504371504371
五、發明說明（/ ) 經濟部智慧財產局員工消費合作社印製 發明目的 本發明係關於利用靈芝菌絲體於果膠、果汁或果菜汁 中’尤其是含果膠之果菜汁中，培養所獲得之果菜汁靈芝 液產品，以及其相關之製法。 發明背景 果膠(pectin)為一種存在於所有植物組織之細胞壁中 之多醣體，分子量約為2〇,〇〇〇〜4〇〇,〇〇〇，屬於水溶性的食 物纖維，廣泛地存在於各種蔬果中，在柑橘類果皮中含量 尤其豐富，天然果膠係呈D_聚半乳糖醛酯 (polygalacuronate)之型式，尤以D-聚半乳糖酸曱酯居多， 其間可能穿插一些鼠李糖殘基（L-rhamnose residues ); 一些天然糖如· D-半乳糖（gaiact〇se)、L-阿拉伯糖 (arabinose)、D-木糖(xyiose)、及乙_巖藻糖(foc〇se)會在果 膠分子上形成側鏈。 已知各種蔬果如草莓、香蕉、鳳梨、木瓜、蘋果、柑 橘類水果（如檸檬、橘子、柚子、葡萄柚等）、火龍果、 奇異果、柿子、愛玉'哈密瓜、梨、甘蔗、青豆、乾豆、 紅蘿葡、高麗菜、花椰菜、南瓜、馬鈴薯、蕃薯等含有豐 富的果膠、各種維生素、礦物質、纖維素等營養成分。丑 許多已發表的醫學研究顯示，果膠能保護腸壁，使腸 内的有用菌活性化，調整腸機能；此外，果膠亦可吸收膽 汁酸、膽固醇，預防南血脂症和高血壓。 3 靈芝是一種木質腐生茵，喜高溫多濕及通風良好之環 ----·---：-----,ΦΜ (請先閱讀背面之注意事項本頁) 訂--------- 本紙張尺度適用中國國家標準（CNS)A4規格（210 x 297公 專利申請案第90122954號 ROC Patent Appln. N〇.90122945V. Description of the invention (/) Printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs Purpose of the invention The present invention relates to the use of ganoderma mycelium in pectin, fruit juice or fruit and vegetable juices, especially in fruit and vegetable juices containing pectin. Ganoderma lucidum product obtained from fruit and vegetable juice, and its related manufacturing method. BACKGROUND OF THE INVENTION Pectin is a polysaccharide that exists in the cell walls of all plant tissues. It has a molecular weight of about 20,000 to 40,000, and is a water-soluble dietary fiber. It is widely found in Among various fruits and vegetables, the content is particularly rich in citrus peels. The natural pectin is in the form of D_ polygalacuronate, especially D-polygalactonate, which may be interspersed with some rhamnose residues. (L-rhamnose residues); some natural sugars such as · D-galactose (gaiactose), L-arabinose (arabinose), D-xylose (xyiose), and ethyl fucose (focose) Will form side chains on the pectin molecule. Various fruits and vegetables such as strawberry, banana, pineapple, papaya, apple, citrus fruits (such as lemon, orange, grapefruit, grapefruit, etc.), dragon fruit, kiwi, persimmon, Aiyu 'cantaloupe, pear, sugar cane, green beans, dried Beans, red dill, cabbage, cauliflower, pumpkin, potato, sweet potato, etc. are rich in nutritional ingredients such as pectin, various vitamins, minerals, and cellulose. Ugly Many published medical studies have shown that pectin can protect the intestinal wall, activate useful bacteria in the intestine, and adjust intestinal function. In addition, pectin can absorb bile acids and cholesterol, and prevent dyslipidemia and hypertension. 3 Ganoderma lucidum is a kind of woody saprophytic fungus. It likes high temperature and humidity and well-ventilated ring ---- · ---: -----, ΦΜ (Please read the precautions on the back page) Order ----- ---- This paper size applies to China National Standard (CNS) A4 specification (210 x 297 Public Patent Application No. 90122954 ROC Patent Appln. No. 90122945
