TW202404471A - Plant growth regulator containing viable bacteria or culture of bacterial strain of genus bacillus as active component, and method of use thereof - Google Patents

Abstract

An object of the present invention is to provide a naturally derived, safe-to-use microbial material that can contribute to improving the agricultural output by regulating crop growth, and imparting resistance against environmental stresses to increase the yield while controlling plant disease at the same time. Among the solutions provided by the invention are, for example, a plant growth regulator and/or a plant disease control agent that regulate crop growth, and impart resistance against environmental stresses to increase the yield by containing viable bacteria of specific bacterial strains of genus Bacillus, or a culture containing such viable bacteria, as an active component.

Description

Plant growth regulators containing live bacteria or strain cultures of the genus Bacillus as active ingredients and methods of use thereof

The present invention relates to plant growth regulators containing live bacteria or strain cultures of the genus Bacillus as active ingredients, and their use.

Increasing crop yields has become increasingly important given the growing world population and global desertification. As climate change due to global warming is expected to increase, it is also important to make crops resistant to environmental changes. Breeding, fertilization, and cultivation techniques have been improved over the years to achieve high yields. However, there is also a growing need for newer and more versatile tools. In the field of agrochemicals, the development of plant growth regulators has been ongoing; however, in the past few years, increasing concerns about environmental issues have caused a high demand for materials of natural origin.

Among such natural materials, there are Mamerich ^® for soybeans and Yume-bio ^® for rice cultivation, both of which were recently developed using microorganisms. However, this only applies to limited types of crops.

Some strains of the genus Bacillus possess disease control activity and have been widely used for disease control.

Some such Bacillus strains used for disease control have been found to promote the germination of plant seeds (Patent Document 1). However, what is less well known is that Bacillus species can directly increase crop yields.

Based on this technical background, the inventors of the present invention conducted various studies to find ways to increase crop yields, and by focusing on plant growth regulation effects and plant disease control effects, they fully realized the need to develop products that can provide at least one of these effects. A medicine for one. In consideration of SDGs, such as safety assurance and prevention of environmental pollution, the inventors are also keenly aware of the need to develop pharmaceuticals of natural origin. The present invention is motivated by these needs, and one of the objects of the present invention is the development of such materials.

Crop disease damage also remains a challenging problem, and various approaches have been taken to address this problem, including tillage or physical control using rotational cropping or solar heat, chemical control using chemical pesticides, and the use of disease resistance Control of disease-resistant breeds. However, this cannot be said to be satisfactory. Control by chemical pesticides has become a serious global problem due to the emergence of drug-resistant bacteria that potentially weaken the effectiveness of chemical pesticides.

After extensive research in search of solutions to the aforementioned technical problems, the inventors have concluded that microorganisms are suitable as active components of novel plant growth regulators. Our extensive screening identified the Bacillus sp. D747 strain of the genus Bacillus, and this successfully led to the development of a novel plant growth containing live bacteria or cultures of this strain as an active ingredient Conditioner.

At the same time, severe environmental changes represented by global warming in recent years have accelerated desertification due to drought and frequently caused natural disasters, such as farmland flooding due to heavy rains and rising sea water levels. The impact of such environmental stress on crops is inevitable. For example, high temperatures and water can affect crops by causing stress. Measures must be taken to mitigate the effects of such pressures, such as in the former case by irrigation or afforestation of the desert, and in the latter case by drainage, such as surface drainage and subsurface drainage. Drainage (subsurface drainage). Other practices include crop breeding. However, these traditional techniques have disadvantages, specifically, agricultural engineering techniques are expensive and breeding requires a considerable amount of time to complete.

The inventors further studied Bacillus sp. D747 as an active component of the plant growth regulator according to the present invention. Quite unexpectedly, this research led to the unprecedented discovery that this strain confers resistance to environmental stress to crops with its new and highly efficient activities and properties.

The present invention was completed after further research based on this novel discovery, and provides a novel, useful, and highly safe resistance imparting agent (resistance imparting agent), especially the resistance imparting agent that confers resistance to environmental stress in crops. Reference list patent documents

PTL 1: JP2006-124280A non-patent literature

NPL 1: Green Report, No.621, p.21 NPL 2: The Journal of the Science of Soil and Manure, Japan, Vol.92, No.1, p.36 to 41

technical issues

The purpose of the present invention is to provide naturally derived, safe and available microbial materials that can increase yields by regulating crop growth and conferring resistance to environmental stress, and at the same time control plant diseases to help improve agricultural output.

Global warming is an important issue today, which has led to an increase in the occurrence of crop diseases and insect pests. This has created a need for the use of stronger control agents. On the other hand, crops need to be resistant to the intense heat stress caused by warming temperatures. Against this background, the inventor has established a new goal to increase crop yields by overcoming these problems, and using natural microorganisms as safe active ingredients to create a product that has plant growth regulation effects and plant disease control. New materials with advanced active effects. As discussed above, the present invention sets up the highly challenging goal of dealing with multiple situations simultaneously, and it can be said that the present invention is novel in terms of the object itself, or the goals it is intended to achieve. In other words, one aspect of the present invention plans to cope with the effects of global warming by providing "a powerful control effect on the increase in pests, etc." and "conferring resistance to high temperature stress to plants", and at the same time by providing Resistance to various environmental stresses to cope with the effects of warming temperatures, such as by "conferring resistance to aquatic stress to cope with increased flooding." problem solution

The inventors have conducted various studies to achieve the aforementioned objectives and have discovered that live Bacillus bacteria or cultures used for disease control have the ability to increase yields by regulating crop growth and imparting resistance to environmental stress. effect. Furthermore, the present inventors discovered ways to exploit this effect. The present invention was completed based on these findings.

Specifically, specific embodiments of the present invention are as follows. (1) A plant growth regulator, characterized by containing live bacteria or cultures of Bacillus subtilis or Bacillus amyloliquefaciens as an active component, and having a plant growth promoting effect or a growth inhibiting effect. (2) A plant growth regulator, characterized by comprising a bacterium or culture of one or more of the live Bacillus genus D747 strain, Bacillus subtilis HAI-0404 strain, Bacillus subtilis Y1336 strain, and Bacillus subtilis F727 strain as a Active ingredients, and have plant growth promoting effect or growth inhibiting effect. (3) A plant growth regulator characterized by containing a living bacterium or culture of the genus Bacillus D747 strain as an active component and having a plant growth promoting effect or a growth inhibiting effect. (4) The regulator according to any one of (1) to (3), wherein the regulator confers resistance to environmental stress to the plant in addition to a plant growth promoting effect or a growth inhibiting effect. (5) The regulator according to any one of (1) to (4), wherein the regulator has a plant disease control effect in addition to a plant growth promotion effect or a growth inhibition effect. (6) The regulator according to any one of (1) to (5), wherein the regulator regulates the growth of plants of the family Amaranthaceae (including plants of the family Chenopodiaceae). (7) The regulator according to any one of (1) to (5), wherein the regulator regulates the growth of Solanaceae plants. (8) The regulator according to any one of (1) to (5), wherein the regulator regulates the growth of Fabaceae plants. (9) The regulator according to any one of (1) to (5), wherein the regulator regulates the growth of Brassicaceae plants. (10) A method for regulating plant growth and/or conferring resistance to environmental stress and/or preventing plant diseases, characterized by comprising a plant growth regulator as any one of (1) to (9) One or more of them come into contact with the plant and/or soil (especially the rhizosphere). (11) The method according to (10), wherein the plant is a plant seed. (12) A seed treated as in (11). (13) The method according to (10), wherein the plant growth regulator is contacted with the soil through irrigation treatment or mixing into the soil treatment. (14) A pharmaceutical agent for imparting resistance to environmental stress to plants, characterized in that it contains as an active component a living bacterium or culture of the genus Bacillus D747 strain. (15) For the agent according to (14), the plant environmental pressure is high temperature pressure. (16) For the agent according to (14), the plant environmental pressure is aquatic pressure. (17) A method for imparting resistance to environmental stress to plants, characterized by the use of agents as in (14). Beneficial effects of the present invention

By simply using plant growth regulators containing live single or multiple naturally derived strains or cultures as active ingredients, the present invention can increase yields by regulating crop growth and conferring resistance to environmental stress while controlling plant diseases. . That is, the present invention can provide a material that contributes to crop production through the combined effects of two approaches: increasing profits through plant growth regulating effects and conferring resistance to environmental stress, and reducing profits through disease control. reduce.

Examples of environmental stress in the present invention include, but are not limited to, high temperature pressure, low temperature pressure, aquatic stress, drying stress, salt stress, ultraviolet pressure, and wind pressure. Notable examples are high temperature pressure and aquatic pressure.

Bacillus strain D747 represents an example of one of the active components of the present invention. The D747 strain was isolated from the air of Kikukawa City, Shizuoka Prefecture, and has been internationally deposited as Bacillus genus D747 (FERM BP-8234) at the National Institute of Advanced Industrial Science and Technology International Patent Organism Depository Center (The National Institute of Advanced Industrial Science and Technology) of Advanced Industrial Science and Technology International Patent Organism Depositary (now the National Institute of Technology and Evaluation, NITE Patent Organism Depositary): 2-5-8, Kazusa- Kamatari, Kisarazu-shi, Chiba, Japan, 292-0818; date of deposit: November 28, 2000). As described below, this strain is commercially available and readily available to anyone.

The bacterial properties of the Bacillus genus D747 strain are as follows. Bacterial properties were tested in accordance with Bergey's Manual of systematic Bacteriology, Volume 1 (1984).

(A) Morphology Morphology: rod-shaped Dimensions: 1.0 to 1.2 μm wide, 3 to 5 μm long Sportiness: + Attachment state of flagella: peritrichous flagella Endospores: + Spore position: center Spore Expansion:-

(B) Culture properties Colony color: white to light yellow Bouillon agar plate culture: forms white to cream-colored colonies with a wrinkled surface

(C) Physical properties Gram staining: + Nitrate reduction: + MR Test:- VP test: + Indole produces:- Starch hydrolysis: + Citric acid assimilation: + Inorganic nitrogen source: + Oxidase:- Catalase: + Growth pH 6.8, meat extract culture medium: + 5.7, meat extract culture medium: + growth temperature 30℃：+ 50℃：- Growth NaCl concentration 2%：+ 5%：+ 7%：+ Aerobic growth: + Anaerobic growth:- O-F test: 0 Yolk reaction:- Acid production from glucose: + Acid production from mannitol:- Acid production from L-arabinose:- Acid production from D-xylose:- Gas production from glucose:- β-Galactosidase:- NaCl and KCl requirements:-

Bacillus strain D747 is commercially available under the trade name ^Ecoshot® . In addition to the D747 strain, for example, the Bacillus subtilis HAI-0404 strain, the Bacillus subtilis Y1336 strain, and the Bacillus subtilis F727 strain can also be used in the present invention. Bacillus subtilis strain HAI-0404, Bacillus subtilis strain Y1336, and Bacillus subtilis strain F727 are commercially available under the tradenames ^Agrocare® wettable powder, ^Bachister® wettable powder, and ^Stargus®, respectively. In this specification, the term Bacillus may be spelled in different ways (eg Bashilus, Bachilus, Bachilas). However, these are spelling variations of the scientific name "(Bacillus)" in Japanese and all mean "Bacillus".

