TW442496B - Processing and applications of fungal mycelia - Google Patents

Abstract

Fiber residues obtained by comminution, acidic or alkaline treatment, and depigmentation from fungal mycelia or its extracted residuals, such a ""ling-zhi"", have been found many applications in medicinal, pharmaceutical and cosmetic field. The chemical analysis reveals that fibers is composed of β-(1,3)-D-glucan and N-acetylglucosamine. Fibers so obtained can be freeze-dried into thin and porous sheets for use as the skin substitute with the ab y to accelerate the healing of skin trauma; fibers can also be the additives to the cosmetic products for the purpose of skin cares; fibers can further be processed and sieved during production or after drying to employ as the pharmaceutical excipients.

Description

442496 Α7 Β7 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs. 5. Description of the Invention (1) 1 Field of Industrial Use The present invention is the research and development of true plants. Artificial skin, skin repair and maintenance, and use as a medicinal ingredient :.知 知 故 #f. Due to the importance of raw sauce materials in the application of medicine, the development of biomedical materials has been studied for a long time. Among these sound-generating materials, albumin and Mingsheng have been used in the carrier formulations of drugs to improve the therapeutic energy of drugs. In recent years, collagen, deacetyi chitin (crhitoSiiii), and chitin (Chitin) have become the most important ingredients in cosmetics. More importantly, the disadvantages and disadvantages of traditional wound closure treatment have improved with the development of raw materials. As early as 15 1943, the gold from the knees could be extracted from animal gold, and obtained the purified state of different skins, or converted to thin, or applied to wound healing by other structural methods. In 1970, Prudden et al. Published a method for accurate technical analysis. The poor poly (N-Acety 1Glucos-amine) polymer containing N-Acety 1Glucos-amine can be used locally in the laboratory. The skin has a growth capacity of 20, and it is clearly found to be superior to cartilage. Therefore * a new era of research on chitin's ability to heal wounds is launched. BESCHITIN W products produced by MORITIHITA RESERE are manufactured by Chiyin Leather and processed by chitin. A product of 产物, the company said that BESCHITIN W exhibits air penetrability and moisture. 3 paper sizes are applicable to China National Standard (CNS) A4 (210X297 mm) (please read the back first) Please pay attention to this page, please fill out this page) Order! -442496 A7 B7 V. Description of the invention (2) 1 The adsorption force is all collagen, and said that chitin warming has a very high affinity for the surface of the skin, which is more important Its price is still normal to maintain the skin tissue proliferation ability. 5 However, the use of chitin for making ravioli is warmer than that of BESCHITIN W, and usually requires a complicated and easy to dye environment. Firstly, it needs to be processed by acid or strong test to receive other non-essential costs and then purify to obtain high-purity chitin. It is still necessary to dissolve the purified chitin in an appropriate solvent, and then draw and spin. In order to obtain the proper characteristics of the abdominal garments, the material must be sterilized for clinical use. The strong acids used in the manufacturing process include gallic acid, sparse acid, or scaly acid, etc., but there are not many solvents that can dissolve chitin, which are more often restricted by patents. The final spinning and weaving process also makes renting more complicated. . Therefore, it is necessary to find a process that is more environmentally friendly, so as to develop more pure chitin purity and finished products. Or develop a material with a similar structure, and ease the process to make dressings with artificial skin or better. Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling out this page). We also know that the cell wall of the stupid plant zizhao contains 1-40% chitin, which is mostly multi-lineage. There is a multi-record structure of (1-3) polyglucose (/ 3- (1-3) 20 -D-Glucan). Multi-boiled ingredients can be obtained from many plants, such as known in the Ganoderma plant Contains a variety of substances that can stab the non-specific guilt-free system such as activating giant mouth cells. According to the data of Czop et al., It is easy to accept the acceptance of a macrophage surface (1-3) poly24 inulin / 3-(1-3) -D_G lucan) series of single parent-specific sexual stimulation. Xu " 4 This paper size applies to China National Standard (CNS) A4 (210X297 mm) 442496 A7 B7 Central Bureau of Standards, Ministry of Economic Affairs Printed by Employee Consumer Cooperatives V. Description of Invention (3) 1 Most of the strategies from fungi contain multiple training ceremonies with> 3- (1-3) health. They have been used in Asia, especially in difficult times and Japanese. It is widely used for the application of various symptoms including cancer treatment, etc. In addition, the benefits obtained from oats and barley Food is mainly used for skin care of ί smoothening softener. Furthermore, (1 -5 3) polyglucose (/ 5- (1-3) -D-Glucan) has been shown to have ultimate multifunctional properties such as wounds. Healing and anti-smashing reduction. The lesson to be solved Zhao 10 Ganoderma tsuage has long been important in Asia-a kind of medicinal stupid, especially China and Japan. However, it is obtained by using hot water to extract ganoderma. Water-insoluble fractions (more than 90 0 are usually not used or discarded. Although the fine structure of the Ganoderma lucidum mycelium fraction is still fully analyzed, (1-3) poly inulin (> S-(1 -3) The possible effect of -D-Glucan) and the synergistic effect of chitin15 have made it an ideal raw material for wound anesthesia. In addition, the residual part extracted from Ganoderma lucidum contains many fungi The cell weaving of the fine running wall can be directly woven into a film without dissolving and separating the fine weaving and maintaining process: the violation of the process and fewer purification problems; 5L net dyeing environmental problems. It is a traditional medicine, which is quite safe and does not have any harmful components. It is used in The potential of various aspects and its inherent advantages fully encourage us to develop this product. Objectives of the invention (please read the notes on the back before filling this page) 24 5 This paper standard is applicable to China National Standards (CNS) ) 8 4 specifications (210 × 297 mm) 442496 A7 B7 Printed by Shellfish Consumer Cooperation of the Central Standards Bureau of the Ministry of Economic Affairs. 5. Description of the invention (4) 1 The present invention discloses a special process for purifying stupid mycelium to obtain polyglucose. Amine fiber, which has a weaving dimension of (1-3) polyglucose (/ 3- (1-3) -D-Glucan) and N-Acetylglucosamine copolymer structure 5 The invention also discloses the preparation of polyglucosamine as a kind of warm clothing-SACCHACHITIN, the uses of which include skin substitutes and physical properties. 10 Means taken to solve the problem The process for purifying the mycelium of jasmine or its extracted dregs developed by the present invention is to take fresh stupid or its residues, crush them and diffuse them in an organic solvent, collect the residuals and then The main line is digested with sodium hydroxide, and then decolorized and washed with hypochlorite 15 (screening for the appropriate length in the residual method). The fiber is thinned after being checked. The organic solvents used include low carbon, such as methyl alcohol, ethyl alcohol, and acrylic acid. The polyglucosamine fiber of the present invention, or prepared into various abdominal garments through various conventional techniques, can be applied to artificial skins and skin substitutes to assist wound healing, and can be used as a pharmaceutical agent for anti-drying or pulverization. It can be added to various pharmaceutical agents to improve the physical properties of powders. It can also be used as an excipient; it can also be added to cosmetic preparations as a cosmetic excipient. Pharmaceutical excipients to improve the signing of Fandong 6 (please read the precautions on the back before filling this page) Order-This paper size applies the Chinese National Standard (CNS) A4 specification (210 > < 297mm >); 442496

