TW515706B - LED light source-based instrument for non-invasive blood analyte determination - Google Patents

Abstract

A compact, lightweight instrument for non-invasive blood analyte determination employs a light source incorporating an assembly of LED's interconnected within a thermally stable substrate. A large diameter mixer couples the signal to a fiber optic probe for delivering the signal to a tissue measurement site. Back-diffused light is collected and dispersed across an array of photo detectors in a miniature spectrometer instrument. A high-speed DSP executes an algorithm for predicting concentration of a target analyte, which is output to a LCD display. Instrument control is by means of keypad or voice recognition. High conversion efficiency of the light source results in extremely low power dissipation and virtually no heat generation, making incorporation of the light source and the spectrometer into a single unit practicable. High-speed pulsing of the signal allows application of high-sensitivity, synchronous detection techniques. Speed and flexibility in sequencing LED's allows simultaneous measurement and skin temperature control.

Description

515706 A7 B7

Printed by the Consumer Affairs Bureau of the Intellectual Property Agency of the Ministry of Economic Affairs, X Consumer Cooperative, Ltd. V. Description of the invention () Field of the invention The field of the invention relates to the analysis of analytes in blood by non-invasive methods. More specifically, the present invention relates to a miniaturized instrument that uses a light-emitting diode (LED) mounted on a thermally stable substrate as a light source for non-invasive gold liquid analyte measurement. Description of related techniques Traditionally, clinical trials require invasive methods such as biopsy and bloodletting to obtain blood and tissue samples. The sample is then sent to a central area, such as a laboratory, for subsequent testing and analysis. However, the current trend is toward point-of care testing (in-home testing) or in-home testing. The advantage of this trend is to reduce the time required for the sample to be returned after the test is completed for subsequent follow-up. action. At the same time, this trend has made the sampling process more non-intrusive. Because minimally invasive or non-invasive procedures can reduce or eliminate the need to process blood and tissue samples, they also significantly reduce the risk of biohazards to subjects and operators. The purpose of the non-invasive blood analyte measurement method is to measure the concentration of the target analyte in the blood in a way that does not need to penetrate the skin. Near-infrared light (NIR) diffuse reflectance spectrometry is quite suitable for non-invasive blood analyte measurement. This method uses a low-energy near-infrared light spot (750-2500 nm) to irradiate the body for non-invasive For blood analyte measurement. Near-infrared light interacts with the chemical components in the tissue, part of which is absorbed, part of which is scattered, and then reflected back to the detector. Top detected by the detector Please read first * Read 1 | Back * Side * of * Note 1 Note: Things · Items

The size of the paper is applicable to the Chinese National Standard (CNS) A4 specification (210X 297 Gongchu) 515706: The A7 B7 printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs, the description of the invention () can be used to limit the light intensity -logR / Rs diagram, where r is the intensity of the light reflected by the skin back to the detector 'Rs is the standard reflection spectral intensity of the instrument. In the infrared spectrum, the pattern obtained by the above method is similar to the absorption spectrum with quantitative information obtained by the known interaction between the emitted light and the body composition. Portable and portable non-invasive blood glucose analyzers are available for point-of-care testing and on-site testing. However, the development of such instruments is subject to certain restrictions, such as the light sources traditionally used in spectrometers. Tungsten tungsten bulbs used in most traditional spectrometers have the disadvantages of being too large and energy inefficient. It requires more energy, shorter battery life and shorter lamp life, generates extra heat, and takes longer to stabilize the light source. In addition, traditionally, the light emitted by the light source is transmitted to the measurement area by a continuous optical probe or a space optical configuration design using an array of mirrors and lenses. This design requires a large accommodation space and is very sensitive to vibration. Several embodiments in the prior art are related to light source assemblies for non-invasive optical sampling. For example, U.S. Patent No. 5,27 1,279 (December 14, 993) owned by F. Levinson is an optical mixing device with an optical fiber output. The device is a device that can mix light for use by a spectrometer. There is a mixing rod in the device, which uses many optical fibers to mix the light emitted by the light emitting diode light source fixed to the conductive block. ; The Kunren stick will emit light with different central wavelengths from several light-emitting diodes, effectively mix them into one light, and then divide the light into several single pages. Page 4 This paper applies Chinese national standards (CNS) ) A4 specification (210X297 mm) '' ~ ---- 515706 A7 B7 V. Description of the invention () The light beam passes through the optical fiber. The instrument cannot collect light from a sample, nor can it shape light from a single light-emitting diode. In addition, when the conductive block of the light emitting diode assembly is used as a heat dissipation channel for the excess thermal energy generated by the light emitting diode, an active cooling unit should be provided to maximize the energy efficiency of the light emitting diode. US Patent No. 6,134,458 (December 31, 1991), owned by R. Rosenthal, is an optical probe for chemical measurement of near-infrared light. This patent describes a spectroscopic instrument that includes an external The light probe of the polyhedron illumination ring, the light-emitting diode is placed near the polyhedron. After the emitted light enters the illumination ring, after the illumination ring contacts the desired measurement area on the body, the light can be introduced into the measurement area. , And the light detector is arranged on the same axis as the illumination ring. The device invented by Rosenthal cannot collect light from the measurement area, nor can it mix light from several light-emitting diodes. The light emitted by the light-emitting diode is directly introduced into the probe without the intervention of a mixer for the purpose of completely mixing the wavelengths of all light. The device invented by Rosenthal does not provide any function that can change or adjust the shape of the light emitted by the light-emitting diode, nor does it provide a thermally stable substrate for the light-emitting diode, and the maximum operating temperature of the light-emitting diode. Optimized active cooling system. Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economics. Currently there are spectrometers for measuring blood analytes, such as glucose, but in general, such devices are not designed to be portable. For example, K. Kaffka, L. Gyarmati, I. Vdlyi-Nagy, I. G0d0lle, G. Domjdn, J. Jdko, US Patent No. 5,947,337 (October 26, 1999), which is a method And instrument, to the Chinese paper (CNS) A4 specification (210x297) in page 5 of this paper size 515706

