1304338 日期：97年9月24日 第92113267號專利說明書修正本 七、指定代表圖： ί:ίίί定代表圖為:第（1 )圖。 I;)本代衣圖之元件符號簡單說明： ^ 才每明種破壞冠狀病毒致病性之活性劑，氫氧基 辛烷酸之化學式。 八 、本案若有化學式時，請揭示最能顯示發明特徵的化學式 九、發明說明： :【發明所屬之技術領域】 本發明係一種特殊的活性劑，具有破壞冠狀病毒外膜之致 病性，使冠狀病毒之外膜喪失對細胞吸附、侵入與感染之能 力。可應用在防護功用相關用品上，如口罩、防護衣二 相關空氣濾淨之濾材等。 及其他 13043第 1 92113267痛專利說明書修正本 日期：97年9月24日 I先前技術】 92年3月起迅速延燒·的嚴重急性呼吸道症候群 Acirte Respiratory Syndrome，SARS)震撼全球，並考驗著政 府、醫療體系舆醫藥界的應變能力，面對.一個新興致命冠狀病 毒的，行，主要工作有以下四個方向疫情控制?發展快速| 確的篩檢方法、開發抗冠狀病毒藥物以及疫苗的研發預防再流 f，醫學進步至今，疫情發生開始數月内即可認定引起之禍$ 是冠狀冠狀病毒（Coronavirus).，並將冠狀冠狀病毒基因解 碼，研發，出免疫螢光檢測試劑與酵素免疫分析法等篩檢方法， 但在開發抗冠狀病毒藥物以及疫苗的研發預防方面，預計則還 有半年到一年的時間，在這之前，最緊要的工作就是做好防^ φ 工作以疫情控制，降低疫情所帶來的經濟衝擊。 ^ 冠狀冠狀病毒是引起嚴重急性呼吸道症候群之病源，目前 的研究指.出，冠狀冠狀病毒主要藉由在空氣中懸浮的口涑微粒 ，播，造成感染，因此，本發明提出一種特殊的活性劑，具破 壞，狀冠狀病毒之對細胞吸附、侵入與感染之能力。可應用'在 防濩功，相關用品上，如口罩、防護衣以及其他相.關空氣濾淨 之濾·材等。 〜 、，世界衛生組織（World Health Organization，〇)與 美國疾病管制局（Centers for Disease Control and Prevention，、，CDC)所推薦防護嚴重急性呼吸道症候群之N95 φ 罩原疋美國才日疋防教肺結核病菌的口罩，可以有效虔么士 ，桿菌（tuberculosis，ΤΒ，直徑約為〇· 3〜〇· 6微米，^二 微米）防止空氣感染。所謂Ν95指的是美國職業安全衛生研究1304338 Date: September 24, 1997 Revision No. 92113267 Patent Specification VII. Designation of representative drawings: ί: ίίί The representative figure is: (1). I;) A brief description of the symbol of the clothing of the present generation: ^ The chemical formula of the hydroxyoctanoic acid, the active agent that destroys the pathogenicity of the coronavirus. 8. If there is a chemical formula in this case, please disclose the chemical formula which best shows the characteristics of the invention. 9. Description of the invention: [Technical field to which the invention belongs] The present invention is a special active agent which has the pathogenicity of destroying the outer membrane of the coronavirus. The outer membrane of the coronavirus loses its ability to adsorb, invade and infect cells. It can be applied to protective functional related products, such as masks, protective clothing and related air filter media. And other 13043 1st 92113267 pain patent specification revision date: September 24, 1997 I prior art] The severe acute respiratory syndrome (Acirte Respiratory Syndrome, SARS) that has been rapidly prolonged since March 1992 shocked the world and tested the government, The medical system and the resilience of the medical profession, facing the emergence of a new deadly coronavirus, the main work has the following four directions of epidemic control? Rapid development | Definitive screening methods, development of anti-coronavirus drugs and vaccine development and prevention Re-flow f, medical progress so far, within a few months of the onset of the epidemic can be identified as causing the cause of coronary coronavirus (Coronavirus), and the coronary coronavirus gene decoding, research and development, immunofluorescence detection reagent and enzyme immunoassay Screening methods such as law, but in the development of anti-coronavirus drugs and vaccine research and development prevention, it is expected to have half a year to a year, before this, the most important task is to do a good job of anti- φ work to control the epidemic, Reduce the economic impact of the epidemic. ^ Coronary coronavirus is the cause of severe acute respiratory syndrome. The current study indicates that coronary coronavirus is mainly caused by oral sputum particles suspended in the air, causing infection. Therefore, the present invention proposes a special active agent. , a damaging, coronavirus-like ability to adsorb, invade, and infect cells. It can be applied to 'in the anti-mite work, related products, such as masks, protective clothing and other related filters. ~,, World Health Organization (〇) and the US Centers for Disease Control and Prevention (CDC) recommended to protect against severe acute respiratory syndrome N95 φ hood 疋 疋 疋 疋 疋 疋 疋 肺 肺The mask of the pathogen can effectively prevent cockroaches, bacilli (tuberculosis, 直径, diameter about 〇·3~〇·6 microns, ^2 micron) to prevent airborne infection. The so-called Ν95 refers to the American occupational safety and health research.
所（National lnstitute f0r 0ccupatlonaLM NIOSH)制定的認證標準，N代表非油性微粒、阳為過濾係數，’ 根據美國國家職業安全衛生研究院年6月8日公佈之 塵類呼吸防護新標準42CFR84 ; μ 6 130411脚撕麵觀本 曰期：97年9月24日 N系列：防護非油性微粒、無時限 R系列··防護非油.性微粒及油性微粒、時限八小時 P系列：防護非油性微粒及油性微粒、無時限 每系列又劃分出了 3種過濾等級：95%、99%、99. 97% (即簡稱 爲95，99，100)，檢驗標準以〇· 3微米之氯化鈉（Nac])胃微 粒或是DOP (dioctyl phthalate)油霧的微粒測試。一般來 說，細菌的大小在1-0.1微米、病毒的大小則在[奈米， 至於為何美國職業安全衛生研究所之認證之微粒大小是以〇 3 微米為基準呢？可由其這與過濾機制來進行瞭有關，以過潰、 理論而言，主要有.以下幾種過濾微粒的方法： … 1·引力吸引（ Gravitati〇nl :Settling)4|^_i, (；[nertial(National lnstitute f0r 0ccupatlonaLM NIOSH) certification standards, N for non-oil particles, yang for the filter coefficient, 'according to the National Institute of Occupational Safety and Health, June 8th, the new standard for dust respiratory protection 42CFR84; μ 6 130411 foot tearing face view this period: September 24, 1997 N series: protective non-oil particles, no time limit R series · · non-oil protective particles and oil particles, time limit eight hours P series: protective non-oil particles and Oily particles, no time limit Each series is divided into three filter grades: 95%, 99%, 99.97% (abbreviated as 95, 99, 100), the test standard is 〇 · 3 micron sodium chloride (Nac ]) Particle testing of gastric particles or DOP (dioctyl phthalate) oil mist. In general, the size of the bacteria is between 1 and 0.1 microns, and the size of the virus is in [nano. As for why the size of the microparticles certified by the Occupational Safety and Health Research Institute is based on 〇 3 microns? This can be related to the filtering mechanism. In terms of over-cracking, in theory, there are mainly the following methods for filtering particles: ... 1 Gravitational attraction (Gravitati〇nl: Settling) 4|^_i, (;[ Nertial
Impaction)與攔截（Direci: Interception):物理性過濾機 制，主要對大於0· 3微米之微粒有效。 似 2·布朗運動（Brownian Diffusion):小於〇· 1微米之微粒會 因為空氣分子之布朗運動而撞在濾層纖雉上。 ^靜電吸引（ElectrotaticK·渡層纖維經過特殊極化處理變 駐極體（electret) ’適合捕捉中型及小型的微粒。 =國專利4,536，棚、4,807,619、5,3〇7,796 及 8〇 小微之機制_ 細=粒越，過渡的，是似是而4忒’。而一[' (Photocataly!^ ^ ^ ^ ^ ^ ^ 是利用奈米等級大小之二氧錢 1304规 ί267號專利說明書修正本 日期：97年9月24日 空ΐ氧二匕，受5外光（ultravi—)照 的空穴句電子與毁能參與光觸媒反應，形成 團殘，原子團與外界細菌等有機化合物 ^伽之必要條件之—紫外光纽類 五二2紫外光不夠充足之處便形成過渡死肖，^不要提直 X 2=^〇十奈米尊級二氧化欽奈米顆粒之造價有多高！ - if述現有之過據機制與赌，考慮著眼點以針對 死角又t粒=及是否為油性兩點’而光觸職材有過濾 又^面對目前s廳風暴所帶來的口罩荒，本發明 胞吸^1殊？?