A7 B7 'Invention description (5 Residuals: to curative effect. ---- 5 ο 1 5 1 Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs 20) Example of the table: Table i Polyglucosamine and Ethylamine ( Acetaminophen) Physical properties_Langhe prescription combination v Table 2 Polyglucose according to the quality of the tablets pressed after t warm water treatment Table 3 Comparison of the physical properties of polyacetamine tablets after 85 "water treatment Comparison Table 4 Physical properties comparison of polyglucosamine fiber after 1N sodium hydroxide treatment Table 5 Polyglucosamine weave and crystalline cellulose co-dried powder formula combination Table 6 Polyglucosamine fiber and B (Acetaminophen) and Weiyi Qixisu in different proportions of paper crystal cellulose together dry powder formula combination Table 7 Polyglucose according to weaving Wei and crystalline cellulose dry powder formula combination powder comparison table 8 Poly Comparison of physical properties of common mainline powders blended with different proportions of Acetaminophen and Acetaminophen and microcrystalline chitin in Table 9 > A (2 丨 0 × 297 mm) 442496 A7 B7 V. Description of the invention 6) 1 · Comparison of the physical properties of Hezhi's missing tablets. Table 10 Comparison of physical properties of common dry media powders in which glucosamine weaving and Acetaminophen and Huanjing weaving vitamins are now combined. 5 Schematic description: 10 15 Printed by the Consumer Cooperatives of the Central Bureau of Procurement, Ministry of Economic Affairs 20 24. Thin layer analysis of SACCHACHITIN, after hydrolysis with 6N hydrochloric acid, ethyl acetate: isopropyl alcohol ίWater = 1: 1: 3 Naphtha m-benzene second-grade " (Naphtharesorcinol) warfare agent (B) or Elson-Morgen reagent as grain agent , And then heated to 100 C for 3 minutes. 1. Sample Table 1 (Sample-1) 2. Sample 2 (Sample_2) 3. Glucose (G1 ucose) 4. Mannose 5. Galactose (6) Ν " * N-Acetylgiucosamine 7. Glucosamine · (G1 ucosam i ne) 8 This paper size is applicable to China National Standard (CNS) A4 specification (210 X 297) (please read the precautions on the back first) (Fill in this page again)

442496 A7 B7 printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (7) 1 Figure 2. Gas phase analysis of the hydrolyzed acetylation derivative of SACCHACHITIN SHIMADZU model 9A capillary Separation tube (80m X 0.22m · 60QC2 / ΕΡΙΟ, SGC, 5 AUSTRALIA) ° Injection: the temperature of JL is 250 ° C, the heating rate is 3 TV MIN from 120 ° C to 240 X: ° Use gas to carry the gas Zhao Nitrogen was used to provide FID detector gas and bone food. 10 3. Glucose 6. N-Acety lglucosamine 15 Figure 3. SACCHACHITIN belly (SACCHA-CHITIN) belly, after hydrolysis with 6N hydrochloric acid, Use ethyl acetate: isopropy (i sopropy 1 a 1 coho 1): water = 1: 1: 3 as the extractant (A) use naphtharesorcinol 20 trial leftover (B) or EIson-Morgen test agent is the developer, and then heated at 100 ° C for 3 minutes. 24 3. Glucose 9 (Please read the notes on the back before filling in this page)

