1322819 六、發明說明： 【發明所屬之技術領域】 本發明係有關於胜肽化合物，特別是有關於對胺類具 有高鍵結敏感度和鍵結強度，可用於檢測胺類化合物之胜 肽化合物。本發明亦有關於利用上述胜肽化合物檢測胺類 化合物之方法。 【先前技術】 在哺乳動物的嗅覺系統中，位於鼻黏膜上具有嗅覺接 受蛋白嵌於細胞膜上，此嗅覺接受蛋白可以與氣體分子反 應後，將訊息經由一連串的生化反應傳至大腦的嗅覺區判 斷味道種類。嗅覺接受蛋白對不同的氣體分子具有不同的 鍵結位置，此完全取決於其氨基酸序列與所形成之立體結 構；本發明係以此為契子進而推論不同的胜肽序列對不同 的氣體分子或化合物具有不同的鍵結能力。即配合感測 器，設計不同的胜肽作為接受膜，而達到檢測特定化合物 之目的。 習知技術中相關專利如US 6,218,507，是利用合成的 胜肽檢測三曱基胺，惟其胜肽序列與本發明完全不同。 在許多疾病狀態下，如腎臟病、尿毒症、肝病、胃潰 瘍等情況，體内的胺類化合物會偏高，因此可藉由測量患 者呼氣中所含之胺類化合物的含量以判斷是否有此類疾 病。另外，腐壞的海產如魚類、貝類亦會釋放出胺類化合 物，因此本發明亦可應用於海產新鮮度的檢測。相同地， 本發明亦適用於空氣或水中胺類化合物含量之檢測，進而 1322819 判斷是否有空氣污染或水質污染的情況。 【發明内容】 本發明提供一種胜肽化合物，其對胺類化合物具有高 敏感度及高鍵結強度，適用於胺類化合物之檢測。 本發明亦提供以上述胜肽化合物檢測胺類化合物之方 法，包括下列步驟：令一待測物質與一感測元件接觸，此 感測元件上覆被有前述之胜肽化合物；待測物質與感測元 件反應所得之信號；以及將信號與一資料庫比對，以定義 待測物質中的胺類化合物含量。 本發明亦提供一檢測胺類之裝置，包括一感測元件， 其上覆被有前文所述胜肽化合物，及一信號處理單元耦接 於該感測元件用以產生一信號。 本發明之胜肽序列對於胺類化合物具有高敏感度及高 鍵結強度的特性，利用此胜肽序列或含有此胜肽之衍生物 作為與嗅覺閥值低的胺類化合物反應或檢測的物質，運用 於感測器的檢測上，作為檢測胺類化合物之工具，具有提 高敏感度及低濃度物質檢測適用性等特質。 為了讓本發明之上述和其他目的、特徵、和優點能更 明顯易懂，下文特舉較佳實施例，並配合所附圖示，作詳 細說明如下： 【實施方式】 為達以設計胜肽檢測胺類化合物之目的，可經由蛋白 1322819 質之二級結構分析胜肽序列特性或利用蛋白質之三級結構 模擬與目標分子之鍵結位置或根據檢測物質之物性與化性 任意設計氨基酸之組合，進行胜肽序列設計，再以人工方 式加以合成；所得到之胜肽化合物配合感測器或晶片將訊 號輸出，可用於胺類化合物，即含有nr3功能基團之化合 物’其中R為炫(基基團（Alkyl group)或芳香基團（Aryl group) ’特別是含有三曱基胺(trimethylamine)、二曱基胺 (dimethylamine)、一 曱基胺（monomethylamine)與氨 鲁 （ammonia)等功能基團之化合物的檢測。 本發明之胜肽序列係擇自由下列族群中：1322819 VI. Description of the invention: [Technical field to which the invention pertains] The present invention relates to a peptide compound, particularly to a peptide compound having high bonding sensitivity and bonding strength to an amine, which can be used for detecting an amine compound. . The present invention also relates to a method for detecting an amine compound using the above peptide compound. [Prior Art] In the mammalian olfactory system, the olfactory receptor protein is embedded in the cell membrane on the nasal mucosa. The olfactory receptor protein can react with the gas molecules to transmit the message to the olfactory region of the brain through a series of biochemical reactions. The type of taste. The olfactory receptor protein has different bonding positions for different gas molecules, depending on its amino acid sequence and the formed steric structure; the present invention uses this as a deduction to infer different peptide sequences to different gas molecules or compounds. Have different bonding capabilities. That is, with the sensor, different peptides are designed as the receiving membrane to achieve the purpose of detecting a specific compound. A related art in the prior art, such as US 6,218,507, utilizes a synthetic peptide to detect tridecylamine, except that its peptide sequence is completely different from the present invention. In many diseases, such as kidney disease, uremia, liver disease, stomach ulcers, etc., the amines in the body will be high, so it can be judged by measuring the content of amine compounds contained in the patient's breath. Such diseases. In addition, spoiled seafood such as fish and shellfish also release amine compounds, so the present invention can also be applied to the detection of