Amended Pages of the Chinese Specification - Enel. II (民國91年5月匆曰修正並送呈） (Amended & Submitted on May 开 2002) .赞『參)r五、發明k萌·(i 經濟部智慧財產局員工消費合作社印製 ^，其分類為··擔子菌亞門，無褶菌目，多孔菌科，靈芝 ，。近年來由於人工栽培的研究成果顯示其代謝物中含有 諸夕具商療作用之活性成分，如多醣體（ )、類三萜、有機鍺化合物及腺苷等。如果將國内外靈芝 之研究文獻詳加整理分析，發現靈芝具有抗癌、降血壓、 降血糖、抑制膽固醇、免疫及保肝的生理作用及藥理效果 ，所以靈芝在日本早已大行其道，除醫藥學界對其積極研 究探討外，各大藥廠更競相製造，但是由於栽培成本過高 ，所提供之靈芝產品價位始終居高不下，讓此種優異產品 不夠普及化以廣泛嘉惠大眾之健康。 已知靈芝多醣消滅癌細胞的機制：當靈芝多醣與巨噬 細胞一起培養時，由產生之酵素分解高分子多醣類，產生 小刀子的各種葡萄糖誘導體，而且其會誘導巨嗟細胞分泌 免疫物貝·干擾素(INF)-y、介白素_丨、介白素_2等等， 使T細胞變成殺手T細胞，殺手τ細胞是具有攻擊能力咐 細胞’產生干擾f可刺激免疫細胞變為天然殺手（Natural Killer)細胞’其對0可以抵抗癌細胞，渺卜可以找尋不 屬於體内的生物以攻擊之。 1881年Κ_η根據具有表面漆光和g柄的白腐型真菌 W· Cut)為基礎而建立了靈芝屬（ 如)。$芝在藥理研究和人工栽鱗最常被使用的 學名為G /⑽·—，但常常在其中發現同名異物的情況，事 實上是將-群形__$芝群(G·喊㈣_piex)當作 同一種。在所謂的靈芝群（G /WCW_ c〇_ex)中，其實 91. 3. 2,000 (請先閱讀背面之注意事項再填寫本頁) 訂· 4 504371 本?卜 A7 B7 五、發明說明（3) 包含有松杉靈芝(G· tsugae Murr·)，凡勒斯靈芝(G. valesiacum Boud·),奥勒岡靈芝（g· 〇reg〇nense muit·)，銳斯那靈芝（g. resinaceum Boud·)，菲力靈芝(g· pfeifferi Bres·)，奥斯特靈芝(〇 oerstedii (Fr·) Torr·)，阿瑪延靈芝(g· ahmadii Stay·)，等形態相近的 類緣近屬；已知靈芝屬有四十多種；本文所提及之靈芝係泛指 各種可食用之靈芝。 1998年11月台北榮民總醫院醫學研究部教授王聲遠即發表 一篇「靈芝免疫調節作用與抗腫瘤活性之關係」研究報告。他 指出，使用高純度化、高倍數的靈芝抽取液在人體内，可增強 人體的免疫細胞作用，進而可直接或間接影響抑制到腫瘤。王 教授表示，他將免疫細胞培養在培養液内，發現靈芝對免疫細 胞有增強的作用，而免疫細胞所產生的細胞因子(cyt〇kine)可以 抑制腫瘤的成長。研究顯示，巨噬細胞培養液中的介白素—i (IL-1)、腫瘤壞死因子（TNF)，及介白素一6經過靈芝萃取 液處理後，將比未經處理的對照組分別多出41倍、9·8倍及29 倍，這將可證明靈芝可以刺激Τ淋巴細胞、巨喔細胞產生細胞 因子，抑制腫瘤細胞的生長。 經濟部智慧財產局員工消費合作社印製 鑑於靈芝之前述優點以及各種蔬果之豐富營養成份，本 發明意外地發現利用果膠、果汁或果菜汁，尤其是含果膠之 果汁或果菜汁，培養靈芝之g絲體，所培養出來的菌絲體會 分泌各種分解㈣體的酵素，這些酵素在分解果膠及其他存 在之多體後，產生各種分子較小的<Κ3>4<1_6>醋類（例 如：雙醣類、半纖維素(hemicellulose)以及其他短鏈的多醣 體）的混合物’而發揮抗癌作用；如此竟可培養出與已知 〜5〜 A7 B7 五、發明說明（孕 經濟部智慧財產局員工消費合作社印製 二ίί之保健效果相同之健康食品，甚至可提高靈芝内 有^人體健康之成份含量。 主登盟么技術内交斑特％ 本發明係利用靈芝菌絲體，更明確地說，是利用靈芝 之多體分解酵素，於果膠、果汁或果菜汁中，尤其是含 果f之果汁或果菜汁巾，培養製造出生機食品，由於利用 果菜汁作為培養基以培養靈芝菌絲體時，靈芝苗（菌絲體 叢^得容易生長，遂可得到富含靈芝菌絲體及靈芝苗之生 ，食品，此種產品可提高細胞性免疫力，屬於產業上利用 價值極高之抗癌食品或健康食品。 ^本發明並非以子實體之成份產生為目的，而係利用其 菌絲體所分泌之酵素群將果菜汁中之多醣體成份，特別是 果膠，分解產生抗癌物質。 本發明製法可直接利用果膠或利用一或多種含果膠的 水果或蔬菜以果汁機處理成天然果(菜)汁，視需要，可另 添加一些有利於靈芝生長之輔助劑，在適當稀釋（例如添 加水）並經加熱消毒滅菌後，加入靈芝(例 /wezWww)之菌絲體發酵培養，利用菌絲體所分泌之酵素群 將前述果膠或果菜汁中之多醣體轉變為易消化、吸收且有 抗癌性之具有特殊生物學價值之產物，此等產物可直接調 配分裝製成口服用果菜汁靈芝液，或與習知之食品添加物 ，例如··甜味劑、香料、食用色素等，適當混合後方製成 口服用果菜汁靈芝液；前述產物亦可依習知食品加工方法 {請先閱讀背面之注咅？事項 ---------tr--------- 本紙張尺度適用中國國家標準（CNS)A4規格（210 χ 297公釐） :-^L^ jAmended Pages of the Chinese Specification-Enel. II (Amended & Submitted on May 2002). Amazing [References] r 5. Invention k Meng · (i Intellectual Property of the Ministry of Economic Affairs) Printed by the Bureau ’s Consumer Cooperatives, which is classified as ... Basidiomycotina, Pleuroidea, Poaceae, Ganoderma. In recent years, research results of artificial cultivation show that its metabolites contain Zhu Xi, which has a commercial therapeutic effect. Active ingredients, such as polysaccharides (), triterpenoids, organic germanium compounds, and adenosine. If the research literature of Ganoderma lucidum at home and abroad is carefully analyzed, it is found that Ganoderma lucidum has anti-cancer, blood pressure, blood sugar, cholesterol, The physiological and pharmacological effects of immunity and liver protection, so Ganoderma has long been popular in Japan. In addition to the active