TWI226369B - Solid fermentation device for mass production of Metarhizium anisopliae - Google Patents

Abstract

A solid fermentation device for mass production of Metarhizium anisopliae mainly includes a solid state fermentation tank, a delivery device, an inoculation device, a temperature and humidity control device, a separation device, and a control unit. After soaking, a culture medium is transported through the transport platform of a delivery device to a solid fermentation tank. Next, the culture medium is cooked by steaming in the solid state fermentation tank, and then inoculated. The fermentation tank is controlled for its environmental conditions by the temperature and humidity control device to produce spores, which are delivered through the pipeline of the delivery device to a separation device, and then subjected to blowing and oscillation to mass produce Metarhizium anisopliae spores. The Metarhizium anisopliae spores produced can be used as a fungal insecticide and other applications.

Description

1226369 Case number 91114295

V. Description of the invention (1) [Technical field to which the invention belongs] The present invention refers to a solid fermentation device for mass production of black fungus, especially a specific device that enables Black Edge to reach commercial mass production. [Previous technology] ^ First press' In the past, research on the use of microorganisms to control pests mainly focused on viruses and bacteria, and entomogenous fungi were less attractive, although there were as many as 750 species of entomogenous fungi with control potential. However, it was not until 1981 that an entomogenous fungus was officially registered and commercialized in the United States. In fact, it was first discovered to be parasitic to the contact biome Beauveria bassiana. At the end of the 19th century, the Midwestern United States used white worm to control B1 issus leucopterus. In the past, some people thought that the effect of entomogenous fungi on controlling pests was relatively unstable, but recently, due to the development of epizootiology and the selective use of highly pathogenic fungi, there have been many successful examples of pest control. Commercialized white colony-Boverin to control Colorado potato beetle (Leptin〇tarsa decemlineate); Brazil uses black bacterium (Metarhizium ani sop 1 iae) to control sugar cane and Mu Mo foam; World Health Organization (WH〇) also sponsored research Black colonies successfully tested for mosquito control; St Samoa used black fungus to control coconut palm rhinoceros beetle; Verticillium lecanii was used to control aphids in greenhouses in the UK; Cu 1 icinomyces c 1 avosporus was used to control mosquitoes in Australia; Hirsutel la thompsoni was registered in the United States in May 1981. It was the first commercial worm in the United States to control Citrus rust mite. Second, the application of worms in Taiwan has been Use of white fungus to control pine

1226369 amendment 911142Q5

V. Description of the invention (2) An example of the success of the caterpillar. Recently, the owner of the genus Nigella spp., On coconut palm red-, pathogenic Plutella xylostella, Phalaenopsis sibiricum, Twill night: 荽 >: view of the turtle and delicacy family vegetables The pathogenicity of Muz is quite strong, == White: Pseudomonas and Paecilomyces will have vast commercial potential for silver mixture. Tan F is developed as a fungal insecticide to use a hot PP bag space bag culture; type hair :: The production technology of black fungus is only available in rice grain culture medium, the method of spores ~ 2, 3 spore production field test black maggot Bacteria biological control; and for testing or small-scale to meet commercial mass production, & & er ΐ δ tadpole application needs, but obviously can not do its job to find a large and convenient solid fermentation tank [Summary of the Invention] As mentioned above, for the development of Nigella spp. ~ There is a vast potential for commercial sacrifice, and the medium # λ, M 4, and bifurcation agent technology has reached Π 2: 1 :; The production target, and consider the mass production of black fungus. The main goal of f Ming: Γ: ΐΓ, =: the basis of automated commercial production, also Hi main / equipment, the fermentation main tank adopts fully automatic process and immersion. The heavy axe is automatically transported into the main fermentation tank, and then the two substrates are used in the main tank through temperature control, thirst, and wet spraying. After the spores are produced, they are distributed from the main tank to the vibrator and cyclone to spore The collector completes the entire process. And fermented throwing Shida j / spore drop-using non-closed

