TWI244376B - Method of emulsifying phytosterol by natural saponins, emulsion prepared thereby and water dispersible phytosterol powder product - Google Patents

Abstract

Method of emulsifying phytosterol by natural saponins is disclosed including mixing an oil phase having phytosterol dissolved therein and an aqueous phase having a natural hydrophilic surfactant (saponins) therein to obtain an emulsion of phytosterol. A water dispersible phytosterol powder product can be formed by drying the emulsion, which is able to be dispersed in an aqueous solution or beverage. The phytosterol emulsion, the phytosterol powder product and the aqueous dispersion thereof may be used in the cosmetic products and food stuffs, thereby enhancing the value of the phytosterol.

Description

I244376 发明 Description of the invention: Technical field to which the invention belongs The present invention relates to a method for emulsifying plant sterols, and more particularly to a method for emulsifying plant sterols with natural surfactants. Prior technology Phytosterol, full name Phytosterol, contains three major classes of C27 4-desmethyl, C28 4-desmethyl and C29 4-demethyl phytosterol. For example: desmosterol, campesterol, dihydrobrassicasterol, sitosterol, stigmasterol1, etc. Phytol and cholesterol have similar molecular structures, so they interfere with the absorption of cholesterol in the human intestine, thereby preventing cholesterol from being taken too high 'to reduce the incidence of cardiovascular disease. In addition, exophytol has important physiological activities in the literature to enhance human immunity and reduce the incidence of cancer. Last year, many of the top ten causes of death among Chinese people were related to high blood cholesterol, including cerebrovascular disease, heart disease, high blood pressure, etc. These diseases have a tendency to increase year by year. How to lower blood cholesterol has become an important medical issue. The existing cholesterol-lowering drugs on the market are mainly "r Statins", which are suitable for patients with primary hypercholesterolemia and can effectively reduce low-density lipoprotein cholesterol ', can also slightly reduce triglycerides, and increase high-density lipoprotein cholesterol. Statins may cause serious side effects such as rhabdomyolysis. For example, Baycoi 1244376 (cerivastatin), which was approved for marketing in the United States in 1997, has caused more than 100 deaths worldwide due to severe side effects of the drug. Bayer also faces global Huge compensation lawsuit. The best-selling cholesterol-lowering Statins-type prescription drugs and phizers are strict Lipitor ’depending on the prescription, the total cholesterol lowering effect is about 29% to 45%. The effect of phytosterols on lowering blood cholesterol has been confirmed by dozens of medical literatures. Generally speaking, ingesting i.4 grams of phytosterols daily for one month can reduce the total cholesterol concentration by about 14/0. Although the effects of plant sterols are slightly worse than those of Statins, plant sterols have the advantages of natural, no side effects, and lower production costs. Therefore, the US Food and Drug Administration published a document (T00-40) on September 5, 2000, which acknowledged that daily intake of more than 3 grams of plant sterols can significantly reduce the cholesterol concentration of LDL, and thus effectively reduce cardiovascular disease (such as coronary Arterial heart disease). The effect of plant sterols on lowering cholesterol in blood was studied about 50 years ago, but it was not until 1995 that the company ○ in Finland introduced the world's first product made of plant sterols. The biggest reason is that the purified plant solids are difficult to dissolve in water or oil and most solvents, which makes the design of plant sterols difficult. On the other hand, the absorption rate of directly purified plant sterols in the human body is less than 5%, and the absorption rate of cholesterol in the human body is more than 50 /. In order to formulate products and increase the availability of phytosterols, phytosterols must be converted to a form suitable for food addition after some processing or formulation. For example, the Umlever company in Finland and later introduced the production of mouth, esterified plant sterols with unsaturated fatty acids. The vinegarized plant sterols have good oil solubility, and then add them to cream or 1244376 oil. Products. Phytosterols in esterified form are the first derivatives

For example, the most common derivative form (W0 9956558, 1999; US 20002/0160990, 2002; US 6,492,538, 2002), but the problem of oil-soluble formulas is greatly increased. It is inevitable that while ingesting phytol, it will inevitably eat a lot of fat on the same day, and fat may cause other problems such as obesity. In terms of emulsifying plant sterols, it is mainly to use plant emulsifiers to coat plant sterols to form microlipids in aqueous solutions, which will greatly improve the effect of plant sterols. In the aspect of miniaturization, the particle size of plant sterols is reduced as much as possible by crystallization or dispersion technology to improve the absorption rate of plant sterols. One of the most difficult derivatives is to make plant sterols a water-soluble derivative that can be directly added to beverages or water to achieve the highest absorption rate. This method requires more complicated chemical synthesis procedures, changes the chemical structure of plant sterols, and requires more consideration in the queen of food. Fores es Medi-Tech Corporation of the United States successfully linked plant sterols to vitamin C and improved its water solubility. This water-soluble phytosterol is positioned as a new drug, and the United States is conducting clinical trials (phase II), and was patented in 2002 (us 2002/0156021). There are quite a few patents for emulsifying plant sterols to form microcells to allow them to be dispersed and suspended in water (US 5,932,562, 1999; US 6,063,776, 2000; WO 02/065859, 2002; WO 02 / 100412, 2002; JP 2002/29 1 442, 2002). So far, the emulsifier formulations for emulsifying plant sterols have not used egg disc lipids or chemically synthesized surfactants. Although these formulas can achieve the emulsification effect, the cost of lecithin is higher, and chemically synthesized surfactants are still Contains a large amount of fatty acids, so we want to develop a more ideal phytol emulsification technology in 1244376. Saponins are natural emulsifiers extracted from plants, such as: tea saponin is extracted from seeds of Camellia oleifera, saponin is extracted from seeds of Sapindus, and Quillaia ) Is the water extract of the ground bark or wood. Yucca extract is obtained from the desert plants in the southwestern United States. Natural saponins can break down oils and oils and alter cell permeability to promote drug absorption, and can change the surface tension of water. It can be the additive of food and flavoured beverage and the promoter of natural foam formation of food. Saponin has been mentioned in many literatures to have a cholesterol-lowering effect, and it is also edible safety. The results of human tests have shown that Yucca saponin extract has a significant cholesterol-lowering effect on the human body. The use of purified natural plant saponin to form a ceremonial plant sterol mixture should have an additive effect on the physiological activity of plant sterols. This emulsification formula is superior to other formulas that use synthetic emulsifiers to achieve emulsification. SUMMARY OF THE INVENTION The inventors of the present case conducted multiple experiments to test the emulsification stability of phytol by different saponins, and dried the phytol emulsion obtained by emulsification by freeze-drying or spray-drying, and observed the drying. The powder is then added to the dispersibility in the aqueous solution. It was found that tea saponin, saponin saponin, yucca extract, patchouli and saponin bark saponin extract (eXtad) can make plants solid: form stable emulsions and water dispersions. In terms of edible safety and emulsification, the saponin and soap bark 1244376 Quillaia extract have the best effect on the overall consideration of powder redispersibility in aqueous solution. Embodiments The present invention discloses a method for emulsifying a phytosterol, comprising the following steps: a) dissolving phytosterol (Phy to sterol) in an oil phase; b) dissolving a saponin in an aqueous phase; and c ) Mixing the oil phase solution obtained in steps a) and b) with the aqueous phase solution to form an emulsion. Preferably, the method of the present invention further comprises: d) distilling the emulsion under reduced pressure to remove most of the oil phase; and e) drying the oil-in-water (〇ii_in_water) emulsion obtained in step d) 'And a plant sterol powder product containing saponin and phytosterol was obtained, which was re-dispersible in the aqueous phase. Preferably, the oil phase of step a) comprises an organic solvent having a boiling point lower than that of water, and the step d) comprises distilling the emulsion under reduced pressure to obtain a water phase dispersion. More preferably, the oil phase of step a) comprises isopropanol. Preferably, the oil phase of step a) comprises a vegetable oil. The oil phase of Chevrolet ' a) contains animal oil. Preferably, the saponin is a natural saponin (SaPonins) extracted from a plant, and is further extracted from a plant of Camellia oleifera, a plant of Sapindus, a plant of QuiUaia, and silk Orchid (Ya this ⑶) is better. The present invention also provides an emulsion of phytosterol, which is prepared according to the method of the present invention. 1244376 The present invention also provides a phytosterol powder product containing saponin and phytosterol, which is prepared by the method of the present invention. Other features and advantages of the present invention will be further exemplified and explained in the following implementation examples, and this implementation example is only used as an auxiliary description, and is not intended to limit the scope of the present invention. Example 1: Table 1 shows the weight percent of the ingredients used to prepare the phytosterol emulsion. Table 1 Ingredients by weight% Phytol 2.73 Fatty acid propylene glycol ester 2.73 Rapeseed oil 29.11 Saponin 13.09 Fructose 26.17 h2〇26.17 First, first prepare an oil phase solution: 2.5 grams of phytosterol (β-maiger sterol (sitosterol), also known as 24 β-ethylcholesterol, with a purity of 40/0 packs, δ cabbage is obtained from campesterol and dihydrobrassicasterol (available from Sigma) and 2.5 grams of fatty acid propylene glycol ester (propylCDe) Glycol monostearate 90 (referred to as PGMS 90, whose HLB value is 3.4) is dissolved in 26.7 grams of rapeseed edible oil (Taitang canola oil); and then a 1244376 aqueous phase solution of tea saponin (Teasaponin, Sanshui) is prepared. Organic Technology Co., Ltd.) 'and 24 grams of fructose (Tongsu Fengnian Fructose), add μg of deionized water evenly so that after the extraction, the oil phase solution and the water phase solution are at 80. His Majesty used a magnetic stirrer to vigorously mix it 'to achieve a homogeneous and effective mixing effect. After a seven-day observation period', it was found that the emulsified aqueous solution of phytosterols remained stable. Repeat the aqueous solution preparation step. Instead, saponin (Sapindaceae, Sanshui Organic Technology Co., Ltd.), Yucca extract, and bark saponin extract (GanidaInt., USA) can be used instead of tea saponin. Experiment It was found that the four kinds of four hormones used could make the plant sterols reach a stable emulsified state. Example 2: In Example 1, artificially synthesized surfactant fatty acid propylene glycol (propy ene glycol mon〇stearate 90) and fat were used to dissolve the phytosterols. In Example 2, we switched to heating Iso-propanol to dissolve phytosterols. 5 grams of plant sterols were dissolved in 100 mL of isopropanol at 60 ° C. Then 5 grams of various saponins (tea saponin, saponin saponin, yucca saponin, yucca extract and soap bark saponin extract) were separately dissolved in 100 mL of 60 ° C water instead of the water in Example 1. Phase solution. Similarly, the oil phase solution and the water phase solution are stirred vigorously at 600 ° C with a magnetic stirrer to achieve a uniform and stable mixing effect. The isopropyl alcohol was substantially completely removed from the mixed emulsion with a reduced pressure concentration device, and the residual water phase (oil-in-water emulsion of plant-sterol-oil-in-water emulsion) was observed for the Decentralized stability. After a seven-day observation period, it was found that the emulsion of the phytosterol remained stable. After freeze-drying the aqueous dispersion of phytosterol, micronized powders of different pheromones and phytosterols can be obtained. Then add this dried product back to water, and observe the dispersibility of the powder product after adding it to the water solution after drying. The results are good with tea-containing compounds and phytosterols and soap-containing bark extract and plant solids. Water Dispersibility of Known Private Products 12

Claims (1)

1244376 Patent application scope: 1. A method for emulsifying phytosterols, including the following steps: a) dissolving phytosterols in an oil phase; b) dissolving a saponin in an aqueous phase And c) mixing the oil phase solution obtained in steps a) and b) and the aqueous phase solution to form an emulsion. 2. The method according to item 1 of the patent application scope, further comprising: d) distilling the emulsion under reduced pressure to remove most of the oil phase, and e) the oil obtained in the dry refining step d) • Yu-emulsify the liquid to obtain a plant sterol powder containing saponin and solids, which can be re-dispersed in the aqueous phase. 3. The method according to item 2 of the scope of patent application, wherein the oil phase of step a) comprises an organic solvent having a water point lower than that of water 'and the step ❼ comprises distilling the emulsion under reduced pressure to obtain a water phase dispersion. 4. The method according to item 3 of the patent application, wherein the oil phase of the step step comprises isopropanol. Such as applying for the scope of patents! The method of clause, wherein the oil phase of step about comprises a vegetable oil. As claimed, the method of patent enclave i, wherein the oil phase of step &) 13 1244376. I contains animal oil. 7. The method according to any one of claims 1 to 6 of the patent application scope, wherein the saponin is a natural saponin from which a plant is extracted. 8. The method according to item 7 of the patent application scope, wherein the plant is selected from the group consisting of Camellia oleifera, Sapindus, Quillaia, and Yucca Formed by the ethnic group. 9.-Emulsion of plant sterols, which is prepared according to the method of item i of the scope of patent application. W · —A plant containing saponin and phytosterols was prepared according to the method of applying for the fecal brake dream net #U ^ ^ final product, the monthly patent enumerating items 2, 3 or 4. 14