TW202128628A - A neuromuscular blocker and preparation method thereof - Google Patents

Abstract

The present disclosure relates to a neuromuscular blocker and preparation method thereof. Specifically, the present disclosure provides a compound represented by formula I, a preparation method thereof, and use thereof for blocking neuromuscular. The definition of each group of formula I is the same as in the specification.

Description

Neuromuscular blocker and preparation method thereof

The present disclosure belongs to the field of medicine, and specifically relates to a neuromuscular blocker represented by formula I and a preparation method thereof, and its use for neuromuscular blockade.

Neuromuscular-blocking drugs (NMBD) act on neuromuscular junctions to block neuromuscular transmission and cause related muscle paralysis. Since NMBD alone does not cause loss of consciousness, such drugs are often used in combination with anesthetics in clinical practice to cooperate with tracheal intubation or cause muscle relaxation and optimize the surgical field of vision. The mode of action of NMBD mainly includes inhibiting the synthesis or release of presynaptic acetylcholine, and acting on acetylcholine receptors on the endplates of post-synaptic action fibers. According to the mechanism of action, NMBDs can be roughly divided into two categories: molecules with depolarizing activity and non-depolarizing molecules. Molecules with depolarizing activity are similar to acetylcholine, both of which can depolarize muscle fibers; however, these drugs are relatively difficult to be degraded by cholinesterase. Therefore, they can continue to depolarize the muscle fiber membranes to make the muscle fibers less effective in the follow-up. Acetylcholine's stimulation is tolerated. A typical representative is acetylcholine succinate, which is currently the only clinically available neuromuscular blocker with rapid onset and ultra-short duration (<10min). Due to the induction of continuous depolarization of muscle fibers, depolarizing molecules often cause muscle tremor and are prone to postoperative pain.

Non-depolarizing molecules occupies the majority of clinical NMBDs, mainly by binding to acetylcholine competitively with acetylcholine receptors, and do not cause muscle fiber endplate depolarization. Such drugs include rocuronium, vecuronium, galamine and so on. Unlike depolarizing molecules, most of the effects of non-depolarizing molecules can be reversed by acetylcholinesterase inhibitors.

According to different time-effects, NMBD can be divided into ultra-short-acting, short-acting, medium-acting and long-acting drugs. Among them, ultra-short-acting, short-acting, and medium-acting neuromuscular blockers are dominant in clinical practice. WO2014005122 discloses a series of non-depolarizing ultra-short-acting, short-acting, and medium-acting neuromuscular blockers, wherein the neuromuscular block (NMB) induced by the agent is reversible, for example, by administering half Cystine or related compounds are reversed.

The purpose of the present disclosure is to provide a class of non-depolarizing ultrashort-acting, short-acting or intermediate-acting neuromuscular blockers, wherein the neuromuscular blockade induced by the neuromuscular blockers can be reversed by drugs.

Therefore, the present disclosure provides a compound represented by formula I or a pharmaceutically acceptable salt thereof or their stereoisomers, rotamers or tautomers,

in,

R ₁ and R ₂ are each independently selected from hydrogen atoms and halogens;

T is selected from -CH ₂ -and -CH ₃ , wherein if T is -CH ₃ , there is no phenyl group _{with Y 1} to Y _{5 substituents;}

，其中Z₁、Z₂、Z₃、Z₄和Z₅各自獨立地選 自氫、羥基和甲氧基，或者任意兩個相鄰的Z₁、Z₂、Z₃、Z₄或Z₅共同形成亞甲二氧基或伸乙二氧基； W is selected from -CH ₃ and

, Wherein Z ₁ , Z ₂ , Z ₃ , Z ₄ and Z ₅ are each independently selected from hydrogen, hydroxyl and methoxy, or any two adjacent Z ₁ , Z ₂ , Z ₃ , Z ₄ or Z ₅ Together to form methylenedioxy or ethylenedioxy;

X ₁ , X ₂ , X ₃ and X ₄ are each independently selected from hydrogen, hydroxyl and methoxy, or any two adjacent X ₁ , X ₂ , X ₃ and X ₄ together form a methylene dioxy group or Ethylenedioxy; Y ₁ , Y ₂ , Y ₃ , Y ₄ and Y ₅ are each independently selected from hydrogen, hydroxyl and methoxy, or any two adjacent Y ₁ , Y ₂ , Y ₃ , Y ₄ or Y ₅ together form methylenedioxy or ethylenedioxy;

和

， A is selected from

with

,

in,

R ₃ and R ₄ are each independently selected from alkyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, halogen, hydroxy, mercapto, alkoxy and alkylthio, wherein the alkyl, cycloalkyl , Heterocyclyl, aryl or heteroaryl optionally further selected from alkyl, halogen, hydroxyl, mercapto, amino, pendant oxy, carboxy, nitro, cyano, alkoxy, alkylthio, ring Substituted by one or more substituents in the alkyl group, heterocyclic group, aryl group and heteroaryl group;

R ₅ and R ₆ are each independently selected from a hydrogen atom, an alkyl group, a halogen, a hydroxyl group, a mercapto group, an amino group, a carboxyl group, a nitro group, a cyano group, an alkoxy group and an alkylthio group;

Ring G _{1 is} selected from aryl and heteroaryl;

R _{7 is} selected from alkyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, halogen, hydroxy, mercapto, alkoxy and alkylthio, wherein the alkyl, cycloalkyl, heterocyclyl, aryl The group or heteroaryl group is optionally further selected from alkyl, halogen, hydroxyl, mercapto, amino, pendant oxy, carboxy, nitro, cyano, alkoxy, alkylthio, cycloalkyl, heterocyclic group , Substituted by one or more substituents in the aryl and heteroaryl groups;

R ₉ and R ₁₀ are each independently selected from a hydrogen atom, an alkyl group, a halogen, a hydroxyl group, a mercapto group, an amino group, a carboxyl group, a nitro group, a cyano group, an alkoxy group and an alkylthio group;

Ring G _{2 is} each independently selected from aryl and heteroaryl;

Ring B is a 4-8 membered heterocyclic group;

R _{8 is} selected from alkyl, halogen, hydroxyl, mercapto, amine, carboxy, nitro, cyano, alkoxy and alkylthio;

Each R _{11 is} independently selected from the group consisting of alkyl, halogen, hydroxyl, mercapto, amine, carboxy, nitro, cyano, alkoxy and alkylthio; or any two adjacent R ₁₁ and the adjacent The ring G ₁ together forms a ring M ₁ , the ring M ₁ is a fused ring aryl group or a fused heteroaryl group, wherein the ring M _{1 is} optionally further selected from alkyl, halogen, hydroxyl, mercapto, amino, pendant oxy , Carboxyl, nitro, cyano, alkoxy, alkylthio, cycloalkyl, heterocyclyl, aryl and heteroaryl substituted by one or more substituents;

Each R _{12 is} independently selected from alkyl, halogen, hydroxyl, mercapto, amine, carboxy, nitro, cyano, alkoxy and alkylthio; or any two adjacent R ₁₂ and ring G ₂ Together to form a ring M ₂ , the ring M ₂ is a fused ring aryl group or a fused heteroaryl group, wherein the ring M _{2 is} optionally further selected from alkyl, halogen, hydroxyl, mercapto, amine, pendant oxy, carboxy, Substituted by one or more substituents of nitro, cyano, alkoxy, alkylthio, cycloalkyl, heterocyclyl, aryl and heteroaryl;

m is selected from 0, 1, 2 and 3;

n is selected from 1, 2, 3, 4, 5 and 6;

p is selected from 0, 1, 2, 3, 4, 5, 6, 7 and 8;

q ₁ and q ₂ are each independently selected from 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12;

x ₁ and x ₂ are each independently selected from 0, 1, 2, 3, 4, and 5.

In certain embodiments, the compound of Formula I described in the present disclosure, or a pharmaceutically acceptable salt thereof, or a stereoisomer, rotamer, or tautomer thereof, wherein each R _{11 is} independently Selected from alkyl, halogen, hydroxy, mercapto, amine, carboxy, nitro, cyano, alkoxy and alkylthio; or any two adjacent R ₁₁ and ring G ₁ together form ring M ₁ , the Ring M ₁ is a fused ring aryl group or a fused heteroaryl group, wherein the ring M _{1 is} optionally further selected from alkyl, halogen, hydroxyl, mercapto, amine, pendant oxy, carboxy, nitro, cyano, alkyl Substituted by one or more substituents among oxy, alkylthio, cycloalkyl, heterocyclyl, aryl and heteroaryl;

Each R _{12 is} independently selected from alkyl, halogen, hydroxyl, mercapto, amine, carboxy, nitro, cyano, alkoxy and alkylthio; or any two adjacent R ₁₂ and ring G ₂ Together to form a ring M ₂ , the ring M ₂ is a fused ring aryl group or a fused heteroaryl group, wherein the ring M _{2 is} optionally further selected from alkyl, halogen, hydroxyl, mercapto, amine, pendant oxy, carboxy, One or more substituents among nitro, cyano, alkoxy, alkylthio, cycloalkyl, heterocyclic, aryl and heteroaryl groups are substituted.

In certain embodiments, R ₃ and R ₄ are both C ₁ -C ₆ alkyl groups, where the alkyl group is optionally further selected from alkyl, halogen, hydroxy, mercapto, amine, pendant oxy, carboxy, One or more substituents among nitro, cyano, alkoxy, alkylthio, cycloalkyl, heterocyclic, aryl and heteroaryl groups are substituted.

In certain embodiments, R ₅ and R ₆ are each independently selected from a hydrogen atom, a C ₁ -C ₆ alkyl group, a halogen, a hydroxyl group, and a C ₁ -C ₆ alkoxy group, and both are preferably hydrogen atoms.

In certain embodiments, q _{1 is} selected from 0 or 1.

In certain embodiments, each R _{11 is} independently selected from C ₁ -C ₆ alkyl, halogen, hydroxyl, nitro, cyano, and C ₁ -C ₆ alkoxy; or any two adjacent groups R ₁₁ and ring G ₁ together form ring M ₁ , wherein the ring M _{1 is} optionally further selected from alkyl, halogen, hydroxyl, mercapto, amino, pendant oxy, carboxy, nitro, cyano, alkoxy , Alkylthio, cycloalkyl, heterocyclyl, aryl and heteroaryl substituted by one or more substituents.

In certain embodiments, ring M _{1 is} selected from:

In certain embodiments, ring G _{1 is} selected from phenyl.

In certain embodiments, ring M _{1 is} selected from:

選自：

、

In certain embodiments, the group A in

Selected from:

,

In some embodiments, q _{1 is} not zero.

In certain embodiments, R ₃ and R ₄ are both C ₁ -C ₆ alkyl groups, wherein the alkyl group is optionally selected from alkyl, halogen, hydroxy, mercapto, amine, pendant oxy, carboxy, nitro Substituted by one or more substituents in the group, cyano group, alkoxy group, alkylthio group, cycloalkyl group, heterocyclic group, aryl group and heteroaryl group; R ₅ and R ₆ are each independently selected from hydrogen atoms , C ₁ -C ₆ alkyl, halogen, hydroxyl, C ₁ -C ₆ alkoxy, preferably all hydrogen atoms; R _{11 is} each independently selected from C ₁ -C ₆ alkyl, halogen, hydroxyl, nitro , Cyano and C ₁ -C ₆ alkoxy; or any two adjacent R ₁₁ and ring G ₁ together form a ring M ₁ , wherein the ring M _{1 is} optionally further selected from alkyl, halogen, hydroxyl, Sulfhydryl, amino, pendant oxy, carboxy, nitro, cyano, alkoxy, alkylthio, cycloalkyl, heterocyclic, aryl, and heteroaryl are substituted by one or more substituents.

選自：

、

、

In certain embodiments, R ₃ and R ₄ are both C ₁ -C ₆ alkyl groups, wherein the alkyl group is optionally selected from alkyl, halogen, hydroxy, mercapto, amine, pendant oxy, carboxy, nitro Substituted by one or more substituents of cyano group, cyano group, alkoxy group, alkylthio group, cycloalkyl group, heterocyclic group, aryl group and heteroaryl group; R ₅ and R ₆ are each independently selected from a hydrogen atom , C ₁ -C ₆ alkyl, halogen, hydroxy, C ₁ -C ₆ alkoxy, preferably hydrogen atoms; in group A

Selected from:

,

,

In certain embodiments, ring B is selected from:

,

,

,

。 In certain embodiments, ring B is selected from:

,

,

,

.

In certain embodiments, R ₇ is a C ₁ -C ₆ alkyl group, wherein the alkyl group is optionally further selected from alkyl, halogen, hydroxy, sulfhydryl, amine, pendant oxy, carboxy, nitro, cyano Is substituted by one or more substituents among the group, alkoxy group, alkylthio group, cycloalkyl group, heterocyclic group, aryl group and heteroaryl group. In certain embodiments, R ₉ and R ₁₀ are each independently selected from a hydrogen atom, a C ₁ -C ₆ alkyl group, a halogen, a hydroxyl group, and a C ₁ -C ₆ alkoxy group, and both are preferably hydrogen atoms.

In certain embodiments, q _{2 is} selected from 0, 1, or 2.

In certain embodiments, each R _{12 is} independently selected from C ₁ -C ₆ alkyl, halogen, hydroxyl, nitro, cyano, and C ₁ -C ₆ alkoxy; or any two adjacent groups R ₁₂ and ring G ₂ together form ring M ₂ , wherein the ring M _{2 is} optionally further selected from alkyl, halogen, hydroxyl, mercapto, amine, pendant oxy, carboxy, nitro, cyano, alkoxy , Alkylthio, cycloalkyl, heterocyclyl, aryl and heteroaryl substituted by one or more substituents.

In certain embodiments, ring M _{2 is} selected from:

In certain embodiments, ring G _{2 is} selected from phenyl;

In certain embodiments, ring M _{2 is} selected from:

選自：

、

In certain embodiments, the group A in

Selected from:

,

In some embodiments, q _{2 is} not zero.

In certain embodiments, R ₇ is a C ₁ -C ₆ alkyl group, wherein the alkyl group is optionally selected from alkyl, halogen, hydroxy, sulfhydryl, amine, pendant oxy, carboxy, nitro, cyano , Alkoxy, alkylthio, cycloalkyl, heterocyclyl, aryl and heteroaryl substituted by one or more substituents; R ₉ and R ₁₀ are each independently selected from hydrogen atoms, C _1- C ₆ alkyl, halogen, hydroxyl and C ₁ -C ₆ alkoxy are preferably hydrogen atoms; each R _{12 is} independently selected from C ₁ -C ₆ alkyl, halogen, hydroxyl, nitro, cyano Group and C ₁ -C ₆ alkoxy; or any two adjacent R ₁₂ and ring G ₂ together form a ring M ₂ , wherein the ring M _{2 is} optionally further selected from alkyl, halogen, hydroxyl, mercapto, Amino, pendant oxy, carboxy, nitro, cyano, alkoxy, alkylthio, cycloalkyl, heterocyclic, aryl, and heteroaryl group are substituted by one or more substituents.

,

,

,

，基團A中的

選自：

、

、

、

、

、

。 In certain embodiments, R ₇ is a C ₁ -C ₆ alkyl group, wherein the alkyl group is optionally selected from alkyl, halogen, hydroxy, sulfhydryl, amine, pendant oxy, carboxy, nitro, cyano , Alkoxy, alkylthio, cycloalkyl, heterocyclic, aryl and heteroaryl substituted by one or more substituents; R ₉ and R ₁₀ are each independently selected from hydrogen atoms, C _1- C ₆ alkyl, halogen, hydroxyl and C ₁ -C ₆ alkoxy are preferably hydrogen atoms; ring B is selected from:

,

,

,

, In group A

Selected from:

,

,

,

,

,

.

In certain embodiments, the compound may optionally be combined with a pharmaceutically acceptable anion.

The present disclosure further provides a compound represented by formula Ia or a pharmaceutically acceptable salt thereof or their stereoisomers, rotamers or tautomers,

Wherein, X ₁ -X ₄ , Y ₁ -Y ₅ , W, T, R ₁ , R ₂ , A, m, and n are defined as described above;

Q is a pharmaceutically acceptable anion, such as each independently selected from halogen, acetate, benzoate, benzenesulfonate, camphorsulfonate, citrate, ethanedisulfonate, fumarate, glucoheptonate, Gluconate, glucuronate, isethionate, lactate, lacturonate, lauryl sulfate, malate, maleate, methanesulfonate, naphthoate, naphthalenesulfonate, nitrate, hard Fatty acid, oleate, oxalate, pamoate, phosphate, hydrogen phosphate, dihydrogen phosphate, polygalacturonate, succinate, sulfate, sulfosalicylate, tartrate, tosylate , Trifluoroacetate;

y is 0.1 to 4;

z is selected from 1, 2, 3, 4;

Among them, the combination of y and z ensures that the total amount of negative charges carried by the anions in the compound is equal to the total amount of positive charges carried by the cations.

The present disclosure further provides a compound represented by formula II or a pharmaceutically acceptable salt thereof or their stereoisomers, rotamers or tautomers,

Wherein, A and n are as defined above; Y ₁ , Y ₂ , Y ₃ , Y ₄ and Y ₅ are each independently selected from a hydrogen atom, a hydroxyl group and a methoxy group.