Page 6 wind, the black spores are collected by a strainer, and the strainer also has a vibrating machine \ 1226369 Amendment V. Description of the invention; two mouths and conditions that can keep free of bacteria, and the other one under the main fermentation tank ^ ^ tCleaning channel. In addition, the inoculation source of Phlebium nidulans is hooked, and the present invention includes a two-turned substrate, and the uniform inoculation effect "will be used to achieve the goal of commercialization of commercial production. The main purpose of the invention is to establish a commercial burial technology for microbial pesticides to produce and build private # 7 Μ Μ Ω to produce Μ 礓 _ stuffing plants with the potential for the development of biological agriculture, in order to establish large-scale and inexpensive θ A painful person. The technology and mass production system of the black letter function for viewing and saving money [Implementation] Now, to make your reviewing committee take the invention one step further-the following examples are used to illustrate it. ', Please refer to the first figure for the review member. The "solid fermentation equipment produced by the black cricket" of the present invention, the main operation process of the equipment is the substrate reading, feeding into the solid fermentation tank, cooking, inoculation, and controlled humidification Mix with J wind, output solid fermentation tank after filling with spores, and continue to separate the substrate and spores through the air blowing and shaking eight separation procedures to obtain the mass-produced spores of Nigella spores. The knife is cultured and input Solid state fermentation tank Brine and spray inoculation, temperature controlled humidification and ventilation, and regular feeding of the culture medium to full spore production, and then output to the solid fermentation tank, and the separation process of ^ wind, ^ swing separation matrix and spores, and the yield According to the actual operation, the culture conditions are as follows (the substrate is rice grains.) (A) When the substrate is soaked, the water temperature is 26 ° C ± 1 ° C, and the soaking time is Γ 12 o'clock; initial moisture content of immersed substrate: 50 ~ 60% ', ΐ · β1M 1226369 ^ _ Case No. 911U9.QR V. Description of the invention (4) (b) During basic culture,' Initial thickness of substrate culture kg; Time ·· 10 to 14 days. (C) Temperature control during each period of the culture period, 耱 1 ° C; Temperature of the culture period: 2 5 ° C ±] Temperature of the public Λ stage: 2 6 ° C Soil 35 ° C ± 1 ° C. —, Spore drying temperature: (d) humidity control in each period during the cultivation period, etc., 1%; humidity during the cultivation period: 90% ± 1%. Day's humidity · 97% soil (e) Ventilation · 100 mmAQ; stirring rate · substrate (kg) · inoculation volume (liters) = 10 · · 丨. ΓΡΠΠ '· inoculation ratio: In addition to the rice grains, rice, bean curds, and other inexpensive and carbon- and nitrogen-rich raw materials are crushed. 1 The initial moisture content of the bean and jade soaked matrix is slightly different, and the rest are homogeneous. Please refer to the second and third steps for solid fermentation tank 1, conveying device 2 and inoculation equipment, including control device 4, separation device 5 and control unit 6, of which Tan wet solid fermentation tank 1 (see also Figures 4 and 5) are hollow buckets designed in a non-style design, which includes a central axis: 1: 2 agitating shafts 1 3, 2 lifting components 1 4 and several power sources 丄 5, tank body In addition, it has a feeding port 16, a discharging port 17, and a 'window 18 (see the fourth figure) and a slot door 19, and the central axis i 1 is positioned in the center of ^, and the bearing network platform 1 2 It is pivotally connected to the bottom of the central axis 11 and is suitable for supporting the substrate 7. Its dual power source 15 can be rotated by the action of the power source 15. The rotary shaft 13 is located on both sides of the central axis 1 1 '. By the movement of the lifting component 1 4 fixed in the tank body * 溆 1 5 and can be rotated, which-^ fell, the other stir axis ^ has screw = row---- ^ 1111