seafood freshness. Similarly, the present invention is also applicable to the detection of the content of amine compounds in air or water, and then 1322819 to determine whether there is air pollution or water pollution. SUMMARY OF THE INVENTION The present invention provides a peptide compound which has high sensitivity to an amine compound and high bonding strength, and is suitable for detection of an amine compound. The invention also provides a method for detecting an amine compound by using the above peptide compound, comprising the steps of: contacting a substance to be tested with a sensing element, the sensing element being overcoated with the peptide compound; the substance to be tested and Sensing the signal obtained by the reaction of the component; and aligning the signal with a database to define the content of the amine compound in the substance to be tested. The invention also provides a device for detecting amines, comprising a sensing element overlying a peptide compound as described above, and a signal processing unit coupled to the sensing element for generating a signal. The peptide sequence of the present invention has high sensitivity and high bonding strength to an amine compound, and the peptide sequence or a derivative containing the peptide is used as a substance which reacts or detects with an amine compound having a low olfactory threshold value. It is used in the detection of sensors and as a tool for detecting amine compounds, it has the characteristics of improving sensitivity and applicability of low-concentration substances. The above and other objects, features, and advantages of the present invention will become more apparent and understood by the appended claims appended claims For the purpose of detecting amine compounds, the sequence structure of the protein 1322819 can be used to analyze the sequence characteristics of the peptide or the tertiary structure of the protein can be used to simulate the bonding position with the target molecule or a combination of amino acids according to the physical properties and chemical properties of the test substance. The peptide sequence is designed and synthesized manually; the obtained peptide compound is combined with a sensor or a wafer to output a signal, which can be used for an amine compound, that is, a compound containing an nr3 functional group, wherein R is dazzling ( Alkyl group or Aryl group 'especially contains functions such as trimethylamine, dimethylamine, monomethylamine and ammonia Detection of Compounds of Groups The peptide sequences of the present invention are selected from the following populations:
