TW202142458A - Container for low temperature storage of agricultural and horticultural crop - Google Patents

Abstract

An object of the present invention is to provide a new container and new packaging and a new preservation method that is suitable for distribution and storage that surely suppresses a significant decrease in the commercial value of agricultural and horticultural crops due to mold, and does not cause odor transfer due to antibacterial active ingredients, coloring or quality deterioration. As a solution, the present invention provides a container for low temperature storage of agricultural and horticultural crop which comprises a sustained release antibacterial film laminated having a base film layer, a volatile antibacterial active ingredient layer containing one or more selected from a group of volatile antibacterial active compounds having a boiling point in the range of 100 to 240 ℃, and a volatile antibacterial active ingredient permeable layer made of vapor deposition polymerized film in this order; and satisfies the following requirements 1 to 3.

[Requirement 1]: The volume of the storage container is 25 cm³ or more and 250,000 cm³ or less.

[Requirement 2]: The area of the sustained release antibacterial film is 1 cm² or more and 5,000 cm² or less.

[Requirement 3]: The ratio of the area of the sustained release antibacterial film (cm²) to the volume of the storage container (cm³) is 0.02 or more.

Description

Container for cryopreservation of agricultural and horticultural crops

The present invention relates to a container for low-temperature storage of agricultural and horticultural crops with a slow-release antibacterial film, a packaging body containing agricultural and horticultural crops, and a method for low-temperature storage of agricultural and horticultural crops.

In the past, long-term storage methods for agricultural and horticultural crops such as harvested common crops, vegetables, fruits, flowers, mushrooms, etc., have been known to moderately inhibit the respiration of agricultural and horticultural crops with additional deoxidizers. ; Or methods such as storage at an ice temperature below 0°C (for example, Patent Document 1, etc.), but these methods have the problem of not being able to inhibit the occurrence of mold. Domestically, pesticides such as fungicides are not approved for the distribution of agricultural and horticultural crops, and there are also significant restrictions on their use in overseas countries such as Europe. Therefore, in terms of anti-fungal methods for agricultural and horticultural crops, it is known that the harvested agricultural and horticultural crops are sterilized with a hypochlorous acid-based disinfectant in advance, but the hypochlorous acid-based disinfectant has some advantages in operation Must be highly careful and cannot obtain long-term effects.

On the other hand, with regard to methods for imparting antibacterial properties to resin films used in the packaging of agricultural and horticultural crops, there is known a method of coating the surface of the film with a vaporizing antibacterial agent (for example, Patent Document 2, etc. ), or a method of mixing an inorganic antibacterial agent or an organic antibacterial agent in a resin (for example, Patent Documents 3, 4, etc.). The former method of coating is difficult to control the vaporization from the antibacterial film The amount of antibacterial agent, if the vaporization concentration of the antibacterial agent becomes higher, not only the taste will be transferred to the agricultural and horticultural crops to be packaged, but it may also cause quality deterioration such as discoloration. In addition, the latter method of mixing produces antibacterial activity only in the part in contact with the antibacterial agent exposed on the surface of the antibacterial film, so it is difficult to show antibacterial activity to the whole packaged agricultural and horticultural crops, or the antibacterial agent system existing in the resin The antibacterial activity cannot be exerted, and in order to obtain the desired antibacterial activity, there is still a problem that a large amount of antibacterial agents are necessary.

[Prior Technical Literature]

[Patent Literature]

[Patent Document 1] JP 06-046749 A

[Patent Document 2] Japanese Patent Laid-Open No. 2006-199852

[Patent Document 3] JP 2016-030406 A

[Patent Document 4] JP 2015-189138 A

The subject of the present invention is to provide a novel container, a novel package, and a novel storage method, which can reliably inhibit the significant decrease in the commercial value of agricultural and horticultural crops caused by mold, and will not be caused by antibacterial active ingredients. In addition to the transfer of the taste, there will be no deterioration in quality such as discoloration, and it is suitable for delivery or storage.