This paper size applies to Chinese National Standard (CNS) A4 specification (2 丨 0X29 "? mm) 442496 A7 B7 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of invention Lu) 1 6.N-ethylglucosamine ( N-Acetylglucosamine) 8. oc -Amy 1 ase 9. Sugar and sugar || each (> 9 -G lucosidase) 10. weave noodles (Ce 11 u 1 ase) 5 11. carapace spleen (Chitinase) 12. Control group (Ccmtrol). Figure 4. Observation of the SACCHACHITIN membrane at different purification stages with an electron microscope. ◦ 10 (A) Ganoderma plant debris (B ) After inspection (C) The final product Figure 5. Photograph of wound healing process 15 (left) indicates the cotton in the back cover (right) indicates SACCHACHITIM in the back cover. On day 7 of (A) ( B) Day 14 (C) Day 21 20 (D) Day 28 (E) Normal skin after treatment Figure 6. Comparison of the degree of contraction of the wound 24 □ Use cotton 10 (please read the note on the back first) Please fill in this page for more details) This paper size is applicable to China National Standards (CNS > A4 size (210X 297mm)) 442496 Cooperative printed A7 B7 V. Description of the invention (9) 1 • BESCHITIN △ SACCHACHITIN The mycelium of the purification strategy type developed by the present invention or its basic method after its extraction In a five-pass method, take one thousand grams of fresh zeolites or their slag, pulverize them, and soak them in a second rotation for 48 hours, collect the residual method and dry them at 40 ° C. Dry the dried product with 1N sodium hydroxide at 85 t. It was digested for 24 hours. The remaining method was collected and rinsed with deionized water to remove the argon sodium in the residual method, and then decolorized with 0.1% hypochlorite, and then repeated with deionized water. 10. The length of the fiber is usually between 10 and 50 am. After collection, it is bubbled into a suspension with deionized water. Using a filter paper to press the suspension under the state of sinking before it is over-reduced, it can form a thin warm on the reduced paper. Named as sacchachitin 〇 After cold beam drying of Lay (EYELA, model FD-5N), split rod 15 and physical experiments were performed; chemical analysis was performed to determine that the SACCHACHITIN membrane contained sugar groups. Take 2 grams of SACCHACHITIN 蹊, crush and wash as you like To clean, use a sieve method to select 0.2 g of powder of the appropriate length in the pulp-like residue 20, and digest it with 1 ml of 12 N hydrochloric acid at room temperature for 4 hours. After the water price and hydrochloric acid were distilled off under a low pressure, the thin portion of the hydrolysate 'was hydrolyzed by TLC, and the rest of the hydrolysate was acetylated, and analyzed by gas phase chromatography (GC). 24 11 This paper size applies to Chinese National Standard (CNS) A4 (210X297 mm) (Please read the precautions on the back before filling this page}

442496 Printed by the Consumer Standards Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 V. Description of the invention 1) Analysis of the degraded hydrolysate of SACCHACHITIN using thin layer chromatography (TLC) is shown in Figure 1 (A and 8) It is frequently shown that the main ingredients of dialcecafil are N-ethylglucoseamine and glucose. The analysis of the hydrolysate of ethyl alcohol fermentation by gas chromatography (GC) is shown in Fig. 2 ', and its main composition is two cost. Comparative standard curve scale The ratio of the two components is 60.15 to 39.85. Method for analyzing the glycosyl structure: 0.2 grams of the crushed powder was soaked in a scale acid buffer solution (pH = 7.4, 0.1H), and this suspension was used by various | 1 ^ 1836), cellulose complex (〇6 11111336), sugar 4 # (/ 3 Glucosidase), Chitinase hydrolysis, the process of hydrolysis is to maintain different enzyme states at an appropriate temperature, the role 24 to 72 hours. In addition, the control group did not add any enzyme, and its hydrolysate was analyzed by the aforementioned thin-layer chromatography (TLC) conditions and compared with standard products. The test results were determined. The glycosyl composition was determined as shown in Fig. 3. Glycans produced after digestion. Because only chitinase and sugar scale (/ 3 -g) .ucosidase can hydrolyze SACCHACHITIN, its composition can be determined as N-Acetylglucosamine and _ Glucose (0 — glucose) 〇20 The suspension of polyglucosamine fiber fibers screened by deionized water soaking during the production process, using the Nelson method with bovine albumin as the standard And determine the composition of white matter, and determine the total nitrogen content by the method of 24 KJeldahl. The results found that the Seka record (12 ^^^ Applicable to the Chinese National Standard (CNS) A4 specification (21 ο X 297 Gong)) (Please read the notes on the back before filling in this card), \ vs

Printed by the Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs * 44249 6 A7 B7 V. Description of Invention (11) 1 SACCHACHITIN) has no color reaction, indicating that the Secchi juice does not contain any protein. Its atmosphere contains 3.46¾, which is equivalent to the wattage content of N-Acetylglucosamine < = to further determine the fine hypha of Ganoderma «also walled with polyglucose (/ 5 -giucan) 5 and R-Acetylglucosamine are the main components. 0 ′ Polyglucosamine weaves produced at different stages of Qin by electron microscopy, as shown in Figure 4, in the Ganoderma plant The tissue fragments contained thin daggers and irregularly distributed pores, and the fibers gradually smoothed after empirical treatment. The final pore size of SACCHACHITIN was 0.2-0.3 wm. To accentuate the abdominal structure of SACCHACHITIN, it can be used as a substitute for injured skin, as artificial skin, to help the healing of wounds: as well as to improve the shape of spines in the film: better than the physical properties of powder characteristic. In the study of 15 treatments for rabbit wounds, regardless of the degree of wound sore closure or the degree of constriction, the performance of Secchi:? T101 is comparable to that of Khan. The polyglucosamine fiber was treated with Acetaminophen as a model drug, which usually showed a split and widening when it was directly hit. According to the prescription of Table 1, the formulations were compared. Comparison of suspicious characteristics. The results are shown in Tables 2, 3, and 20. Table 4 clearly shows that the film contains the appropriate amount of benzene-based weaves and has the characteristics that can be suspicious. Polyglycine fibers of 50-200 yum size were screened for excipient 0, and washed in accordance with the ratio shown in Table 5 or Table 6. Polyglucide was woven according to the ratio, but less; eros (aeros i 1 * 24 2000), microcrystal 1 ine cellulose * MCC 13 This paper size is applicable to China National Standard (CNS) A4 specification (210X297 mm) (Please read the precautions on the back before filling this page)

Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs 442496 A7 __ B7 V. Description of the Invention (12) 1. Ethylamine is ready to dry. Test the repair status between the excipient and the drug, as shown in Table 7 and Table 8; The frequency shows that benzene powder can improve the physical properties of the powder bundle in the pound tablet no matter whether it is used as a dry medium with microcrystalline fiber or ethyl powder. 5 In order to make the censorship more advanced-further understand the present invention's following a courteous embodiment of the present invention, with a detailed explanation of the creation in conjunction with the enclosed round form, the description is as follows: 10 Real Example 1 SACCHACHITIN) Dirty buddha takes one thousand grams of fresh stupid or its submerged, crushed and run in acetonitrile for 48 hours, collect the residue and dry it under 40 ° C. The dried product was digested with 1N sodium hydroxide at δ5 < = c for 24 hours. The basic residue was collected and rinsed with deionized water to remove sodium hydroxide, and then decolorized with 0.1% hypochlorite, and then rinsed and washed with deionized water. The method selects fibers of appropriate length in the pulp-like residue. Polyglucosamine fibers with a length of 10 to 50 Wm are collected and suspended in deionized water to form a suspension. Under aseptic conditions, the suspension is filtered under pressure using filter paper to form a thin layer of warmth on the reduced paper. Example 2. Study on wound treatment 24 A Wistor rabbit (3 ---------------: 丁 —_____ 〆— (Please (Read the notes on the back before filling out this page) 14

442496 Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs A7 B7 V. Description of Invention (13) One month old and weighing 500g + 50g). First, rabbits were anesthetized with pento-barbital. Use an electric razor to remove the hair on the back of the rabbit's ears, and take 2 pieces of skin with a surface area of about 2.5x2.5 cm1 at a distance of 4.5 cm from the back of the ear and 1 cm from the ridge of the spine. Cotton is used as a control ancestor, and the right side is encyclopedia iT (SACCHACHITIN), and BESCHITIN produced by MORITIHITA RESERE is also compared. Then place the rabbit 'in an animal bottle of a 30 C air wash device. 10 The results are shown in the photos of the wound healing process in Figure 5, and Figure 6 plots the degree of contraction of the injured area against time, comparing different skin substitutes to help the wound flood and heal. In Figure 5, covering the wound with SACCHACHITIN has improved improvement compared to covering the wound with cotton and it has significant differences. The change in wound area is shown in Figure 6. Both SACCHACHITIN 15 15 and BESCHITIN assist wound healing and contraction. The gap is very small. In a 9 cm square injury, using any of the aforementioned products. After adequate treatment of 30 夭, the skin will show normal growth, and the hair on the back of JL will grow back after the wound is healed. Therefore, the treatment of wound healing or its effectiveness as a skin substitute is comparable to that of the 20th phase. Example 3 24 Polyglucosamine weaving screened in the process of making Buddha, hot air at 45 ° C 15 This paper size applies Chinese National Standard (CNS) A4 specification (2 丨 OX297 mm) (Please read the back first (Notes for filling in this page)

442496 Printed by the Shell Sample Consumer Cooperative of the Central Bureau of Procurement, Ministry of Economic Affairs A7 B7 V. Description of the invention (14) Dry under 1 to 5% w / w. Treat the fabric with room temperature water, add 25ml of water according to the ratio shown in Table 1 and the polyglucosamine fiber, Acetaminophen, 1% aerosi (2000) in groups A ~ A3, The wet mass is passed through a No. 16 sieve. After granulation, it is dried at 50 ° C for 3 hours and then passed through a No. 20 sieve for granulation. Wet washed with polyglucamine fiber and 30 ml of water, sieved, granulated and dried for 3 hours, then sieved and granulated as a control group. V. Practical travel example 4 10 Treat the fiber with 85 ° C water in the same way as in Example 3, add 25ml of water and mud according to the ratio shown in Table 1 to the group of B ~ B3. 1% aerosi (aerosi 1-2000), made into a full group and passed through a No. 16 sieve. After granulation, it was dried at 50 ^ C for 3 hours and then passed through a No. 20 sieve. Yuange block 15 containing only polyglucosamine weaving reed and 30ml of water was used. After sieving and granulating, we tried to sieving and granulating for 3 hours, then used as a control group. Example 5 The fiber was treated with 1N sodium hydroxide in the same manner as in Example 3. 25 ml of 20 water was added in accordance with the ratio shown in Table 1 to mix the C-C3 polyglucamine weave, beta a & amine (Acetaminophen), and 1%. Aerros (aeros i 1-2000), made into a full-bodied rose and passed through a No. 16 sieve. After granulation, it was dried at 50 ° C for 3 hours and then passed through a No. 20 sieve. Yuange 24 was washed, crushed, granulated and dried for 3 hours, then sieved and granulated as a control group. 16 This paper size applies to China National Standards (CNS.) A4 specification (210X297 mm) ____; I _ _, — I called (please read the precautions on the back before filling this page) 'Order 442496 ffff i] Supplement A7 B7 V. Description of the invention (15) I Example 6 The polyglucosamine fiber screened in the preparation process was 'smashed and sieved to retain 50-200 "m undersized weaving dimension' according to Table 5 The ratios of mud and poly 5 were amine · weaving, 1% aerosi (aerosi 1-2000), crystalline paper vitamin (MCC), and ethyl brewed amine pan and dried together. 0 Example 7 10 During the process of making Buddha The sieved polyglucosamine weaving fabric is sieved and sieved to protect the less than 50-200 um weaving fabric. According to the ratio shown in Table 6, Langhe Polysilicone weaving fabric, 1¾ aerosi (aerosi 1 -2000), Huanjing Cellulose (MCC), Ethylamine, drying together. Test the modification state of the excipient or drug, such as the characteristics of the powder; and the characteristics of the agent. 15 (Please read the precautions on the back first (Fill in this page again) Order-1 Printed by the Employees' Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs 20 24 17 This paper scale is applicable to the Chinese National Standards (CNS > Λ4 Regulations) Grid (210 X 297 mm) 44249 6 47 A / B7 V. Description of the invention (1β) Printed by the consumer cooperation of the Central Bureau of Standards of the Ministry of Economic Affairs

