TW202245818A - Taiwan quinoa extract combination for lowering nonalcoholic fatty liver and use thereof - Google Patents

Abstract

A use of Taiwan quinoa extract combination includes: a Taiwan quinoa extract containing betanin and rutin which can be performed as an anti-oxidation ingredient and an ingredient for lowering nonalcoholic fatty liver. The Taiwan quinoa extract is a non-cytotoxic material.

Description

Composition of Taiwan quinoa extract that can reduce non-alcoholic fatty liver and its use

The present invention relates to a Taiwan quinoa (Taiwan quinoa) extract composition [extract combination] that can reduce nonalcoholic fatty liver [nonalcoholic fatty liver] and its use [use]; especially about a kind that can reduce nonalcoholic fatty liver Liver's compound Taiwan quinoa extract composition and its use.

Regarding the commonly used Taiwan quinoa extraction operation and its extract related technologies, for example: the invention patent of the Republic of China Patent Announcement No. TW-I426129 "A Method of Extracting Taiwan Quinoa to Increase the Production of Superoxide Dismutase", which discloses a way to increase superoxide dismutase Yield of Taiwan quinoa extraction method. The Taiwan quinoa extract method for improving the production of superoxide dismutase mainly includes an enzyme extraction step and a separation step.

Continuing from the above, the enzyme extraction step of the aforementioned patent announcement No. TW-I426129: provide a plant polysaccharide enzyme and a Taiwan quinoa powder, and use the plant polysaccharide enzyme to enzymatically hydrolyze the Taiwan quinoa powder to obtain an enzyme treatment solution.

Continuing from the above, the separation step of the aforementioned patent announcement No. TW-I426129: separate the enzyme treatment solution by centrifugation or filtration, and separate and obtain a precipitate that cannot be hydrolyzed by the enzyme to obtain a superoxide-containing Taiwan quinoa enzyme solution of dismutase.

Continuing from the above, the aforementioned patent announcement No. TW-I426129 can choose to operate a pre-step before the enzyme extraction step: extracting a piece of Taiwan quinoa Drying the material to obtain a dried Chenopodium material, and grinding the dried Chenopodium material to obtain the Chenopodium powder.

However, the method for extracting the Chenopodium in the aforementioned patent announcement No. TW-I426129 only discloses the processing of the Chenopodium material in the pre-steps, enzyme extraction steps and separation steps to obtain the Chenopodium ferment solution containing superoxide dismutase, And it is only simply used to provide the Taiwan quinoa ferment liquid containing superoxide dismutase.

Another commonly used Taiwan quinoa extraction operation and its extract related technology, for example: Republic of China Patent Announcement No. TW-I426915 "Use of Taiwan quinoa for the preparation of medicines for treating hyperlipidemia and hypercholesterolemia and its pharmaceutical composition" Invention patent, which discloses the use of Taiwan quinoa for the preparation of medicines for treating hyperlipidemia and hypercholesterolemia. The use of the drug uses Taiwan quinoa pulp as an active ingredient.

Continuing from the above, the method for preparing a drug for treating hyperlipidemia and hypercholesterolemia in the aforementioned patent announcement No. TW-I426915 includes: preparing a sample of Taiwan quinoa, grinding the sample to a particle size of 1-20 μm, and mixing it with distilled water, to obtain a micron-sized mixture.

Continuing from the above, the method for preparing a drug for treating hyperlipidemia and hypercholesterolemia in the aforementioned patent announcement No. TW-I426915 includes: then, grinding the micron-scale mixture to a particle size of 20-70nm to obtain a nano-scale mixture ; Next, heat the nanoscale mixture at 65-85° C. for 0.5-1 hour.

Continuing from the above, the method for preparing a drug for treating hyperlipidemia and hypercholesterolemia in the aforementioned patent announcement No. TW-I426915 includes: then, at 37°C, decompose the heated nanoscale mixture with an amylase for 4-8 hours to obtain an enzymatic decomposition liquid; then, concentrating the enzymatic decomposition liquid to obtain a Taiwan quinoa pulp.

