TW375656B - High-proliferative mutants of saccharomyces cerevisiae and induction method thereof, expression host derived therefrom and its use in over-expression of foreign proteins - Google Patents
High-proliferative mutants of saccharomyces cerevisiae and induction method thereof, expression host derived therefrom and its use in over-expression of foreign proteinsInfo
- Publication number
- TW375656B TW375656B TW085102878A TW85102878A TW375656B TW 375656 B TW375656 B TW 375656B TW 085102878 A TW085102878 A TW 085102878A TW 85102878 A TW85102878 A TW 85102878A TW 375656 B TW375656 B TW 375656B
- Authority
- TW
- Taiwan
- Prior art keywords
- expression
- novel
- mutants
- saccharomyces cerevisiae
- growth
- Prior art date
Links
Abstract
This invention provides a novel induction method to solve the problem of overproduction of HbsAg employing genetic engineering on saccharomyces cerevisiae, normally resulting in the death of the host cell. In the invention, these dying cultures are grown for about 25 days. It is found that some cells are recovered and new mutants of transformants are obtained. The behavior of each mutant is different from the normal strains. They are resistant to the cytotoxicity of HbsAg expressed in yeast secretion pathway and have higher growth ability and higher viability than normal yeast transformants. The mutated transformant Pyas/12S(KC) is a representative. The invention also provides the plasmid-cured strain KC-1. It maintains the aforementioned characteristics and can be used as a host strain of the yeast genetic engineering system for expression of heterologous proteins. The cell size of KC-1 is about 3 times bigger; the growth is about 1.5 times faster and the cell number is about 5-10 times higher than the parent strain (20B12). KC-1 changes the optimum growth temperature from 28 degree C to 37 degree C can grow in low nitrogen requirement and get into filamentous growth. Therefore these mutants can be used in the basic study of molecular and cell biology of saccharomyces cerevisiae, and directly applied to baking, alcohol production and wine brewing. In addition, a novel powerful host system is provided to express higher HbsAg or alpha-amylase genes is constructed in the vector system of S. cerevisiae, and transferred into the novel mutant. The yield obtained by the novel transformant is higher than that by the control strains.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TW085102878A TW375656B (en) | 1996-03-09 | 1996-03-09 | High-proliferative mutants of saccharomyces cerevisiae and induction method thereof, expression host derived therefrom and its use in over-expression of foreign proteins |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
TW085102878A TW375656B (en) | 1996-03-09 | 1996-03-09 | High-proliferative mutants of saccharomyces cerevisiae and induction method thereof, expression host derived therefrom and its use in over-expression of foreign proteins |
Publications (1)
Publication Number | Publication Date |
---|---|
TW375656B true TW375656B (en) | 1999-12-01 |
Family
ID=57941905
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
TW085102878A TW375656B (en) | 1996-03-09 | 1996-03-09 | High-proliferative mutants of saccharomyces cerevisiae and induction method thereof, expression host derived therefrom and its use in over-expression of foreign proteins |
Country Status (1)
Country | Link |
---|---|
TW (1) | TW375656B (en) |
-
1996
- 1996-03-09 TW TW085102878A patent/TW375656B/en not_active IP Right Cessation
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Longo et al. | Chromosomal rearrangements during vegetative growth of a wild strain of Saccharomyces cerevisiae | |
EP0438200A1 (en) | Method for the expression of heterologous genes in the yeast Pichia pastoris, expression vectors and transformed microorganisms | |
US20160355831A1 (en) | Mortierella alpine uracil auxotroph with ura5 gene knocked out through homologous recombination, and construction method thereof | |
Bao et al. | Secretion of human proteins from yeast: stimulation by duplication of polyubiquitin and protein disulfide isomerase genes in Kluyveromyces lactis | |
AU4195099A (en) | Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells | |
CN1578839B (en) | Modulating urea degradation in wine yeast | |
JP4526638B2 (en) | High protein expression system | |
TW375656B (en) | High-proliferative mutants of saccharomyces cerevisiae and induction method thereof, expression host derived therefrom and its use in over-expression of foreign proteins | |
Kannan et al. | Influence of intra-and extracellular sucrases of Zymomonas mobilison the ethanol production and by-product formation | |
KR940004051A (en) | Yeast Host Strains Deficient in N-glycosylation | |
Nancib et al. | Effect of growth rate on stability and gene expression of a recombinant plasmid during continuous culture of Escherichia coli in a non-selective medium | |
Stewart et al. | Current developments in the genetic manipulation of brewing yeast strains—A review | |
CN102120966A (en) | Construction and application of URA3 defective P. pastoris X-33 strain | |
KR101249778B1 (en) | Yeast Variants Having Resistance to Low Temperatures | |
KR101264294B1 (en) | Yeast Variants Having Resistance to Low Temperatures | |
US6284490B1 (en) | Asporogenous strain of bacillus subtilis and its use as a host for the preparation of heterologous products | |
KR100545563B1 (en) | A novel saccaromyces cerevisiae strain with enhanced recombinant protein production capability | |
Titorenko et al. | Peroxisome biogenesis in Hansenula polymorpha: different mutations in genes, essential for peroxisome biogenesis, cause different peroxisomal mutant phenotypes | |
CN102268431B (en) | Orotidine-5'-phosphate decarboxylase promoter (pRtura3), application thereof, construct thereof and vector thereof | |
Salek et al. | Stably inherited killer activity in industrial yeast strains obtained by electrotransformation | |
US7550281B2 (en) | Method for production of alpha-amylase in recombinant Bacillus | |
Johnston | Genetic analysis of spontaneous half-sectored colonies of Saccharomyces cerevisiae | |
Chaves et al. | Studies on growth kinetics and plasmid stability of a recombinant Escherichia coli expressing a Schistosoma mansoni antigen | |
Dhamija et al. | Thermotolerance and ethanol production are at variance in mutants of Schizosaccharomyces pombe | |
WO2000042203A3 (en) | Methods for producing polypeptides in cyclohexadepsipeptide-deficient cells |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
MK4A | Expiration of patent term of an invention patent |