cm soil cm / 30 closed platform lift source 1 4 power source red 1 O j-rt-

Page 8 1226369 _Case No. 91114295_Amendment of the month of the year _V. Description of the invention (5) The stirring rods 1 and 2 in column 5 are used to stir and turn the substrate 7, and the stirring shaft 13 passes through the groove body. The tank system is provided with a long tank 100 and a channel 1 0 1, and the stirring shaft 13 is fixed there with a long cover 1 3 3 penetrating the track 1 0 1 and covering the long tank 1 0 0 , And the stirring shaft 13 is pivoted on the central shaft 11 at the central shaft 11 at a separate straight rail 1 1 1, so that the stirring shaft 13 can slide up and down when it is actuated by the lifting assembly 14. And still cover the long slot 100 (as shown in the sixth figure), and the lifting assembly 14 has a power source 15, a base 141, and a reference shaft 142, and the base 141 and the power source 15 of the stirring shaft 1 3 are synchronized to rise Or descend, and the basis for its rise and fall is that another power source 15 is actuated to rise and fall along the reference axis 1 4 2 and the rise and fall of the stirring shaft 13 can be adjusted according to the amount of the substrate 7 and the amount of the substrate 7 to facilitate turning Stir (see the sixth figure), and the bottom end of the solid fermentation tank 1 is also provided with a drainage pipe 1 10. In addition, the discharge port 17 can discharge the fully spore-producing substrate 7 so that the substrate 7 is passed through the shaker 8 again. Shock to separate spores and substrates 7. Furthermore, the solid state fermentation tank 1 is provided with a steam tube, and the steam in the steam tube can be generated by a boiler (where the boiler and the steam tube are not shown in the figure), so that the high temperature pair of steam using the steam tube is sent into the solid fermentation tank 1 The substrate 7 is first sterilized by cooking. The conveying device 2 mainly has a conveying table 21 and a plurality of pipelines 2 2 (see the second, third, and seventh drawings). The conveying table 2 1 conveys the substrate 7 to the feeding port 16 after soaking, and Into the solid state fermentation tank 1, the other pipeline 22 includes the main pipe 221 and the auxiliary pipe 2 2 2. The main pipe 221 is connected between the top of the solid state fermentation tank 1 and the separation device 5, and the auxiliary pipe 2 2 2 is connected to the main pipe 221. Set it out. Inoculation device 3 with a bucket 3 1, a power source 3 2 and pumping

1226369 --- ΆΜΜ295__ 生 _ 月 说 你 γρ V. Description of the invention (6)-一 " " ----- Pipe 3 3 (see the second picture), the power source 3 2 series will be contained in the bucket 3 The black fungus spores are sprayed into the solid fermentation tank i through the suction pipe 3 3: the substrate 7 is sprayed and inoculated after cooking. The temperature and humidity control device 4 has an air filter 4 1, a duct 4 2, and a humidity damper 4 3 (see the second figure), so that the air is sent through the air filter 4 2: the filter and the duct 4 are sent in a round, Through the temperature and humidity regulator 4 3 temperature-controlled humidification and ventilation, the wind is introduced into the solid-state fermentation tank 1 to make the environment-controlled conditions of the substrate 7 reach a state. % Leaven (see section 1), the base matrix of the drum part collects 5 2 inner top, and the top 5 2 1 up 2 4 and uses the tube 5 2 5 tube 5 2 5 to complete the spore-power source 5 2 6 to 7 to the duct 7. When the positive separation device 5 is maintained, there is a blast bucket 5 1 and a oscillating box 5 2 (Figures 2 and 8). A substrate collection box 5 is provided below the blast bucket 5 1. The blast bucket 5 1 is connected to the pipeline 2 2. The main pipe 2 2 1 causes the spores and the substance 7 to be drawn here by a blower, so that the heavier substrate 7 falls to the box 5 1 1, and the light spores enter the connected shock box side, and the shock box 5 2 is below A spore collection box 5 2 1 is provided with a shaking frame 5 2 2. The shaking frame 5 2 2 and the spore collection box each have a flat plate 5 2 3, and the flat plate 5 2 3 has holes. The collection cloth barrel 5 2 5, and the upper end of the collection cloth is shaken by the vibration rack 5 2 2, so that the spores can be easily introduced from above the collection cloth, and then enter the spore collection box 52 ，, the goal of spore separation and collection. In addition, The shaker 5 2 2 is the one who is actuated. In addition, on the top of the oscillating box 52, another exhaust pipe is provided to discharge exhaust gas, and the air duct 5 2 6 also extends a pipe 5 2 4 2 and the auxiliary pipe 2 2 2 of the pipe 22 is connected to the pipe. 5 2 Of course, a valve 9 is provided at an appropriate place for regulating and opening and closing in a timely manner, and is often operated or conducted during ventilation and cleaning. V. Description of the invention (7) One; ^ ϋ Early Wu 6, mainly a computer θ 1 with PLC environmental control mechanism and phase 6 2 to control the operation of each device and unit by connection. The same energy ^ soil, the substrate 7 after culture and soaking is mainly sent to the bar 2m through the conveying table 21 "" after cooking, inoculation and temperature and humidity control of the temperature and humidity control device 4, and then sent to the separation line 22 The device 5 enables the control of the worms: The black spore-producing spores are used as an effective fungal insecticide in the two institutes "and can be implemented concretely. The weeping dagger is the main function of the present invention. In addition, the matrix 7 can be cheap, carbon- and nitrogen-rich raw materials such as rice grains, crushed rice grains, and Da Ming Shi, and the actual production yield shown in Figure 9 of this hairpin for certification. Compare their base prices for actual cost analysis. Soil I5 of the invention provided Ϊ embodiment described embodiment, mainly referring to the black Jiang bacteria can be produced by the present amount of ^ Ϊ # ί Ί f amount of 'serve to establish a microbial pesticide product lines, establishing cooking to produce large development potential biopesticide The black fungus fungus is used for biological production of t ^ two cheap t black fungus mass production technology and quantity system. The main member of the committee is' Ke Ku Shi, and the invention A is entrusted to the agricultural science of the Executive Yuan = = Jin bacteria ... This issue has practicability and step i; :: Inter-operational operation is practical and feasible, and we can know that the highly beneficial = the patent elements of the technical idea of the law of nature. However, it is consistent with the development of ^ + newness and progress, and is not the one described by Shuang P ~, it is only the preferred embodiment of the present invention and the scope of the implementation of the patent ^ 2 f invention. That is, where the application is covered in accordance with the present invention.变化… and other changes and modifications, 胄 is the patent scope of the invention 1226369 Case No. 91114295 Month, Day Amendment Simple illustration of the drawing [Simplified illustration of the drawing] The first diagram is a schematic diagram of the working process of the present invention. The second figure is a side reference view of the device configuration of the present invention. The third figure is a top reference view of the device configuration of the present invention. The fourth figure is a partial perspective reference view of the solid-state fermentation tank of the present invention. The fifth diagram is a plan reference diagram of the solid fermentation tank and the lifting assembly of the present invention. The sixth diagram is an operation reference diagram of the lifting assembly of the present invention. The seventh diagram is a plan reference diagram of the feeding and discharging of the present invention.