In certain embodiments, one of Y ₁ , Y ₂ , Y ₃ , Y ₄ and Y ₅ is a methoxy group, and the remaining 4 are hydrogen atoms, for example, Y ₃ is a methoxy group; or Y ₁ , _{Two of} Y 2, Y ₃ , Y ₄ and Y ₅ are methoxy groups, and the remaining 3 are hydrogen atoms. For example, Y ₂ and Y ₃ are methoxy groups; or Y ₁ , Y ₂ , Y ₃ , Y _{3 of 4} and Y ₅ are methoxy groups, and the remaining 2 are hydrogen atoms, for example, Y ₂ , Y ₃ , and Y ₄ are methoxy groups; or Y ₁ , Y ₂ , Y ₃ , Y ₄ and Y ₅ 4 of them are methoxy groups, and the remaining 1 is a hydrogen atom. For example, Y ₁ , Y ₂ , Y ₄ , and Y ₅ are methoxy groups; or among Y ₁ , Y ₂ , Y ₃ , Y ₄ and Y ₅ Are all methoxy groups; or all of Y ₁ , Y ₂ , Y ₃ , Y ₄ and Y ₅ are hydrogen atoms.

The present disclosure further provides a compound represented by formula IIa or a pharmaceutically acceptable salt thereof or their stereoisomers, rotamers or tautomers,

Wherein, the definitions of A, n, Y ₁ , Y ₂ , Y ₃ , Y ₄ and Y ₅ are as defined in formula II;

Q is a pharmaceutically acceptable anion, such as each independently selected from halogen, acetate, benzoate, benzenesulfonate, camphorsulfonate, citrate, ethanedisulfonate, fumarate, glucoheptonate, Gluconate, glucuronate, isethionate, lactate, lacturonate, lauryl sulfate, malate, maleate, methanesulfonate, naphthoate, naphthalenesulfonate, nitrate, hard Fatty acid, oleate, oxalate, pamoate, phosphate, hydrogen phosphate, dihydrogen phosphate, polygalacturonate, succinate, sulfate, sulfosalicylate, tartrate, tosylate , Trifluoroacetate;

y is 0.1 to 4;

z is selected from 1, 2, 3, 4;

Among them, the combination of y and z ensures that the total amount of negative charges carried by the anions in the compound is equal to the total amount of positive charges carried by the cations.

In certain embodiments, the compound of formula I is selected from:

The compound may optionally be combined with a pharmaceutically acceptable anion, for example selected from halogen, acetate, benzoate, benzenesulfonate, camphorsulfonate, citrate, ethanedisulfonate, fumarate, glucoheptonate , Gluconate, glucuronate, isethionate, lactate, lacturonate, lauryl sulfate, malate, maleate, methanesulfonate, naphthoate, naphthalenesulfonate, nitrate, Stearate, oleate, oxalate, pamoate, phosphate, hydrogen phosphate, dihydrogen phosphate, polygalacturonate, succinate, sulfate, sulfosalicylate, tartrate, toluene sulfonate Acid radical, trifluoroacetate radical.

In certain embodiments, the compound of formula I is selected from:

The compound may optionally be combined with a pharmaceutically acceptable anion, for example selected from halogen, acetate, benzoate, benzenesulfonate, camphorsulfonate, citrate, ethanedisulfonate, fumarate, glucoheptonate , Gluconate, glucuronate, isethionate, lactate, lacturonate, lauryl sulfate, malate, maleate, methanesulfonate, naphthoate, naphthalenesulfonate, nitrate, Stearate, oleate, oxalate, pamoate, phosphate, hydrogen phosphate, dihydrogen phosphate, polygalacturonate, succinate, sulfate, sulfosalicylate, tartrate, toluene sulfonate Acid radical, trifluoroacetate radical.

In certain embodiments, the compound of formula Ia is selected from:

In certain embodiments, the compound of formula Ia is selected from:

Where X is selected from chlorine, bromine and iodine.

In certain embodiments, the compound of formula Ia is selected from:

In order to avoid misunderstandings, although the double bond in the compound structure in the present disclosure is drawn in some form, unless specifically specified in the name of the compound, the carbon-carbon double bond in the compound structure in the present disclosure does not specify a configuration. That is, the compounds described in the present disclosure may be E-type, Z-type or a mixture of E-type and Z-type. In certain embodiments, the compounds described in the present disclosure are E or Z forms.

The present disclosure also provides a method for preparing the aforementioned compound of formula I or a pharmaceutically acceptable salt thereof, or their stereoisomers, rotamers or tautomers, which comprises the step of mixing and reacting the compound of formula III with the compound of formula IV ,

Wherein, X ₁ -X ₄ , Y ₁ -Y ₅ , W, T, R ₁ , R ₂ , A, m, and n are defined as described above;

R _{a is} selected from hydroxyl and halogen;

R _{b is} selected from hydroxyl.

Alternatively, the form in which compound III does not form a quaternary ammonium salt structure can be reacted with the form in which compound IV does not form a quaternary ammonium salt structure, and then the quaternary ammonium salt reaction is performed.

For example, some compounds of the present disclosure can be prepared roughly in the following manner:

Wherein R is the side chain part in the compound of the present disclosure;

Or it can be prepared in the following way:

Wherein R'is a form in which the side chain part of the compound of the disclosure does not form a quaternary ammonium salt.

The present disclosure also provides a pharmaceutical composition comprising at least one of the aforementioned compounds or their pharmaceutically acceptable salts or their stereoisomers, rotamers or tautomers, and a pharmaceutically acceptable carrier, dilution Agents or excipients.

In some embodiments, the unit dose of the pharmaceutical composition is 0.001 mg-1000 mg.

In some embodiments, the pharmaceutical composition contains 0.01%-99.99% of the aforementioned compound based on the total weight of the composition. In some embodiments, the pharmaceutical composition contains 0.1%-99.9% of the aforementioned compound. In some embodiments, the pharmaceutical composition contains 0.5%-99.5% of the aforementioned compound. In some embodiments, the pharmaceutical composition contains 1%-99% of the aforementioned compound. In some embodiments, the pharmaceutical composition contains 2%-98% of the aforementioned compound.

In some embodiments, the pharmaceutical composition contains 0.01%-99.99% of a pharmaceutically acceptable carrier, diluent or excipient based on the total weight of the composition. In some embodiments, the pharmaceutical composition contains 0.1% to 99.9% of a pharmaceutically acceptable carrier, diluent or excipient. In some embodiments, the pharmaceutical composition contains 0.5%-99.5% of a pharmaceutically acceptable carrier, diluent or excipient. In some embodiments, the pharmaceutical composition contains 1%-99% of a pharmaceutically acceptable carrier, diluent or excipient. In some embodiments, the pharmaceutical composition contains 2%-98% of a pharmaceutically acceptable carrier, diluent or excipient.

The present disclosure also relates to the use of the compound or its pharmaceutically acceptable salt or their stereoisomers, rotamers or tautomers or pharmaceutical compositions containing them in the preparation of drugs for neuromuscular blockade in the above-mentioned schemes the use of.

In certain embodiments, the drug-induced neuromuscular block of the neuromuscular block can be reversed by a neuromuscular blocker antagonist. The neuromuscular blocker antagonist such as L-cysteine, D-cysteine or a mixture thereof; N-acetylcysteine; glutathione; Homocysteine; methionine; S-adenosylmethionine; or penicillamine; or a combination of the above compounds.

The present disclosure further provides a method for inducing neuromuscular block in mammals for therapeutic purposes, which comprises administering to the mammal an effective amount of the compound described in the present disclosure, or a pharmaceutically acceptable salt thereof, or their stereoisomers, rotation Isomers or tautomers or pharmaceutical compositions containing them, the mammal may be a human or non-human mammal, the mammal may be subjected to general anesthesia, and the purpose of the treatment may include surgery.

In certain embodiments, the method may further include the step of reversing the neuromuscular block in the mammal, including administering to the mammal a neuromuscular blocker antagonist. The neuromuscular blocker antagonist such as L-cysteine, D-cysteine or a mixture thereof; N-acetylcysteine; glutathione; homocysteine; methionine; S-adenosylmethionine; or penicillamine; or a combination of the above compounds.

The present disclosure further provides a kit comprising the compound described in the present disclosure, or a pharmaceutically acceptable salt thereof, or their stereoisomers, rotamers, or tautomers, or pharmaceutical compositions containing them. In certain embodiments, the kit may also include a neuromuscular blocker antagonist.

Term explanation:

Unless stated to the contrary, the terms used in the specification and the scope of the patent application have the following meanings.

The term "alkyl" refers to a saturated aliphatic hydrocarbon group, which is a straight or branched chain group containing 1 to 20 carbon atoms, preferably an alkyl group containing 1 to 12 carbon atoms. Non-limiting examples include methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, tertiary butyl, second butyl, n-pentyl, 1,1-dimethylpropyl , 1,2-dimethylpropyl, 2,2-dimethylpropyl, 1-ethylpropyl, 2-methylbutyl, 3-methylbutyl, n-hexyl, 1-ethyl- 2-methylpropyl, 1,1,2-trimethylpropyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1,3-dimethylbutyl, 2-ethylbutyl, 2-methyl Pentyl, 3-methylpentyl, 4-methylpentyl, 2,3-dimethylbutyl, n-heptyl, 2-methylhexyl, 3-methylhexyl, 4-methylhexyl, 5-methylhexyl, 2,3-dimethylpentyl, 2,4-dimethylpentyl, 2,2-dimethylpentyl, 3,3-dimethylpentyl, 2-ethyl Pentyl, 3-ethylpentyl, n-octyl, 2,3-dimethylhexyl, 2,4-dimethylhexyl, 2,5-dimethylhexyl, 2,2-dimethylhexyl, 3,3-dimethylhexyl, 4,4-dimethylhexyl, 2-ethylhexyl, 3-ethylhexyl, 4-ethylhexyl, 2-methyl-2-ethylpentyl, 2- Methyl-3-ethylpentyl, n-nonyl, 2-methyl-2-ethylhexyl, 2-methyl-3-ethylhexyl, 2,2-diethylpentyl, n-decyl, 3,3-Diethylhexyl, 2,2-Diethylhexyl, and various branched chain isomers, etc. More preferably, it is a lower alkyl group containing 1 to 6 carbon atoms. Non-limiting examples include methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, tertiary butyl, and Dibutyl, n-pentyl, 1,1-dimethylpropyl, 1,2-dimethylpropyl, 2,2-dimethylpropyl, 1-ethylpropyl, 2-methylbutyl Group, 3-methylbutyl, n-hexyl, 1-ethyl-2-methylpropyl, 1,1,2-trimethylpropyl, 1,1-dimethylbutyl, 1,2- Dimethylbutyl, 2,2-dimethylbutyl, 1,3-dimethylbutyl, 2-ethylbutyl, 2-methylpentyl, 3-methylpentyl, 4-methyl Benzylpentyl, 2,3-dimethylbutyl, etc. Alkyl groups may be substituted or unsubstituted. When substituted, the substituents may be substituted at any available attachment point. The substituents are preferably one or more of the following groups, which are independently selected from alkanes Group, alkenyl, alkynyl, alkoxy, alkylthio, alkylamino, halogen, mercapto, hydroxyl, nitro, cyano, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, ring Alkoxy, heterocycloalkoxy, cycloalkylthio, heterocycloalkylthio, pendant oxy, carboxy, or carboxylate.

The term "alkylene" refers to a saturated linear or branched aliphatic hydrocarbon group, which has two residues derived from the removal of two hydrogen atoms from the same carbon atom or two different carbon atoms of the parent alkane, which is A straight or branched chain group containing 1 to 20 carbon atoms, preferably containing 1 to 12 carbon atoms, more preferably an alkylene group containing 1 to 6 carbon atoms. Non-limiting examples of alkylene include, but are not limited to, methylene (-CH ₂ -), 1,1-ethylene (-CH(CH ₃ )-), 1,2-ethylene (-CH _{2 -)} CH ₂ )-, 1,1-propylene (-CH(CH ₂ CH ₃ )-), 1,2-propylene (-CH ₂ CH(CH ₃ )-), 1,3-propylene (-CH ₂ CH ₂ CH ₂ -), 1,4-butylene (-CH ₂ CH ₂ CH ₂ CH ₂ -), etc. The alkylene group may be substituted or unsubstituted. When substituted, the substituent may be substituted at any available point of attachment.

The term "cycloalkyl" refers to a saturated or partially unsaturated monocyclic or polycyclic cyclic hydrocarbon substituent. The cycloalkyl ring contains 3 to 20 carbon atoms, preferably 3 to 12 carbon atoms, more preferably 3 to 6 carbon atoms. Non-limiting examples of monocyclic cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl, cyclohexadienyl, cycloheptyl, cycloheptatriene Groups, cyclooctyl, etc.; polycyclic cycloalkyls include spiro, fused, and bridged cycloalkyls.

The term "spirocycloalkyl" refers to a polycyclic group that shares one carbon atom (called a spiro atom) between monocyclic rings with 5 to 20 members. It may contain one or more double bonds, but none of the rings have complete conjugation. Π electronic system. It is preferably 6 to 14 members, more preferably 7 to 10 members. According to the number of shared spiro atoms between the ring and the ring, spirocycloalkyls are classified into single spirocycloalkyls, dispirocycloalkyls or polyspirocycloalkyls, preferably monospirocycloalkyls and bispirocycloalkyls . More preferably, it is a 4-member/4-member, 4-member/5-member, 4-member/6-member, 5-member/5-member, or 5-member/6-member monospirocycloalkyl. Non-limiting examples of spirocycloalkyl groups include:

The term "fused cycloalkyl" refers to an all-carbon polycyclic group consisting of 5 to 20 members. Each ring in the system shares an adjacent pair of carbon atoms with other rings in the system. One or more rings may contain one or Multiple double bonds, but none of the rings have a fully conjugated π-electron system. It is preferably 6 to 14 members, more preferably 7 to 10 members. According to the number of constituent rings, it can be classified into bicyclic, tricyclic, tetracyclic or polycyclic fused cycloalkyl groups, preferably bicyclic or tricyclic, more preferably 5-membered/5-membered or 5-membered/6-membered bicyclic alkyl. Non-limiting examples of fused cycloalkyl groups include:

The term "bridged cycloalkyl" refers to a 5- to 20-member, all-carbon polycyclic group with any two rings sharing two carbon atoms that are not directly connected. It may contain one or more double bonds, but no ring has a complete Conjugated π electron system. It is preferably 6 to 14 members, more preferably 7 to 10 members. According to the number of constituent rings, it can be classified into bicyclic, tricyclic, tetracyclic or polycyclic bridged cycloalkyls, preferably bicyclic, tricyclic or tetracyclic, more preferably bicyclic or tricyclic. Non-limiting examples of bridged cycloalkyl groups include:

The cycloalkyl ring can be fused to an aryl, heteroaryl or heterocycloalkyl ring, wherein the ring connected with the parent structure is a cycloalkyl group, non-limiting examples include indanyl, tetrahydronaphthyl , Benzocycloheptanyl, etc. Cycloalkyl groups may be optionally substituted or unsubstituted. When substituted, the substituents are preferably one or more of the following groups, which are independently selected from alkyl, alkenyl, alkynyl, alkoxy, Alkylthio, alkylamino, halogen, mercapto, hydroxyl, nitro, cyano, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, cycloalkoxy, heterocycloalkoxy, cycloalkane Thio group, heterocycloalkylthio group, pendant oxy group, carboxyl group or carboxylate group.

The term "heterocyclyl" refers to a saturated or partially unsaturated monocyclic or polycyclic cyclic hydrocarbon substituent containing 3 to 20 ring atoms, one or more of which is selected from nitrogen, oxygen or S(O) _m (where m is an integer of 0 to 2) heteroatoms, but does not include the ring part of -OO-, -OS- or -SS-, and the remaining ring atoms are carbon. It preferably contains 3 to 12 ring atoms, of which 1 to 4 are heteroatoms; more preferably, it contains 3 to 6 ring atoms. Non-limiting examples of monocyclic heterocyclic groups include pyrrolidinyl, imidazolidinyl, tetrahydrofuranyl, tetrahydrothienyl, dihydroimidazolyl, dihydrofuranyl, dihydropyrazolyl, dihydropyrrolyl, piperidine Group, piperazinyl, morpholinyl, thiomorpholinyl, homopiperazinyl, etc., preferably piperidinyl and pyrrolidinyl. Polycyclic heterocyclic groups include spiro, fused, and bridged heterocyclic groups.