Asp-Pro-Asp-Gln-Arg-Asp (序列辨識號：1); Gly-Asp-Leu-Glu-Ser-Phe (序列辨識號：2);及 Glu-Tyr-Asp-Ser_Cys (序列辨識號：3)。 上述胜肽序列可視需要在其羧酸端及/或氨基端加上 一或複數個修飾基團，此修飾基團可為胺基酸或其它功能 基團’胺基酸如：胺基丙酸(alanine)、精胺酸(arginine)、 I 天門冬醯胺（asparagine)、天門冬胺暖(aspartic acid)、半胱 胺酸(cysteine)、麩胺酸酿胺（glutamine)、麵胺酸(glutamic acid)、甘胺酸（glycine)、組織胺酸（histidine)、異白胺酸 (isoleucine)、白胺酸(leucine)、離胺酸（lysine)、曱硫胺酸 (methionine)、苯胺基丙酸（phenylalanine)、脯胺酸 (proline)、絲胺酸（serine)、息寧胺酸(threonine)、色胺酸 (tryptophan)、路胺酸(tyrosine)、及綠胺酸(valine)等，功能 基團則可擇自含-COOH、-NH2、-CHO、-0H、或-SH之化 合物，較佳者為含-SH之化合物。 5 1322819 本發明之以胜肽化合物檢測胺類之方法，係利用前文 所述之胜肽化合物可與胺類化合物所產生鍵結作用的特 性，而達到檢測樣本内胺類化合物、其液相、或其揮發性 氣體之含量的目的。第1圖係本發明以胜肽化合物檢測胺 類之方法的流程圖’包括下列步驟：以一感測元件感測一 待測物質’此感測元件上覆被有前述之胜肽化合物；處理 所得之信號；以及將信號與一已建立的資料庫比對，以定 義待測物質中的胺類化合物含量。胺類化合物在此是指具 有NR3功能基團之化合物，其中R為烷基基團或芳香基 團，例如具三曱基胺、二甲基胺、一甲基胺、及氨基團之 化合物。上述方法包括：在一感測元件上，固定前文所述 之胜肽化合物’使此感測器與一樣本接觸，藉由偵測此胜 肽化合物與胺類的結合，以檢測該樣本所含之胺類化合 物。其中可作為感測器者如化學感測器、生物感測器或電 子鼻的接受、生物晶片，其感測元件之傳導器（transducer) 可為壓電石英晶體(piezoelectric quartz crystal)、表面聲波 (surface acoustic wave)、電化學（electrochemical)、光纖(fiber optic)、表面電聚共振（surface plasmon resonance)、金屬氧 化物半導體(metal oxide semiconductor)。其中壓電晶體生 物感測器是一項近年頗受重視的檢測工具之一，具有一壓 電石英晶體作為傳導器，其接受器是在壓電石英晶體上覆 被前述之胜肽化合物，當胜肽化合物與胺類分子反應時， 質量的改變便會影響壓電石英晶體的頻率，故反應時的強 度便可根據石英晶體的頻率改變來指示。而本發明之胜肽 序列對胺類化合物具有極佳的鍵結敏感度及鍵結強度’在 A ~ 類化&物上提供極佳的敏感度。本發明之方法中， 人、—之樣本可為氣體、液體、或固體，舉例來說，如空氣、 體呼氣、水、海產新鮮度、動物或人體的血液或尿液， 液或體液之揮發性氣體。 本發明檢測胺類之方法可應用的範圍相當廣泛，例如 ^1由栝,則人體呼氣的樣本中所含的胺類化合物含量而判 病八疾ΐ狀態，此類疾病的例子有：腎臟病、尿毒症、肝 产拷及胃潰€等症狀皆可藉由人體呼氣的胺類化合物的浪 =化而檢測；魚類或海產新鮮度檢測、空氣污染之檢測、 及水質檢測。 士發明之檢測胺類之裝置，包括一感測元件，其上覆 I元:文所述胜肽化合物，及一信號處理單元耦接於該感 ^ 用以產生一彳s號。上述感測元件可為前述應用各種 、化予形式傳導器之感測元件或生物晶片。 為了進_步闡明本發明之方法、特徵、和優點，以下 二子壓電晶體生物感測器）作為感測器，提出實施例 作砰細說明如下： 於辦發月人以嗅覺接友蛋白的立體結構為板模，利用電腦 _ Insight II模擬與胺類化合物作用的可能位置的胜肽 “再根據氨基酸之特性加以修飾此脞肽序列，透過以電 腦模擬設計龍減合物具有專—性、高錄度反應之胜 肽=列，將之作為電子鼻的接受膜，進而用於檢測具胺類 土團之化合物。電子鼻的傳導器（transducer)是採用 壓電石英晶體（piezoeiectric quartz crystal) ’ 再將設 计之胜肽覆被於石英晶體上，以電子鼻系統分析對胺類化 1322819 合物之反應。 【實施例】 製備例1 :胜肽之合成 本發明胜肽可以人工合成方式取得，如固相合成（solid phase synthesis) ' 液態胜肽合成(liquid peptide synthesis)、 酵素合成（enzymtic synthesis)、或DNA重組技術 (recombinant DNA technology)。本實施例依據固相合成， 以Wang resin為樹脂，F-moc為保護基，經由胜肽合成儀 (Apply Biosystems，432A Peptide Synthesizer，USA)合成。 製備例2 :修飾與覆被胜肽於壓電晶體金電極表面 設計胜肽序列時’利用硫可與金形成穩定的共價鍵， 故在胜肽的叛基端或氨基端加上含的胺基酸：半胱胺 酸，再依胜肽的溶解度以適當的有機溶劑稀釋，覆被在具 有金電極的壓電晶體表面上。胜肽之覆被方式是取24^^ 的/谷液覆蓋於壓電晶體之金電極上，反應4 &後，彳貞測頻 率下降值至15000〜20000 Hz左右即可，但有些分子性質較 特殊則依反應結果調整覆被量。 製備例3 :揮發性氣體之製備 分將試藥級三甲基胺、二甲基胺、一甲基胺、氨、丙明、 ^酸、丁酸、乙㈣酸、乙醇、及甲料化合物各別溶於 f發性有機減5 ml，置於⑽ml的密壯清瓶中，平衡 天待上部空間氣體達飽和蒸聽，由溶液的濃度及飽和 1322819 蒸氣壓值可換算得知上部空間氣體的濃度。依所需可利用 上部空間氣體再稀釋使用或直接抽取分析。 為了便於敘述，以下將本發明之胜肽序列予以編號如 下： 胜肽序列 1 (序列辨識號：1 ):Asp-Pro-Asp-Gln-Arg-Asp (SEQ ID NO: 1); Gly-Asp-Leu-Glu-Ser-Phe (SEQ ID NO: 2); and Glu-Tyr-Asp-Ser_Cys (SEQ ID NO: :3). The above peptide sequence may be optionally added with one or more modifying groups at its carboxylic acid end and/or amino terminus. The modifying group may be an amino acid or other functional group 'amino acid such as: aminopropionic acid. (alanine), arginine, I asparagine, aspartic acid, cysteine, glutamine, and face acid Glutamic acid), glycine, histidine, isoleucine, leucine, lysine, methionine, anilino Phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, etc. The functional group may be selected from compounds containing -COOH, -NH2, -CHO, -OH or -SH, preferably a compound containing -SH. 5 1322819 The method for detecting an amine by a peptide compound of the present invention is a method for determining an amine compound, a liquid phase thereof, and a salt compound which can be bonded to an amine compound as described above. Or the purpose of its volatile gas content. 