TW385229B - Insecticidal matrix and process for preparation thereof - Google Patents

Abstract

This invention relates to novel biopesticidal compositions comprising an active insecticidal ingredient selected from insecticidal bacteria and viruses such as B. thuringiensis crystal protein or spores or mixtures thereof and baculoviruses such as nuclear polyhedrosis viruses, granulosis viruses and non-occluded viruses; a polymer; and an inorganic light blocking agent wherein the light blocking agent protects the active ingredient from both ultraviolet light and sunlight and the polymer forms a matrix in which the active ingredient and light blocking agent are dispersed therein. Methods for producing the biopesticidal compositions and methods of controlling insects are also included within the scope of the invention.

Description

A 7 —,, B7 V. Description of the invention (丨) The background of the present invention for toxic side effects or the lack of specificity of certain chemical pesticides has led to the development of biological pesticides, especially for insecticidal bacteria and Viral aspects. Generally speaking, this species of Bacci 11 usthuringiensis has become the most successful biological control agent of all microorganisms, and the strain K of Sulindac has the same attitude to chemical control agents. It has been regarded as a kind of Insecticide. Among the naturally occurring bacillus protein toxins is a polypeptide crystallizer. These crystalline toxins are active against many Kun * s, including lepidopteran insects (especially • Kostaky dysentery of S. sclerotiorum and B. t hur i ng i en sis var kurstaki and B. thuringiensis var. aizawai)); Coleoptera (except, S. thuringiensis var. tenebrionis); Μ and Diptera Kunft (especially Printed by the Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs of the Suli bacteria (read the first note on the reverse side and fill in this one hundred) (M. thuringiensis var. Israelensis). In addition, baculoviruses such as Heliothis nuclear polyhedros is virus (NPV) are particularly effective against Heliothis spp. Autographa cal if ornica nuclear polyhedrosis vivus (NPV) is effective against the anti-alfalfa and Trichoplusia ni (Kun S) class * and is used in biodeodorants in. Biocides are typically described explicitly as chemical pesticides, and are often applied via existing irrigation technology plus K. These preparations can be printed by the National Standard (CNS) Λ4 specification (210V, 297 g; t) of this paper standard, printed by the Bayer Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs, B7 _V. Description of the invention (ν) Insect pathogens ( Viruses such as NPV or bacteria 'such as selenium) • Spores and / or crystallized endotoxins (crysta 11 inede 11 a endotoxins). However, when used in this field, these blends have many disadvantages. When the biopesticide is exposed to sunlight and ultraviolet radiation, this effect can cause the inactivation of endotoxin-containing polypeptide *. At the same time, exposure to nucleic acids that can destroy spores makes the pesticide less effective. References have been published in Environmental Entomology by Ignoff et al., P. 6 411-415 (1 9 7 7) (Ignoffo eta 1. Environ. Entoiao 1., 6: 4 1 1-4 1 5 (1 9 7 7)). The reduced efficiency of this formulation makes repeated application necessary for susceptible vegetables that need to control insect production areas. A further disadvantage of some biocides is that, especially at low doses of Bacillus biocides, they are inhibitory eaters, but they are not lethal. Kun is a lethal ingestion that suppresses feeding doses * Stops feeding Can be read for a period of up to several days. This characteristic, when combined with the biocidal effect caused by thin sunlight (Xiang (inactive properties) can cause poor control of the eyesight. It has been tried to solve the unstable sunlight by providing a biocide formulation. The problem is that the killing bacteria and viruses and sunlight screening agents are included in this formulation. These formulations each include two encapsulated and non-encapsulated active toxins, but they all contain many disadvantages. Some disadvantages Specific to each compound. Common examples of capsule-encapsulated mixtures include: the polymer that forms the capsule wall does not always maintain the sunlight screening surface inside the capsule; in some examples ， Highly toxic substances are used in this paper. Applicable to Chinese national standard: CNS） Α4 size (210 × 2 () 7 mm) (Please read the precautions on the back before filling this page) " 装, 1Τ- Ϊ! A7 B7 V. Description of the invention (>)

Please I first read _ | read the% of the back I meaning I matter I • Xiang Guang I and then fill in I〗 I write. Chu buy I in addition to the preparation of some capsules; Made in a manner sufficient to provide any practical barrier to light sieve embedding (very small particles such as those formed by a two-phase liquid emulsion that actually penetrates its interior through consistent sunlight); and capsules are easily coated in Broken in the realm. Disadvantages with non-encapsulated mixtures include that most of the daylight screening agents do not normally come close enough to biological entities or toxins M to produce effects after being applied to crops or eye locations via splashing equipment; non-encapsulated mixtures are also prone to Suffering from the disadvantages mentioned above, Kuna ingestion was the most lethal inhibitory ingestion agent but later resumed their normal ingestion. Order-, the Ministry of Economic Affairs ordered the Central Bureau of Commerce to staff consumption cooperation Du printed equipment The present invention overcomes the shortcomings of the previous art composition and ingredients. The active proteotoxin should be encapsulated or encapsulated in a polymer matrix where the particles are large enough to contain a lethal dose of medaka. The encapsulation method is effective so that the major part of the spores and / or crystal salamanders containing the active toxin or ingredient is encapsulated and a small portion of the spores and crystal grades remain unencapsulated. The components and methods of the adult product will activate the active ingredients of the biocide or leave a residue of poisonous plutonium. Encapsulated capsules are definite in both the application and the environment. The active ingredient is released in the susceptible Kun Bang sale when Kun Kun is ingested. This composition contains light that is actually toxic to the egg, impedes the cocoon, and remains in the composition matrix after the pupae are applied between and after the target site. SUMMARY OF THE INVENTION The biocidal fungicide-kunyan effective composition, and the method for preparing the aforementioned composition, have been dried as a protection site from Kunxin pests. This biocidal noodle is made of a mixture of active ingredients that kills the bacteria or disease of Kunming. The paper size is applicable to Chinese National Standard (CNS) A4 specifications (210XW7 mm) A 7 ·. ______B7 _ V. Description of the invention (4 ) Such as bouillon or insect-killing crystal