Aerosil-2000 Glucosamine Fiber Acetaminophen f! 1 1 Η-1 > ί— * Η- * 丨: 丨 Off: 丨 Η- * Name to H- * Η- * Rong u > Η- » U) 1 1 1 1 t— »:: ϊ ::: 丽:. Η- * 这 1—» t— »: 丨 :::::: 丨 | _ ts) 1—» W 1— * OJ 1 — * Is ί 1 1 H— * I— * Η- »1— * 彡 to — :: &&;. 一 容 〇J H- * Q 一 ----------- S ^ ------ Τ (Please read the notes on the back before filling out this page) r 8 This paper size applies to China National Standard (CNS) A4 (210X 297 mm) 442496 Printed by the Consumer Standards Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs System A7 ___ B7 V. Description of the invention (I?) Table 2 Recipes: Tolerance (ton) Thick skin (mm) Diameter (mm) Brittleness (%) Crush & degree A 0.5 ^ 6.30 (0.07) a 9.91 (0.09) 100.00 1.3 (0.1) 1.0 6.31 (0.06) 10.17 (0.07) 100.00 1.8 (0.2) 1.5 6.34 (0.03) 10.18 (0.05) 100.00 1.6 (0.2) 2.0 6.29 (0.05) 10.20 (0.05) 100.00 1.6 (0.2) 3.0 6.13 ( 0.04) 10.25 (0.03) 100.00 1.9 (0.3) A1 0.5 6.31 (0.06) 10.22 (0.25) 1.92 6.1 (0.4) 1.0 6.26 (0.04) 10.24 (0.01) 0.95 8.4 (0.9) 1.5 6 .10 (0.04) 10.22 (0.01) 0.98 11.1 (0.4) 2.0 6.04 (0.06) 10.23 (0.02) 0.75 12.8 (0.6) 3.0 5.97 (0.03) 10.21 (0.01) 0.65 13.5 (1.1) A2 0.5 6.50 (0.02) 10.25 ( 0.02) 2.88 5.6 (0.4) 1.0 6.36 (0.06) 10.25 (0.04) 1.30 8.6 (0.5) 1.5 6.25 (0.07) 10.24 (0.03) 0.96 11.2 (1.5) 2.0 6.12 (0.04) 10.23 (0.03) 0.75 12.3 (0.4) 3.0 6.05 (0.04) 10.21 (0.02) 0.77 15.1 (0.8) A3 0.5 6.59 (0.04) 10.25 (0.02) 3.67 5.4 (0.3) 1.0 6.41 (0.06) 10.24 (0.02) 1.34 9.1 (0.7) 1.5 6.28 (0.07) 10.26 (0.03) ) 0.96 11.6 (0.4) 2.0 6.17 (0.06) 10.24 (0.02) 0.73 11.6 (0.8) a, body 3.0 6.09 (0.07) 10.22 (0.01) 0.86 19.0 (0.8) Mean (SD) 19 (Please read the note on the back first Please fill in this page again for this matter) This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm) 442496 V. Description of invention (18) (ton) Thickness (mm) Diameter (mm) Crispy skin (%) Crushing degree (Kp) B 0.5 6.29 (0.07) a 9.87 (0.12) 100.00 1.3 (0.1) 1.0 6.21 (0.06) 10.07 (0.07) 100. 00 1.7 (0.1) 1.5 6.20 (0.05) 10.20 (0.05) 100.00 2.0 (0.2) 2.0 6.20 (0.05) 10.18 (0.12) 100.00 2.0 (0.4) 3.0 6.12 (0.07) 10.16 (0.12) 100.00 1.7 (0.2) B1 0.5 6.42 (0.06) 10.23 (0.01) 1.67 6.0 (0.4) 1.0 6.28 (0.06) 10.25 (0.03) 0.92 7.7 (0.4) 1.5 6.11 (0.08) 10.24 (0.08) 0.73 10.1 (0.9) 2.0 6.04 (0.06) 10.22 (0.03) 0.59 12.4 (1.4) 3.0 5.95 (0.02) 10.23 (0.04) 0.63 13.7 (0.6) B2 0.5 6.51 (0.02) 10.22 (0.01) 1.77 6.1 (0.6) 1.0 6.36 (0.03) 10.24 (0.01) 0.89 10.3 (1.5) 1.5 6.10 (0.04) 10.12 (0.06) 0.75 13.4 (0.8) 2.0 6.12 (0.06) 10.22 (0.01) 0.62 15.5 (0.8) 3.0 6.01 (0.03) 10.22 (0.01) 0.89 17.5 (0.8) B3 0.5 6.60 (0.04) 10.26 (0.02) 2.44 5.9 (0.5) 1.0 6.41 (0.03) 10.23 (0.02) 1.15 8.9 (1.0) 1.5 6.28 (0.07) 10.25 (0.03) 0.92 12.2 (0.3) 2.0 6.17 (0.08) 10.23 (0.02) 0.73 13.3 (1.4) 3.0 6.10 ( 0.07) 10.25 (0.03) 0.79 17.3 (0.8) a Mean (SD) 20 (Please read the notes on the back before filling out this page) The paper size is generally Chinese National Standard (CNS) A4 size (210x297 mm) 44249 6 A7 B7 V. Description of the invention (ig) Table 4 Fangyi match c Thick skin diameter Crispy skin crushed (mm) (mm) (%) _ (Μ. 1Lc c2 c3 1 · 1 · 2 · 3 · 0 · 1 · 1 · 2 · 3 · 0 · 1 ·! 2 · 3 o 1 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 6.35 (0.05) 3 10.25 (0.01) 0.52 8.0 (0.5) 6.32 (0.02) 10.27 (0.02) 0.54 7.9 (0.3) 6.30 (0.03) 10.27 (0.01) 0.27 6.6 (0.3) 6.16 (0.02) 10.28 (0.03) 0.00 8.7 (0.6) 6.09 (0.03) 10.24 (0.02) 0.08 9.2 (1.2) 6.40 (0.04) 10.24 ( 0.02) 1.63 6.2 (0.3) 6.22 (0.02) 10.22 (0.01) 0.96 8.9 (0.8) 6.07 (0.02) 10.23 (0.04) 0.81 11.2 (0.6) 6.04 (0.02) 10.22 (0.03) 0.55 12.7 (1.5) 5.93 (0.04) 10.09 (0.06) 0.62 14.7 (0.6) 6.56 (0.04) 10.23 (0.03) 2.23 5.7 (0.4) 6.38 (0.05) 10.23 (0.01) 0.96 9.3 (0.9) 6.20 (0.07) 10.24 (0.04) 0.82 12.7 (1.2) 6.14 ( 0.06) 10.24 (0.04) 0.59 14.9 (1.9) 6.03 (0.05) 10.22 (0.01) 0.70 15.8 (1.3) 6.63 (0.03) 10.24 (0.01) 1.75 6.3 (0.4) 6.42 (0.03) 10.24 (0.02) 0.82 10.6 (0.7) 6.26 (0.09) 10.23 (0.02) 0.73 14.3 (0.3) 6.08 (0.02) 10.21 (0.01) 0.62 16.0 (0.9) 6.00 (0.03) 10.23 (0.03) 0.67 20.3 (0.9) (Please read the notes on the back before filling this page)