In order to solve the above-mentioned problems, the inventor of the present invention has accumulated and dedicated research and found that by setting the volume of the storage container and the area of the sustained-release antibacterial film, and the ratio of the two to a specific range, Without degrading the quality of agricultural and horticultural crops, it is possible to reliably suppress the significant decrease in the commercial value of agricultural and horticultural crops caused by molds, thereby solving the above-mentioned problems.

Specifically, the present invention has the following matters as the gist.

1. A container for low-temperature storage of agricultural and horticultural crops, which has a slow-release antibacterial film and meets the following conditions 1 to 3; the slow-release antibacterial film is formed by sequentially stacking the following layers:

Substrate film layer;

The volatile antibacterial active ingredient layer contains more than one selected from the group of volatile antibacterial active compounds with a boiling point in the range of 100 to 240°C;

The volatile antibacterial active component penetrates the layer, which is composed of a vapor-deposited polymer film.

[Condition 1] storage containers with a volume of 25cm ³ or more 250,000cm ³ or less;

[Condition 2] The area of the sustained release antimicrobial film ² to 5,000 cm ² less than 1cm;

^{[Condition 3] The ratio of the area (cm 2} ) of the sustained-release antibacterial film to the ^{volume (cm 3} ) of the storage container is 0.02 or more.

2. An agricultural and horticultural crop storage package, which is obtained by accommodating agricultural and horticultural crops in the container for cryopreservation described in 1..

3. A method for the preservation of agricultural and horticultural crops, which uses the storage container described in 1. to store the agricultural and horticultural crops and store them at a temperature above -3°C and below 15°C.

Since the sustained-release antibacterial film provided in the container of the present invention is formed by vapor-depositing a polymer film to form a volatile antibacterial active ingredient penetration layer, it can stably release the specific volatility contained in the volatile antibacterial active ingredient layer Antibacterial active compound, by setting the volume of the storage container (condition 1) and the area of the sustained-release antibacterial film (condition 2) in a specific range, and the ratio of the area to the volume Set to a specific value or more (condition 3), and the amount of the antibacterial active ingredient volatilized in the storage container can be set to an appropriate one. The present invention can, by virtue of these additional effects, without degrading the quality of the agricultural and horticultural crops, it is possible to reliably inhibit the significant decrease in the commercial value of the agricultural and horticultural crops caused by mold.

Since the volatile antibacterial active compound with a specific boiling point range used in the present invention is also a plant aroma component used as a food additive, the present invention is useful in terms of safe preservation of agricultural and horticultural crops.

Fig. 1 is a photograph illustrating the composition 1 to 4 of the container for the preservation test in the experiment 1 of low-temperature preservation of agricultural and horticultural crops of the embodiment.

Hereinafter, the container for cryopreservation of agricultural and horticultural crops of the present invention, or a method for preserving agricultural and horticultural crops using the container will be described in detail. In addition, the "low temperature" in the present invention means a temperature range of -3°C or more and 15°C or less.

<About agricultural and horticultural crops>

The container for cryopreservation of agricultural and horticultural crops of the present invention is a container for storing agricultural and horticultural crops. Here, the agricultural and horticultural crops of the present invention refer to all cultivated crops such as ordinary crops, vegetables, fruits, flowers, mushrooms and the like. Agricultural and horticultural crops are not particularly limited, but examples include common crops such as rice, wheat, corn, taro, and beans; root vegetables such as radishes, carrots, and burdock; stem vegetables such as onions, asparagus, and angelica; Vegetable, lettuce, spinach, cabbage and other leafy vegetables, tomato, eggplant, southern Fruits and vegetables such as melon, cauliflower, broccoli, broccoli, edible chrysanthemum and other vegetables; citrus, apple, pear, grape, blueberry, strawberry, cherry, peach and other fruits; cut flowers and other flowers; mushrooms, matsutake, dance Fungus and other mushrooms.

Among them, from the standpoint of being suitable for cold-temperature storage and effectively obtaining a mold generation inhibitory effect, fruits are suitable as the agricultural and horticultural crops of the present invention, and among them, grapes, cherries, peaches, and strawberries are more suitable. For grapes, cherries, peaches, and strawberries, a temperature range of 0°C to 5°C is a more suitable storage temperature.