Yet the above-mentioned patent announcement No. TW-I426915 that this Taiwan quinoa is used for preparing the medicine for treating hyperlipidemia and hypercholesterolemia only discloses adopting The Taiwan quinoa material is decomposed and treated with the amylase to obtain the enzymatic decomposition liquid, and the enzymatic decomposition liquid is further concentrated to obtain the Taiwan quinoa pulp, and the Taiwan quinoa pulp is only used to prepare and treat hyperlipidemia and hyperlipidemia. Cholesterol medicine.

Another commonly used Taiwan quinoa extract and related technologies, for example: the invention patent application of the Republic of China Patent Publication No. TW-201912177 "Taiwan quinoa shell extract with whitening and anti-aging functions and its extraction method", It discloses a Taiwan quinoa shell extraction method. The extraction method of the Taiwan quinoa shell comprises: selecting a Taiwan quinoa shell, sequentially performing alcohol extraction, filtering and drying the Taiwan quinoa shell to obtain a preliminary extract.

As mentioned above, the preliminary extract of the aforementioned patent publication No. TW-201912177 can be further purified or differentiated, so that the components of different properties of the preliminary extract can be separated or directly used in a product, and a purified product can be obtained after purification. A purified composition, and the purified composition can be used for anti-oxidation, whitening or anti-aging skin applications.

However, the Taiwan quinoa shell extraction method of the aforementioned patent publication No. TW-201912177 only discloses that the Taiwan quinoa shell is processed by alcohol extraction, filtration and drying to obtain the preliminary extract or purified composition of the Taiwan quinoa shell, and the This purified composition is only used purely for anti-oxidation, whitening or anti-aging skin applications.

Another commonly used Taiwan quinoa extraction operation and its extract related technologies, for example: Republic of China Patent Publication No. TW-202103680 "The use of Taiwan quinoa extract for preparing compositions that resist natural aging, promote fat decomposition and reduce fat accumulation and "Fat-reducing Composition" patent application for invention, which discloses a Taiwan quinoa extract. The extract of Chenopodium triosporum is heated and extracted with a solvent containing water to obtain a primary extract, and the primary extract is fermented with saccharification enzymes to obtain a fermented extract of Chenopodium triosporum.

Continuing from the above, the aforementioned patent publication No. TW-202103680 Taiwan quinoa extract contains: guanosine [Guanosine], trans-3-indoleacrylic acid [trans-3-Indoleacrylic acid], 4-glucosylvanillic acid [4-Glucosylvanillic acid], icariside B1 [Icariside B1], protocatechuic acid [Protocatechuic acid], gentisic acid [Gentisic acid], caffeic acid [Caffeic acid], vanillic acid [Vanillic acid], rutin [Rutin], quercetin-3-rutinoside- 7-rhamnoside [Quercetin-3-O-rutinoside-7-O-rhamnoside], coumaric acid [Coumaric acid], ferulic acid [Ferulic acid], 20-hydroxyecdysone [20-Hydroxyecdysone], quercetin Glycoside [Quercetrin], Astragalin [Astragalin], Kaempferol-3-rhamnoside [kaempferol-3-rhamnoside], Kaempferol-7-rhamnoside [Kaempferol-7-rhamnoside], Quercetin [Quercetin], Kaempferol [Kaempferol], Oleanolic acid [Oleanolic acid], Albinoside B [Vincetoxicoside B].

However, the aforementioned patent publication No. TW-202103680 of the Taiwan quinoa fermented extract only discloses the use of aqueous solvents for extraction treatment and glycolysis enzymes for fermentation treatment to obtain the Taiwan quinoa extract, and the Taiwan quinoa The fermented extract is only used to prepare compositions for resisting natural aging, promoting fat decomposition and reducing fat accumulation.

Another commonly used Taiwan quinoa seed extraction operation and its extract related technologies, for example: the invention patent application of the Republic of China Patent Publication No. TW-201817434 "Method for Extracting Antioxidant Components from Taiwan Quinoa Seeds", which discloses a Taiwan quinoa seed A method of extracting antioxidant components from The method for extracting antioxidant components from the seeds of the Taiwan quinoa comprises: crushing the seeds of the Taiwan quinoa to a particle size of about 0.15 to 1 mm, so as to obtain a crushed grain of the seeds of the Taiwan quinoa.

Continuing from the above, the method for extracting antioxidant components from the Taiwan quinoa seeds in the aforementioned patent publication No. TW-201817434 further includes: mixing and shaking the Taiwan quinoa seeds with an aqueous ethanol solution to obtain a Taiwan quinoa species sub-extraction.

