaccgg acccggcacc 1140 tggcggctgg ggctggggct gggcgggcgg cggcggctgg gcagggggcg cgcgcgatcc 1200 tgctgcctgg acccctctgc acgtctaggc tgggagtagg cggacagcca gggcgcttga 1260 ccactggtct agagccctgt ggtcccctgg agcctggcca cgcccttgaa gccctggaca 1320 ctggccacat tccctgtggt cccttacaaa ctaactgtgc ccctggggtc cctgaagact 1380 ggctagtcct ggcagaacag tactttggag ttccctggag cctggccagc cctcacctct 1440 tctggataga ggattccccc aactccccaa ctttctccat gagggtcacg ccccctgagg 1500 acctcaggag gccagcagaa cccgcaggct cctgaagact ggccacgcct cctgagacca 1560 cttggaaaca gaccaactgc ccccgtggtc gcctggtggc tggacccccg ggattgacta 1620 gagaccggcc gtacaccttc tgcatctcac tggagactga acactagtcc cttgcggtca 1680 cgtgggacac tgggcgcctc ctcctccccc tcctcctcac ctggagagac Tacag gaact 1740 tcagggtcac tccccgtggt cacatggagg ttgtgggccg aggcgcttat tttcccttat 1800 ggtgacctga gtcctggaga ctcccattct ccccctctcc ctgagagtcc cctgcagttt 1860 ctgggtaaca gggcacaccc ctctagtttc atgggcgagc acccccatct gccacctcag 1920 actgacacac agccagctgg ctcacttact gggggccacg tcccacccct cagatatttc 1980 tttgaaggga gagcaaaccc accctgtcct ctgacgtccc tttcccaact gtcaccaaac 2040 agaccatctt cccaggcctg gggaccggta agatccatgt cactagttat gcagagcagt 2100 tgccttgggt cccactgtca ccaaggcaac cagtcctgct gctacctgtc acctagagtc 2160 acacacccct tccctcatca ggcacaccca tgaagacagt gcctccctcc tccagctgta 2220 accatggata ccacacattt ctcatctcat tggcccccac cccagagacc tccacctcaa 2280 cttctggctg tccctaccct gactcaccgc catggagatc accctccccg aagctgtcgc 2340 cagggtgacc caacatccag ttctccggct ctcaccatgg aaacaaactg tccctgtccc 2400 caggcccact ccagttccag accaccctcc atgctccacc cccaggcggt ttggacccca 2460 ccactgttgc catggtgacc aaactctgga gtccgaggta acagaacacc tgtcccccta 2520 -89-

95238.doc 1327645 ggcttttcct tgtggacaac ggggccctgt tcaccaagct gttgccatag agactgtcaa 2580 cgttgtcctc atgacaacca gacttccagUctcaggaac ttctcattgt gggccagaag 2640 tcctgggtgc ctcctactag ggctacccta ctgcacccca tcaggggcct gatggctgcc 2700 ccttccccag acagggctgg acttctggag ctgctaagcc accctccgtt tgcacgttaa 2760 ctctatgccg gatagcagct gtgcacgaga caatcttgca acacccgggc atgtttgtcg 2820 tcgtcctaca aatgaggaaa ccgagcctat ggcgtgccct ggtctgttga gatatgcaag 2880 cactgagctc ctcttttgtc ctctgagacc ccatctccat tctcacccag ttcctctctc 2940 cttccctgac ccccacccac atttccctcc ttagagatcc aggagggatg gaatgttctt 3000 taaaattcaa cacccaccag gctctaagcg gcgatctgtg ctaagaggtc aggacccagc 3060 cgaagtcctc ggcgttgaca ggcagctggg gggacatgat ccatggacaa ggccatcccg 3120 gccgtgggag accccagtcc cgaagtcttg cctgcaggag tactggggtc cccctggggc 3180 cctcttlact gtcacgtcat ctclaggaaa cctatctctg agttttggga ccaggtcggt 3240 ttgggtttga attctgcctc ttcttgctra ctgtgtgacc aagtgacaaa ctccttctga 3300 acctgtgttc tcccactgta ccagggctgt tctgtggtcc ccgtgagtgc caagca Taca 3360 gtaggggctc aataaatcct tgt 3383 <210> 108 <211> 17 <212>protein <213> human <400>

Phe Ala lie Ser Glu Tyr Asn Lys Ala Thr Lys Asp Asp Tyr Tyr Arg 1 5 10 15

Arg -90- 95238.doc

Claims (1)

