445374 Π： 發明說明（ A7 B7 (一) 發明技術領域： (請先閱讀背面之注意事項再填寫本頁) 本發明係有關於拋棄式反應模組，尤指一種微量流體 之反應模組，藉由一反應室與複數個試劑室之設置且可控 制其間之流通關係，達成高安全性、方便性、以及機動性 之檢驗工具。 (二) 發明技術背景： 就目前有關運用一般化學/生化之檢驗工具而言，當 使用者欲執行田野(field)水質的測試時，實驗人員必需將所 需之不同試劑、量杯、試管、滴管等器材攜帶至野外。惟 此等實驗器材通常皆儲存於易碎容器中，或由易碎材質所 製成，故致搬運不便。並且在操作危險試劑時，亦可能因 疏忽或不熟悉操作程序，沾染試劑，具有其潛在之危險性， 對於實驗人員之人身安全方面影響甚巨。且當完成所欲測 試的反應物及試劑混合物之處理測試之後，亦有可能對環 境產生污染。是以本發明將針對上述之缺失加以改善，進 而提出一拋棄式反應模組，俾利於實驗人員操作使用。 (三) 發明之簡要說明： 經濟部智慧財產局員工消費合作社印製 本發明之主要目的在於提供一種拋棄式反應模組，尤 其是有關於一種微量流體之反應模組，其中藉由將試劑封 裝於一整個模組內，僅於需要時釋放試劑與反應物相混合 之方式，提供一種具有高安全性、方便性、及機動性的化 學/生化檢驗工具。 本發明之另一目的在於提出一拋棄式反應模組，係可 方便實驗人員將其攜帶至野外以進行預定之試驗。 ΐ紙張尺度適用中國國家標準（CNS)A4規格（210 X ^7公釐） 經濟部智慧財產局BT工消費合作社印製 5314 Α7 ____Β7_____ 五、發明說明（>) 本發明之又一目的在於使用本發明之拋棄式反應模組 時，可去除計量試劑的步驟，大量減少撿驗之時間。 本發明之再一目的在於提供一種製造該拋棄式反應模 組之方法，可依實驗人員不同之需要，將預定實驗所需之 藥劑封裝置該反應模組內。 本發明之另一目的在於提出一拋棄式反應模組，係可 供使用者預先依實驗所需之試劑及實驗步驟封裝試驗及反 應模組。 本發明之又一目的在於提出一拋棄式反應模組，係將 該拋棄式反應模組之尺寸縮小化，便於實驗人員攜帶。 本發明之再一目的在於提出一拋棄式反應模組，係以 一體射出成型之方法製成該拋棄式Μ應模組，具有成本低 廉且易組裝等優點，改善習用技術攜帶、操作不變之缺失。 本發明之次一目的在於提出一拋棄式反應模組，係可 於完成實驗後，可將反應模組拋棄。所以自實驗開始至實 驗結束，實驗入員無需與危險試驗直接接觸，對於實驗人 員之人身安全更加有保障。 爲了達成上述之目的，本發明中係藉由一反應室，以 及至少一試劑室可供容納試劑且與該反應室連通，於該試 劑室與反應室兩者間，係爲閘門室以作爲相互連接，旦於 閘門室中設置有活塞，可控制試劑室與反應室之流通關 係。此外包括一反應物注入口是與反應室相連通，於該反 應物注入口下設置有一被動單向閥，係可允許一測驗樣品 由反應物注入口進入反應室之單向控制，防止混合劑意外 本紙張尺度適用中國國家標準（CNS)A4規格（210 χ 297公莹） ---------------t--------訂---------V (請先閱讀背面之注意事項再填寫本頁) _ Λ7 B7 v 44537 4- 明 說 明 發 五 經濟部%慧財產局—Η消費合作社印製 自該反應室排出。當操作時，將測驗樣品注入反應室後， 依實驗實際需要，釋開封閉試劑室之活塞令儲存於內之試 劑釋入反應室與反應物混合，以進行檢驗之工作。 較佳者，所述之活塞更包括一貫穿之通孔係設置於該 活塞之一對邊中，藉由該通孔可控制該試劑室中之試劑適 當的流入該反應室中。 較佳者，所述之被動單向閥係爲一撓性臂之裝置者， 可以使反應物單向地流入該反應室中。 較佳者，所述之閘門室兩側邊中更包括有一對流道 孔，係分別爲一反應室流道孔及一試劑室流道孔等，可讓 試劑室中之試劑適當的流入該反應室中。 較佳者，更包括有一拋棄式反應模組之製造方法，其 包括有：（a)將一上蓋密封裝置於一主體之上；（b)將一止 逆閥接合於該上蓋中所設置之一止逆閥預留孔之中，可使 反應物能夠藉由該止逆閥單向流入該主體所設置之一反應 室中；（c)將至少一個活塞裝置於該上蓋所設置的至少一活 塞預留孔內，並塞入該主體所設置之至少一閘門室中；（d) 將試劑經由該上蓋中所設置之至少一試劑注入孔而流入於 該主體所設置之複數個試劑室中；（e)再以至少一密封件將 該至少一試劑注入孔加以密封以避免試劑外洩，完成一拋 棄式反應模組。 (四)圖示之簡要說明： 圖一係爲本發明實施例之側視圖。 圖二係爲本發明實施例之側視組合步驟圖。 ! -t 1 i ------I I _t* I I I I I I —tr. (請先閱請背面之注意事項再填寫本頁) 本紙張尺度適闬中國國家標準（CNS)A4規格（MO x 297公釐） 經濟部智慧財產局員工消費合作社印裂 d45374 A7 ___B7—_ 五、發明說明（vf ) 圖三A係爲本發明實施例中有關主體部份之側視圖。 圖三B係爲本發明實施例中有關主體部份之頂視圖。 圖四A係爲本發明實施例中有關上蓋部份之側視圖。 圖四B係爲本發明實施例中有關上蓋部份之頂視圖。 圖五A係爲本發明實施例中有關止逆閥部份之側視 圖。 圖五B係爲本發明實施例中有關止逆閥部份之頂視 圖。 圖六A係爲本發明實施例中有關活塞部份之側視圖。 圖六B係爲本發明實施例中有關活塞部份之前視圖。 圖號說明z ------------- I --------訂. <請先閱讀背面之注意事項再填寫本頁) 10主體 12反應室 14試劑室 14A第一試劑室 14B第二試劑室 14C第三試劑室 16閘門 16A第一閘門 16B第二閘門 16C第三閘門 20上蓋 22止逆閥預留孔 24活塞預留孔 24A第一活塞預留孔 24B第二活塞預留孔 24C第二活塞預留孔 26試劑注入孔 26A第一試劑注入孔 26B第二試劑注入孔. 26C第三試劑注入孔 30止逆閥 32反應物注入口 34撓性臂 40活塞 42定位凸耳 44 0型環 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 2957公发） 445374 A7 __ B7 五、發明說明（y) 46長型環 48通孔 50密封件 161、 163、165反應室流通孔 162、 164、166試劑室流通孔 (五)發明之詳細說明： 本發明係有關於一種拋棄式反應模組，其主要是對於 拋棄式反應模組之結合封裝、控制、及混合微量流體之相 關技術，進而製作爲一種封閉可攜帶式之反應模組。所述 之拋棄式反應模組，係可預先依實驗所需之試劑及實驗步 驟封裝試驗及反應模組，%且可將反應模組之尺寸縮小 化，便於實驗人員攜帶；另^方面，本發明係以射出成型 方法製成反應模組，具有底成本、易組裝等優點。再者， 於完成實驗後，可將反應模組拋棄，是以自實驗開始至實 驗結束，實驗人員無需與危險試驗直接接觸以確保人身安 全。 本發明所述之拋棄式反應模組中，主要係包含一反應 室、至少一試劑室供容納試劑且與該反應室相連通，於試 劑室與反應室兩者之間設置有一閘門室可控制期間之流通 關係，另包括一反應物注入口係與反應室連通，及包括一 被動單向閥允許測驗樣品由反應物注入口進入反應室之單 向控制。當操作時’將反應物注入反應室後，可依實驗需 要’釋開封閉試劑室之活塞令儲存於內之試劑流入反應室 與反應物相混合，以進行檢驗之工作。爲了進一步說明有 關本發明之主要架構，茲配合圖一所示作一說明 本纸張又度適用中國國家標準（CNS)A4規格（210 x 297公釐） (請先閱讀背面之注意事項再填寫本頁) 裝--------訂---------i6 經濟部智慧財產局員工消費合作社印製 ύ^5374 Α7 __Β7 _ 五、發明說明（） 首先，煩請參閱圖一，圖一所示係爲本發明實施例之 側視圖，其中主要係揭示本發明之拋棄式反應模組包括有 一主體10，其中包括有一反應室12用以儲存一反應物，以 及包括有至少一試劑室14用以儲存試劑，另包括有至少一 閘門室16，是可控制該試劑室14與反應室12兩者間之流 通關係。於該主體10之上端係設置有一上蓋20，作爲該主 體1〇之頂蓋用，該上蓋20之中係設置有一止逆閥預留孔 22，至少一活塞預留孔24以及至少一試劑注入孔26(其將 於爾後圖四再作說明)。於該主體10之一端係設置有一止逆 閥30 ’用以允許一測驗樣品由一反應物注入口 32而進入反 應室12之單向控制。此外如圖一所示，更包括有至少一活 塞40，係裝置於該主體10之閘門室16中，可釋開封閉該 複數個試劑室14，使儲存於該試劑室14之試劑能適當的流 入反應室12中而與反應物混合，以進行檢驗之工作。並且 於所述之試劑注入孔26之上係設置有密封件50，用以將該 試劑注入孔26加以密封以防止試劑洩漏於外。 經濟部智慧財產局員工消費合作社印Α'ιβ _ I! —I tp I n n .—r ^ I · i I n n n 一SJi {請先閱讀背面之注意事項再填寫本頁) 本發明亦包括有一抛棄式反應模組之製造方法，及如 圖二所示之實施步驟，該圖二之所示係爲本發明實施例之 側視組合步驟圖，其歩驟主要係包括有；（a)將一上蓋20 密封裝置於一主體10之上；（b)將一止逆閥30接合於該上 蓋20中所設置之一止逆閥預留孔22之中，可使反應物能 夠藉由該止逆閥20單向流入該主體所設置之一反應室12 中；（c)將至少一個活塞40裝置於該上蓋20所設置的至少 一活塞預留孔24內，並塞入該主體10所設置之至少一閘 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 297公餐） 74 74 經濟部智慧財產局員工消費合作社印製 A7 __B7____ 五、發明說明（7〉 門室16中；（d)將試劑經由該上蓋20中所設置之至少一試 劑注入孔26而流入於該主體10所設置之複數個試劑室14 中；（e)再以至少一密封件50將該至少一試劑注入孔26加 以密封以避免試劑外洩。