V. Description of the invention (Fast non-invasive method to determine the parameters of blood components β This instrument will emit near-infrared light to the phalanx of the subject's finger, and the transmitted or reflected light can be detected and analyzed to estimate the blood glucose concentration "But Kaffka Et al. Did not interpolate in the patent whether the instrument is a portable or portable instrument. The detected signals can be introduced into the optical fiber using a conventional lens and mirror arrangement design. The space requirements are not suitable for portable instruments. The light-emitting and collection fibers used are separate structures. This also requires additional space to accommodate β M · Block and US Patent No. 5,424,545 owned by Sodickson ( June 13, 1995) is a non-invasive non-spectrophotometric infrared light method for measuring analyte concentrations in blood. The patent describes a non-invasive method for measuring blood analytes. The device uses colorimetric analysis Measure the analyte in the gold solution. The device is not portable or movable. Same as the previous reference. 'Beams are arranged using mirrors and lenses. It is introduced into the optical fiber, so it has the same disadvantages. D. US Patent No. 6,064,898 (May 16, 2000) owned by Aldrich is a non-invasive blood component analyzer, which describes a non-invasive blood component analyzer 'The device provides built-in path length monitoring for subjects with different finger sizes. The device is not portable or mobile. The light source of the device can be a light emitting diode or a light bulb. The device No connection device is provided to direct the light from the light source into the measurement area, so the light is emitted directly near the measurement area, and the air directly enters the space of the sample area. The device for analyzing analytes in blood is not the same as the device with the same function described above Use this page page 6 This paper size applies Chinese National Standard (CNS) A4 specifications (210X 297 public love) (Please read the precautions on the back first to write this page) System 515706 A7 B7 V. Description of the invention () The light-emitting diodes in the column arrangement are used as the light source, and no bottom is provided (please read first (Notes on the reverse side of this page) to provide mechanical support, thermal stability, and electrical conductivity of light-emitting diodes. As for the portable spectrophotometer leaf mentioned in the art, κ Levin, S · Kerem, V · U.S. Patent No. 6,031,233 (February 29, 2000) owned by Madorsky is a portable infrared photometer. This patent describes a portable infrared photometer. The internal space is obtained by removing the optical fiber part and arranging the optical instruments in a row. In order to achieve the purpose of saving space, the light source uses a traditional light bulb to emit light and then pass through sound and light. The ambient / opticla tuning filter A TF is used for wavelength selection. The light filtered by the filter is focused by one or more lenses' and guided through a window to the sample area. Because the device is designed with high space saving in mind, so that the device can be hand-held, a combination of an acousto-optic tunable / wave filter with an optical wavelength synthesizer and an RF amplifier is used. In addition, traditional light bulbs have disadvantages such as poor energy economy, short battery life, and excessive heat generation. Printed by H. Van Aken, A. Kravetz, K. Garde, W. Weber, J. (: 〇): US Patent No. 5,319,437 (June 1994, owned by (10) 7) is a portable photometer, describes a portable photometer, the device uses a traditional light bulb, so there are all the defects caused by the traditional light bulb can be used as a portable non-invasive blood glucose analyzer. The example is R · US Patent No. 5,077,476 (October 17, 2000) owned by Rosenthal's patent is for non-invasive measurement of glucose in blood. ) A4 specification (2ιχχ 297 publicly loved 515706 A7 B7 5. Device description of the invention. The device is a portable non-invasive blood glucose analyzer, which provides one or more light-emitting diodes as pre-selected wavelengths Infrared point light source. Infrared light is injected into the sample area using a focusing lens. However, the light emitting diode array emitted by Rosenthal's invented instrument will have overlapping parts in the wavelength range. The light does not provide a wide signal spectrum.And the space arrangement design of the lens and detector of the device requires a large space, so it is too large as a portable device. In addition, the device does not have a spectrum analyzer and a linear array of detectors. Printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs From the above description, it is known that a long-life, space-saving and energy-saving light source is urgently needed for non-intrusive optical sampling. To meet the above conditions, a light-emitting diode LED, S) as a light source will be a choice, in addition, the light emitting diode is placed on a substrate that can provide current conduction, mechanically for support and thermally stable substrate to become a miniaturized sub-component. It will be the expected way. When the wavelengths of the light emitted by the light source are to be completely mixed and the energy of the emitted light can be normalized, the optical fiber can be used as a device to conduct the light emitted by the light source. Disturbance and space saving advantages. If the operating temperature can be optimized again, the light emitting diode can achieve better operating efficiency. It would be a major technological advancement to deploy a light source assembly in a lightweight and miniaturized device for non-invasive blood analyte measurement. SUMMARY OF THE INVENTION Known for a non-invasive page 8 ^ 15706 Description of the invention () type light-emitting diodes used for optical sampling: Ma 4 red ,,, original components. By combining several or several groups can emit wavelength band overlapping The light ~ polar body gets a wide grinding signal. The light-emitting diode / substrate sub-assemblies 々 spoon is connected with the light-emitting diode which is connected with the wire. The light-emitting diode light source assembly of the refractive lens on the stable object contains a ^ Yiyou body / substrate sub-assembly; χ has a hollow large-diameter optical fiber to allow the signal to be transmitted to the 揸 ^ /, 芏 Bi pin A mixer that passes through the empty center to collect the light emitted by the sample. The light emitting diode / substrate subassembly is arranged on the light emitting diode to provide a space between the light emitting diode and the light emitting diode driver. Current-connected printed circuit boards · Life ^, Yinji, and stable substrates Degrees cooler. In a second specific embodiment, the present invention provides a compact, light-weight instrument that uses the first specific tone / > and the light source assembly in the embodiment for non-invasive blood analyte measurement. Use. The instrument includes at least a light emitting diode light source component and an optical module. The module includes at least a linear array of detector arrays and a mini-spectrum analyzer that receive a dispersion spectrum. Grating of light transmitted by optical fiber, driver of light emitting diode; digital electronic module for processing spectrum and estimating target analyte concentration; screen for displaying target analyte concentration. The tritium conversion efficiency of the light source can reduce energy waste, so there is almost no waste heat generated. 