<About volatile antibacterial active ingredients>

In the present invention, one or more selected from the group of volatile antibacterial active compounds having a boiling point in the range of 100 to 240°C are used as volatile antibacterial active ingredients.

The compound contained in the volatile antibacterial active compound group of the present invention preferably has a carbon atom number of 5 or more and 10 or less. Specific examples of suitable compounds include Hinokitiol (boiling point: 140°C), citronellal (boiling point: 204 to 206°C), green leaf aldehyde (trans-2-hexanal, boiling point: 146°C), citronellol (boiling point: 225°C), geraniol (boiling point: 229 °C), nerol (boiling point: 224 to 225 °C/745mmHg), etc. Among them, volatile antibacterial active compounds having a boiling point in the range of 100 to 200° C. are preferred, and hinokitiol and phyllaldehyde are particularly preferred.

Regarding the formation of the volatile antibacterial active ingredient layer of the present invention, a method suitable for the form or properties of the volatile antibacterial active ingredient used can be appropriately selected. For example, if it is a volatile antibacterial active ingredient that is liquid at room temperature, it can be directly coated on one surface of the substrate film without dilution to form a volatile antibacterial active ingredient layer. Even volatile antibacterial active ingredients that are solid at room temperature or liquid at room temperature The volatile antibacterial active ingredient can also be prepared by preparing a solution with a predetermined solvent, such as ethanol, and coating it on one surface of the substrate film and allowing the solvent to evaporate, thereby forming a solution that is essentially composed of only the volatile antibacterial active ingredient. Consists of a layer of volatile antibacterial active ingredients. In addition, an evaporation treatment method can also be selected. The evaporation treatment method can be vaporized or sublimated by heating the volatile antibacterial active ingredient, and adhere to one surface of the substrate film layer, forming a volatile antibacterial Volatile antibacterial active ingredient layer composed of active ingredients.

The thickness of the volatile antibacterial active ingredient layer of the present invention may be appropriately determined according to the type of the selected volatile antibacterial active ingredient or the desired antibacterial activity maintenance period, but it is usually in the range of 1 μm to 5 mm.

<About the penetration layer of volatile antibacterial active ingredients>

The penetrating layer of the volatile antibacterial active component of the present invention is composed of a vapor-deposited polymer film. Here, the term "evaporated polymerization film" means a thin film obtained by the vapor deposition polymerization method.

The vapor-deposited polymer film generally introduces two or more raw material monomers vaporized by heating into a film-forming chamber in a vacuum state, and makes them adhere to the surface of the film-forming object arranged in the film-forming chamber. The surface is formed by polymerization between raw material monomers. That is, by forming a film while conforming to the fine irregularities existing on the surface of the volatile antibacterial active ingredient layer of the present invention, a volatile antibacterial active ingredient penetrating layer is formed, so the volatile antibacterial active ingredient layer It exhibits excellent adhesion with the penetration layer of volatile antibacterial active ingredients. In addition, the vapor-deposited polymer film can be produced even in an extremely thin film thickness, and the obtained vapor-deposited polymer film also has the advantage of containing very few impurities. Therefore, the slow-release antibacterial film of the present invention is located between the volatile antibacterial active ingredient layer and the volatile antibacterial active ingredient penetrating layer, which advantageously suppresses the occurrence of interlayer peeling due to hydrolysis or thermal shock. According to the intended amount of volatile antibacterial active ingredients, they are released and volatilized stably.

The synthetic resin system constituting the volatile antibacterial active ingredient penetration layer of the present invention can be appropriately selected from synthetic resins capable of forming a film according to a conventionally known vapor deposition polymerization method in accordance with the volatile antibacterial active ingredient. Specifically, the synthetic resin film constituting the penetration layer of the volatile antibacterial active ingredient can be exemplified by polyurea resin film, polyamide resin film, polyimide resin film, polyimide resin film, and polyester resin film. , Polyazomethine resin film, polyurethane resin film, etc. can be produced according to various synthetic resin films according to a known vapor deposition polymerization method. Among them, in particular, the polyurea resin film has a cross-linked structure through which volatile antibacterial active ingredients can effectively penetrate, so it can be advantageously used.