protein or a combination of cyanobacteria or baculovirus in spores and a polymer and an inorganic light blocking agent, where the polymer and榇 Photo-media hindering agents combine to form a base 霣 as an active ingredient. Therefore, 'In the first aspect, the present invention relates to a biocide-killing effective composition of a biopesticide, including: (a)-an active ingredient selected from a collection of kunmicid bacteria and viruses; ( b) a polymer, which is an aqueous vehicle that is soluble under neutral to alkaline conditions and insoluble under weak acid conditions, where the polymer forms a matrix; and (c)榇 Light blocking agent, wherein the aforementioned light blocking agent is insoluble in water * and diffuses with the active ingredient in the matrix formed by the polymer of (b), and wherein the light blocking agent protects the active ingredient from ultraviolet light and The sunlight is not activated. In a concrete example, the bacteria are bacillus and the virus is baculovirus. Printed by the Consumer Cooperatives of the Central Bureau of Standards, Ministry of Economic Affairs {Please read the notes on the back before filling this page) The composition thus formed can be further formulated into the final product of aqueous, dry or non-aqueous solutions. In a second aspect, the present invention relates to a method for preparing an effective insecticidal product of a biocide, comprising: (a) forming a polymer solution; wherein the aforementioned polymer is neutral in the form of an aqueous medium It is soluble under the test condition, and applies the Chinese national standard rate (CNS > A4 specification (2! OX 297 mm) A7 B7 in the weakly acidic paper size) 5. Inventive (<) is not possible (B) the formation of an inorganic light blocking agent; please read the precautions first, and then (c) obtain a suspension culture of insecticidal ingredients selected from the collection of bacteria and baculovirus (D) preparing a solution of a volatile matrix and combining the aforementioned solution with the suspension culture of step (c); (e) mixing the light to prevent the expansion and the polymer solution M to obtain a second diffusion, and The second diffusion is combined with a suspension of the active ingredient and a solution of volatile rhenium later; the mixture of M and (f) rhenium perforated (e >) wherein the active ingredient and the light blocking agent are diffused in a thin polymer In the quality Another specific example of the present invention includes a method for preparing an effective composition for killing quincetin that is a biocide. It comprises: (a) obtaining a suspension culture of an active ingredient, which includes selenium quincetin protein Or spores or related mixtures or stalk-shaped virus M and mixed with light that does not hinder cocoons and suspensions; printed by the Shellfish Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs (b) forming a polymer solution, wherein The aqueous vehicle type is soluble in neutral to sickle conditions and insoluble in weakly acidic conditions, where the polymer is a carboxy polyacrylic acid or styrene cis-butene containing about 3 to 10% of the composition The copolymer of dianhydride (c) is mixed with the mixture of (a) and the solution of (b) to obtain a suspension; the paper size is in accordance with the Chinese National Standard (CNS) Λ4 (2! 0'X mm)

Printed by the Consumer Standards Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs IA 7 B7 ___ V. Description of the invention (V) and (d) Leak-drying suspensions • The active ingredients and light barrier agents are expanded to the base formed by the polymer Langzhong. In another aspect, the present invention includes a method in an area where Kundun pests are expected to be controlled, including applying the disclosed composition of an effective insecticidal barrier to the aforementioned area. The present invention resides in the two aforementioned methods and the compositions formed thereby. Attach a brief description of circle_circle 1 indicates the ratio of the EC 50 activity to a composition, as described in Example 3 compared with the control sample. Detailed description of the present invention The biocide composition of the present invention is composed of three main components, an active insecticidal component, an inorganic photoinhibitor, and a polymer component forming a matrix of the composition. In a preferred example of purchase, the active ingredients are bacillus-killing bacilli, such as S. thuringiensis * B. pop illae and B. sphaericus, especially S. sclerotiorum . It is better to have quinclonal crystalline proteins and spores isolated from any naturally occurring S. thuringiensis subspecies or by DNA technology. The classification of thuringiensis is based on biochemical and hematological criteria. These classifications correspond to subspecies or variants with specific phenotypic characteristics. The subspecies of thuringiensis include thuringiensis and kurstaki -8-The standard of this paper is applicable to China National Standards (CNJS) A · »Specifications (2: v) 'xi9 ·? Pupae): ;-(Please read the notes on the back before filling this page) Order B7 _ V. Description of the invention (1), sotto subtype (sotto) · pine «subspecies (dendrolimus), kill S subspecies (ent 〇 m 〇cidus) · Aizawai, Morrisioni, Tolwortlni, M-Isroelensis, and others as disclosed in Badek and Fleiken (Η De B arjacand E. Frach ο η), Classification of B. thuringiensis strains (35: 233 (1990)). During the formation of spores, the thuringiensis synthesizes many of the most plum-like crystals of one or more bactericidal proteins. When the spores become hot, some crystals are released into the environment. The genes for crystalline proteins are known • and identified as Cry genes. Many Cry genes have been isolated, and it has also been determined that the proteins corresponding to these genes are different from each other in terms of sex. In commercial applications, the preferred strains of S. thuringiensis can show four or more crystalline proteins, and the crystalline protein isolated from S. thuringiensis may include more than one crystalline toxin protein. Printed by the Bureau of Standards Consumer Cooperatives The present invention may include any crystalline protein of S. thuringiensis corresponding to different genes, such as those listed and shown in Yaeauoto and Powell (T. Yaaanoto and G. Powe 11) in higher engineering The -1 in S agent (Advance Engineer Pesticides) (ed. Leo Kim) (1993) is by the way incorporated by reference. The invention also includes active toxins which are designed to be tritium. For example, a strain of S. thuringiensis that normally exhibited a high level of activity against lepidoptera could be designed to exhibit a CryIIIA gene of coleoptera activity. The advantage of appearance is that any paper scale of Bacillus active coat is applicable to Chinese National Standard (CNS) Λ4 specification (2 丨 0 '< 297 public net). Employees' cooperation with Central Standards Bureau of the Ministry of Economic Affairs · Du Yinben M-_Β7 ____ 5 'Explanation of the invention (9) Toxins can be used in the present