1 is a flow chart of a method for detecting an amine by a peptide compound of the present invention, which comprises the steps of: sensing a substance to be tested by a sensing element; the sensing element is overcoated with the aforementioned peptide compound; The resulting signal; and aligning the signal with an established database to define the amount of amine compound in the substance to be tested. The amine compound herein refers to a compound having an NR3 functional group, wherein R is an alkyl group or an aromatic group such as a compound having a tridecylamine, a dimethylamine, a monomethylamine, and an amino group. The method comprises: immobilizing a peptide compound as described above on a sensing element to cause the sensor to be in contact with the same substrate, and detecting the binding of the peptide compound to the amine to detect the sample Amine compounds. The sensor can be used as a sensor, such as a chemical sensor, a biosensor or an electronic nose. The transducer of the sensing element can be a piezoelectric quartz crystal or a surface acoustic wave. (surface acoustic wave), electrochemistry, fiber optic, surface plasmon resonance, metal oxide semiconductor. Among them, the piezoelectric crystal biosensor is one of the most important detection tools in recent years. It has a piezoelectric quartz crystal as a conductor, and its receiver is coated with the above-mentioned peptide compound on the piezoelectric quartz crystal. When the peptide compound reacts with the amine molecule, the change in mass affects the frequency of the piezoelectric quartz crystal, so the intensity at the time of the reaction can be indicated by the change in the frequency of the quartz crystal. The peptide sequence of the present invention has excellent bonding sensitivity and bonding strength to amine compounds, and provides excellent sensitivity in A ~ classifying & In the method of the present invention, the sample of human, may be a gas, a liquid, or a solid, for example, such as air, body exhalation, water, seafood freshness, animal or human blood or urine, liquid or body fluid. Volatile gas. The method for detecting amines of the present invention can be applied to a wide range. For example, the content of the amine compound contained in the sample exhaled by the human body is judged to be eight diseases, and examples of such diseases are: kidney. Symptoms such as illness, uremia, liver production and stomach ulcers can all be detected by the wave of amines exhaled by the human body; fish or seafood freshness detection, air pollution detection, and water quality testing. The device for detecting amines, comprising a sensing element, is overcoated with a peptide compound, and a signal processing unit coupled to the sensor for generating a 彳s number. The sensing element described above can be a sensing element or biochip of the various applications described above. In order to clarify the method, features, and advantages of the present invention, the following two