'This allows the light source to be integrated with the spectrum analyzer. The high-speed signal pulse enables high-sensitivity synchronous measurement technology to be applied because the sortable light The diode has the advantages of speed and elasticity, so measurement and skin temperature control can be performed at the same time. The paper size is subject to the Chinese National Standard (CNS) A4 specification (210X297). (Please read the precautions on the back and fill in this page first.) Order · Printed by the Intellectual Property Bureau of the Ministry of Economic Affairs and Consumer Cooperatives 515706 A7 B7 V. Invention Explanation () Printed by the Intellectual Property Bureau of the Ministry of Economic Affairs, Consumer Cooperatives, Figures, Charts, Charts, Charts, and Diagrams Brief Description 1 is a top view of a standard light-emitting diode, which can emit light at the bottom of the chip 2a and FIG. 2b are respectively a top view and a bottom view of a substrate available for a light-emitting diode according to the present invention; 3 疋 a bottom of FIG. 2 according to the present invention A detailed representation of the object, which is depicted with a parabolic refraction lens on which the light emitting diode is mounted; 4 is a detailed close-up table TF diagram of the substrate / light emitting diode subassembly according to the present invention; 5 is according to the present invention Inventive Figure 4 is a sectional view of the subassembly; 6 is a TSI · group showing a three-dimensional space of a light-emitting diode light source module according to the present invention, and 7 is a three-dimensional view of a printed circuit board (pCB) of a light-emitting diode light source module according to the present invention 8 is an isometric projection view of a miniaturized spectrum analyzer according to the present invention; 9 is a three-dimensional schematic diagram of a photodiode module according to the present invention; 10 is an optical module including a miniaturized spectrophotometer as shown in FIG. 8 The three-dimensional space schematic diagram of the photodiode module of FIG. 9; 1 1 is the three-dimensional space schematic diagram of the non-invasive blood glucose measurement device using the light-emitting diode as the light source according to the present invention; 12 is the photodiode Another alternative form of polar module.囷 # Cross-reference Note Page 10 This paper size is in accordance with Chinese National Standard (CNS) A4 (210X297 mm) (Please read the precautions on the back to write this page) Binding and binding. 515706 A7 B7 V. Invention Description () Economy Printed by the Intellectual Property Bureau Staff Consumer Cooperative 10 Luminescent Diodes 10 Contact Areas 11 Contact Points 13 Luminescent Diode Substrates 20 Body 21 Front of the Substrate 22 Detailed Schematic 24 Back of the Substrate 25 Well 26 Conduit 27 Refractive Lens 30 Subassembly 40 Wire 50 Light source assembly 60 Optical mixer 61 Hollow center 62 Collection fiber 63 Metal ferrule 64 Printed circuit board 65 Thermoelectric cooler 66 Heating tank 67 Hole 73 Spectrum analyzer 80 Entrance slit 8 1 Page 11 Applicable to this paper size China National Standard (CNS) A4 specification (210X297 mm) 515706 A7 B7 V. Description of the invention () Exit slit 82 Linear array detector module 90 (Please read the precautions on the back first to write this page) Detector Array module surface 91 Optical module 100 Instrument 110 Light-emitting diode driver circuit 111 Digital module 112 Liquid crystal display 113 Power supply 114 115 interface 116 samples the photosensitive diode array 121 described in detail using a conventional spectrometer is a wine container tungsten lamp as the light source. When the light source is applied to a portable or mobile device, the light source has some disadvantages. The most obvious is the uneconomical of space and energy. In addition, this kind of light source will generate excess thermal energy, resulting in a longer time to stabilize the light source, and the life of the bulb will be shortened accordingly. Printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs In the first embodiment, the present invention provides a light-emitting diode as a light source and light source assembly for non-invasive optical sampling. Light emitting diode light sources have several advantages, such as: • Compact size; • Light emitting diode light sources can only be measured after selection and combination. Page 12 This paper applies Chinese National Standard (CNS) A4 specifications (210X297 mm) ) 515706 A7 B7 V. Description of the invention () The amount of light in the required wavelength region; • A light conversion efficiency / less heat generation; • Light intensity can be easily adjusted; • Continuous wave or pulse mode is available during operation Choice; and • Long life (> 100000 hours) In some recent applications, super-excitation light-emitting diodes are used as alternatives or notice boards for incandescent light sources, showing that light-emitting diodes are suitable as light sources for spectroscopic instruments. When these applications use super-exciting light-emitting diodes that emit visible light as the light source, it is obvious that after this type of light source is improved in the future, there are applications for light sources with different wavelength ranges. Please read the note on the back side first.

Page Order Printed by the Intellectual Property Bureau of the Ministry of Economic Affairs and Consumer Affairs Co., Ltd. Light-emitting diode (LED) A light-emitting diode is a solid element that can be determined at the time of manufacture. It has a narrow band of peak intensity and spectral width. Depending on the performance requirements, the manufacturing method has different methods such as single junction, heterojunction or super-excitation light. The size of the light-emitting diodes also varies, ranging from 01x01x01 to lxlxO.l mm. The light wavelength band is determined by the material used to make the light emitting diode ', not by the method of making the light emitting diode. Light-emitting diodes emit light with a narrow wavelength band. When a light source with a wider band is needed, it can be obtained by combining several different light-emitting diodes. In a specific embodiment of the present invention, light emitting diodes that can emit different wavelengths and have full width at half-peak (FWHM) or higher are used to form a sub-assembly to provide a light source with a wider spectral band. . Page 13 This paper size is in accordance with Chinese National Standard (CNS) A4 specification (210 × 297 public love) 515706 Α7 Β7 5. Description of the invention () In addition, the size of a single light-emitting diode can be very small, such as 300x300x200 microns, so the light source assembly can Miniaturization, and because of the high conversion efficiency, the thermal energy generated is also small. Figure 1 is a bottom view of a typical light emitting diode. The light junction of the transmitter emits light due to the application of voltage. The metal treatment on the back of the bottom will reflect the light emitted by the junction to improve energy efficiency. The top contact point 13 and the back metal treatment can be used to energize the light emitting diode. Luminescent Diode Ceremony Substrate As mentioned above, to obtain a light source with a broad spectral band, several different forms of light emitting diodes must be combined. A novel substrate is available for light-emitting diode light sources for packaging and current connection. This substrate provides the characteristics that make the light source module in the invention work normally: • mechanical support and miniaturization; • current connection; • beam shaping; • light intensity model shaping; and • thermal stability. In addition, the light-emitting diode substrate can make the connection between the light-emitting diodes highly flexible; for example, only one light-emitting diode can be used alone or the light-emitting diode with a single characteristic can be composed according to the characteristics of the light-emitting diode. Polar body group to increase luminous power or luminous power in a selected wavelength range. Due to the design flexibility provided by the substrate of the light-emitting diode, in other specific embodiments, in addition to the light-emitting diode, other types of light sources can be used. For example, page 14 of this paper applies Chinese National Standard (CNS) A4 specifications (210X297 (Mm) (Please read the precautions on the back _ write this page) r 丨 Package. Printed by the Consumers ’Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs 515706 A7 B7 V. Description of the invention (can monitor and control the condition of the measurement area such as skin temperature or surface A laser diode and / or a detector in a hydrolyzed state