research and discussion of it in the medical sciences, major pharmaceutical companies are more competitive in manufacturing, but because the cultivation cost is too high, the price of the provided Ganoderma products is always The high level makes this excellent product not universal enough to widely benefit the health of the public. The mechanism of ganoderma polysaccharides to destroy cancer cells is known as ganoderma polysaccharides and macrophages When cells are cultured together, the produced enzymes decompose high-molecular-weight polysaccharides to produce various glucose-inducing bodies of small knives, and they can induce giant pheasant cells to secrete immune substances shellfish interferon (INF) -y, interleukin_ 丨, Interleukin_2, etc., make T cells become killer T cells, killer τ cells have the ability to attack and command cells to 'interfere with f to stimulate immune cells to become natural killer (Natural Killer) cells', which can fight cancer against 0 Cells can be found by attacking organisms that do not belong to the body. In 1881, K. η established the genus Ganoderma (such as) based on a white rot fungus (W · Cut) with surface paint and g handle. The scientific name of $ 芝 in pharmacological research and artificial planting scales is most commonly used G / ⑽ · —, but often the same name of a foreign body is found, in fact, the -group shape __ $ 芝 群 (G · 叫 ㈣_piex ) As the same kind. In the so-called ganoderma group (G / WCW_ c〇_ex), actually 91. 3. 2,000 (please read the notes on the back before filling out this page) Order · 4 504 371 book A7 B7 V. Description of the invention (3 ) Contains G. tsugae Murr., G. valesiacum Boud, G. regregnense muit, and G. resinaceum Boud. ), G. pfeifferi Bres, G. pfeifferi Bres, Oerstedii (Fr.) Torr, G. ahmadii Stay, etc. There are more than 40 types of ganoderma lucidum; the ganoderma mentioned in this article refers to all kinds of edible ganoderma. In November 1998, Wang Shengyuan, a professor at the Medical Research Department of Taipei Rongmin General Hospital, published a research report on the relationship between Ganoderma lucidum's immunomodulatory effect and antitumor activity. He pointed out that the use of Ganoderma lucidum extract with high purity and high multiples in the human body can enhance the body's immune cell function, which can directly or indirectly affect the suppression of tumors. Professor Wang said that he cultured immune cells in the culture medium and found that Ganoderma lucidum has an enhanced effect on immune cells, and cytokines produced by immune cells can inhibit tumor growth. Studies have shown that interleukin-i (IL-1), tumor necrosis factor (TNF), and interleukin-6 in macrophage culture fluids are significantly less 41 times, 9.8 times, and 29 times more, which will prove that Ganoderma can stimulate T lymphocytes and giant cells to produce cytokines and inhibit tumor cell growth. In view of the aforementioned advantages of