Continuing from the above, the method for extracting antioxidant components from the Taiwan quinoa seed in the aforementioned patent publication No. TW-201817434 further includes: after separating the Taiwan quinoa seed extract, then centrifugally separating the Taiwan quinoa seed extract extracts and solvents in order to obtain a quinoa seed extract.

However, the aforesaid patent publication No. TW-201817434 for the extract of the quinoa seeds only discloses that the quinoa seeds are extracted with an aqueous ethanol solution to obtain the extract of the quinoa seeds. Obviously, the extract of the quinoa seeds does not Does not belong to the extracts of other parts (non-seeds) of Taiwan quinoa.

Another commonly used Taiwan quinoa shell processing operation and its composition related technologies, for example: the invention patent application of the Republic of China Patent Publication No. TW-201828965 "Use of Taiwan quinoa composition for the preparation of blood sugar-lowering drugs", which discloses a The composition of Taiwan quinoa is used for the preparation of a drug for lowering blood sugar. The Taiwan quinoa composition is administered to the individual in need before meals to suppress the rise in blood sugar.

Continuing from the above, the Taiwan quinoa composition of the aforementioned patent publication No. TW-201828965 is a mixture of grain flour of the shell of Taiwan quinoa and water, and the grain flour of the Taiwan quinoa composition contains a dietary fiber, and the dietary fiber accounts for the total weight of the grain flour 60% to 80% of that.

However, the aforesaid patent publication No. TW-201828965 discloses the composition of the quinoa husk by mixing the grain flour and water with the husk of the chenopodium to obtain a composition of the husk of the husk, and the composition of the husk of the husk is simply It is used to lower blood sugar. Obviously, the shell composition of Taiwan quinoa does not belong to the extract of Taiwan quinoa.

Obviously, the aforementioned Republic of China Patent Announcement No. TW-I426129 for Taiwan Chenopodium extract, No. TW-I426915 for Taiwan Chenopodium extract, Republic of China Patent Publication No. TW-201912177 for Taiwan Chenopodium extract, The Taiwan quinoa extract of TW-202103680, the Taiwan quinoa seed extract of TW-201817434, and the Taiwan quinoa shell composition of TW-201828965 are not suitable for reducing non-alcoholic fatty liver, so there are still further Provide the needs for reducing non-alcoholic fatty liver function.

In short, the inventions of the aforementioned ROC Patent Publication No. TW-I426129, TW-I426915, ROC Patent Publication No. TW-201912177, TW-202103680, TW-201817434 and TW-201828965 The disclosures of patents and patent applications are only for reference of the technical background of the present invention and to illustrate the current state of technological development, and are not intended to limit the scope of the present invention.

In view of this, in order to meet the above-mentioned technical problems and needs, the present invention provides a composition of Taiwan quinoa extract that can reduce non-alcoholic fatty liver and its use, wherein the Taiwan quinoa extract contains a betaine component and a rutin component, and the Taiwan quinoa extract can be used as an anti-oxidation [DPPH free radical scavenging, hydroxyl scavenging and lipid oxidation inhibition] and the application of reducing non-alcoholic fatty liver, and the Taiwan quinoa extract does not have cell Toxicity, in order to increase the application range and economic value of Taiwan quinoa extract.

The main purpose of the present invention is to provide a Taiwan quinoa extract composition and its application that can reduce non-alcoholic fatty liver, wherein a Taiwan quinoa extract contains a betaine component and a rutin component, and the Taiwan quinoa Wheat extract can be used as anti-oxidation [DPPH free radical scavenging, hydroxyl scavenging and lipid oxidation inhibition] and application of reducing non-alcoholic fatty liver, and the Taiwan quinoa extract is not cytotoxic, so as to achieve the improvement of Taiwan quinoa The purpose and effect of economic value.

In order to achieve the above purpose, the use of Taiwan quinoa extract that can reduce non-alcoholic fatty liver according to a preferred embodiment of the present invention includes:

A Taiwan quinoa extract is extracted from a Taiwan quinoa material, and the Taiwan quinoa extract contains a betaine component and a rutin component, and The Taiwan quinoa extract can be used as an anti-oxidation [DPPH free radical scavenging, hydroxyl scavenging and lipid oxidation inhibition] and application for reducing non-alcoholic fatty liver, and the Taiwan quinoa extract has no cytotoxicity.