1327645 Patent Application No. 093124387 Replacement of Chinese Patent Application (April 1999) X. Patent Application Range: 1. A method for detecting gastric cancer, comprising: (a) providing a biological sample; and (b) Excessive performance of a member of the gastric tumor marker (GTM) family is detected in a sample, wherein the GTM family member is a cysteine protease inhibitor SN ("CSTl"). 2. The method of claim 1, which includes the following Further steps: comparing the amount of GTM present in the test sample to the value obtained from a control sample obtained from an individual not having gastric cancer. 3. The method of claim 1, which includes detecting Further overexpression of at least one member of the GTM family selected from the group consisting of carboxypeptidase N, polypeptide 2, 83 kDa chain (CPN2), matrix metalloproteinase 12 (MMP12), statin ("INHBA"), Insulin-like growth factor 7 ("IGFBP7"), γ-glutamine hydrolase ("GGH"), proteoglycan-rich proteoglycan ("LEPRE1"), cysteine protease inhibition Agent S ("CST4"), Bleeding-related protein 4 ("SFRP4"), Aspen ("ASPN"), cell growth regulator with EF hand function site 1 ("CGREF 1"), kallikrein 10 (nKLK10" ), tissue inhibitor of metalloproteinase 1 ("TIMP1"), secreted acidic cysteine-rich protein ('SPARC'), transforming growth factor, β-induced ("TGFBI"), Containing EGF, such as phenanthrene extracellular matrix protein 2 ("EFEMP2"), luminol ("LUM), sternin ("SNN"), secretory phosphoprotein l ("SPPln) Chondroitin sulfate proteoglycan 2 ("CSPG2"), N-mercaptosphingosine guanamine hydrolase ("ASAH1"), serine protease 11 (''PRSS11'), secretory [s] 95238- 990408.doc Curl-related protein 2 ("SFRP2"), phospholipase A2 'XIIB group ("PLA2G12B"), statin 2, extracellular matrix protein ("SP0N2··), olmolemidine 1 ("0LFM1··), a thrombospondin repeat containing 1 ("TSRC1"), thrombospondin 2 (" THBS2"), Adelikon, cysteine protease inhibitor SA ("CST2"), adenine oxidase-like enzyme 2 ("L0XL2"), thyroglobulin ("TG"), Transforming growth factor beta ("TGFB1"), serine or cysteine protease inhibitor, grade H ("SERPINH1"), serine or cysteine protease inhibitor, grade B ("SERPINB5 "), matrix metalloproteinase 2 ("ΜΜΡ2"), proprotein convertase subtilisin/Kaixin type 5 ("PCSK5'') and hyaluronan glycoprotein-linked protein 4 ("HAPLN4"). 4. The method of claim 3, wherein the at least one other GTM member is selected from the group consisting of ampoule oxidase-like enzyme 2 ("L0XL2"), secretory coil-related protein 2 ("SFRP2"), silk Aminic acid or cysteine protein inhibitor, level H ("SERPINH1") and a serine or cysteine protein inhibitor, level B ("SERPINB5") 5. As in the method of claim 1, Wherein step (b) comprises detecting an overexpression of at least one member of the GTM family selected from the group consisting of: amidoxime oxidase-like enzyme 2 ("L0XL2)), secretory coil-related protein 2 ( &quot ;SFRP2"), a serine or cysteine protein inhibitor, level H ("SERPINH1") and a serine or cysteine protein inhibitor, level B ("SERPINB5"). The method of any one of claims 1 to 5, wherein the detecting step is performed by detecting the excessive expression of the GTM mRNA. The method of any one of items 1 to 5, wherein the method of any one of items 1 to 5, wherein The test was performed by detecting excessive expression of GTm cDNA. The method of monthly term 7 wherein the detection step is carried out using an oligonucleotide complementary to at least one of the GTM cDNAs. 