sub-piezoelectric crystal biosensors are used as sensors, and the embodiments are briefly described as follows: The three-dimensional structure is a plate model, and the peptide is simulated by the computer _ Insight II to simulate the possible position of the action of the amine compound. "The 脞 peptide sequence is modified according to the characteristics of the amino acid, and the dragon condensate is designed to be specialized by computer simulation. The peptide of high-reaction reaction = column, which is used as a receiving film for electronic nose, and is used to detect compounds with amine-like soil. The electron nose is made of piezoeiectric quartz crystal. The designed peptide was coated on a quartz crystal and analyzed by an electronic nose system for the reaction of the amined 1322819. [Examples] Preparation Example 1: Synthesis of the peptide The peptide of the present invention can be artificially synthesized. Obtained, such as solid phase synthesis 'liquid peptide synthesis, enzymtic synthesis, or DNA recombination technology (recomb) Inant DNA technology. This example was synthesized according to solid phase synthesis, using Wang resin as resin and F-moc as protecting group, and synthesized by a peptide synthesizer (Apply Biosystems, 432A Peptide Synthesizer, USA). Preparation 2: Modification and coating When the peptide is designed on the surface of the piezoelectric crystal gold electrode, the sulfur can form a stable covalent bond with gold, so the amino acid contained in the base or amino end of the peptide is added: cysteamine Acid, and the solubility of the peptide is diluted with a suitable organic solvent and coated on the surface of the piezoelectric crystal with a gold electrode. The method of coating the peptide is to take 24^^ of the valley solution to cover the gold of the piezoelectric crystal. On the electrode, after the reaction 4 &, the frequency drop value of the test can be about 15000~20000 Hz, but some molecular properties are more special, the coating amount is adjusted according to the reaction result. Preparation Example 3: Preparation of volatile gas will be The reagent grade trimethylamine, dimethylamine, monomethylamine, ammonia, propylamine, acid, butyric acid, ethyl (tetra) acid, ethanol, and nail compound are dissolved in f-organic organic minus 5 ml. Place in a dense bottle of (10) ml and balance the gas in the upper space. Saturated steaming, the concentration of the upper space gas can be converted from the concentration of the solution and the vapor pressure of the saturated 1322819. The upper space gas can be reused or directly extracted and analyzed as needed. For the convenience of description, the following will win the present invention. The peptide sequences are numbered as follows: peptide sequence 1 (sequence identification number: 1):