The term "spiroheterocyclic group" refers to a polycyclic heterocyclic group sharing one atom (called a spiro atom) between monocyclic rings of 5 to 20 members, wherein one or more ring atoms are selected from nitrogen, oxygen or S(O ) _{Heteroatoms of m} (where m is an integer of 0 to 2), and the remaining ring atoms are carbon. It can contain one or more double bonds, but none of the rings have a fully conjugated π-electron system. It is preferably 6 to 14 members, more preferably 7 to 10 members. According to the number of spiro atoms shared between the ring and the ring, the spiro heterocyclic group is divided into a single spiro heterocyclic group, a dispiro heterocyclic group or a polyspiro heterocyclic group, preferably a single spiro heterocyclic group and a dispiro heterocyclic group . More preferably, it is 4 members/4 members, 4 members/5 members, 4 members/6 members, 5 members/5 members or 5 members/6 members monospiro heterocyclic groups. Non-limiting examples of spiroheterocyclic groups include:

The term "fused heterocyclic group" refers to a polycyclic heterocyclic group with 5 to 20 members. Each ring in the system shares an adjacent pair of atoms with other rings in the system. One or more rings may contain one or more Double bond, but none of the rings have a fully conjugated π-electron system, where one or more of the ring atoms are heteroatoms selected from nitrogen, oxygen or S(O) _m (where m is an integer from 0 to 2), and the rest of the ring The atom is carbon. It is preferably 6 to 14 members, more preferably 7 to 10 members. According to the number of constituent rings, it can be divided into bicyclic, tricyclic, tetracyclic or polycyclic fused heterocyclic groups, preferably bicyclic or tricyclic, more preferably 5-membered/5-membered or 5-membered/6-membered bicyclic fused heterocyclic group . Non-limiting examples of fused heterocyclic groups include:

The term "bridged heterocyclic group" refers to a polycyclic heterocyclic group with 5 to 14 members, and any two rings share two atoms that are not directly connected. It may contain one or more double bonds, but none of the rings has a complete common A conjugated π-electron system in which one or more ring atoms are heteroatoms selected from nitrogen, oxygen or S(O) _m (where m is an integer of 0 to 2), and the remaining ring atoms are carbon. It is preferably 6 to 14 members, more preferably 7 to 10 members. According to the number of constituent rings, it can be classified into bicyclic, tricyclic, tetracyclic or polycyclic bridged heterocyclic groups, preferably bicyclic, tricyclic or tetracyclic, more preferably bicyclic or tricyclic. Non-limiting examples of bridged heterocyclic groups include:

The heterocyclyl ring may be fused to an aryl, heteroaryl or cycloalkyl ring, wherein the ring connected to the parent structure is a heterocyclic group, non-limiting examples of which include:

和

等。

with

Wait.

The heterocyclic group may be optionally substituted or unsubstituted. When substituted, the substituent is preferably one or more of the following groups, which are independently selected from alkyl, alkenyl, alkynyl, alkoxy, Alkylthio, alkylamino, halogen, mercapto, hydroxyl, nitro, cyano, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, cycloalkoxy, heterocycloalkoxy, cycloalkane Thio group, heterocycloalkylthio group, pendant oxy group, carboxyl group or carboxylate group.

The term "aryl" refers to a 6 to 14-membered all-carbon monocyclic or fused polycyclic (that is, rings sharing adjacent pairs of carbon atoms) group with a conjugated π-electron system, preferably 6 to 10 members, for example Phenyl and naphthyl. The aryl ring can be fused to a heteroaryl, heterocyclic or cycloalkyl ring, wherein the ring connected to the parent structure is an aryl ring, non-limiting examples of which include:

The aryl group may be substituted or unsubstituted. When substituted, the substituent is preferably one or more of the following groups, which are independently selected from the group consisting of alkyl, alkenyl, alkynyl, alkoxy, and alkylthio , Alkylamino, halogen, mercapto, hydroxyl, nitro, cyano, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, cycloalkoxy, heterocycloalkoxy, cycloalkylthio, The heterocycloalkylthio group, carboxyl group or carboxylate group is preferably phenyl.

The term "fused ring aryl" can be an unsaturated aromatic fused ring structure containing 8-14 ring atoms and two or more ring structures sharing two adjacent atoms connected to each other. The number of atoms is preferably 8-12. For example, it includes all unsaturated fused ring aryl groups, such as naphthalene, phenanthrene, etc., and also includes partially saturated fused ring aryl groups, such as benzo 3-8 membered saturated monocyclic cycloalkyl, benzo 3-8 membered partially saturated monocyclic ring Specific examples of the alkyl group are 2,3-dihydro-1H-indenyl, IH-indenyl, 1,2,3,4-tetrahydronaphthyl, 1,4-dihydronaphthyl and the like.

The term "heteroaryl" refers to a heteroaromatic system containing 1 to 4 heteroatoms and 5 to 14 ring atoms, where the heteroatoms are selected from oxygen, sulfur, and nitrogen. Heteroaryl groups are preferably 5 to 12 members, such as imidazolyl, furyl, thienyl, thiazolyl, pyrazolyl, oxazolyl, pyrrolyl, tetrazolyl, pyridyl, pyrimidinyl, thiadiazole, pyridine Azinyl and the like are preferably imidazolyl, pyrazolyl, pyrimidinyl or thiazolyl; more preferably pyrazolyl or thiazolyl. The heteroaryl ring can be fused to an aryl, heterocyclic or ring On the alkyl ring, where the ring connected to the parent structure is a heteroaryl ring, non-limiting examples include:

Heteroaryl groups may be optionally substituted or unsubstituted. When substituted, the substituents are preferably one or more of the following groups, which are independently selected from alkyl, alkenyl, alkynyl, alkoxy, Alkylthio, alkylamino, halogen, mercapto, hydroxyl, nitro, cyano, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, cycloalkoxy, heterocycloalkoxy, cycloalkane Thio group, heterocycloalkylthio group, carboxyl group or carboxylate group.

The term "fused heteroaryl" can be an unsaturated group containing 5-14 ring atoms (including at least one heteroatom) formed by two or more ring structures sharing two adjacent atoms. Aromatic fused ring structure, including carbon atom, nitrogen atom and sulfur atom, preferably "5-12 membered fused heteroaryl", "7-12 membered fused heteroaryl", "9-12 membered fused heteroaryl" Group" etc., such as benzofuranyl, benzisofuranyl, benzothienyl, indolyl, isoindole, benzoxazolyl, benzimidazolyl, indazolyl, benzotriazolyl, Quinolinyl, 2-quinolinone, 4-quinolinone, 1-isoquinolinone, isoquinolinyl, acridinyl, phenanthridinyl, benzopyridazinyl, phthalazinyl, quinazolinyl , Quinoxalinyl, phenazinyl, pteridine, purinyl, naphthyridinyl, phenazine, phenothiazine, etc.

The fused heteroaryl group may be optionally substituted or unsubstituted. When substituted, the substituent is preferably one or more of the following groups, which are independently selected from alkyl, alkenyl, alkynyl, and alkoxy , Alkylthio, alkylamino, halogen, mercapto, hydroxyl, nitro, cyano, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, cycloalkoxy, heterocycloalkoxy, ring Alkylthio, heterocycloalkylthio, carboxyl or carboxylate.

The term "alkoxy" refers to -O- (alkyl) and -O- (unsubstituted cycloalkyl), where the definition of alkyl is as described above. Non-limiting examples of alkoxy groups include: methoxy, ethoxy, propoxy, butoxy, cyclopropoxy, cyclobutoxy, cyclopentyloxy, cyclohexyloxy. The alkoxy group may be optionally substituted or unsubstituted. When substituted, the substituent is preferably one or more of the following groups, which are independently selected from alkyl, alkenyl, alkynyl, alkoxy, Alkylthio, alkylamino, halogen, mercapto, hydroxyl, nitro, cyano, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, cycloalkoxy, heterocycloalkoxy, cycloalkane Thio group, heterocycloalkylthio group, carboxyl group or carboxylate group.

The term "alkylthio" refers to -S- (alkyl) and -S- (unsubstituted cycloalkyl), where the definition of alkyl is as described above. Non-limiting examples of alkylthio include methylthio, ethylthio, propylthio, butylthio, cyclopropylthio, cyclobutylthio, cyclopentylthio, cyclohexylthio. The alkylthio group may be optionally substituted or unsubstituted. When substituted, the substituent is preferably one or more of the following groups, which are independently selected from alkyl, alkenyl, alkynyl, alkoxy, Alkylthio, alkylamino, halogen, mercapto, hydroxyl, nitro, cyano, cycloalkyl, heterocycloalkyl, aryl, heteroaryl, cycloalkoxy, heterocycloalkoxy, cycloalkane One or more substituents in the thio group and heterocycloalkylthio group are substituted.

The term "hydroxyalkyl" refers to an alkyl group substituted with a hydroxy group, where the alkyl group is as defined above.

The term "haloalkyl" refers to an alkyl substituted by halogen, where alkyl is as defined above.

The term "deuterated alkyl" refers to an alkyl group substituted with a deuterium atom, where the alkyl group is as defined above.

The term "hydroxy" refers to the -OH group.

The term "mercapto" refers to the -SH group.

The term "pendant oxy" refers to the =0 group. For example, a carbon atom and an oxygen atom are connected by a double bond, which forms a ketone or aldehyde group.

The term "pendant thio" refers to the =S group. For example, a carbon atom and a sulfur atom are connected by a double bond to form a thiocarbonyl group -C(S)-.

The term "halogen" refers to fluorine, chlorine, bromine or iodine.

The term "amino" refers to -NH ₂ .

The term "cyano" refers to -CN.

The term "nitro" refers to -NO ₂ .

The term "carboxy" refers to -C(O)OH.

The term "aldehyde group" refers to -CHO.

The term "carboxylate group" refers to -C(O)O(alkyl) or -C(O)O(cycloalkyl), wherein alkyl and cycloalkyl are as defined above.

The term "halo" refers to a compound containing a -C(O)-halogen group.

"Optional" or "optionally" means that the event or environment described later can but does not have to occur, and the description includes occasions where the event or environment occurs or does not occur. For example, "heterocyclic group optionally substituted by an alkyl group" means that an alkyl group may but does not have to be present, and the description includes the case where the heterocyclic group is substituted by an alkyl group and the case where the heterocyclic group is not substituted by an alkyl group. .

"Substituted" refers to one or more hydrogen atoms in the group, preferably at most 5, and more preferably 1 to 3 hydrogen atoms are independently substituted with a corresponding number of substituents. It goes without saying that the substituents are only in their possible chemical positions, and those skilled in the art can determine (by experiment or theory) possible or impossible substitutions without too much effort.

”並未指定構型，即 鍵“

”可以為“

”或“

”，或者同時包含“

”和“

”兩種構型。本公開所 述化合物的化學結構中，鍵“

”並未指定構型，即鍵“

”的構型可以 為E型或Z型，或者同時包含E和Z兩種構型。 In the chemical structure of the compound described in the present disclosure, the bond "

"The configuration is not specified, i.e. the key"

"Can be"

"or"

", or both "

"with"

"Two configurations. In the chemical structure of the compound described in this disclosure, the bond"

"The configuration is not specified, i.e. the key"

The configuration of "can be E-type or Z-type, or both E and Z configurations.

Although all the above structural formulas are drawn as certain isomer forms for brevity, the present disclosure may include all isomers, such as tautomers, rotamers, geometric isomers, and diastereomers. Conformers, racemates and enantiomers.

Tautomers are structural isomers of organic compounds, which are easily converted into each other through a chemical reaction called tautomerization. This reaction often results in the migration of hydrogen atoms or protons, accompanied by the conversion of single bonds and adjacent double bonds. Some common tautomeric pairs are: keto-enol, lactam-endimine. An example of a lactam-endimine balance is between A and B as shown below.

All compounds in this disclosure can be drawn as type A or type B. All tautomeric forms are within the scope of this disclosure. The naming of the compounds does not exclude any tautomers.

The present disclosure also includes compounds of the present application that are the same as those described herein, but have one or more atoms replaced by an isotope-labeled atom having an atomic weight or mass number different from the atomic weight or mass number commonly found in nature. Examples of isotopes that can be bound to the compounds of the present application include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorus, sulfur, fluorine, iodine, and chlorine, such as ² H, ³ H, ¹¹ C, ¹³ C, ¹⁴ C, ¹³ N, ¹⁵ N, ¹⁵ O, ¹⁷ O, ¹⁸ O, ³¹ P, ³² P, ³⁵ S, ¹⁸ F, ¹²³ I, ¹²⁵ I and ³⁶ Cl, etc.

The compounds of the present disclosure may contain unnatural proportions of atomic isotopes on one or more of the atoms constituting the compound. For example, compounds can be labeled with radioisotopes, such as tritium (3H), iodine-125 (125I), or C-14 ( ¹⁴ C). For another example, deuterium can be substituted for hydrogen to form deuterated drugs. The bond formed by deuterium and carbon is stronger than the bond formed by ordinary hydrogen and carbon. Compared with undeuterated drugs, deuterated drugs can reduce toxic side effects and increase drug stability. , Enhance the efficacy, extend the biological half-life of drugs and other advantages. All changes in the isotopic composition of the compounds of this application, whether radioactive or not, are included in the scope of this application.

In addition, substitution with heavier isotopes (such as deuterium (ie ² H)) can provide certain therapeutic advantages resulting from higher metabolic stability (for example, increased in vivo half-life or reduced dosage requirements), and therefore in certain situations The following may be preferable, where the deuterium substitution can be partial or complete, and partial deuterium substitution refers to the substitution of at least one hydrogen with at least one deuterium.

The following examples further describe the preparation of the compounds and pharmaceutically acceptable salts of the present disclosure, but these examples do not limit the scope of the present disclosure.

In the embodiments of the present disclosure, the experimental methods without specific conditions are usually in accordance with conventional conditions, or in accordance with the conditions suggested by the raw material or commodity manufacturers. The reagents without specific sources are the conventional reagents purchased on the market.

The structure of the compound is determined by nuclear magnetic resonance (NMR) or/and mass spectrometry (MS). The NMR shift (δ) is given in units of ^{10 -6 (ppm).} NMR was measured with Bruker AVANCE-400 nuclear magnetic instrument, and the solvent was deuterated dimethyl sulfoxide (DMSO- d ₆ ), deuterated chloroform (CDCl ₃ ), deuterated methanol (CD ₃ OD), and the internal standard was four Methyl Silane (TMS).

The measurement of MS uses Agilent 1200 /1290 DAD-6110/6120 Quadrupole MS LC/MS (manufacturer: Agilent, MS model: 6110/6120 Quadrupole MS), waters ACQuity UPLC-QD/SQD (manufacturer: waters, MS Model: waters ACQuity Qda Detector/waters SQ Detector), THERMO Ultimate 3000-Q Exactive (manufacturer: THERMO, MS model: THERMO Q Exactive). HRMS uses Aglient 6230.

Waters 2767, Waters 2767-SQ Detecor2, Shimadzu LC-20AP and Gilson-281 preparative chromatographs were used for HPLC preparation.

The thin layer chromatography silica gel plate uses Yantai Huanghai HSGF254 or Qingdao GF254 silica gel plate. The size of the silica gel plate used in thin layer chromatography (TLC) is 0.15mm~0.2mm, and the size of thin layer chromatography separation and purification products is 0.4mm. ~0.5mm.

The silica gel column chromatography generally uses Yantai Huanghai silica gel 200~300 mesh silica gel as the carrier.

The known starting materials of the present disclosure can be synthesized by or according to methods known in the art, or can be purchased from ABCR GmbH & Co.KG, Acros Organics, Aldrich Chemical Company, Accela ChemBio Inc, Companies such as Darui Chemicals.

There is no special description in the examples, and the reaction can all be carried out under an argon atmosphere or a nitrogen atmosphere.

The argon atmosphere or nitrogen atmosphere means that the reaction flask is connected to an argon or nitrogen balloon with a volume of about 1L.

The hydrogen atmosphere means that the reaction flask is connected to a hydrogen balloon with a volume of about 1L.

The pressure hydrogenation reaction uses Parr 3916EKX hydrogenator and Qinglan QL-500 hydrogen generator or HC2-SS hydrogenator.

The hydrogenation reaction is usually evacuated and filled with hydrogen, and the operation is repeated 3 times.

The microwave reaction uses a CEM Discover-S 908860 microwave reactor.

There is no special description in the examples, and the solution refers to an aqueous solution.

There are no special instructions in the examples, and the reaction temperature is room temperature, which is 20°C to 30°C.

The monitoring of the reaction progress in the examples adopts thin-layer chromatography (TLC), the developing reagent used in the reaction, the eluent system of column chromatography used to purify the compound, and the developing reagent system of thin-layer chromatography include: A : N-hexane/ethyl acetate system, B: dichloromethane/methanol system, the volume ratio of the solvent is adjusted according to the polarity of the compound, and a small amount of basic or acidic reagents such as triethylamine and acetic acid can also be added for adjustment.

Example 1

2-(3-((4-(3-(benzyldimethylammonium)propyl)-4-oxobut-2-enyl)oxy)propyl)-6,7-dimethyl Preparation of oxy-1-(4-methoxybenzyl)-2-methyl-1,2,3,4-tetrahydroisoquinolin-2-ium iodide ( 1)

Step 1: Preparation of N-(3,4-dimethoxyphenethyl)-2-(4-methoxyphenyl)acetamide

Add 2-(4-methoxyphenyl) acetic acid (10.00g, 60.18mmol) and 2-(3,4-dimethoxyphenyl-ethyl-1-amine (12.00g, 66.19mmol) to 250ml circle Into the bottom flask, add 140ml xylene. Use Dean-Stark device to azeotropically reflux at 140°C and monitor the end of the reaction by TLC. After slowly cooling to room temperature, transfer to a beaker and add 200ml n-heptane to stir. After suction filtration, filter cake The crude product was obtained by drying under reduced pressure at 45°C. After recrystallization from ethanol, 15.86 g of N-(3,4-dimethoxyphenethyl)-2-(4-methoxyphenyl)acetamide was obtained, and the yield was 80. %.

¹ H NMR(300MHz, CDCl ₃ )δ 7.11(d,J=8.8Hz,2H), 6.88(d,J=8.7Hz,2H), 6.76(d,J=8.1Hz,1H), 6.66-6.56( m, 2H), 5.38 (s, 1H), 3.93-3.83 (m, 9H), 3.51 (s, 2H), 2.71 (t, J = 6.9 Hz, 2H), 1.30 (s, 2H).