One embodiment of the invention is one that contains viable bacteria or cultures of the genus Bacillus D747 as the active ingredient. The plant growth regulator of the present invention can be used with the D747 strain alone, or can be used with bacteria or cultures of live D747 strain mutants (either alone or together with live bacteria or cultures of the D747 strain). Such mutants possess bacteriological properties of the D747 strain and exhibit plant growth regulatory effects. Mutants can be spontaneous mutant strains, mutant strains that have been mutated by ultraviolet light or chemical mutagens, or cell hybrid strains, and genetically recombinant strains. The D747 strain contained in the plant growth regulator of the present invention includes mutants of the D747 strain.

The culture used in the present invention is obtained from a bacterial culture of the genus Bacillus. Here, "culture" means a mixture of bacterial cells and culture medium. Bacillus can be cultivated according to the following methods commonly used for cultivating Bacillus, such as liquid culture (such as reciprocal shake culture, jar fermenter culture, and tank culture), and solid culture . Examples of culture media include general culture media, such as meat extract culture media, and media containing glucose, peptone, or yeast extract. In addition to liquid media, solid media may also be used, such as slant medium or plate medium supplemented with agar. The desired culture can be obtained by culturing and growing Bacillus.

As the carbon source of the culture medium, any carbon source that can be assimilated by the aforementioned bacterial strain can be used. Specific examples include various synthetic and natural carbon sources that can be utilized by strains of the genus Bacillus, such as glucose, arabinose, mannose, starch hydrolysate, and molass. Nitrogen sources in the culture medium can be selected from a variety of synthetic and natural materials that can be utilized by the bacterial strain, including, for example, organic nitrogen-containing products such as proteinaceous extracts, meat extracts, yeast extracts, and soybean meal. In addition, if necessary, trace amounts of nutrient sources can be added according to conventional methods of microbial culture. Examples of such nutrient sources include inorganic salts such as table salt and phosphate; metal salts such as calcium salts, magnesium salts, and iron salts; vitamins, amino acids, and nucleic acid-related substances. Various additives may also be added if necessary, such as, for example, defoaming agents.

Culture can be performed under aerobic conditions, such as by shake culture or aeration culture. The suitable culture temperature is 20 to 30°C, preferably 25 to 30°C. The suitable pH range is 5 to 8, preferably 6 to 7. The suitable cultivation period is 1 to 4 days, preferably 2 to 3 days.

After culturing in the above manner, the Bacillus strain of the invention can be used as an active component of a plant growth regulator in the form of a culture containing viable bacteria of this strain without isolating the strain from the culture. . Alternatively, viable bacteria can be isolated from the culture using conventional methods, such as, for example, by membrane separation or centrifugation, and the isolated bacteria, after washing if necessary, can be used alone or in processing (e.g., as a reagent with other components). mixture) is then used as the active ingredient. Bacterial cultures or isolated viable bacteria may also be in the form of a dry product obtained by using techniques such as freeze drying or spray drying, or by dilution of cultured bacteria or isolated bacteria in liquid or solid form. Use in dilute form as obtained. Cultured bacteria or isolated bacteria can also be used in the form of various preparations produced by mixing various additives using conventional methods for producing pesticide formulations. Examples of such formulations include granular formulations, emulsions, wettable powders, and flowable formulations.

The concentration of live bacteria contained in the plant growth regulator of the present invention is not particularly limited as long as the agent can produce the desired effect. However, because bacterial concentrations that are too low often fail to produce sufficient results, and bacterial concentrations that are too high usually waste bacteria, in the case of liquid preparations, for example, the bacterial concentration can be appropriately adjusted between 1×10 ⁵ and 1×10 ¹¹ cfu /ml, preferably in the range of 1×10 ⁶ to 1×10 ¹⁰ cfu/ml. As used herein, "cfu" means colony forming unit. When cultures are used, they can be appropriately designed based on viable bacterial concentrations in these ranges.

Examples of plants for which plant growth regulators of the present invention are expected to provide growth regulation, resistance to environmental stress, and disease control include cereals (e.g., rice, wheat, barley, corn, and buckwheat), potatoes (e.g., potato, sweet potato) , taro, and yam), peas (such as soybeans, kidney beans, azuki beans, and peas), vegetables (such as melon, tomato, eggplant, green pepper, cabbage, napa cabbage, radish, lettuce, Carrots, shallots, onions, strawberries, spinach, beets, chard, chard, and celery), fruits (such as apples, pears, cherries, peaches, grapes, oriental persimmons, citrus fruits, and kiwi fruits), industrial crops (such as cotton, rapeseed, common sunflowers, sugar beets, sugar cane, and tobacco), lawn grass (lawn grass), trees, and ornamental plants (such as roses, mums, tulips, gypsophila ( baby's breath), and lisianthus). However, the present invention is not limited to these embodiments.

Specific examples of plant pathogens that can be controlled by the plant growth regulator, and/or plant disease control agent, and/or environmental stress imparting agent of the present invention include Pseudoperonospora (such as Pseudoperonospora cubensis). )), Venturia (e.g. Venturia inaequalis), Erysiphe (e.g. Erysiphe graminis), Pyricularia (e.g. Pyricularia oryzae) Pyricularia oryzae), Botrytis (such as Botrytis cinerea), Rhizoctonia (such as Rhizoctonia solani), Cladosporium (such as Cladosporium solani) Cladosporium fulvum, Colletotrichum (such as Colletotrichum fragariae), Puccinia (such as Puccinia recondita), Septoria (e.g. Septoria nodorum), Sclerotinia (e.g. Sclerotinia sclerotiorum), Pythium (e.g. Pythium debaryanum Hesse), and Gaeumannomyces (eg Gaeumannomyces graminis). Examples of bacteria include Peudomonas syringae. However, the present invention is not limited to these examples.

The plant growth regulator, and/or environmental stress resistance-conferring agent, and/or plant disease control agent according to the present invention may be applied as such or after dilution with, for example, water. The use as a pesticide formulation is not particularly limited, and for example, plant growth regulators, environmental stress resistance imparting agents, or plant disease control agents can be sprayed onto crops or seeds, or crops or seeds can be soaked in plant growth regulators. agents, environmental stress resistance conferring agents, or plant disease control agents for direct application to crops or seeds. As another example, a plant growth regulator, an environmental stress resistance conferring agent, or a plant disease control agent may be sprayed onto the soil, or may be by irrigating the soil, or by application to water to be applied to crops and soil, or Fertilizer is applied. As yet another example, plant growth regulators, environmental stress resistance conferring agents, or plant disease control agents can be applied to agricultural implements. Preferably, the plant growth regulator, environmental stress resistance imparting agent, or plant disease control agent is sprayed directly to the crops. That is, by being present on the plant (for example, on the roots, stems, leaves, or seeds of the plant), or present in the soil used for cultivation, the plant growth regulator according to the present invention, and/or the environmental stress resistance The imparting agent and/or plant disease control agent regulates plant growth and imparts resistance to environmental stress to increase yield while suppressing plant diseases.

The dosage of the pesticide formulation of the present invention depends on factors such as the type of crop of interest, type of disease to be controlled, application method, disease occurrence pattern, degree of damage, environmental conditions, and formulation form. Therefore, no specific amount is set, and the pesticide formulation is preferably used in an appropriately adjusted amount. As an example, in the case of a liquid formula, the pesticide formula is used in an amount of 10 ml to 1 L per crop plant, preferably 50 ml to 1 L. The timing of application of a pesticide formulation also depends on factors such as the type of disease to be controlled, and the form of the pesticide formulation. Preferably, the pesticide formula is applied at an appropriate time before planting or within 2 weeks after planting. In the present invention, there is no concern about the emergence of resistant plant pathogens, and the microbial material of the present invention can be used for several days or can also be used for continuous cropping.

If necessary, the plant growth regulator, and/or environmental stress resistance imparting agent, and/or plant disease control agent according to the present invention can be used together with other fertilizers or agricultural chemicals, for example, such as fungicides, antiviral agents , insecticides, acaricides, nematicides, synergists, attractants, herbicides, and plant growth regulators. In this case, these may be applied after mixing with little effect on the active component strain, or may be applied at the same time or at different times.

The following compounds are non-limiting examples of known fungicides (bactericidal active ingredients) and disease control agents that can be used as mixtures or in combination.

