TW202045786A - Fiber with cell affinity and fiber product made using the same - Google Patents

Abstract

A fiber with cell affinity and a fiber product made using the same are provided. The fiber with cell affinity is a collagen surface-treated polyethylene terephthalate fiber having a profiled cross section.

Description

Fiber with cell affinity and fiber products made from it

The present invention relates to a fiber and a fiber product made from the fiber, and particularly to a fiber with cell affinity and a fiber product made from the fiber.

In view of the rupture and reconstruction of tendons or ligaments, current clinical operations mainly focus on autogenous tendons or ligaments with soft tissue fixation devices. The implants of tendons or ligaments need to be strengthened to overcome clinical problems. For example, repairing with autologous tissue has a certain impact on the patient's transplantation site. Artificial ligaments cannot be implanted for a long time, and they are easy to wear and loosen. In addition, existing products also have shortcomings of insufficient biocompatibility, such as poor hydrophilicity on the surface of PET and unfavorable cell attachment. On the other hand, product technology must rely on imports, which in turn leads to the problem of high costs.

Based on the above, the development of a fiber that can increase the surface area of the fiber and has cell affinity, and is used to manufacture fiber products such as artificial ligaments, is an important subject for current research.

The present invention provides a fiber with cell affinity and a fiber product made from the fiber, which can increase the surface area of the fiber, improve the adhesion of cells and materials, and improve the compatibility of cell tissues.

The cell-affinity fiber of the present invention is a polyethylene terephthalate fiber surface-treated with collagen and has a special-shaped cross section.

In an embodiment of the present invention, relative to the polyethylene terephthalate fiber, the content of collagen is 1 wt% to 4 wt%.

In an embodiment of the present invention, the special-shaped section includes a cross section, a straight section, a wave section, a star section or a triangular section.

In an embodiment of the present invention, compared to the circular cross section, the cross section increases the fiber surface area of the fiber with cell affinity by 20% to 30%, and the straight cross section increases the fiber surface area of the fiber with cell affinity. The fiber surface area increases by 10% to 20%.

In one embodiment of the present invention, the cross section increases the cell proliferation effect of the fiber with cell affinity by 250% to 300% compared to the circular section, and the straight section increases the cell proliferation effect of the fiber with cell affinity. The cell proliferation effect is increased by 250% to 300%.

In an embodiment of the present invention, the surface treatment of collagen of polyethylene terephthalate fibers includes: dissolving collagen in acetic acid to make the pH between 2.5 and 3.5, and then adding PBS to simulate the growth environment. Adjust the pH value to 7 to 8 with NaOH solution to make the polyethylene terephthalate fiber react with the collagen.

In an embodiment of the present invention, the solid content of collagen in acetic acid is 0.01 wt% to 0.1 wt%.

In an embodiment of the present invention, the reaction time between polyethylene terephthalate fiber and collagen is 12 hours to 48 hours.

The fiber product of the present invention is made by using the above-mentioned cell-affinity fiber, and the fiber product includes an artificial ligament.

In an embodiment of the present invention, the weaving method of the artificial ligament may include planar weaving or three-dimensional weaving, and the strength of the artificial ligament is 1500N to 3000N.

Based on the above, the present invention provides a fiber with cell affinity, which is a polyethylene terephthalate fiber treated with collagen and has a special-shaped cross section. In this way, the surface area of the fiber can be increased, the adhesion of cells and/or materials can be improved, and the cell tissue compatibility can be improved. On the other hand, the present invention also provides a fiber product, which is made by using the above-mentioned cell-affinity fiber, and the fiber product includes an artificial ligament.

In order to make the above-mentioned features and advantages of the present invention more obvious and understandable, the following embodiments are specially cited and described in detail as follows.

In this article, the range represented by "a value to another value" is a summary expression method that avoids listing all the values in the range one by one in the specification. Therefore, the record of a specific numerical range covers any numerical value in the numerical range and the smaller numerical range defined by any numerical value in the numerical range, as if the arbitrary numerical value and the numerical value are clearly written in the specification. The smaller numerical range is the same.