In addition, the thickness of the penetration layer of the volatile antibacterial active ingredient can comprehensively consider the release amount of the volatile antibacterial active ingredient to the outside of the film per unit time, or the volatile antibacterial active ingredient contained in the volatile antibacterial active ingredient layer The type or amount, etc., are appropriately determined. Specifically, the thickness of the penetrating layer of the volatile antibacterial active ingredient is preferably in the range of 0.01 μm or more and 50 μm or less, and more preferably in the range of 0.1 μm or more and 10 μm or less.

<About slow-release antibacterial film>

The sustained-release antibacterial film of the present invention preferably has a volatile antibacterial active ingredient layer formed on one surface of the base film layer, and a vapor-deposited polymer film is formed on the surface of the volatile antibacterial active ingredient layer In the aspect of the volatile antibacterial active ingredient penetrating layer, it is preferable that the size of the base film layer, the volatile antibacterial active ingredient layer, and the volatile antibacterial active ingredient penetrating layer are approximately the same.

The slow-release antibacterial film of the present invention is preferably arranged on the upper part of the container for cryopreservation of agricultural and horticultural crops, more preferably to cover the surface of the agricultural and horticultural crops inside the container, or arranged on the top surface of the container by sticking or the like. At this time, it is preferable to arrange the volatile antibacterial active ingredient penetration layer of the slow-release antibacterial film to face the agricultural and horticultural crops inside the container.

Agricultural and horticultural crops cryopreserved present invention wherein the container includes a 1cm ² of less than 5,000 cm ² film is used as an antimicrobial controlled release "condition 2." Area-based sustained release of the antimicrobial film to 5cm ² or more is preferably ² or less 1,000cm to 10cm ² or more is more preferably 500cm ² or less.

The slow-release antibacterial film provided in the container for cryopreservation of agricultural and horticultural crops of the present invention can be used in one sheet, or two or more sheets. When using a plurality of tablets, the area of the sustained-release antibacterial film in the present invention means the total area of all the sustained-release antibacterial films used.

<Containers for low-temperature storage of agricultural and horticultural crops>

The container for cryopreservation of agricultural and horticultural crops of the present invention is made of raw materials such as resin or paper, or the form of a bag shape, box shape, etc., and is not particularly limited, but a box shape is suitable among them. However, in order to maintain the amount of volatile antibacterial active ingredients volatilized from the sustained-release antibacterial film of the present invention, a container that can be sealed to a certain degree is preferred.

The container for cryopreservation of agricultural and horticultural crops of the present invention may be a container composed of the slow-release antibacterial film of the present invention, but it is simpler to include a bag composed of a material different from the slow-release antibacterial film of the present invention In the storage container for the state, the agricultural and horticultural crops are contained, and the slow-release antibacterial film of the present invention is arranged on the upper part of the accommodated agricultural and horticultural crops, which is also advantageous in terms of cost.

Agricultural and horticultural crops cryopreserved present invention wherein the container to the storage container has a volume of 25cm ³ or more ³ or less 250,000cm as "Condition 1." By volume based storage containers to 500cm ³ or more persons 100,000cm ³ or less are preferred, more 50,000cm ³ to 1000cm ³ or less are more preferred.

<About the amount of volatile antibacterial active ingredients>

In the container for low-temperature storage of agricultural and horticultural crops of the present invention, the amount of volatile antibacterial active ingredients in the storage container can be set to an appropriate value, so that the taste transfer or discoloration caused by the volatile antibacterial active ingredients will not reduce the agricultural and horticultural crops. The quality of this product can reliably inhibit the significant decrease in the commercial value of agricultural and horticultural crops caused by mold.

