TW202023592A - Method for extracting phenolic acid substance from purple coneflower capable of highly efficiently extracting phenolic acid substance from purple coneflower in high purity - Google Patents

Abstract

The present invention provides a method for extracting phenolic acid substance from purple coneflower, which includes the following steps: (a) adding the purple coneflower sample into a first solvent, wherein the first solvent contains ethanol and has the environment pH value of 5.9-6.3, adding an absorption/decoloring agent and heating, then placing still after filtering to obtain a precipitate and a filtrate; and (2) placing the filtrate from step (1) into a simulated moving bed system for purification and separation, so as to separate cichoric acid in the filtrate from the extract and separate caftaric acid contained in the filtrate from the raffinate. Thus, the present invention may apply a highly efficient method to extract and obtain the phenolic acid substance of purple coneflower in high purity.

Description

Method for extracting echinacea phenolic acid

The present invention relates to an extraction method, especially a method for extracting phenolic acids from echinacea.

According to, medicinal plants have been studied for a long time, and the substances in them have been further extracted and refined to prepare or add various kinds of medicines, skin care products or health foods. There are many recommended health foods that can improve colds and boost immunity, and echinacea is one of the popular plants. Echinacea (Purple Coneflower, Echinacea ) is a perennial herb of the Asteraceae family, which is rich in a variety of phenolic acids, polysaccharides, alkanamides and glycoproteins. Among them, the phenolic acid substances are mainly caffeic acid derivatives, including Cichoric acid, Caftaric acid, and Echinacea. Phenolic acids have multiple biological activities, such as preventing thrombosis, inhibiting tumor proliferation, and reducing the risk of cardiovascular disease and cancer. In particular, cichoric acid can be combined with influenza virus, thereby inhibiting the virus from entering the cell to multiply to prevent the virus from increasing. On the other hand, caffeoyl tartaric acid is a good antioxidant to reduce the formation of various diseases.

In the existing extraction technology, for the extraction of phenolic acids from echinacea, most of them are purely solvent extraction, and the elution is repeated multiple times with solvents of different concentrations. Or, the separation and extraction are performed by a single chromatography method, such as high performance liquid chromatography (High Performance Liquid Chromatography, HPLC). However, the current method has low production efficiency, not only low purity and long operation time, but also difficult to mass produce, resulting in increased costs.

In view of this, the team of the present invention feels that the current practice is not perfect and exhausts their minds and brains. Based on their years of experience in this research, they finally proposed a method for extracting echinacea phenolic acid in order to improve the above Lack of conventional technology.

In view of the above-mentioned problems, the purpose of the present invention is to provide a method for extracting echinacea to improve product purity and work efficiency.

In order to achieve the above objective, the present invention proposes a method for extracting echinacea phenolic acid substances. The steps include: (1) adding a sample of echinacea into a first solvent, the first solvent has ethanol and its environmental pH is 5.9 ~ 6.3, add an adsorption decolorizing agent and heat, filter and stand to obtain a precipitate and a filtrate; and (2) put the filtrate of step (1) into a simulated moving bed system for purification and separation, and the filtrate The cichoric acid is separated from the extract, and the caffeoyl tartaric acid in the filtrate is separated from the raffinate. In this way, it is possible to obtain high-purity echinacea with a high-efficiency method.

In addition, in step (2), the simulated moving bed system is divided into a first section, a second section and a third section, and the extraction liquid is composed of the first section and the second section The raffinate is obtained from the outlet between the third section and the second section. Thereby, the separation efficiency of cichoric acid and caffeoyl tartaric acid from the filtrate can be improved, and its purity can be improved.

Furthermore, in step (2), the stationary phase of the simulated moving bed system is a silicone gel with alkane of 8 to 30 carbons and a surface modified with multiple hydrophilic hydroxyl groups to provide compatibility with water phase substances and increase Its separation effect improves the purity of the product.

In this embodiment, in step (2), the mobile phase of the simulated moving bed system is a solvent mixed with 95% ethanol and 0.1% hydrochloric acid in a volume ratio of 30% to 70%. Thereby, the separation effect of cichoric acid and caffeoyl tartaric acid can be increased, and the content can be increased.

Preferably, in step (2), the mobile phase of the simulated moving bed system is a solvent mixed with 95% ethanol and 0.1% hydrochloric acid at a volume ratio of 35% to achieve the best separation performance.