invention. However, when the invention is not limited in any way, the preferred toxic proteins include CryIA · CrylB, CrylC · CrylD · CrylE, CrylF, CrylG, Cryll, Cryl 11, CryIV, CrylH, CryV, CryA, CryB, And any other malting compounds, mixtures or parts of them. Particularly preferred toxins include CrylC · CrylA (a), CrylA (b), CrylA (c), CrylE, CryIIA, and variants. • Mixtures or fractions of this type. Recombination technology has been used in this technology. Are well known * and include conjugation (c ο njugati ο η) and electroporation infusion (electroporation) M increase the number of toxin peptone produced by a particular Bacillus strain and design as a means to create a fusion or hybrid protein gene Application of protein. An example of a hybrid gene is Gz7. It contains different Cry peptone fragments and specific CrylE and CrylC proteins. This peptone is further described in the biotechnology of Bosch et al. (Bosch et al., Biotechnology 12: 915-918 (1994)), which is also incorporated herein by reference. Those skilled in the art know that other hybrid genes and the above examples are not intended to limit the present invention in any way. In addition to the insecticidal crystalline proteins made by different strains of K. thuringiensis, spores produced by the aforementioned strains can also have killing properties. For example, it is generally known that certain spores are effective against some quinces such as Galleria nellonella. In another preferred example, the active ingredient is baculovirus * rod-10- This paper is sized for China National Standard (CNS) A4 (210, mm), 1-III—I c (please first Read the notes on the back and fill in this page) Order • m K ^ — m · Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs A7 'B7 V. Description of the invention (?) Viruses are a large group of viruses that are divided by sugar There are three subgroups: nucleated polygonal virus (NPV); granulovirus (GV) and polydmavirdae. These viruses infect many of the most important morning crops of the Kunming Pest. One of the benefits of baculovirus is their host specificity. The most widely described tadpole baculovirus is the Autographa californica nuclear polygonal «virus (NPV). The virus's emblem is formed by virus closure in the nucleolus of infected host cells. The size of the closed «varies according to the particular subgroup. A closed body is a crystalline protein construct * in which viral particles are contained. In the present invention, the preferred nuclear polyhedrosis virus is alfalfa silverworm polyhedrosis polyhedrosis virus, Heliothis zea NPV (polypeptone virus, Heliothis zea NPV), Spodoptera exigua NPV, And a strain of Anangrapha facifera. Recombinant strains of S. thuringiensis of the nuclear polyhedrosis virus as mentioned above are also included in the scope of the present invention. References can be found in Biology of Nuclear Polyhedrosis, Volume 1 and Volume 2 (The Biology of Baculovinoes, Vo 1 I and II, Granados and Federici ecls.m, CRC Press, B oca R a < on , FL 1 9 8 6)-軎, as a review of nuclear polyhedrosis virus. The pesticidally active ingredient will comprise a composition weight ratio of about 10 to about 90, preferably a composition weight ratio of about 30 to 80 ¾, and preferably a composition ratio of about 45 to 75%. -1 1-This paper size is in accordance with Chinese National Standard (CNS) A4 specification (210X: »7mm) (Please read the precautions on the back before filling out this page)-Binding and ordering Λ A7 __B7__ V. Description of the invention U 〇 A polymer is generally defined as a combination of monomer molecules in a linear linear or M branched manner or a two-pronged manner. M is chemically bonded to form multiple overlaps. The polymer may be formed from a single type of monomers, or one or more types may be used. In the present invention, preferred polymers are polyvinyl acetate; poly (acrylic acid) or carboxylic acid derivatives of the same or similar type; shellac; and styrene and fluorene-butadiene anhydride copolymers and phases M derivative; and a mixture of phase M. More preferred are high molecular weight polymers and copolymers * M and most preferred are styrene / cis-butadiene anhydride copolymers and hydroxypolyacrylic acid. Printed by the Consumer Standards Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs (please read Caution 1 on the back before filling this page) The polymer that forms the matrix of the composition is defined by the following general characteristics plus M. The polymer must be able to maintain light blocking agents. This polymer must be insoluble in the environment in which it is processed, but it must be soluble in the environment of the sex side, usually in Kunyujia. Furthermore, if the carrier is liquid, the polymer should be insoluble in the formulated carrier. When water is used as a carrier in spraying tanks and capsules, the polymer should actually be insoluble. The matrix formed by the polymer should be sufficiently non-brittle so that it can withstand the abrasion and mechanical forces experienced by the composition or final product during processing and processing. The acid value of the polymer should be in the range of about 3.5 to about 9.0, preferably from about 5.0 to about 8.0, and most preferably from 5.5 to 7.0. Neutral to sickle conditions are defined as about sickle value of 6.0 to about sickle value of 11.5, preferably from about acid I »value of about 6.5 to about 11.0. Weak acid condition is defined as about acid _ value 6.0 to about acid sickle value 4.0 · and preferably from about acid value 5.5 -12- This paper size applies Chinese National Standard (CNS) A4 (210X 297 public health) Central Standard of the Ministry of Economic Affairs Printed by the Consumer Cooperative of the Bureau B7 _ V. Description of the invention (i () to about acid period value 4.0. Aqueous medium is one in which water is the solvent. Generally speaking * polymer will contain about 0.5 to about 25% The weight ratio is preferably from about 1 to about 15% by weight of the composition, and most preferably from about 3 to 10% by weight of the composition. The phosphor-free mounting inhibitor used in the composition of the present invention is Those that can not only inhibit ultraviolet radiation, but also K can suppress sunlight, but also include optical absorbers and optical reflectors. The optical media blocking agent must be compatible with polymer substances and active killing ingredients. Prior to ingestion, M expects the adult to be insoluble in any environment handled by the adult. It has generally been found that the biodetermination of UV radiation is not a good predictor of the stability of sunlight. Certain light barriers Agent can produce ultraviolet light in a mercury vapor lamp Performance is good, but it does not provide any protection for solar radiation. Further * In order to be useful and practical, the amount of optical media inhibitor should be functional in an amount that can be actually added to the formulation. It will not be powerful Substances that absorb ultraviolet or sunlight should generally be used in a very large amount, and therefore any particles coming