Bactericidal active ingredients and disease control agents: Agrobacterim radiobacter, azaconazole, acibenzolar-S-methyl, azoxystrobin, anilazine, amethos( amisulbrom), aminopyrifen, ametoctradin, aldimorph, isotianil, isopyrazam, isofetamid, Isoflucypram, isoprothiolane, ipconazole, ipflufenoquin, ipfentrifluconazole, iprodione, iprovalicarb ), iprobenfos, imazalil, iminoctadine-albesilate, iminoctadine-triacetate, imibenconazole, inpyrfluxam, imprimatin A, imprimatin B, edifenphos, etaconazole, ethaboxam, erythromycin Ethirimol, ethoxyquin, etridiazole, enestroburin, enoxastrobin, epoxiconazole, organic oil oil), oxadixyl, oxazinylazole, oxathiapiprolin, oxycarboxin, oxine-copper, oxytetracycline, oximidazole Oxpoconazole-fumarate, oxolinic acid, copper dioctanoate, octhilinone, ofurace, orysastrobin, o-phenylphenol (o-phenylphenol), kasugamycin (kasugamycin), tetrachlordane (captafol), carpropamid (carpropamid), befendazim (carbendazim), carboxin (carboxin), carboxin (carvone), Candida oleophila, Candida saitoana, quinoxyfen, quinofumelin, chinomethionat, captan , quinconazole, quintozene, guazatine, cufraneb, coumethoxystrobin, coumoxystrobin, Neurospora (Gliocradium catenulatum), Cryptococcus albidus, kresoxim-methyl, clozylacon, Clonostachys rosea, chlozolinate, chloroquine Chloroinconazide, chlorothalonil, chloroneb, Chaetomium cupreum, Coniothyrium minitans, cyazofamid, etiocarb (diethofencarb), diclocymet, dichlofluanid, dichlobentiazox, diclomezine, dicloran, dichlorophen, nitrile dithianon, diniconazole, diniconazole-M, zineb, dinocap, dipymetitrone, diphenylamine ), difenoconazole, cyflufenamid, diflumetorim, cyproconazole, cyprodinil, simeconazole, dimethirimol ), dimethyl disulfide, dimethomorph, cymoxanil, dimoxystrobin, Pseudozyma flocculosa, Pseudomonas aureus ( Pseudomonas aureofaciens), Pseudomonas chlororaphis, Pseudomonas syringae, Pseudomonas fluorescens, Pseudomonas rhodesiae, ziram ), silthiofam, Zucchini yellow mosaic virus-WK, streptomycin, Streptomyces griseoviridis, Streptomyces lygicus , spiroxamine, sedaxane, seboctylamine, zoxamide, solatenol, dazomet, dazomet Talaromyces flavus, tiadinil, thiabendazole, thiram, thiophanate, thiophanate-methyl, thiophanate (thifluzamide), thiram, tecnazene, tecloftalam, tetraconazole, debacarb, tebuconazole, isobutoxyquin Tebufloquin, terbinafine, dodine, dodemorph, triadimenol, triadimefon, triazoxide, salicylus Trichoderma asperellum, Trichoderma atroviride, Trichoderma gamsii, Trichoderma stromaticum, Trichoderma harzianum, Trichoderma viride, Trichoderma virens, Trichoderma polysporum, Trichoderma lignorum, tricyclazole ), triticonazole, tridemorph, triflumizole, trifloxystrobin, triforine, tolylfluanid, tolclofos-methyl , tolnifanide, tolprocarb, nabam, streptomycin, naftifine, nitrapyrin, nitrothal- isopropyl), nuarimol, copper nonyl phenol sulphonate, Paenibacillus polymyxa, Barkholderia cepacia, Bacillus amyloliquefaciens , Bacillus simplex, Bacillus subtilis, Bacillus pumilus, Bacillus licheniformis, harpin protein, Variovorax paradoxus, V. Validamycin, valifenalate, Pantoea agglomerans, picarbutrazox, bixafen, picoxystrobin, Pythium oligandrum, pydiflumetofen, bitertanol, binapacryl, hinokitiol, non-pathogenic bacterial soft rot fungus (non- pathogenic Erwinia carotovora), non-pathogenic Rhizobium vitis, biphenyl, piperalin, hymexazol, pyraoxystrobin, pyraclostrobin ), must remember pyraziflumid, pyrazophos, pyraproponil, pyramidostrobin, pyriofenone, pyrisoxazole, must pyridachlometyl, pyrifenox, pyributicarb, pyribencarb, pyrimethanil, pyroquilon, vinclozolin, thiram Iron (ferbam), famoxadone, phenazine oxide, fenamidone, fenaminstrobin, fenarimol, fenoxanil, Ferimzone, fenpiclonil, fenpicoxamid, fenpyrazamine, fenbuconazole, fenfuram, fenpyridin (fenpropidin), fenpropimorph, fenhexamid, folpet, phthalide, Fusarium oxysporum, bupirimate, wheat fuberidazole, blasticidin-S, furametpyr, furalaxyl, furancarboxylic acid, fluazinam, fluazinam fluindapyr, fluoxastrobin, fluoxapiprolin, fluoxytioconazole, fluopicolide, fluopimomide, fluopicolide (fluopyram), fluoroimide, fluxapyroxad, fluquinconazole, furconazole, furconazole-cis, fludioxonil, fludioxonil flusilazole, flusulfamide, flutianil, flutolanil, flutriafol, flufenoxadiazam, flufenoxystrobin , flubeneteram, flumetylsulforim, flumetover, flumorph, Phlebiopsis gigantea, proquinazid, poker prochloraz, procymidone, prothiocarb, prothioconazole, bronopol, propamocarb-hydrochloride, propiconazole, methyl propineb, probenazole, bromuconazole, flometoquin, florylpicoxamid, hexaconazole, benalaxyl, benalaxyl -M (benalaxyl-M), benodanil, benomyl, pefurazoate, penconazole, pencycuron, benzovindiflupyr ), benthiazole, benthiavalicarb-isopropyl, penthiopyrad, penflufen, boscalid, fosetyl (including aluminum , calcium, and sodium salts), polyoxin, polycarbamate, Bordeaux mixture, mancopper, mancozeb, manganese Mandipropamid, mandestrobin, maneb, myclobutanil, Mitsuaria chitosanitabida, mineral oil, mildiomycin ), methasulfocarb, metam, metalaxyl, metalaxyl-M, metarylpicoxamid, metiram, meteram metyltetraprole, metconazole, metominostrobin, metrafenone, mepanipyrim, mefentrifluconazole, meptyldinocap, Mepronil, iodocarb, laminarin, phosphorous acid and salts, copper oxychloride, silver, copper (II) acetate (copper (II) acetate, cuprous oxide, copper hydroxide, potassium bicarbonate, sodium bicarbonate, sulfur, oxyquinoline sulfate ), copper sulfate (copper sulfate), (3,4-dichloroisothiazol-5-yl)methyl 4-(tertiary butyl) benzoate ((3,4- dichloroisothiazol-5-yl)methyl 4-(tert-butyl)benzoate) (IUPAC name, CAS registration number: 1231214-23-5), UK-2A (coding number), dodecylbenzene sulfonate copper bisethylenediamine [II] salt ( dodecylbenzenesulfonic acid bisethylenediamine copper[II] complex salt (DBEDC), triphenyltin acetate (TPTA), triphenyltin chloride (TPTC), triphenyltin hydroxide (TPTH) .

The following are known insecticides (insecticidal active ingredient), acaricides (acaricidal active ingredient), nematicides (nematicidal active ingredient), and synergist compounds that can be used as mixtures or in combination (Synergizing active ingredient) Non-limiting examples.

Insecticidal active ingredients, acaricidal active ingredients, nematicidal active ingredients, and synergistic active ingredients: Acrinathrin, azadirachtin, azamethiphos, acynonapyr, azinphos-ethyl, azinphos-methyl -methyl), acequinocyl, acetamiprid, acetoprole, azocyclotin, azocyclotin, abamectin, dipropylene Afidopyropen, afoxolaner, amidoflumet, amitraz, alanycarb, aldicarb, aldoxycarb, alenin (allethrin) [including d-cis-trans-form and d-trans-form (d-cis-trans-form and d-trans-form)], isazophos, isamidofos, isocarbophos, isoxathion, isocycloseram, isofenphos-methyl, isoprocarb, epsilon-metofluthrin ), epsilon-momfluorothrin, ivermectin, imicyafos, imidacloprid, imiprothrin, indoxacarb, Yihua Esfenvalerate, ethiofencarb, ethion, ethiprole, ethylene dibromide, etoxazole, etofenprox, ethiprox Ethoprophos, etrimfos, emamectin, emamectin benzoate, endosulfan, empenthrin, oxazosulfyl , oxamyl, oxydemeton-methyl, oxydeprofos, omethoate, nuclear polyhedrosis virus, cadusafos, kappa -kappa-tefluthrin, kappa-bifenthrin, karanjin, cartap, granulosis virus, carbaryl, butyl Carbosulfan, carbofuran, gamma-BHC, xylylcarb, quinalphos, kinoprene, chinomethionat ), enterovirus, coumaphos, cryolite, clothianidin, clofentezine, chromafenozide, chlorantraniliprole ), chlorethoxyfos, chlordane, chloropicrin, chlorpyrifos, chlorpyrifos-methyl, chlorfenapyr, chlorpyrifos (chlorfenvinphos), chlorfluazuron, chlormephos, chloroprallethrin, entomopoxvirus, iridovirus, cyazypyr, cyanide