ganoderma and the rich nutritional content of various vegetables and fruits, the present invention unexpectedly discovered that the use of pectin, fruit juice or fruit and vegetable juice, especially pectin-containing fruit or vegetable juice, cultivates ganoderma. G mycelium, the cultured mycelium will secrete various enzymes that decompose carcass, these enzymes after decomposing pectin and other existing polysomes, produce a variety of smaller molecules < Κ3 > 4 < 1_6 > vinegars (For example: a mixture of disaccharides, hemicellulose, and other short-chain polysaccharides) to exert anti-cancer effects; in this way, it can be cultivated with the known ~ 5 ~ A7 B7 V. Description of the invention (pregnancy economy The Ministry of Intellectual Property Bureau ’s Consumer Cooperative printed two health foods with the same health effects, which can even increase the content of ^ human health ingredients in Ganoderma lucidum. The present invention uses Ganoderma mycelia More specifically, it is the use of ganoderma lucidum multi-degrading enzymes in pectin, fruit or vegetable juice, especially fruit or fruit and vegetable juice towels. The production of food for the birth machine is because when the fruit and vegetable juice is used as a medium to cultivate the ganoderma lucidum mycelium, the ganoderma lucidum (mycelium plexus is easy to grow, and then the ganoderma lucidum mycelium and ganoderma lucidum seed-rich food can be obtained. The product can improve cellular immunity, and belongs to an anti-cancer food or a health food with extremely high industrial utilization value. ^ The present invention does not aim at producing the components of fruiting bodies, but uses the enzyme group secreted by its mycelium to fruit and vegetable The polysaccharide component in the juice, especially pectin, is decomposed to produce anti-cancer substances. The method of the present invention can directly use pectin or use one or more pectin-containing fruits or vegetables to process the fruit juice into a natural fruit (vegetable) juice. If necessary, additional auxiliary agents that are beneficial to the growth of ganoderma can be added. After appropriate dilution (such as adding water) and heat sterilization and sterilization, add mycelium of ganoderma (eg, wezWww) to ferment and culture. The enzyme group converts the polysaccharides in the aforementioned pectin or fruit and vegetable juice into products with special biological value that are easy to digest, absorb, and have anti-cancer properties. These products can be directly formulated. It is made into ganoderma lucidum juice for oral use, or with conventional food additives, such as sweeteners, spices, food coloring, etc., and is mixed appropriately to make ganoderma lucidum juice for oral use; the aforementioned products can also be made according to conventional foods. Processing method {Please read the note on the back first? Matters --------- tr --------- This paper size applies to China National Standard (CNS) A4 (210 χ 297 mm) :-^ L ^ j