Asp-Pro-Asp-Gln-Arg-Asp ； 胜肽序列 2 (序列辨識號：2 )： ® Gly-Asp-Leu-Glu-Ser-Phe ;及 胜肽序列3 (序列辨識號：3) ·· Glu-Tyr-Asp-Ser-Cys。 實施例1 : 將胜肽序列1 (序列辨識號：1)覆被於電子鼻壓電晶 體，藉助電子鼻分析系統（Smart Biotechnology Co.，Ltd.， Taipei，Taiwan)，對三曱基胺、二曱基胺、一曱基胺、及氨 等四種胺類，及其它上述之化合物的上部空間揮發氣體個 _ 別進行反應，用於電子鼻分析之氣體約為5 mg/1左右，比 較反應之專一性及靈敏度，由其中篩選出對上述胺類化合 物反應最佳的胜肽序列。 此三條胜肽覆被於電子鼻壓電晶體上的量，根據 Sauerbrey equation (Sauerbrey，1959) ’ 壓電晶體頻率下降值 與其上質量的改變成正比，故以胜肽覆被後之頻率下降值 (Hz)表示覆被量大小，本實施例中，胜肽序列1 (序列辨識 號：1)之覆被量為2194Hz ’其對三甲基胺、二曱基胺、 一曱基胺、氧、丙綱、曱酸、丁酸、乙稀醋酸、乙醇、及 9 1322819 曱醛等有機揮發性氣體反應之頻率下降值如第2圖所示。 由第2圖中可以看出胜肽序列1 (序列辨識號：1 )對三曱 基胺、二曱基胺、一曱基胺、及氨的反應最明顯、專一性 最佳，而對其他的有機揮發性氣體反應很差，顯示胜肽序 列1 (序列辨識號：1 )極適合用來檢測胺類化合物。 實施例2 : 利用胜肽序列2 (序列辨識號：2)，以實施例1所述 之方法測量其對各類化合物的反應。本實施例中，胜肽序 列2 (序列辨識號：2)之覆被量為283 Hz，其對各種有機 揮發性氣體反應之頻率下降值如第3圖所示。由第3圖中 可以看.出胜肽序列2 (序列辨識號：2)對三曱基胺、二曱 基胺、一甲基胺、及氨的反應最明顯、專一性最佳，而對 其他的有機揮發性氣體反應很差，顯示胜肽序列2 (序列 辨識號：2)極適合用來檢測胺類化合物。 實施例3 : 利用胜肽序列3 (序列辨識號：3)，以實施例1所述 之方法測量其對各類化合物的反應。本實施例中，胜肽序 列3 (序列辨識號：3)之覆被量為620 Hz，其對各種有機 揮發性氣體反應之頻率下降值如第4圖所示。由第4圖中 可以看出胜肽序列3 (序列辨識號：3)對三曱基胺、二曱 基胺、一曱基胺、及氨的反應最明顯、專一性最佳，而對 其他的有機揮發性氣體反應很差，顯示胜肽序列3 (序列 辨識號：3)極適合用來檢測胺類化合物。 1322819 雖然本發明已以較佳實施例揭露如上，然其並非用以 限定本發明，任何熟習此技藝者，在不脫離本發明之精 神和範圍内，當可作些許之更動與潤飾，因此本發明之 保護範圍當視後附之申請專利範圍所界定者為準。 1322819 【圖式簡單說明】 第1圖：本發明以胜肽化合物檢測胺類之方法的流程 圖； - 第2圖：胜肽序列1 (序列辨識號：1)對各種有機揮 發性氣體反應之頻率下降值比較圖； 第3圖：胜肽序列2 (序列辨識號：2)對各種有機揮 發性氣體反應之頻率下降值比較圖； 第4圖：胜肽序列3 (序列辨識號：3)對各種有機揮 發性氣體反應之頻率下降值比較圖。 籲 【主要元件符號說明】 無。 12 1322819 序列表 <110>財團法人工業技術研究院 <120>胜肽化合物及使用其檢測胺類之方法 <140> 90131930 <141> 90 年 12 月 21 日 <160〉 3 <210> 1 <211> 6 <212>胺基酸 <213>人工序列 <220> <221>用於檢測胺類化合物之胜肽化合物 <400> 1Asp-Pro-Asp-Gln-Arg-Asp ; peptide sequence 2 (SEQ ID NO: 2): ® Gly-Asp-Leu-Glu-Ser-Phe; and peptide sequence 3 (SEQ ID NO: 3) · Glu-Tyr-Asp-Ser-Cys. Example 1: The peptide sequence 1 (sequence number: 1) was coated on an electronic nose piezoelectric crystal, and the trimethylamine was used, by means of an electronic nose analysis system (Smart Biotechnology Co., Ltd., Taipei, Taiwan). Four kinds of amines such as dimercaptoamine, monodecylamine, and ammonia, and other upper space volatile gases of the above compounds are reacted, and the gas used for electronic nose analysis is about 5 mg/1. The specificity and sensitivity of the reaction, from which the peptide sequence optimal for the above amine compounds is selected. The amount of the three peptides coated on the electronic nose piezoelectric crystal, according to the Sauerbrey equation (Sauerbrey, 1959) 'the piezoelectric crystal frequency drop value is directly proportional to the change in the mass thereof, so the frequency decrease after the peptide is covered (Hz) indicates the size of the coating. In the present example, the amount of the peptide sequence 1 (sequence number: 1) is 2194 Hz 'the pair of trimethylamine, didecylamine, monodecylamine, oxygen The frequency decrease of the reaction of organic volatile gases such as propyl, citric, butyric acid, ethyl acetate, ethanol, and 9 1322819 furfural is shown in Fig. 2. It can be seen from Fig. 2 that the peptide sequence 1 (sequence number: 1) has the most obvious and specific reaction to tridecylamine, didecylamine, monodecylamine and ammonia, while other The organic volatile gas reacted poorly, indicating that peptide sequence 1 (SEQ ID NO: 1) is highly suitable for the detection of amine compounds. Example 2: Using the peptide sequence 2 (SEQ ID NO: 