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A self-contained on-line sampling apparatus, which comprises: (a) a separation valve; (b) a throttle valve; (c) a sampling chamber used for a sampling valve or a photo-sensitive analysis instrument; and (d) a variable volume device comprising a chamber and a reciprocating piston; in which the separation valve, the throttle valve, the sampling valve and the variable volume device are connected in series and undergo fluid exchange through a fluid exchange device, and the self-contained on-line sampling apparatus further comprises: (e) a pressure sensor undergoing fluid exchange with the components of (a) to (d); and (f) a pressure controller undergoing fluid exchange with the pressure sensor and the variable volume device.