The invention provides a fiber with cell affinity, which is a polyethylene terephthalate fiber treated with collagen and has a special-shaped section. In more detail, the special-shaped section may include a cross section, a straight section, a wavy section, a star-shaped section or a triangular section, but the present invention is not limited thereto. Compared with the circular cross section, the cross section can increase the fiber surface area of the fiber with cell affinity by, for example, 20% to 30%, and the straight cross section can increase the fiber surface area of the fiber with cell affinity by, for example, 10%. To 20%. Compared with the circular section, the cross section can increase the cell proliferation effect of fiber with cell affinity, for example, 250% to 300%, and the straight section can increase the cell proliferation effect of fiber with cell affinity, for example, 250 % To 300%. The fiber diameter of the circular section is, for example, 15 μm to 20 μm, the fiber diameter of the cross section is, for example, 15 μm to 25 μm, and the fiber diameter of the straight section is, for example, 20 μm to 30 μm. It should be particularly noted that the above-mentioned in-line cross-section fiber will be affected by processing conditions during its molding process and have a crimped or wavy elongated appearance, rather than a rectangular parallelepiped structure with a smooth surface.

In this embodiment, the content of collagen relative to the polyethylene terephthalate fiber is, for example, 1 wt% to 4 wt%. In more detail, the collagen surface treatment of polyethylene terephthalate fibers may include the following steps. First, the collagen is dissolved in acetic acid so that the pH of the solution is, for example, 2.5 to 3.5, and the solid content of the collagen in the acetic acid is, for example, 0.01 wt% to 0.1 wt%. After that, PBS is added to simulate the growth environment, and the pH value is adjusted to, for example, 7 to 8 with NaOH solution to make the polyethylene terephthalate fiber react with the collagen. For example, the reaction time between polyethylene terephthalate fiber and collagen is, for example, 12 hours to 48 hours, for example, the reaction time is 36 hours at a temperature of 36.5°C.

The present invention also provides a fiber product, which is made by using the above-mentioned cell-affinity fiber, and the fiber product may include an artificial ligament. The weaving method of the artificial ligament may include flat knitting or three-dimensional knitting, and the strength of the artificial ligament is, for example, 1500 N to 3000 N, so as to meet the standard for the use of artificial ligaments.

Hereinafter, experimental examples are used to describe in detail the cell-affinity fibers of the above-mentioned embodiments and the fiber products made using them. However, the following experimental examples are not intended to limit the present invention. Experimental example

In order to prove that the fiber with cell affinity proposed by the present invention and the fiber products made by using it can increase the surface area of the fiber, improve the adhesion of cells and/or materials, and improve the compatibility of cell tissues, the following experiment is specially made. .

It must be noted that since the preparation method of the cell-affinity fiber has been described in detail above, the preparation of the cell-affinity fiber is omitted below for the convenience of description. Prepare fiber

The polyethylene terephthalate fibers with circular, cross, and in-line sections were surface-treated with collagen, and the reaction concentration of collagen and the collagen content are shown in Table 1 below. Table 1 Collagen reaction concentration (wt%) Collagen content (wt%) Round section PET (Comparative Example 1) 0.05% 1.8 Round section PET (Comparative Example 2) 0.025% 1.3 Round section PET (Comparative Example 3) 0.01% 0.8 Cross-section PET (Example 1) 0.05% 3.5 One-section PET (Example 2) 0.05% 3.6 Cross section PET (Example 3) 0.025% 3.4 One-section PET (Example 4) 0.025% 3.1 Cross section PET (Example 5) 0.01% 0.8 One-section PET (Example 6) 0.01% 1.1 Cell attachment performance test

The cells used are iPS, and the test conditions are as follows: (1) Fix the sample in a cell culture dish, take 100λ, a cell suspension with a concentration of 1×10 ⁵ cells/mL, and add it to a 96-well dish with a concentration of 5 mol% Incubate in CO ₂ at 37°C for 24 hours. (2) After taking out the sample, add the test substance prepared with 100λ to extract the cells in the sample, and then incubate for 24 hours at 37°C containing 5 mol% CO ₂ . (3) Replace the liquid with 100λ MTT solution (1mg/mL MTT dissolved in serum-free cell culture medium), and incubate for 1 hour at 37°C containing 5 mol% CO ₂ . (4) The enzyme immunoassay instrument uses a wavelength of 540nm for analysis and analyzes the original data.