The eighth figure is a reference plan view of the planar structure of the separation device of the present invention. The ninth figure is a comparison chart of the solid fermentation amount of black fungus in different substrates of the present invention. [Description of Symbols of Main Components] Explanation of Symbols of Components: 1____Solid State Fermenter 1 1 1 ... ... Straight Rail 1 3 • · · .Stirring Shaft 1 3 2. ... Stirring Rod 1 4 ... .Lifting Assembly 1 4 2. ... reference axis 1 6 ..., feed port 1 8 .... Window 1 0 0 ... ... long slot 1 1 0 ... ... drain pipe 1 1 ... central axis 1 2… .. bearing platform 13 1____ spiral blade 1 3 3 .... long cover 14 1____ base 1 5 · · · · power source 17 ... outlet 19____ slot door 10 1___ _track

Page 12 1226369 _Case No. 91114295_ Year, month, and day correction diagram simple description 2 • • • • ¥ m Sending device 2 1 • • Section j Sending table 2 2 • Boxing • • Pipe 2 2 1 • • Supervisor 2 2 2 • · • · Auxiliary tube 3 .. Inoculation device 3 1 Drum 3 2.. Reference power source 3 3 Pumping tube 4 • ·, field / m wet control device 4 1 Air filter 4 2 .. • • Conduit 4 3 Thermo-humidity regulator 5 .. Separating device 5 1 Blast bucket 5 1 1 »» 9 Matrix collection box 5 2 Vibration box 5 2 1 • • • 9 Spore collection box 5 2 2 • Vibration rack 5 2 3 Climbing m 9 Slab 5 2 4 • Spring hole 5 2 5 • ··· Collecting tube 5 2 6 · Air duct 5 2 7 • · .. Takeover 6 .. .. Control unit 6 1 • ·. Computer 6 2 .. • Control box 7. Matrix 8 Oscillator 9 • Valves