from this substance should generally be large enough to achieve actual protection from sunlight or ultraviolet light. Generally clay, metal oxides and Rhenium-free minerals and water-insoluble dyes can be used as light blocking agents in the present invention. Two specific types of photo media blocking agents are particularly useful in the present invention. * Some include titanium dioxide. Optical reflector and carbon black, an optical absorber. In addition, melanin and rhodopsin can be used as light blocking agents. A combination of these agents can be added to the composition of the present invention. -13- This paper is compatible with the Chinese National Standard (CNS) Λ4 specification (210/297 mm) " Tr 'i Pack ---- (Please read the precautions on the back before filling this page) --Order one --- -. II11 ill A7 _ B7__ of the Consumers' Cooperative of Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (j〆) The light blocking agent contains about 5 to about 90% by weight of the composition, preferably about 5 to about 60 %, And most preferably about 10 to 50% by weight of the composition. Better compositions according to the present invention comprise from about 30 to 80% of active ingredient, from about 1 to about 15% of polymer, and from about 5 to about 60% inorganic light blocker. The percentage is the weight ratio of the composition. As used herein, "based on J represents a solid state composed of a polymer molecule of the present invention and the position occupied by other components containing the composition. , Premises or space. At least about 60% of the active ingredient in the polymer matrix, and preferably 60%, must be maintained. The term “diffusion” is used here to mean that it is mixed very evenly, so that the particles of light blocking or active ingredients are actually completely uniformly dispersed. They can be dispersed in water or polymer solutions in the manner described herein. Going further • The particles in the suspension are not themselves formed by a large collection of smaller particles. For this reason, large aggregates are considered to be particles having a diameter of more than 5 to about 30 microns, and preferably about 5 to about 20 microns in diameter. In a preferred example, the composition contains a chlorinated active protein from spores, carboxyl polyacrylic acid polymer or styrene-butadiene An acid anhydride copolymer or a mixture of K, and carbon black, which is a light-blocking hafnium. The active ingredient can be a naturally-occurring Thuringiensis strain or a group of Thuringiensis strains that exhibit an exogenous crystalline control toxin gene. Another preferred specific example includes an active ingredient selected from the Sulzer Kotsky variety. Further specific examples -14- The paper size is suitable for China's family ladder standard (CNS) Α4 size (210 y 297 mm) (Please read the precautions on the back before filling out this page). _ Order A7 B7 V. Description of the invention (\ Xi) Includes the tacky ingredients selected from the Takizawa mutans strain of S. sclerotiorum. However, another further specific example includes an active toxin selected from the group consisting of CrylC, CrylA (a), CrylA (b), CryllA, CrylA (c), and a mixture of fragments and phase contrast, including hybrid proteins. . The biocidal composition of the present invention can be prepared by adding M in the following general description. Printed by the Consumers' Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs (please read the precautions on the back before filling this page). The killing ingredients of Sulin bacteria can be prepared by methods widely known in this technology. But it is usually used for this composition The peptone and spores will be produced in the fermentation method. The fermentation method has been known and has been described by Berhard and Entusto in the environment, a biocide, Sully (Bernharc 1 and Utz in Evtwistle eta 1., Bauillus thuringiensis, an Environmental Biopesticide, Wiley and sons, (1 9 9 3)) and has been included in the reference. Thuringiensis strains with genes expressing active toxins are generally fermented or reproduced in a nutrient medium containing nitrogen sources, carbon sources, and mineral radon and adjusted to an acid Mt value of about 7.0. In this way, the effect is conveniently achieved by culturing from 24 to 72 hours at a temperature of about 20 degrees Celsius to about 40 degrees Celsius. Concentrations of suspensions of S. thuringiensis strains can be obtained by evaporating and / or centrifuging the yeast solution to the desired level. Thuringia suspensions typically contain about 2 to 10% pupal amount of insecticidal crystalline peptone and similar amounts of spores. Other additives that can be added to the thuringiensis suspension include, for example, volatile groups such as ammonium hydroxide and non-volatile acids such as sulfuric acid. In a preferred example, an ammonium tellurate solution is prepared and suspended with the active ingredient. 1-This paper is sized to the Chinese National Standard (CNS) A4 specification (210_X297 mm). Employees' Cooperatives, Central Bureau of Standards, Ministry of Economic Affairs. Neem A 7 B7 V. Description of the invention (\ +) Floating liquid mixing. The nuclear polyhedrosis virus component of the present invention can be obtained by multiplying K and multiplying by the widely known method in this technology, and reference is made to Anderson et al., Pr oc. IV IFS: Today ’s Fermentation Technology (Anderson et al. al ·, Proc. IV IFS: Feraent. Techno 1. today, 6 2 3- 6 2 8, 1 9 7 2) »and Austrian science and technology by Chakraborty and others (Chakraborty, S. et al., Australas . Biotechnol., 5 (2), 82-86, (1995)) Both of K's articles have been incorporated by reference. The general method involves cultivating young crickets that are susceptible to a particular nuclear polyhedrosis virus > and infecting the young crickets with a closed body of nuclear polyhedrosis virus. In the process of nuclear polymorphism, the urinary pulp of the young hips of the insect was homogenized. Another general method is to multiply Kunyu cells in a cell culture container containing nutrient solution. The virus of this container K-nucleopolygonal II virus was inoculated and cultured. After nuclear polyhedrosis virus infection has passed for some period of time, the contents of the container are collected, and nuclear polyhedrosis virus is recovered by centrifugation or centrifugation. The polymer solution is made from about 0.5 to about 20% by weight of the polymer in water > raise the water temperature to about 6 ° C to about 80 ° C, preferably to about 80 ° C 70 degrees Celsius, shake slightly and add water-soluble hydrazone, preferably ammonium argon or argon soda for a period of time, so that the final acid value is about 5.5 to about 8.0, preferably about 7 to about 8. This mixture is left to stir for a few hours until the night, and M ensures that the polymer is completely dissolved. All mixtures and expansion solutions are preferably achieved using a high tension mixer. -1 6- This paper size is in accordance with Chinese National Standard (CNS) Α4 size (210 × 297 male; t)---, ¾ .. Shi Zhi H si I / Γ. (Please read the precautions on the back before filling this page)