藉由上述之步驟’以完成一拋棄 式反應模組。 爲了更清楚說明本發明所述之結構，茲配合各部份之 結構圖式詳加說明，煩請參閱圖三，圖三所示係爲本發明 實施例中有關主體10部份之結構示意圖，其中圖三A所示 係爲本發明實施例中有關主體10部份之側視圖，而圖三B 所示係爲本發明實施例中有關主體10部份之頂視圖。如圖 三A所示者’該主體10中係包括有一反應室12，用以置放 所欲測試之反應物。另外設置有複數個試劑室14，用以置 放執行檢驗工作所需之試劑，如第一試劑室14A、第二試劑 室14B以及第三試劑室14C等等，可以存放多種不同之試 劑。於本發明實施例中係以三個試劑室爲例子作說明，實 驗人員當可配合其本身實際檢驗之需求，而增加試劑室之 數目。 另一方面，如圖三B之所示，於該反應室12與複數各 試劑室14之間係設置有複數個鬧門室16，例如有第一鬧門 室16A、第二閘門室16B、第三閘門室16C等等，其設置係 與該複數個試劑室i4A、14B、14C相對應，其主要係用以 置放複數個活塞40，可藉以控制該反應室12與複數個試劑 室14之間的流通與流量之關係。此外，介於該主體之 各個聞門室16之的兩側邊中更包括有一對流道孔ία、 ------------- ^--------訂· (請先閱讀背面之注意事項再填寫本頁) 本紙張尺度適用中國國家標準（CNS)A4規格（210 X 29?公餐) 4453?4 A7 ____B7_ 五、發明說明（β) 162 ’係分別爲一反應室流道孔161及一試劑室流道孔162 等’可讓複數個試劑室14A、14B、14C中之試劑適當的流 入該反應室12中。所以配合該第二閘門室16B之兩側邊係 同樣設置有一反應室流道孔163及一試劑室流道孔164;同 樣地，該第三閘門室16C之兩側邊係同樣設置有一反應室 流道孔165及一試劑室流道孔166。此外，對於所述之複數 個流道孔161、162而言，其主要係可配合該活塞40中所 設置之通孔48(顯示於圖六中)，可進一步控制該複數個試劑 室14A、14B、14C中之藥劑適當的流入該反應室12中。 如此而完成本發明所述之主體10架構，該主體10是可爲 一整體射出成形之結構者，藉以有效降低成本。 經濟部智慧財產局員工消費合怍社印製 ---------------裝--------訂， (請先閱讀背面之注意事項再填寫本頁) 煩請參閱圖四，圖四所示係爲本發明實施例中有關上 蓋部份之結構示意圖，其中該圖四A所示係爲本發明實施 例中有關上蓋部份之側視圖，而圖四B所示係爲本發明實 施例中有關上蓋20部份之頂視圖。誠如該圖四A之所示， 該上蓋20中係包括有一止逆閥預留孔22，用以置放一止逆 閥30者；以及包括複數個活塞預留孔24，其爲第一活塞預 留孔24A、第二活塞預留孔24B以及第三活塞預留孔24C等 等，係用以置放複數個活塞40，於本發明實施例中係以三 個活塞爲實施之方式者。於該上蓋2G中亦包括有複數個試 劑注入孔26，意即爲第一試劑注入孔26A、第二試劑注入 孔26B以及第三試劑注入孔26C等等，藉以配合該複數個 試劑室14A、14B、14C等之將試劑儲放入試劑室中。如此 而完成本發明所述之上蓋20架構，其中該上蓋20係可爲 1本紙張&度適ifl帽國家&準（CNS)A4規格⑵〇 X β公爱) 445374 Λ7 五、發明說明（/) 一整體射出成形者，藉以節省製作成本。 煩請參閱圖五，圖五所示係爲有關本發明實例中所述 之止逆閥30的結構示意圖，其中圖五A所示係爲本發明實 施例中有關止逆閥3G部份之側視圖，而圖五B則係有關於 本發明實施例中止逆閥30部份之頂視圖。如圖所示，該止 逆閥30中係包括有一反應物注入口 32，用以放入所欲測試 之反應物，於該反應物注入口 32之下端係設置連接有一撓 性臂34，其爲一被動單向閥之裝置形式，係可控制該反應 物僅能夠單向地注入該反應室12中，防止混合劑意外自反 應室12排出。如此而完成本發明所述之止逆閥30結構。 經濟郤智慧財產局員工消費合作社印製 I ---I -----— II - I t I I I —^_ (請先閱讀背面之注意事項再填寫本頁) 煩請參閱圖六，圖六所示係爲本發明所提出之活塞40 結構示意圖，其中圖六A係爲本發明實施例中有關活塞40 部份之側視圖，而圖六B係爲本發明實施例中有關活塞40 部份之前視圖。於本發明實施例中配合圖一、二之所示可 知，其使用有三個活塞裝置藉以配合三個試劑室之流通控 丨 制。今將以單一活塞4G爲例子作一結構方式之說明，如圖 六A之所示，該活塞40之側視外觀圖樣係爲一 T型之形狀。 此外，於該活塞40中係包括有一對定位凸耳42，其設置係 爲左右對稱於該活塞4G之設置方式，藉以使該活塞40能 夠定位於該上蓋20之活塞預留孔24中者。該活塞40中更 包括有一 0型環’係與該活塞40 —體成形者，可使得該活 塞40能夠緊密地裝置於該主體1〇所設置之閘門室16中。 此外，該活塞40中更包括有一長型環46，亦同樣地是與該 活塞40 —體成形者，同樣地，係能夠使該活塞4G緊密地 本紙張尺度適用中國國家標準（CNSM4規格（_2i〇 χ 29户公i j ------- 經濟部智慧財產局員工消費合作社印製 445374 A7 ___B7 五、發明說明（P) 裝置於該主體10所設置之閘門室16中。 然而，該長型環46之中係更包括有一貫穿之通孔48， 則該活塞40即可藉由該通孔48而能夠控制該複數個試劑 室14A、14B、14C中之試劑適當地流入該反應室12中。其 中該通孔48係對照於該閘門室16中所設置之反應室流通 孔161、以及試劑室流通孔162等，即可有效控制該試劑適 當地流入該反應室12中。如此而完成本發明所述之活塞40 結構。 經由上述之說明，可清楚了解到本發明所提出之一拋 棄式反應模組，其可供使用者依實驗需要，於一反應模組 內提供兩種或兩種以上之試劑執行測試之工作。亦可依實 驗步驟之需要，控制該閘門室16中之活塞40 —次釋放一 種或多種試劑。另一方面，前述主體10結構之材質係可以 透光材質而製成拋棄式反應模組者；並且於反應完成後， 直接將拋棄式反應模組置於光度計或光譜儀中進行光學檢 另對於本發明之運用領域而言，使用者當可將拋棄式 反應模組擴大應用至水質、血液及尿液等生化檢驗中。並 且可依實驗之實際需要，可將混合劑自反應室中取出，以 進行其他檢驗。是以藉由本發明之拋棄式反應模組，係可 預先依實驗所需之試劑及實驗步驟封裝試驗及反應模組。 再者’可一實驗人員之實際需要，將該拋棄式反應模組之 尺寸縮小化，便於實驗人員攜帶。而所述之各部結構是以 射出成型方法製成一反應模組，具有底成本、易組裝等優 -------------.t--------訂 (請先閱讀背面之注音？事項再填寫本頁) 本紙張尺度適用中國國家標準（c'ns)A4規格（21〇χ L#公t ) 445374 A7 ___B7____ 五、發明說明（（丨） 點。當完成實驗後，可將反應模組拋棄，所以自實驗開始 至實驗結束，實驗員無需與危險試驗直接接觸，以保障實 驗人員之人身安全。 綜上所述，本發明之一種拋棄式反應模組與方法，其 主要目的是有效改善以往實驗人員須準備燒杯、試管及滴 管之攜帶不便旦容易破碎之缺點，以及減少若不熟悉操作 程序則有危險產生等等的缺失，充份顯示出本發明之目的 及功效與技術特徵上均深富實施之進步性，極具產業之利 用價值，旦爲目前市面上前所未見之新發明，完全符合發 明專利之要件，爰依法提出申請。 唯以上所述者，僅爲本發明之較佳實施例而已，當不 能以之限定本發明所實施之範圍。即大凡依本發明申請專 利範圍所作之均等變化與修飾，皆應仍屬於本發明專利涵 蓋之範圍內’謹請貴審查委員明鑑，並祈惠准，是所至 禱。 —----ΙΓ — I---- 111 ----訂. (請先閱讀背面之注意事項再填寫本頁) 經濟部智慧財產局員工消費合作社印製 本紙張ϋ適用中國國家標準（CNS)A4規格公楚）445374 Π: Description of the invention (A7 B7 (1) Technical field of the invention: (Please read the precautions on the back before filling out this page) The present invention relates to disposable reaction modules, especially a reaction module for trace fluids. A reaction tool and a plurality of reagent chambers are set up and the flow relationship between them can be controlled to achieve a high safety, convenience, and mobility testing tool. (II) Background of the Invention: The current application of general chemical / biochemical As for inspection tools, when a user wants to perform field water quality test, the experimenter must bring the