Ϊ病毒之活性劑，可破壞冠狀病毒外膜對細 如寸、&人域‘之能力’可應用在防護魏相_品上， 如及其他蝴空㈣淨之餘等，以提供更專 【發明内容】 根據目前所發表之報告與冠狀病毒基因圖譜可分析得 t冠狀病毒如第二圖所示是一直徑大小.約，4〇奈米之丽 ;丙毋周圍有類似皇泡之突觸套膜（enve:l〇敗），套膜之主要 ，成分之-_蛋白(· 1 i PQprcteins)，脂蛋自之成分有脂質、 〜脂質（phospholipid) 50-60%、膽固醇（ch〇iester〇i) (Triglyceride5 肪），一般冠狀病毒粒外膜之蛋白質相對於被攻擊細胞表面受 體間之親和性，決定冠狀病毒粒對宿主細胞之攻擊性，與冠狀 病毒對^纟:胞之吸附、侵入與感染有關，依此原理，本發明提出 以8-氫氧基辛烷酸作為抗冠狀病毒之活性劑，藉由&氫氧基 辛烧酸破壞；^狀病毒外膜中蛋白質的三度空間構形 (three-dimensional conf〇rmai；i〇n)，使冠狀病毒粒失去吸附 和侵入宿主細胞的能力，進而喪失感染性。 1304338 曰期：97年9月24日 第92113267號專利說明書修正本 則為酸基[-C00H]的直鏈气1^^〇Η；1>而另一端 [HOCCHOvCOOE] 〇 硬土铵虱化合物，化學式為 氨氧性劑. 罩、防護衣、;人廣播,ί作為4寸知慮層，應用在防護口 ΐΐί 11個，铜狀絲吸_可;x是帶正電靜電 呈現帶妓職、材_純基_使濾材 ί實施方式】 本發明係一種特殊的活性劑，具有破壞冠狀病毒外膜之 致病=的能力，該活性劑主要成分為8一氫氧基辛烷酸。、 以下說明8-氫氧基辛烷酸合成方式、： 的合成方法 業上可獲得的正一溴基烷酸[Br(CH2>C00H]l當量加入5 的氫氧化鋰[Li〇H]，溶在5毫升/1當量的水和5毫升八 田1的1，4一二氧陸圜（1，4-Dioxane)混合液中，加熱迴流 十】、bm，待反應溫度降為室溫後加入2N gci進行酸化至 pH-2〜3 ’利用乙醚萃取並分別用飽和碳酸氫納水溶液及飽和 食鹽水清洗乙醚層，再用無水硫酸鎮乾燥、管柱層析純化即 可得正-氫氧基烷酸化合物[H0(CH2>C00H]。 本發明所提之一種破壞冠狀病毒外膜致病性之活性劑， η亥活性劑主要组成為8_氫氧基辛烧酸，可以渡材浸泡之，製 1304338 ^ 第92113267藏專利說明書修正本 3特殊濾層’應用在防護口罩 濾清系統等過濾系統中。 邊衣 曰期 :97年9月24臼 、冷氣機以及空氣 靜電之駐極體，細:②：層 讀ΐί此之較佳實施例係已在此充分地詳鈿描述， 是吾Uir之人士能_用本發明。然應當了解的 不合北齙rl"十對所說明之實施例進行改變、修改、替換而 V月本I明於隨附申請專利範圍所界定之精神與範嘴、。 【圖式簡單說明】 二:t氫氧基辛燒酸之化學式 θ魅狀病毒之電子顯微鏡照片 無 【主要元件符號說明】 10Impaction and Interception: A physical filtration mechanism that is effective for particles larger than 0.3 microns. Brownian Diffusion: Particles smaller than 〇·1 μm collide with the filter fiber due to Brownian motion of air molecules. ^Electrostatic attraction (ElectrotaticK·crossing fiber is specially polarized to elect electret) (suitable for capturing medium and small particles) = National patent 4,536, shed, 4,807,619, 5,3〇7,796 and 8〇小微之Mechanism _ fine = grain, the transition, is like and 4 忒 '. And one [' (Photocataly! ^ ^ ^ ^ ^ ^ ^ is the use of nano-scale dioxy money 1304 regulations 267 patent specification amendments Date: September 24, 1997, the empty oxygen sputum, the electrons and ruin of the 5th external light (ultravi-) are involved in the photocatalytic reaction, forming a group residue, the atomic group and the external bacteria and other organic compounds. Conditions - UV light New Zealand 520 2 UV light is not enough to form a transitional death, ^ Do not straighten X 2 = ^ 〇 ten nanometer esteem two-dimensional chlorinated granules of the cost of how high! - if The existing mechanism and gambling, considering the point of view to target the dead angle and t grain = and whether it is oily two points 'and the light touches the material has a filter and face the current mask storm caused by the storm, the present invention The cytotoxicity of the prion is the active agent of the prion, which can destroy the outer membrane of the coronavirus. , && human domain 'ability' can be applied to the protection of Wei Xiang _ goods, such as and other emptiness (four) net surplus to provide more specialized [invention content] According to the current published report and coronavirus gene map can be Analysis of the t-coronavirus as shown in the second figure is a diameter of about. About 4 〇Nin Li; Bing 毋 around there is a synaptic membrane similar