五、發明說明（5 經濟部智慧財產局員工消費合作社印製 進一步調配成各種食品型式， 义 I武如·凝膠、果凍、果醬、洋羹 ^則述錄亦可經冷奸―，縣賴財，戍者再 製成錠舰膠囊奴俾便·或長祕存。這些產品 白土二合生物活性物質(如：半纖維素(hemicellul°se)、活性 α ^(Active Hexose Correlated Compound, AHCC)、靈芝蛋白質、肌醇p3 (⑻姻p3)及/或各種營養素 ’生素之健康食品’此種產品及其製法並未見 诸任何已知技藝。 —·本發明製法之培養條件可採用—般習知之培養操作方式進 仃’例如：先將-或多種含果膠的水果或蔬菜以果汁機處理成 =然果(菜)汁（可直接使用，亦可經過濾處理），置於適當容 器十視f要，可另添加—些有.靈芝生長之辅助劑，如： 葡萄糖或蔗糖等碳水化合物，在適當藩（例如添加五倍量的 水）後，適當加熱以消毒滅菌；冷卻後，添加靈芝菌絲體在 20〜30 C下培養3至14天，宜好氣性培養5至1〇天，培養可於攪 拌或振1下進行’所得產物可直接娜製成口顧果菜汁靈芝 液，或與習知之食品添加物，例如：甜味劑、香料、食用色素 等[適當混合後方製成口服用果菜汁靈芝液；選擇地，亦可將 培養基依習知食品加工方法進一步調配成各種食品型式，如 •凝膠、果束、果醬、洋羹等；選擇地，亦可將培養基全體 以習知凍乾技術乾燥，粉末化或進一步錠劑化或製成膠囊。 此等產品沒有靈芝特有之苦味以及顏色，也沒有毒性，宜於 低溫及/或低渔度下保存。本發明製法之殺菌可採習知方式 進行’例如：加熱、紫外線等。凡熟習此項技術者應可依 1--1 — — — — — —*-------— — — — — — (請先聞讀背面之注意事項再填寫本頁) 本紙張尺度適用中國國冢標準（CNS)A4規格⑽x 297公楚） 91. 3. 2,000 504371V. Description of the invention (5 Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs and further formulated into various food types, Yi Wuru · gel, jelly, jam, pidgin ^ The description can also be raped —— ， County Lai Fortunately, those who have made it can then make tablets of capsules, such as hemicellulose bioactive substances (such as hemicellulse, active αx (Active Hexose Correlated Compound, AHCC)). , Ganoderma lucidum protein, inositol p3 (piont p3) and / or various nutrients 'bio-health food' and its preparation method has not been seen in any known technology.-· The culture conditions of the production method of the present invention can be used- The general practice of the cultivation method is as follows: For example, firstly,-or a variety of pectin-containing fruits or vegetables are processed into a fruit juice (vegetable) juice by a juice machine (can be used directly or filtered), and placed in an appropriate Depending on the requirements of the container, you can add additional supplements for the growth of ganoderma, such as: carbohydrates such as glucose or sucrose, after appropriate heating (for example, adding five times the amount of water), heat appropriately to disinfect; sterilize , Add Ganoderma The silk body is cultured at 20 ~ 30 C for 3 to 14 days, preferably aerobically for 5 to 10 days. The culture can be carried out under stirring or shaking for 1 '. The obtained product can be directly made into mouth-gut fruit and vegetable ganoderma solution, or with Conventional food additives, such as: sweeteners, spices, food colors, etc. [Properly mixed to make oral fruit and vegetable juice and ganoderma solution; Alternatively, the culture medium can be further formulated into various food types according to conventional food processing methods. Such as: • gel, fruit bunch, jam, prickly pear, etc .; optionally, the entire culture medium can be dried by conventional freeze-drying technology, powdered or further tableted or made into capsules. These products do not have the unique bitterness of ganoderma. And the color is not toxic, and it is suitable to be