TW202322055A - Method of recognizing the drug residue in the food by the mass spectrum analysis and system of the same - Google Patents

Abstract

The present invention related to a method of recognizing the drug residue in the food by the mass spectrum analysis and system of the same. The system comprises an inspection device and an arithmetical device. The inspection device is used to examine the food sample to obtain inspection data. The arithmetical device is used to receive and save the inspection data. The arithmetical device also runs a data managing unit. The data managing unit is used to execute a comparison process about the inspection data according the predetermined parameter and the introduced maximum residue level to determine the food contain drug or not and the inspection data exceeds the limit or not.

Description

Method and system for identifying drug residues in food residues using mass spectrometry image analysis

The present invention relates to a method and system for identifying drug residues in food residues through mass spectrometry image analysis.

In order to make crops and animals grow healthily, relevant industry personnel can use drugs to achieve effects such as insect resistance, disease resistance, and growth promotion;

However, if there are too many drug residues in agricultural and livestock crops, it may harm the human body. Therefore, the competent government agency will set the allowable standard amount of residues, and cooperate with inspection and screening to prevent products that exceed the standard from entering the market;

However, when the relevant crops are pre-treated to make samples for inspection, various factors such as the ingredients of the crop itself may affect the inspection results, so that the contained drugs are not detected. In addition, some companies want to be more competitive in the market. , will also require their own products with stricter standards than the statutory standards. Therefore, how to strengthen the ability to interpret and identify drugs during inspections, and how to formulate customized inspection report standards for different clients are very important issues. However, there is currently no See that there are reliable systems that meet the aforementioned needs.

In view of the above shortcomings, the inventor believes that it is necessary to correct it. Based on his many years of experience in related technologies and product design and manufacturing, and adhering to the excellent design concept, he researched and created the above shortcomings. After continuous efforts, he finally achieved The method and system for identifying drug residues in food residues can be detected by using mass spectrometry image analysis of the present invention, hoping to correct the product structure and improve the product's excellent efficacy.

The main purpose of this invention is to provide a drug inspection management system architecture that can adjust the interpretation parameters to improve the drug identification ability and facilitate customized inspection standards. , and the method flow completed by using the system.

In order to achieve the purpose of the foregoing disclosure, the system for identifying drug residues in food residues in the present invention through mass spectrometry image analysis includes:

A testing device, used for testing drug residues on a sample to be tested, so as to obtain at least one piece of testing data, each piece of testing data has a plurality of data fields;

A computing device connected to the testing device, the computing device is used to receive and store various data including the testing data, and the computing device can be connected to an external database through a network;

A data management unit runs in the computing device, and the data management unit is used for displaying and allowing personnel to manage each inspection data.

The data management unit has a parameter setting subunit, an interface subunit, an import subunit, a data processing subunit, a warning subunit and an export subunit:

The parameter setting subunit is used for personnel to set various parameters when the inspection data is to be calculated and compared;

The interface subunit is used to import the legal allowable standard amount of drug residue from the external database;

The importing subunit is used for importing inspection data from the computing device into the data management unit;

The data processing subunit is used to perform calculation and comparison processing on the test data according to the comparison parameters and the allowable standard quantity, so as to determine whether there is any drug detected and whether each data field exceeds the predetermined limit value;

The warning subunit is used to mark the data fields exceeding the limit value with a predetermined color;

The exporting subunit is used to export the comparison result into a predetermined file type.

Therefore, when food residue drugs are detected by mass spectrometry image analysis, the steps to identify drug residues include: first, the inspection device inspects the sample to be inspected and transmits the data to the computing device for storage, and then the personnel use the data management unit to set parameters, Import the allowable standard amount of residue and import the data, and then the data management unit will calculate and compare the inspection data according to the comparison parameters and the allowable standard amount to determine whether there is any drug detected and whether each data field exceeds the predetermined limit value, the excess data field is marked with a predetermined color, and the final comparison result is exported to a predetermined file type.

And because the present invention can use the interface subunit to import the latest legal drug residue allowable standard amount from the external database, and use the parameter setting subunit to customize the comparison parameters, so in addition to depending on the crop type, the inspection can also be performed. Adjust the interpretation parameters according to various factors such as the brand of testing equipment used, the update of standard product data, or the testing experience of the testing personnel, so as to more accurately identify whether there are added drugs, and the parameter setting function can also achieve customizing for different clients. The purpose of customizing test report standards shows that the present invention has extremely high practical value in improving drug identification ability and controlling drug test data, and is a progressive creation.

[Please refer to Figure 1 and Figure 2] The present invention relates to a method and system for identifying drug residues in food residues through mass spectrometry image analysis. The system includes:

A testing device (1), used for testing drug residues on a sample to be tested to obtain at least one piece of testing data, each of which has multiple data fields;

A computing device (2), connected to the testing device (1), the computing device (2) is used to receive and store various data including the testing data, and the computing device (2) can be connected via a network to an external database (4);

A data management unit (3), running in the computing device (2), the data management unit (3) is used to display and allow personnel to manage the inspection data, the data management unit (3) has a parameter setting subunit (31), an interface subunit (32), an import subunit (33), a data processing subunit (34), a warning subunit (35) and an export subunit (36).

