TW375656B - High-proliferative mutants of saccharomyces cerevisiae and induction method thereof, expression host derived therefrom and its use in over-expression of foreign proteins - Google Patents

High-proliferative mutants of saccharomyces cerevisiae and induction method thereof, expression host derived therefrom and its use in over-expression of foreign proteins

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Publication number
TW375656B
TW375656B TW085102878A TW85102878A TW375656B TW 375656 B TW375656 B TW 375656B TW 085102878 A TW085102878 A TW 085102878A TW 85102878 A TW85102878 A TW 85102878A TW 375656 B TW375656 B TW 375656B
Authority
TW
Taiwan
Prior art keywords
expression
novel
mutants
saccharomyces cerevisiae
growth
Prior art date
Application number
TW085102878A
Other languages
Chinese (zh)
Inventor
Tsong-Teh Kuo
Tzu-Chih Chen
Original Assignee
Nat Science Council
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Publication date
Application filed by Nat Science Council filed Critical Nat Science Council
Priority to TW085102878A priority Critical patent/TW375656B/en
Application granted granted Critical
Publication of TW375656B publication Critical patent/TW375656B/en

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Abstract

This invention provides a novel induction method to solve the problem of overproduction of HbsAg employing genetic engineering on saccharomyces cerevisiae, normally resulting in the death of the host cell. In the invention, these dying cultures are grown for about 25 days. It is found that some cells are recovered and new mutants of transformants are obtained. The behavior of each mutant is different from the normal strains. They are resistant to the cytotoxicity of HbsAg expressed in yeast secretion pathway and have higher growth ability and higher viability than normal yeast transformants. The mutated transformant Pyas/12S(KC) is a representative. The invention also provides the plasmid-cured strain KC-1. It maintains the aforementioned characteristics and can be used as a host strain of the yeast genetic engineering system for expression of heterologous proteins. The cell size of KC-1 is about 3 times bigger; the growth is about 1.5 times faster and the cell number is about 5-10 times higher than the parent strain (20B12). KC-1 changes the optimum growth temperature from 28 degree C to 37 degree C can grow in low nitrogen requirement and get into filamentous growth. Therefore these mutants can be used in the basic study of molecular and cell biology of saccharomyces cerevisiae, and directly applied to baking, alcohol production and wine brewing. In addition, a novel powerful host system is provided to express higher HbsAg or alpha-amylase genes is constructed in the vector system of S. cerevisiae, and transferred into the novel mutant. The yield obtained by the novel transformant is higher than that by the control strains.
TW085102878A 1996-03-09 1996-03-09 High-proliferative mutants of saccharomyces cerevisiae and induction method thereof, expression host derived therefrom and its use in over-expression of foreign proteins TW375656B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
TW085102878A TW375656B (en) 1996-03-09 1996-03-09 High-proliferative mutants of saccharomyces cerevisiae and induction method thereof, expression host derived therefrom and its use in over-expression of foreign proteins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
TW085102878A TW375656B (en) 1996-03-09 1996-03-09 High-proliferative mutants of saccharomyces cerevisiae and induction method thereof, expression host derived therefrom and its use in over-expression of foreign proteins

Publications (1)

Publication Number Publication Date
TW375656B true TW375656B (en) 1999-12-01

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Family Applications (1)

Application Number Title Priority Date Filing Date
TW085102878A TW375656B (en) 1996-03-09 1996-03-09 High-proliferative mutants of saccharomyces cerevisiae and induction method thereof, expression host derived therefrom and its use in over-expression of foreign proteins

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TW (1) TW375656B (en)

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