ESI-MS: m/z 330.2.

Step 2: Preparation of 6,7-dimethoxy-1-(4-methoxybenzyl)-3,4-dihydroisoquinoline

Under an argon atmosphere, N-(3,4-dimethoxyphenethyl)-2-(4-methoxyphenyl)acetamide (39.1g, 118.7mmol) was dissolved in anhydrous acetonitrile (250mL) Middle, then add three Phosphorus oxychloride (47 mL, 593.5 mmol). The mixture was stirred at 100°C, TLC monitored the completion of the reaction, and the solvent was removed under reduced pressure. The residue was dissolved in 100ml of water, slowly cooled to room temperature and then cooled in an ice bath, a large amount of light yellow solid precipitated. After suction filtration, the filter cake was rinsed with ice water and dried under reduced pressure to obtain the product 6,7-dimethoxy-1-(4-methoxybenzyl)-3,4-dihydroisoquinoline 35g, The yield was 94.69%.

¹ H NMR (300MHz, CDCl ₃ ) δ 7.29-7.21 (m, 2H), 7.00 (s, 1H), 6.88-6.81 (m, 2H), 6.68 (s, 1H), 4.01 (s, 2H), 3.91 (s, 3H), 3.84-3.68 (m, 8H), 2.68 (dd, J=8.7, 6.4 Hz, 2H).

ESI-MS: m/z 312.2.

Step 3: Preparation of 6,7-dimethoxy-1-(4-methoxybenzyl)-1,2,3,4-tetrahydroisoquinoline

Under ice bath, dissolve 6,7-dimethoxy-1-(4-methoxybenzyl)-3,4-dihydroisoquinoline (8.60g, 27.62mmol) in 50ml methanol, batchwise Sodium borohydride (1.36 g, 35.90 mmol) was added. Slowly warm up to room temperature and stir, and TLC monitors the completion of the reaction. The solvent was removed by concentration under reduced pressure, water (50 ml) was added, and the mixture was extracted with ethyl acetate. The organic phase was washed with saturated brine, dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure. The residue was separated and purified by silica gel column chromatography (extractant DCM: MeOH=100:1) to obtain 6,7-dimethoxy-1-(4-methoxybenzyl)-1, The 2,3,4-tetrahydroisoquinoline was 6.32g, and the yield was 72.9%.

¹ H NMR (300MHz, CDCl ₃ ) δ 7.22-7.15 (m, 2H), 6.93-6.85 (m, 2H), 6.66 (s, 1H), 6.61 (s, 1H), 4.12 (dd, J=9.1, 4.6Hz, 1H), 3.88 (s, 3H), 3.83 (d, J=5.8Hz, 6H), 3.29-3.11 (m, 3H), 2.97-2.66 (m, 4H).

ESI-MS: m/z 314.2.

Step 4: 3-(6,7-Dimethoxy-1-(4-methoxybenzyl)-3,4-dihydroisoquinolin-2(1H)-yl)propan-1-ol preparation

Combine 6,7-dimethoxy-1-(4-methoxybenzyl)-1,2,3,4-tetrahydroisoquinoline (1.32g, 4.21mmol) with 3-bromopropane-1- Alcohol (585.42mg, 4.21mmol) was added to a 100ml round-bottomed flask and completely dissolved with 40ml of acetonitrile, then sodium iodide (1.89g, 12.64mmol) and anhydrous potassium carbonate (1.16g, 8.42mmol) were added to the above solution. The reaction was placed at 80°C under a nitrogen atmosphere, and the end of the reaction was monitored by TLC. After the reaction was cooled to room temperature, the insoluble matter was removed by filtration. The filtrate was concentrated to dryness under reduced pressure. After dissolving in 60ml ethyl acetate, it was washed with water and saturated sodium chloride solution. The organic layer was dried over anhydrous sodium sulfate, filtered, and the filtrate was depressurized. concentrate. The residue was separated and purified by silica gel column chromatography (dichloromethane: methanol = 50:1) to obtain 3-(6,7-dimethoxy-1-(4-methoxybenzyl)-3 , 4-Dihydroisoquinoline-2(1H)-yl)propan-1-ol 1.26g, the yield was 80.53%.

¹ H NMR(300MHz,CHCl ₃ -d)δ 7.04(d,J=8.7Hz,2H), 6.89-6.81(m,2H), 6.60(s,1H), 5.90(s,1H), 4.00(dd ,J=8.2,5.4Hz,1H),3.85(d,J=7.4Hz,5H),3.81(s,3H),3.54(s,3H),3.41-3.25(m,2H),3.16(dd, J=13.3,6.3Hz,1H),2.99(dt,J=11.5,6.0Hz,3H),2.83-2.75(m,1H),2.69(d,J=3.8Hz,1H),1.83(t,J =5.4Hz,2H),1.45(s,1H).

ESI-MS: m/z 372.2.

Step 5: 4-(3-(6,7-Dimethoxy-1-(4-methoxybenzyl)-3,4-dihydroisoquinolin-2(1H)-yl)propoxy ) Preparation of 4-oxobut-2-enoic acid

Combine 3-(6,7-dimethoxy-1-(4-methoxybenzyl)-3,4-dihydroisoquinoline-2(1H)-yl)-1-propanol (1.70g , 4.4mmol) and maleic anhydride (516.05mg, 5.26mmol) were added to a 100ml round bottom flask, 50ml of acetonitrile was added to dissolve and cooled to 0℃ in an ice bath, and triethylamine (0.84ml, 5.95mmol) was slowly added dropwise After dripping, the temperature was raised to room temperature and stirred. TLC monitored the reaction to be complete. The reaction solution was concentrated to dryness under reduced pressure, dissolved in 50 ml of dichloromethane, washed with water and saturated sodium chloride solution, the organic layer was dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure. The residue was subjected to silica gel column chromatography (dichloromethane: methanol (1‰ acetic acid)=30:1) to obtain 4-(3-(6,7-dimethoxy-1-(4-methoxy) Benzyl)-3,4-dihydroisoquinoline-2(1H)-yl)propoxy)-4-oxobut-2-enoic acid 1.45g, the yield was 68.03%.

¹ H NMR(300MHz,CHCl ₃ -d)δ 7.04(d,J=8.7Hz,2H), 6.82(d,J=8.7Hz,2H), 6.61(d,J=12.3Hz,2H), 5.92 5.27(m,2H), 4.39-4.16(m,2H), 3.86(s,3H), 3.77(s,6H), 3.43(d,J=4.3Hz,3H), 3.30-2.70(m,7H) ,1.28(d,J=9.6Hz,2H).

ESI-MS: m/z 470.2.

Step 6: Preparation of 3-(benzyl(methyl)amino)propan-1-ol

Add 3-(methylamino)propan-1-ol (1.80g, 20.19mmol) and benzaldehyde (2.36g, 22.21mmol) into a 50ml round bottom flask, add 20ml of isopropanol, and stir at room temperature. Slowly add glacial acetic acid (2.43g, 40.39mmol) dropwise. After the addition is complete, add borane-5-ethyl-2-methylpyridineborane complex (1.35g, 10.10mmol) slowly and dropwise. TLC monitors the completion of the reaction. . Add saturated ethyl acetate solution of hydrogen chloride, concentrate to dryness under reduced pressure, add ethyl acetate (30ml) and water (25ml) to the residue, adjust pH<2 with 1N hydrochloric acid aqueous solution, separate the aqueous phase, separate the aqueous phase Adjust pH>9 with 2M sodium hydroxide aqueous solution, and then extract with ethyl acetate. The organic phase was washed with saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure. Remnants borrowed It was separated and purified by silica gel column chromatography (EA:PE=3:1) to obtain 3.15g of 3-(benzyl(methyl)amino)propan-1-ol with a yield of 87.75%.

¹ H NMR (300MHz, CHCl ₃ -d) δ 7.28 (dtd, J=12.4, 6.5, 6.0, 3.8Hz, 5H), 3.83-3.65 (m, 2H), 3.58-3.37 (m, 2H), 2.70- 2.55 (m, 2H), 2.29-2.11 (m, 3H), 1.72 (dd, J = 10.8, 5.6 Hz, 2H), 1.23 (pd, J = 6.9, 5.6, 3.6 Hz, 1H).

ESI-MS: m/z 180.2.

Step 7: 3-(6,7-Dimethoxy-1-(4-methoxybenzyl)-3,4-dihydroisoquinolin-2(1H)-yl)propyl-4-chloro Preparation of -4-side oxybut-2-enoate

At 0℃, under nitrogen atmosphere, to 4-(3-(6,7-dimethoxy-1-(4-methoxybenzyl)-3,4-dihydroisoquinoline-2(1H )-Yl)propoxy)-4-oxobut-2-enoic acid (1.3g, 2.77mmol) in anhydrous 1,2-dichloroethane (40mL) was added oxalic chloride (1.1mL, 13.84mmol). The reaction system was stirred at 0°C. After the reaction, the solvent was removed from the solution in vacuum at room temperature, and then the residue was subjected to two solvent distillation cycles in vacuum using dry 1,2-dichloroethane to remove the oxalyl chloride to obtain 3-(6,7 -Dimethoxy-1-(4-methoxybenzyl)-3,4-dihydroisoquinolin-2(1H)-yl)propyl 4-chloro-4-oxobut-2-enoic acid The ester is 1.29g, and the yield is 97.06%.

Step 8: 3-(Benzyl(methyl)amino)propyl(3-(6,7-dimethoxy-1-(4-methoxybenzyl)-3,4-dihydroisoquine Preparation of lin-2(1H)-yl)propyl)maleate

Add 1.29g (2.64mmol) of 3-(6,7-dimethoxy-1-(4-methoxybenzyl)-3,4-dihydroisoquinoline-2(1H)-yl)propane 4-chloro-4-oxobut-2-enoate was dissolved in dry 1,2-dichloroethane (8mL) and added to the stirred 3-(benzyl(methyl)amine Base)-1-propanol (473.89mg, 2.64mmol) and 4A molecular sieve powder (0.3g) in a slurry of a mixture of dry dichloroethane (35mL) and acetonitrile (20mL), stirring at room temperature, TLC monitoring the end of the reaction . After vacuum filtration and removal of the solvent in vacuo, the residue was dissolved in 60 ml of ethyl acetate, washed with water and saturated sodium chloride solution respectively, the organic layer was dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure. The residue was separated and purified by silica gel column chromatography (dichloromethane: methanol=50:1) to obtain 3-(benzyl(methyl)amino)propyl (3-(6,7-dimethoxy- 1-(4-methoxybenzyl)-3,4-dihydroisoquinoline-2(1H)-yl)propyl)maleate 1.23g, the yield was 73.76%.

¹ H NMR(300MHz,CHCl3-d)δ 7.32(d,J=4.4Hz,5H), 7.07(d,J=8.5Hz,2H), 6.86-6.77(m,4H), 6.59(s,1H) ,4.29(t,J=6.5Hz,2H),4.23-4.00(m,3H), 3.87(s,3H), 3.80(s,3H), 3.67(s,3H), 3.52(s,2H), 3.07-2.99(m,1H), 2.84-2.77(m,1H), 2.68(q,J=6.0,5.2Hz,4H), 2.49(t,J=7.0Hz,4H), 2.24(s,3H) ,1.91(t,J=6.8Hz,2H),1.79-1.73(m,2H),1.28(s,1H).

ESI-MS: m/z 631.3/316.2.

Step 9: 2-(3-((4-(3-(Benzyldimethylammonium)propyl)-4-oxobut-2-enyl)oxy)propyl)-6,7 -Dimethoxy-1-(4-methoxybenzyl)-2-methyl-1,2,3,4-tetrahydroisoquinolin-2-ium iodide (Compound 1)

At room temperature, add 3-(benzyl(methyl)amino)propyl(3-(6,7-dimethoxy-1-(4-methoxybenzyl)-3,4-dihydro Isoquinoline-2(1H)-yl)propyl)maleate (150mg, 0.237mmol) was dissolved in 10ml of dry acetonitrile, methyl iodide (337.53mg, 2.37mmol) was slowly added, and the reaction was stirred. After the completion of the reaction, the solvent was removed under reduced pressure, ethyl acetate was added, ultrasonicated for 5 minutes, filtered, the filter cake was washed with ethyl acetate, and dried under reduced pressure to obtain compound 1123.60 mg with a yield of 78.65%.

¹ H NMR (300MHz, CHCl ₃ -d) δ 8.65 (s, 1H), 7.64 (s, 2H), 7.44 (t, J=6.9Hz, 3H), 7.05-6.85 (m, 4H), 6.74 (d ,J=8.3Hz,2H),6.64(d,J=6.2Hz,1H), 5.66(d,J=11.1Hz,1H), 4.97(d,J=47.9Hz,6H), 4.40(d,J =36.6Hz,4H), 4.17(s,4H), 3.96(s,3H), 3.77(d,J=35.2Hz,9H), 3.36(d,J=4.1Hz,4H), 3.20(s,4H) ),2.42(d,J=45.1Hz,4H).

HRMS (HESI ⁺ ): m/z[M] ²⁺ : 330.1880.

Example 2

2-(3-((4-((1-benzyl-1-methylpyrrolidin-1-ium-3-yl)methoxy)-4-side oxybut-2-enyl)oxy (Yl)propyl)-6,7-dimethoxy-1-(4-methoxybenzyl)-2-methyl-1,2,3,4-tetrahydroisoquinolin-2-onium iodide Preparation of compound ( 2)

Step 1: (1-Benzylpyrrolidin-3-yl)methyl(3-(6,7-dimethoxy-1-(4-methoxybenzyl)-3,4-dihydroisoquine Preparation of lin-2(1H)-yl)propyl)maleate

According to the method of step 8 in Example 1, (1-benzylpyrrolidin-3-yl)methanol (505.65mg, 2.64mmol) was used to replace 3-(benzyl(methyl)amino)propan-1-ol to obtain (1-Benzylpyrrolidin-3-yl)methyl (3-(6,7-dimethoxy-1-(4-methoxybenzyl))-3,4-dihydroisoquinoline- 2(1H)-yl)propyl)maleate 1.32g, the yield is 77.68%.

¹ H NMR(300MHz,DMSO-d6)δ 8.31(s,2H),7.31(s,1H),7.30(s,2H),7.27-7.21(m,1H),7.10(d,J=8.6Hz, 2H), 6.78 (d, J=8.6Hz, 2H), 6.69 (s, 1H), 6.61 (s, 1H), 6.45 (s, 1H), 5.75 (s, 2H), 4.14 to 3.81 (m, 4H) ), 3.69(d,J=5.3Hz,6H),3.59(s,4H),2.90-2.68(m,6H),2.57(d,J=12.4Hz,2H),2.34(s,2H),1.96 -1.83 (m, 2H), 1.54 (dq, J=39.2, 6.5 Hz, 3H), 1.32-1.13 (m, 2H).

ESI-MS: m/z 643.3/322.2.

Step 2: 2-(3-((4-((1-benzyl-1-methylpyrrolidin-1-ium-3-yl)methoxy)-4-oxobut-2-ene (Yl)oxy)propyl)-6,7-dimethoxy-1-(4-methoxybenzyl)-2-methyl-1,2,3,4-tetrahydroisoquinoline-2 -Preparation of Onium Iodide (Compound 2)

According to the method of step 9 in Example 1, (1-benzylpyrrolidin-3-yl)methyl(3-(6,7-dimethoxy-1-(4-methoxybenzyl))- 3,4-Dihydroisoquinoline-2(1H)-yl)propyl)maleate (152.34mg, 0.237mmol) replaces 3-(benzyl(methyl)amino)propyl(3-( 6,7-Dimethoxy-1-(4-methoxybenzyl))-3,4-dihydroisoquinolin-2(1H)-yl)propyl)maleate to give compound 2 116.72mg, the yield was 73.19%.

¹ H NMR(300MHz,CHCl ₃ -d)δ 7.43(s,2H), 6.86(d,J=17.7Hz,3H), 6.76(d,J=8.2Hz,3H), 6.61(d,J=19.4 Hz, 5H), 5.59 (s, 1H), 4.77 (d, J = 41.6 Hz, 2H), 4.33 (s, 4H), 3.86-3.68 (m, 9H), 3.60 (s, 3H), 3.36 (s , 6H), 3.28-2.70 (m, 8H), 2.28 (d, J=106.2 Hz, 6H).

HRMS(HESI+)m/z[M] ²⁺ : 336.1883.

Example 3

4-benzyl-2-(((4-(3-(6,7-dimethoxy-1-(4-methoxybenzyl)-2-methyl-1,2,3,4- Tetrahydroisoquinolin-2-onium-2-yl)propoxy)-4-oxobut-2-enyl)oxy)methyl)-4-methylmorpholin-4-onium iodide Preparation of compound ( 3)

Step 1: (4-Benzylmorpholin-2-yl)methyl(3-(6,7-dimethoxy-1-(4-methoxybenzyl))-3,4-dihydroiso Preparation of quinoline-2(1H)-yl)propyl)maleate

According to the method of step 8 in Example 1, replace 3-(benzyl(methyl)amino)propan-1-ol with (4-benzylmorpholin-2-yl)methanol (574.20mg, 2.64mmol), Obtain (4-benzylmorpholin-2-yl)methyl (3-(6,7-dimethoxy-1-(4-methoxybenzyl))-3,4-dihydroisoquinoline -2(1H)-yl)propyl)maleate 1.47g, the yield was 84.52%.