A black quinoa extract, a red quinoa extract or a white quinoa extract are added to the Taiwan quinoa extract in a preferred embodiment of the present invention to form a compound Taiwan quinoa extract.

The Taiwan quinoa extract in a preferred embodiment of the present invention is extracted from a Taiwan quinoa body, a Taiwan quinoa fragment, a Taiwan quinoa powder or any combination thereof.

In order to achieve the above purpose, the Taiwanese quinoa extract composition capable of reducing non-alcoholic fatty liver according to a preferred embodiment of the present invention includes:

at least one food material having a first content; and

At least one Taiwan quinoa extract, which has a second content, and the Taiwan quinoa extract is extracted from a Taiwan quinoa material, and the Taiwan quinoa extract is added to the food material to prepare a food composition;

Among them, the Taiwan quinoa extract contains a betaine component and a rutin component, and the Taiwan quinoa extract can act as an antioxidant [DPPH free radical scavenger, hydroxyl scavenger and lipid oxidation inhibition] and reduce non-alcoholic fat Liver application, and the Taiwan quinoa extract is not cytotoxic.

A black quinoa extract, a red quinoa extract or a white quinoa extract are added to the Taiwan quinoa extract in a preferred embodiment of the present invention to form a compound Taiwan quinoa extract.

The Taiwan quinoa extract in a preferred embodiment of the present invention is extracted from a Taiwan quinoa body, a Taiwan quinoa fragment, a Taiwan quinoa powder or any combination thereof.

The food composition in a preferred embodiment of the present invention is a drink, a food, a seasoning sauce, a seasoning powder, an edible oil or a dairy product.

In order to achieve the above object, the preferred embodiment of the present invention can reduce The composition of Taiwan quinoa extract with low non-alcoholic fatty liver disease contains:

at least one health food ingredient having a first content; and

At least one Taiwan quinoa extract has a second content, and the Taiwan quinoa extract is extracted from a Taiwan quinoa material, and the Taiwan quinoa extract is added to the health food raw material to prepare a Healthy food composition;

Among them, the Taiwan quinoa extract contains a betaine component and a rutin component, and the Taiwan quinoa extract can act as an antioxidant [DPPH free radical scavenger, hydroxyl scavenger and lipid oxidation inhibition] and reduce non-alcoholic fat Liver application, and the Taiwan quinoa extract is not cytotoxic.

A black quinoa extract, a red quinoa extract or a white quinoa extract are added to the Taiwan quinoa extract in a preferred embodiment of the present invention to form a compound Taiwan quinoa extract.

The Taiwan quinoa extract in a preferred embodiment of the present invention is extracted from a Taiwan quinoa body, a Taiwan quinoa fragment, a Taiwan quinoa powder or any combination thereof.

The health food raw material in a preferred embodiment of the present invention is selected from a nutritional supplement raw material, a dietary supplement raw material or a health food raw material, so as to make a nutritional supplement, a dietary supplement, a health food or a health food .

The health food raw material in a preferred embodiment of the present invention is selected from a powdery raw material, a pasty raw material or a liquid raw material.

1a: Food ingredients

1b: Health food ingredients

100: Processing equipment

2: Taiwan quinoa extract

20: Compound Taiwan Quinoa Extract

2a: Black quinoa extract

2b: Red quinoa extract

2c: White quinoa extract

3a: Food composition

3b: Health food composition

Figure 1: A schematic block diagram of Taiwanese quinoa-added products made from the Taiwanese quinoa extract composition that can reduce non-alcoholic fatty liver according to the first preferred embodiment of the present invention.

Figure 2: The second preferred embodiment of the present invention can reduce non-alcoholic fatty liver of Taiwan quinoa extract composition made of Taiwan quinoa additive products Block diagram.

Figure 3: A schematic diagram of the Taiwanese quinoa extract composition that can reduce non-alcoholic fatty liver in the third preferred embodiment of the present invention using a compound Taiwanese quinoa extract.

Figure 4: A schematic diagram showing the ability of various wheat extracts to scavenge DPPH free radicals of the Taiwan quinoa extract composition that can reduce non-alcoholic fatty liver in a preferred embodiment of the present invention.