Page 13

Claims (1)

12263cake 9liM95. Amendment VI. Application for Patent Scope 1. A solid fermentation equipment for mass production of black fungus, mainly including solid fermentation tank, conveying device, inoculation device, temperature and humidity control device, separation device and control unit. · The solid state fermentation tank is a hollow barrel body, which includes a central shaft, a supporting net table, two stirring shafts, two lifting components and several power sources. The tank body also has a feed inlet, a discharge outlet, a window and a For the slot door, the central axis is located in the center of the slot, and the supporting net table is pivotally connected to the bottom of the central axis, and is suitable for supporting the substrate. It can be rotated by the action of a power source, and the two stirring shafts are located in the center. On both sides of the shaft, it can be rotated by the power source of a lifting component fixed outside the tank. One of the stirring shafts is provided with spiral blades, and the other stirring shaft is provided with spirally arranged stirring rods. A long slot is provided in the slot body at the slot body, and a channel is provided, and the stirring shaft is fixed there with a long cover penetrating through the track f anti-shielding long slot, and the mixing shaft is resting on the central axis. The central shaft is provided with a straight handle, so that the stirring shaft can slide when it is moved up and down by the lifting component, and still covers the long groove. The lifting component has a power source, a base and a reference shaft, and the base and the stirring shaft The source of power rises or falls simultaneously, and the basis of its $ and drop is another power Actuate up and down along the reference axis; the solid fermentation sample is replaced with a steam tube, so that the steam in the solid fermentation tank is steamed and sterilized by the steam of the steam tube; 9 ^ The conveying device mainly has a round table and several tubes The conveying platform is used to transport the base, ', £ > to the feed port after being soaked, and the other pipes include the main pipe and the N pipe. The main officer is connected to the top of the solid fermentation tank and the separation device pipe. It is set up from the supervisor; the sentence and vice inoculation device has a bucket, a power source and a pumping tube, and the power source 1226369 Application scope of patent ---- The black fungus spores contained in the barrel are sprayed into the solid state through the pumping tube to make the substrate spray-inoculated after cooking; & 'machine, = control device, with air Filters, ducts, and temperature and humidity adjustment J l are filtered and transported by air filters, and introduced into solid-state fermentation tanks by humidification and ventilation through temperature and full-adjustment dishes ^ Separation device with blast bucket and shock box, blast The bottom of the barrel is a collection box. The blower barrel is connected to the main pipe to hold the spores; the spores make the heavier substrate fall to the substrate collection box, and the quality: = 子 _, and there is a vibration rack above :: : There is a flat yoke on the top of the collection, and there is a hole on the collection plate of the ancient times, which is used to pass through the collection cloth that penetrates up and down, and the upper end of the collection cloth barrel is shaken from the collection cloth barrel.导入 λ: Zhu Zhizhen I, moving the spores φ, -h into the spore collection box, and the earthquake Γ 'is set on the top of the vibration box-2 ducts ϊϋΓ ΐ Guide tube extension _ take over to the catheter, X the field of the pipe J e is connected to the take over, and is equipped with a valve for timely The control unit is a computer that cooperates with the PLC environmental controller to report to the workers, and is used to control the operation of each device, the unit, and the control box. In the test box, the immersed substrate is sent to the carousel via the rotary table. Solid-state fermentation tank; then sent to the separation device through the pipeline to make the blowing; control = work = bacterial spores. | Dang Er's production of black equipment 2. As described in the patent application scope of the "body fermentation equipment", wherein the sample shaft is raised and lowered because m = 〇 Page 15 1226369 _ Case No. 91114295_ Year Month Amendment _ Sixth, the scope of patent application is small and adjusted. 3. The "solid fermentation equipment for mass production of black fungus" as described in item 1 of the scope of the patent application, wherein the bottom end of the solid fermentation tank is provided with a drainage pipe. 4. As described in the "solid fermentation equipment for mass production of black fungus" as described in item 1 of the scope of the patent application, wherein the discharge port is capable of unloading the spore-forming substrate, and the substrate is oscillated by a shaker to separate the spores from the spores. Matrix. Page 16