required reagents, measuring cups, test tubes, droppers and other equipment to the field. However, these experimental equipment are usually stored in In fragile containers or made of fragile materials, it is inconvenient to handle. And when handling dangerous reagents, it may also be contaminated with reagents due to negligence or unfamiliar with the operating procedures, which has potential danger for laboratory personnel. The impact on personal safety is huge, and after completing the processing test of the reactant and reagent mixture to be tested, it may also cause pollution to the environment. The present invention will improve the above-mentioned shortcomings, and then propose a disposable reaction module to facilitate the use of experimental personnel. (3) Brief description of the invention: The Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs printed this invention. The main purpose is to provide a disposable reaction module, especially a trace fluid reaction module, in which the reagent and the reactant are mixed only when the reagent is enclosed in an entire module, To provide a chemical / biochemical inspection tool with high safety, convenience, and mobility. Another object of the present invention is to provide a disposable reaction module, which can be conveniently carried by experimenters to the field for a predetermined test. Ϊ́ The paper size applies the Chinese National Standard (CNS) A4 specification (210 X ^ 7 mm) Printed by the Intellectual Property Bureau of the Ministry of Economic Affairs, BT Industrial Consumer Cooperative, 5314 Α7 ____ Β7 _____ 5. Description of the invention (>) Another object of the present invention is to When the disposable reaction module of the present invention is used, the step of measuring reagents can be removed, and the time for inspection is greatly reduced. A further object of the present invention is to provide a method for manufacturing the disposable reaction module, which can seal the reagents required for a predetermined experiment into the reaction module according to different needs of the experimenter. Another object of the present invention is to propose a disposable The reaction module can be used by the user to package the test and reaction module in advance according to the reagents and experimental steps required for the experiment. Another object of the present invention is to provide a disposable reaction module, which is a disposable reaction module. The size is reduced, which is convenient for the experimenter to carry. Another object of the present invention is to propose a disposable reaction module, which is made by a method of integral injection molding, which has the advantages of low cost and easy assembly. To improve the lack of conventional technology carrying and constant operation. The second purpose of the present invention is to propose a disposable reaction module, which can be discarded after the experiment is completed. Therefore, from the beginning of the experiment to the end of the experiment, the experimental entrants do not need to be in direct contact with the dangerous test, and the personal safety of the experimental personnel is more guaranteed. In order to achieve the above-mentioned object, in the present invention, a reaction chamber and at least one reagent chamber are capable of containing reagents and communicating with the reaction chamber. Between the reagent chamber and the reaction chamber, a gate chamber is used as a mutual Connected, once a piston is set in the gate chamber, the flow relationship between the reagent chamber and the reaction chamber can be controlled. In addition, a reactant injection port is communicated with the reaction chamber. A passive one-way valve is provided under the reactant injection port, which is a one-way control that allows a test sample to enter the reaction chamber from the reactant injection port to prevent mixing. Unexpectedly, the size of this paper applies the Chinese National Standard (CNS) A4 specification (210 x 297 Gongying) --------------- t -------- Order ----- ---- V (Please read the notes on the back before filling out this page) _ Λ7 B7 v 44537 4-State that the Fifth Ministry of Economic Affairs% Hui Property Bureau-ΗConsumer Cooperative will print and discharge from this reaction chamber. When in operation, after injecting the test sample into the reaction chamber, according to the actual needs of the experiment, release the piston that closed the reagent chamber and let the reagent stored in it be released into the reaction chamber and mixed with the reactants for inspection. Preferably, the piston further includes a penetrating through-hole disposed in one of the opposite sides of the piston, and the reagent in the reagent chamber can be appropriately flowed into the reaction chamber through the through-hole. Preferably, the passive one-way valve is a device with a flexible arm, which can make the reactants flow into the reaction chamber unidirectionally. Preferably, a pair of flow passage holes are included on both sides of the gate chamber, which are a reaction chamber flow passage hole and a reagent chamber flow passage hole, respectively, so that the reagent in the reagent chamber can properly flow into the reaction. Room. Preferably, it also includes a manufacturing method of a disposable reaction module, which includes: (a) sealing an upper cover on a main body; (b) joining a check valve to the upper cover. A check valve reserved hole allows reactants to flow unidirectionally into a reaction chamber provided in the main body through the check valve; (c) at least one piston is installed in at least one of the upper cover. The piston is reserved in the hole and plugged into at least one gate chamber provided in the main body; (d) the reagent is flowed into the plurality of reagent rooms provided in the main body through the at least one reagent injection hole provided in the upper cover; (E) The at least one reagent injection hole is sealed with at least one seal to prevent leakage of the reagent, and a disposable reaction module is completed. (IV) Brief description of the figure: Figure 1 is a side view of an embodiment of the present invention. FIG. 2 is a side-view combination step diagram of an embodiment of the present invention. ! -t 1 i ------ II _t * IIIIII —tr. (Please read the notes on the back before filling in this page) This paper is suitable for Chinese National Standard (CNS) A4 (MO x 297 mm) (%) The consumer cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs d45374 A7 ___B7 — _ 5. Description of the invention (vf) Figure 3A is a side view of the main part of the embodiment of the present invention. FIG. 3B is a top view of the main part of the embodiment of the present invention. FIG. 4A is a side view of the upper cover part in the embodiment of the present invention. FIG. 4B is a top view of the upper cover portion in the embodiment of the present invention. FIG. 5A is a side view of a non-return valve portion in the embodiment of the present invention. Fig. 5B is a top view of a non-return valve portion in the embodiment of the present invention. FIG. 6A is a side view of the piston portion in the embodiment of the present invention. FIG. 6B is a front view of the piston portion in the embodiment of the present invention. Description of drawing number z ------------- I -------- order. ≪ Please read the notes on the back before filling this page) 10 main body 12 reaction room 14 reagent room 14A first reagent chamber 14B second reagent chamber 14C third reagent chamber 16 gate 16A first gate 16B second gate 16C third gate 20 upper cover 22 check valve reserved hole 24 piston reserved hole 24A first piston reserved hole 24B second piston reserve hole 24C second piston reserve hole 26 reagent injection hole 26A first reagent injection hole 26B second reagent injection hole 26C third reagent injection hole 30 check valve 32 reactant injection port 34 flexible arm 40 piston 42 positioning lug 44 0 ring This paper size applies to Chinese National Standard (CNS) A4 specification (210 X 2957) 445374 A7 __ B7 V. Description of the invention (y) 46 