Figure 5A: a schematic diagram of the Taiwan quinoa extract composition of the preferred embodiment of the present invention that can reduce non-alcoholic fatty liver. The Taiwan quinoa extract does not have cytotoxicity.

Fig. 5B: A schematic diagram of the Taiwanese quinoa extract composition capable of reducing non-alcoholic fatty liver according to a preferred embodiment of the present invention, in which the black quinoa extract does not have cytotoxicity.

Figure 5C: a schematic diagram of the red quinoa extract of the Taiwanese quinoa extract composition that can reduce non-alcoholic fatty liver in a preferred embodiment of the present invention without cytotoxicity.

Figure 5D: a schematic diagram of the Taiwanese quinoa extract composition capable of reducing non-alcoholic fatty liver according to a preferred embodiment of the present invention, in which the quinoa extract does not have cytotoxicity.

Figure 6A: a schematic diagram of the ability of Taiwan quinoa extract to reduce non-alcoholic fatty liver in the composition of Taiwan quinoa extract that can reduce non-alcoholic fatty liver according to a preferred embodiment of the present invention.

Figure 6B: a schematic diagram of the ability of the quinoa extract composition in Taiwan that can reduce non-alcoholic fatty liver according to a preferred embodiment of the present invention to reduce non-alcoholic fatty liver using black quinoa extract.

Figure 6C: a schematic diagram of the ability of the Taiwan quinoa extract composition that can reduce non-alcoholic fatty liver by using red quinoa extract in a preferred embodiment of the present invention to reduce non-alcoholic fatty liver.

Figure 6D: The preferred embodiment of the present invention can reduce non-alcoholic fatty liver Schematic diagram of the ability of the Taiwan quinoa extract composition to reduce non-alcoholic fatty liver with the use of quinoa extract.

In order to fully understand the present invention, preferred embodiments will be described below in detail together with the accompanying drawings, which are not intended to limit the present invention.

The Taiwanese quinoa extract composition and its application that can reduce non-alcoholic fatty liver in a preferred embodiment of the present invention can be applied to quinoa pieces of various quinoa [black quinoa, red quinoa, white quinoa or other varieties of quinoa] body, pieces, powder or other solids, and the composition of Taiwan quinoa extract that can reduce non-alcoholic fatty liver and its use according to the preferred embodiment of the present invention can be combined and applied in various automatic equipment and various semi-automatic equipment or various non-automatic equipment, but it is not intended to limit the scope of application of the present invention.

Figure 1 shows a schematic block diagram of Taiwan quinoa-added products made from the Taiwanese quinoa extract composition that can reduce non-alcoholic fatty liver according to the first preferred embodiment of the present invention. Please refer to Figure 1, for example, the Taiwanese quinoa extract composition that can reduce non-alcoholic fatty liver in the first preferred embodiment of the present invention can be appropriately selected to make a Taiwan quinoa added product, and the The Taiwan quinoa additive product comprises at least one food material 1a and at least one Taiwan quinoa extract 2, and the food material 1a and the Taiwan quinoa extract 2 are made into a food composition 3a.

Please refer to Figure 1 again, for example, the Taiwan quinoa extract 2 can be selected from a Taiwan quinoa alcohol extract, and the Taiwan quinoa extract 2 contains a betanin [Betanin] component and a rue Glycoside [Rutin] component. The Taiwan quinoa extract 2 also contains amaranthine [Amaranthine], Kaempferol-3,7-di- O -rhamnoside, Kaempferol-3- O -robinobioside and Kaempferol-3- O -rutinoside.

Please refer to Figure 1 again, for example, using a processing equipment 100 [or other equipment with similar functions] to add the Taiwan quinoa extract 2 to the food material 1a by appropriate technical means, and the Taiwan quinoa wheat The extract 2 can be extracted from a body of Taiwan quinoa, a piece of Taiwan quinoa (as shown on the right side of Figure 1), a powder of Taiwan quinoa (as shown on the right side of Figure 1) or any combination thereof.

Please refer to Fig. 1 again, for example, the food composition 3a can be selected as a drink, a food, a seasoning sauce, a seasoning powder, an edible oil product, a dairy product, other food products [for example: Various snack products, various bread products or various pastry products] or other food processing products [for example: various meat products].