Generally, if a slow-release antibacterial film is placed in a closed container, the amount of antibacterial ingredients in the container will increase over time. If the amount of antibacterial ingredients in the container is reduced, sufficient antibacterial activity will not be obtained, and the agricultural and horticultural crops will be completely spoiled. On the other hand, if the amount of antibacterial ingredients in the container becomes too much, the taste of volatile antibacterial active ingredients will be transferred. Or the commodity value of agricultural and horticultural crops such as discoloration is reduced. That is, in order to reliably suppress the significant reduction in the commercial value of agricultural and horticultural crops caused by molds, and to suppress the taste transfer due to volatile antibacterial active ingredients, as well as to suppress quality deterioration such as discoloration, it is necessary to use a storage container The amount of antibacterial ingredients inside is controlled within an appropriate range.

The present invention can control the amount of antibacterial components in the storage container within an appropriate range, and identify for the first time the area of the required slow-release antibacterial film and the range of the volume of the storage container, and the slow-release antibacterial film It is related to the storage container.

The volatile antibacterial active ingredient in the present invention is one or more selected from the group of volatile antibacterial active compounds having a boiling point in the range of 100 to 240°C, and the amount of antibacterial ingredient in the storage container can be set to an appropriate range One of the conditions.

As described above, by setting the amount of the volatile antibacterial active ingredient in the container for cryopreservation of agricultural and horticultural crops of the present invention to an appropriate range, it can be a container for cryopreservation of agricultural and horticultural crops, and the container for cryopreservation of agricultural and horticultural crops can be It reliably suppresses the significant reduction in the commodity value of agricultural and horticultural crops caused by molds, and does not cause taste transfer due to antibacterial active ingredients, and does not cause quality deterioration such as discoloration, and is suitable for delivery or storage.

In order to set the amount of the volatile antibacterial active ingredient in the container for cryopreservation of agricultural and horticultural crops of the present invention to an appropriate range, the following conditions 1 to 3 must be satisfied.

[Condition 1] The volume of the vessel with a saving of 250,000cm 25cm ³ or more ³ or less.

[Condition 2] The area of the sustained release antimicrobial film ² is less than 1cm ² 5,000cm.

^{[Condition 3] The ratio of the area (cm 2} ) of the sustained-release antibacterial film to the ^{volume (cm 3} ) of the storage container is 0.02 or more.

Explain the above "Condition 3".

"Condition 3" is the ratio of the area of the sustained-release antimicrobial film (cm ² ) to the volume (cm ³ ) of the storage container, that is, the area of the sustained-release antimicrobial film (cm ² ) divided by the area of the storage container (cm 2) The value of the volume (cm ³ ) is set to 0.02 or more.

In order to set the amount of the volatile antibacterial active ingredient in the container for cryopreservation of agricultural and horticultural crops of the present invention to an appropriate range, it is particularly important to satisfy the "condition 3". When the ratio of "Condition 3" is less than 0.02, the amount of volatile antibacterial active ingredients in the container for low-temperature storage of agricultural and horticultural crops is insufficient, and the anti-fungal effect on the agricultural and horticultural crops in the storage container cannot be obtained. By setting the ratio of "Condition 3" to 0.02 or more, it can be used as a container for cold storage of agricultural and horticultural crops. The container for cold storage of agricultural and horticultural crops can surely inhibit mold-caused agricultural and horticultural crops with significant commercial value It is reduced, and there is no taste transfer caused by antibacterial active ingredients, and no quality deterioration such as discoloration occurs. It is suitable for delivery or storage.

(Example)

Hereinafter, the present invention will be explained with examples, but the technical scope of the present invention is not limited by these.

<Preparation of slow-release antibacterial film>

Using a roll-to-roll (roll to roll) method of vapor deposition polymerization film forming device, a polyethylene terephthalate (PET) film (thickness: 12μm) is used as a volatile antibacterial active compound with hinokitiol The volatile antibacterial active ingredient layer is formed so that the amount of the active ingredient becomes 1.3 mg/cm ^2. Then, the same vapor-deposited polymer film forming apparatus was used to produce a vapor-deposited polymer film made of polyurea resin. The vapor-deposited polymer film made of polyurea resin is produced with a thickness of 4 μm.