stored under low temperature and / or low fishery degree. The sterilization of the method of the present invention can be performed in a conventional manner, such as heating, ultraviolet rays, etc. Those who are familiar with this technology should follow 1- 1 — — — — — — * -------— — — — — (Please read the notes on the reverse side before filling out this page) This paper size is applicable to China National Tomb Standard (CNS) A4 specification ⑽ x 297 Gongchu) 91. 3. 2,000 504371
五、發明說明（6 ) 經濟部智慧財產局員工消費合作社印製 其經驗選擇最適當之培養溫度、溼度及時間。 各種可食用之靈芝皆可應用於本發明製法中，甚至亦可 利用各種可食類，如：衫、洋益、軸等等，依上述 模式生產出生物活性物質含量不同之生機食品。 各種蔬菜、水果，甚至是含果膠之果皮，如：柑橘類果 皮’皆可應用於本發明製法中，其中以富含果膠之蔬果為較 佳，若採經分離之果膠亦可；視需要，可添加一些可食用輔 助劑以促進靈芝之生長與繁殖及/或改善食用風味。 根據本發明製法製得之產品，經離子交換層析法分析， 證明確實含有下列成份之混合物： 1.靈芝蛋白質； 2·半纖維素(hemicellulose)之各種分解產物； 3. 活性六碳醣相關之化合物（Active Hex〇se c〇rrekted Compound, AHCC)； 4. 肌醇P3(inositol P3); 5. 各種營養素·維生素、植酸(phytic acj^)、礦物質； 6. 酵素：多醣類分解酵素、蛋白分解酵素。 這些成份在已知技藝中業經證明對人體免疫力之增強有莫大 的助益，因此本案能利用果膠、各種水果、蔬果以及其廢棄 物（如·果皮）製造出尚生物價值之高級生化、高技術性食 品，以提高農作物之經濟價值，甚至可適時地解決農產品生 產過剩之問，貫乃兼具進步性及產業利用性之新發明。 〜8〜 (請先閱讀背面之注意事項再填寫本頁) 訂： 適 度 尺 張 紙 本 公 97 2 X 10 2 /V 格 規 4 A S) N (C 準 標 家 90* 2,〇〇〇 504371 Λ7 Β7 經 濟 部 智 慧 財 產 局 員 工 消 費 合 作 社 印 製 五、發明說明（7 實例部分 以下實例旨在進一步說明本發明之技術内容，而非 限制本發明之申請專利範圍。 實例1 將（50克）柚子皮加五倍水蒸煮2〇分鐘後以果汁機攪碎， 過慮，置入二角瓶中，添加1〇倍量的水，於下加熱 約20分鐘以滅菌消毒，冷卻後，添加5%靈芝菌絲體（ /w(:也m)，在室溫及通風良好之情況下振盪 培養七天（培養期間過長時，會產生苦味），使培養液中 產生多量菌絲體。 此培養液可錢食用；若需要贿，則宜於乾燥及低溫條 件下進行。 ' 可《實例1之步驟’以馬鈴薯、蕃薯、南瓜或其他蔬果 之混合物取代柚子皮，亦可麟錄活性㈣含量不 生機食品。 實例2 將（100克）Μ果以果汁機授碎，置入三角瓶中，添加五 倍量的水，於1HTC下加熱約2〇分鐘以滅菌消毒冷 ’添加5%靈芝菌絲體（⑽/__ )，在室、、 通風良好之情況下«培養七天（培養期間過長時^ 生苦味），使培養液中產生多量菌絲體。 曰 本紙張尺度_巾^鮮χ 297公髮 n ϋ 1_1 *1>· ϋ .1 ϋ n H · n ϋν n n ϋ ϋ n 一δ、I I n n n n n n I . (請先閱讀背面之注音？事寫本頁) · -9〜 504371 A7 B7 五、發明說明（没） 此培養液可直接食用；若需要保存，則宜於乾燥及低溫條 件下進行。 (請先閱讀背面之注意事項^^寫本頁) 可重覆實例2之步驟，以香蕉、草莓、鳳梨、木瓜或各種 蔬果之混合物取代蘋果，亦可獲得生物活性物質含量不同 之生機食品。 實例3 小白鼠體内（in vivo )試驗 试驗方法出處·中華癌醫會諸(j· Chinese Oncol. Soc.) 10(3)，14-23，1994 ;中華癌醫會諸(j. Chinese Oncol. Soc.) 11(3)，27-34, 1995 〇 以實例1製得之產品餵食經S-l 80肉瘤細胞接種之純種近親 父配的小白鼠（inbred mice)(每隻體重約30克），每隻 每天餵0.5毫升，30天後檢測S-180肉瘤細胞發展情形；在 與控制組比較下，發現對S-180網狀細胞肉瘤(sarcoma)生 長抑制作用呈陽性反應。 實例4 以實例1製得之產品餵食人類志願者（30位來自抗癌人協 會之經癌症腫瘤切除手術之“過來人”），每人每天服用30 宅升’平均2〜5年内，未見癌症復發。 實例5 經濟部智慧財產局員工消費合作社印製 适麓外(in vitro)試驗 在含實例1或2製得之產品之培養基中培養腫瘤細胞，結果 如下表所示。 10〜 本紙張尺度適用中國國家標準（CNS)A4規格（210 x 297公釐） 504371 A7 _B7五、發明說明（7 ) 1 cc中細/ 包數目 對照組 試驗組 對Hep G2細胞生長抑制 125 100 U-937細胞生長抑制 200 150 腫瘤壞死因子（TNF-α)產生之促進 + +++ 巨噬細胞干擾素（INF-r )產生之促進 + ++ +:表陽性反應 實例6 對S-180肉瘤細胞發生之預防 試驗方法出處··中華癌醫會諸(J· Chinese Oncol. Soc.) 10(3)，14-23，1994 ;中華癌醫會諸(J. Chinese Oncol. Soc·) 11(3)，27-34, 1995。 以實例1製得之產品餵食純種近親交配的小白鼠（inbred mice)(每隻體重約30克），每隻每天银0·5毫升，歷經 一週後，經皮下注射S-180肉瘤細胞並繼續服用實例1製得 之產品；一個月内未發現S-180肉瘤細胞存在。 (請先閱讀背面之注意事 裝--- 寫本頁} t 經濟部智慧財產局員工消費合作社印製 〜11〜 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公釐）V. Description of the invention (6) Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs. Select the most suitable cultivation temperature, humidity and time according to their experience. All kinds of edible ganoderma can be used in the production method of the present invention, and even various kinds of edible products, such as shirts, foreign benefits, shafts, etc. can be used to produce bio-foods with different bioactive substances according to the above model. Various vegetables, fruits, and even pectin-containing peels, such as: citrus peels, can be used in the method of the present invention. Among them, fruits and vegetables rich in pectin are preferred, and the separated pectin can also be used. As needed, some edible adjuvants can be added to promote the growth and reproduction of Ganoderma and / or improve the flavor of edible. The product prepared according to the method of the present invention is analyzed by ion exchange chromatography to prove that it does contain a mixture of the following components: 1. Ganoderma protein; 2. various decomposition products of hemicellulose; 3. active six carbon sugar related Active Hex〇se c〇rrekted Compound (AHCC); 4. Inositol P3; 5. Various nutrients, vitamins, phytic acid (phytic acj ^), minerals; 6. Enzymes: Polysaccharides Decomposing enzyme, proteolytic enzyme. These ingredients have proven to be of great help to enhance human immunity in known techniques, so this case can use pectin, various fruits, vegetables and fruits, and their wastes (such as peel) to create advanced biochemical, biological, High-tech food to improve the economic value of crops, and even to solve the problem of overproduction of agricultural products in a timely manner, is always a new invention with both progress and industrial utility. ～ 8 ～ (Please read the precautions on the back before filling in this page) Order: Moderate rule paper on paper 97 2 X 10 2 / V Grid gauge 4 AS) N (C Standard bidder 90 * 2,00〇504504371 Λ7 Β7 Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs V. Invention Description (7 Examples Section The following examples are intended to further illustrate the technical content of the present invention, but not to limit the scope of the patent application for the present invention. Example 1 Will (50 grams) grapefruit Peel five times water and cook for 20 minutes. Crush with a juicer. After that, put it in a two-corner bottle, add 10 times the amount of water, and heat for about 20 minutes to sterilize. After cooling, add 5% Ganoderma Mycelium (/ w (: also m), shake culture for seven days at room temperature and well-ventilated (when the culture period is too long, bitter taste), so that a large amount of mycelium is produced in the culture solution. This culture solution can If you need a bribe, it should be carried out under dry and low temperature conditions. 