Figure 2 shows a schematic block diagram of Taiwan quinoa-added products made from the Taiwanese quinoa extract composition that can reduce non-alcoholic fatty liver according to the second preferred embodiment of the present invention. Please refer to Figure 2, compared to the first embodiment, the Taiwan quinoa added product of the second preferred embodiment of the present invention includes at least one health food raw material 1b and at least one Taiwan quinoa extract 2, and the The health food raw material 1a and the Taiwan quinoa extract 2 are made into a health food composition 3b.

Please refer to Fig. 2 again, for example, the health food raw material 1b can be selected from a nutritional supplement raw material, a dietary supplement raw material or a health food raw material, so as to make a nutritional supplement, a dietary supplement , a health food, a health food or other products.

Please refer to Figure 1 again, for example, the health food raw material 1b can be selected from a powder raw material [power material], a paste raw material [paste material], a liquid raw material [liquid material] or any combination thereof raw materials, and the powder raw materials, paste raw materials or liquid raw materials can be selected according to different needs.

Fig. 3 shows a schematic diagram of the Taiwan quinoa extract composition that can reduce non-alcoholic fatty liver according to the third preferred embodiment of the present invention using compound Taiwan quinoa extract. Please refer to FIG. 3 , compared to the first and second embodiments, the Taiwanese quinoa-added product of the third preferred embodiment of the present invention uses at least one compound Taiwan quinoa extract 20 .

Please refer to Figure 3 again. For example, the compound Taiwan quinoa extract 20 of the Taiwan quinoa-added product can be selected to add at least one black quinoa (alcohol) extraction to the Taiwan quinoa extract 2 2a, at least one red quinoa (alcohol) extract 2b, at least one white quinoa (alcohol) extract 2c or other similar extracts, so as to form the compound Taiwan quinoa extract 20 or other related Taiwan quinoa extract combination thing.

Please refer to Figure 3 again. For example, the black quinoa extract 2a, the red quinoa extract 2b and the white quinoa extract 2c are respectively extracted from at least one black quinoa material [or a material containing black quinoa components ], at least one red quinoa material [or a material containing a red quinoa component] and at least one white quinoa material [or a material containing a white quinoa component].

Figure 4 shows a schematic diagram showing the ability of various wheat extracts to scavenge DPPH free radicals of the Taiwan quinoa extract composition that can reduce non-alcoholic fatty liver according to a preferred embodiment of the present invention. Please refer to Figure 4, for example, the Taiwanese quinoa extract 2, black quinoa extract 2a, red quinoa extract 2b and white quinoa extract 2c all have the ability to scavenge DPPH free radicals (that is, antioxidant activity) , and the Taiwan quinoa extract 2, black quinoa extract 2a, red quinoa extract 2b and white quinoa extract 2c all have a concentration-dependent ability to scavenge DPPH free radicals, so they can choose to use different concentrations according to different needs .

FIG. 5A shows a schematic diagram of the Taiwanese quinoa extract composition capable of reducing non-alcoholic fatty liver according to a preferred embodiment of the present invention, in which the Taiwanese quinoa extract does not have cytotoxicity. Please refer to Figure 5A, for example, when the Taiwan quinoa extract composition of the Taiwan quinoa extract composition that can reduce non-alcoholic fatty liver in a preferred embodiment of the present invention is selected and implemented on the human liver cancer cell line Huh7, The cell survival rate reaches 100% at the concentration of 0-250μg/mL, and the cell survival rate still reaches 89% when the concentration is increased to 500μg/mL.

Figure 5B reveals that the black quinoa extract of the Taiwan quinoa extract composition that can reduce non-alcoholic fatty liver according to the preferred embodiment of the present invention does not have Schematic representation of cytotoxicity. Please refer to Figure 5B, for example, when the black quinoa extract of the Taiwanese quinoa extract composition that can reduce non-alcoholic fatty liver in a preferred embodiment of the present invention is selected and implemented on the human liver cancer cell line Huh7, in The cell viability was maintained at 100% at a concentration of 0-500μg/mL.

