499563499563
: --------1--------- (請先閱讀背面之注意事項再填寫本頁) A7 B7 五、發明說明（丨） 【本發明之領域】 本發明係關於一種調製光源的方法及裝置，尤指一種 適用於量測各種微粒子流速的作業。 【本發明之背景】 粒子影像流速儀（P a r t i c 1 e I m a g e: -------- 1 --------- (Please read the notes on the back before filling this page) A7 B7 V. Description of the invention (丨) [Field of the invention] The present invention is The invention relates to a method and device for modulating a light source, in particular to an operation suitable for measuring the flow velocity of various particles. [Background of the Invention] Particle Image Velocimeter (P a r t i c 1 e I m a g e
Vel〇cimetry，piv)被視為未來最具潛力的流速量測裝置 之一，且已被許多製造商及使用者視為流場之重要檢測裝 備’並時常與雷射都卜勒流速儀搭配運用。然而實際上當 該量測裝置應用到微小尺寸流場量測或生醫量測的領域 時，傳統的脈衝光源顯得龐大而累贅，甚至在某些方面可 能對活體樣本造成傷害；此外複雜的維護成本與高單價也 都造成對使用者運作時的負荷，因此在運用上便漸漸有一 =取代的方法出現以提供使用者選用。例如：傳統量測的 情況下，光源必需經由擴展後散成光頁以量測面流場，所 以欲使用連續光使甩時，大部分設計採用旋轉面鏡調制以 模擬脈衝光源。 一就目前應用在微流量測上，一般傳統的方法是利用二 台高功率脈衝雷射，經由鏡組重疊其行走之光路，最後^ = 器形成所需照明光源，兩者之間的時間間隔則 ==步裝置控制。然而其缺點在於所需 積大、費用高及校準難度高。因此傳統的高二二 在使用上有其不便與缺失。 丨脈衝运射 本紙張尺度咖 A7 A7 經濟部智慧財產局員工消費合作社印製 B7 五、發明說明（> ) 發明人爰因於此，本於積極發明之精神，亟思一種可 以解決上述問題之「利用連續光源觀測微粒子流現象之方 法及其裝置」，幾經研究實驗終至完成此項嘉惠世人之發 明。 " 【本發明之概述】 一本發明之主要目的係在提供一種以連續光源模擬脈衝 光源的方法與裝置，俾能以低功率二極體雷射的來產生連 續光源以降低成本並避免對光學元件及樣本的傷害。 本發明之另一目的係在提供一種光源遮斷器與雙反面 鏡之微調設計，俾能將連續光源遮斷以產生模擬之脈衝雷 射，並降低光路校準難度。 本發明之又一目的係在提供一光擴展器，用以調整出 射光為收敛、平行亦或發散。 “為達成上述之目的，本發明一種利用連續光源觀測微 粒子流現象之方法及其裝置，其裝置包括有一連續光源產 ^态，用以產生連續光源；一光源遮斷器，用以遮斷該連 續光源；以及一光學導引器，用以將該連續光源導引至微 子流及一影像擷取器。而實現其方法之步騾為（A)、對 準-連續光源於-光學導引器；（B)、使用—光源遮斷 器連續二次遮斷該連續光源；以及（c)、擷取所得之影 像並送入影像處理器。 心 由於本發明構造新穎，能提供產業上利用，且確有増 進功效，故依法申請發明專利。 日 ‘紙張尺度適用中國國家標準（CNS)A4規格⑽χ 297公爱） I --------------------— (請先閱讀背面之注意事項再填寫本頁) 499563 A7 B7 五、發明說明（3 【圖式簡單説明】 第1圖係本發明之模擬脈衝光源結構圖。 第2圖係本發明_微粒子流速裝置之示意圖。 第3圖係本發明觀測微粒子流速之流程圖。 【圖號説明] 10 二極體雷射 11 13 第二反射鏡 14 20 顯微鏡 2 1 23 個人電腦 24 電子快門 準直鏡 三維平台 影像擷取設備 第一反射鏡 光束擴展器 同步裝置 【較佳具體實施例之詳細説明】 本發明利用連气光源觀測微粒子流現象之方法及其裝 置，主要是為了架設觀測微粒子流速之裝置。該裝置係以 固定流量驅動摻雜粒子之流體於管道内流動，定義光源開 啓與重複頻率，然後啓動同步裝置送出控制訊號到光源調 製開關與影像擷取設備，並經由顯微鏡聚焦後，觀察粒子 影像品質。在狀況不良的情形下，即無法分辨個別粒子， 則由濃度、光源與時間三方面從新調整；若為良好，則將 擷取圖片輸入處理軟體分析，最後分析結果會再經由二次 的判定，也就是根據物理現象或相同條件下多組交叉比 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公釐） 499563 經濟部智慧財產局員工消費合作社印製 A7 五、發明說明（斗） 對，若不合理，則根據軟硬體問題從新操作，反之得到正 確結果’結束量測步驟。 ^ 請參考第1圖所示，第1圖係本發明之模擬脈衝光源 …構圖居模擬脈衝光源結構包括有用以產生連續光源的 桎te：田射1 〇 ,用以遮斷連續光源以產生模擬脈衝光源的 電子快門11 ;第一反射鏡12及第二反射鏡13，第一反射 ’竞用以改夂光束行進方向，並做第一次的光路微調，使 其奴入射之光學元件同高度，且位在光徑正中央，而第二 反射鏡13用以做第二次光路微調，使其保持水平與正中央 入射，用以擴展入射光徑之光束擴展器1 5，並利用調整準 直鏡14的位置改變出射光為收斂、平行或發散。 明參考第2圖所示，第2圖係本發明觀測微粒子流速裝 置之不思圖。本裝置使用一台個人電腦2 3作為主控裝置以 控制影像擷取設備2 4、同步裝置2 2以及量測結果運算； :顯微鏡20充當光學導引器，使得模擬之脈衝光源入射後 經雙色分光鏡反射到物鏡内聚焦，三維平台2丨則負責將樣 本凋整到焦點上，而粒子散射光再循原路徑通過分光鏡與 濾光鏡，由凸透鏡聚焦到針孔上，最後由影像平面擷取马 而在光源訊號的控制方面，總共有四種訊號顯示在示 波器螢幕上，分別由同步裝置22與電子快門u產生。頻 迴1產生控制原始時間間隔的連續觸發訊號，其最高頻率 不得高於快門之設計頻率，而光閘開啓時間則控制曝光長 短，頻逭2負責管制每次快門的作用時段，其設計剛好為 _ 7 私紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公釐） ^ ----1111 III--- (請先閲讀背面之注意事項再填寫本頁} 經濟部智慧財產局員工消費合作社印製 A7 五、發明說明（< ) 人觸發，凡號可通過的時間，因此便可以模擬雙脈衝雷 頻道4則顯示頻道1與頻道2的調制結果；頻道3負責 弟'^光關啓並同時觸發影㈣取裝置24。 、、所示’第3圖係本發明觀測微粒子流速之 况程圖。 步银3 0 1 ·連續光源調制安裝； 步騾3 02 ·•將顯微鏡聚焦到量測平面； 步騾3 0 3 :設定同步裝置，· 步驟304 :擷取影像； 步驟3 0 5 ·掏取影像資料計算；以及 步騾306 :畫出流場向量圖。 综上所陳，本發明無論就目的、手段及功效，在在均 顯不其迥異於習知技術之特徵，為「利用連續光源觀測微 粒子流現象之方法及其裝置」之一大突破，懇請貴審查 委^明察，早曰賜准專利，俾嘉惠社會，實感德便。惟應 注意的是，上述諸多實施例僅係為了便於説明而舉例而 已，本發明所主張之權利範圍自應以申請專利範圍所述為 準，而非僅限於上述實施例。 ' 8 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公釐） r-------^--------- (請先閱讀背面之注意事項再填寫本頁)Velocimetry (piv) is regarded as one of the most potential flow velocity measurement devices in the future, and has been regarded by many manufacturers and users as an important detection equipment for the flow field. It is often used with laser Doppler flowmeters. use. However, when the measurement device is applied to the field of micro-scale flow field measurement or biomedical measurement, the traditional pulsed light source appears to be large and cumbersome, and may even cause harm to living samples in some aspects; in addition, complicated maintenance costs And the high unit price also causes a load on the user's operation, so gradually there is a = replacement method in use to provide users with choices. For example, in the case of traditional measurement, the light source must be expanded into a light sheet to measure the surface flow field. Therefore, when continuous light is used to shake, most designs use rotating mirror modulation to simulate a pulsed light source. As far as the current application is used in micro-flow measurement, the general traditional method is to use two high-power pulse lasers to overlap the walking light path through the mirror group. Finally, the device forms the required lighting source, and the time interval between the two. Then == step device control. However, the disadvantages are the large required volume, high cost and difficult calibration. Therefore, the use of traditional high school sophomores has its inconvenience and deficiency.