'Steps in Example 1' can be used to replace grapefruit skin with potatoes, sweet potatoes, pumpkins or other fruits and vegetables, and the active radon content can also be lost. Food. 2 Divide (100 g) M fruit into a triangle bottle, add five times the amount of water, and heat at 1HTC for about 20 minutes to sterilize and sterilize. Add 5% Ganoderma mycelium (⑽ / __), in a well-ventilated room, «Cultivate for seven days (the culture period is too long ^ bitter taste), so that a large number of mycelia are produced in the culture solution. Paper size _ 巾 ^ 鲜 χ 297 公 发 n ϋ 1_1 * 1 > · ϋ .1 ϋ n H · n ϋν nn ϋ ϋ n -δ, II nnnnnn I. (Please read the note on the back? Write this page first) · -9 ~ 504371 A7 B7 V. Description of the invention ( No) This culture solution can be eaten directly; if it needs to be stored, it should be carried out under dry and low temperature conditions. (Please read the precautions on the back ^^ write this page) Repeat the steps of Example 2 with banana, strawberry, Bromeliad, papaya or a mixture of various fruits and vegetables instead of apples can also be used to obtain bio-foods with different bioactive substances. Example 3 In vivo test method of mice Source: Chinese Cancer Society of China (J. Chinese Oncol. Soc.) 10 (3), 14-23, 1994; j. Chinese Oncol. S oc.) 11 (3), 27-34, 1995 〇 The product obtained in Example 1 was fed to inbred mice (approximately 30 grams each) inoculated with sl 80 sarcoma cells. Each one was fed with 0.5 ml per day, and the development of S-180 sarcoma cells was detected after 30 days. Compared with the control group, it was found that it had a positive response to the growth inhibition of S-180 sarcoma. Example 4 The product prepared in Example 1 was used to feed human volunteers (30 "come people" who had undergone cancer resection surgery from the Association of Anticancer People). Each person took 30 liters per day. 'On average 2 to 5 years, no cancer was seen. relapse. Example 5 Printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs. In vitro test. Tumor cells were cultured in the medium containing the product prepared in Example 1 or 2. The results are shown in the table below. 10 ~ This paper size applies the Chinese National Standard (CNS) A4 specification (210 x 297 mm) 504371 A7 _B7 V. Description of the invention (7) 1 cc medium fine / pack number Control group test group inhibits Hep G2 cell growth 125 100 U-937 cell growth inhibition 200 150 Promotion of tumor necrosis factor (TNF-α) production + +++ Promotion of macrophage interferon (INF-r) production +++ +: Table positive reaction example 6 for S-180 Source of the test method for the prevention of sarcoma cell development ·· Chinese Oncol. Soc. 10 (3), 14-23, 1994; J. Chinese Oncol. Soc · 11 (3), 27-34, 1995. Inbred mice (approximately 30 g each) were fed with the products prepared in Example 1 with 0.5 ml of silver per day. After one week, S-180 sarcoma cells were injected subcutaneously and Continue to take the product made in Example 1; no S-180 sarcoma cells were found within one month. (Please read the note on the back page first --- Write this page} t Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs ~ 11 ~ This paper size applies to China National Standard (CNS) A4 (210 X 297 mm)