Fig. 5C shows a schematic diagram of the red quinoa extract of the Taiwanese quinoa extract composition capable of reducing non-alcoholic fatty liver according to a preferred embodiment of the present invention without cytotoxicity. Please refer to Figure 5C, for example, when the red quinoa extract of the Taiwanese quinoa extract composition that can reduce non-alcoholic fatty liver in a preferred embodiment of the present invention is selected and implemented on the human liver cancer cell line Huh7, the The cell viability was maintained at 100% at a concentration of 0-500μg/mL.

FIG. 5D shows a schematic diagram of the Taiwanese quinoa extract composition capable of reducing non-alcoholic fatty liver according to a preferred embodiment of the present invention, and the quinoa extract does not have cytotoxicity. Please refer to Figure 5D, for example, when the Taiwan quinoa extract composition of the Taiwan quinoa extract composition that can reduce non-alcoholic fatty liver in a preferred embodiment of the present invention is selected and implemented on the human liver cancer cell line Huh7, The cell survival rate reaches 100% at the concentration of 0-125μg/mL, and the cell survival rate will still reach 95% when the concentration is increased to 250μg/mL, and the cell survival rate will still reach 89.8% when the concentration is increased to 500μg/mL .

Figure 6A shows a schematic diagram of the ability of Taiwan quinoa extract to reduce non-alcoholic fatty liver in the composition of Taiwanese quinoa extract that can reduce non-alcoholic fatty liver according to a preferred embodiment of the present invention. Please refer to Figure 6A, for example, the Taiwan quinoa extract composition that can reduce non-alcoholic fatty liver in a preferred embodiment of the present invention uses Taiwan quinoa extract [10μg/mL, 25μg/mL, 50μg /mL, 75μg/mL, 100μg/mL] and oleic acid [Oleic acid] induced selection were added to the human liver cancer cell line Huh7, compared with no addition of Taiwan quinoa extract, they all reduced fat accumulation in cells, and their Not concentration dependent.

Figure 6B reveals the Taiwan quinoa extract composition that can reduce non-alcoholic fatty liver according to a preferred embodiment of the present invention. Schematic representation of low NAFLD capacity. Please refer to Figure 6B, for example, the Taiwan quinoa extract composition that can reduce non-alcoholic fatty liver mL, 75μg/mL, 100μg/mL] and oleic acid were selected and added to the human liver cancer cell line Huh7, compared with no addition of black quinoa extract, it showed that they all reduced fat accumulation in cells, at 10μg/mL, 25μg/mL, 50μg/mL has a good ability to reduce fat accumulation in cells and has no concentration dependence, and the ability to reduce fat accumulation in cells is second best at 75μg/mL and 100μg/mL.

Fig. 6C shows a schematic diagram of the ability of the red quinoa extract to reduce non-alcoholic fatty liver in the Taiwan quinoa extract composition that can reduce non-alcoholic fatty liver according to a preferred embodiment of the present invention. Please refer to Figure 6C, for example, the Taiwan quinoa extract composition that can reduce non-alcoholic fatty liver mL, 75 μg/mL, 100 μg/mL] and oleic acid were added together to the human liver cancer cell line Huh7, compared with no addition of red quinoa extract, they all reduced fat accumulation in cells, and it was not concentration-dependent.

FIG. 6D shows a schematic diagram of the ability of the Taiwan quinoa extract composition that can reduce non-alcoholic fatty liver using the quinoa extract to reduce non-alcoholic fatty liver according to a preferred embodiment of the present invention. Please refer to Figure 6D, for example, the Taiwan quinoa extract composition that can reduce non-alcoholic fatty liver mL, 75μg/mL, 100μg/mL] and oleic acid were selected and added to the human liver cancer cell line Huh7, compared with no addition of resveratilla extract, they all reduced fat accumulation in cells. The ability to reduce fat accumulation in cells is good and has no concentration dependence, and the ability to reduce fat accumulation in cells is the second best at 10 μg/mL, 75 μg/mL, and 100 μg/mL.

In addition, the preferred embodiment of the present invention can reduce non-alcoholic fat The composition of Taiwan quinoa extract for fatty liver was analyzed by gas chromatography mass spectrometry. After the four kinds of quinoa extracts act on the cells, the values of myristic acid, palmitic acid and palmitoleic acid in the cells will decrease. It is speculated that the four kinds of quinoa All can relieve cell inflammation, oxidative stress and endoplasmic reticulum pressure, further reduce lipid accumulation, and slow down fatty liver.