In addition, in step (2), the flow rate of the discharge port between the first section and the second section is 2.8 mL/min, and the third section is relative to the outlet of the second section The flow rate is 2.4 mL/min, and the switching time of the simulated moving bed system is 2.7 minutes to 4.75 minutes. Thereby, the separation effect of cichoric acid and caffeoyl tartaric acid can be improved.

Preferably, in step (2), the switching time of the simulated moving bed system is 3.125 minutes to achieve the best separation performance.

In this embodiment, in step (1), 0.01% hydrochloric acid is used to adjust the environmental pH of the first solvent to 6.1 to increase the solubility of the echinacea sample, thereby increasing the content of phenolic acid in the filtrate .

Furthermore, in step (1), the adsorbent decolorizing agent is an activated carbon adsorbent to reduce impurities and pigments in the echinacea sample.

In summary, the method of echinacea phenolic acid substance proposed by the present invention is to add the echinacea sample into the first solvent with ethanol and adjust the pH of the environment to neutral and acidic, and add the adsorption Decoloring agent, filter the filtrate after standing still. Then, the filtrate is put into a simulated moving bed system for purification and separation to further obtain phenolic acids in the echinacea sample, including cichoric acid and caffeoyl tartaric acid. By this, the production efficiency can be improved, and high-purity phenolic acid can be obtained.

In order to enable your reviewer to clearly understand the content of the present invention, only the following text is used with illustrations.

The echinacea samples of the present invention are not limited to wild echinacea, artificially planted echinacea, dried echinacea, or thick echinacea provided by Taiwan Deruitech Co., Ltd. Extracts.

If the percentage concentration in the present invention is not specifically specified as a weight percentage concentration or a volume Mohr concentration, etc., it refers to a volume percentage concentration and is expressed as a percentage (%). Among them, it is prepared by dilute configuration with water (H ₂ O) as a solvent.

In the present invention, the verification of echinacea phenolic acid substance is measured by high performance liquid chromatography (High Performance Liquid Chromatography, HPLC), and the standard products are used to produce cichoric acid (Cichoric acid) and coffee acyl tartaric acid (Caftaric acid). acid) and echinacea (Echinacea) calibration curve for quantitative or qualitative phenolic acid substances. Among them, the chromatography column of high performance liquid chromatography uses Inertsil HPLC Columns, which is a high-purity spherical silica gel with OctaDecyDilance (ODS, C18) as the chemical bond. In addition, the mobile phase of high performance liquid chromatography is eluted with solvent A and solvent B with the gradient settings in Table 1, and the volume ratio of solvent A and solvent B is different to change its relative to cichoric acid and caffeoyl. The polarity of tartaric acid is used to separate the phenolic acid. Among them, the solvent A is 0.3% H ₃ PO ₄ , and the solvent B is pure acetonitrile (Acetonitrile, cyanomethane, chemical formula CH ₃ CN). The detection wavelength in this experiment is 330 nanometers (nm), the flow rate is 0.8 mL/min, and the temperature is 25∘C.

Table 1 Gradient design of high performance liquid chromatography (HPLC) analysis Time (min) Flow rate (mL/min) Solvent A volume ratio Solvent B volume ratio 0 0.8 87 13 10 0.8 87 13 25 0.8 75 25 30 0.8 75 25 45 0.8 60 40 60 0.8 20 80 65 0.8 0 0 70 0.8 0 0 80 0.8 87 13 90 0.8 87 13

In view of the lack of previous techniques, the inventors proposed the following experimental examples in order to obtain better extraction efficiency and product purity. Please refer to FIG. 1 and FIG. 2. This experimental example uses Supercritical Fluid Extraction (SFE) technology, and uses carbon dioxide as a solvent in the supercritical fluid (SCF) state to extract the phenolic acids in the echinacea sample, and proposes The two environmental parameters are the echinacea sample with auxiliary solvent and the control group without addition, and are represented by the experimental example and the control group in Fig. 1 and Fig. 2 respectively. Wherein, the aforementioned auxiliary solvent is ethanol (Ethanol, chemical formula CH ₃ CH ₂ OH).