<Experiment 1 on low-temperature storage of agricultural and horticultural crops>

Use a closed container 1 with a drain box (capacity: 1,100cm ³ , bottom size: outer size 150mm×134mm, height: outer size 77mm) as a storage container. As shown in Figure 1, lay paper towels soaked in distilled water (20mL) on the bottom surface of the storage container, and place a packing sponge (2 in Figure 1) in the drain box to maintain humidity (1 in Figure 1). ^{, And use the sustained-release antibacterial film 1 (area: 78cm 2} ) (3 in Figure 1) prepared by attaching the double-sided tape to the inside of the lid of the storage container. The "condition 3" of the above-mentioned storage container in the present invention is about 0.071.

Use the fruit of Shine Muscat as a preserved agricultural and horticultural crop. As shown in Figure 1, put 12 capsules (4 in Figure 1) into the drain box with the packaging sponge, sprayed with sterilized water, and then air-dried for use. This was installed in 1 in Fig. 1 to seal the cover (3 in Fig. 1), which was taken as Example 1 and the preservation test was carried out.

The storage container with the only difference in that the sustained-release antibacterial film was not used as Comparative Example 1, and the same storage test was performed.

Observe every one week from the beginning of the preservation to confirm the spoiled fruits produced in each test area and calculate the quantity. In addition, spoiled fruit particles are removed during observation and confirmation.

The preservation test was carried out 3 times, and the average value of the cumulative spoilage rate (%) was calculated. Table 1 summarizes the test results at a storage temperature of 5°C.

As shown in Table 1, the container for cryopreservation of agricultural and horticultural crops of the present invention (Example 1) has no spoilage at all even after 70 days (2.5 months) from the start of preservation, and 91 days (3 containers) from the start of preservation The average cumulative spoilage rate after months) is 11.1%. In addition, no deterioration in quality such as taste transfer or discoloration due to the volatile antibacterial active ingredient in the fruit pieces after 91 days of storage was confirmed. In contrast, when a storage container different from that of the present invention is used (Comparative Example 1), when 21 days (three weeks) have elapsed from the start of storage, the average cumulative spoilage rate is 8.3%, and 91 days have passed (More than 3 months) reached 69.4%.

That is, by using the container for low-temperature storage of agricultural and horticultural crops of the present invention, it is possible to significantly effectively inhibit the spoilage of the expensive and high-value agricultural and horticultural crops such as the Muscarine King grapes due to mold.

On the other hand, although detailed test data is omitted, the container for low-temperature storage of agricultural and horticultural crops according to the present invention will be stored on the surface of fruit grains until the 21st day (three weeks) when stored at 20°C, which is beyond the definition of low temperature in the present invention. Brown spots appeared, and afterwards, the spoilage of all the fruit pieces was confirmed by the 49th (seven weeks). It is believed that this result suggests that even if the container for cryopreservation of agricultural and horticultural crops of the present invention is used, under conditions other than cryopreservation, the antibacterial active ingredient causes discoloration and other quality deterioration, and then promotes spoilage.

<Experiment on low-temperature storage of agricultural and horticultural crops 2>

^{Use 12 fruit pieces in the same airtight container 1 (capacity: 1,100cm 3} , bottom size: outer size 150mm×134mm, height: outer size 77mm) with a drain box as the above "Test 1 for low-temperature storage of agricultural and horticultural crops" With the sustained-release antibacterial film 1 (area: 78cm ² ), 25 fruit granules and sustained-release are used in the same container 2 (capacity: 5,000cm ³ , bottom size: outer dimension 213mm×263mm, height: outer dimension 130mm) The antibacterial film 2 (area: 312 cm ² ) was installed in the same manner as in the above-mentioned "Test 1 concerning cryopreservation of agricultural and horticultural crops".

For the preserved agricultural and horticultural crops, after spray inoculation with the Botrytis cinerea meristem suspension (106 cells/mL), the air-dried fruit of Muskharu King grapes were used to carry out a preservation test at a preservation temperature of 5°C. In addition, the container 1 (Example 2, Comparative Example 2) used distilled water (20 mL), and the container 2 (Example 3, Comparative Example 3) used distilled water (100 mL) to maintain humidity.