long ring 48 through hole 50 seal 161, 163, 165 reaction chamber flow holes 162, 164, 166 reagent chamber flow holes (5) Detailed description of the invention: The present invention relates to a disposable reaction module, which is mainly a combination packaging of the disposable reaction module , Control, and mixing of microfluidic related technologies, and then made into a closed and portable Reaction module. The disposable reaction module can be used to package the test and reaction module in accordance with the reagents and experimental steps required for the experiment in advance, and the size of the reaction module can be reduced, which is convenient for the experimenter to carry; The invention is a reaction module made by injection molding, which has the advantages of low cost and easy assembly. In addition, after completing the experiment, the reaction module can be discarded. From the beginning of the experiment to the end of the experiment, the experimenter does not need to directly contact the dangerous test to ensure personal safety. The disposable reaction module according to the present invention mainly includes a reaction chamber and at least one reagent chamber for containing reagents and is in communication with the reaction chamber. A gate chamber is provided between the reagent chamber and the reaction chamber to control the gate chamber. The flow relationship during this period also includes a one-way control of a reactant injection port communicating with the reaction chamber and a passive check valve that allows a test sample to enter the reaction chamber from the reactant injection port. When the reagent is injected into the reaction chamber during the operation, the piston closed the reagent chamber can be released according to the needs of the experiment, and the reagent stored therein can be flowed into the reaction chamber and mixed with the reactants for inspection. In order to further explain the main structure of the present invention, we will make a description with reference to Figure 1. This paper is also applicable to the Chinese National Standard (CNS) A4 specification (210 x 297 mm) (Please read the precautions on the back before filling out (This page) Install -------- Order --------- i6 Printed by the Intellectual Property Bureau Employee Consumer Cooperatives of the Ministry of Economic Affairs ^ 5374 Α7 __Β7 _ V. Description of the invention () First, please refer to the figure First, FIG. 1 is a side view of an embodiment of the present invention, which mainly discloses that the disposable reaction module of the present invention includes a main body 10 including a reaction chamber 12 for storing a reactant, and including at least A reagent chamber 14 is used for storing reagents, and further includes at least one gate chamber 16, which can control the flow relationship between the reagent chamber 14 and the reaction chamber 12. An upper cover 20 is provided on the upper end of the main body 10 as a top cover of the main body 10, and a check valve reserved hole 22, at least one piston reserved hole 24 and at least one reagent injection are provided in the upper cover 20. Hole 26 (which will be described later in Figure 4). A non-return valve 30 'is provided at one end of the main body 10 for one-way control of allowing a test sample to enter the reaction chamber 12 through a reactant injection port 32. In addition, as shown in FIG. 1, it further includes at least one piston 40, which is installed in the gate chamber 16 of the main body 10, and can release and close the plurality of reagent chambers 14 so that the reagents stored in the reagent chamber 14 can be appropriately It flows into the reaction chamber 12 and is mixed with the reactants for inspection. A sealing member 50 is provided on the reagent injection hole 26 to seal the reagent injection hole 26 to prevent the reagent from leaking to the outside. Printed by the Consumers ’Cooperative of Intellectual Property Bureau of the Ministry of Economic Affairs Α'ιβ _ I! —I tp I nn. —R ^ I · i I nnn SJi {Please read the notes