I n I ------· B7 V. Description of the invention (ί <) The acid test value can be achieved by adding μ in different methods, including mixing an appropriate amount of volatile reactors, such as water-soluble ammonia and non-volatile Acids, such as: Poor acid in polymer solution. The choice of acid susceptibility conditions is controlled by the need to maintain the polymer in solution prior to drying, and the need for sufficient volatile hydroxyl groups to evaporate during drying. The box is composed of a combination of a non-volatile acid and a volatile base in the suspension to be sprayed. The active ingredients in the polymer base and the light are hindered. The final particles have an acid age value when resuspended in water. Its acid ore value is about 0.5 to about 2.5 acid Mt positions lower than the former spray-lane suspension, and it is preferably about 1.0 units lower than the acid sickle value. The final acid value is too insoluble for the polymer and is therefore insoluble • and therefore gives physical stability to the particles when suspended in water. Other additives that can be added to the suspension; for example, compounds that reduce the tendency of the polymer to crosslink and produce a precipitation reaction when divalent cations appear, such as ethylenediaminetetracarboxylic acid. The dispersion solution of the non-light-emitting photoblocker printed in the polymer solution by the Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs is prepared by adding water to the polymer solution, and optionally, the dispersant is essentially acidic. . Diffusion agents are widely known in the art. The light blocker is added to the mixture later, preferably using a high tension mixer plus M. Finally, the mixture was sprayed dry, causing evaporation of large amounts of water and volatile radicals. In the traditional way, the particle size in the range of 1 to 50 mm can be obtained by adding K in the traditional way, and the diameter of the better medium (average volume) is about 10 to 40 microns. . It is better that the particle size distribution plaque is very narrow. Makes at least 60% of the particles and more preferably -17- the size of this paper applies the Chinese National Standard (CNS) M specifications (2IO × thin) B7 V. Description of the invention (丨 0 0% here is average or close to this average diameter. It should be emphasized that the composition of the present invention may include other traditional agents and additives. These elixirs include, but are not limited to, surfactants; Oxygen m stabilizers; such as propionic acid, fillers; for example, decellularized soybean meal, with fluidity and anti-adhesive agents; for example, artificially synthesized precipitated silicon, diffusion_; for example, naphthalenesulfonated naphthalenesulfonic acid And the like. The surfactant can be changed in a wide range of paintings. For the sake of convenience, some drugs can contain from about 0.1% to about 50% by weight of the composition, more preferably It is about 0.5% to about 40%, and most preferably 1% to about 20%. The composition of the present invention can be further formulated, and at the same time, those skilled in the art know that many biocides that make PK Product methods. These methods are described in technology and In the patent documents, M and M include fluids, concentrates, and similar substances that form granules, moist powders, M water as a matrix, and grease as a matrix. The following examples are provided for illustration, and are not intended Any way to limit the present invention. A. Unbleachable wet powder Unmixed mode: 63% active killing ingredient, 10% flow_ (precipitated silicon), 4% interfacial activated cocoon (naphthylsulfonate), 13% Diffusion agent (alkyl aromatic polyacetic acid), 7% (US activated clay), and 3% ethylenediaminetetracarboxylic acid (£ 014). Β. Granules: 6 3% active killing ingredient, 20 % Flowable (precipitated silicon), 3% interfacial activation fee | (Mortarized alkylbenzenesulfonate), 5% diluent (mixture of anionic interfacial activator and sodium sulfonate), and 9 % Carrier (»soil). -1 8-This paper size applies to Chinese National Standards (CNS) Λ4 is present (210X297 public housing-) Please read on the back page Printed by the Ministry of Economic Affairs Central Standardization Bureau Staff Consumer Cooperatives B7 Ministry of Economic Affairs Printed by the Consumer Affairs Cooperative of the Central Government Bureau V. Invention Description 丨 ⑴) The invented product of the invention contains active insecticidal ingredients and any further development can be applied to the method for controlling Kunnong pests in the field of controlling plaques. This includes applying a composition for killing effective directors in a region 9 of which The aforementioned areas include, but are not limited to, crops and habitats. The 100 镙 region is Lepidoptera > Diptera and m-finned Kun & Potential Buildings 9 including but not limited to the following: ♦ Goods such as wheat, corn, rice and rarely cotton i Leguminous plants t Oil-rich plants such as Xiangyue sunflower 1 m Vegetable function »Deciduous and pine tree plants Pupae and vines and freshwater plants. The effective amount is the active ingredient that causes the actual death rate of the quince. The amount of m 〇 This mix can be destructive »and can be used in advance-step diluted with water 0 as in each example When the application time is the same as the dilution in the method, the concentration will depend on the nature of the pathogen that is expected to be mined and the type of amaranth to be treated and will be very obvious to those who are exaggerated in this technique. 0 The following examples are more complete Describing specific specific examples of the present invention 0 As is well known to those skilled in the art, these examples are illustrative and are not meant to be limited to this. 0 In addition, at least 10 A in each of 1 A to D The finished product is prepared by using the described composition or the minor change of phase M plus M to obtain 0. Example 1 · Bovine killing net product A carbon black and plastic polypropylene. Heat 89 grams of water to Celsius 70 ° 9 At the same time, add 10 grams of carboxy polyacrylic acid (Carboset 5 2 5, BF Good ΓΙ ch-19-Please first Read the note on the back side This paper size applies to the Chinese National Standard (CNS) A4 specification (210 < 2W mm) Fen A7 B7 V. Description of the invention (丨 2)). 1 gram of a 30% aqueous ammonium hydroxide solution was added to a 5 equivalent volume and size aqueous solution over a 4 hour period. The solution (I) was left to stir overnight. Please read the note-item-item before filling in this page. Add 10 grams of carbon black (Printex P, Degussa) to 90% of water and mix with a high tension mixer for 3 hours. The carbon black diffusion solution (H) has an acid value of M25% aqueous solution sulfuric acid adjusted to 2.0. Add 16 grams of diffusion solution (H) to 4 g of solution (I) and mix with a high-tension mixer for at least 3 G minutes to form an acid «I value between about 5.9 to 6.4 in polymer solution (JI ) In carbon black. 