¹ H NMR(300MHz,DMSO-d6)δ 7.35-7.26(m,5H),7.15-7.06(m,2H),6.82-6.75(m,2H),6.70(s,2H),6.62(s,1H) ), 6.46(s,1H), 4.16(t,J=4.5Hz,2H),4.02-3.84(m,2H),3.84-3.73(m,2H),3.70(d,J=3.5Hz,8H) ,3.54(dd,J=11.3,2.6Hz,1H),3.48(s,2H),3.24-3.14(m,1H),2.91-2.64(m,6H),2.61(s,2H),2.37(dd ,J=15.6,4.8Hz,1H), 2.08(td,J=11.2,3.3Hz,1H),1.94(t,J=10.6Hz,2H),1.71-1.50(m,2H),1.24(dd, J=6.1,1.3Hz,1H).

ESI-MS: m/z 659.3/330.1.

Step 2: 4-Benzyl-2-(((4-(3-(6,7-dimethoxy-1-(4-methoxybenzyl)-2-methyl-1,2,3 ,4-Tetrahydroisoquinolin-2-yl)propoxy)-4-oxobut-2-enyl)oxy)methyl)-4-methylmorpholin-4- Preparation of onium iodide (compound 3)

According to the method of step 9 in Example 1, (4-benzylmorpholin-2-yl)methyl(3-(6,7-dimethoxy-1-(4-methoxybenzyl))- 3,4-Dihydroisoquinoline-2(1H)-yl)propyl)maleate (156.13mg, 0.237mmol) replaces 3-(benzyl(methyl)amino)propyl(3-( 6,7-Dimethoxy-1-(4-methoxybenzyl))-3,4-dihydroisoquinolin-2(1H)-yl)propyl)maleate to give compound 3 138.62mg, the yield was 84.91%.

¹ H NMR(300MHz,CHCl ₃ -d)δ 7.74(d,J=5.9Hz,2H),7.46(t,J=7.0Hz,4H),7.07(s,1H),7.03-6.87(m,3H ), 6.79-6.70(m,2H),6.65(d,J=6.6Hz,1H), 5.21(d,J=15.3Hz,2H), 4.40(d,J=37.5Hz,5H),4.06(d ,J=28.9Hz,8H),3.87-3.70(m,9H), 3.39(d,J=5.8Hz,2H), 3.36(d,J=2.0Hz,4H), 3.29(s,3H), 3.19 (s, 3H), 2.93 (s, 3H).

HRMS(HESI+)m/z[M] ²⁺ : 344.1887.

Example 4

6,7-Dimethoxy-1-(4-methoxybenzyl)-2-(3-((4-((1-(4-methoxybenzyl)-1-methylpyrrolidine -1-Onium-3-yl) methoxy)-4-side oxybut-2-enyl) oxy) propyl)-2-methyl-1,2,3,4-tetrahydroiso Preparation of quinolin-2-onium chloride ( 4)

Step 1: Preparation of 6,7-dimethoxy-1-(4-methoxybenzyl))-2-methyl-1,2,3,4-tetrahydroisoquinoline

In a 100ml round bottom flask, add aqueous formaldehyde solution (2mL, 30% w/w) and disodium hydrogen phosphite pentahydrate (1.1g, 5.1mmol), and then add 6,7-dimethoxy-1-( 4-methoxybenzyl))-1,2,3,4-tetrahydroisoquinoline (1.1g, 3.51mmol) and 1,4-dioxane, and the mixture was stirred at 80°C, and the reaction was monitored by TLC completely. Dilute with water (100 mL), then add sodium hydroxide (300 mg, 7.5 mmol). The mixture was extracted with methyl tertiary butyl ether, the organic phase was dried over anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure. The residue was separated and purified by silica gel column chromatography (extractant DCM: MeOH=500:1) , Get 6,7-dimethoxy-1-(4-methoxybenzyl))-2-methyl-1,2,3,4-tetrahydroisoquinoline 862.5mg, the yield is 74.8%.

¹ H NMR(300MHz,DMSO-d6)δ 7.09-6.96(m,2H), 6.77(dt,J=8.7,2.4Hz,2H), 6.59(d,J=3.1Hz,1H), 6.33(d, J=3.2Hz, 1H), 3.68(q, J=3.1, 2.5Hz, 6H), 3.64(s, 1H), 3.53(t, J=2.4Hz, 3H), 3.02-2.67(m, 3H), 2.65(s,1H), 2.45(d,J=12.6Hz,2H), 2.36(d,J=3.0Hz,3H).

ESI-MS: m/z 328.2.

Step 2: 3-(6,7-Dimethoxy-1-(4-methoxybenzyl))-2-methyl-1,2,3,4-tetrahydroisoquinolin-2-yl -2-yl) propyl sulfate preparation

Combine 6,7-dimethoxy-1-(4-methoxybenzyl))-2-methyl-1,2,3,4-tetrahydroisoquinoline (52.6g, 160.65mmol) and 1 ,3-Propanediol cyclic sulfate (44.38g, 321.30mmol) was fully dissolved in 450ml of acetone, the mixture was heated to 65°C for reaction, and the end of the reaction was monitored by TLC. The mixture was cooled to room temperature, filtered, and the filter cake was added with acetone ultrasonically, and filtered to obtain 3-(6,7-dimethoxy-1-(4-methoxybenzyl))-2-methyl-1,2 ,3,4-Tetrahydroisoquinolin-2-yl-2-yl)propyl sulfate 51.40g, yield 69%.

¹ H NMR(300MHz,DMSO-d6)δ 7.02-6.91(m,2H), 6.90-6.78(m,4H), 4.73-4.59(m,1H), 3.98-3.79(m,2H), 3.71(d ,J=13.0Hz,9H),3.59(dd,J=12.7,4.1Hz,2H), 3.47(s,1H), 3.37-3.26(m,4H), 3.23(s,2H),3.11-2.93( m, 3H), 2.09 (q, J=5.1, 4.7 Hz, 1H).

ESI-MS: m/z 466.2.

Step 3: 2-(3-Hydroxypropyl))-6,7-dimethoxy-1-(4-methoxybenzyl))-2-methyl-1,2,3,4-tetra Preparation of Hydroisoquinoline-2-ammonium Chloride

Acetyl chloride (71.1 mL, 78.5 g, 1.0 mol) was added dropwise to ice-cold MeOH (700 mL), and the resulting solution was stirred for 10 minutes. Add 3-(6,7-Dimethoxy-1-(4-methoxybenzyl))-2-methyl-1,2,3,4-tetrahydroisoquinolin-2-yl-2 -Yl)propyl sulfate (46.5 g, 0.10 mol), and the reaction mixture was stirred at room temperature. After the reaction, sodium bicarbonate was added until no bubbles were generated, filtered, and the solvent was removed under reduced pressure. The residue was dissolved in CHCl ₃ , the insoluble matter was removed by filtration, and the solvent was removed under reduced pressure. The residue was dissolved in water, and solid sodium chloride was added until the aqueous solution was saturated. The aqueous phase was extracted with CHCl ₃ , the organic phase was dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain 2-(3-hydroxypropyl))-6,7-dimethoxy-1-(4-methoxy Benzyl))-2-methyl-1,2,3,4-tetrahydroisoquinoline-2-ammonium chloride 34.78g, yield 90.0%.

¹ H NMR(300MHz,DMSO-d6)δ 8.30(s,1H), 6.92(dd,J=51.4,8.6Hz,5H), 5.61(d,J=11.6Hz,1H), 4.79-4.60(m, 1H), 3.95(s,1H), 3.71(d,J=3.2Hz,6H),3.66-3.55(m,2H),3.41(s,3H),3.39-3.27(m,4H),3.22(d ,J=2.0Hz,3H),3.10(h,J=6.4,5.9Hz,2H),2.99(s,1H),2.95-2.82(m,1H),1.93(p,J=5.9Hz,1H) .

ESI-MS: m/z 386.2.

Step 4: 2-(3-((3-Carboxypropenyl)oxy)propyl))-6,7-dimethoxy-1-(4-methoxybenzyl))-2-methan Preparation of 1,2,3,4-tetrahydroisoquinoline-2-ammonium chloride

At 0 ℃, to 2-(3-hydroxypropyl))-6,7-dimethoxy-1-(4-methoxybenzyl))-2-methyl-1,2,3,4 -Tetrahydroisoquinoline-2-ammonium chloride (2.4g, 5.7mmol) and maleic anhydride (0.84g, 8.5mmol) in acetonitrile (24mL) was slowly added dropwise to triethylamine (0.87mL, 6.3mmol) , Stir the reaction. After the reaction, the solvent was removed under reduced pressure. The residue was dissolved in dichloromethane, the solution was extracted with deionized water, and the pH of the aqueous solution was adjusted to <2 with 1M hydrochloric acid. In the presence of NaCl solid (24wt% NaCl aqueous solution), the aqueous solution was extracted with DCM:ACN=5:1 (80×3ml). The DCM/ACN product solution was washed with saturated sodium chloride solution and dried over anhydrous sodium sulfate. The product solution was filtered and the solvent was removed under reduced pressure. The residue was dissolved in _{a mixed solvent of H 2} O/ACN=1:1 and freeze-dried for 48 hours to obtain 2-(3-((3-carboxypropenyl)oxy) Propyl))-6,7-dimethoxy-1-(4-methoxybenzyl))-2-methyl-1,2,3,4-tetrahydroisoquinoline-2-chloride The ammonium is 2.9g, and the yield is 95%.

¹ H NMR(300MHz,DMSO-d6)δ 13.16(s,1H), 7.19-6.08(m,7H), 5.57(d,J=5.5Hz,1H), 4.66(d,J=7.2Hz,1H) ,4.09(d,J=6.1Hz,3H),3.72(t,J=2.2Hz,7H),3.53-3.27(m,6H),3.17-2.70(m,4H),2.42-2.10(m,4H ).

ESI-MS: m/z 484.2.

Step 5: 2-(3-((4-Chloro-4-oxobut-2-enyl)oxy)propyl))-6,7-dimethoxy-1-(4-methyl (Oxybenzyl))-2-methyl-1,2,3,4-tetrahydroisoquinoline-2-ammonium chloride preparation

According to the method of step 7 in Example 1, using 2-(3-((3-carboxypropenyl)oxy)propyl))-6,7-dimethoxy-1-(4-methoxybenzyl Yl))-2-methyl-1,2,3,4-tetrahydroisoquinoline-2-ammonium chloride (1.34g, 2.77mmol) instead of 4-(3-(6,7-dimethoxy -1-(4-methoxybenzyl))-3,4-dihydroisoquinolin-2(1H)-yl)propoxy))-4-oxobut-2-enoic acid to give 2- (3-((4-Chloro-4-oxobut-2-enyl)oxy)propyl))-6,7-dimethoxy-1-(4-methoxybenzyl) )-2-Methyl-1,2,3,4-tetrahydroisoquinoline-2-ammonium chloride 1.16g, the yield is 83.25%.

Step 6: Preparation of (1-(4-methoxybenzyl)pyrrolidin-3-yl)methanol

Pyrrolidin-3-ylmethanol (2.04g, 20.19mmol) and 4-methoxybenzaldehyde (3.02g, 22.21mmol) were added to a 50ml round bottom flask, dissolved in 20ml isopropanol and stirred. At room temperature, slowly add glacial acetic acid (2.43g, 40.39mmol) dropwise, 5 minutes after the completion of the dropwise addition, slowly dropwise add borane-5-ethyl-2-methylpyridineborane complex (1.35g, 10.10) mmol), the reaction is stirred. After the reaction, add saturated hydrogen chloride ethyl acetate solution, concentrate to dryness, add water (25ml) to the residue, adjust pH<2 with 1N hydrochloric acid, and wash with ethyl acetate. Taking water layer, Adjust pH>9 with 2M aqueous sodium hydroxide solution, extract with ethyl acetate, wash the organic layer with saturated sodium chloride, dry with anhydrous sodium sulfate, filter, and concentrate the filtrate under reduced pressure. The residue was separated and purified by silica gel column chromatography (EA:PE=3:1) to obtain 3.92 g of (1-(4-methoxybenzyl)pyrrolidin-3-yl)methanol, with a yield of 87.75% .

¹ H NMR (300MHz, CHCl ₃ -d) δ 7.12 (dd, J=8.7, 2.2Hz, 2H), 6.74 (dd, J=8.6, 2.1Hz, 2H), 3.96-3.88 (m, 1H), 3.67 (d,J=2.1Hz,3H),3.50-3.40(m,3H),3.39-3.31(m,1H),2.58-2.43(m,2H),2.34(td,J=7.2,3.4Hz,2H ), 2.26-2.17 (m, 1H), 1.92-1.75 (m, 1H), 1.55-1.39 (m, 1H).

ESI-MS: m/z 222.1.

Step 7: Preparation of 3-(hydroxymethyl))-1-(4-methoxybenzyl))-1-methylpyrrolidine-1-ammonium iodide

(1-(4-Methoxybenzyl)pyrrolidin-3-yl)methanol (132mg, 0.59mmol) was fully dissolved in 13ml of acetonitrile, methyl iodide (846.63mg, 5.96mmol) was added at room temperature, and the reaction was stirred To the end of the reaction. The acetonitrile was removed under reduced pressure to obtain 117.63 mg of 3-(hydroxymethyl)-1-(4-methoxybenzyl))-1-methylpyrrolidine-1-ammonium iodide, with a yield of 83.44%.

¹ H NMR(300MHz,DMSO-d6)δ 7.56-7.46(m,2H), 7.07-6.98(m,2H), 4.89(q,J=5.0Hz,1H), 4.57(d,J=26.7Hz, 2H), 3.67-3.53 (m, 2H), 3.49-3.35 (m, 4H), 2.93 (d, J=20.6Hz, 3H), 2.81-2.61 (m, 3H), 2.32-2.11 (m, 2H) ,1.88(p,J=7.5Hz,1H).

ESI-MS: m/z 236.2.

Step 8: 6,7-Dimethoxy-1-(4-methoxybenzyl)-2-(3-((4-((1-(4-methoxybenzyl)-1-methyl (Pyrrolidine-1-onium-3-yl) methoxy)-4-oxobut-2-enyl) oxy) propyl)-2-methyl-1,2,3,4- Preparation of Tetrahydroisoquinolin-2-onium Chloride (Compound 4)

The 2-(3-((4-chloro-4-oxobut-2-enyl)oxy)propyl))-6,7-dimethoxy-1-(4-methoxy Benzyl))-2-methyl-1,2,3,4-tetrahydroisoquinoline-2-ammonium chloride was dissolved in dry 1,2-dichloroethane (8mL) and added to the stirring 3-(hydroxymethyl))-1-(4-methoxybenzyl))-1-methylpyrrolidine-1-ammonium iodide (590.84mg, 2.5mmol) and 4A molecular sieve powder (0.3g) In a slurry of a mixture of dried 1,2-dichloroethane (35 mL) and acetonitrile (20 mL), the mixture was stirred at room temperature until the reaction was complete. Filter and remove the solvent under reduced pressure. The residue was dissolved in 1,2-dichloroethane and extracted with deionized water, NaCl solid (25wt% aqueous solution) was added to the aqueous phase and extracted with ACN:DCM=4:1, and the organic phase was extracted with 10% KHCO ₃ aqueous solution and Wash with saturated sodium chloride solution. The organic layer was concentrated, and the residue was separated and purified by preparative liquid chromatography (MeOH/H ₂ O: 5% to 60%). After lyophilization, 1.23 g of compound 4 was obtained, with a yield of 70.0%.

¹ H NMR (300MHz, CHCl3-d) δ 7.53 (s, 4H), 6.94-6.85 (m, 3H), 6.76 (d, J=5.7Hz, 2H), 6.64 (d, J=3.3Hz, 1H) ,5.72-5.51(m,1H),4.66(d,J=38.1Hz,3H),4.22(d,J=44.8Hz,3H),3.91-3.76(m,9H),3.73(d,J=4.4 Hz, 4H), 3.62 (d, J = 23.5 Hz, 8H), 3.41-3.30 (m, 4H), 3.27-2.97 (m, 6H), 2.93 (s, 2H), 2.08 (s, 4H).

HRMS (HESI+): m/z[M] ²⁺ : 351.19359.

Example 5

2-(3-((4-((1-(3,4-Dimethoxybenzyl)-1-methylpyrrolidin-1-ium-3-yl)methoxy)-4-oxo But-2-enyl)oxy)propyl)-6,7-dimethoxy-1-(4-methoxybenzyl)-2-methyl-1,2,3,4- Preparation of Tetrahydroisoquinolin-2-onium Chloride ( 5)

Step 1: Preparation of (1-(3,4-dimethoxybenzyl)pyrrolidin-3-yl)methanol

According to the method of step 6 in Example 4, replace 4-methoxybenzaldehyde with 3,4-dimethoxybenzaldehyde (3.69g, 22.21mmol) to obtain (1-(3,4-dimethoxybenzaldehyde) (Yl)pyrrolidin-3-yl)methanol 4.28 g (yield 82.46%).