9. The method of claim 7, wherein the qPCR method is used, using The method of any one of the items 1 to 5, wherein the detecting step is performed by detecting excessive expression of the protein shell. η·such as any one of the items (1) The method of the present invention, wherein the detecting step is performed by detecting the over-expression of the (four) shape, such as the method of any one of clauses 1 to 5, wherein the detecting step is performed using an antibody against the G-top. - a type of immunoassay 13. The method of detecting is carried out according to the method of claim 12. 14. 15. 16. The method of claim 13 wherein the antibody is a monoclonal antibody, as in the method of claim 13, such as a request Any one of items 1 to 5 is analyzed by ELISA, wherein the antibody is a plurality of antiserums. The method of claim, wherein the detecting step is such that the sample to be tested is obtained from the sample to be tested. Item 至 to any of 5 The method of self-organizing, urinary, gastric juice, serum and feces. The method of detecting gastric cancer, comprising: 95238-990408.doc 1327645 There are two or more GTM capture reagents on the substrate, which can be combined with GTM cysteine protease inhibitor SN ("CST1") and further one or more GTMs selected from the group consisting of:醯 oxidase-like enzyme 2 ("LOXL2"), secreted curl-related protein 2 ("SFRP2"), serine or cysteine protein inhibitor, grade Η ("SERPINH1") and silk Amine acid or cysteine protein inhibitor, level B ("SERPINB5"); a tool for coloring the GTM capture reagent and GTM complex; reagent; and instructions for use. 20. The kit of claim 19, wherein the GTM capture reagent is a GTM-specific oligonucleotide. 21. The kit of claim 19, wherein the GTM capture reagent is a GTM-specific antibody that is selective for a GTM oligonucleotide, a GTM protein, or a GTM peptide. 95238-990408.doc 1327645 Patent No. 93124387 Patent Application Replacement Page (May 1998) Η一,图: Correction of replacement of the scorpion-type transforming growth factor, (3-square-guided | osteogenic protein |Human Inhibin PA 健 | Secreted 棬曲相 W Protein 4 丨 contains egf · 菲布林 extracellular matrix protein 2 1 | Adelikon | Human anemia or + cysteine protease inhibitor, P& 基质 1 Matrix Metalloproteinase 2 1 Human Proprotein Transfer® Enzyme Bacillus subtilis/Kaixin 53⁄4 1 Transforming Growth Factor pi | Human Serine or Cysteine Protease Inhibitor • Location | Human cell growth regulator for hand function 1 1 thyroglobulin I | thrombospondin 2 1 fibroin protease 11 (IGF binding) | I secreted protein, acidic * rich in cysteamine | Secretory curl-associated protein 2 1 |N- sister sphingosine-peptidylamine