丨 Pulse transport this paper standard coffee A7 A7 Printed by the Consumers ’Cooperative of the Intellectual Property Bureau of the Ministry of Economics B7 V. Invention Description (>) Because of this, in the spirit of active invention, we urgently think of a way to solve the above problems The "method and device for observing the phenomenon of particle flow using a continuous light source", after several research experiments, has finally completed this invention that benefits the world. [Summary of the present invention] A main object of the present invention is to provide a method and a device for simulating a pulsed light source with a continuous light source, which can generate a continuous light source with a low power diode laser to reduce costs and avoid damage to the light source. Damage to optical components and samples. Another object of the present invention is to provide a fine adjustment design of a light source interrupter and a double mirror, which can interrupt a continuous light source to generate a simulated pulse laser and reduce the difficulty of optical path calibration. Another object of the present invention is to provide an optical expander for adjusting the emitted light to be convergent, parallel, or divergent. "In order to achieve the above-mentioned object, the present invention provides a method and a device for observing a particle flow phenomenon by using a continuous light source. The device includes a continuous light source production state for generating a continuous light source, and a light source interrupter for interrupting the light source. A continuous light source; and an optical guide for guiding the continuous light source to a micro-fluid and an image capturer, and the steps of realizing the method are (A), aligning-continuous light source on-optical guide (B), use—the light source interrupter interrupts the continuous light source for two times in a row; and (c), captures the obtained image and sends it to the image processor. Because the invention has a novel structure, it can provide industry Utilization, and indeed has advancement effect, so apply for an invention patent in accordance with the law. Japan 'paper size applies Chinese National Standard (CNS) A4 specifications ⑽χ 297 public love) I ----------------- ----- (Please read the notes on the back before filling this page) 499563 A7 B7 V. Description of the invention (3 [Schematic description] Figure 1 is the structure diagram of the simulated pulse light source of the present invention. Figure 2 is the book Invention_Schematic diagram of particle flow device. Figure 3 is the present invention Flow chart for observing the flow rate of particles. [Illustration of the drawing number] 10 Diode laser 11 13 Second reflector 14 20 Microscope 2 1 23 Personal computer 24 Electronic shutter collimator 3D platform image capture equipment First reflector beam Expander synchronization device [Detailed description of the preferred embodiment] The method and device for observing the particle flow phenomenon using a connected light source in the present invention are mainly for setting up a device for observing the particle flow rate. The device drives doped particles with a fixed flow rate. The fluid flows in the pipeline, defines the light source on and repetition frequency, and then starts the synchronization device to send the control signal to the light source modulation switch and image capture device, and after focusing through the microscope, observes the particle image quality. In the case of poor conditions, that is, If individual particles cannot be distinguished, the three aspects of concentration, light source and time are readjusted; if it is good, the captured image is input into the processing software for analysis, and the final analysis result will be subject to secondary judgment, that is, based on physical phenomena