Cyanophos, diafenthiuron, diamidafos, cyantraniliprole, cyetpyrafen, dienochlor, cyenopyrafen, cyetpyrafen (dioxabenzofos), diofenolan, sigmavirus, cyclaniliprole, cycloxaprid, dicrotophos, dichlofenthion, cyclobutrifluram, cycloprothrin, dichlorvos, dichloromezotiaz, dicofol, dicyclanil, dichlorvos disulfoton), dinotefuran, dinobuton, cyhalodiamide, cyhalothrin [including gamma-form and lambda-form] , cyphenothrin [including (1R)-trans-form ((1R)-trans-form)], cyfluthrin [including beta-form], diflubenzuron , cyflumetofen, diflovidazin, cyproflanilide, cyhexatin, cypermethrin [including alpha-form, beta-form ( beta-form), thetap-form, and zeta-form], dimpropyridaz, dimethyl-2,2,2-trichloro-1-hydroxyethylphosphine dimethyl-2,2,2-trichloro-1-hydroxyethyl phosphonate (DEP), dimethylvinphos, dimethoate, dimefluthrin, jasmone ( jasmone), cis-jasmone, jasmonic acid, methyl jasmonate, silafluofen, cyromazine, Steinernema Steinernema carpocapsae, Steinernema kushidai, Steinernema glaseri, spidoxamat, spinetoram, spinosad, spinosad Spirodiclofen, spirotetramat, spiropidion, spiromesifen, sulcofuron-sodium, sulfluramid, sulfoxaflor , sulfotep, diazinon, thiacloprid, thiamethoxam, tioxazafen, thiodicarb, thiocyclam , thiosultap, thionazin, thiofanox, thiometon, tyclopyrazoflor, tetrachlorantraniliprole, lebenzon (tetrachlorvinphos), tetradifon, tetraniliprole, tetramethylfluthrin, tetramethrin, tebupirimfos, tebufenozide , tebufenpyrad, tefluthrin, teflubenzuron, demeton-S-methyl, temephos, deltamethrin ), terbufos, tralomethrin, transfluthrin, triazamate, triazophos, trichlorfon, Trichoderma asperellum ), Trichoderma harzianum, triflumuron, triflumezopyrim, trimethacarb, tolfenpyrad, naled, nicotine ( nicotine), nicofluprole, nitenpyram, nemadectin, Denso virus, novaluron, novifluron, light Paecilomyces lilacinus, Barkholderia cepacia, Barkholderia rinojensis, Verticillium lecanii, hydroprene, Nishizawa Pasteuria nishizawae, Pasteuria penetrans, Bacillus thuringiensis, entomotoxins produced by Bacillus thuringiensis, Bacillus thuringiensis Ayazawa Bacillus thuringiensis subsp. Aizawai, Bacillus thuringiensis subsp. Israelensis, Bacillus thuringiensis subsp. Kurstaki, Bacillus thuringiensis subsp. Kurstaki, Bacillus thuringiensis subsp. subsp. Tenebrionis), Bacillus popilliae, Bacillus licheniformis, vamidothion, parathion, parathion-methyl, parathion (halfenprox), halofenozide, bioallethrin, bioallethrin S-cyclopentenyl, bioresmethrin, bis-(2-chloro- 1-methylethyl)ether (bis-(2-chloro-1-methylethyl)ether (DCIP), bistrifluron, hydramethylnon, bifenazate, bifenin ( bifenthrin), pyflubumide, piperonyl butoxide, pymetrozine, pyraclofos, pyrafluprole, pyridaphenthion, bifenthrin pyridaben, pyridalyl, pyrifluquinazon, pyriprole, pyriproxyfen, pirimicarb, pyrimidifen ), pyriminostrobin, pirimiphos-methyl, pyrethrine, famphur, fipronil, fenazaquin, fenazaquin (fenamiphos), fenitrothion, fenoxycarb, fenothiocarb, phenothrin [including (1R)-trans-form ((1R)-trans-form)] ), fenobucarb, fenthion, phenthoate, fenvalerate, fenpyroximate, fenbutatin oxide, fenpropathrin ), fonofos, sulfuryl fluoride, butocarboxim, butoxycarboxim, buprofezin, furathiocarb, prallethrin , fluacrypyrim, fluazaindolizine, fluazuron, fluensulfone, fluopyram, sodium fluoroacetate, Fluxametamide, flucycloxuron, flucythrinate, flusulfamide, fluthrin, fluvalinate [including tau-form )], flupyradifurone, flupyrazofos, flupyrimin, flufiprole, flufenerim, flufenoxystrobin, flufenoxuron, fluhexafon, flubendiamide, flupentiofenox, flumethrin, fluralaner, flurimfen, Prothiofos, protrifenbute, flonicamid, propaphos, propargite, prohydrojasmon, profenofos, buprofen broflanilide, profluthrin, propetamphos, propoxur, flometoquin, bromopropylate, hexythiazox, hexafuron hexaflumuron), Paecilomyces tenuipes, Paecilomyces fumosoroceus, Paecilomyces lilacinus, heptafluthrin, heptenophos, hexafluthrin permethrin, benclothiaz, benzpyrimoxan, bensultap, benzoximate, bendiocarb, benfuracarb, chlamydospora Pochonia chlamydosporia, Beauveria tenella, Beauveria bassiana, Beauveria brongniartii, phoxim, eubienne (phosalone), fosthiazate, fosthietan, phosphamidon, phosmet, polynamycin complex (polynactins), formetanate, fosthietan ( phorate), machine oil, malathion, milbemectin, mecarbam, mesulfenfos, methomyl, metaldehyde , metaflumizone, methamidophos, metham, methiocarb, methidathion, methyl isothiocyanate, methyl bromide ), methoxychlor, methoxyfenozide, methothrin, metofluthrin, methoprene, metolcarb, methophos (mevinphos), meperfluthrin, Monacrosporium phymatophagum, Monacrosporium phymatophagum, monocrotophos, momfluorothrin, Trichoderma harveyi Trichoderma harzianum), litlure-A (litlure-A), litlure-B (litlure-B), aluminum phosphide, zinc phosphide, phosphine, lufenron (lufenuron), rescalure, resmethrin, lepimectin, rotenone, cytoplasmic polyhedrosis virus, fenbu Fenbutatin oxide, calcium cyanide, organotins, nicotine-sulfate, (Z)-11-tetradecenyl=acetate ((Z)- 11-tetradecenyl=acetate), (Z)-11-hexadecenal ((Z)-11-hexadecenal), (Z)-11-hexadecenyl=acetate ((Z)-11- hexadecenyl=acetate), (Z)-9,12-tetradecadienyl=acetate ((Z)-9,12-tetradecadienyl=acetate), (Z)-9-tetradecadien-1- Alcohol ((Z)-9-tetradecen-1-ol), (Z,E)-9,11-tetradecadienyl=acetate ((Z,E)-9,11-tetradecadienyl=acetate) , (Z,E)-9,12-tetradecadienyl=acetate ((Z,E)-9,12-tetradecadienyl=acetate), 1,1,1-trichloro-2,2- Bis (4-chlorophenyl)ethane (1,1,1-trichloro-2,2-bis (4-chlorophenyl)ethane. DDT), 1,3-dichloropropene (1,3-dichloropropene), 2 ,4-dichloro-5-{2-[4-(trifluoromethyl)phenyl]ethoxy}phenyl 2,2,2-trifluoroethyl styrene (2,4-dichloro-5- {2-[4-(trifluoromethyl)phenyl]ethoxy}phenyl 2,2,2-trifluoroethyl sulfoxide) (IUPAC name, CAS registration number: 1472052-11-1), 2,4-dimethyl-5-[6 -(Trifluoromethylthio)hexyloxy]phenyl-2,2,2-trifluoroethylterinoxide (2,4-dimethyl-5-[6-(trifluoromethylthio)hexyloxy]phenyl-2,2, 2-trifluoroethyl sulfoxide) (IUPAC name, CAS registration number: 1472050-34-2), 2-{2-fluoro-4-methyl-5-[(2,2,2-trifluoroethyl)sulfenyl methyl]phenoxy}-5-(trifluoromethyl)pyridine(2-{2-fluoro-4-methyl-5-[(2,2,2-trifluoroethyl)sulfinyl]phenoxy}-5-(trifluoromethyl) pyridine) (IUPAC name, CAS registration number: 1448758-62-0), 3-chloro-2-{2-fluoro-4-methyl-5-[(2,2,2-trifluoroethyl)sulfen acyl)]phenoxy}-5-(trifluoromethyl)pyridine(3-chloro-2-{2-fluoro-4-methyl-5-[(2,2,2-trifluoroethyl)sulfinyl]phenoxy} -5-(trifluoromethyl)pyridine) (IUPAC name, CAS registration number: 1448761-28-1), 4,6-dinitro-o-cresol (DNOC), 4 -Fluoro-2-methyl-5-(5,5-dimethylhexyloxy]phenyl 2,2,2-trifluoroethylsterine (4-fluoro-2-methyl-5-(5, 5-dimethylhexyloxy]phenyl 2,2,2-trifluoroethyl sulfoxide) (IUPAC name, CAS registration number: 1472047-71-4), Bt protein (Cry1Ab, Cry1Ac, Cry1Fa, Cry2Ab, mCry3A, Cry3Ab, Cry3Bb, Cry34/35Ab1), methyl eugenol, 4-(p-acetoxyphenyl)-2-butanone, (Z)-10 -Tetradecenyl=acetate ((Z)-10-tetradecenyl=acetate), (E,Z)-4,10-tetradecenyl=acetate ((E,Z)-4 ,10-tetradecadienyl=acetate), (Z)-8-dodecenyl=acetate ((Z)-8-dodecenyl=acetate), (Z)-11-tetradecadienyl=acetate ((Z)-11-tetradecenyl=acetate), (Z)-13-eicosen-10-one ((Z)-13-eicosen-10-one), 14-methyl-1-octadecene (14-methyl-1-octadecene), AKD-1193 (coding number), BCS-AA10147 (coding number), CL900167 (coding number), O,O-diethyl-O-[4-(dimethylamine Sulfamoyl)phenyl]-phosphorothioate (O,O-diethyl-O-[4-(dimethylsulfamoyl)phenyl]-phosphorothionate, DSP), O-ethyl-O-4-(nitrophenyl) Phenyl phosphonothioate (O-ethyl-O-4-(nitrophenyl)phenyl phosphonothioate, EPN), RU15525 (coding number), XMC, Z-13-eicosen-10-one (Z-13-eicosen -10-one), ZXI8901 (coding number), F4260 (coding number).