on the back before filling this page) The invention also includes a discard The manufacturing method of the reaction module and the implementation steps shown in Figure 2. The figure in Figure 2 is a side-view combination step diagram of the embodiment of the present invention, and its steps mainly include: (a) a The upper cover 20 is sealed on a main body 10; (b) a check valve 30 is engaged in a check valve reserved hole 22 provided in the upper cover 20, so that reactants can pass through the check The valve 20 flows unidirectionally into a reaction chamber 12 provided in the main body; (c) at least one piston 40 is installed in at least one piston reserved hole 24 provided in the upper cover 20 and plugged into the main body 10 At least one paper size of this paper applies the Chinese National Standard (CNS) A4 specification (210 X 297 meals) 74 74 Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs A7 __B7____ V. Description of the invention (7> In the door 16; (d The reagent flows through at least one reagent injection hole 26 provided in the upper cover 20 Into the plurality of reagent chambers 14 provided in the main body 10; (e) the at least one reagent injection hole 26 is sealed with at least one seal 50 to prevent the reagent from leaking out; by the above steps, a complete Disposable reaction module. In order to explain the structure of the present invention more clearly, the structure diagram of each part will be explained in detail. Please refer to FIG. 3. FIG. 3 shows the main body 10 in the embodiment of the present invention. Fig. 3A is a side view of the main body 10 part in the embodiment of the present invention, and Fig. 3B is a top view of the main body 10 part in the embodiment of the present invention. As shown in FIG. 3A, the main body 10 includes a reaction chamber 12 for placing a reactant to be tested. In addition, a plurality of reagent chambers 14 are provided for placing reagents required for performing inspection work. For example, the first reagent chamber 14A, the second reagent chamber 14B, the third reagent chamber 14C, etc. can store a variety of different reagents. In the embodiment of the present invention, three reagent chambers are taken as an example for illustration. Actual inspection On the other hand, as shown in FIG. 3B, a plurality of alarm doors 16 are provided between the reaction chamber 12 and a plurality of reagent chambers 14, such as a first alarm door. The chamber 16A, the second gate chamber 16B, the third gate chamber 16C, etc. are arranged corresponding to the plurality of reagent chambers i4A, 14B, 14C, and are mainly used to place a plurality of pistons 40, thereby controlling the The relationship between the flow and the flow rate between the reaction chamber 12 and the plurality of reagent chambers 14. In addition, a pair of flow passage holes ία, ------ are included on both sides of each of the door chambers 16 of the main body. ------- ^ -------- Order · (Please read the precautions on the back before filling this page) This paper size applies to China National Standard (CNS) A4 (210 X 29? Meals) ) 4453? 4 A7 ____B7_ V. Description of the Invention (β) 162 'Respectively is a reaction chamber flow channel hole 161 and a reagent chamber flow channel hole 162, etc.' can allow reagents in a plurality of reagent chambers 14A, 14B, 14C to be appropriate Into the reaction chamber 12. Therefore, a reaction chamber flow passage hole 163 and a reagent chamber flow passage hole 164 are also provided on both sides of the second gate chamber 16B; similarly, a reaction chamber is provided on both sides of the third gate chamber 16C. A flow passage hole 165 and a reagent chamber flow passage hole 166. In addition, for the plurality of flow passage holes 161, 162, it is mainly possible to cooperate with the through holes 48 (shown in FIG. 6) provided in the piston 40, and further control the plurality of reagent chambers 14A, The medicaments in 14B and 14C flow into the reaction chamber 12 appropriately. In this way, the structure of the main body 10 according to