[Please refer to the first picture and the second picture] The inspection device (1) is used for qualitative and quantitative inspection of different components of a sample to be inspected;

After obtaining the inspection data of the sample, it is necessary to compare it with the inspection data of the drug standard to confirm whether each component is a certain drug. Therefore, before importing the data, first use the parameters of the data management unit (3) Set the sub-unit (31) to set various parameters during calculation and comparison;

The interface subunit (32) is used for importing legally permitted standard quantities of various drug residues from an external database (4) via the network;

After setting the parameters and importing the standard quantities of laws and regulations, the importing subunit (33) is used to import the test data in the computing device (2) into the data management unit (3), and then the data processing subunit (34 ) Comparing the test data with various comparison parameters and regulatory standard quantities to confirm whether each ingredient is a specific drug, whether the detected concentration exceeds the self-defined warning limit and regulatory standard quantity, etc., if it exceeds, then The warning subunit (35) marks the corresponding inspection data fields in different colors to achieve a warning effect, and finally the export subunit (36) can be used to export the comparison results into required file types.

[Please refer to Figures 1 to 5] For example, the testing device (1) is a liquid chromatography tandem mass spectrometer (LC-MS/MS) or a gas chromatography tandem mass spectrometer (GC-MS/ MS), etc., and after the sample is tested, each component test data stored in the computing device (2) will include columns such as residence time and relative ionic strength, and the computing device (2) is a computer or mobile phone, etc.;

Then, before importing the data for calculation and comparison, as shown in the third figure, use the parameter setting subunit (31) to set various comparison parameters, such as the allowable range of retention time, relative ion Intensity (Ion Ratio) allowable range, warning limit (Warning Limit), detection concentration reduction ratio of different types of agricultural products, etc., and use the interface subunit (32) to import various drug residue tolerances from external databases, such as the website of the competent authority. Standard quantity, where the competent authority is the Taiwan Food and Drug Administration (TFDA);

After the data is set and the interface is completed, as shown in the fourth and fifth figures, the import subunit (33) can be used to import the inspection data of the sample, and then the data processing subunit (34) will import the inspection data , Comparing the parameter and the allowable standard amount for calculation and comparison, if the residence time of a certain component falls within the allowable range of the residence time of a specific drug after comparison, it means that the drug has been detected (as shown in the fifth figure, it means The peak value of the chromatographic mass spectrum of this component falls within the allowable range of the peak value of the standard), and the column beyond the limit value will also be displayed in different colors by the warning subunit (35) to achieve the warning effect, as shown in the figure The relative ionic strength of the detected α-BHC (α-BHC) exceeds the relative ionic strength allowable range (Control Limit) of the regulations, so this column is marked in red, and the standard and sample of the warning can be checked in advance According to requirements, for example, three different color warnings can be used when only exceeding the self-defined warning limit, only exceeding the legal limit, and exceeding both the warning limit and the legal limit, so as to cooperate with the previous parameter setting function to provide different inspection commissioners Customized different inspection standards.

[Please refer to Figures 1 to 6] The above can be regarded as a method for identifying drug residues that can be detected by using mass spectrometry image analysis. The steps include:

An inspection program (S1), in which at least one piece of inspection data is obtained by inspecting a sample to be inspected by an inspection device (1), and each inspection data is then transmitted to and stored in a computing device (2);

A parameter setting program (S2), the operation device (2) runs a data management unit (3), and personnel use the parameter setting subunit (31) in the data management unit (3) to set comparison parameters;

An interface program (S3), the interface subunit (32) in the data management unit (3) imports the allowable standard quantity of residues of the drug to be tested from an external database (4) through the network;

an import program (S4), after the steps of the test program (S1) are completed, the import subunit (33) in the data management unit (3) imports the test data in the computing device (2) into the data In the management unit (3);

A data processing program (S5), the data processing subunit (34) in the data management unit (3) performs calculation and comparison processing on the inspection data according to the comparison parameters and the residual allowable standard amount, so as to confirm whether there is any detected Whether the drug and the predetermined data field exceed the limit value;

A warning program (S6), the warning subunit (35) in the data management unit (3) marks the data fields exceeding the limit value with a predetermined color;

An export program (S7), the comparison result is exported into a predetermined file type by the export subunit (36) in the data management unit (3).

[Please refer to the first figure, the second figure and the sixth figure] In addition, when performing the parameter setting procedure (S2) step through the parameter setting subunit (31), the following contents may also be included:

Set the drug groups whose detection concentrations must be combined and calculated. For example, the detection concentrations of α-BHC, β-BHC, γ-BHC, and δ-BHC must be combined and calculated and compared with the regulatory limit value of BHC. Therefore, in the parameter setting sub After the unit (31) has set the groups to be consolidated, the data processing sub-unit (34) will perform consolidated calculations in the data processing program (S5) and compare them with legal values;

Set the weighted ratio of multiple RT values to be collected for a single drug and the detection concentration of each RT value, [please also refer to the fifth figure] If you refer to the fifth figure, although some drugs will only be measured at a single RT value There are peaks, but some drugs will have peaks at multiple specific RT values. For example, Spinetoram may have two peaks (the RT values of Spinetoram J and Spinetoram L respectively), so the detection concentration of Spinetoram is calculated The method is to carry out a weighted sum of the detected concentrations of the two RT values, so after the parameter setting subunit (31) has set the weighted proportions of the multiple RT values to be collected and the detected concentrations of each RT value, it will The detection concentration of the drug is calculated by the data processing subunit (34) according to the set parameters.