The following compounds are non-limiting examples of known herbicidal compounds, herbicidal active ingredients, and plant growth regulators that may be used as mixtures or combinations.

Herbicide compounds and herbicidal active ingredients Ioxynil (including lithium salt, sodium salt, and octanoate), aclonifen, acrolein, azafenidin, acifluorfen (including sodium salt of salt), azimsulfuron, asulam, acetochlor, atrazine, anisiflupurin, anilofos, Amicarbazone, amidosulfuron, amitrole, aminocyclopyrachlor, aminopyralid, amiprofos-methyl , ametryn, Araujia mosaic virus, alachlor, Alternaria destruens, alloxydim (including salts such as sodium salt), ring ancymidol, isouron, isoxachlortole, isoxaflutole, isoxaben, isodecyl alcohol ethoxylate , isoproturon (isoproturon), ipfencarbazone (ipfencarbazone), imazaquin (imazaquin), imazapic (including salts such as amine salts), imazapyr (including salts such as isopropyl salts of amine salts), imazamethabenz, imazamethabenz-methyl, imazamox, imazethapyr, imazosulfuron, indoxylfluidine ( indaziflam), indanofan, eglinazine-ethyl, esprocarb, ethametsulfuron-methyl, ethalfluralin, sulfothiane Ethidimuron, ethoxysulfuron, ethoxyfen, ethoxyfen-ethyl, ethofumesate, etobenzanid ), epyrifenacil, endothal-disodium, oxadiazon, oxadiargyl, oxaziclomefone, oxasulfuron , oxyfluorfen, oryzalin, Obuda pepper virus, orthosulfamuron, orbencarb, oleic acid, benzophenone cafenstrole, caprylic acid, capric acid, carfentrazone-ethyl, karbutilate, carbetamide, quizalofop ), quizalofop-ethyl, quizalofop-P-ethyl, quizalofop-P-tefuryl, Xanthomonas campestris), quinoclamine, quinclorac, quinmerac, citric acid, cumyluron, clacyfos, glyphosate (glyphosate) (including sodium salt, potassium salt, amine salt, propylamine salt (propylamine salt), isopropylamine salt (isopropylamine salt), ammonium salt, isopropylammonium salt (isopropylammonium salt), guanidine salt ), monoethanolamine salt, choline salt, BAPMA (N,N-bis-(aminopropyl)methylamine) salt ), dimethylamine salt, and salts of trimesium salt), glufosinate (including salts such as amine salts or sodium salts), glufosinate-P ( glufosinate-P), glufosinate-P-sodium, clethodim, clodinafop, clodinafop-propargyl, clethodim ( clopyralid) (including salts such as monoethanolamine salt), clomazone, chlomethoxyfen, clomeprop, cloransulam-methyl, cloransulam-methyl Chloramben, chloridazon, chlorimuron, chlorimuron-ethyl, chlorsulfuron, dichlorthal-dimethyl, Chlorthiamid, chlorphthalim, chlorflurenol-methyl, chlorpropham, chlorbromuron, chloroxuron, chlorotoluron , ketospiradox (including salts such as sodium salt, calcium salt, or amine salt), Colletotrichum orbiculare, Colletotrichum gloeosporioides, curved type Colletotrichum truncatum, fungal plant pathogen (Chondrostercum purpureum), saflufenacil, sarmentine, cyanazine, cyanamide, diuron , diethatyl-ethyl, dioxopyritrione, dicamba (including amine salts, diethylamine salts, isopropylamine salts, diglycolamine salt, dimethylammonium salt, glycolamine salt, isopropylammonium salt, auramine salt, potassium salt, trolamine salt, BAPMA (N,N-bis-(aminopropyl) )Methylamine) salt, choline salt, sodium salt, and lithium salt or ester such as methyl ester), cycloate, cycloxydim, diclosulam, cyclosulam cyclosulfamuron, cyclopyranil, cyclopyrimorate, dichlobenil, diclofop, diclofop-P-methyl methyl), diclofop-methyl, dichlorprop, dichlorprop-P (including dimethyl ammonium salt, potassium salt, sodium salt, and choline Salts of salts; and for example, esters such as butoxyethyl ester, 2-ethylhexyl ester, isooctyl ester, methyl ester), diquat, dibromide, Dithiopyr, siduron, dinitramine, cinidon-ethyl, cinosulfuron, dinoseb (including acetic acid salt), dinoterb, cyhalofop, cyhalofop-butyl, cypyrafluone, diphenamid, cyhalofop (difenzoquat), diflufenican, diflufenzopyr, simazine, dimesulfazet, dimethachlor, dimethamemetryn, dimethachlor dimethenamid), dimethenamid-P, simetryn, dimepiperate, dimefuron, Pseudomonas fluorescens, heptafen (cinmethylin), swep, sulcotrione, sulfentrazone, sulfosate, sulfosulfuron, sulfometuron -methyl), sethoxydim, Scelerothinia minor, terbacil, daimuron, thaxtomin A, tobacco green mosaic virus ( Tobacco mild green mosaic tobamoviruses), Tobacco rattle virus, dalapon, thiazopyr, tiafenacil, thiencarbazone (including e.g. Sodium salt, and methyl ester), tiocarbazil, thiobencarb, thidiazimin, thidiazuron, thifensulfuron, thifensulfuron-methyl (thifensulfuron-methyl), desmedipham, desmetryne, tetflupyrolimet, thenylchlor, tebutam, tebuthiuron, desal Tepraloxydim, tefuryltrione, terbuthylazine, terbutryn, terbumeton, tembotrione, topramezone, terbumeton Tralkoxydim, triaziflam, triasulfuron, triafamone, tri-allate, trietazine, triclopyr, Triclopyr-butotyl, triclopyr-triethylammonium, tritosulfuron, tripyrasulfone, triethylammonium (trifludimoxazin), triflusulfuron-methyl, trifluralin, trifloxysulfuron (including salts such as sodium salt), tribenuron-methyl methyl), tolpyralate, naptalam (including salts such as sodium salt), naproanilide, napropamide, napropamide-M, Nicosulfuron (nicosulfuron), lactic acid, neburon, norflurazon, Burkholderia rinojensis, vernolate, paraquat, Paraquat dichloride, halauxifen, halauxifen-benzyl, halauxifen-methyl, haloxyfop, halauxifen -P (haloxyfop-P), haloxyfop-etotyl, haloxyfop-P-methyl, halosafen, haloxyfop-etotyl halosulfuron-methyl), bixlozone, picloram (including salts of dichlorammonium salt and triethanolamine salt), picolinafen, bicyclopyrone ), bispyribac-sodium, pinoxaden, bipyrazone, bifenox, piperophos, pyraclonil ), pyrasulfotole, pyrazoxyfen, pyrazosulfuron-ethyl, pyrazolynate, bilanafos, pyraflufen , pyraflufen-ethyl, pyridafol, pyrithiobac-sodium, pyridate, pyriftalid, barnyardgrass Pyributicarb, pyribenzoxim, pyrimisulfan, pyriminobac-methyl, pyroxasulfone, pyroxsulam , Phytophthora palmivora, phenisopham, fenuron, fenoxasulfone, fenoxaprop (including, for example, methyl ester, ethyl ester, isopropyl ester ), fenoxaprop-P (including, for example, methyl ester, ethyl ester, isopropyl ester), fenquinotrione, fenthiaprop-ethyl, fenthiaprop-ethyl Fentrazamide, fenpyrazone, phenmedipham, Phoma chenopodicola, Phoma herbarum, Phoma macrostoma, fentrazamide butachlor, butafenacil, butamifos, butylate, Puccinia canaliculata, Puccinia thlaspeos, butylene butenachlor, butralin, butroxydim, flazasulfuron, flamprop (including, for example, methyl ester, ethyl ester, isopropyl ester), flamprop -M (flamprop-M) (including, for example, methyl ester, ethyl ester, isopropyl ester), primisulfuron-methyl, primisulfuron-methyl, fluazifop- butyl), fluazifop-P (fluazifop-P), fluazifop-P-butyl, fluazolate, fluometuron, fluazifop-P-butyl Ether-ethyl (fluoroglycofen-ethyl), flucarbazone-sodium, fluchloralin, flucetosulfuron, fluthiacet-methyl, fluorine flupyrsulfuron-methyl (including salts such as sodium salt, calcium salt, and amine salt), flufenacet, flufenpyr-ethyl, tetrafluoropropionic acid (flupropanate) (including sodium salt), flupoxame, flumioxazin, flumiclorac-pentyl, flumetsulam, flumetsulam ( fluridone), flurtamone, fluroxypyr (including esters such as butylmethyl ester, isooctyl ester (meptyl), and esters such as sodium salt, calcium salt, and amine salt salt), flurochloridone, pretilachlor, procarbazone (including salts such as sodium salt), prodiamine, prosulfuron , prosulfocarb, propaquizafop, propachlor, propazine, propanil, propyzamide, propisochlor, Propyrisulfuron (propyrisulfuron), propham (propham), profluazol (profluazol), prohexadione-calcium, propoxycarbazone, profluron-sodium salt ( propoxycarbazone-sodium), profoxydim, bromacil, brompyrazon, prometryn, prometon, bromoxynil (including, for example, such as Butyrate, caprylate, and enanthate ester), bromofenoxim, bromobutide, florasulam, florpyrauxifen, florpyrauxifen-benzyl, hexazinone, pethoxamid, benazolin, benazolin-ethyl, benazolin-ethyl, penoxsulam), Pepino mosaic virus, heptamaloxyloglucan, beflubutamid, beflubutamid-M, pebulate ), pelargonic acid, bencarbazone, benquitrione, benzfendizone, bensulide, bensulfuron, bensulon -bensulfuron-methyl, benzobicyclon, benzofenap, bentazone, pentanochlor, pendimethalin, pentoxazone ), benfluralin, benfuresate, fosamine, fomesafen, foramsulfuron, forchlorfenuron, meclopropionic acid ( mecoprop) (including salts such as sodium, potassium, isopropylamine, triethanolamine, dimethylamine, glycolamine, triethanolamine, and choline, and, for example, ethadyl ester, 2-ethylhexyl ester, isooctyl ester, and methyl ester), mecoprop-P-potassium, mesosulfuron (mesosulfuron) (including, for example, such as methyl ester ester), mesotrione, metazachlor, metazosulfuron, methabenzthiazuron, metamitron, meclopropionic acid metamifop), metam (including salts such as sodium salt), disodium methyl arsonate (DSMA), methiozolin, methyldymuron, methoxy Metoxuron, metosulam, metsulfuron-methyl, metobromuron, metobenzuron, metolachlor, metsulfuron-methyl metribuzin), mepiquat chloride, mefenacet, monosulfuron (including methyl ester, ethyl ester, and isopropyl ester), monolinuron, Daode Molinate, iodosulfuron-methyl, iodosulfuron-methyl-sodium, iofensulfuron-sodium, iofensulfuron-sodium ), lactofen, lancotrione, linuron, rimisoxafen, rimsulfuron, lenacil, 2,2,2-trichloroacetic acid (TCA) (including salts such as sodium salt, calcium salt, and amine salt), 2,3,6-trichlorobenzoic acid (2, 3,6-trichlorobenzoic acid, 2,3,6-TBA), 2,4,5-trichlorophenoxyacetic acid (2,4,5-trichlorophenoxyacetic acid, 2,4,5-T), 2,4 -Dichlorophenoxyacetic acid (2,4-D) (including amine salt, diethylamine salt, triethanolamine salt, isopropylamine salt, dimethylammonium salt, glycol Amine salt, dodecyl ammonium salt, heptyl ammonium salt, tetradecyl ammonium salt, triethylammonium salt, ginseng (2-hydroxypropyl) ammonium salt, triethanolamine salt, choline salt, sodium salt, And salts of lithium salts; or such as butoxyethyl ester, 2-butoxypropyl ester, 2-ethylhexyl ester, methyl ester, ethyl ester, butyl ester, isobutyl ester, octyl ester, amyl ester, propyl ester , isooctyl ester, isopropyl ester, meptyl ester, and furfuryl ester), 2,4-dichlorophenoxybutyric acid (2,4-DB) (including Salts such as amine salts, diethylamine salts, triethanolamine salts, isopropylamine salts, dimethylammonium salts, choline salts, sodium salts, and lithium salts; or esters such as isooctyl ester), 2- Amino-3-chloro-1,4-naphthoquinone (ACN), 2-methyl-4-chlorophenoxyacetic acid (2-methyl-4- chlorophenoxyacetic acid, MCPA) (including salts such as sodium salt, dimethylammonium salt, choline salt; and esters such as 2-ethylhexyl ester, isooctyl ester, and ethyl ester), 2-methyl-4- Chlorophenoxybutyric acid (2-methyl-4-chlorophenoxybutyric acid, MCPB) (including, for example, sodium salt, and ethyl ester), 4-(2,4-dichlorophenoxybutyric acid) (4-(2, 4-dichlorophenoxy) butyric acid, 2,4-DB), 4,6-dinitro-O-cresol (DNOC) (including amine salts and sodium salts), ( 5S)-3-(3,5-difluorophenyl)-N-[rel-(3R,5R)-5-(trifluoromethylsulfonylaminemethyl)tetrahydrofuran-3-yl]-5 -Vinyl-4H-isoethazole-5-methamide ((5S)-3-(3,5-difluorophenyl)-N-[rel-(3R,5R)-5- (trifluoromethylsulfonylcarbamoyl) tetrahydrofuran-3-yl]-5-vinyl-4H-isoxazole-5-carboxyamide) (IUPAC name; CAS registration number: 2266183-40-6) (WO2018/228986, WO2020/114934), N4-(2 ,6-difluorophenyl)-6-(1-fluoro-1-methyl-ethyl)-1,3,5-triazine-2,4-diamine (N4-(2,6-difluorophenyl) -6-(1-fluoro-1-methyl-ethyl)-1,3,5-triazine-2,4-diamine) (IUPAC name; CAS registration number: 1606999-43-2) (WO2014/064094, WO2015 /162164), (5S)-3-(3,5-difluorophenyl)-N-[(3R)-5-(methylsulfonylaminemethyl)-2,3-dihydrofuran- 3-yl]-5-vinyl-4H-isoethazole-5-methamide ((5S)-3-(3,5-difluorophenyl)-N-[(3R)-5-(methylsulfonylcarbamoyl)-2 ,3-dihydrofuran-3-yl]-5-vinyl-4H-isoxazole-5-carboxyamide) (IUPAC name; CAS registration number: 2266190-06-9) (WO2018/228986, WO2020/114934), (5R)- 3-(3,5-Difluorophenyl)-5-methyl-N-[rel-(3R,5R)-5-(methylsulfonylaminemethyl)tetrahydrofuran-3-yl]-4H -Isoethazole-5-carbamoyl((5R)-3-(3,5-difluorophenyl) -5-methyl-N-[rel-(3R,5R)-5-(methylsulfonylcarbamoyl) tetrahydrofuran-3-yl ]-4H-isoxazole-5-carboxyamide) (IUPAC name; CAS registration number: 2266164-36-5) (WO2018/228986, WO2020/114934), (5R)-3-(3,5-difluorophenyl) -N-[(3R)-5-(Methoxyaminemethyl)-2,3-dihydrofuran-3-yl]-5-methyl-4H-isoethazole-5-methamide ( (5R)-3-(3,5-difluorophenyl)-N-[(3R)-5-(methoxycarbamoyl)-2,3-dihydrofuran-3-yl]-5-methyl-4H-isoxazole-5-carboxyamide) (IUPAC name; CAS registration number: 2266170-31-2) (WO2018/228986, WO2020/114934), 2-[2-(3,4-dimethoxyphenyl)-6-methyl-3-side Oxy-pyridazine-4-carbonyl]cyclohexane-1,3-dione (2-[2-(3,4-dimethoxyphenyl)-6-methyl-3-oxo-pyridazine-4-carbonyl] cyclohexane- 1,3-dione) (IUPAC name; CAS registration number: 2138855-12-4) (WO2017/178582, WO2018/015476), 4-hydroxy-1-methyl-3-[4-(trifluoromethyl) -2-pyridyl]imidazolidin-2-one (4-hydroxy-1-methyl-3-[4-(trifluoromethyl)-2-pyridyl] imidazolidin-2-one) (IUPAC name; CAS registration number: 1708087 -22-2) (WO2015/059262, WO2018/015476), 6-(1-fluorocyclopentyl)-N4-(2,3,5,6-tetrafluorophenyl)-1,3,5-tri Triazine-2,4-diamine (6-(1-fluorocyclopentyl)-N4-(2,3,5,6-tetrafluorophenyl)-1,3,5-triazine-2,4-diamine) (IUPAC name; CAS Registration number: 1820807-75-7) (WO2015/162164), 6-(1-fluoro-1-methyl-ethyl)-N4-(2,3,5,6-tetrafluorophenyl)-1, 3,5-Triazine-2,4-diamine (6-(1-fluoro-1-methyl-ethyl)-N4-(2,3,5,6-tetrafluorophenyl)-1,3,5-triazine - 2,4-diamine) (IUPAC name; CAS registration number: 1606999-21-6) (WO2014/064094, WO2015/162164), (5S)-3-(3-fluoro-5-methyl-phenyl)- N-[rel-(3R,5R)-5-(methoxyaminomethyl)tetrahydrofuran-3-yl]-5-vinyl-4H-isoethyl-5-methamide ((5S)- 3-(3-fluoro-5-methyl-phenyl)-N-[rel-(3R,5R)-5-(methoxycarbamoyl)tetrahydrofuran-3-yl]-5-vinyl-4H-isoxazole-5-carboxyamide) ( IUPAC name; CAS registration number: 2266292-43-5) (WO2018/228986, WO2020/114934), 6-(1-methylcyclobutyl)-N4-(2,3,5,6-tetrafluorophenyl )-1,3,5-triazine-4,4-diamine (6-(1-methylcyclobutyl)-N4-(2,3,5,6-tetrafluorophenyl)-1,3,5-triazine-4, 4-diamine) (IUPAC name; CAS registration number: 1607001-97-7) (WO2014/064094, WO2015/162164), AE-F-150944 (coding number), F9960 (coding number), IR-6396 (coding number ), MCPA-thioethyl, NC-656 (coding number), SYP-298 (coding number), SYP-300 (coding number), S-ethyldipropylthiocarbamate (S-ethyldipropylthiocarbamate, EPTC), S-metolachlor, S-9750 (coding number), MSMA, HW-02 (coding number), S-523 (coding number), and SL-1201 (coding number).