8 grams of a powder preparation of S. thuringiensis Kotaski mutant strain SA 12 spores and crystals, containing CrylA (c) and CrylA (b) conjugated with 72 grams of water, and stirred for 2 hours. The pH value of this suspension was adjusted to 4.2 with a 25% aqueous solution of lyophilic acid and later adjusted to 5.0 with 30% aqueous ammonium hydroxide. This acid «value adjustment method is repeated twice or more. The acid Λ of the thuringiensis suspension should be 30% aqueous solution of argon ammonium oxide to be higher than the preparation solution (I) 0.1 units. It is produced by the shellfish consumer cooperation of the Central Bureau of Standards of the Ministry of Economic Affairs. Mixer for at least 45 minutes. The final mixture Buchi 190 Mini Spray Dyer reduces the atmospheric pressure by approximately 50 bar at a population air temperature of approximately 180 degrees Celsius • The radon speed of approximately 10 liters per minute, M4 The atmospheric pressure of Ba crushes into atoms and dries. • At the same time, the nozzle is adjusted to obtain a narrow size distribution with an average diameter of about 10 to 20 microns. This sample has half a period of exposure to sunlight at about 20 watts an hour / cm 2 (M360 麈 m wavelength_hang). Compared with a sample that does not contain black and its chemical component is about 10 microwatts an hour / About square centimeters. The method for measuring the half-life period has been published. 20 paper sizes are in accordance with the Chinese National Standard (CNS) Λ4 specification (210 X 2 mm). Printed on the A7 B7 by the Shellfish Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs. 5. Description of the invention (丨 /) It is described in the book Photo-stabi 1 ity Preparation of B. thuringiensis and Use of Light-absorbing Protectoits, Eighth I UP AC International Congress on Pesticide Cheaistry, July 1994, Washington DC) and has been included in the reference. B. The argon servants are good at a certain amount of propylene chloride: A rock killing solution (I) was prepared as described in Example 1. 4_2 grams of a powdered dish of Sulzer Kotaski spores SA 12 spores and crystal mash were mixed with 79.2 grams of water and allowed to stir for 2 hours. The acid sickle value of this suspension was adjusted to 4.2 with M25% aqueous solution, and later adjusted to 6.2 with 30% aqueous ammonium hydroxide network. Before the M30% aqueous solution "Ammonium Oxide adjusts the acid Mt value to 6.5 • This acid sickle value adjustment method is repeated 2 times on K. Add 4.6 grams of titanium dioxide (T r ο η ο X, li er r-McGee) to the thurium suspension, and mix with a K high-tension mixer to obtain a mineral solution (IV). After 0.4 g of ethylenediaminetetraacetic acid was mixed with 3.6 g of water, it was added to fluorene (IV). Immediately afterwards • Add 8 grams of polymer solution (I) and mix with a high tension mixer for at least 45 minutes. The final mixture was spray-dried according to the method described in Example 1A. The transcript is at about 20 watts an hour per square centimeter of daylight, compared with 10 watts an hour per square centimeter when compared with the same transcript that does not contain titanium dioxide. -2 The size of this paper is applicable to Zhongwei National Standard (CNS) M specifications (210 X 297 mm>)-(Please read the precautions on the back before filling this page): Packing ----

In-II— nn I--n-1—. 37 V. Description of the invention (') c · Carbon black and cage 7. Shop / _ type butene diether drunk co-party: Please read the notes on the back first. 10 grams of a styrene / cis-butanedioic anhydride copolymer (Scripset 5 2 0, Monsanto) was added to 83.2 grams of lightly agitated water. 6.8 grams of a 30% aqueous solution of ammonium hydroxide was added to the 10 equivalent space and size aqueous solution over 5 hours. This solution (V) was stirred overnight. 10 grams of carbon black was added to 90 grams of water and 0.2 grams of 98% sulfuric acid, and a high tension mixer was mixed for 3 hours to form a failure rate (VI). Add 16 liters of slush (VI) to 4 grams of solution (V), and mix with a high-tension mixer for at least 3Q minutes to obtain a carbon black in the polymer solution with a pH of about 5.9 to 6.4 (VI) Diffusion solution. 8 grams of a powder preparation of the Sulzer Kotaski spore SA 12 spores and crystal mash was mixed with 72 grams of water and stirred for 2 hours. The pH value of this suspension was adjusted to 4.2 by the acidic and salty M2 5 炻 aqueous solution, and later adjusted to 6.3 by the M 30% aqueous ammonium hydroxide solution. The acid value of the sulind solution was adjusted with a 30% aqueous ammonium hydroxide to the acid value of the preparation (VI), and the two were combined in a high-tension mixer for at least 45 minutes. Consumption cooperation by employees of the Central Bureau of Standards of the Ministry of Economic Affairs. The final mixture is a pulverized dry smelting device. The control process has a narrow size distribution with an average diameter of about 10 to 20 microns. The kneading paper has half a period under about 20 microwatts of one hour per square centimeter of sunlight, compared with 10 microwatts of one hour per square centimeter when compared with samples that do not contain carbon black but are otherwise the same. D. Congli "Carboxy" "Rui": The polymer solution (I) is prepared by adding 4 grams of carboxyl polypropylene-22-This paper size is applicable to Chinese national standards (CNS > A4 said grid (210X297 mm) ) Printed Λ7 B7 by the Consumer Cooperatives of the Central Government Bureau of the Ministry of Economic Affairs V. Description of the invention (y /) Acid (081 > 1) 〇861 525, 8.17.6〇〇 (11 ^ (; 11) in 22.7 grams of water, Raise the Zhan degree to about 7 Q degrees Celsius, stir gently, and add concentrated sodium oxide during about 1 to 8 hours to make the final acid value between about 7 and 8. This mixture is stirred later Hours or overnight M to ensure the complete dissolution of the polymer. Carbon black (Printex g, Degussa) in the polymer solution expansion solution (I) is prepared by adding 197.3 g of water to the polymer solution (I) And add Q. 6 6 filled lignosulfonate or similar diffusion liquid (for example (T a ο 1. SN 〇f R 〇h & Haas, P hi 1 ade 1 phia, PA 〇r Polyfon H 〇Westvace , Charleston Heigfits, SC), then add 33 filled carbon black and mix it with a high tension mixer. Preparation of ammonium sulphate (I) solution It is made by dissolving 4.2 grams of mash in 30 grams of water. 