is used as a specific embodiment of the present invention. Fig. 2a is a light emitting diode substrate 20 according to the present invention. The main body 21 of the substrate may be any substrate having sufficient Made of materials with high mechanical strength, good thermal conductivity, and can be easily shaped into the desired shape, such as different forms of concrete and plastic resin. On the front 22 of the substrate 20 there is an array of wells for light emitting diodes to be placed on Above, the metallized patterns on both sides and sides of the substrate, which will be described in detail below, can be used as a current conductor between the light-emitting diode and the light-emitting diode driver. Figure 2b shows the substrate On face 25, there is a large well 26 that can accommodate an electric heating cooler, which will be described in detail below. The duct 27 is used to connect with the front of the substrate to provide the required channel for collecting optical fibers. Each well 23 is provided with a parabolic refractive lens 30 so that the light emitting diode can be mounted on it. The light emitted by the light emitting diode 10 is Lambertian (light will be scattered to various angles) and is located on the side and the top The junction area 11 is emitted, so the refractive lens 30 is required to make the light emitted by the light-emitting diode form a low-ambiguity beam. An ideal beam, whether it enters the optical fiber or directly contacts the test sample, should improve the light. Figure 3 also provides a detailed schematic 24 of the metallization pattern. The metallization pattern can be designed so that each light-emitting diode can be individually connected or connected to each other in groups to facilitate repeated use. Then, each light-emitting diode The diodes are connected to the light-emitting diode driver individually or in groups. The overall design of the current connection combination and the current connection combination in each group is based on the application and instrument design. 15 pages of this paper are in accordance with Chinese National Standard (CNS) A4 (210X 297 mm) f Please read the notes on the back to write this page} Binding, binding, printing by the Intellectual Property Bureau of the Ministry of Economic Affairs, Consumer Cooperatives 515706 A7 B7- —— " " ._ 5. The description of the invention () changes according to different purposes. The aforementioned light-emitting diode substrates are still manufactured by traditional methods. (Please read the precautions on the back to write this page) Light-emitting diodes / Substrate subassemblies are shown in FIGS. 4 and 5. Subassemblies 40 are assembled from light emitting diodes and substrates. Each light emitting diode is placed in the center of each well 23 and fixed to a refractive lens 30. The bottom, the preferred fixing material is conductive epoxy. As shown in Figure 5, the light emitting diode 10 is connected and conducted by a wire 50 having a suitable size, preferably a gold material. The size of the wire is determined by the diameter of the contact point between the driver and the top corresponding to the light emitting diode. Decided. The front end of the module is equipped with a light-emitting diode that can communicate with each other so as to be suitable for optical activity. In a specific spectral region emitted by the light-source module 60 using the light-emitting diode as a light source, there is better transmission characteristics. Covered with oxygen resin or polymer resin material (not listed). This material I has two important functions: • Provides mechanical protection for fragile conductive wires; • Adjusts the reflection constant of the substrate medium to a suitable range to reduce Fresnel's losses; Intellectual Property Bureau of the Ministry of Economic Affairs The in-phase number printed by the employee's consumer cooperative is the formula of the discount calculation and the filling of the approximate material is required:

Page 6 IX This paper size is in accordance with Chinese National Standard (CNS) A4 specification (210X297 mm) 515706 A7 B7 V. Description of the invention () nLED = refractive index of light-emitting diode; N, refractive index of substrate material. Light Emitting Diode Light Source Assembly FIG. 6 shows a light source assembly 60 integrated with a light emitting diode / substrate. The light from the light source must be transmitted to the sample interface or directly to the measurement area. This work has traditionally been expensive with a spatial optical arrangement of many mirrors and lenses. However, it is not suitable for applications requiring miniaturization and space saving. In addition, this design is very sensitive to vibration or other disturbances, making it unsuitable for portable instruments. The present invention utilizes an optical mixer 61 to take advantage of its simplicity and miniaturization. The preferred mixer 6 i is a special large-diameter optical fiber, whose diameter is determined by the optimal optical path that can penetrate the skin, and has a hollow center 6 2 to receive and collect the optical fiber 63. The preferred material for the mixer is low hydroxide silicon fiber, but other materials with high reflection constants such as sapphire or silicon can also be used. The mixer can completely mix the different wavelengths of light emitted by the light-emitting diode, and make the light intensity normal distribution, without causing excessive loss of light intensity. While the mixer 61 is covered with resin, it is preferably fixed on the substrate, but it may be fixed to it with a mechanical fixer. The best collection fiber is a low-hydroxide silicon fiber with a center diameter of about 600 microns. As shown in the figure, the collection fiber is a short straight fiber, which is fixed in the hollow part of the center of the mixer 6 2 and increases through all light source components. Light collection efficiency, the collection fiber 62 can be fixed to the mixed page by sticking. Page 17 This paper size applies Chinese National Standard (CNS) A4 specification (210X297 mm) (Please read the precautions on the back to write this page) Printed and ordered by the Intellectual Property Bureau of the Ministry of Economic Affairs, printed by the Consumer Cooperatives 06 5 5 Printed by the Intellectual Property Bureau of the Ministry of Economic Affairs, the Consumer Cooperatives of the Ministry of Economic Affairs This paper is printed in accordance with the Chinese National Standard (CNS) A4 (210 × 297 mm) A7 _________- ------ Description of the invention () House II 61. The bundle end of the collection fiber can be reinforced with a metal surface 64 (ferrule 64) sized to fit the entrance of the spectrum analyzer described below. As shown in FIG. 6, the component includes at least a light emitting diode 10, a substrate 20, a full house 61, and a collection optical fiber 63. The component is placed on a self-aligning notch in front of the printed circuit board 65. FIG. 7 is a printed circuit board Detailed design drawing, its purpose is to provide additional mechanical support for the substrate and to provide a current connection between the drive electronics and other parts of the light source. The function of self-calibration is to use a hole 7 3 that passes through the bottom of the notch on the printed circuit to the rear of the printed circuit board, so that the thermoelectric cooler 66 that puts the cold side in the well 25 behind the substrate 20 can be placed on it. The thermoelectric cooler 66 is used to stabilize the substrate temperature and also has a low degree of cooling effect. Because the efficiency of light-emitting diodes increases significantly at low temperatures. Therefore, the corresponding heating tank 67 is installed on the hot side of the thermoelectric cooler to save energy during operation. Due to space and energy economics, the light source module equipped with a light emitting diode is suitable for a portable analysis device for non-invasive blood analytes, such as glucose. The following describes the miniaturized, light-weight light source module Device for non-invasive measurement of analytes in blood. Apparatus for non-invasive measurement of analytes in blood. The device 11 of FIG. 11 is a device for irradiating a subject's skin sampling position with light having a broad spectrum band. The back-diffused light that penetrates or is reflected by the skin is collected by the grating in the spectrum analyzer and then the light beam is scattered to analyze its spectral information. The scattered spectrum is projected onto the analysis range. Page 18 -------------- '__ (Please read the notes on the back δ, write this page) One. Order. 