What is described above is only a preferred embodiment of the present invention, and should not be used to limit the scope of the present invention. That is to say, all equivalent changes and modifications made according to the scope of the patent application should still fall within the scope covered by this creation patent.

To sum up, if the invention is novel and progressive, and the invention has not been published in any publication, it shall comply with the provisions of Article 22 of the Patent Law.

1: Inspection device 2: Computing device 3: Data management unit 31: Parameter setting subunit 32: Interface subunit 33: import subunit 34: Data processing subunit 35: Warning subunit 36: Export subunit 4: External database S1: Inspection procedure S2: Parameter setting procedure S3: Interface program S4: import program S5: Data processing program S6: Warning program S7: Export program

The first figure is a block diagram of the identification system for detecting drug residues in food residues of the present invention when using mass spectrometry image analysis. The second figure is a schematic diagram of the data management unit in the system for identifying drug residues that can detect drug residues through mass spectrometry image analysis of the present invention. The third figure is an example diagram of the parameter setting subunit of the identification system for detecting drug residues in food residues of the present invention when using mass spectrometry image analysis. Figure 4 is an example diagram of the data management unit of the identification system for detecting drug residues in food residues of the present invention when using mass spectrometry image analysis. The fifth figure is a schematic diagram of a chromatographic mass spectrum of a drug standard and its allowable range of retention time. Figure 6 is a flow chart of the steps of the method for identifying drug residues that can be detected by mass spectrometry image analysis of food residues of the present invention.

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1: Inspection device

2: computing device

3: Data management unit

4: External database

Claims (5)

A system for identifying drug residues in food residues through mass spectrometry image analysis, including: A testing device, used for testing drug residues on a sample to be tested, so as to obtain at least one piece of testing data, each piece of testing data has a plurality of data fields; A computing device connected to the inspection device, the computing device is used to receive and store the inspection data, and the computing device can be connected to an external database through the network; A data management unit, running in the computing device, the data management unit is used to display and allow personnel to manage the inspection data, the data management unit has a parameter setting subunit, an interface subunit, an import subunit, A data processing subunit, a warning subunit and an export subunit. The parameter setting subunit is used for personnel to set the comparison parameters when the inspection data is to be compared and processed. The interface subunit is used for Import the legal allowable standard amount of drug residue from the external database, the import subunit is used to import the test data from the computing device into the data management unit, and the data processing subunit is used to compare parameters and allowable The standard amount is used to carry out calculation and comparison processing on the test data to determine whether there is drug detection and whether each data field exceeds a predetermined limit value. The warning subunit is used to mark the data field that exceeds the limit value as Predetermined color, the export subunit is used to export the comparison result into a predetermined file type. The method and system for identifying drug residues in food residues when using mass spectrometry image analysis as described in claim 1, wherein the inspection device is liquid chromatography tandem mass spectrometer (LC-MS/MS) or gas chromatography Analytical tandem mass spectrometer (GC-MS/MS). A method for identifying residues of food residues that can be detected by mass spectrometry image analysis, the steps of which include: An inspection program, at least one piece of inspection data is obtained by inspecting the sample to be inspected by an inspection device, and each inspection data is then transmitted to and stored in a computing device, and each inspection data has a plurality of data fields; A parameter setting program, a data management unit is operated by the computing device, and personnel use the parameter setting subunit in the data management unit to set comparison parameters; An interface program, the interface subunit in the data management unit imports the allowable standard quantity of residues of the drug to be tested from an external database through the network; an import program, after the steps of the inspection program are completed, the import subunit in the data management unit imports the inspection data in the computing device into the data management unit; A data processing program, the data processing sub-unit in the data management unit performs calculation and comparison processing on the inspection data according to the comparison parameters and the residual allowable standard amount, so as to confirm whether there is any detected drug and whether the predetermined data field is has exceeded the limit value; A warning program, the warning sub-unit in the data management unit marks the data field exceeding the limit value with a predetermined color; An export program, the comparison result is exported into a predetermined file type by the export subunit in the data management unit. As described in claim 3, the method for identifying drug residues that can be detected by mass spectrometry image analysis of food residue drugs, wherein, in the parameter setting program step, it also includes setting the drug groups whose detection concentrations must be combined and calculated, and in the In the data processing program step, the combination calculation is performed and compared with the threshold value. As described in claim 3, the method for identifying drug residues that can be detected by using mass spectrometry image analysis for food residue drugs, wherein, in the parameter setting program step, it also includes setting a plurality of RT values and each RT value to be collected for a specific drug The weighted ratio of the detection concentration of the drug, and the detection concentration of the drug is calculated according to the set parameters in the data processing program step.

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