63 grams of the powdered preparation of spores and crystalline spores of S. sulitensis Kotaski spore SA 12 are mixed with 500 grams of water and mixed. Hold the record for 2 hours. This Sulina bacteria "Float (IV) is mixed with the ammonium sulfate solution in (I) * and the required concentrated hydrogenated hydrogen or sparse acid is used to adjust the acid susceptibility value to 6.3." The pre-slurry slurry was prepared by mixing the suspension of S. thuringiensis in (IV) with the carbon black diffusion solution of the polymer solution in (I). The pre-slurry slurry was sprayed as described in Example 1A above. No. This sample has half a period under sunlight of about 100 microwatts an hour per square centimeter. Compared to a sample that does not contain carbon black but has the same chemical content, it is 10 »watt an hour per square centimeter. E · Disc Considering the 茏 Zene / Phenanthenate Copolymer: The preparation is made from alfalfa silver worm polygonal virus I (NPV) as a 5.4% total weight aqueous suspension. A 100% Gram of carbon black (-23- This paper is sized to China State Standard (C > iS) Α4 size 丨 210 X 297 feet)--\\ · Γν (Please read the back first Please fill in this page again)-Packing. Order printed by the Consumers Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs, printed A7 B7 _V. Description of Invention (/ ^) Norit A) Expanded in 3 9 5 grams of water with 5 grams of diffusing agent (Morwet, Witco Corp) grinds into a rough surface all night. This 66.1 g expansion solution was later mixed with 66.1 g of the nuclear polyhedrosis virus suspension, and the acid M1 value was adjusted to 8.6 to generate a second diffusion solution. A styrene / co-butyric acid copolymer solution was prepared as described in Example 1C, except for 200 g of water. This polymer solution was mixed with a second diffusion solution * and the final mixture was dried as described in Example U. Example 2: The preparation of the sample of the grade 蛊 裨 is prepared as described in Example 1C above. In addition to the fan surface of different average glume size, it is from about 4 to 45 under different circumstances. Microns. This reduced atmospheric pressure is changed from about 5 2 to about 4 3 «bar, the sprinkler speed is changed from about 10 to about 7 l / min, and the pores are adjusted to obtain the desired narrow size distribution range. This sample M 0.2 炻 surfactant (Sylard 309, Dow Corning) was suspended in water and sprinkled on the broad-leaved cabbage at 6 to 10 flocks. After some storage has been suspected, the leaves are collected and infected with the most recent first stage Trichoplusia ni. After 4 hours of incubation, the lethal rate was measured and the probit calculation method described in Probit analysis (Finney, DJ, Probit Analysis, Cambridge University Press (1964)) was used to calculate the 50% lethal dose (see drawing 1) . A 50% lethal dose is defined as the concentration that causes 50% death in the population. This indicates that compared with the control group, the activity of Pujia is "Achieved by the present invention, excluding polymer M to prevent other spores-24. (Read the note on the back before filling in this page) • Package. Order " This paper is compliant with the China National Standard (CNS) Λ4 specification (2 丨 〇: < 297 mm.) A 7 B7 V. Description of the invention (> On the leaf surface, and thus exhibited a secondary lethal inhibitory feeding agent M. Example 3: The original ridge and sample of Yi aa 抻 ">: A sample was prepared as described in the above example ία, but modified as follows : Mix 4 grams of solution (I) with 48 grams of diluent (I), while mixing 4.8 grams of selenium powder with 43.2 grams of water. Suspend the final dry powder (boron) in suspension In water, and sulphur sprayed on the core leaves at a rate equivalent to 0.33 lbs / mu of S. coli as described in Example 2. A sample of control group K containing no carbon black or polymer was equivalent. At a speed of 0.8 5 lbs per tadpole, the selenium was also stabbed on the leaves of the core. This plant was exposed to sunlight and the leaves were harvested Set and infect with T. ni larvae plus M. Lethality (M untreated plants plus M correction) as a function of exposure_sunlight harvest is shown in Table 1. Table 1

Days Exposure to Sunlighe Mortality⑴ Please read first- | -read the back I notes

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Printed by the Central Standards Bureau of the Ministry of Economic Affairs

Encapsulated Un-encapsulated 0 100 100 2 97 78 3 100 48 4 93 37 5 100 37 6 83 10 7 78 0 -25- This paper size applies to China National Standard (CNS) Λ · 4 size (2 丨 OX 297) 〉 B7 V. Explanation of the invention (Chong) 8 80 Printing Example 4 by the Shellfish Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs: The mixture (K) of Tetradendron martensii and carbon black was mixed at a ratio of 1: 1 at 10 % Of the total solids concentration was sprayed onto the glass plate, and the half-life in daylight was about 26 watts an hour per square centimeter at a rate of 90 gallons per second, and when the concentration was reduced to 1% male When solid, the half-time period drops to about 15 microwatts per hour / cm 2. When the solid concentration on the glass is low, there is not enough carbon black close to the S. coli preparation M to provide effective protection K against sunlight. The rest of the The optical suppression of radon is not thick enough to absorb sufficient incident radiation at a sufficient ratio. In typical agricultural applications • When the concentration of radon is not exceeded! Ftl% In order to have any practical effect, the pesticide must be effective -Copy this method later in Example 1C plus K And the Ml% total solids concentration is sprayed on the glass slide, and M is equivalent to a spraying speed of 30 plus eaves / 玖. The half-time period under daylight is about 130 microwatts per hour per square centimeter. This result illustrates The utility of the polymer matrix lies in the closest contact between the spores and crystals of the male thuringia preparation and the particles of the inorganic light blocker, and shows how the adult product can provide effective protection against light exposure. Not activated. -2 6-This paper size is applicable to China National Standard (CNS) Α4 (210X297mm) Please read the cautions below

I Fen

Claims (1)

Qiqiu 229 Bulletin Patent application scope Printed by the Central Laboratories of the Ministry of Economic Affairs and Consumer Cooperatives 1 * An effective insecticidal composition of a biological insecticide, including: (a) 3 (U to 80% by weight of Bacillus) And an active insecticidal ingredient selected from the group of insecticidal active proteins of the genus Bacillus; (b) a polymer of 1X to 15¾ weight percent, which is soluble under neutral to experimental conditions, and It is insoluble in the case of weak acid in aqueous medium, and the polymer will form a matrix; and the polymer is a carboxy polyacrylic acid or a styrene / cis butadiene anhydride copolymer; (e) 5X to 60% by weight One percentage is selected from the inorganic light blocking agents of titanium dioxide, carbon black, perylene pigment and rhodopsin, wherein the light blocking agent diffuses with the active ingredients in the matrix formed by (b) the polymer, wherein the light blocking agent protects the activity The ingredients are protected from being inactivated by ultraviolet light or sunlight; and the composition is in the form of spray-dried granules. 2 · The composition in item 1 of the patent application, where the active ingredient is Suli Crystalline protein or spores or relative conjugates. 