515706 A7 B7 Five Description of the invention () On a wavelength-sensitive linear array of detectors, the detector records the received signals and converts them into voltages. After the signal is processed and digitized, the signal processor in the digital electronic component module estimates the concentration of the target analyte in the blood based on a prediction algorithm. Optical module As shown in Figure 10, the optical module 100 includes at least a mini-spectrum analyzer 80 and a related linear array detector module 90. Spectral Analyzer Printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs. Figure 1 1 is made in accordance with the present invention and equipped with a spectral analyzer 80 for non-invasive measurement of analytes in blood. 11 ′ The spectral information transmitted from the optical fiber 63 is collected and dispersed using a single light grating (not shown). As shown in Fig. 8, the spectrum analyzer has an entrance slit 81 and an exit slit 82. In order to transmit the light into the detector array having the same length or approximately the same length as the slit, the width of the input slit 8 1 is designed to be a single pixel width of the detector array. The height of the array (for example, 500 μm) determines the size of the collection fiber (for example, 600 #m). The light is emitted from the collection fiber 63 and collected by the entrance slit of the spectrum analyzer 81. The angled mirrors (not shown) and concave single light gratings (not shown) in the spectrum analyzer are made up of traditional arrangement methods known to those skilled in the art. The mirror reflects the light from the collection fiber at a 45-degree angle and directs it to the diffraction grating. Diffraction gratings on the one hand disperse light into individual components of the spectrum and on the other hand enable the light to be focused on the focal plane of the detector array. Spectral Analyzer's Exit Narrow Page 19 This paper size is applicable to China National Standard (CNS) A4 (2l0x 297 mm) 515706 Five A7 B7 Description of Invention () a But the show array is slightly wider to avoid cuts through exports The slit will be designed earlier than the brakes. See you ... I # 礼 佘 声 性. The flattened slit of the scattered spectrum is emitted to the detector array. The part of the positive MA of the knife is formed by a square slit located on the surface of the linear array of the detector array. The total active area is comparable. It should be noted that 'Although this embodiment uses the above-mentioned spectrum analyzer, other configurations can also be used here, as long as it meets the important requirements when designing a portable instrument, such as small size and low scattered light. (Please read the precautions on the back δ and write this page first) r 丨 Installation. Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs. The spectrum of the detector queue module that is replaced with a silicon multiplexed light wavelength array is a linear array of photodiodes (PDA), each of which is optimized to the required light wavelength range , Record it. Schematic diagram of the PDA module 90. The detector type in the PDA module 90 is an art for those familiar with integrated circuit design and electronic packaging. As mentioned earlier, the module is configured in such a way that the flat image of the scattered spectrum is projected onto the mixing surface located behind the detector array module surface 91. Figure 12 provides another PDA module option. The module-shaped array of photodiodes 1 2 1 is known in the art as a focus plane array. The hybrid focusing plane includes a linear array of photodiode arrays and a semiconductor wafer connected by a metallized substrate. The photodiode is made of a material suitable for the area detected by the detector, such as InGaAs, Ge or InAs. After the light diode array of the line measures the light energy, it records and converts the signal, and the voltage intensity represents the detected light intensity. Page 20 This paper applies the Chinese National Standard (CNS) A4 specification (210X297 mm) 515706 A7 B7_ V. Description of the invention () Each photodiode in the photodiode array represents a pixel and will be recorded at the same time Project a point in the spectrum to record the full spectral range. Then use the silicon multiplexer to output the signal. Finally, the measured values are sequentially output through the following signal processing and analog-digital circuits. Better multiplexer chips have the following characteristics: • Low system noise; • Programmable feedback; • Elimination of offset voltage; • High read speed. The optimal scan acquisition speed per second is determined by the amount of light available (integral time) and the readout speed. The present embodiment of the present invention has a read speed range between 100 scans per second and 1,000 scans per second. The linear array detector array module 90 is assembled in a single printed circuit board (PCB) with the aforementioned signal processing circuit and analog-to-digital converter. The traditional circuit has the following characteristics to provide signal processing: • Buffering; • impedance matching; • frequency smoothing; and • level-shifting. After the signal is processed, the signal can be converted to a digital signal by a traditional analog-to-digital converter (ADC). II. Electronic components page 21 This paper size applies Chinese National Standard (CNS) A4 specifications (210X 297 mm >), (Please read the legal notice on the back ^^, write this page) 1 Printed by the Ministry of Intellectual Property Bureau ’s Consumer Cooperative Cooperative 515706 Printed by the Ministry of Economy ’s Intellectual Property Bureau ’s Consumer Cooperative Co-operative printed A7 B7 Description of the light-emitting diode driver Diode 10 power and control its light emission. The better light emitting diode driver provides the following functions: • The ability to address sequentially connected light emitting diode groups / individual light emitting diodes each time; • Each time The ability to address the light emitting diode group / individual light emitting diodes in the light source at the same time; • It can flexibly sort the light emitting diode group / individual light emitting diodes according to the programmed data in the memory; • Adjust the light intensity by the amplitude. In addition, the light emitting diode driver circuit emits a relatively short pulse with a duration of only 100-400 nanoseconds, with a programmable peak current of up to 10 amps In addition, the light-emitting diode driver can also drive the light-emitting diode in a continuous wave mode at a moderate current intensity (100-5 00 mA) or the above-mentioned light-emitting diode in an AC or DC mode. The ability of a polar body driver is well known to those skilled in the art, so the light-emitting diode driver is not drawn in detail here. High-speed digital electronic module High-speed digital electronic module containing a digital signal processor (DSP) 1 1 2. Provide the functions required to process the glucose measurement algorithm, which is recorded in non-volatile memory such as read-only memory or erasable read-only memory. The signal processing functions provided include: Page 22 Paper size applies Chinese National Standard (CNS) A4 specification (210X297 mm) (Please read the notes on the back to write the clothing page) 