the present invention is completed, and the main body 10 is a structure that can be integrally molded, thereby effectively reducing costs. Printed by the Intellectual Property Bureau of the Ministry of Economic Affairs of the Consumers ’Union ------------ Order, (Please read the precautions on the back before filling this page Please refer to Figure 4. Figure 4 is a schematic diagram of the structure of the upper cover portion in the embodiment of the present invention, wherein Figure 4A is a side view of the upper cover portion in the embodiment of the present invention, and Figure 4 B is a top view of the upper cover 20 in the embodiment of the present invention. As shown in FIG. 4A, the upper cover 20 includes a check valve reserve hole 22 for placing a check valve 30; and a plurality of piston reserve holes 24, which are first The piston reserved hole 24A, the second piston reserved hole 24B, the third piston reserved hole 24C, etc. are used to place a plurality of pistons 40. In the embodiment of the present invention, three pistons are used as the implementation method. . The upper cover 2G also includes a plurality of reagent injection holes 26, that is, a first reagent injection hole 26A, a second reagent injection hole 26B, a third reagent injection hole 26C, etc., so as to cooperate with the plurality of reagent chambers 14A, 14B, 14C, etc. Store the reagents in the reagent chamber. In this way, the structure of the upper cover 20 according to the present invention is completed, wherein the upper cover 20 can be a piece of paper & degree ifl hat country & quasi (CNS) A4 size (⑵OX β public love) 445374 Λ7 5. Description of the invention (/) Shooting the shaper as a whole to save production costs. Please refer to FIG. 5. FIG. 5 is a schematic structural diagram of the check valve 30 described in the example of the present invention, and FIG. 5A is a side view of the check valve 3G part in the embodiment of the present invention. FIG. 5B is a top view of a portion of the check valve 30 in the embodiment of the present invention. As shown in the figure, the check valve 30 includes a reactant injection port 32 for receiving the reactant to be tested. A flexible arm 34 is connected to the lower end of the reactant injection port 32. It is a device form of a passive one-way valve, which can control the reactant to be injected into the reaction chamber 12 only one way, and prevent the mixture from being accidentally discharged from the reaction chamber 12. In this way, the structure of the check valve 30 according to the present invention is completed. Printed by the Economic and Intellectual Property Bureau Staff Consumer Cooperatives I --- I -----— II-I t III — ^ _ (Please read the notes on the back before filling this page) Please refer to Figure 6 and Figure 6 Shown is a schematic diagram of the structure of the piston 40 proposed in the present invention, wherein FIG. 6A is a side view of the piston 40 portion in the embodiment of the present invention, and FIG. 6B is the piston 40 portion before the embodiment of the present invention. view. In the embodiment of the present invention, as shown in Figs. 1 and 2, it can be seen that it uses three piston devices to cooperate with the flow control of the three reagent chambers. Now, a single piston 4G will be used as an example to explain the structure. As shown in FIG. 6A, the side appearance of the piston 40 is a T-shape. In addition, a pair of positioning lugs 42 are included in the piston 40, and the arrangement is symmetrical to the piston 4G, so that the piston 40 can be positioned in the piston reserved hole 24 of the upper cover 20. The piston 40 further includes an O-ring, which is integrally formed with the piston 40, so that the piston 40 can be tightly installed in the gate chamber 16 provided in the main body 10. In addition, the piston 40 further includes a long ring 46, which is the same as the body forming the piston 40. Similarly, the piston 4G can be made tightly. The paper size applies the Chinese national standard (CNSM4 specification (_2i 〇χ 29 