Observe every one week from the beginning of the preservation to confirm the spoiled fruits produced in each test area and calculate the quantity. In addition, the spoiled fruit is removed when it is observed and confirmed.

In Example 2 and Comparative Example 2, the preservation test was carried out 5 times, and in Example 3 and Comparative Example 3, the preservation test was carried out 4 times, and the average value of the cumulative spoilage rate (%) was calculated.

Table 2 summarizes the test results.

The "Test 2 on Low-temperature Storage of Agricultural and Horticultural Crops" is a storage test that simulates a very harsh environment of mandatory inoculation with Botrytis cinerea.

As shown in Table 2, in Comparative Example 2 using a storage container different from the present invention, on the 28th day of the start of preservation, all the fruit pieces were tested for spoilage. In the same way, a storage container different from the present invention was used. In Comparative Example 3, 98% of the test fruit pieces were spoiled.

On the other hand, even in the storage test under such severe conditions, in the container for low-temperature storage of agricultural and horticultural crops of the present invention (Examples 2 and 3), the spoilage of the agricultural and horticultural crops due to mold stays The test fruit was about 1/5 or less. In particular, in Example 3, the spoilage caused by mold accounted for 6% of the test fruit, and the spoilage suppression effect was obviously extremely excellent. In addition, regardless of the volume of the storage container under "Condition 1", the excellent spoilage suppression effect is clearly exhibited.

In addition, in the fruit pieces of Examples 2 and 3 after 28 days from the storage start, no deterioration in quality such as taste transfer or discoloration due to the antibacterial active ingredient was confirmed.

<Experiment on low-temperature storage of agricultural and horticultural crops 3>

^{Use fruit pellets in the same airtight container 1 with drain box (capacity: 1,100cm 3} , bottom size: outer size 150mm×134mm, height: outer size 77mm) same as the above-mentioned "Test 1 for low-temperature storage of agricultural and horticultural crops" 12 capsules, slow-release antibacterial film 1 (area: 78cm ² ), slow-release antibacterial film 3 (area: 39cm ² ), slow-release antibacterial film 4 (area: 19cm ² ), and are related to the above-mentioned "related agricultural and horticultural crops The test 1 of cryopreservation was installed in the same way, and distilled water (20 mL) was used to maintain humidity.

For the preserved agricultural and horticultural crops, after spray inoculation with the Botrytis cinerea conidia suspension (106 pcs/mL), the air-dried fruit of Musk Ching King grapes were used to carry out the preservation test at a temperature of 5°C.

Observe every one week from the beginning of the preservation to confirm the spoiled fruits produced in each test area and calculate the quantity. In addition, the spoiled fruit is removed when it is observed and confirmed.

The preservation test was carried out 4 times, and the average value of the cumulative spoilage rate (%) was calculated.

Table 3 summarizes the test results.

The "Test 3 on low-temperature storage of agricultural and horticultural crops" is a storage test that simulates a very harsh environment of mandatory inoculation with Botrytis cinerea.

As shown in Table 3, in Comparative Example 5 using a storage container different from the present invention, 97.9% of the test fruit pieces were spoiled on the 28th day from the start of storage.

On the other hand, even in the storage test under such severe conditions, in the container for low-temperature storage of agricultural and horticultural crops of the present invention (Examples 4 and 5), 70 days (2.5 months) have elapsed from the start of storage. Later, the spoilage of agricultural and horticultural crops caused by mold only stayed below about 30% of the test fruit, and the spoilage inhibition effect was obviously excellent.

On the other hand, Comparative Example 4 is the "condition 3" of the present invention, that is ^{, the ratio of the area (cm 2} ) of the sustained-release antibacterial film to the volume (cm ³ ) of the storage container is about 0.017, so it is compatible with the present invention The storage container is different. Therefore, it was confirmed that after the 28th day after the preservation of Comparative Example 4, the spoilage of agricultural and horticultural crops due to fungi progressed to about 2.5 to 4 times that of the Example.