hydrolase 1 I metalloproteinase inhibitor i 1 | Matrix gold-chip protease 12 1 | Amine δδ oxidase-class enzyme 2 1 1 Rumiken 1 13⁄4 proteoglycans containing leucine amidoxime i (in Puken l) | 1 kallikrein ίο | | class Island growth factor binding protein 7 1 | γ · guanamine aa hydrolase 1 1 cysteamine chymotrypsin inhibitor SN, SA & S 1 | thiopurine chondroitin protein multipolar 2 (Foxon) | | Aspo® ( Lrr Dijon) | Brother | TGFB1 | SPP1 丨ΓΝΗΒ 1 1 1 SFRP4 1 EFEMF'2 SERPINH1 | MMP2 1 PCSK5 | TGFB1 | SERPINI15 1 CGREFil 〇| THBS2 ] 1 PRSS11 1 | SPARC 1 1 SFRP2 1 1 ASAH1 1 1 TIMP1 1 1 MMP12 1 1 L0XL2 1 | LUM 1 | LEPRE1 1 | KLK10 1 | IGFBP7 1 1 GGH 1 1 CST1,2,4 1 | CSPG2 1 | ASPN 1 Symbol | Hs00165908_ml | Hs00167093_ml 1 1 Hs00170103_ml | 1 Hs00180066_ml | | Hs00213545_ml | Hs00377849_ml | Hs〇0241844_ml ΜΦ-M g (Β ^ ITTGATGGCATCGCTCAGATC | AAAAATCTTTGCCGGAAATGC [GGTCCATGTCATCACCAATGTT | TCCACGCATTTTCCAGGATAA | CTGCCC ACCCCTTCC A | | GACGGTTCCTCGC AGTTC AA | ITGGAAGGACTACACGGCCTATAG | Itcgggaggcccgttagtaa I Itcttccctgtacactggcagttc I 1CGCTAGC AGCG ACCACCT | ICGCAGAACGCCTGCAAA | 1CCAGACCACCTTATACCAGCG | Igcctctctgctgatgacatacgt I Iaggccagcttctgcttgga I iGATTCTTGTCCATAGTGA TCTGC | iCTTGAGTACAACGCTGACCTCTTC | IACAACATGATATGTGCTGGACTGG | CAGGTCAGCAAGGGCACC | GTGGCAATGCCGCTGAA | AGTCCCAGCCCAACTTGGA | GCCAGTGGAATGATGTTCCC | AAATACAAAAGGACACATTCAAAGGA | forward primer N) 〇〇- 5: two square μ two s NO oe \ u > LO - SEQ ID ITGTCACGTGGCGTCACAGT | AGTCCTGGCCGTTGAAATACC ITCTGCAAGTTCATCCCCTCTTT | AAGCCGAATTTGCTAGTTGCA Ittctgtccttcctagtccctttagg I Itgtaaaccgctccacttcacat 1 | TAGGTTTGGTCATAGATAGGTCCTGAGT | Iaaggagattccagctgtcactttc I 1GAAAAAGCGGGTGGTGCA | Ittttgcaggcttcacataccttt I Iacaggacatcatacatggtttcaaa I Iggacctgtggaagtatccgc I Iagtgacagcatcaaaactcaaattg I ICCCTGATCGCCGAGTTG | ICCAATCAATGCCAGGAAGAGA] ICCGTGACACAGTTCTGCTTACAG] IGAGAGGATGCCTTGGAGGGT | | TCACAGCTCAAGTACACCTGGG | TGACAGCAACAACTCAGTAGGAAAA | IGGGAACTTCGTAGATCTGGAAAGA | [TCTTGGCATTTTCTACAACAGGG | ITGCTTCTGCAATTCTGATATGGA | reverse primer OJ > 〇90 LO u > OJ U) u >N>u> to 00 K> IO ΰ 辑 |ϊ 21 This ITTCAAGGACCGGTTCATT TGGCG | ACAGAATGTAGGGATGGGTTAAGCCTGCA | CAGCCTCCAGCCAACAGACCTCAGG | TGACTCCAGGCCCGCAATGGA ICCAGGCCAGGAGCAGCTCGG | ITCTGGCAGATTCCGATGCCCCACAA | Iaggcccaagaccggctacatcagagtc I lAGTGTTAATTCCAATCACTTCACCGTCCAGG | ITGGACCAGCACCCCATTGACGG | ICTGCCAGCCACCGAGGAAGCTC | ITGTCTGAACCGCACCAGCCAAGAGAATA | ICAAGATGACCAAGATGTATAAAGGGTTCCAAGC ITCAGTCCCTGTATGGAGACCCAAAAGAGAA; ICGTAATTCTTCTGGATGTCTCCTTCACATTCTG ITAAGGATTCAAACCATTTGCCAAAAATGAGTCTAAG ICCATCACAGATCATTACATCCAGGTCCTCA ICTTGCCAGAGTGACTCTGGAGGCCC IAGCAAGGTCCTTCCATAGTGACGCCC ITTCACTGGAGGTCAATTGCACAGCAGAAT lAGCCAGAACTGCAGAAGAAACAGTTGTGC IAGGAACAGTTGCTTGCGGCCAGC | TTGGAAATGAGTGCAAACCCTCTTGATAATAATG retention pin S:! 2 square »s Ό 00 Ln K > e: 0 ^ m ^ 95238.doc 1327645 Patent Application No. 093124387 Replacement Page of Chinese Patterns (May 1998) Whipping Qing Day Correction Replacement Page 命对一卜'"'-铕冲铋申||3-$薛甶 鸯冷? 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