As shown in Figure 1, it can be seen that the sample obtained from the control group without added auxiliary solvent is light brown, while the sample obtained by adding auxiliary solvent in this experimental example is dark green. Then, as shown in FIG. 2, the aforementioned samples were further qualitatively analyzed by high performance liquid chromatography (HPLC), which were sequentially divided into the standard group, the experimental example and the control group. Among them, the standard group is the spectrum obtained by experimenting with the aforementioned standard products, and three peaks are obtained, which are respectively Caftaric acid, Echinacea and Cichoric acid in sequence. As shown in the figure, compared with the standard group, it can be seen that the spectrum results of the experimental example and the control group do not show the aforementioned phenolic acid substances. Comparing the results of Fig. 1 and Fig. 2, it can be shown that the supercritical extraction method (SFE) can hardly extract the phenolic acid of echinacea, but can only remove part of the impurities in the echinacea sample, regardless of whether auxiliary solvents are added. .

Therefore, in order to solve the lack of effective extraction of phenolic acid, the inventor continued to conceive how to extract the phenolic acid of echinacea with a high-efficiency extraction method, and then proposed the extraction of echinacea phenolic acid disclosed in the present invention Material method. Please refer to FIG. 3, which is a block flow diagram of a preferred embodiment of the present invention. The present invention proposes a method for extracting echinacea phenolic acid. The steps include (1) (step S1) and (2) (step S2), which are decolorization, alcohol precipitation and simulated moving bed (Simulated Moving Bed, SMB), respectively system. In this way, high-efficiency methods can be used to extract high-purity echinacea.

The steps of the present invention for decolorization and alcohol precipitation are step (1). The detailed steps are as shown in the block flow diagram in Figure 4. Add the echinacea sample to a first solvent, and the first solvent is 60% ethanol (Step P1) to dissolve the echinacea sample. Then, adjust the environmental pH of the first solvent to 5.9 ~ 6.3. Preferably, 0.01% hydrochloric acid (hydrochloric acid, chemical formula HCl) is used to adjust the environmental pH of the first solvent to 6.1 (step P2) to increase the solubility of the echinacea sample, thereby increasing the phenolic acid content The content is dissolved in the above-mentioned first solvent.

Next, an adsorption decolorizing agent is added to the first solvent with the echinacea sample (step P3), heated with water, and stirring is continued during the process (step P4). Among them, the temperature of the water area pot is 75∘C and lasts for 30 minutes (step P5). In addition, after filtering while it is hot, the liquid is placed in a 4∘C environment (step P6), and a precipitate and a filtrate are obtained after at least 8 hours (step P7). The liquid after removing the precipitate is the filtrate, that is, the sample after decolorization and alcohol precipitation (step P8).

Furthermore, in the aforementioned step (1), the adsorption and decolorizing agent is an activated carbon adsorbent to reduce impurities and pigments in the echinacea sample, thereby relatively increasing the proportion of phenolic acid in the echinacea. Preferably, the activated carbon adsorbent used in this preferred embodiment is SS type activated carbon (Sewage Sludge-Based Activated Carbon), and in this experimental example, CS type activated carbon (Carbonized Sludge-Based Activated Carbon) is used. ). Among them, the SS type activated carbon has a larger specific surface area (m ² /g) than the CS type activated carbon, and has better adsorption performance.

And as shown in Figure 5, Figure 6 and Table 2, compare the effects of different types of activated carbon adsorbents on the phenolic acid in the filtrate. Among them, the control group, this experimental example and this embodiment shown in the figure are the adsorption decolorizer without the adsorption decolorizer, the adsorption decolorizer with C-S activated carbon, and the adsorption decolorizer with S-S activated carbon. And take the filtrate, compare its appearance and at the same time carry out quantitative analysis by high performance liquid chromatography (HPLC) to determine the content of phenolic acid. Among them, it can be seen from Fig. 5 that the color of the filtrate after treatment with SS type activated carbon is the lightest, indicating that its decolorization effect is better than treatment with CS type activated carbon, that is, it can remove most of the pigment components and enhance the echinacea The proportion of phenolic acids in flower samples. In addition, it can be seen from Figure 6 that all three treatment methods can obtain phenolic acid substances. At the same time, through quantitative analysis by high performance liquid chromatography, Table 2 shows that the filtrate treated with C-S activated carbon has the highest phenolic acid content, which is increased from 7.26% to 13.38%.