<Experiment on low-temperature storage of agricultural and horticultural crops 4>

Put 41 fruit granules in a closed container 3 (capacity: 0.0030m ³ , bottom size: outer inch 239mm×176mm, height: outer inch 91mm) ^{with a drain box, and use slow-release antibacterial film 5 (area: 233cm 2)} ), slow-release antibacterial film 1 (area: 78cm ² ), and after spray inoculation with sterile water (no inoculation) in the same manner as the above-mentioned "Test 1 on low-temperature storage of agricultural and horticultural crops", perform Table 4 on the air-dried fruit particles Storage test at the indicated storage temperature. In addition, the container 3 uses distilled water (60 mL) to maintain humidity.

Observe every one week from the beginning of the preservation to confirm the spoiled fruits produced in each test area and calculate the quantity. In addition, the spoiled fruit is removed when it is observed and confirmed.

The preservation test was carried out 3 times, and the average value of the cumulative spoilage rate (%) was calculated.

Table 4 summarizes the test results. In addition, the results of Comparative Examples 8 and 9 were significantly different from those of Examples 6 and 7 on the 21st day of the storage start, so it was judged that there was no need to continue the observation to end the test. "-" in Table 4 means that no experimental observation has been performed.

As shown in Table 4, the containers for cryopreservation of agricultural and horticultural crops of the present invention (Examples 6 and 7) are different from those of the present invention in the non-inoculation storage of Botrytis cinerea simulating a general storage state. The container (Comparative Examples 6 and 7) clearly shows that the effect of inhibiting spoilage due to mold on agricultural and horticultural crops is extremely excellent. In addition, in the fruit pieces of Examples 6 and 7 after 28 days from the storage start, no deterioration in quality such as taste transfer or discoloration due to the antibacterial active ingredient was confirmed.

On the other hand, when stored at 23°C, which is beyond the definition of low temperature in the present invention (Comparative Examples 8 and 9), even if a slow-release antibacterial film (Comparative Example 8) is used, it is clearly not possible to obtain the inhibition of mold caused by fungi. The effect of corruption. Furthermore, in Comparative Example 8, discoloration was observed on the 10th day from the start of storage. It is believed that this result suggests that even if the container for cryopreservation of agricultural and horticultural crops of the present invention is used, under conditions other than cryopreservation, quality deterioration such as discoloration due to the antibacterial active ingredient occurs, and then spoilage is promoted.

(Industrial availability)

The sustained-release antibacterial film provided in the container of the present invention is formed by vapor-depositing a polymer film to form a volatile antibacterial active ingredient penetration layer, so it can stably release the specific volatile antibacterial contained in the volatile antibacterial active ingredient layer. Active compound, and by matching the volume of the storage container (condition 1) with the The area of the sustained-release antibacterial film (condition 2) is set to a specific range, and the ratio of the area to the volume is set to a specific value or more (condition 3) to enable volatilized antibacterial active ingredients in the storage container Set the amount to an appropriate range. With this additional effect, it is very useful to reliably suppress the significant decrease in the commercial value of the agricultural and horticultural crops caused by molds without degrading the quality of the agricultural and horticultural crops.

Claims (3)

A container for low-temperature storage of agricultural and horticultural crops is provided with a slow-release antibacterial film and meets the following conditions 1 to 3; the slow-release antibacterial film is formed by sequentially stacking the following layers: Substrate film layer; The volatile antibacterial active ingredient layer contains at least one selected from the group of volatile antibacterial active compounds with a boiling point in the range of 100 to 240°C; The penetration layer of volatile antibacterial active ingredients is composed of vapor-deposited polymer film; [Condition 1] storage containers with a volume of 25cm ³ or more 250,000cm ³ or less; [Condition 2] The area of the sustained release antimicrobial film ² to 5,000 cm ² less than 1cm; ^{[Condition 3] The ratio of the area (cm 2} ) of the sustained-release antibacterial film to the ^{volume (cm 3} ) of the storage container is 0.02 or more. An agricultural and horticultural crop storage package, which is obtained by accommodating agricultural and horticultural crops in the cryopreservation container described in claim 1. A method for preserving agricultural and horticultural crops is to use the storage container described in claim 1 to store the agricultural and horticultural crops at a temperature above -3°C and below 15°C.

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