plant growth regulator 1-Naphthylacetamide, 1-methylcyclopropene, 1,3-diphenylurea, 2,3,5-triiodobenzene Formic acid (2,3,5-triiodobenzoic acid), 2-methyl-4-chlorophenoxybutyric acid (MCPB) [including, for example, sodium salt, and ethyl ester], 2- (Naphthalene-1-yl)acetamide (2-(naphthalene-1-yl)acetamide), 2,6-diisopropylnaphthalene (2,6-diisopropylnaphthalene), 3-[(6-chloro-4- Phenylquinazoline-2-yl)amino]propane-1-ol (3-[(6-chloro-4-phenylquinazoline-2-yl) amino]propane-1-ol), 4-side oxy- 4-oxo-4-(2-phenylethyl)aminobutyric acid (IUPAC name; CAS registration number: 1083-55-2), 4-chlorophenoxy Acetic acid (4-chlorophenoxyacetic acid, 4-CPA), 5-aminoacetylpropionic acid hydrochloride (5-aminolevulinic acid hydrochloride), 5-(trifluoromethyl)benzo[b]thiophene-2-carboxylic acid Methyl ester (methyl 5-(trifluoromethyl) benzo[b]thiofen-2-carboxylate), AVG (aminoethoxyvinylglycine), n-decyl alcohol (n- decanol), anisiflupurin, aviglycine, ancymidol, abscisic acid, isoprothiolane, inabenfide, indoline Indole acetic acid, indole butyric acid, uniconazole, uniconazole-P, Ecolyst, ethychlozate, beneficial Ethephon, epocholeone, calcium chloride, choline chloride, oxine-sulfate, opabatin (opabactin), kinetin, calcium peroxide, carvone, quinoxaline (quinabactin), calcium formate, cloxyfonac, hydroxyl acid- Potassium salt (cloxyfonac-potassium), cloprop, chlormequat, chlormequat-chloride, chlorpropham, choline, cytokinins ), oxidized glutathione, cyanamide, sodium cyanate, cyclanilide, dichlorprop (including dimethylammonium salts such as , potassium salt, sodium salt, and choline salt; and esters such as butoxyethyl ester, 2-ethylhexyl ester, isooctyl ester, and methyl ester), dichlorprop-P (Including salts such as sodium salt, potassium salt, and dimethyl ammonium salt; and 2-ethylhexyl ester), diquat, diquat dibromide, dikegulac ), gibberellinic acid, gibberellin A4, gibberellin A7, dimethipin, sintofen, jasmone, cis - cis-jasmone, jasmonic acid, methyl jasmonate, streptomycin, calcium polysulfide, daminozide, calcium carbonate ( calcium carbonate), thidiazuron, decan-1-ol, triacontanol, triapenthenol, trinexapac-ethyl, defoliation Phosphorus (tribufos), paclobutrazol, paraffin, bispyribac-sodium, hymexazol, butralin, fluthiacet- methyl), pyraflufen-ethyl, flumetralin, flurprimidol, flurenol, pronitridine, prohydrojasmon ), prohexadione-calcium, heptamaloxyloglucan, 6-benzylaminopurine, pendimethalin, forchlorfenuron, thorn formononetin, maleic hydrazide, mepiquat chloride, mefluidide, lipochitooligosaccharide (such as lipochitooligosaccharide SP104 ( lipochitooligosaccharide SP104)), and calcium sulfate.

The following are non-limiting examples of known protectant compounds that can be used as mixtures or in combination.

Protective agent Isoxadifen, isoxadifen-ethyl, oxabetrinil, octane-1,8-diamine, detoxification Cloquintocet, cloquintcet-mexyl, dietholate, cyometrinil, dichlormid, dicyclonone, cyprosulfamide ), daimuron, 1,8-naphthalic anhydride, fenchlorazole, fenchlorazole-O-ethyl, fenchlorazole Fenclorim, furilazole, fluxofenim, flurazole, benoxacor, metcamifen, mephenate, pyrazole Oxalic acid (mefenpyr), mefenpyr-ethyl, mefenpyr-diethyl, lower alkyl substituted benzoic acid, 2,2 -Dichloro-N-(1,3-dioxopent-2-ylmethyl)-N-(2-propenyl)acetamide (2,2-dichloro-N-(1,3-dioxolan- 2-ylmethyl)-N-(2-propenyl)acetamide, PPG-1292), 2-dichloromethyl-2-methyl-1,3-dihexane (2-dichloromethyl-2-methyl-1,3 -dioxane, MG-191), 3-dichloroacetyl-2,2,5-trimethyl-1,3-oxazolidine (3-dichloroacetyl-2,2,5-trimethyl-1,3- oxazolidine, R-29148), 4-dichloroacetyl-1-oxa-4-azaspiro[4.5]decane, AD- 67), 4-carboxy-3,4-dihydro-2H-1-benzopyran-4-acetic acid (CL -304415, code number), MON4660 (code number), metcamifen, N1,N2-diallyl-N2-dichloroacetylglycinamide (N1,N2-diallyl-N2- dichloroacetylglycineamide) (DKA-24, code number), 1-bromo-4-[(chloromethyl)sulfonyl]benzene, CSB), 1-oxa -4-Azaspiro[4,5]decane-4-carbodithioate 2-propenyl 1-oxa-4-azaspiro[4,5]decane-4-carbodithioate (MG- 838, code number), 3-(dichloroacetyl)-2,2-dimethyl-1,3-oxazolidine (3-(dichloroacetyl)-2,2-dimethyl-1,3-oxazolidine) (R-28725, coding number), R-29148 (coding number), and 1-(dichloroacetyl)azepane (TI-35, coding number).

The following are non-limiting examples of known biopesticides that can be used as mixtures or in combination.

biopesticides Haplothrips brevitubus, Franklinothrips vespiformis, Diglyphus isaea, Encarsia formosa, Amblyseius cucumeris, mealybug Pseudaphycus malinus, Amblyseius womersleyi, Aphidius colemani, Eretmocerus eremicus, Aphidoletes aphidimyza , Amblyseius swirskii, Orius strigicollis, Phytoseiulus persimilis, Amblyseius degenerans, Phytoseiulus persimilis , Orius sauteri, Dacnusa sibirica, Amblyseius californicus, Chrysoperla nipponensis, and Anicetus beneficus ).

The following are non-limiting examples of known agricultural materials that can be used as mixtures or combinations.

agricultural materials Ethylene, hypochlorous acid solution (produced only by electrolysis of hydrochloric acid or potassium chloride aqueous solution), baking soda, vinegar, humus, humic acid, fulvic acid, seaweed extract, polysaccharides , amino acids, microbial materials, functional components derived from animals and plants, microbial metabolites, microbial active materials, soil spreading agents, soil-systemic regulatory materials, soil Soil-water retaining material, and biostimulant.

The following are non-limiting examples of known agricultural fertilizers that can be used as mixtures or in combination.

Fertilizers include inorganic fertilizers and organic fertilizers. Examples include ammonium chloride, ammonium sulfate, ammonium nitrate, ammonium dihydrogen phosphate, urea ammonium nitrate, urea, nitrolime, potassium nitrate, superphosphate, double superphosphate, potassium dihydrogen phosphate, potassium chloride , potassium sulfate, potassium carbonate, potassium silicate, oil meal, fish meal, rice bran, bat guano, and fermented chicken manure.

The plant growth regulator, and/or environmental stress resistance conferring agent, and/or plant disease control agent of the present invention are also suitable for being obtained through newer breeding technologies (such as genetic recombination, genome editing), and artificial hybridization. Plants with properties such as resistance to pests, diseases, and herbicides.

As mentioned above, the plant growth regulator of the present invention containing viable bacteria or strain cultures of the genus Bacillus as an active ingredient can provide materials that contribute to crop production through the combined effect of two methods: by plant growth regulation effects and confer resistance to environmental stress to increase profits, and reduce profit losses through disease control.

The following describes embodiments of the present invention. However, it should be noted that the present invention is not limited to the following embodiments, and various modifications can be made within the technical contents of the present invention. Example 1

(Production Example 1: Production of wet bacteria) The Bacillus genus D747 strain (FERM BP-8234) isolated from the air in Shizuoka Prefecture was cultured on a flat plate medium, and the isolated colony was inoculated into a flask. After shaking culture for 1 day with 20 ml of ordinary meat broth medium (meat extract 1%, proteinaceous 1%, sodium chloride 0.5%) at 27°C and 120 rpm, the resulting culture was inoculated to a medium containing 1% glucose, 2% Soluble starch, 0.5% polypeptone, 1% dry yeast, 1% defatted soybeans, 0.2% KH ₂ PO ₄ , 0.2% sodium chloride, and 0.3% calcium carbonate in 20 L medium (pH 6.0). Bacterial cells were then grown by shaking culture at 27°C, 120 rpm for 3 days, harvested by centrifugation (10,000×g, 15 min), and suspended in sterile water, followed by washing of culture medium components. Repeat this procedure twice to yield wet bacteria (endospore fraction) with a wet weight of approximately 1 kg. The endospore fraction contained 50% by weight of endospores of the genus Bacillus D747 on a dry weight basis. Example 2

(Production Example 2: Production of Powdered Endospores) One kilogram of the endospore portion of Bacillus genus D747 obtained in Production Example 1 was suspended in five liters of distilled water and dried with a spray dryer (NIRO, Japan) (inlet temperature 150℃, outlet temperature 100℃) drying. The dried material from the spray dryer was then crushed and approximately 100 g of endospore dried powder was obtained. The dry powder contains 6×10 ¹¹ cfu/g or more endospores of Bacillus genus D747.

