TWI250280B - Health and safety DIY (Beefan reagent) kit-the hydroquinone in whiten cosmetics simple and rapid test method - Google Patents

Abstract

This invention is in view of the market cosmetics use beautiful white ingredient hydroquinone illegally. Instead of being operated in the laboratory only, the method has been modulated into test reagent (Beefan reagent). Due to operating easily and reacting quickly, the test reagent can be used by any consumers wherever at home or any market spots.

Description

1250280 玖'Invention Description: [Technical Field of the Invention] The principle of the "simple rapid test method for hydroquinone in whitening cosmetics" is based on the use of qualitative methods in potassium iodate (KI〇3) under acidic conditions. In the fading reaction of oxidized azure (azure B), the addition of trace amounts of hydroquinone has a significant catalytic effect. According to this principle, it is formulated into a simple reagent-biphenol reagent, which is directly added to the whitening cosmetic. It is observed whether the color of the solution changes to a yellowish brown after the drip of the cosmetic. The color change reaction can quickly detect whether the whitening component is added to the benzene in the cosmetic. Diphenol. The sensitivity and specificity of the detection of hydroquinone by the behenol reagent are good, as long as the cosmetic contains 0.05% or more of the benzoquinone. [Prior Art] Basis: The relevant testing methods of the Drug and Food Inspection Bureau of the Department of Health and the standard operating procedures for the inspection methods of the laboratory of the Taipei Municipal Government Health Bureau. Operation method: 1. Pre-treatment of the sample (1) Take about 200 to 400 mg of the sample in a 25 mL dosing bottle to accurately record the sample volume. (2) Add about 20 mL of reagent water. (3) Set the oscillation in the ultrasonic oscillator for 30 minutes. (4) Make up to 25 mL. ^ (5) The test solution is filtered with a nitrocellulose filter. Qualitative analysis by layer chromatography, II. Qualitative and quantitative analysis by liquid chromatography with liquid performance [Invention] The invention is aimed at adding cosmetics for hydroquinone, which will be operated in the laboratory. After the innovative method is prepared into a simple and rapid detection reagent, it can be detected by the general public at home or at any store. [Embodiment] 1. Preparation of Biyi reagent (1) The reagent azure B is formulated into an aqueous solution of 50~150 ppm concentration; (2) Dissolving potassium iodate (KI〇3) in hot water, and then Formulated into an aqueous solution of 1.0% (w/w) concentration; (2) mixing 50~150 ppm aqueous solution of azure with 1.0% aqueous potassium sulfate solution in an equal amount of 1: l (v/v); 6 125 〇28〇(4) The concentration of azure solution becomes 25~75 ppm, and the concentration of potassium iodate solution becomes Q. 5 0/〇(v/v); (5) It is adjusted to 0° with pH of phosphoric acid (HsPO4) solution; $ 2 is the bisphenol reagent, which is then dispensed into a suitable container for easy and quick testing of cosmetics. , simple and rapid test reagents, the steps of the reagents · · (a) as shown in the first diagram of the flow chart; remove the whitening cosmetics, the above-mentioned already prepared behenol reagent directly drops a few drops to the whitening cosmetics sample; (1) In the case of hydroquinone-containing, it is positive: the cosmetic is exposed to the phenolic reagent, and the color of the solution at the drip point gradually turns yellowish brown; (a) the one that does not contain hydroquinone is negative. · Cosmetics are exposed The tourmaline reagent observed that the color of the solution at the drip was unchanged and remained blue. Please refer to the attached photo; Photo 1: The original color of the cosmetic sample; Photo 2: Drop the test fiber for the cosmetic sample; Photo 3: The color of the cosmetic sample is changed from blue to yellowish brown. The color change reaction indicates that the cosmetic sample may contain n, the film 4: the original color of the cosmetic sample; the photo 5: for the cosmetic sample, the addition of the age, the detection of the six. The cosmetic sample contact with the test face color and money, Indicates that the cosmetic sample does not contain benzophenone. [Simplified illustration of the diagram] The first picture: the flow chart of the detection steps. [Attachment] Lower 1250280 Photo 2: For the cosmetic sample (positive sample containing hydroquinone), add the test solution of the phenolic reagent. Photo 3: The color of the cosmetic sample is changed from blue to yellowish brown, and its color change reaction Indicates that the cosmetic sample may contain hydroquinone photo four: the color of the original color of the cosmetic sample. Photo 5: For the cosmetic sample (negative sample without hydroquinone), add the phenol reagent test photo 6: Cosmetics inspection There is no change in the color of the body contact bisphenol reagent, indicating that the cosmetic sample does not contain hydroquinone. 8

Claims (1)

1250280 Fan, f animal patent Fan Park: ι·= whitening cosmetics in the qualitative test method for the rapid detection of benzene II, the steps of the US speed test reagent (Wei reagent) directly drop a few drops to the batch to be inspected (this two, Miscellaneous reaction ····································································································· If the color of the agent is changed, the color of the reagent is unchanged, and it is still blue. For example, please refer to the qualitative method of the first paragraph of the patent range, in which the whitening cosmetic is a facial cleanser. • If the standard of the patent scope of Shen Qing is the first item The method, wherein the whitening cosmetic is a lotion. The method of applying the patent scope of the third item, wherein the whitening cosmetic is an emulsion. 5. The method of applying the patent scope of the third item, wherein the whitening cosmetic is a face cream. The qualitative method of the i-th item of the Xiang range, in which the whitening cosmetics are masks. 7.-, used in the «Quick test of whitening cosmetics for the test of benzodiazepines (Bai Lai, the preparation steps are as follows: (1) will try Azure B is formulated into a 50~15〇 concentration aqueous solution; (=) dissolved in hot water __(pass), reconstituted & L 0 % concentration aqueous solution; (3) 50~150 ppm azure The aqueous solution is mixed with 丨·〇% potassium iodate aqueous solution in an equivalent amount of 丨:1 (v/v); (4) that the concentration of the azure solution becomes 25 to 75 ppm, and the concentration of the potassium acid solution becomes 〇·5 % (v/v) (5) Adjust the phosphoric acid (HsPO4) solution to 〇<pH^2, which is the phenol reagent, and then dispense the reagent into a suitable container for easy and quick test of cosmetics. Apply for the reagent of item 7 of the patent scope, in which the reagent azure is formulated into a solution of 5 〇ppm concentration. 9. If the reagent of the seventh paragraph of the patent application is applied, the reagent azure is formulated to be 75, and the concentration is Solution 10. If the reagent of the scope of patent application No. 7 is used, the reagent azure is formulated into a solution of 1〇〇ppm concentration. 11. If the reagent of the patent scope is applied, the reagent is prepared by azurol. A solution of 15 〇 ppm concentration.