515706 V. Description of the invention (• High degree-Handling all necessary for accurate blood analyte measurement Sequence and synchronize events; • Low level-Monitor the status of important instrument indicators to get data. Volatile memory is used to store digital data. Measurement status indicators The results will be displayed on the following high-resolution LCD display. Power supply (1 14) Many light weight and high efficiency batteries are suitable for power supply. The preferred choice of this instrument is that the size and weight are both Optimized Ni = metal nickel-metal hydride battery for hand device ^ ............. packing (please read the back of the page first: ¾ Italian matter to write this page) Staff of Intellectual Property Bureau, Ministry of Economic Affairs The consumer cooperative prints the π · control and display This device is controlled by the button panel 115. In other and " embodiments, π language prompts can be used to identify the chip through the spoken language of the digital module 112: without manual control Way to control the instrument. Measurement results and reports are displayed on the high-resolution I crystal display U 3 placed on the top of the instrument for easy reading. 9 As shown in Figure 11 'The miniaturization and compactness of this instrument 110 The light-emitting diode chirped light source is achieved with the unique design used to illuminate the measurement area and collect the light emitted from the measurement area. The light source is located very close to the measurement area, and the distance between the light source and the measurement area is controlled by the mixer. The necessary distance for mixing in the spectrum and the intensity is reached, and the light emitted by the light emitting diode 10 is in contact with the sample interface U6 through the mixer 61. In the preferred embodiment, the sample interface 116 includes the sample area of the living skin. Page 23 This paper size is applicable to the Chinese National Standard (CNS) A4 specification (21〇χ tear male double) 玎 515706 A7 _B7_ V. Description of the invention () Fiber-optic probes for domain contact. The design of the light source assembly and the central position of the collection fiber allows the spectrum analyzer to be placed close to the light source, and because the light source does not generate heat, the light source and the spectrum analyzer can be placed in the same module. Although the present invention is exemplified by several preferred embodiments, those skilled in the art can easily understand other applications of the present invention without departing from the spirit and scope of the present invention. Therefore, the present invention should be limited only by the scope of the following patent applications. (Please read the notes on the back first to write this page): 丨 Packing · Ordering · Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs Page 24

Claims (1)

515706 A8 B8 C8 D8, patent application scope 1. A light source assembly for non-intrusive optical sampling, which contains a light emitting diode that can provide a wide band signal, the light source assembly includes at least: a variety of light emitting diodes; A light-emitting diode substrate provides mechanical support of the light-emitting diode, and the light-emitting diode and the substrate form a light-emitting diode / substrate sub-assembly; and a light-emitting diode that can conduct electricity between the light-emitting diodes. Device; a device that can shape the light emitted by the aforementioned light-emitting diode; a device that can control the intensity profile; a device that can connect the aforementioned signal to the sampling interface; a device that can collect the aforementioned Device for emitting light from a sample; a device for controlling and monitoring the condition of the measurement location. 2. The component as described in item 1 of the scope of patent application, wherein the light emitting diode is any one of a single junction, a heterojunction or a super-excitation light emitting diode. Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs (please read the precautions on the back ^ fill out this page) 3. For the components described in item 1 of the scope of patent application, the light-emitting diodes will emit different wavelength ranges but wavelength There is overlapping light, so a light with a broad spectrum band can be obtained by combining the light including the aforementioned wavelength range. Page 25 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) 515706 AS B8 C8 D8 5. 6. 7. The scope of patents printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs, such as the scope of patent applications The component according to item 1, wherein the substrate is a substrate having a front, a rear and a side. The component according to item 4 of the scope of the patent application, wherein the device capable of shaping the light emitted by the light-emitting diode includes at least a plurality of parabolic refractive lenses. The aforementioned refractive lenses are placed in a well in front of the substrate. The diodes are fixed in the corresponding refractive lenses, so the differences between the beams are minimized. The component described in item 5 of the scope of patent application, wherein the sub-component mentioned is coated with optically active epoxide or polymer resin to provide mechanical protection and reduce Fresnel (s losses), The coating material has suitable transmission characteristics in a wavelength range emitted by the light source component. The component according to item 6 of the patent application, wherein the substrate is made of a ceramic resin or a plastic resin. 8. The component described in item 7 of the scope of the patent application, wherein the refractive index of the coating is: where: nLED = refractive index of the light-emitting diode; page 26 This paper applies Chinese National Standard (CNS) A4 Specifications (210 X 297 mm), patent application scope Nf A8 B8 C8 D8 • Refractive index of substrate material 9. The component described in item 4 of the patent scope, where the call for connection, " signal to sample The interface device includes at least a mixer, and the pan combiner includes at least a hollow large-diameter high-refractive-index optical fiber, so the intensity and the wavelength information of the light emitted by the forward-backed module are completely processed without additional leakage. . 10. The component according to item 9 of the scope of patent application, wherein the mixer is made of one of low hydroxide silicon, sapphire and silicon. u. The component according to item 9 of the patent application, wherein the mixer is fixed in front of the aforementioned substrate. 12. The component according to item 9 of the scope of patent application, wherein the device for collecting light emitted by the sample includes at least a collection optical fiber, the collection optical fiber at least includes passing through the hollow hole I by connecting the front of the substrate and the rear of the substrate The optical fiber is attached to the trench of the well, and the size of the well is a size that can accommodate the temperature adjustment element. Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs. 13. The component described in Item 12 of the scope of the patent application, where the collection fiber made of low-oxygen silicon has a core diameter of about 600 microns. 14. The component as described in item 12 of the scope of the patent application, in which the luminescence is provided on page 27. The paper size is applicable to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) 515706 A8 B8 C8 D8 _________ VI. Patent application The range-conducting device includes at least wires that enable the light-emitting diodes to be interconnected. 