The cell attachment performance test was performed on the fibers in Table 1, and the cell proliferation rate obtained by the test is listed in Table 2 below. It can be seen from Table 2 below that comparing the cell adhesion effect of the fiber itself, the proliferation rate of PET fiber in the cross section is 50% higher than that of the round section. After the surface treatment of collagen, the cell proliferation rate is over 250% growth. Table 2 Cell proliferation rate (%) LIGASTIC (Comparative Example 4) 123 Round section PET (Comparative Example 5) 100 Round section PET+Collagen (Comparative Example 2) 250 Cross section PET (Example 7) 180 One-section PET (Example 8) 152 Cross section PET+collagen (Example 3) 286 One-line section PET+collagen (Example 4) 298

In summary, the present invention provides a cell-affinity fiber, which is a polyethylene terephthalate fiber treated with collagen and has a special-shaped cross section. In this way, it can increase the fiber surface area and roughness, improve cell and/or material adhesion, and improve cell tissue compatibility. Therefore, it can overcome the lack of biocompatibility, poor surface hydrophilicity and unfavorable cell adhesion of existing products. Disadvantages. On the other hand, the present invention also provides a fiber product, which is made by using the above-mentioned cell-affinity fiber, and the fiber product includes an artificial ligament.

Although the present invention has been disclosed in the above embodiments, it is not intended to limit the present invention. Anyone with ordinary knowledge in the technical field can make some changes and modifications without departing from the spirit and scope of the present invention. The scope of protection of the present invention shall be determined by the scope of the attached patent application.

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Claims (10)

A fiber with cell affinity, which is a polyethylene terephthalate fiber treated with collagen and has a special-shaped cross section. The cell-affinity fiber described in the first item of the scope of patent application, wherein the content of the collagen is 1 wt% to 4 wt% relative to the polyethylene terephthalate fiber. According to the first item of the scope of patent application, the fiber with cell affinity, wherein the special-shaped section includes a cross section, a straight section, a wave section, a star section or a triangular section. The cell-affinity fiber described in item 3 of the scope of the patent application, wherein the cross-section increases the fiber surface area of the cell-affinity fiber by 20% to 30% compared to a circular cross-section, In addition, the in-line section increases the fiber surface area of the cell-affinity fiber by 10% to 20%. The cell-affinity fiber described in item 3 of the scope of patent application, wherein the cross-section increases the cell proliferation effect of the cell-affinity fiber by 250% to 300% compared to a circular cross-section , The one-line section increases the cell proliferation effect of the fiber with cell affinity by 250% to 300%. The cell-affinity fiber described in item 1 of the scope of patent application, wherein the collagen surface treatment of the polyethylene terephthalate fiber includes: The collagen is dissolved in acetic acid so that the pH value is between 2.5 to 3.5, and then PBS is added to simulate the growth environment, and the pH value is adjusted to 7 to 8 with NaOH solution to make the polyethylene terephthalate fiber Reacts with the collagen. The cell-affinity fiber described in item 6 of the scope of patent application, wherein the solid content of the collagen in the acetic acid is 0.01 wt% to 0.1 wt%. The cell-affinity fiber described in item 6 of the scope of patent application, wherein the reaction time between the polyethylene terephthalate fiber and the collagen is 12 hours to 48 hours. A fiber product is made of the cell-affinity fiber described in any one of items 1 to 8 in the scope of the patent application, and the fiber product includes an artificial ligament. The fiber product according to item 9 of the scope of patent application, wherein the weaving method of the artificial ligament includes plane weaving or three-dimensional weaving, and the strength of the artificial ligament is 1500 N to 3000 N.