3 * The composition of any one of items 1 to 2 of the patent application, wherein the active ingredient is a variant of Bacillus thuringiensis var. kurstaki) strain 15 4. The composition according to any one of claims 1 to 2, wherein the active ingredient is derived from a strain of Bacillus thuringensis var. aizawai 13 5 * If the composition in the first item of the patent scope of Shenyin, where the activity is -1- This paper size is applicable to the middle and poor countries kneading rate (CNS) A4 specifications (210X297 mm) Please read the note before the I I Binding Qiqiu 229 Bulletin Patent application scope Printed by the Central Laboratories of the Ministry of Economic Affairs and Consumer Cooperatives 1 * An effective insecticidal composition of a biological insecticide, including: (a) 3 (U to 80% by weight of Bacillus) And an active insecticidal ingredient selected from the group of insecticidal active proteins of the genus Bacillus; (b) a polymer of 1X to 15¾ weight percent, which is soluble under neutral to experimental conditions, and It is insoluble in the case of weak acid in aqueous medium, and the polymer will form a matrix; and the polymer is a carboxy polyacrylic acid or a styrene / cis butadiene anhydride copolymer; (e) 5X to 60% by weight One percentage is selected from the inorganic light blocking agents of titanium dioxide, carbon black, perylene pigment and rhodopsin, wherein the light blocking agent diffuses with the active ingredients in the matrix formed by (b) the polymer, wherein the light blocking agent protects the activity The ingredients are protected from being inactivated by ultraviolet light or sunlight; and the composition is in the form of spray-dried granules. 2 · The composition in item 1 of the patent application, where the active ingredient is Suli Crystalline protein or spores or relative conjugates. 3 * The composition of any one of items 1 to 2 of the patent application, wherein the active ingredient is a variant of Bacillus thuringiensis var. kurstaki) strain 15 4. The composition according to any one of claims 1 to 2, wherein the active ingredient is derived from a strain of Bacillus thuringensis var. aizawai 13 5 * If the composition in the first item of the patent scope of Shenyin, where the activity is -1- This paper size is applicable to the middle and poor countries kneading rate (CNS) A4 specifications (210X297 mm) Please read the note before the I I Binding 385229 The patent application is derived from the recombinant strain of Bacillus. 6 * As the composition of the scope of patent application No. 1 in which the active ingredient is natural from Bacillus Derived from the occurring strain. 7 ♦ The composition of item 2 of the patent application scope, wherein the active ingredient is crystalline protein 霣 IC (CrylC), crystalline protein IA (a) (CryIA (a)) 'Crystalline protein IA (b) (CrylA (b)) * Crystal protein IA (C) UrylA (c)), crystalline protein IIA (CryIIA) and crystalline protein IE (CrylE), and a group consisting of a mixture or a phase contrasting group 8 can be obtained. Of the composition * wherein the socks-free light blocker is carbon black, titanium dioxide or a mixture of similar materials. 9 · The composition according to item 1 of the patent application park, wherein the composition is further formulated into granules, wettable powder, K Water is a matrix flowable formulation or M oil is a matrix flowable formulation. 10 ♦ —A method for controlling insect pests in the area to be controlled. This method includes applying a group such as any one of items 1 to 9 of the scope of patent application. (Please read the Note f on the back • This page-book-The Central Bureau of Standards of the Ministry of Economic Affairs * C Industrial Consumer Cooperatives printed the 9-item item in the Pest Kuno 〇 〇1 in the domain enclosure should be conducive to special Please apply to be like a worm, kill 1 of 1 to become a giant 0 0 scales are insect pests, insect pests, insects, insects, and other items in the * method ο No. 8 8 Fan Li specially requested Shen Ru 2 orders wing sheaths are insects including 3 The composition of the French object is 0. Insect pests and insecticides} Killing of te physin 0P prepared by ο -1 ^ ca This paper size is in accordance with Chinese National Standard (CNS) A4 size (210X297 mm) 385229 A8 B8 C8 _____ D8 VI. Scope of patent application (a) A polymer is formed, in which the polymer is soluble in an aqueous medium state, soluble in neutral to test conditions, and insoluble in weakly acidic conditions ; (B) a diffusing fluid which does not contain a tritium light blocker; (c) Obtaining a suspension culture liquid selected from the bacillus pupa and the active insecticidal component selected from the insecticidal active protein population of the bacillus pupa; U) preparing a volatile hydrazone-based solution, and combining the foregoing solution with step (c) (E) Mixing the light-blocking agent's diffusing solution with the polymer solution to obtain a second diffusivity, and later mixing the second diffusing solution with a suspension of the active ingredient and a volatile amidine-based solution Mix; and (f) (e) the spray-dried mixture in which the active ingredient and light blocking agent are diffused into the matrix formed by the polymer. 1 4 ♦ The method according to item 13 of the patent application scope, wherein the bacterium is a thuringiensis that includes a pesticidal protein or a spore or a mixture of spores. Printed by the Central Labor Bureau of the Ministry of Economic Affairs, Shellfish Consumer Cooperative, 1 5 ♦ As the method in the scope of application for patent No. 14 wherein the light blocker is carbon black or titanium dioxide or related mixtures, and contains about 5 to 5 60%. 16. The method of claim 15 in which the polymer is a copolymer of carboxy polyacrylic acid or styrene maleic anhydride, and contains 1 to 15% of the composition. 17 · —Method for preparing effective insecticidal composition of biological insecticides -3- This paper size is printed with Chinese National Standard (CNS) A4 ^ (210X297 mm) Printed by the Central Standards Bureau of the Ministry of Economy Policy A8385229 cl 6. The scope of patent application, which includes: (a) Obtaining a suspension culture of S. thuringiensis which contains insecticidal peptone or spores or related mixtures as an active ingredient, and a socks-free light blocker with this The suspensions are mixed; (b) forming a polymer solution in which the polymer is soluble under neutral to alkaline conditions in an aqueous medium mode and insoluble under weak acid conditions, where the polymer is A copolymer of carboxy polyacrylic acid or styrene maleic anhydride and containing 3 to 10% of the composition; (c) mixing the mixture of (a) with the solution of (b) to obtain a suspension; and (d) The suspension is spray-dried, in which the active ingredient and the light-blocking agent are dispersed in a matrix formed by the polymer. 1 " (Please read the note on the back of this page first page) Order--4- This paper size uses the Chinese National Standard (CNS) A4 specification (210X297 mm)