¹ H NMR(300MHz,CHCl ₃ -d)δ 6.77(d,J=1.6Hz,1H), 6.72-6.63(m,2H), 4.08(s,1H), 3.72(d,J=8.7Hz,6H ), 3.47-3.29 (m, 4H), 2.56-2.44 (m, 2H), 2.40-2.16 (m, 3H), 1.83 (ddt, J=17.2, 8.2, 4.7 Hz, 2H).

ESI-MS: m/z 252.2.

Step 2: Preparation of 1-(3,4-Dimethoxybenzyl))-3-(hydroxymethyl))-1-methylpyrrolidine-1-ammonium iodide

According to the method of step 7 in Example 4, replace (1-(4-methoxybenzyl) with (1-(3,4-dimethoxybenzyl)pyrrolidin-3-yl)methanol (148mg, 0.59mmol) Yl)pyrrolidin-3-yl)methanol to give 1-(3,4-dimethoxybenzyl))-3-(hydroxymethyl))-1-methylpyrrolidine-1-ammonium iodide 142.3 mg (yield 90.26%).

¹ H NMR (300MHz, DMSO-d6) δ 7.21 (t, J=2.7Hz, 1H), 7.13 (dd, J=8.2, 2.1Hz, 1H), 7.06-6.99 (m, 1H), 4.94-4.79 ( m,1H),4.58(d,J=26.1Hz,2H),3.75(s,6H),3.72-3.58(m,1H),3.51-3.38(m,4H),2.97(d,J=23.1Hz , 3H), 2.84-2.60 (m, 1H), 2.34-2.10 (m, 1H), 2.03-1.79 (m, 2H).

ESI-MS: m/z 266.2.

Step 3: 2-(3-((4-((1-(3,4-Dimethoxybenzyl)-1-methylpyrrolidin-1-ium-3-yl)methoxy)-4 -Pendant oxybut-2-enyl)oxy)propyl)-6,7-dimethoxy-1-(4-methoxybenzyl)-2-methyl-1,2,3 Preparation of ,4-tetrahydroisoquinolin-2-onium chloride (compound 5)

According to the method of step 8 in Example 4, use 1-(3,4-dimethoxybenzyl))-3-(hydroxymethyl))-1-methylpyrrolidine-1-ammonium iodide (665.9mg , 2.5mmol) replacing 3-(hydroxymethyl))-1-(4-methoxybenzyl))-1-methylpyrrolidine-1-ammonium iodide. After lyophilization, compound 5 1.36g ( The yield is 74.3%).

¹ H NMR(300MHz,CHCl3-d)δ 8.13(s,2H),7.14(s,2H),6.83(ddd,J=31.7,8.1,3.6Hz,6H),6.66(t,J=5.3Hz, 1H), 5.72-5.55 (m, 1H), 4.17 (s, 6H), 3.95-3.71 (m, 15H), 3.70-3.50 (m, 7H), 3.38 (t, J=5.3Hz, 6H), 3.12 (d, J=36.4 Hz, 6H), 2.12 (s, 4H).

HRMS (HESI+): m/z[M] ²⁺ : 366.19876.

Example 6

6,7-Dimethoxy-1-(4-methoxybenzyl)-2-methyl-2-(3-((4-((1-methyl-1-(3,4,5 -Trimethoxybenzyl)pyrrolidine-1-on-3-yl)methoxy)-4-pendant oxybut-2-enyl)oxy)propyl)-1,2,3,4 -Preparation of tetrahydroisoquinolin-2-onium chloride ( 6)

Step 1: Preparation of (1-(3,4,5-trimethoxybenzyl)pyrrolidin-3-yl)methanol

According to the method of step 6 in Example 4, 3,4,5-trimethoxybenzaldehyde (4.35g, 22.21mmol) was used to replace 4-methoxybenzaldehyde to obtain (1-(3,4,5-trimethoxybenzaldehyde) (Benzyl)pyrrolidin-3-yl)methanol 5.02g (about 86.40% yield).

¹ H NMR(300MHz,CHCl ₃ -d)δ 6.47(d,J=1.7Hz,2H), 3.75(dd,J=6.9,1.7Hz,9H), 3.62(qd,J=7.1,3.2Hz,1H ),3.51-3.47(m,1H),3.46-3.37(m,3H),2.66-2.46(m,2H),2.44-2.35(m,2H),2.31-2.21(m,1H),1.96-1.82 (m, 1H), 1.59-1.44 (m, 1H).

ESI-MS: m/z 282.2.

Step 2: Preparation of 1-(3,4,5-trimethoxybenzyl))-3-(hydroxymethyl))-1-methylpyrrolidine-1-ammonium iodide

According to the method of step 7 in Example 4, (1-(3,4,5-trimethoxybenzyl)pyrrolidin-3-yl)methanol (165.9mg, 0.59mmol) replaced (1-(4-methoxybenzyl) Benzyl) pyrrolidin-3-yl) Methanol was used to obtain 144.9 mg of 1-(3,4,5-trimethoxybenzyl))-3-(hydroxymethyl))-1-methylpyrrolidine-1-ammonium iodide (yield 83.26%).

¹ H NMR(300MHz,CHCl ₃ -d)δ 6.96(d,J=2.4Hz,2H), 4.86(s,2H), 3.84(d,J=2.4Hz,9H), 3.76(d,J=2.4 Hz,4H),3.70(d,J=7.4Hz,2H),3.21(s,1H),3.10(s,3H),2.39-2.23(m,1H),2.15(d,J=14.5Hz,1H ).

ESI-MS: m/z 296.2.

Step 3: 6,7-Dimethoxy-1-(4-methoxybenzyl)-2-methyl-2-(3-((4-((1-methyl-1-(3, 4,5-trimethoxybenzyl)pyrrolidine-1-on-3-yl)methoxy)-4-oxobut-2-enyl)oxy)propyl)-1,2, Preparation of 3,4-tetrahydroisoquinolin-2-onium chloride (compound 6)

According to the method of step 8 in Example 4, 1-(3,4,5-trimethoxybenzyl))-3-(hydroxymethyl))-1-methylpyrrolidine-1-ammonium iodide (740.96 mg, 2.5mmol) replacing 3-(hydroxymethyl))-1-(4-methoxybenzyl))-1-methylpyrrolidine-1-ammonium iodide, and lyophilizing to obtain compound 6 1.18g (The yield is about 62.10%).

¹ H NMR(300MHz,CHCl ₃ -d)δ 8.54(s,2H),6.92-6.74(m,7H),6.66(d,J=3.5Hz,1H), 5.67(d,J=5.2Hz,1H ), 4.74(d,J=39.0Hz,4H), 4.26(d,J=52.4Hz,4H),3.93-3.72(m,18H),3.56(s,6H),3.38(t,J=5.6Hz) ,3H), 3.13(d,J=27.0Hz,8H), 2.39(s,4H).

HRMS(HESI+)m/z[M] ²⁺ : 381.20412.

Example 7

2-(3-((4-((1-((2,3-dihydrobenzo[b][1,4]dioxane-6-yl)methyl)-1-methyl Pyrrolidine-1-onium-3-yl)methoxy)-4-side oxybut-2-enyl)oxy)propyl)-6,7-dimethoxy-1-(4- (Methoxybenzyl)-2-methyl-1,2,3,4-tetrahydroisoquinolin-2-onium chloride ( 7 )

Step 1: Preparation of (1-((2,3-dihydrobenzo[b][1,4]dioxane-6-yl)methyl]pyrrolidin-3-yl)methanol

According to the method of step 6 in Example 4, replace 4-methoxybenzene with 2,3-dihydrobenzo[b][1,4]dioxane-6-carbaldehyde (3.65g, 22.21mmol) Formaldehyde to obtain (1-((2,3-dihydrobenzo[b][1,4]dioxane-6-yl)methyl]pyrrolidin-3-yl)methanol 4.16g (product Rate 80.77%).

¹ H NMR (300MHz, CHCl ₃ -d) δ 6.79-6.76 (m, 1H), 6.73 (s, 2H), 4.17 (s, 6H), 3.53 (dd, J=10.2, 5.3 Hz, 1H), 3.45 (d,J=3.9Hz,3H),2.58-2.51(m,1H),2.42(ddd,J=12.2,9.0,3.5Hz,2H),2.30(hept,J=5.0Hz,1H),1.91( tq, J=8.8, 5.1, 4.4 Hz, 1H), 1.61-1.49 (m, 1H).

ESI-MS: m/z 250.2.

Step 2: 1-((2,3-Dihydrobenzo[b][1,4]dioxane-6-yl)methyl))-3-(hydroxymethyl))-1- Preparation of methylpyrrolidine-1-ammonium iodide

According to the method of step 7 in Example 4, use (1-((2,3-dihydrobenzo[b][1,4]dioxane-6-yl)methyl]pyrrolidine-3- (1-(4-methoxybenzyl)pyrrolidin-3-yl)methanol (147.2mg, 0.59mmol) to give 1-((2,3-dihydrobenzo[b][1 ,4] Dioxane Cyclohexane-6-yl)methyl))-3-(hydroxymethyl))-1-methylpyrrolidine-1-ammonium iodide 138.6 mg (yield 88.51%).

¹ H NMR(300MHz,DMSO-d6)δ 7.12(d,J=2.1Hz,1H), 7.03(dd,J=8.3,2.1Hz,1H), 6.94(dd,J=8.2,2.0Hz,1H) ,4.89(s,1H),4.51(d,J=26.3Hz,2H),3.66-3.37(m,5H),3.21-3.02(m,1H),2.93(d,J=21.2Hz,3H), 2.80-2.61 (m, 2H), 2.20 (ddt, J=18.0, 13.7, 9.6 Hz, 2H), 1.87 (td, J=12.8, 11.8, 6.6 Hz, 2H).

ESI-MS: m/z 264.2.

Step 3: 2-(3-((4-((1-((2,3-dihydrobenzo[b][1,4]dioxane-6-yl)methyl)-1 -Methylpyrrolidine-1-onium-3-yl)methoxy)-4-oxobut-2-enyl)oxy)propyl)-6,7-dimethoxy-1- Preparation of (4-methoxybenzyl)-2-methyl-1,2,3,4-tetrahydroisoquinolin-2-onium chloride (Compound 7)

According to the method of step 8 in Example 4, use 1-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)methyl))-3-(hydroxymethyl) )-1-Methylpyrrolidine-1-ammonium iodide (660.86mg, 2.5mmol) instead of 3-(hydroxymethyl)-1-(4-methoxybenzyl))-1-methylpyrrolidine 1-Ammonium iodide, after lyophilization, 1.35g of compound 7 was obtained (yield 74.17%).

¹ H NMR(300MHz,DMSO- d ₆ )δ 7.13(s,1H),7.04-6.91(m,5H),6.87(dd, J =6.2,2.6Hz,4H),6.67(t, J =4.2Hz ,1H), 5.58(d, J =10.0Hz,1H), 4.58-4.33(m,3H), 4.26(d, J =12.7Hz,6H), 4.15(d, J =6.3Hz,1H),3.92 -3.78(m,1H),3.72(t, J =3.1Hz,9H),3.48(s,4H),3.23 (d, J =2.5Hz,4H),3.13(s,2H),3.00(d, J =4.5Hz,4H), 2.90(d, J =8.8Hz,3H), 2.29(d, J =51.5Hz,4H), 1.94(d, J =6.7Hz,1H).

HRMS(HESI+)m/z[M] ²⁺ : 365.19101.

Example 8

2-(3-((4-((1-(Benzo[d][1,3]dioxol-5-ylmethyl)-1-methylpyrrolidine-1-onium-3 -Yl)Methoxy)-4-Pendant oxybut-2-enyl)oxy)propyl)-6,7-Dimethoxy-1-(4-methoxybenzyl)-2 -Methyl-1,2,3,4-tetrahydroisoquinolin-2-onium chloride ( 8 ) preparation

Step 1: Preparation of benzo[d][1,3]dioxazole-5-carbaldehyde

Add 3,4-dihydroxybenzaldehyde (1.0g, 7.24mmol), dichloromethane (3.07g, 36.20mmol), anhydrous potassium carbonate (2.5g, 18.1mmol) into a 100ml round bottom flask, and then add DMF ( 35ml). Under a nitrogen atmosphere, stirring was carried out at 120°C until the reaction was completed. The reaction solution was cooled to room temperature, filtered, and the filtrate was concentrated under reduced pressure. The residue was separated and purified by silica gel column chromatography (PE:EA=30:1) to obtain 813.3mg of benzo[d][1,3]dioxazole-5-carbaldehyde (yield 74.80%).

¹ H NMR(300MHz,DMSO-d6)δ 9.87(s,1H), 7.60(tt,J=6.4,2.5Hz,1H), 7.45-7.30(m,1H), 7.19(dd,J=7.3,2.8 Hz, 1H), 6.30-6.06 (m, 2H).

ESI-MS: m/z 151.0.

Step 2: Preparation of (1-(benzo[d][1,3]dioxol-5-ylmethyl)pyrrolidin-3-yl)methanol

According to the method of step 6 in Example 4, benzo[d][1,3]dioxazole-5-carbaldehyde (3.33g, 22.21mmol) was used to replace 4-methoxybenzaldehyde to obtain (1-(benzo [d] [1,3] Dioxol-5-ylmethyl)pyrrolidin-3-yl)methanol 3.96 g (yield 75.79%).

¹ H NMR (300MHz, CHCl ₃ -d) δ 6.76 (s, 1H), 6.66 (s, 2H), 5.83 (s, 2H), 4.18 (s, 1H), 3.48 (dd, J=10.2, 5.5 Hz ,2H),3.41(d,J=6.2Hz,3H),2.63-2.48(m,2H),2.38(td,J=9.2,5.5Hz,2H),1.87(qd,J=9.1,8.5,4.1 Hz, 1H), 1.57-1.43 (m, 1H).

ESI-MS: m/z 236.1.

Step 3: 1-(Benzo[d][1,3]dioxol-5-ylmethyl))-3-(hydroxymethyl))-1-methylpyrrolidine-1-iodide Preparation of ammonium

According to the method of step 7 in Example 4, use ((1-(benzo[d][1,3]dioxol-5-ylmethyl)pyrrolidin-3-yl)methanol (139.2mg, 0.59 mmol) replace (1-(4-methoxybenzyl)pyrrolidin-3-yl)methanol to give 1-(benzo[d][1,3]dioxol-5-ylmethyl) )-3-(hydroxymethyl))-1-methylpyrrolidine-1-ammonium iodide 132.60 mg (approximately 89.84% yield).

¹ H NMR(300MHz,DMSO-d6)δ 7.17(d,J=1.6Hz,1H), 7.08(dd,J=8.0,1.8Hz,1H), 7.01(dd,J=8.0,1.9Hz,1H) ,4.88(s,1H),4.54(d,J=27.2Hz,2H),3.67-3.53(m,1H),3.52-3.34(m,5H),2.94(d,J=21.5Hz,3H), 2.79-2.60 (m, 1H), 2.21 (ddt, J=18.2, 14.2, 8.4 Hz, 3H), 1.92-1.84 (m, 1H).

ESI-MS: m/z 250.1.

Step 4: 2-(3-((4-((1-(benzo[d][1,3]dioxol-5-ylmethyl)-1-methylpyrrolidine-1- (Onium-3-yl) methoxy)-4-side oxybut-2-enyl) oxy) propyl)-6,7-dimethoxy-1-(4-methoxybenzyl) )-2-Methyl-1,2,3,4-tetrahydroisoquinolin-2-onium chloride (Compound 8)

According to the method of step 8 in Example 4, use 1-(benzo[d][1,3]dioxol-5-ylmethyl))-3-(hydroxymethyl))-1-methyl Pyrrolidine-1-iodide (625.78mg, 2.5mmol) replaces 3-(hydroxymethyl)-1-(4-methoxybenzyl))-1-methylpyrrolidine-1-ammonium iodide, After lyophilization, compound 81.16g (yield 64.80%) was obtained.

¹ H NMR (300MHz, DMSO-d6) δ 7.18 (s, 1H), 7.12-6.95 (m, 4H), 6.94-6.80 (m, 4H), 6.71-6.61 (m, 1H), 6.13-6.03 (m ,2H), 5.59(d,J=9.5Hz,1H),4.81-4.33(m,4H),4.29-4.12(m,3H), 3.64(s,10H), 3.35(s,4H), 3.23( d, J=2.2 Hz, 3H), 3.18-3.06 (m, 4H), 3.05-2.82 (m, 6H), 2.21 (d, J=7.0 Hz, 3H), 1.96 (s, 2H).

HRMS(HESI+)m/z[M] ²⁺ : 358.18316.

Example 9

2-(3-((4-((1-benzyl-1-methylpyrrolidin-1-ium-2-yl)methoxy)-4-oxobut-2-enyl)oxy Yl)propyl)-6,7-dimethoxy-1-(4-methoxybenzyl)-2-methyl-1,2,3,4-tetrahydroisoquinolin-2-onium oxide Preparation of ( 9)

Step 1: Preparation of 1-benzyl-2-(hydroxymethyl))-1-methylpyrrolidine-1-ammonium iodide

Follow the method in step 7 of Example 4, replacing (1-(4-methoxybenzyl)pyrrolidin-3-yl) with (1-benzylpyrrolidin-2-yl)methanol (112.8mg, 0.59mmol) Methanol was used to obtain 98.3 mg of 1-benzyl-2-(hydroxymethyl))-1-methylpyrrolidine-1-ammonium iodide (yield 81.21%).