The above experimental data are preliminary experimental results obtained under specific conditions, which are only used for easy understanding or reference to the technical content of the present invention, and other related experiments are still required. The experimental data and its results are not intended to limit the scope of the present invention.

The above-mentioned preferred embodiments only illustrate the present invention and its technical characteristics, and the technology of this embodiment can still be implemented in various substantially equivalent modifications and/or replacements; therefore, the scope of rights of the present invention depends on the appended patent application The scope defined by the scope shall prevail. The copyright in this case is restricted to be used for patent applications in the Republic of China.

1a: Food ingredients

100: Processing equipment

2: Taiwan quinoa extract

3a: Food composition

Claims (10)

A use of Taiwan quinoa extract that can reduce non-alcoholic fatty liver, which includes: A Taiwan quinoa extract is extracted from a Taiwan quinoa material, and the Taiwan quinoa extract contains a betaine component and a rutin component, and the Taiwan quinoa extract can act as an antioxidant [DPPH free radical scavenging, hydrogen Oxygen scavenging and inhibition of lipid oxidation] and the application of reducing non-alcoholic fatty liver, and the Taiwan quinoa extract is not cytotoxic. Use of Taiwanese quinoa extract that can reduce non-alcoholic fatty liver as described in item 1 of the patent application, wherein the Taiwanese quinoa extract is additionally added with a black quinoa extract, a red quinoa extract or a white quinoa extract Extract, in order to form a complex Taiwan quinoa extract. The use of Taiwan quinoa extract that can reduce non-alcoholic fatty liver as described in item 1 of the patent application, wherein the Taiwan quinoa extract is extracted from a Taiwan quinoa body, a Taiwan quinoa fragment, and a Taiwan quinoa Quinoa powder or any combination thereof. A Taiwanese quinoa extract composition capable of reducing non-alcoholic fatty liver, comprising: at least one food material having a first content; and At least one Taiwan quinoa extract, which has a second content, and the Taiwan quinoa extract is extracted from a Taiwan quinoa material, and the Taiwan quinoa extract is added to the food material to prepare a food composition; Wherein the Taiwan quinoa extract contains a betaine component and a rutin component, and the Taiwan quinoa extract can be used as an anti-oxidant and reduce non-alcoholic fatty liver, and the Taiwan quinoa extract is not cytotoxic . The Taiwanese quinoa extract composition capable of reducing non-alcoholic fatty liver described in item 4 of the patent application, wherein the food composition is a drink, a food, a seasoning sauce, a seasoning powder, and an edible oil or a dairy product. A Taiwanese quinoa extract composition capable of reducing non-alcoholic fatty liver, comprising: at least one health food ingredient having a first content; and At least one Taiwan quinoa extract has a second content, and the Taiwan quinoa extract is extracted from a Taiwan quinoa material, and the Taiwan quinoa extract is added to the health food raw material to prepare a Healthy food composition; Wherein the Taiwan quinoa extract contains a betaine component and a rutin component, and the Taiwan quinoa extract can be used as an anti-oxidant and reduce non-alcoholic fatty liver, and the Taiwan quinoa extract is not cytotoxic . The Taiwanese quinoa extract composition capable of reducing non-alcoholic fatty liver according to item 6 of the patent application, wherein the health food raw material is selected from a nutritional supplement raw material, a dietary supplement raw material or a health food raw material, To make a nutritional supplement, a dietary supplement, a health food or a health food. The Taiwanese quinoa extract composition capable of reducing non-alcoholic fatty liver according to item 6 of the patent application, wherein the health food raw material is selected from a powder raw material, a paste raw material or a liquid raw material. The Taiwanese quinoa extract composition that can reduce non-alcoholic fatty liver according to item 4 or 6 of the patent application, wherein the Taiwanese quinoa extract is additionally added with a black quinoa extract, a red quinoa extract or a White quinoa extract to form a compound Taiwan quinoa extract. The Taiwanese quinoa extract composition capable of reducing non-alcoholic fatty liver according to item 4 or 6 of the patent application, wherein the Taiwanese quinoa extract is extracted from a Taiwanese quinoa body, a Taiwanese quinoa fragment, 1 Taiwanese quinoa powder or any combination thereof.

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