15. The component according to item 14. in the scope of the patent application, wherein the device for transmitting the current between the light-emitting diodes further includes a metallization pattern in front of and around the substrate, and the metallization pattern is used as the same as the foregoing. Conduction between wires. i 6. The component according to item 15 of the scope of patent application, wherein the device for conducting between the light-emitting diodes further includes a metallized pattern on a printed circuit board (PCB), and the aforementioned printed circuit board includes at least the front and the rear There is a self-aligning notch in front of the printed circuit board to receive the aforementioned sub-component. The connection between the aforementioned metallized pattern and the current contact point placed on the substrate on the notch can be used as a light-emitting diode driver and a light-emitting diode. Conductive use. 1 7 According to the component described in the scope of patent application No. 丨 6 of which the light-emitting diode can be individually connected to the light-emitting diode driver ° Printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs i. The component described in item 6, wherein the light-emitting diodes can be connected to the light-emitting diode driver in a group manner. 19. The component described in item 16 of the scope of the patent application has a hole in the printed circuit board. When the sub-component is placed on the printed circuit board, the first page 28 L ______---------- This paper size applies China National Standard (CNS) A4 specification (210 x 297 mm) 515706 6. Scope of patent application The substrate groove and the hole on the printed circuit board can be aligned. 20. The component according to item 19 of the scope of patent application, wherein the device for adjusting temperature includes at least a thermoelectric cooler and a heating tank having a cold surface and a hot surface, and the thermoelectric cooler passes through the aforementioned hole and the rear well. So that the cold surface is in contact with the rear of the substrate; and a heating tank is in contact with the hot surface of the thermoelectric cooler; wherein the thermoelectric cooler and the heating tank constitute a sub-assembly. 2 1. The component as described in item 20 of the scope of patent application, further comprising a supporting metal ferrule, wherein the aforementioned collection optical fiber passes through the hole on the aforementioned printed circuit board and the aforementioned thermoelectric cooler and heating tank sub-assembly, It is then combined with a metal ferrule located behind the aforementioned printed circuit board. 2 2 · —A small, lightweight instrument for non-invasive blood analyte measurement, which contains a prediction algorithm that analyzes the light emitted from the sample area by a spectral analysis method to estimate the target analyte concentration. The instrument contains at least: A light source assembly for non-invasive optical sampling. A light emitting diode is incorporated in the assembly to provide a wide signal band, and then the signal band is transmitted to the tissue measurement area and collects the light emitted by the aforementioned sample. An optical module for Disperse, focus, and measure the light emitted by the sample; a light-emitting diode drive for driving the light-emitting diodes on page 29. This paper size applies the Chinese National Standard (CNS) A4 specification (210 X 297 mm> ( (Please read the notes on the back and fill in this page first) r 丨 Installation: Printed by the Employees' Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs 515706 A8 B8 C8 6. A patent application scope; a processor executing the aforementioned prediction algorithm; and an output device for outputting the aforementioned measurement result of the analyte. 23. The apparatus according to item 22 of the scope of patent application, wherein the light source component includes at least: a plurality of light-emitting diodes; a light-emitting diode substrate, which provides mechanical support for the light-emitting diodes, so The aforementioned light-emitting diode and substrate constitute a light-emitting diode / substrate sub-assembly; a device for controlling a light intensity paradigm; a device for providing a conduction current between the aforementioned light-emitting diodes; A device for emitting light from a diode; a device for connecting the aforementioned signal to a sample interface; a device for collecting the emitted light from the sample; a device for controlling and monitoring the tissue sample area. Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs. 24. The device described in item 23 of the patent application scope, in which the light-emitting diodes emit light of different wavelength ranges but the wavelength ranges intersect. Combined to get a single wideband signal. 2 5 · The instrument described in item 23 of the scope of patent application, in which the substrate is on page 30. The paper size is applicable to China National Standard (CNS) A4 (210 X 297 mm) 515706 A8 B8 C8 -—-一 D8 __ VI. The scope of patent application includes front, rear and side. (Please read the precautions on the back first and fill in this page) 2 6. The instrument described in item 25 of the scope of patent application, in which the plastic mold is made of light. The device of the beam emitted by the polar body 'includes at least several parabolic refraction lenses placed in a well in front of the substrate' and the above-mentioned light-emitting diodes are fixed in the corresponding refractive lenses. The device described in item 26 of the scope of the patent application, wherein the sub-component is coated with optically active epoxide or polymer resin to provide mechanical protection and reduce Fresnel's abrasion (Fresnel's 10 sses). The dressing has suitable transmission characteristics in the wavelength range emitted by the light source component. Printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs 28. The instrument described in item 26 of the scope of patent application, The device for transmitting the signal to the sample interface includes at least a mixer 'the mixer includes at least a hollow, large-diameter, high-reflection-coefficient fiber', so the light emitted by the aforementioned sub-component can be completely mixed with the included wavelength information There will be no additional leakage. 2 9. The device described in item 28 of the patent application 'where the mixer is fixed in front of the aforementioned substrate. 3 0. As described in item 29 of the patent application Apparatus, collected from the sample · page 31 This paper size is applicable to China National Standard (CNS) A4 specification (21〇χ 297 mm) 515706 A8 B8 C8 D8 6. Scope of patent application (please read the precautions on the back first_fill in (This page) The light emitting device of the firm includes at least a collection optical fiber. The collection optical fiber includes at least an accessory optical fiber that passes through a hollow hole and passes through a groove connecting a well in front of the substrate and a well behind the substrate. The temperature regulating element is housed. 3 1. The device according to item 27 of the scope of patent application, wherein the device for the current connection between the light emitting diodes is at least Contains wires that can connect the light-emitting diodes to each other. 3 2. The device as described in item 31 of the scope of patent application, wherein the device for transmitting the current between the light-emitting diodes further includes metallized patterns in front of and around the substrate. Here, the metallized pattern is used to conduct electricity with the aforementioned wires. Printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs 3 3. The instrument described in item 32 of the scope of patent application, which is used for light-emitting diodes. The device for energizing further includes a metallized pattern on a printed circuit board. The printed circuit board includes at least the front and the rear. The front of the printed circuit board has a self-aligning notch for receiving the aforementioned sub-components. The connection between the current contact points on the substrate on the mouth can be used for conduction between the light-emitting diode driver and the light-emitting diode; wherein the light-emitting diode is connected to the light-emitting diode driver individually or in groups. 