Table 2 Data of decolorization and alcohol precipitation samples Sampling volume (g) Weight after decolorization and alcohol precipitation (g) Volume after decolorization and alcohol precipitation (mL) Total substance concentration (ppm) Cichoric acid Caftaric acid Total phenolic acid weight content (wt%) concentration Weight content (wt%) concentration Weight content (wt%) Control group 1.00 0.48 100 4835 206.91 4.27 144.68 2.99 7.26 This experimental example (CS type activated carbon) 1.00 0.51 78 6,543.59 521.43 7.95 353.97 5.41 13.38 This example (SS type activated carbon) 1.00 0.45 74 6077.03 452.47 7.45 339.24 5.58 13.03

Following step (1), the filtrate after decolorization and alcohol precipitation is subjected to a simulated moving bed (SMB) system for purification and separation, that is, step (2). It is to put the filtrate obtained in step (1) into a simulated moving bed system, and the cichoric acid in the filtrate can be separated from the extract, and the caffeoyl tartaric acid in the filtrate can be separated (Caftaric acid) is separated from the raffinate. In this way, the high chromatographic resolution of the simulated moving bed can be used to obtain high-purity and high-content products.

Furthermore, because the echinacea sample has multiple components, the composition of the filtrate is complex. The configuration design of the simulated moving bed system is an open design with three sections, divided into a first section ( Section I), a second section (Section II) and a third section (Section III), as shown in the configuration design diagram of FIG. 7. Among them, the simulated moving bed system has two inlets and two outlets, namely a feed end (Feed), a mobile phase end (Desorbent), a discharge port (Extract to Recycle) and an outlet (Raffinate to Recycle). And the discharge port between the first section and the second section can obtain the raffinate, and the third section relative to the outlet of the second section can obtain the raffinate .

Continuing the above description, the optimized configuration design of the simulated moving bed system is obtained after many experiments. It is composed of eight chromatography columns (Columns), which are represented by C1 to C8. The number of chromatography columns in the section, the second section and the third section are two, three and three respectively. In addition, the stationary phase (Stationary Phase) of the simulated moving bed system is a silicone with 8-30 carbon alkanes and multiple hydrophilic hydroxyl groups modified on the surface. Preferably, this embodiment uses AQ-C18 (Aqueous ODS) chromatography column, which is bonded to the surface of silica gel with multiple hydrophilic hydroxyl groups, namely Si-O-Si-PG-C ₁₈ -H ₃₇ , Which is a stationary phase formed by Embeded Polar Group (EPG). Or in another embodiment, it is a stationary phase formed by hydrophilic endcapping after a plurality of alkanes are bonded to the surface of the silica gel and then treated with a hydrophilic group. In this way, it can provide a compatible environment for water phase substances, and make the filtrate have good retention and hydrophobicity, so as to enhance the separation effect of phenolic acid substances in it, and further increase the phenolic acid in the extract and raffinate. The purity of the substance.

On the other hand, the mobile phase of the simulated moving bed system is 95% ethanol (Ethanol, chemical formula CH ₃ CH ₂ OH) and 0.1% hydrochloric acid (hydrochloric acid, chemical formula HCl) in volume ratio 30% to 70% mixed solvent.

Preferably, in this embodiment, the mobile phase of the simulated moving bed system is a solvent mixed with 95% ethanol and 0.1% hydrochloric acid at a volume ratio of 35%. And use the aforementioned solvent as the mobile phase of the simulated moving bed system for qualitative analysis by high performance liquid chromatography (HPLC). As shown in Figure 8, it can be seen that the echinacea sample can effectively separate cichoric acid and caffeoyl tartaric acid. In this way, the phenolic acid substances can be effectively separated under this condition, that is, the cichoric acid and caffeoyl tartaric acid in the filtrate can achieve the best separation effect.

In other experimental examples, the mobile phase is made of 95% ethanol and is configured in a different volume ratio with 0.1% hydrochloric acid, including 25%, 35%, 45%, and 65%, which means 95% ethanol and 0.1% hydrochloric acid. The volume ratio of% hydrochloric acid is 25%: 75%, 35%: 65%, 45%: 55%, and 65%: 35%. And qualitative analysis by high-performance liquid chromatography (HPLC), the results of the spectrum are shown in Figure 9, respectively expressed as 25%EtOH-75%(0.01%HCl), 35%EtOH-65%(0.01%HCl) ), 45% EtOH-55% (0.01% HCl) and 65% EtOH-35% (0.01% HCl). As shown in the figure, there is a significant difference between 95% ethanol and 0.1% hydrochloric acid in different volume ratios as mobile phases. Among them, with the decrease of 95% ethanol, the resolution of phenolic acids increases. However, the residence time of the simulated moving bed system is also increased, and the production time is increased, and the desorption capacity of phenolic acid substances is also reduced, that is, the content of phenolic acid substances is reduced due to the decrease of the volume ratio of 95% ethanol . When the volume ratio of 95% ethanol to 0.1% hydrochloric acid is 65% and 45%, that is, 65% EtOH-35% (0.01% HCl) shown in the figure, it can be seen from the wave peak that the scattered light intensity is higher, that is Phenolic acids have high desorption capacity and can be completely desorbed within 8 minutes. However, it is a single peak wave type, which means that cichoric acid and caffeoyl tartaric acid cannot be effectively separated. Preferably, the 35% EtOH-65% (0.01%HCl) shown in the above-mentioned solvent is configured at a ratio of 35%, which can completely desorb the phenolic acid substance within 15 minutes, and at the same time It has good resolution, and can effectively separate cichoric acid and caffeoyl tartaric acid.

In addition, in the simulated moving bed system of step (2), the flow rate of the first section relative to the moving phase end (Desorbent) between it and the second section is 4.8 mL/min, and the first section and the The flow rate of the extraction port (Extract) of the second section is 2.8 mL/min, the flow rate of the feed end (Feed) between the second section and the third section is 0.4 mL/min, and the first section The flow rate of the three sections relative to the exit (Raffinate) of the second section is 2.4 mL/min.

Moreover, in this embodiment, the switching time of the simulated moving bed system is 2.7 minutes to 4.75 minutes, and preferably, the switching time is 3.125 minutes to achieve the best separation effect. Among them, with the aforementioned production method and environmental parameter settings, the switching time of different simulated moving bed systems is performed, and qualitative and quantitative analysis is performed by high performance liquid chromatography (HPLC). As shown in Figure 10, the number in the figure is the value of the switching time, followed by the letter M is the abbreviation of minutes (min), and the letter E and the letter R are extract and raffinate respectively ) Shorthand. As shown in Figure 10, when the switching time is 2.5 minutes, the time is too short, which makes the flow rate of the stationary phase (Stationary Phase) relative to the mobile phase (Mobile Phase) too fast, which in turn leads to extract and raffinate (Raffinate) all appear the underlying components. In addition, when the switching time is 2.7 minutes, the two target components can be separated, but cichoric acid and caffeoyl tartaric acid can be obtained in the raffinate at the same time. When the switching time is 3.125 minutes, the two target components can be completely separated. And when the switching time is 4.75 minutes, the system can separate the two target components from the raffinate, and separate the impurities when the residence time is greater than 40 minutes. Furthermore, the above extract and raffinate were respectively subjected to quantitative analysis, and the results are shown in Table 3.

Table 3 Data of switching time of simulated moving bed system Switching time (min) Export side Total substance concentration (ppm) Caftaric acid Cichoric acid Total phenolic acid weight content (wt%) Concentration (ppm) Percentage content (%) Concentration (ppm) Percentage content (%) Feed (initial value) 8531.00 252.87 2.96 358.56 4.20 7.71 2.5 Extraction solution (E) 193.49 16.14 8.34 34.20 17.68 26.02 Raffinate (R) 1111.95 21.69 1.95 14.71 1.32 3.27 2.7 Extraction solution (E) 123.73 2.04 1.65 20.95 16.93 18.58 Raffinate (R) 1152.63 33.80 2.93 21.38 1.86 4.79 3.125 Extraction solution (E) 133.83 0.52 0.39 40.43 30.21 30.60 Raffinate (R) 1129.85 38.34 3.39 0.08 0.01 3.40 4.75 Extraction solution (E) 55.66 0.26 0.48 0.25 0.45 0.92 Raffinate (R) 1222.87 38.36 3.14 41.72 3.41 6.55

Please refer to Figure 10 and Table 3 again. When the switching time is 2.7 minutes and 3.125 minutes, relatively pure cichoric acid can be obtained in the extract, which is greatly increased to 16.93% compared to the initial 4.20%. And 30.21%. And to obtain a higher concentration of phenolic acid substances, in the extract (Extract) the total phenolic acid content of 15.58% and 30.60% can be obtained respectively, and in the raffinate (Raffinate) it can obtain 4.79% and 3.40 respectively % Of total phenolic acid content. Therefore, the switching time of the simulated moving bed system can be controlled from 2.7 minutes to 4.75 minutes, preferably 3.125 minutes, so that the two phenolic acids in the echinacea can be effectively separated, thereby improving its purity.