The following is a specific preparation method based on typical embodiments of preparing the agricultural and horticultural composition of the present invention. In the following description, "%" means weight percentage. Example 3

(Preparation Example 1: Wettable powder) The wettable powder is prepared by mixing 10% of the powdery endospores of the Bacillus genus D747 strain obtained in Preparation Example 2 and 1.5% of the sodium salt of the condensate of naphthalene sulfonic acid formalin. , 1.5% polyoxyethylene alkyl aryl, 26% algae, and 61% clay are evenly mixed and crushed. Example 4

(Testing the growth-promoting effect of spraying onto stems and leaves on potatoes) A 1:500 diluted aqueous solution of the wettable powder of the Bacillus genus D747 strain produced according to Example 3 was sprayed onto potatoes (variety: Nishiyutak) that had grown to the seven compound leaf stage in the pot. Spray a sufficient amount of solution three times at intervals of one week. For comparison, the same amount of water was sprayed on potato stems and leaves (control group).

One week after the third spraying, the fresh weight of the above-ground parts of each potato was measured. The dry weight of the underground portion was also measured after placing it in a dryer that had been set to 120°C for 3 days. The weight ratio relative to the control group was calculated using Equation 1 below.

(Formula 1) Weight ratio relative to the control group = (weight of the treatment group/weight of the control group) × 100

The test results are presented in Table 1 below and Figure 1 below. The control group had a fresh weight of 19.2 g/plant in the above-ground parts and a dry weight of 1.17 g/plant in the underground parts. The wettable powder treatment group had a fresh weight of 20.0 g/plant in the above-ground part and a dry weight of 1.68 g/plant in the underground part, showing that both the fresh weight of the above-ground part and the dry weight of the underground part were higher than those of the above-ground part in the control group. fresh weight and dry weight of underground parts. There were no crop diseases or insect pests in the wettable powder treatment group and the control group. The wettable powder of Bacillus sp. D747 strain showed a growth-promoting effect on potatoes.

[Table 1] spray group Fresh weight of aboveground parts (g/plant) Ratio of aboveground fresh weight to control group (%) Dry weight of underground parts (g/plant) Ratio of dry weight of underground part to control group (%) wettable powder 20.0 104 1.68 144 control group 19.2 - 1.17 - [Example 5]

(Testing the growth-promoting effect of irrigation treatment on soybeans) Soybeans (variety: Fukuyutaka) were grown to primary leaves in a 128-hole cell plate filled with seedling soil (Yosaku ^® , manufactured by JCAM AGRI Co., Ltd) Expansion stage (primary leaf development stage). Then, a 1:500 diluted aqueous solution of the wettable powder produced according to Example 3 was applied to the base of the soybeans as an irrigation treatment, using 2.6 ml of the solution for each soybean plant. For comparison, the same amount of water was used for the irrigation treatment of soybeans (control group). On the second day of the irrigation treatment, the plants were transplanted into plastic cups T-600 (Tomei Chemical Industrial Co., Ltd.) filled with soil.

On the 21st day after transplantation, the fresh weight of the aboveground part of each soybean plant was measured. The dry weight of the underground portion was also measured after placing it in a dryer that had been set to 120°C for 2 days. Calculate the weight ratio relative to the control group using Equation 1.

The test results are presented in Table 2 below and Figure 2 below. The control group had a fresh weight of 15.96 g/plant in the above-ground parts and a dry weight of 0.89 g/plant in the underground parts. The wettable powder treatment group had a fresh weight of 17.21 g/plant in the above-ground part and a dry weight of 1.03 g/plant in the underground part, showing that both the fresh weight of the above-ground part and the dry weight of the underground part were higher than those of the above-ground part in the control group. fresh weight and dry weight of underground parts. There were no crop diseases or insect pests in the wettable powder treatment group and the control group. The wettable powder of Bacillus genus D747 strain showed a growth-promoting effect on soybeans.

[Table 2] treatment group Fresh weight of aboveground parts (g/plant) Ratio of aboveground fresh weight to control group (%) Dry weight of underground parts (g/plant) Ratio of dry weight of underground part to control group (%) wettable powder 17.21 108 1.03 115 control group 15.96 - 0.89 - [Example 6]

(Testing the growth-promoting effect of seed treatment on sugar beet) Sugar beet (variety: Papirika) seeds were treated with a 1:1000 diluted aqueous solution of the wettable powder produced according to Example 3, using 0.1 ml of the solution per gram of seeds. After treatment the seeds are air-dried. As a control, sugar beet seeds were treated with the same amount of water and the seeds were air-dried in the same manner. On the second day, sugar beet seeds were sown in plastic cups T-600 (Tomei Chemical Industrial Co., Ltd.) filled with soil.

The fresh weight of the aboveground parts of the sugar beets was measured 52 days after sowing. The dry weight of the main root part of the plant was also measured after placing this part in a dryer set to 120°C for 3 days. Calculate the weight ratio relative to the control group using Equation 1.

The test results are presented in Table 3 below and Figure 3 below. The control group had a fresh weight of 7.44 g/plant in the aboveground part and a dry weight of 0.42 g/plant in the main root part. The wettable powder treatment group had a fresh weight of 8.79 g/plant in the above-ground part and a dry weight of 0.48 g/plant in the main root part, showing that the fresh weight of the above-ground part and the dry weight of the main root part were both higher than those in the control group. The fresh weight of the part and the dry weight of the main root part. There were no crop diseases or insect pests in the wettable powder treatment group and the control group. The wettable powder of Bacillus sp. D747 strain showed a growth-promoting effect on sugar beet.

[table 3] treatment group Fresh weight of aboveground parts (g/plant) Ratio of aboveground fresh weight to control group (%) Dry weight of main root part (g/plant) Ratio of dry weight of main root part to control group (%) wettable powder 8.79 118 0.48 114 control group 7.44 - 0.42 - [Example 7]

(Testing the high temperature stress relief effect of irrigation treatment on sugar beet) Sugar beet (variety: ^Papirika ) was tied in one of the sugar beet paper pots (for a set of 1,400 ( (20 rows × 70 columns) special paper buckets tied together, each measuring 19 mm in diameter and 130 mm in length, and both top and bottom open) are grown to the four leaf stage. A 1:500 diluted aqueous solution of the wettable powder produced according to Example 3 was then applied to the base of the sugar beets as an irrigation treatment, using 0.71 ml of the solution per sugar beet plant. For comparison, the same amount of water was used for the irrigation treatment of sugar beets (control group). On the second day of the irrigation treatment, the plants were transplanted into plastic cups T-600 (Tomei Chemical Industrial Co., Ltd.) filled with soil. After transplantation, the plants were grown under monitoring for 40 days on a bench with an outdoor roof. For the next 20 days, the growth of plants in the high-temperature group was monitored in the greenhouse, while the growth of plants in the normal-temperature group was monitored on an outdoor bench with a roof. During the 20-day period, the average temperature in the high temperature group was 27.9°C, and the highest and lowest temperatures were 40.7°C and 16.9°C respectively. In the normal temperature group, the average temperature during the 20-day period was 22.7°C, and the highest and lowest temperatures were 35.9°C and 13.5°C respectively.

After 60 days from transplantation, the beet taproot portion is crushed and filtered through a double gauze. The sugar content of the extract was then measured using a digital sweetness meter. Separately, the dry weight of the taproot portion was obtained by measuring after collecting the residue filtered through the gauze and the extract and placing the residue in a dryer set to 120°C for 4 days. Calculate the weight ratio relative to the control group using Equation 1.

The test results are presented in Table 4 below and Figure 4 below. The control group in the normal temperature group had a dry weight of 5.12 g/plant, and the sugar content of the main root was 14.6%. The control group in the high temperature group had a dry weight of 4.14 g/plant, and the sugar content of the main root part was 13.3%, which showed that under high temperature conditions, the dry weight and sugar content of the main root part were significantly reduced. In the wettable powder treatment group, the normal temperature group had a dry weight of 5.36 g/plant and the sugar content of the main root part was 15.5%, while the high temperature group had a dry weight of 4.78 g/plant and the sugar content of the main root part was 14.8 %, are higher than those observed in the corresponding control group. The difference in the high temperature group is particularly significant. There were no crop diseases or insect infestations in any test group. Wettable powders of Bacillus sp. D747 strain showed a growth-promoting effect on sugar beet, especially under high temperature conditions. The results indicate that the wettable powder of the Bacillus sp. D747 strain is expected to confer resistance to high temperature stress.

[Table 4] temperature treatment group Dry weight of main root part (g/plant) Ratio of dry weight of main root part to control group (%) Sugar content in the main root (%) Normal temperature group wettable powder 5.36 105 15.5 control group 5.12 - 14.6 high temperature group wettable powder 4.78 115 14.8 control group 4.14 - 13.3 [Example 8]

(Testing the aquatic stress alleviation effect of irrigation treatment on sugar beets) Sugar beets (variety: ^Papirika ) were tied in one of the sugar beet paper pots (for a set of 1,400 ( 20 rows × 70 columns) special paper buckets tied together, each measuring 19 mm in diameter and 130 mm in length, and both top and bottom open) are grown to the four-leaf stage. A 1:500 diluted aqueous solution of the wettable powder produced according to Example 3 was then applied to the base of the sugar beets as an irrigation treatment, using 0.71 ml of the solution per sugar beet plant. For comparison, the same amount of water was used for the irrigation treatment of sugar beets (control group). On the second day of the irrigation treatment, the plants were transplanted into plastic cups T-600 (Tomei Chemical Industrial Co., Ltd.) filled with soil. On days 10 to 21 after transplantation, plants in the aquatic stress group were submerged until water reached the soil surface in a plastic cup placed in the container. At other intervals, the plants were irrigated and monitored by soaking the bottom of the plastic cups with water each time the soil surface dried. For irrigation and monitoring of plants in the normal watering group, soak the bottom of the plastic cup with water every time the soil surface dries during the entire period after transplantation.

On the 28th day after transplantation, the dry weight of the taproot part of the sugar beet was measured after placing this part in a dryer set to 120°C for 3 days. Calculate the weight ratio relative to the control group using Equation 1.

The test results are presented in Table 5 below and Figure 5 below. The control group in the normal watering group had a dry weight of 0.70 g/plant in the main root part. The control group in the aquatic stress group had a dry weight of 0.45 g/plant in the main root part, which showed that the dry weight of the main root part was significantly reduced under aquatic stress conditions. In the wettable powder treatment group, the normal watering group had a dry weight of 0.78 g/plant in the main root part, and the aquatic stress group had a dry weight of 0.52 g/plant in the main root part, both of which were higher than those observed in the corresponding control group. to the value. The differences among the aquatic stress groups were particularly striking. There were no crop diseases or insect infestations in any test group. A wettable powder of Bacillus sp. strain D747 exhibits a growth-promoting effect on sugar beet, especially under aquatic stress conditions. The results indicate that wettable powders of the Bacillus sp. D747 strain are expected to confer resistance to aquatic stress.