,? T 〆 r

Mean (SD) 21 This paper size applies to China National Standards (CNS) A4 format (210X297 mm) 442496 A7 B7 printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (20) § η S 诹 mmm ttrv Thin g® 箓 谥 ntt · m 1 1 1 KJ \ η- »N) 1 1 CA · — * 〇1 1 H- * — w: 1 1 H- * H- *; k ': 1 1 s h- * m: N > 1 1 — ffi: .Ui: · 1 1 — 1 (— 1— »h *: 1 1 Η-» 1 1 — H- *: U >; 1 1 H- * · — »1 1 1—» 1— »1 1 t—1 — 1 1» — * Η- »s | η im 8〇 thank Bo Lishen rttp > Zashun rttv W 1 1 1 1 1 α 〇is) — Η- • ►— * ';' · 〇I / »— Η-» I— »Η-» α U »! 1— ^ t— :: 货! —: Tn: i; 1 1 Η-» Η -* m! — ►— *: 羽 ::! U) Η- * Iron r 1 Ui — Η-; Ϊ w Η- »'Κί Coffee 1 w 1— *. 匁 :: UJ 1 1 Η« *. 0 :: 1 1 t— * 0 — ^ 卩 〆. ^ 1- -I mmm. ........ I-^ inn-m I— 1Γ l · — 1Γ n ---- n .w -! (Please read the notes on the back before filling this page) Chinese National Standards (CNS> 8 4 specifications (210X29 * 7 mm) 442496 A7 B7 V. Description of the invention () Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs § MG g 0m Z2C3 m Rumor Sm m Consultation rttv m M USi mf 1 1 Private H-1 Ut r: 1 1 \ o LA r〇 曹 1 Private H- * v〇s: 〇J 1 1 to 1/1 K) L / \ Ό z 1 1 to K) La v〇 (y \ > w 1 1 NJ to La v〇Ui 2:. To 1 1 K) LA is) LA Ό L / \ ZU) 1 1 Η—1 Ό L / l 〇1 1 ^ Ό LA 〇1— · 1 1 Η- * O to 1 1 dry \ o KM c U)