In summary, the method of echinacea phenolic acid substance proposed by the present invention is to add a sample of echinacea into the first solvent and adjust the pH of the environment to 6.1, add the adsorbent decolorizing agent, heat, filter, and stand still After that, the filtrate was obtained. Then, the filtrate is put into a simulated moving bed system for purification and separation to further obtain phenolic acids in the echinacea sample, including cichoric acid and caffeoyl tartaric acid. Among them, the simulated moving bed system is designed with three sections as the configuration, and regulates the volume ratio of ethanol and hydrochloric acid in the mobile phase and the switching time, which can effectively separate cichoric acid and caffeoyl tartaric acid. Thereby, the present invention can improve the separation effect and purity of echinacea phenolic acid substances, and can also improve its production efficiency.

However, the above are only the preferred embodiments of the present invention and are not intended to limit the scope of implementation of the present invention; therefore, all equivalent changes and modifications made without departing from the spirit and scope of the present invention should be covered Within the scope of the patent of the present invention.

S1: Step (1) S2: Step (2) P1~P8: Steps of step (1)

Figure 1 is a schematic diagram of a sample after supercritical extraction in an experimental example of the present invention. Figure 2 is the HPLC chart of the sample after supercritical extraction in the experimental example of the present invention. Figure 3 is a block flow diagram of a preferred embodiment of the present invention. Figure 4 is a block flow diagram of step (1) of the preferred embodiment of the present invention. Figure 5 is a schematic diagram of the sample after step (1) of the preferred embodiment and the experimental example of the present invention. Figure 6 is the HPLC chart of the sample after step (1) of the preferred embodiment and the experimental example of the present invention. Fig. 7 is a configuration design diagram of step (2) of the preferred embodiment of the present invention. Figure 8 is an HPLC chart of the mobile phase in step (2) of a preferred embodiment of the present invention. Fig. 9 is the HPLC chart of the mobile phase in step (2) of the preferred embodiment and the experimental example of the present invention. Fig. 10 is an HPLC chart of the switching time of step (2) of the preferred embodiment and the experimental example of the present invention.

no

S1: Step (1)

S2: Step (2)

Claims (9)

A method for extracting echinacea phenolic acid substances, the steps include: (1) adding a sample of echinacea into a first solvent, the first solvent has ethanol and the pH of the environment is 5.9 ~ 6.3, adding an adsorption decolorization (2) Put the filtrate of step (1) into the simulated moving bed system for purification and separation, and then extract the cichoric acid in the filtrate from the filtrate. The caffeoyl tartaric acid in the filtrate is separated from the raffinate. The method for extracting echinacea substances as described in item 1 of the scope of patent application, wherein, in step (2), the simulated moving bed system is divided into a first section, a second section and a third section The raffinate is obtained from the outlet between the first section and the second section, and the raffinate is obtained from the outlet of the third section relative to the second section. The method for extracting echinacea as described in item 2 of the scope of patent application, wherein, in step (2), the stationary phase of the simulated moving bed system is an alkane with 8 to 30 carbons and multiple hydrophilic Hydroxyl groups modify the surface of silicone. The method for extracting echinacea substances as described in item 3 of the scope of the patent application, wherein, in step (2), the mobile phase of the simulated moving bed system is 95% ethanol and 0.1% hydrochloric acid in volume ratio 30% to 70% mixed solvent. The method for extracting echinacea as described in item 4 of the scope of patent application, wherein, in step (2), the mobile phase of the simulated moving bed system is composed of 95% ethanol and 0.1% hydrochloric acid by volume The ratio is 35% mixed solvent. The method for extracting echinacea substances as described in item 5 of the scope of patent application, wherein, in step (2), the flow rate of the discharge port between the first section and the second section is 2.8 mL /min, the flow rate of the third section relative to the outlet of the second section is 2.4 mL/min, and the switching time of the simulated moving bed system is 2.7 minutes to 4.75 minutes. The method for extracting echinacea phenolic acid as described in item 6 of the scope of patent application, wherein, in step (2), the switching time of the simulated moving bed system is 3.125 minutes. The method for extracting echinacea substances as described in item 7 of the scope of patent application, wherein, in step (1), the first solvent is adjusted with 0.01% hydrochloric acid to adjust its environmental pH to 6.1. The method for extracting echinacea phenolic acid substances according to any one of items 1 to 8 of the scope of patent application, wherein, in step (1), the adsorption decolorizing agent is an activated carbon adsorbent.

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