[table 5] irrigation treatment group Dry weight of main root part (g/plant) Ratio of dry weight of main root part to control group (%) Normal watering group wettable powder 0.78 111 control group 0.70 - aquatic stress group wettable powder 0.52 116 control group 0.45 - [Example 9]

(Testing the aquatic stress relief effect of irrigation treatment on cabbage) Cabbage (variety: Yuina) was grown to the two-leaf stage in a 128-hole cell tray filled with nursery soil (Yosaku ^® , manufactured by JCAM AGRI Co., Ltd) (two leaf stages). Then, a 1:500 diluted aqueous solution of the wettable powder produced according to Example 3 was applied to the base of the cabbage as an irrigation treatment, using 2.6 ml of the solution for each cabbage plant. For comparison, the same amount of water was used for the irrigation treatment of cabbage (control group). On the second day of the irrigation treatment, the plants were transplanted into plastic cups T-600 (Tomei Chemical Industrial Co., Ltd.) filled with soil. On days 7 to 15 after transplantation, plants in the aquatic stress group were submerged until water reached the soil surface in a plastic cup placed in the container. At other intervals, the plants were irrigated and monitored by soaking the bottom of the plastic cups with water each time the soil surface dried. For irrigation and monitoring of plants in the normal watering group, soak the bottom of the plastic cup with water every time the soil surface dries during the entire period after transplantation.

The fresh weight of the above-ground parts of the cabbage was measured 25 days after transplantation. The dry weight of the underground part of the cabbage was also measured after placing it in a dryer that had been set to 120°C for 2 days. Calculate the weight ratio relative to the control group using Equation 1.

The test results are presented in Table 6 below and Figure 6 below. The control group under normal watering had a fresh weight of 12.81 g/plant in the above-ground part and a dry weight of 0.40 g/plant in the underground part. The control group of the aquatic stress group has a fresh weight of 10.84 g/plant in the above-ground part and a dry weight of 0.32 g/plant in the underground part. It shows that under aquatic stress conditions, the fresh weight of the above-ground part and the dry weight of the underground part are both equal. significantly reduced. In the wettable powder treatment group, the normal watering group had a fresh weight of 13.12 g/plant in the above-ground part and a dry weight of 0.44 g/plant in the underground part, while the aquatic stress group had a fresh weight of 12.11 g/plant in the above-ground part. fresh weight, and had a dry weight of 0.40 g/plant in the underground part, both higher than the values observed in the corresponding control group. The differences among the aquatic stress groups were particularly striking. There were no crop diseases or insect infestations in any test group. The wettable powder of Bacillus sp. D747 strain showed a growth-promoting effect on cabbage, especially under aquatic stress conditions. The results indicate that wettable powders of the Bacillus sp. D747 strain are expected to confer resistance to aquatic stress.

[Table 6] irrigation treatment group Fresh weight of aboveground parts (g/plant) Ratio of aboveground fresh weight to control group (%) Dry weight of underground parts (g/plant) Ratio of dry weight of underground part to control group (%) Normal watering group wettable powder 13.12 102 0.44 110 control group 12.81 - 0.40 - aquatic stress group wettable powder 12.11 112 0.40 125 control group 10.84 - 0.32 - [Example 10]

(Testing the growth-promoting effect of seed treatment on soybeans) Soybean (variety: Fukuyutaka) seeds were treated with a 1:100 diluted aqueous solution of the wettable powder produced according to Example 3, using 0.03 ml of the solution per gram of seeds. After treatment the seeds are air-dried. As a control, soybean seeds were treated with the same amount of water and air-dried in the same manner. On the second day, soybean seeds were sown in plastic cups T-600 (Tomei Chemical Industrial Co., Ltd.) filled with soil.

The fresh weight of the aboveground parts of the soybeans was measured 34 days after sowing. The dry weight of the underground part of the plant was also measured after placing this part in a dryer set to 120°C for 2 days. Calculate the weight ratio relative to the control group using Equation 1.

The test results are presented in Table 7 below and Figure 7 below. The control group had a fresh weight of 10.43 g/plant in the above-ground parts and a dry weight of 0.80 g/plant in the underground parts. The wettable powder treatment group had a fresh weight of 11.50 g/plant in the above-ground part and a dry weight of 0.86 g/plant in the underground part, showing that both the fresh weight of the above-ground part and the dry weight of the underground part were higher than those of the above-ground part in the control group. fresh weight and dry weight of underground parts. There were no crop diseases or insect pests in the wettable powder treatment group and the control group. The wettable powder of Bacillus genus D747 strain showed a growth-promoting effect on soybeans.

[Table 7] treatment group Fresh weight of aboveground parts (g/plant) Ratio of aboveground fresh weight to control group (%) Dry weight of underground parts (g/plant) Ratio of dry weight of underground part to control group (%) wettable powder 11.50 110 0.86 108 control group 10.43 - 0.80 - [Example 11]

(Testing the growth-promoting effect of irrigation treatment on green peppers) Green peppers (variety: Kyo-Hikari) were grown to the two-leaf stage in 128-hole cell trays filled with nursery soil (Yosaku ^® , manufactured by JCAM AGRI Co., Ltd). Then, a 1:500 diluted aqueous solution of the wettable powder produced according to Example 3 was applied to the base of the green peppers as an irrigation treatment, using 2.6 ml of the solution for each green pepper plant. For comparison, the same amount of water was used for the green pepper irrigation treatment (control group). On the second day of the irrigation treatment, the plants were transplanted into plastic cups T-600 (Tomei Chemical Industrial Co., Ltd.) filled with soil.

The fresh weight of the above-ground parts of the green peppers was measured 30 days after transplantation. The dry weight of the underground part of the green pepper was also measured after placing it in a dryer that had been set to 120°C for 2 days. Calculate the weight ratio relative to the control group using Equation 1.

The test results are presented in Table 8 below and Figure 8 below. The control group had a fresh weight of 2.11 g/plant in the above-ground parts and a dry weight of 0.21 g/plant in the underground parts. The wettable powder treatment group had a fresh weight of 2.40 g/plant in the above-ground part and a dry weight of 0.24 g/plant in the underground part, showing that both the fresh weight of the above-ground part and the dry weight of the underground part were higher than those of the above-ground part in the control group. fresh weight and dry weight of underground parts. There were no crop diseases or insect pests in the wettable powder treatment group and the control group. The wettable powder of Bacillus genus D747 strain showed growth-promoting effect on green pepper.

[Table 8] treatment group Fresh weight of aboveground parts (g/plant) Ratio of aboveground fresh weight to control group (%) Dry weight of underground parts (g/plant) Ratio of dry weight of underground part to control group (%) wettable powder 2.40 114 0.24 114 control group 2.11 - 0.21 - [Example 12]

(Testing the growth-promoting effect of hydroponic treatment on rapeseed) After moistening the GrowCube rock wool (10 mm × 10 mm × 7.5 mm) with sterilized distilled water, place it into the holes of the hole plate VIOLAMO ^® , and use germination to Rapeseed (variety: ALPAGA) seeds were sown 7 days later. The plates were then treated with a 1:100 diluted aqueous solution of the wettable powder produced according to Example 3, using 1 ml of the solution per well. After irrigation, add water as needed to prevent the hole from drying out.

The fresh weight of the aboveground parts of the rapeseed was measured 12 days after sowing.

The test results are presented in Table 9 below and Figure 9 below. The control group had a fresh weight of 0.062 g/plant in the above-ground parts. The wettable powder treatment group had a fresh weight of 0.095 g/plant in the above-ground parts, indicating that the fresh weight of the above-ground parts was higher than that in the control group. There were no crop diseases or insect pests in the wettable powder treatment group and the control group. The wettable powder of Bacillus sp. D747 strain showed a growth-promoting effect on rapeseed.

[Table 9] treatment group Fresh weight of aboveground parts (g/plant) Ratio of aboveground fresh weight to control group (%) wettable powder 0.095 153 control group 0.062 -

The invention can be summarized as follows.

The purpose of the present invention is to provide naturally derived, safe and available microbial materials that can help improve agricultural output by regulating crop growth and imparting resistance to environmental stress, while controlling plant diseases.

Among the solutions provided by the present invention are, for example, plant growth regulators and/or plant disease control agents, which contain live bacteria of specific strains of the genus Bacillus, or cultures containing such live bacteria as active ingredients. to regulate crop growth and impart resistance to environmental stress to increase yields.

The present inventors are based on the novel discovery that live Bacillus bacteria or cultures used for disease control have the effect of increasing yields by regulating crop growth and conferring resistance to environmental stress. The present invention is also based on the discovery of a method of using this effect. Reference Deposited Biological Materials

The registration numbers of deposited microorganisms relevant to the present invention are as follows. (1) Bacillus sp. D747 (FERM BP-8234).

[Figure 1] shows the growth-promoting effect on potatoes. [Figure 2] shows the growth-promoting effect of soil irrigation treatment on soybeans. [Figure 3] shows the growth-promoting effect on sugar beets. [Figure 4] shows the high-temperature stress relief effect on sugar beet. [Figure 5] shows the aquatic stress alleviating effect on sugar beet. [Figure 6] shows the aquatic stress relief effect on cabbage. [Figure 7] shows the growth-promoting effect of seed treatment on soybeans. [Figure 8] shows the growth-promoting effect on green pepper. [Fig. 9] shows the growth-promoting effect on rapeseed.

Claims (17)

A plant growth regulator is characterized by containing live bacteria or cultures of Bacillus subtilis or Bacillus amyloliquefaciens as active components, and has a plant growth promoting effect or a growth inhibiting effect. A plant growth regulator, characterized by comprising bacteria or cultures of one or more of live Bacillus sp. D747 strain, Bacillus subtilis HAI-0404 strain, Bacillus subtilis Y1336 strain, and Bacillus subtilis F727 strain As an active ingredient, it has plant growth promoting effect or growth inhibiting effect. A plant growth regulator is characterized in that it contains live bacteria or culture of Bacillus sp. D747 strain as an active component, and has a plant growth promoting effect or a growth inhibiting effect. The regulator as claimed in any one of claims 1 to 3, wherein in addition to the plant growth promotion effect or the growth inhibition effect, the regulator also confers resistance to environmental stress to the plant. The regulator of any one of claims 1 to 4, wherein the regulator has a plant disease control effect in addition to the plant growth promotion effect or the growth inhibition effect. The regulator of any one of claims 1 to 5, wherein the regulator regulates the growth of plants of the Amaranthaceae family (including Chenopodiaceae plants). The regulator of any one of claims 1 to 5, wherein the regulator regulates the growth of Solanaceae plants. The regulator of any one of claims 1 to 5, wherein the regulator regulates the growth of Fabaceae plants. The regulator of any one of claims 1 to 5, wherein the regulator regulates the growth of Brassicaceae plants. A method for regulating plant growth and/or conferring resistance to environmental stress and/or preventing plant diseases, characterized by comprising one or more plant growth regulators as claimed in any one of claims 1 to 9 contact with the plant and/or soil (especially the rhizosphere). The method of claim 10, wherein the plant is a plant seed. A seed processed by a method as claimed in claim 11. The method of claim 10, wherein the plant growth regulator is contacted with the soil through irrigation treatment or mixing into the soil treatment. A pharmaceutical agent for conferring resistance to environmental stress on plants, characterized in that it contains as active ingredient a living bacterium or culture of the genus Bacillus D747 strain. Such as the agent of claim 14, wherein the environmental pressure of the plant is high temperature pressure. Such as the agent of claim 14, wherein the plant environmental stress is aquatic stress. A method for conferring resistance to environmental stress on plants, characterized by the use of a medicament according to claim 14.

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