1 I _ m ΐ ^ αα she m rtrtt > 逶 gni m ttty · 驸 (N: ϊ 圏. M βΜ-: ·: 1 t! 1 1 汔 ::: six: p! K > Η- »1 1 ί—1 p 容 H- »1 1 to η- *! ►—» 1 1 汔: β c-ϋ 1 i L / \ 1 1! 1 1 \ 〇'- ': f—f α eight f— * 1 i * 1 1 1 Exposure 1 1 ^ j: 1 1) — * VO KM 23 This paper size is applicable to China National Standard (CNS) A4 (210X297 mm) ---------- 1 -I (Please read the precautions on the back before filling this page) JT '* ~~ It --- i 44249 6 A7 B7 V. Description of the invention (22) Table 7 Formulation repose angle 〇 Sinking angle Loose density Tap density (° ) (g / 100ml) (g / 100ml) Card Index (%) Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs D 39.7 (1.2) 8 35.7 (2.1) 48.69 (0.71) 57.89 (0.30) 15.89 (0.86) Dlb 40.7 (1.6) 33.7 (1.5) 49.88 (0.42) 57.43 (0.50) 13.15 (0.56) D2 41.0 (0.5) 34.7 (0.6) 49.55 (0.84) 58.44 (0.82) 15.22 (0.82) D3 41.8 (1.3) 37.2 (1.9) 50.06 (0.41) 58.95 (0.72) 15.07 (0.35) E 41.2 (1.8) 37.5 (1.3) 47.63 (0.25) 56.02 (0.18) 14.98 (0.52) El 39.0 (2.8) 35.0 (3.0) 50.10 (0.12) 59.04 (0.70) 15.13 (1.19) E2 40.7 (2.1) 36.7 (0.6) 50.98 (0.36) 59.25 (0.15) 13.96 (0.45) E3 39.0 (1.7) 35.3 (1.5) 51.42 (0.20) 59.12 (0.24) 13.02 (0.51) F 41.2 (0.3 ) 30.5 (0.5) 44.47 (0.50) 58.49 (0.61) 23.97 (0.49) FI 42.0 (1.7) 27.7 (1.2) 48.68 (0.87) 64.42 (0.26) 24.43 (1.50) F2 42.2 (1.4) 34.0 (0.0) 48.87 (0.12 ) 64.42 (0.34) 24.13 (0.57) F3 41.7 (1.5) 31.0 (2.7) 49.48 (0.25) 64.58 (0.17) 23.38 (0.52) G 44.3 (1.5) 24.7 (1.2) 47.54 (1.74) 61.98 (1.45) 23.31 (1.85 ) G1 41.3 (2.5) 30.3 (2.5) 51.17 (0.74) 65.56 (0.33) 21.95 (1.32) G2 41.5 (1.3) 31.3 (1.2) 51.67 (0.22) 66.06 (0.17) 21.77 (0.42) G3 40.3 (1.2) 31.7 ( 1.5) 53.45 (0.47) 66.43 (0.45) 19.54 (0.97) H 41.7 (1.5) 30.7 (1.5) 41.30 (1.38) 51.71 (0.88) 20.12 (3.03) HI 41.7 (2.3) 32.7 (0.6) 43.32 (0.32) 54.28 ( 0.34) 20.19 (1.02) H2 42.3 (2.5) 35.7 (1.2) 45.63 (0.66) 54.50 (0.09) 16.27 (1.23) H3 38.7 (2.1) 33.7 (0.6) 45.09 (0.30) 54.68 (0.17) 17.54 (0.39) I 47.0 (3.5) 25.3 (5.9) 30.56 (1.57) 47.99 (0.58) 36.34 (2.48) II 45.3 (0.6) 27.0 (3.6) 37.16 (0.73) 52.39 (0.51) 29.09 (0.71) 12 42.0 (2.0) 25.3 (1.5) 39.50 (0.25) 53.16 (0.29) 25.69 (0.80) 13 43.0 (2.0) 26.3 (3.5) 39.69 (0.52) 53.85 (0.49) 26.28 (1.02) J 46.0 (2.7) 29.7 (3.5 ) 30.73 (0.37) 46.03 (0.44) 33.24 (0.24) J1 46.0 (0.0) 23.3 (2.3) 36.41 (0.39) 51.71 (0.26) 29.58 (0.52) J2 46.7 (1.5) 25.7 (2.9) 38.44 Π.01) 55.57 ( 0.58) 30.82 (2.36) J3 43.3 (2.5) 26.3 (2.1) 37.19 (0.20) 52.03 (0.31) 28.52 (0.61) Mean (SD); n = 3 codried powder with 1: 0.2%, 2: 0.5%, 3: 1.0% Aerosil-200 (Please read the precautions on the back before filling this page) Order i 24 This paper size applies to China National Standard (CNS) A4 specification (210X297 mm) 442496 A / B7 V. Description of invention (23) Table δ Formulation Repose Angle Sinking Angle Loose Density Percussion Pico Card Index 0 (°) (g / 100ml) (g / 100ml) (%) K 63.3 (2.1) 8 46.0 (1.7) J7.71 (0.57) 31.67 ( 1.99) 43.92 (4.54) Klb 59.0 (0.0) 44.0 (6.9) 17.48 (0.79) 31.22 (0.69) 44.03 (1.81) K2 61.0 (3.5) 36.7 (7.6) 20.20 (0.88) 38.09 (0.51) 46.98 (1.65) K3 57.7 (0.6) 36.3 (1.5) 23.30 (0.34) 36.33 (0.77) 35.83 (2.23) L 63.0 (0.0) 43.3 (1.5) 1 1.93 (0.74) 20.94 (2.06) 42.89 (2.99) LI 59.3 (1.5) 43.7 (0.6) 14.23 (0.24) 21.85 (0.60) 34.84 (2.71) L2 55.7 (0.6) 35.3 (3.8) 16.43 (1.30) 24.05 (1.01) 31.73 (2.83) L3 56.0 (1.0) 35.3 (2.1) 18.71 (0.32) 28.31 (0.37) 33.89 (1.96) M 66.0 (2.0) 42.3 (1.2) 13.09 (0.15) 21.56 (1.07) 39.18 (2.51) Ml 62.7 (1.5 ) 40.3 (2.5) 13.91 (0.66) 21.78 (0.35) 36.16 (2.63) M2 57.7 (2.3) 40.3 (5.5) 17.06 (0.05) 24.64 (0.81) 30.69 (2.38) M3 55.7 (2.1) 36.7 (1.5) 20.21 (0.22 ) 28.97 (0.68) 30.18 (2.30) Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs N 58.3 (1.5) 44.3 (2.3) N1 59.0 (0.0) 43.3 (4.5) N2 57.3 (0.6) 35.7 (1.5) N3 56.3 (1.5 ) 41.7 (1.5) 0 62.7 (0.6) 46.0 (3.0) 01 58.3 (0.6) 37.3 (2.5) 02 59.3 (1.2) 39.7 (4.7) 03 54.7 (3.1) 40.0 (4.4) 3 Mean (SD); n = 3 b codried powder with 1; 0.2 15.30 (0.70) 25.04 (1.68) 38.62 (6.67) 14.93 (0.82) 22.46 (1.05) 33.48 (3.78) 15.60 (0.25) 25.08 (1.03) 37.74 (2.50) 20.59 (1.09) 30.67 (0.58 ) 32.89 (2.64) 16.65 (0.39) 30.75 (1.18) 45.78 (2.62) 17.28 (0.20) 29.74 (0.76) 41.89 (0.86) 20.14 (0.41) 32.46 (0 .32) 37.95 (0.78) 23.98 (0.31) 36.02 (0,49) 33.41 (1.41)%, 2: 0.5%, 3: 1.0% AerosiI-200 25 This paper applies the Chinese National Standard (CNS) Α4 wash scale (210X297 mm) (Please read the notes on the back before filling this page)

442496 A7 B7 Recipe Force (ton) Thickness (mm) Diameter! (mm) Brittleness (%) Breaking strength (Kp)

E

F

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J ^ 0500505005050500505005050050500 _ · * · ♦ ·· «♦ · ♦ ··· ♦ ····· ♦ 11 11 2 3 ο IX 1 2 3 ο 1 n 2 3 ο 1 1A 2 3 ο 1 1 2 3 ο 1 1 2 3