households ij ------- Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs 445374 A7 ___B7 V. Description of the invention (P) The device is installed in the gate chamber 16 provided in the main body 10. However, the chief The ring 46 further includes a through hole 48 therethrough, so that the piston 40 can control the reagents in the plurality of reagent chambers 14A, 14B, and 14C to appropriately flow into the reaction chamber 12 through the through hole 48. The through hole 48 is compared with the reaction chamber flow hole 161 and the reagent chamber flow hole 162 provided in the gate chamber 16 to effectively control the reagent to properly flow into the reaction chamber 12. This is completed The structure of the piston 40 according to the present invention. Through the above description, one can clearly understand one of the disposable reaction modules proposed by the present invention, which can be used by users to provide two or two in one reaction module according to experimental needs. More than one reagent The work of testing. It is also possible to control the piston 40 in the gate chamber 16 to release one or more reagents at a time according to the needs of the experimental steps. On the other hand, the material of the structure of the main body 10 can be made of a light-transmitting material and made a disposable reaction. After the reaction is completed, the disposable reaction module is directly placed in a photometer or spectrometer for optical inspection. In addition, for the application field of the present invention, the user can expand the disposable reaction module to Water, blood, urine and other biochemical tests. And according to the actual needs of the experiment, the mixture can be taken out of the reaction chamber for other tests. By using the disposable reaction module of the present invention, The reagents and experimental steps required for the experiment encapsulate the test and reaction module. Furthermore, the size of the disposable reaction module can be reduced according to the actual needs of the experimenter, which is convenient for the experimenter to carry. The structure of each part is An injection molding method is used to make a reaction module, which has the advantages of low cost and easy assembly .-------------. T -------- Order (Please read the note on the back first Matters again (Fill in this page) This paper size is in accordance with Chinese national standard (c'ns) A4 specification (21〇χ L # 公 t) 445374 A7 ___B7____ 5. Description of invention ((丨) point. After completing the experiment, you can set the reaction module Discard, so from the beginning of the experiment to the end of the experiment, the experimenter does not need to directly contact the dangerous test to ensure the personal safety of the experimenter. In summary, the main purpose of the disposable reaction module and method of the present invention is to effectively improve In the past, experimenters had to prepare beakers, test tubes and droppers, which were inconvenient to carry and easy to break, and to reduce the lack of danger if they were unfamiliar with the operating procedures, etc., fully showing the purpose, efficacy and technical characteristics of the present invention. Both of them are fully implemented and have great industrial use value. As a new invention that has not been seen on the market today, it fully meets the requirements of an invention patent and applies according to law. The above are only the preferred embodiments of the present invention, and it should not be used to limit the scope of implementation of the present invention. That is to say, all equal changes and modifications made in accordance with the scope of the patent application for the present invention should still fall within the scope of the patent of the present invention. —---- ΙΓ — I ---- 111 ---- Order. (Please read the notes on the back before filling out this page) This paper is printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs, which applies Chinese national standards ( CNS) A4 specifications