¹ H NMR (300MHz, DMSO-d6) δ 7.62 (dd, J=7.5, 2.1Hz, 2H), 7.48 (dtd, J=7.3, 5.4, 2.7Hz, 3H), 4.88-4.67 (m, 3H), 3.79-3.64(m,1H),3.58-3.39(m,5H), 2.99(d,J=21.9Hz,3H), 2.71(dq,J=9.1,3.9Hz,1H),2.37-2.13(m, 1H), 2.00-1.78 (m, 1H).

ESI-MS: m/z 206.3.

Step 2: 2-(3-((4-((1-benzyl-1-methylpyrrolidin-1-ium-2-yl)methoxy)-4-oxobut-2-ene (Yl)oxy)propyl)-6,7-dimethoxy-1-(4-methoxybenzyl)-2-methyl-1,2,3,4-tetrahydroisoquinoline-2 -Preparation of Onium Chloride (Compound 9)

Follow the method of step 8 in Example 4, replacing 3-(hydroxymethyl) with 1-benzyl-2-(hydroxymethyl))-1-methylpyrrolidine-1-iodide (515.3mg, 2.5mmol) )-1-(4-Methoxybenzyl))-1-methylpyrrolidine-1-ammonium iodide, and after lyophilization, 1.16g of compound 9 is obtained (the yield is about 64.80%).

¹ H NMR(300MHz,DMSO-d6)δ 8.37(d,J=26.7Hz,3H), 7.65-7.46(m,3H), 7.17-6.62(m,6H), 5.60(s,1H), 5.15 4.51(m,10H),4.31(d,J=34.4Hz,3H),4.02-3.87(m,2H),3.81(d,J=2.7Hz,8H),3.61-3.29(m,5H), 3.20 -3.06 (m, 2H), 3.05-2.86 (m, 4H), 2.84 (s, 1H), 2.44-1.85 (m, 4H).

ESI-MS: m/z 336.2.

Example 10

2-(3-((4-(3-(dimethyl(phenyl)ammonium)propoxy)-4-oxobut-2-enyl)oxy)propyl)-6,7 -Dimethoxy-1-(4-methoxybenzyl)-2-methyl-1,2,3,4-tetrahydroisoquinolin-2-ium chloride ( 10 )

Step 1: Preparation of N-(3-hydroxypropyl))-N,N-dimethylphenylamine iodide

According to the method of step 7 in Example 4, replace (1-(4-methoxybenzyl)pyrrolidin-3-yl)methanol with 3-(anilino)propan-1-ol (89.2mg, 0.59mmol), 67.2 mg of N-(3-hydroxypropyl))-N,N-dimethylphenylamine iodide was obtained (yield 63.2%).

¹ H NMR (300MHz, DMSO- d ₆ ) δ 7.67 (d, J = 8.1 Hz, 2H), 7.55-7.49 (m, 3H), 3.61 (s, 4H), 3.41-3.32 (m, 3H), 3.21 (s, 3H), 1.60-1.34 (m, 3H).

ESI-MS: m/z 180.1.

Step 2: 2-(3-((4-(3-(Dimethyl(phenyl)ammonium)propoxy)-4-oxobut-2-enyl)oxy)propyl)- 6,7-Dimethoxy-1-(4-methoxybenzyl)-2-methyl-1,2,3,4-tetrahydroisoquinolin-2-onium chloride (Compound 10) preparation

According to the method of step 8 in Example 4, replace 3-(hydroxymethyl))-1 with N-(3-hydroxypropyl))-N,N-dimethylphenylamine iodide (450.3mg, 2.5mmol) -(4-Methoxybenzyl))-1-methylpyrrolidine-1-ammonium iodide, and lyophilized to obtain 567.7 mg of compound 10 (yield: 28.3%).

¹ H NMR(300MHz,DMSO- d ₆ )δ 8.33(s,1H), 7.62-7.49(m,5H), 7.04-6.82(m,7H), 4.60(s,3H), 4.32(dt, J = 31.8,5.3Hz,5H), 3.87(s,2H), 3.73(d, J =3.9Hz,9H), 3.37(s,4H), 3.16(d, J =7.4Hz,2H),3.02(d, J = 6.7 Hz, 8H), 2.31 (d, J = 48.1 Hz, 4H).

ESI-MS: m/z 323.2.

Example 11

6,7-Dimethoxy-1-(4-methoxybenzyl)-2-methyl-2-(3-((4-((1-methyl-1-phenethylpyrrolidine- 1-Onium-3-yl)methoxy)-4-side oxybut-2-enyl)oxy)propyl)-1,2,3,4-tetrahydroisoquinolin-2-ium Preparation of iodide ( 11)

Step 1: Preparation of (1-phenethylpyrrolidin-3-yl)methanol

At room temperature, dissolve 2-phenylacetaldehyde (317.2mg, 2.64mmol) and pyrrolidin-3-ylmethanol (267.03mg, 2.64mmol) in isopropanol, slowly add 235mml of acetic acid, and then add again after five minutes Isopropanol. After the reaction, the pH of the reaction solution was adjusted to about 3 with hydrochloric acid. After the isopropanol was removed by concentration under reduced pressure, water was added to adjust to pH<2. After extraction with ethyl acetate, the aqueous layer was taken, and the aqueous layer was adjusted with sodium hydroxide solution. To pH>10, extract with dichloromethane, and concentrate the organic phase under reduced pressure. The residue is separated and purified by silica gel column chromatography (dichloromethane: methanol=50:1) to obtain (1-phenethylpyrrolidine). But-3-yl)methanol 449.86mg, the yield was 83%.

¹ H NMR(400MHz,CHCl ₃ -d)δ 7.21(q,J=1.3Hz,1H), 7.20(s,1H), 7.15-7.12(m,2H), 7.11(d,J=3.3Hz,1H ),3.61(dd,J=10.0,4.3Hz,1H), 3.45(dd,J=10.0,5.0Hz,1H), 2.87-2.56(m,7H), 2.48(dd,J=9.1,6.9Hz, 1H), 2.28(td,J=9.0,7.0Hz,2H),1.94(m,J=13.3,9.2,4.3Hz,1H),1.63(m,J=12.7,8.5,7.1,4.1Hz,1H) .

ESI-MS: m/z 206.2.

Step 2: 3-(6,7-Dimethoxy-1-(4-methoxybenzyl))-3,4-dihydroisoquinolin-2(1H)-yl)propyl((1 -Phenethylpyrrolidin (but-3-yl) methyl) maleate preparation

1.29g (2.64mmol) 3-(6,7-dimethoxy-1-(4-methoxybenzyl))-3,4-dihydroisoquinoline-2(1H)-yl)propane 4-chloro-4-oxobut-2-enoate was dissolved in dry 1,2-dichloroethane (8mL) and added to the stirred (1-phenethylpyrrolidine but-2-enoate) 3-yl)methanol (542.73mg, 2.64mmol) and 4A molecular sieve powder (0.3g) in a slurry of a mixture of dry 1,2-dichloroethane (35mL) and acetonitrile (20mL), stirred at room temperature. After the reaction, the reaction solution was vacuum filtered and the solvent was removed in vacuum. The residue was dissolved in 60 ml of ethyl acetate, washed with water and saturated sodium chloride solution respectively, the organic layer was dried with anhydrous sodium sulfate, filtered, and the filtrate was concentrated under reduced pressure. The residue was separated and purified by silica gel column chromatography (dichloromethane:methanol=50:1) to obtain 3-(6,7-dimethoxy-1-(4-methoxybenzyl))- 3,4-Dihydroisoquinoline-2(1H)-yl)propyl((1-phenethylpyrrolidin-3-yl)methyl)maleate 1.23g, the yield was 73.76%.

¹ H NMR(300MHz,DMSO-d6)δ 7.37-7.16(m,5H), 7.16-7.09(m,2H), 6.81(d,J=8.6Hz,2H), 6.74(s,2H), 6.64( s,1H),6.49(s,1H),4.18-3.80(m,4H),3.72(d,J=3.3Hz,6H),3.62(s,3H),3.37(d,J=20.1Hz,1H ), 3.21 (d, J=13.2 Hz, 1H), 2.97-2.68 (m, 6H), 2.68-2.30 (m, 10H), 2.02-1.82 (m, 1H), 1.76-1.17 (m, 3H).

ESI-MS: m/z 657.4.

Step 3: 6,7-Dimethoxy-1-(4-methoxybenzyl)-2-methyl-2-(3-((4-((1-methyl-1-phenylethyl) (Pyrrolidine-1-onium-3-yl) methoxy)-4-side oxybut-2-enyl) oxy) propyl)-1,2,3,4-tetrahydroisoquinoline- Preparation of 2-Onium Iodide (Compound 11)

At room temperature, add 3-(6,7-dimethoxy-1-(4-methoxybenzyl))-3,4-dihydroisoquinolin-2(1H)-yl)propyl ( (1-Phenethylpyrrolidinbut-3-yl)methyl)maleate (150mg, 0.237mmol) was dissolved in 10ml of dry acetonitrile, methyl iodide (337.53mg, 2.37mmol) was slowly added, and the reaction was stirred. After the completion of the reaction, the solvent was removed under reduced pressure, ethyl acetate was added, sonicated for 5 minutes, filtered, the filter cake was washed with ethyl acetate, and dried under reduced pressure to obtain 123.60 mg of compound 11 with a yield of 78.65%.

¹ H NMR (300MHz, DMSO- d ₆ ) δ 7.45-7.21 (m, 6H), 6.98 (dd, J =17.0, 8.4 Hz, 2H), 6.91-6.76 (m, 4H), 6.71-6.64 (m, 1H),5.56(d, J =12.1Hz,1H),4.83-4.64(dd,1H),4.41-4.10(m,3H),3.81(s,2H),3.73(s,6H),3.65(m ,3H),3.56(m,2H),3.38(d, J =13.4Hz,4H),3.22(t, J =2.5Hz,3H),3.16(d, J =5.0Hz,3H),3.11(d , J =7.4Hz,4H), 3.00(d, J =10.1Hz,2H), 2.86(q, J =12.6Hz,2H), 2.37(s,2H),2.21(s,1H),1.95(s ,1H),1.24(s,1H).

ESI-MS: m/z 343.2.

Example 12

(1R,2S)-2-(3-((4-(3-(benzyldimethylammonium)propoxy)-4-oxobut-2-enyl)oxy)propyl) -6,7-Dimethoxy-1-(4-methoxybenzyl)-2-methyl-1,2,3,4-tetrahydroisoquinolin-2-onium hexafluorophosphate ( 1 -1 ) and (1R,2S)-2-(3-((4-(3-(benzyldimethylammonium)propoxy)-4-side oxybut-2-enyl)oxy )Propyl)-6,7-dimethoxy-1-(4-methoxybenzyl)-2-methyl-1,2,3,4-tetrahydroisoquinolin-2-ium chloride ( 1-2 ) Preparation

Step 1: Synthesis of 3-(benzyldimethylammonium) propyl sulfate (compound 1-1-01)

N,N-Dimethylbenzylamine (1-1-B) (10.00g, 73.96mmol) was dissolved in 100ml of acetonitrile, propylene sulfate (20.43g, 147.92mmol) was added, and the temperature was raised to reflux for reaction. After the reaction was completed, it was filtered, the filter cake was washed with a small amount of acetonitrile, and the filter cake was vacuum dried to obtain 19.40 g of compound 1-1-01 with a yield of 95.94%.

Step 2: Synthesis of N-benzyl-3-hydroxy-N,N-dimethylpropane-1-ammonium chloride (compound 1-1-02)

Compound 1-1-01 (18.00 g, 65.85 mmol) was added to 180 ml of methanol, sulfuric acid (0.35 ml, 6.59 mmol) was added, and the temperature was raised to reflux for reaction. After the reaction is completed, it is naturally cooled to room temperature, and 90 ml of activated chloride ion type anion exchange resin LX-300C is used to pack the column, and the column is repeatedly eluted with methanol 4 times. The extract was concentrated under reduced pressure to obtain 12.53 g of compound 1-1-02 with a yield of 82.8%.

Step 3: Synthesis of N-benzyl-3-((3-methanopropenyl)oxy)-N,N-dimethylpropane-1-ammonium chloride (Compound 1-1-03)

Compound 1-1-02 (11.60 g, 50.49 mmol) was added to 110 ml of acetonitrile, maleic anhydride (5.88 g, 60 mmol) and triethylamine (5.57 g, 55 mmol) were added, and the reaction was stirred at room temperature. After the reaction is complete, add 5% hydrochloric acid to adjust the pH to 3, concentrate under reduced pressure and evaporate to dryness. The residue obtained is separated and purified by column chromatography (extractant V _DCM : V _MeOH = 50:1~5:1) to obtain 11.79g of compound 1-1-03, the yield was 71.24%.

¹ H NMR(400MHz,DMSO- d ₆ )δ 7.60(d,J=6.8Hz,2H),7.55-7.48(m,3H),6.41(d,J=12.0Hz,1H),5.93(d,J =12.0Hz, 1H), 4.59 (s, 2H), 4.15-4.13 (m, 2H), 3.51-3.47 (m, 2H), 3.00-2.97 (m, 6H), 2.16 (s, 2H).

Step 4: (1R,2S)-2-(3-((4-(3-(benzyldimethylammonium)propoxy)-4-oxobut-2-enyl)oxy) Propyl)-6,7-dimethoxy-1-(4-methoxybenzyl)-2-methyl-1,2,3,4-tetrahydroisoquinolin-2-onium hexafluorophosphate Synthesis of salt ( 1-1)

Compound 1-1-03 (1.43g, 4.35mmol) was added to 20ml of dichloromethane, HATU (1.83g, 4.80mmol) and DIPEA (0.62g, 4.80mmol) were added with stirring, and (1R, 2S) was added- 2-(3-hydroxypropyl)-6,7-dimethoxy-1-(4-methoxybenzyl)-2-methyl-1,2,3,4-tetrahydroisoquinoline- 2-Onium chloride (Compound 1-1-A, prepared according to the method disclosed in WO2014005122) (1.84 g, 4.35 mmol), the reaction was stirred at room temperature. After the reaction is complete, wash with saturated sodium carbonate solution, water and saturated brine, separate the organic layer, dry with anhydrous sodium sulfate, filter, and concentrate the filtrate under reduced pressure. The residue was subjected to column chromatography (extractant V _DCM : V _MeOH = 80:1-10:1) to obtain 2.75 g of compound 1-1 (hexafluorophosphate salt) with a yield of 66.43%.

¹ H NMR (400MHz, DMSO- d ₆ ) δ 7.59-7.52 (m, 5H), 7.01-6.94 (m, 2H), 6.88-6.83 (m, 5H), 5.59 (s, 1H), 4.77 (d, J=7.6Hz, 1H), 4.59-4.57 (m, 2H), 4.39-4.36 (m, 1H), 4.28-4.25 (m, 2H), 3.90-3.81 (m, 1H), 3.78- 3.72 (m, 8H), 3.55-3.52(m, 1H), 3.45-3.41(m, 2H), 3.37-3.31(m, 4H), 3.22(s, 3H), 3.16-3.12(m, 2H), 3.02-3.00( m, 7H), 2.89-2.82 (m, 1H), 2.39-2.33 (m, 2H), 2.25-2.21 (m, 2H).

_{ESI-MS: [C 39 H} 52 F 12 N 2 O 7 P 2 -2PF 6 -] 2+ m / z 330.39.

Step 5: (1R,2S)-2-(3-((4-(3-(benzyldimethylammonium)propoxy)-4-oxobut-2-enyl)oxy) Propyl)-6,7-dimethoxy-1-(4-methoxybenzyl)-2-methyl-1,2,3,4-tetrahydroisoquinolin-2-onium chloride ( 1-2 ) Synthesis

Compound 1-1-03 (5.72g, 17.40mmol) was added to a mixed solvent of 25ml of dichloromethane and 25ml of acetonitrile, oxalic chloride (11.04g, 87.00mmol) was added with stirring, the reaction was carried out at room temperature, and then concentrated under reduced pressure to remove Solvent and urethane chloride. Then add 25ml of dichloromethane and 25ml of acetonitrile to reconstitute it, then add 30ml of a dichloromethane solution of compound 1-1-1 (7.36g, 17.40mmol), and stir to react at room temperature. After the completion of the reaction, it was concentrated under reduced pressure, and the residue was separated and purified by column chromatography (extractant V _DCM : V _MeOH =80:1~10:1, adding 1‰ concentrated hydrochloric acid) to obtain 7.21g of compound 1-2 (Chloride ion salt), the yield is 56.64%.

¹ H NMR (400MHz, DMSO- d ₆ ) δ 7.66-7.64 (m, 2H), 7.54-7.48 (m, 3H), 7.10-7.07 (m, 2H), 6.83-6.82 (m, 3H), 6.75 ( s, 2H), 5.54 (s, 1H), 4.88-4.85 (m, 1H), 4.77 (s, 2H), 4.26 (t, J=5.6, 2H), 4.21-4.14 (m, 2H), 3.97- 3.94 (m, 1H), 3.86-3.78 (m, 1H), 3.72-3.64 (m, 7H), 3.59-3.46 (m, 4H), 3.46 (s, 3H), 3.20 (s, 3H), 3.11 3.08 (m, 8H), 2.89 (t, J=11.6, 1H), 2.25 (s, 4H).

ESI-MS: m/z [C ₃₉ H ₅₂ Cl ₂ N ₂ O ₇ -2Cl] ²⁺ 330.39.