2), its reaction to various compounds was measured by the method described in Example 1. In the present example, the sample size of the peptide sequence 2 (sequence number: 2) was 283 Hz, and the frequency decrease value of the reaction with respect to various organic volatile gases is shown in Fig. 3. As can be seen from Figure 3, the peptide sequence 2 (SEQ ID NO: 2) has the most obvious and specific response to tridecylamine, didecylamine, monomethylamine, and ammonia, but Other organic volatile gases react poorly, indicating that peptide sequence 2 (SEQ ID NO: 2) is highly suitable for the detection of amines. Example 3: The reaction of various compounds was measured by the method described in Example 1 using the peptide sequence 3 (SEQ ID NO: 3). In the present example, the sample size of the peptide sequence 3 (sequence number: 3) was 620 Hz, and the frequency decrease value of the reaction with respect to various organic volatile gases is shown in Fig. 4. It can be seen from Fig. 4 that the peptide sequence 3 (SEQ ID NO: 3) has the most obvious and specific reaction to trimethylamine, didecylamine, monodecylamine, and ammonia, while other The organic volatile gas reacted poorly, indicating that peptide sequence 3 (SEQ ID NO: 3) is highly suitable for the detection of amine compounds. The present invention has been disclosed in the above preferred embodiments, and is not intended to limit the invention, and it is to be understood that those skilled in the art can make some modifications and refinements without departing from the spirit and scope of the invention. The scope of the invention is defined by the scope of the appended claims. 1322819 BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a flow chart showing the method for detecting amines by peptide compounds in the present invention; - Fig. 2: peptide sequence 1 (sequence number: 1) is reacted with various organic volatile gases Comparison of frequency decrease values; Figure 3: Comparison of frequency decrease values of peptide sequence 2 (sequence identification number: 2) for various organic volatile gases; Figure 4: peptide sequence 3 (sequence identification number: 3) A comparison of frequency drop values for various organic volatile gases.吁 [Main component symbol description] None. 12 1322819 Sequence Listing <110> Institute of Industrial Technology <120> peptide compound and method for detecting amines thereof <140> 90131930 <141> December 21, <160>;210> 1 <211> 6 <212> Amino acid <213> Artificial sequence <220><221> peptide compound for detecting amine compound <400>
Asp Pro Asp Gin Arg Asp 1 5 <210> 2 <211> 6 1322819 <212>胺基酸 <213>人工序列 <220> <221>用於檢測胺類化合物之胜肽化合物 <400> 2Asp Pro Asp Gin Arg Asp 1 5 <210> 2 <211> 6 1322819 <212> Amino acid <213>Artificial sequence <220><221><400> 2
Gly Asp Leu Glu Ser Phe <210> 3 <211> 5 <212>胺基酸 <213>人工序列 <220> <221>用於檢測胺類化合物之胜肽化合物 <400> 3Gly Asp Leu Glu Ser Phe <210> 3 <211> 5 <212> Amino acid <213> Artificial sequence <220><221> peptide compound for detecting amine compound <400>; 3
Glu-Tyr-Asp-Ser-CysGlu-Tyr-Asp-Ser-Cys