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Priority claimed from US08/785,324external-prioritypatent/US5795469A/en
Application filed by Cohesive Tech IncfiledCriticalCohesive Tech Inc
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Publication of TW349029BpublicationCriticalpatent/TW349029B/en
A high performance liquid chromatography method using a chromatography column containing chromatographically reactive surfaces, the method comprising the following steps: flowing a discrete volume of liquid mixture containing at least one solute that is reactive with the surfaces so as to load the column, so that the surfaces elute the solute adhered thereto by flowing the eluant through the column. The improvements of the method comprising the following steps: injecting at least one discrete plug of the eluant fluid into the column adjacent the input of the column so as to maintain minimized spatial step separation between the plug and the discrete volume of liquid mixture as the plug and volume traverse the column.
TW086108288A1996-09-301997-06-16High performance liquid chromatography method and apparatus
TW349029B
(en)