to the Emperor (enve: l 〇), the main film, Ingredients - protein (· 1 i PQprcteins), fat egg from its constituents are lipids, ~lipidid 50-60%, cholesterol (ch〇iester〇i) (Triglyceride5 fat), general coronavirus outer membrane The affinity of the protein relative to the receptor on the surface of the attacked cell determines the aggression of the coronavirus particle to the host cell, and is related to the adsorption, invasion and infection of the coronavirus to the cell, and according to this principle, the present invention proposes 8 -Hydroxyoctanoic acid as an active agent against coronavirus, destroyed by & hydroxyoctanoic acid; the three-dimensional configuration of the protein in the outer membrane of the virus (three-dimensional conf〇rmai; i〇 n), the coronary granules lose their adsorption and invade the host The ability to lose the infectivity. 1304338 曰期: The revised specification of the 9211267 patent on September 24, 1997 is the linear gas of the acid group [-C00H]; 1> and the other end [HOCCHOvCOOE] 〇 hard earth ammonium bismuth compound, chemical formula is ammonia oxidizing agent. Cover, protective clothing, human broadcast, ί as a 4-inch layer of concern, applied in protective mouth ΐΐ 11 11, copper wire _ _; x is positive The invention relates to a special active agent, which has the ability to destroy the pathogenicity of the outer membrane of the coronavirus, and the main component of the active agent is 8-hydrogen oxygen. Keic acid. The following is a description of the synthesis method of 8-hydroxyoctanoic acid: a synthetic method of n-monobromoalkanoic acid [Br(CH2>C00H]l equivalent of 5 lithium hydroxide [Li〇H], Dissolved in a mixture of 5 ml / 1 equivalent of water and 5 ml of Octa 1 in 1,4-Dioxane, heated to reflux, and bm, after the reaction temperature is lowered to room temperature, 2N gci was acidified to pH-2~3', extracted with diethyl ether and washed with saturated aqueous sodium hydrogencarbonate solution and saturated brine, respectively, and then dried over anhydrous sulfuric acid and purified by column chromatography to obtain n-hydroxyl Alkanoic acid compound [H0(CH2>C00H]. The present invention provides an active agent for destroying the pathogenicity of the outer membrane of a coronavirus, and the main component of the active agent is 8-hydroxyoctyl succinic acid, which can be immersed in the material. , system 1304338 ^ No. 92113267 Tibetan patent specification revision 3 special filter layer 'applied in filtration system such as protective mask filtration system. Sidewear period: September 24, 1997, air conditioner and air static electret, Fine: 2: Layer reading ΐ The preferred embodiment of this is described in full detail here, is my Uir The person can use the present invention. However, it should be understood that the embodiment of the ten 所 rl" is changed, modified, and replaced, and the V-monthly I is clearly defined in the spirit and van. [Simple description of the diagram] 2: Electron micrograph of the chemical formula θ sympathy virus of t-hydrogen octanoic acid is not available [Main component symbol description] 10