Test example 1: Pharmacodynamic test

1. Test drug

Sample: Compounds 1 to 10 of the present disclosure; CW1759-50 (specific configuration isomer described in the patent) and its corresponding racemate (prepared according to the method described in WO2014005122) described in WO2014005122, all using physiological saline Configuration.

CW1759-50

2. Experimental animals

SD rats (source: Changsha Tianqin Biotechnology Co., Ltd.), male, weighing 300g-350g before the test; rearing environment: SPF level.

3. Experimental steps

1) The rat is weighed and anesthetized by intraperitoneal injection with 25% urethane at 10 mL/kg, and then fixed on the rat operating bed supine, separating the trachea and external jugular vein, inserting the tracheal intubation and venous intubation, respectively, prepare Good respirator. After a few minutes, the righting reflex disappeared for the next experiment.

2) Sciatic nerve-skeletal muscle connection instrument: After the sciatic nerve and skeletal muscle are separated by operation, the skeletal muscle is connected to the tension transducer, and the sciatic nerve is placed with stimulation electrodes. After the connection is completed, the rat is stable for 10 min.

3) Give TOF (four series of stimuli) to stimulate the sciatic nerve to induce muscle contraction of skeletal muscle. After a period of stability, record a normal muscle contraction curve.

4) After the signal is stable, different doses of the test compound are injected through the jugular vein, and the inhibition of muscle tension after the administration is recorded.

Test index:

1) Compare the neuromuscular blockade efficacy of each compound, namely ED95 (drug dose that can cause 95% neuromuscular blockade), onset time, recovery time, etc.

2) Compare the reversal effects of intravenous injection of the same dose (200mg/kg) of L-cysteine on the above compounds.

4. Results

The ED95, onset and duration of the muscle relaxation effect of the compounds of the present disclosure on rats, and the reversal of the drug muscle relaxation effect of L-cysteine are shown in Table 1 and Table 2 below.

Table 1

Table 2

Compared with CW1759-50, the compounds described in the present disclosure exhibit excellent neuromuscular blockade efficacy and have the advantage of shorter acting. In addition, the compound of the present disclosure and CW1759-50 can both be inactivated by L-cysteine and produce the effect of reversing muscle relaxation. The compound of the present disclosure can be quickly reversed by L-cysteine (<1min ), and the reversal speed is faster than CW1759-50 (>3min).

Claims (25)

A compound represented by formula I or a pharmaceutically acceptable salt thereof or their stereoisomers, rotamers or tautomers,

in, R ₁ and R ₂ are each independently selected from hydrogen atoms and halogens; T is selected from -CH ₂ -and -CH ₃ , wherein if T is -CH ₃ , there is no phenyl group _{with Y 1} to Y _{5 substituents;} W is selected from -CH ₃ and

, Wherein Z ₁ , Z ₂ , Z ₃ , Z ₄ and Z ₅ are each independently selected from hydrogen, hydroxyl and methoxy, or any two adjacent Z ₁ , Z ₂ , Z ₃ , Z ₄ or Z ₅ Together to form methylenedioxy or ethylenedioxy; X ₁ , X ₂ , X ₃ and X ₄ are each independently selected from hydrogen, hydroxyl and methoxy, or any two adjacent X ₁ , X ₂ , X ₃ and X ₄ together form a methylene dioxy group or Ethylenedioxy; Y ₁ , Y ₂ , Y ₃ , Y ₄ and Y ₅ are each independently selected from hydrogen, hydroxyl and methoxy, or any two adjacent Y ₁ , Y ₂ , Y ₃ , Y ₄ or Y ₅ together form methylenedioxy or ethylenedioxy; A is selected from

with

, in, R ₃ and R ₄ are each independently selected from alkyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, halogen, hydroxy, mercapto, alkoxy and alkylthio, wherein the alkyl, cycloalkyl , Heterocyclyl, aryl or heteroaryl optionally further selected from alkyl, halogen, hydroxyl, mercapto, amino, pendant oxy, carboxy, nitro, cyano, alkoxy, alkylthio, ring Substituted by one or more substituents in the alkyl group, heterocyclic group, aryl group and heteroaryl group; R ₅ and R ₆ are each independently selected from a hydrogen atom, an alkyl group, a halogen, a hydroxyl group, a mercapto group, an amino group, a carboxyl group, a nitro group, a cyano group, an alkoxy group and an alkylthio group; Ring G _{1 is} selected from aryl and heteroaryl; R _{7 is} selected from alkyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, halogen, hydroxy, mercapto, alkoxy and alkylthio, wherein the alkyl, cycloalkyl, heterocyclyl, aryl The group or heteroaryl group is optionally further selected from alkyl, halogen, hydroxyl, mercapto, amino, pendant oxy, carboxy, nitro, cyano, alkoxy, alkylthio, cycloalkyl, heterocyclic group , Substituted by one or more substituents in the aryl and heteroaryl groups; R ₉ and R ₁₀ are each independently selected from a hydrogen atom, an alkyl group, a halogen, a hydroxyl group, a mercapto group, an amino group, a carboxyl group, a nitro group, a cyano group, an alkoxy group and an alkylthio group; Ring G _{2 is} each independently selected from aryl and heteroaryl; Ring B is a 4-8 membered heterocyclic group; R _{8 is} selected from alkyl, halogen, hydroxyl, mercapto, amine, carboxy, nitro, cyano, alkoxy and alkylthio; Each R _{11 is} independently selected from alkyl, halogen, hydroxyl, mercapto, amine, carboxy, nitro, cyano, alkoxy and alkylthio; or at least one R ₁₁ and ring G ₁ together form ring M _1. The ring M ₁ is a fused ring aryl group or a fused heteroaryl group, wherein the ring M _{1 is} optionally further selected from alkyl, halogen, hydroxyl, mercapto, amine, pendant oxy, carboxy, nitro, cyano Substituted by one or more substituents in the group, alkoxy group, alkylthio group, cycloalkyl group, heterocyclic group, aryl group and heteroaryl group; Each R _{12 is} independently selected from alkyl, halogen, hydroxyl, mercapto, amine, carboxy, nitro, cyano, alkoxy and alkylthio; or at least one R ₁₂ and ring G ₂ together form ring M _2. The ring M ₂ is a fused ring aryl group or a fused heteroaryl group, wherein the ring M _{2 is} optionally further selected from alkyl, halogen, hydroxyl, mercapto, amine, pendant oxy, carboxy, nitro, cyano Substituted by one or more substituents in the group, alkoxy group, alkylthio group, cycloalkyl group, heterocyclic group, aryl group and heteroaryl group; m is selected from 0, 1, 2 and 3; n is selected from 1, 2, 3, 4, 5 and 6; p is selected from 0, 1, 2, 3, 4, 5, 6, 7 and 8; q ₁ and q ₂ are each independently selected from 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12; x ₁ and x ₂ are each independently selected from 0, 1, 2, 3, 4, and 5. The compound according to claim 1, or a pharmaceutically acceptable salt thereof, or their stereoisomers, rotamers or tautomers, wherein R ₃ and R ₄ are both C ₁ -C ₆ alkyl groups, wherein Alkyl is optionally further selected from alkyl, halogen, hydroxyl, mercapto, amine, pendant oxy, carboxy, nitro, cyano, alkoxy, alkylthio, cycloalkyl, heterocyclic, aryl And one or more substituents in the heteroaryl group. The compound according to claim 1 or 2, or a pharmaceutically acceptable salt thereof, or a stereoisomer, rotamer, or tautomer thereof, wherein R ₅ and R ₆ are each independently selected from a hydrogen atom, C ₁ -C ₆ alkyl, halogen, hydroxyl and C ₁ -C ₆ alkoxy, preferably all hydrogen atoms; q _{1 is} selected from 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and 12, preferably 0 or 1. The compound according to claim 1, or a pharmaceutically acceptable salt thereof, or their stereoisomers, rotamers or tautomers, wherein each R _{11 is} independently selected from the group consisting of alkyl, halogen, hydroxy, and mercapto , Amine group, carboxyl group, nitro group, cyano group, alkoxy group and alkylthio group; or any two adjacent R ₁₁ and ring G ₁ together form a ring M ₁ , the ring M ₁ is a fused ring aryl group or a fused ring Heteroaryl, wherein the ring M _{1 is} optionally further selected from alkyl, halogen, hydroxy, mercapto, amine, pendant oxy, carboxy, nitro, cyano, alkoxy, alkylthio, cycloalkyl , Substituted by one or more substituents of heterocyclic group, aryl group and heteroaryl group; Each R _{12 is} independently selected from alkyl, halogen, hydroxyl, mercapto, amine, carboxy, nitro, cyano, alkoxy and alkylthio; or any two adjacent R ₁₂ and ring G ₂ Together to form a ring M ₂ , the ring M ₂ is a fused ring aryl group or a fused heteroaryl group, wherein the ring M _{2 is} optionally further selected from alkyl, halogen, hydroxyl, mercapto, amine, pendant oxy, carboxy, One or more substituents among nitro, cyano, alkoxy, alkylthio, cycloalkyl, heterocyclic, aryl and heteroaryl groups are substituted. The compound according to any one of claims 1 to 4, or a pharmaceutically acceptable salt thereof, or a stereoisomer, rotamer, or tautomer thereof, wherein each R _{11 is} independently selected from C ₁ -C ₆ alkyl, halogen, hydroxy, nitro, cyano and C ₁ -C ₆ alkoxy; or any two adjacent R ₁₁ and ring G ₁ together form a ring M ₁ , wherein the ring M _{1 is} any Optionally further selected from alkyl, halogen, hydroxyl, mercapto, amine, pendant oxy, carboxy, nitro, cyano, alkoxy, alkylthio, cycloalkyl, heterocyclic, aryl and heteroaryl One or more substituents in the group are substituted. The compound according to any one of claims 1 to 5, or a pharmaceutically acceptable salt thereof, or their stereoisomers, rotamers or tautomers, wherein the ring M _{1 is} selected from:

The compound according to any one of claims 1 to 6, or a pharmaceutically acceptable salt thereof, or a stereoisomer, rotamer, or tautomer thereof, wherein ring G ₁ is a phenyl group. The compound according to claim 7, or a pharmaceutically acceptable salt thereof, or a stereoisomer, rotamer or tautomer thereof, wherein the ring M _{1 is} selected from:

The compound according to claim 1, or a pharmaceutically acceptable salt thereof, or a stereoisomer, rotamer or tautomer, wherein R ₇ is a C ₁ -C ₆ alkyl group, wherein the alkyl group optionally Further selected from alkyl, halogen, hydroxyl, mercapto, amine, pendant oxy, carboxy, nitro, cyano, alkoxy, alkylthio, cycloalkyl, heterocyclic, aryl and heteroaryl Is substituted by one or more substituents. The compound according to claim 1 or 9, or a pharmaceutically acceptable salt thereof, or a stereoisomer, rotamer, or tautomer thereof, wherein R ₉ and R ₁₀ are each independently selected from a hydrogen atom, C ₁ -C ₆ alkyl, halogen, hydroxy and C ₁ -C ₆ alkoxy, preferably all hydrogen atoms; q _{2 is} selected from 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and 12, preferably 0, 1 or 2. The compound according to any one of claims 1 or 9 to 10, or a pharmaceutically acceptable salt thereof, or a stereoisomer, rotamer, or tautomer thereof, wherein each R _{12 is} independently selected from C ₁ -C ₆ alkyl, halogen, hydroxy, nitro, cyano and C ₁ -C ₆ alkoxy; or any two adjacent R ₁₂ and ring G ₂ together form a ring M ₂ , wherein the ring M _{2 is} optionally further selected from alkyl, halogen, hydroxyl, mercapto, amine, pendant oxy, carboxy, nitro, cyano, alkoxy, alkylthio, cycloalkyl, heterocyclic, aryl and One or more substituents in the heteroaryl group are substituted. The compound according to any one of claims 1 or 9 to 11, or a pharmaceutically acceptable salt thereof, or their stereoisomers, rotamers or tautomers, wherein ring M _{2 is} selected from:

The compound as described in any one of claims 1 or 9 to 12, or a pharmaceutically acceptable salt thereof, or a stereoisomer, rotamer, or tautomer thereof, wherein ring G ₂ is a phenyl group. The compound according to claim 13, or a pharmaceutically acceptable salt thereof, or their stereoisomers, rotamers or tautomers, wherein the ring M _{2 is} selected from:

The compound according to any one of claims 1 or 9 to 14, or a pharmaceutically acceptable salt thereof, or their stereoisomers, rotamers or tautomers, wherein ring B is selected from:

The compound according to any one of claims 1 to 15, or a pharmaceutically acceptable salt thereof, or their stereoisomers, rotamers or tautomers, wherein the compound represented by formula I is selected From the compound represented by formula Ia or its pharmaceutically acceptable salt or their stereoisomers, rotamers or tautomers,

Wherein, Q is a pharmaceutically acceptable anion, and preferably Q is each independently selected from halogen, acetate, benzoate, benzenesulfonate, camphorsulfonate, citrate, ethanedisulfonate, fumarate, and glucone Sugar, gluconate, glucuronate, isethionate, lactate, lacturonate, lauryl sulfate, malate, maleate, methanesulfonate, naphthoate, naphthalenesulfonate, nitric acid Root, stearate, oleate, oxalate, pamoate, phosphate, hydrogen phosphate, dihydrogen phosphate, polygalacturonate, succinate, sulfate, sulfosalicylate, tartrate, Tosylate, trifluoroacetate; y is 0.1 to 4; z is selected from 1, 2, 3, 4; Among them, X ₁ -X ₄ , Y ₁ -Y ₅ , W, T, R ₁ , R ₂ , A, m, and n are as defined in claim 1. A compound represented by formula II or a pharmaceutically acceptable salt thereof or their stereoisomers, rotamers or tautomers,

Wherein, A and n are as defined in claim 1; Y ₁ , Y ₂ , Y ₃ , Y ₄ and Y ₅ are each independently selected from a hydrogen atom, a hydroxyl group and a methoxy group. The compound according to claim 17, or a pharmaceutically acceptable salt thereof, or their stereoisomers, rotamers or tautomers, wherein _{1 of} Y 1, Y ₂ , Y ₃ , Y ₄ and Y ₅ One is a methoxy group, and the remaining 4 are hydrogen atoms; or _{2 of Y 1} , Y 2, Y ₃ , Y ₄ and Y ₅ are methoxy groups, and the remaining 3 are hydrogen atoms; or Y ₁ , Y _{2 3 of} , Y 3, Y ₄ and Y ₅ are methoxy groups, and the remaining 2 are hydrogen atoms; or 4 of Y ₁ , Y ₂ , Y ₃ , Y ₄ and Y ₅ are methoxy groups, and the remaining One is a hydrogen atom; or Y ₁ , Y ₂ , Y ₃ , Y ₄ and Y ₅ are all methoxy groups; or Y ₁ , Y ₂ , Y ₃ , Y ₄ and Y ₅ are all hydrogen atoms. The compound according to claim 17 or 18, or a pharmaceutically acceptable salt thereof, or their stereoisomers, rotamers or tautomers, wherein the compound represented by formula II is selected from the compound represented by formula IIa or Pharmaceutically acceptable salts or their stereoisomers, rotamers or tautomers,

Wherein, Q is a pharmaceutically acceptable anion, and preferably Q is each independently selected from halogen, acetate, benzoate, benzenesulfonate, camphorsulfonate, citrate, ethanedisulfonate, fumarate, and meglucone Sugar, gluconate, glucuronate, isethionate, lactate, lacturonate, lauryl sulfate, malate, maleate, methanesulfonate, naphthoate, naphthalenesulfonate, nitric acid Root, stearate, oleate, oxalate, pamoate, phosphate, hydrogen phosphate, dihydrogen phosphate, polygalacturonate, succinate, sulfate, sulfosalicylate, tartrate, Tosylate, trifluoroacetate; y is 0.1 to 4; z is selected from 1, 2, 3, 4; Among them, Y ₁ -Y ₅ , A, and n are as defined in claim 16. The compound according to claim 1, or a pharmaceutically acceptable salt thereof, or their stereoisomers, rotamers or tautomers, wherein the compound is selected from:

The compound according to claim 1, or a pharmaceutically acceptable salt thereof, or their stereoisomers, rotamers or tautomers, wherein the compound is selected from:

The compound according to any one of claims 1 to 21, or a pharmaceutically acceptable salt thereof, or their stereoisomers, rotamers or tautomers, wherein one or more hydrogens in the compound are deuterated replace. A method for preparing the compound according to any one of claims 1 to 22, or a pharmaceutically acceptable salt thereof, or their stereoisomers, rotamers or tautomers, which comprises combining a compound of formula III with formula IV compound mixing reaction steps,

Among them, _{the definitions of X 1} -X ₄ , Y ₁ -Y ₅ , W, T, R ₁ , R ₂ , A, m, and n are as described in claim 1; R _{a is} selected from hydroxyl and halogen; R _{b is} selected from hydroxyl. A pharmaceutical composition comprising the compound according to any one of claims 1 to 22, or a pharmaceutically acceptable salt thereof, or their stereoisomers, rotamers, or tautomers, and pharmaceutically acceptable The carrier, diluent or excipient. A compound as described in any one of claims 1 to 22 or a pharmaceutically acceptable salt thereof or their stereoisomers, rotamers or tautomers or the pharmaceutical composition as described in claim 22 Use in preparing medicine for neuromuscular blockade.