32 pages of this paper are in accordance with Chinese national standard (CNS> A4 size (210 X 297 mm) 515706 A8 B8 C8 D8 VI. Patent application scope (please read the back first) (Please note this page before filling in this page) 3 4, The device described in item 3 of the scope of patent application, where the printed circuit board has a hole 'When the subassembly is placed on the printed circuit board, the aforementioned substrate groove and Holes on the printed circuit board can be aligned. 35. The device according to item 34 of the scope of patent application, wherein the device for adjusting temperature includes at least a thermoelectric cooler and a heating tank with a cold surface and a hot surface, and the thermoelectric cooler passes through the aforementioned hole and the rear Wells are connected, so the aforementioned cold surface is in contact with the rear of the substrate, and a heating tank is in contact with the hot surface of the cooler, wherein the thermoelectric cooler and the heating tank constitute a thermoelectric cooler_heating tank vice Components. 3 6. The instrument as described in item 35 of the scope of the patent application, further includes a supporting metal and a ferrule, wherein the aforementioned collecting optical fiber passes through the hole on the aforementioned printed circuit board and the aforementioned thermoelectric cooler and heating tank sub-assembly. Then, it is combined with the metal hoop located behind the printed circuit board. 37. The instrument described in item 30 of the scope of the patent application, where the optical module contains at least: The Intellectual Property Bureau of the Ministry of Economic Affairs, a consumer cooperative, prints a miniaturized entrance A mini-spectrum analyzer with an exit slit; a linear array of detector array modules' The spectrophotometer and detector array module are fixed to each other, so the aforementioned exit slit and detector array module will be aligned Pair arrangement. Page 33 This paper size is in accordance with China National Standard (CNS) A4 (210 x 297 mm) 515706 A8 B8 C8 __ D8 VI. Patent Application Scope 3 8 · The instrument described in Item 37 of the Patent Application Scope, where The width of the input slit is equal to the width of a single pixel in the linearly arranged detector array in the linearly arranged detector array assembly. 39. The instrument as described in item 38 of the scope of patent application, wherein the spectral analysis The analyzer includes at least: a mirror that reflects and projects the light emitted by the aforementioned collecting fiber into the entrance slit; a parabolic single light grating that disperses and focuses the light reflected by the aforementioned mirror; The flat portion of the spectrum is projected onto the detector array via an exit slit. 40 · The device as described in item 40 of the scope of the patent application, wherein the π detector printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Photographic Economics includes at least one linear array of photodiodes. The polar body contains at least a detector element and a related multiplexer element. Each photodiode represents a pixel. Each photodiode simultaneously records a pixel portion of the projection spectrum to record the full spectral range. The spectral portion is converted into a voltage by the aforementioned detector element. 4 1. The device according to item 40 of the scope of patent application, wherein the multiplexer element reads the signals in a serial manner through the aforementioned detector element. Page 34 This paper size is in accordance with Chinese National Standard (CNS) A4 (210 X 297 mm) 515706 A8 B8 C8 D8 6. Application for patent scope 4 2. The instrument described in item 41 of the scope of patent application, the middle line The array of detector array modules further includes a signal processing circuit, wherein the aforementioned multiplexer component transfers the aforementioned signal to the signal processing circuit and processes it in any of the following ways: buffering; impedance matching; frequency smoothing; and level shifting . 43. The apparatus as described in item 42 of the scope of patent application, wherein the linear array detector array module further includes an analog-digital conversion circuit (ADC), wherein the aforementioned signal processing circuit transmits the processed signal to the ADC; The ADC converts the processed signal into a digital signal and converts the digital value into the aforementioned processor. 44. The apparatus according to item 43 of the scope of patent application, wherein the processor includes at least a high-speed digital electronic module, the module includes: a digital signal processor; at least one memory; wherein the above-mentioned module is received by an optical module The numerical value sent by the group is calculated by a prediction algorithm that can estimate the target analyte concentration. Page 35 This paper size applies Chinese National Standard (CNS) A4 (210 X 297 mm) (Please read the notes on the back and fill in this page first): Binding · Intellectual Property Bureau, Ministry of Economic Affairs, Consumer Consumption Cooperative, Yinchuang Nong 515706 A8 B8 C8 D8 Patent application scope 45. The device described in item 44 of the patent application scope, wherein the memory includes at least non-volatile memory for storing algorithms. 46. The apparatus described in item 44 of the scope of patent application, wherein the memory includes at least volatile memory for storing digital data. 47. The device according to item 31 of the scope of patent application, wherein the light-emitting diode driver includes at least: a module capable of light-emitting diode power, so the aforementioned light-emitting diode-specific light-emitting diode is: Group of light-emitting diodes or individual light-emitting diodes that are addressable each time; Group of light-emitting diodes or individual light-emitting diodes that can be addressed simultaneously each time; wherein the selected light-emitting diode group or selected light-emitting diodes Diodes can be flexibly sorted according to the stylized sorting data in the memory. 0 Please read the precautions on the back Ii · Bu Ting. Completed. Page order printed by the Intellectual Property Bureau Staff Consumer Cooperatives of the Ministry of Economic Affairs 4 8. The instrument described in item 47 of the patent scope, in which the module can generate pulses with a duration of about 100-400 nanoseconds, with a programmable peak current of up to 10 amps, the module can also Drive the light-emitting diode or group of light-emitting diodes in continuous wave mode at a current intensity of about 1 00-5 00 milliamps, of which the driver can be used on page 36. This paper is in accordance with China National Standard (CNS) A4 (210 X 297 mm) 515706 A8 B8 C8 D8 6. Scope of patent application The LED or LED group is driven in AC or DC mode. (Please read the precautions on the back _ fill out this page first) 49 · The instrument described in item 22 of the scope of patent application, where the display includes at least a high-resolution liquid crystal display. 50. The device according to item 22 of the scope of patent application, further including a power supply device. The aforementioned power supply device includes at least a hydrogen recording battery. 51. The device according to item 22 of the scope of patent application, further comprising a button device, wherein the aforementioned control device includes at least a keyboard and a spoken language recognition module. 52. The apparatus as described in item 23 of the scope of patent application, wherein the sample interface includes at least one optical fiber. Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs, page 37 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm)