TWI243893B - Fluorescence testing analyzer - Google Patents

Abstract

A fluorescence testing analyzer utilizes a laser light radiated by a laser source to go through a first collimator, a polarization beam splitter and an objective lens so as to radiate to an object to be tested and correspond to a radiating testing fluorescence. The laser light is received and converted into an output signal to carry out an analysis processing of fluorescent signal after further going through a first optical filtering module, a second collimator and an optical sensor. The analyzer is characterized by placing the objective lens on an actuator such that the actuator can be fine-tuned to make the objective lens reach the optimal focusing position in order to measure more accurate data.

Description

V. Description of the invention d) [Technical Field to which Mao Ming belongs] t " ^ Ming Department, with milk — domain, especially f2, a detection device for biomedical related fields [prior art] An adjustable objective lens position Fluorescent detection analyzer. Major technologies such as 5 students and 11 students ’with the pace of world progress and the continuous rise of the research and development of the conductor industry’ have led to a higher level of related electronics. The x exhibition is fast, and the research on biomedicine is also more advanced. Among them, the conventional detection method of biomedical φ φ detection technology is the focus of current research. On-chip, and / The optical disk device with the data layer reads the light of Si at the same time for irradiation, and then uses the data layer gas number of the read layer of the optical disc = the fluorescent signal radiated by the sheet, and the signal of the data layer of the optical disc. Then the data processing unit passes the fluorescent signal and the glory signal of the biochip in the US patent Ιί format. Device ", ie, the optics required for the screening of biomedical wafers for Jielin No. 6 2 0 6 6 0 for biomedical wafer towels, for detection-specimens, clothing I op 11 ca 1 sie ν 1 ng apparatus) ° Opto-mechanical devices are usually selected by two teachers and devices "and common fluorescent detection analyzers. The detection mechanism (that is, the light collecting element) and a receiving mechanism ^ = Composed of parts. The detection mechanism is to guide the light emitted by an excitation light source through the objective lens (〇bject 丨 V ei ens), etc., so that the optical signal is focused on an inspection unit, and a light detector is used. (Examination 〇 Detect〇r; ⑼ order the screening device of 1238993 pieces of optical information ”or general fluorescent elements (such as microscope objectives, objectives > larger volume, so when these lights due to different optical elements It affects the result of the measurement during the positioning between the measurement time. When measuring the time and the comparison between the data, it measures the fluorescence signals at several different positions. At the same time, it measures the fluorescence signal settings at different positions or the error in the assembly of optical components. Fluorescent signals, and some light sources ca n’t detect personnel can only capture a rough data can only be ignored, these will be the test institutions in the light detection analyzer, the error is related industries urgently need to solve this invention to provide a The fluorescence detection analyzer will focus the distance. In this way, the operator will not be able to detect the fluorescence emitted by the object to be measured. 5. Acceptance of invention description (2). Instrument or photomultiplier element is assembly error, and the value of the operator is usually on the number to save the actual value. However, at that time, due to the difference, some fluorescent signal values may be measured, and for some causes Therefore, the experimental error, such as reducing the problem of each optical element. [Summary of the Invention] In view of the fact that the setting position of the focusing objective lens is the best, there are a large number of "biomedical crystals, which contain light tubes." Decrease, to save the time of the same measurement during the operation of the specimen, there are several light sources that can be detected by the fluorescence reaction position light source. Therefore, the reason for the large number of operating errors. How to simplify the assembly of the entire fluorescence The actuator is located at the actuator to adjust the measurement of the objective lens by this actuator to obtain more accurate experimental data. Errors in assembly and positioning, and the measurement signal may only capture and obtain a rough

Page 7 1243893 V. Description of the invention (3) Value situation. A fluorescence detection analyzer disclosed in accordance with the present invention mainly includes: an excitation light source, a first collimator (c ο 1 1 imat 〇r), a polarization beam splitter (Polarization Beam Splitter), and a first optical filter Module, objective lens, actuator, second collimator lens, and light sensor. The excitation light source is used to emit an excitation light. The first collimator is disposed on one side of the excitation light source to receive the excitation light and convert it into a parallel light. The polarizing beam splitter is disposed on one side of the first collimator to receive the parallel light ’and reflect it, and the light exiting edge of the polarizing beam splitter

The edge has a first optical filter module for filtering optical signals of different wavelengths later. This actuator reverses this long detection beam, and is equipped with a straight lens and an objective lens to make the digital microscope and its light ring, or to mix it with the objective lens. Transmitting this pole prevents the edge light from passing out during the measurement of the excitation data. The second collimated light at the specific wavelength side is matched with the polarized light. The actuating light passes through the parallel light and the detection of the spectroscope light and other accuracy. The first light is used to obtain the detection fluorescence detector. The spectroscope is used to make the objective lens irradiated by this objective lens and then the fluorescence is passed through the background light or. Therefore, the filtering mode is correct for the first light. The light is focused. The received side is set up to reach the best focus position and focus on the object to be measured. It will radiate a specific wave &#mirror into parallel light and stray light enters the light sensor and shadow. The present invention uses a polarization beam splitter to emit light and other background measurement results. The Last of the Evidence Wave Module

The side is converted from the detection fluorescence set in the second quasi input to

1243893 V. Description of the invention (4) Signal output for analysis and processing of fluorescent signal. In order to further understand the purpose, structural features, and functions of the present invention, detailed descriptions are given in conjunction with the drawings as follows: [Embodiment] Please refer to "Figure 1", which is the fluorescence detection analyzer 1 of the present invention 0 0 A system architecture diagram of the first embodiment. This first embodiment is used to perform measurement of a single object to be measured. It includes: an excitation light source, a first collimator lens, and a polarization beam splitter. The first optical filter module 31, the objective lens 40, the actuator 50, the second collimator lens 60, and the light sensor 70. This excitation light source 10 is used to emit an excitation light, and many types of it can be implemented. Most of the commercially available products use gas lasers and continuous-spectrum mercury lamps. However, this type of light source module is expensive and the life of the mercury lamp is short. Therefore, in this embodiment, a laser diode (Laser D10) with a single wavelength and a similar function is selected as the excitation light source 1 〇. In the following, an excitation light with a wavelength of 450 nm is taken as an example for illustration. Of course, the wavelength of the excitation light source 10 needs to be different from the dye added to the object under test 80, so that the object under test 80 emits detection fluorescence with a specific wavelength. Therefore, the present invention is not limited to using only the wavelength It is an excitation light source 10 at 45 nm.

The first collimator 20 is disposed on one side of the excitation light source 10 to receive the excitation light emitted by the excitation light source 10 and convert the excitation light into a parallel light or a concentrated light. '' This polarization beam splitter 30 is set on one side of the first collimating mirror 20 to receive the parallel light output from the first collimating mirror 20, and the parallel light is transmitted from a 45-degree plane.

Page 9 1243893 V. Description of Invention (5) Reflected. The polarization splitting filter module 31 is used as the light output edge of the first optical wave module 31 lens 30. The number of shapeable wave filters depends on whether the objective lens 40 is a type. The objective lens 40 is actuated to the top of the sound factor. This wavelength is long, ten (nW). The original allows the searched number to reflect the background light. This is possible, and is located in the refracted post-reflection beam splitter 30. The mirror 40 is set on a device that performs a small distance. This excellent focus position, the test object 80 detects the fluorescence through light, and the nanometer light signal measures the fluorescence and then returns to the polarization fluorescence detection fluorescence through the object optical path. However, to prevent or stray light from entering Light perception through the first optical filter

The light-emitting edge of the optical mirror 30 has a filter for filtering optical signals of different wavelengths after the first optical, but a coating method is used to make a polarized beam split into one or more layers, and the filter filters different measurements for users Demand. The aspherical objective lens is placed on the excitation light path of the polarization beam splitter 30, and the parallel light from the objective lens 40 is focused on the object 80 to be measured. The actuator 50 is used for It will be adjusted (in this figure, the position of the mirror 40 on the z axis can be adjusted). This actuator 50 can be used to fine-tune the element of the objective 40 position. The actuator 50 fine-tunes the position of the objective lens 40, so that all the optical signals are focused on the object to be measured. After the irradiation of the 80 signal, the wavelength with a specific detection fluorescence is larger than the excitation light. However, Its light intensity is only a few tens of nanometer mirrors 40. The search beam is converted into a parallel beam and passes through the light mirror 30. At this time, the polarization beam splitter 30 will be completely incident through. At this time, the excitation light source is multi-wavelength 4 The remaining excitation light of 50 nanometers and other measuring devices 70 affect the accuracy of the measurement. Because the wave module 31 will excite light and other background lights or

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V. Description of the invention (6) ΐ i ί ϊ ’to get a more accurate measurement result. ❿ In order to achieve better learning tears, the light emitting edge of the T-polarizable beam splitter 30 can be coated with a multilayer film of light & and. . ’To filter out unwanted light signals as much as possible. Placed in a larger range and weaker energy 'cause & use the focus to make ί !!: The second collimator 60 on the side of the module 31 will detect the light gate and lift it here for easy detection And receive. Connected: I: Set the second collimator lens 6. The light sensor 7 on one side analyzes it. The light is converted into an output signal ‘卩 for a fluorescent signal to actuate to cry 5Π’: The light sensor 70 outputs this signal for processing, and then returns

The gas collapse eyepiece 70 can be a photodiode (A) or a snow-type sensor (Avalanche pH 0 AM). The instrument is controlled by the operation controller 100. The method of moving the force sensor 50 is described below. This is the fluorescence detection and analysis. First, input—the reference signal to the actuator 50. This reference is the flood number.疋 Used as a reference when adjusting the actuator 50 to the optimal focus position. Tian Tian: When the dynamic 50 receives the output signal returned by the light sensor 70, the comparison between the test signal and the output signal can be used to adjust the direction and position of the actuator. And the objective lens 40 is adjusted to an optimal focusing position.

As shown in Figure 2, "The second figure" is a system architecture diagram of the first embodiment of the fluorescent detection analyzer 100 according to the present invention. The structure of this second embodiment is the same as the first embodiment. Similar to 'However, part of its excitation light source 0 uses the light emitting diode 11 to emit excitation light. A set of spatial filters (Spacial Filtei_) are added between the light emitting diode 11 and the first collimator 20 i20 is made into a point light source, and a second optical filter mode is plated on the light entrance edge of the polarization beam splitter 30

Page 11 1243893 V. Description of the invention (7) Group 9 0 ′ to select the applicable light source frequency band. Please refer to "Figure 3", which is a system architecture diagram of the third embodiment of the fluorescent detector 100 of the present invention. The addition of this third embodiment is the same as the first embodiment, but the first In the embodiment, only once ^ " Dan ^ 检测 detection fluorescence emitted by the test object 80 radiation; and when the operator measures the detection fluorescence signal emitted by a number of test objects 80 in a wide range, day and day ^ The optical architecture of the three embodiments performs detection of fluorescent light.第三 This third embodiment basically forms a plurality of groups of the first embodiment to form the fluorescent detection analyzer 100. This third embodiment uses the same test optical path and optical element as the first embodiment. In order to simplify the optical element in this third embodiment, the number of the 'fluorescence signals' is measured by measuring the measurement of 10 ^ kg and 3 radiations of multiple objects at the same time. Therefore, in the second embodiment, the original polarities are changed. The chemical beam splitter 30 is made into a long polarized beam splitter module 丨 丨 0 to be directly placed into the optical anisotropy. The original first optical filter module 3 丨 was also fabricated on the light emitting edge of the polarization beam splitter module 110 using the coated square. In this third embodiment, a laser diode or a light emitting diode can also be used as its excitation light source 10, and when a light emitting diode is used as its excitation light source 10, it can be used with a second optical filter module by itself. 90, and its structure is not repeated here. The above are only the preferred embodiments of the present invention and are used to limit the scope of implementation of the present invention; that is, all equivalent changes and modifications made in accordance with the patent application park of the present invention are within the scope of the present invention patent. Covered.

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1243893 Brief description of the figure 1 is a system architecture diagram of the first embodiment of the fluorescence detection analyzer of the present invention; FIG. 2 is a system architecture diagram of the second embodiment of the fluorescence detection analyzer of the present invention Figures; and Figure 3 are system architecture diagrams of a third embodiment of the fluorescence detection analyzer of the present invention. [Illustration of Symbols] 10 Excitation light source 11 Light-emitting ^^ Polar body 20 No .- ^ Collimator 30 Polarizing beam splitter 31 First optical filter module 40 Objective lens 50 Actuator 60 No.-Collimating lens 70 Light Sensor 80 DUT 90 Second optical filter module 100 Fluorescence detection analyzer 110 Polarizing beam splitter module 120 Spatial filter

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Claims (1)

1243893 Let the factory-type fluorescence detection analyzer be used to irradiate a test object and receive one of the radiation corresponding to the test object to detect fluorescent light, which includes: an excitation light source for emitting an excitation light; ^ A first collimator is disposed on one side of the excitation light source to receive the excitation light and convert the excitation light into a parallel light; a polarization beam splitter is disposed on the first collimator On one side, the parallel light is received and reflected, and the light exiting edge of the polarization beam splitter has a first optical filter module; the mirror is configured to illuminate the parallel light and then pass through the first side to receive the light. If you input the signal first, you should actuate it. 3. If you apply the excitation light to an objective lens, it is mounted on one side of the polarization beam splitter, and the object is placed on the actuator. Yes, twice. The objective lens focuses on the test object, and the test object radiates the detection fluorescent light having a specific wavelength through the parallel radiation. The detection fluorescent light is converted into a parallel beam and penetrates the polarization beam splitter. A _light · learning_wave module is used to filter light signals of other wavelengths; the younger collimator, which is set on the first optical filter module to make the right and left corners, 5'of the specific wavelength The detection of the fluorescence focus; and /, the deleter is set on one side of the second collimator lens, after the focus, 1. Please patent two private test fluorescence, and convert it into an output signal . The fluorescence detection analyzer described in the second item of Erqianwei, where the system is " = Τ7 π. The broken L is to the actuator, and when the actuator receives the input QtJ-, * 5. » -Must compare the reference signal with the output signal to adjust the size of the movement + γ ^ J ^, the direction, and its displacement. = The fluorescence detection analyzer described in item 1 of Zongweiwei, where the original system is a laser diode. No. 14 Wei's Patent Scope As described in Item 范围 of the patent application scope, the light source analyzer is a light-emitting diode. A. For example, the luminescence detection analyzer described in item 4 of the patent application, and the fluorescence detection analyzer described in item 4 of the patent application range, π. A spatial filter is provided, which is arranged between the light-emitting diode and the first disaster including rj ′ to focus the excitation light into a parallel light. , Fluorescent detection analysis of the straight lens as described in item 1 of the scope of patent application. The δ objective lens is an aspherical objective lens. Among them, the actuator of the fluorescence detection analyzer as described in item 1 of the scope of patent application is a voice coil motor. Me, which one of the nine. The first optical filter module of the fluorescence detection point as described in item 1 of the scope of patent application contains more than one optical filter, where W // 0, ^ Please, Lee Fan 81 The fluorescence detection _ light detector described on page 1 is a light-receiving diode (pD). , 1 and 1 · // Fluorescence detection analysis described in item 1 of the patent scope of the Leqing # Two senses and one residual device is-avalanche type light sensor (exhaust. Knife analysis _ ， $ kin cocoon light 祆/ The analyzer is used to illuminate an object, and receive the testing labor of each pair of the object to be tested_ Wang Straight Line, which includes: Jin pairs "shooting-more than one lined up in a straight line The excitation system emits more than one excitation light; ", 'Each of the excitation light source mirrors is respectively placed on the first collimator lens of the Λ straight line f, and each of them is placed on each of the excitation light sources. 4 brothers on one side should be Receive from each page 151243893 VI. Patent application scope Excitation light and convert each excitation light into more than one parallel light; A long polarizing beam splitter module is installed in each One side of a collimator lens to receive and reflect each of the parallel light, and the light emitting edge of the polarization beam splitter module has a first optical filter module; more than one objective lens arranged in a straight line, and is assembled on the One side of the polarization beam splitter module, and each of the objective lenses is respectively disposed on one or more actuators, and the reflected parallel lights are focused on each of the objective lenses by fine-tuning the actuators. Each of the objects under test is irradiated with the parallel light to irradiate each of the detection fluorescence with a specific wavelength, and each of the detection fluorescence is converted into a parallel beam by each of the objective lenses and penetrates the Polarized beam splitter module, and the first optical filter module is used to filter light signals of other wavelengths; one or more second collimator lenses arranged in a line are arranged in one of the first optical filter modules Side to make Each of the detection fluorescent light having the specific wavelength is focused; and _ more than one linearly arranged light sensor is disposed on one side of each of the _ second collimating lens to receive each of the detection fluorescent light after focusing respectively Light and convert it into more than one output signal. 1 3. The fluorescence detection analyzer according to item 12 of the patent application scope, wherein a reference signal is first input to each of the actuators, and when each of the actuators ® receives each of the output signals By comparing the reference signal with the output signal, the direction of movement of each actuator and the magnitude of its displacement are adjusted separately. 1 4. The fluorescence detection analyzer described in item 12 of the scope of patent application, wherein P.16 Among them, the lighter the lighter, the lighter the more accurate the first light. 'Wherein' where 'where 〇' where 'where 1238993 6. Patent application scope The excitation light source is a laser diode, 1 5 · The luminescence detection analyzer described in item 12 of the patent application scope, the excitation The light source is a light-emitting diode according to 16 · The glory detection analyzer described in item 15 of the scope of patent application, which contains a second optical filter module located at the entrance edge of the polarization beam splitter. 17 · The fluorescence detection analyzer as described in item 15 of the scope of patent application, which includes a spatial filter disposed between each of the light-emitting diodes and each of the straight mirrors so that each of the excitation light is focused and Converted into each of the parallel 1 8 · The fluorescence detection analyzer as described in item 2 of the patent application scope, the objective lens is an aspherical objective lens. 19. The fluorescence detection analyzer as described in item 12 of the scope of Shen Qing patent. The actuation is a voice coil motor. 2 0 · The fluorescence detection analyzer as described in item 12 of the patent application scope. The first optical filter module contains more than one optical filter 2 1. As described in item 2 of the patent application scope. The fluorescent detection is divided into the photo-sensor is a light-receiving diode (PD). 2 2. The fluorescent detection analyzer described in application ^ y ^ I I and the first 12 items of this application. The light sensor is A, and the instrument is an avalanche light sensor (APD). Page 17