Mean (SD) 4.13 (0.05) 7.76 (0.02) 3.96 (0.03) 7.66 (0.03) 4.00 (0.03) 7.68 (0.01) 3.96 (0.04) 7.66 (0.04) 3.96 (0.04) 7.65 (0.05) 3.83 (0.18) 7.62 ( 0.08) 3.64 (0.05) 7.60 (0.04) 3.58 (0.11) 7.60 (0.03) 3.61 (0.02) 7.59 (0.03) 3.62 (0.02) 7.62 (0.03) 3.72 (0.10) 7.62 (0.05) 3.64 (0.07) 7.61 (0.04) 3.61 (0.04) 7.60 (0.04) 3.60 (0.01) 7.69 (0.03) 3.54 (0.01) 7.58 (0.02) 3.67 (0.18) 7.61 (0.05) 3.60 (0.04) 7.62 (0.02) 3.55 (0.00) 7.57 (0.04) 3.56 ( 0.03) 7.66 (0.030 3.52 (0.05) 7.62 (0.03) 3.54 (0.03) 7.55 (0.04) 3.56 (0.04) 7.63 (0.02) 3.50 (0.02) 7.60 (0.02) 3.47 (0.03) 7.64 (0.03) 3.44 (0.02) 7.59 (0.03) 3.56 (0.05) 7.60 (0.02) 3.53 (0.02) 7.59 (0.02) 3.51 (0.01) 7.56 (0.03) 3.51 (0.02) 7.60 (0.02) 3.44 (0.01) 7.56 (0.02) 26 0 0 (Γ 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6.6 (0.6) 9.3 (1.2) 7.5 (1.1) 8.4 (1.8) 8.9 (2.2) 13.8 (1.4) 21.6 (3.1) 20.8 (2.8 ) 23.3 (2.4) 22.9 (3.1) 20.9 (2.3) 20.0 (3.4) 22.3 (3.4) 23.9 (2.4) 26.1 (2.0) 21.8 (4.5) 23.2 (1.9) 28.0 (3.0) 25.1 (3.3) 25.2 (3.7) 27.3 (3.8) 28.3 (3. 1) > 35.0 > 35.0 > 35.0 30.4 (3.3) > 35.0 > 35.0 > 35.0 > 35.0

Greed ί ¥ S- Copybook I

Y Ding 'This paper size is applicable to China National Standards (CNS) A4 (210X297 mm) 442496 A7 B7 V. Description of invention (25) Table 10 Printing force for printing formula of the central government bureau of the Ministry of Economic Affairs Consumer Cooperatives 1 torce (ton) Thickness (mm) Diameter (mm) Brittleness (%) Fragmentation (Kp) L 0.5 4.32 (0.02) 3 7.59. (0.01) 0.11 12.8 (0.9) 1.0 4.10 (0.06) 7.58 (0.01) 0.12 16.2 (4.2) 1.5 4.08 (0.01) 7.59 (0.01) 0.16 20.5 (2.7) 2.0 4.05 (0.02) 7.59 (0.01) 0.15 19.6 (2.2) 3.0 4.02 (0.01) 7.60 (0.01) 0.18 6.8 (0.7) M 0.5 4.35 (0.03 ) 7.60 (0.02) 0.21 10.8 (1.9) 1.0 4.16 (0.02) 7.59 (0.01) 0.25 14.8 (2.3) 1.5 4.08 (0.02) 7.62 (0.03) 0.16 15.5 (3.0) 2.0 4.04 (0.02) 7.59 (0.01) 0.16 16.5 ( 1.8) 3.0 4.04 (0.03) 7.59 (0.00) 0.22 5.3 (2.0) Ν 0.5 4.31 (0.02) 7.59 (0.01) 0.21 12.9 (1.5) 1.0 4.17 (0.03) 7.60 (0.01) 0.24 14.4 (2.5) 1.5 4.08 (0.04) 7.60 (0.00) 0.24 15.0 (3.5) 2.0 4.03 (0.03) 7.60 (0.01) 0.14 14.5 (2.9) 3.0 4.02 (0.02) 7.58 (0.01) 0.35b 6.3 (1.2) 〇0.5 4.27 (0.03) 7.59 (0.01) 0.25 11.1 (2.6) 1. 0 4.16 (0.03) 7.62 (0.03) 0.44b 12.7 (3.0) 1.5 4.04 (0.03) 7.60 (0.02) 0.70c 17.1 (1.9) 2.0 4.01 (0.03) 7.61 (0.01) 0.40c 9.4 (0.9) 3.0 3.99 (0.01) 7.59 (0.01) 0.72 ° 3.4 (2.0) Mean (SD,) one tablet divided into two parts some tablets divided into several parts 27 This paper size is in accordance with China National Standard (CNS > A4 size (210X297 mm)) (Please Read the notes on the back before filling in this I) ϊ. Γ __I ____________ W ------ ^

Claims (1)

442496 A8 B8 C8 D8 The Central Standards Bureau of the Ministry of Economic Affairs, Consumer Cooperatives, India Poly 6. Application for patents. 1. A polyglucosamine fiber with a ratio of 60:40 (1-3) polyglucose (zhao- (1-3) -D-Glucan) and N-Acetyigiucosamine co-polymerized fibers, and freshly crushed (Ganoderraa) plants or their residues are immersed in an organic solvent, dried, digested with sodium hydroxide, and used. Hypochlorite was decolorized, washed, and sieved fibers of appropriate length. 2. For the polyglucosamine fiber under the scope of patent application, the length of the fiber is usually between 10 and 50 / zm 〇3.-A kind of skin substitute, which will be like the towel in the scope of patent scope _Amine fiber is dried to make a porous film sheet. 4. For example, the amidine fiber of Shenqing Patent Fan @ 1 item can be made into pharmaceutical excipients after drying or crushing. 5. For example, polyglucamine and weaving in the scope of patent application No. 4 can be used as pharmaceutical excipients in various pharmaceutical dosage forms. 6. For example, the polyglucosamine fiber in item 1 of the patent scope of application μ can be added to the formulation of cosmetic preparations after drying or crushing to achieve the curative effect. (Please read the notes on the back before filling this page)-Order-Thread This paper size is applicable to China Standards (CNS) A4 «l grid (210X297 mm >