or the same conditions The following multiple sets of cross-ratio paper standards are applicable to Chinese national standards CNS) A4 specification (210 X 297 mm) 499563 Printed A7 by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs 5. Description of the invention (fighting) Yes, if it is not reasonable, then re-operate according to the hardware and software issues, and otherwise get the correct result ' End the measurement step. ^ Please refer to Figure 1, which is the simulated pulse light source of the present invention ... The composition of the simulated pulse light source structure includes 桎 te: Tian She 1 〇, which is used to block Continuous light source to generate an electronic shutter 11 that simulates a pulsed light source; a first reflection mirror 12 and a second reflection mirror 13; the first reflection is used to change the direction of the beam, and to make the first optical path fine adjustment to make it incident The optical elements are at the same height and are located in the center of the optical path. The second reflector 13 is used for the second optical path fine adjustment to keep the horizontal and center incident. The beam expander is used to expand the incident optical path. , And adjust the position of the collimator lens 14 to change the emitted light to be convergent, parallel, or divergent. Reference is made to Fig. 2, which is a schematic diagram of the device for observing the flow rate of fine particles of the present invention. This device uses a personal computer 23 as the main control device to control the image capture device 24, the synchronization device 22, and the calculation of the measurement results; the microscope 20 acts as an optical guide, so that the simulated pulse light source passes through the two-color after incident The spectroscope is reflected to the objective lens for focusing. The three-dimensional platform 2 丨 is responsible for grading the sample to the focus, and the scattered light of the particles passes the spectroscope and filter along the original path, and is focused by the convex lens onto the pinhole. When capturing horses and controlling the light source signal, a total of four signals are displayed on the oscilloscope screen, which are generated by the synchronization device 22 and the electronic shutter u, respectively. Frequency return 1 generates continuous triggering signals that control the original time interval. Its highest frequency must not be higher than the designed frequency of the shutter, and the shutter opening time controls the exposure length. Frequency 逭 2 is responsible for controlling the time period of each shutter. Its design is just _ 7 Private paper size applies to China National Standard (CNS) A4 (210 X 297 mm) ^ ---- 1111 III --- (Please read the precautions on the back before filling out this page} Employees, Bureau of Intellectual Property, Ministry of Economic Affairs A7 printed by the consumer cooperative. V. Invention description (&); The time when the number can pass, so you can simulate the double-pulse thunder. Channel 4 will show the modulation results of channel 1 and channel 2. Channel 3 is responsible for the communication. Close the Kai and trigger the video capture device 24 at the same time. The third picture shown is the observation of the particle flow rate of the present invention. Step silver 3 0 1 · Continuous light source modulation installation; Step 3 02 · • Focus the microscope Go to the measurement plane; Step 303: Set the synchronization device, Step 304: Capture the image; Step 305 Calculate the image data; and Step 306: Draw the vector diagram of the flow field. The present invention , Methods and effects, all of which are quite different from the characteristics of the conventional technology. It is a major breakthrough in "method and device for observing the particle flow phenomenon using continuous light sources". Patents are a good way to benefit the society, but it should be noted that many of the above embodiments are just examples for convenience of explanation. The scope of the rights claimed in the present invention should be based on the scope of the patent application, not the Only for the above examples. '8 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) r ------- ^ --------- (Please read the back first (Notes for filling in this page)