TW202009472A - Method for creating a standard for distinguishing between plant-derived material containing chlorophyll and at least one of polyphenols or terpenoids, program and device - Google Patents

Abstract

The present invention is a method of creating a standard for distinguishing between plant-derived material containing chlorophyll and at least one of polyphenols or terpenoids, the method of the present invention comprising the following steps: a fluorescent fingerprint information acquisition step, which acquires fluorescence fingerprint information consisting of excitation wavelength of the material whose distinction is known, fluorescence wavelength, and fluorescence intensity data; a step of creating standard, which analyses fluorescent fingerprint information that reflects the amount of components derived from at least one of polyphenol or terpenoid in the material and the amount of components derived from chlorophyll as an index, and creating a standard which indicates a relationship between the material distinction, a component derived from at least one of polyphenol or terpenoid, and a component derived from chlorophyll.

Description

Method, program, and device for creating a criterion for distinguishing materials derived from plants containing at least one of chlorophyll and polyphenols or terpenes

The present invention relates to a method, program, and device for creating a criterion for distinguishing materials derived from plants containing at least one of chlorophyll, polyphenols, or terpenes.

Tobacco (also called tobacco) raw materials include air-dried tobacco raw materials (such as Burley and native species), forced fire-dried tobacco raw materials (such as yellow seeds), and sun-dried tobacco raw materials (such as Oriental species) and other raw materials are distinguished, but they cannot be distinguished visually. Regarding the method of discriminating the classification of tobacco raw materials, a method of discriminating the classification of tobacco raw materials based on the reflectance when the tobacco raw materials are irradiated with visible light has been proposed (Patent Document 1). On the other hand, it is known to use fluorescent fingerprint information to identify or quantify food materials and the like (Non-Patent Document 1).

[Prior Technical Literature] [Patent Literature]

[Patent Literature 1] International Publication No. 2013/132622

[Non-patent literature]

[Non-Patent Document 1] Junichi Sugiyama "Identification and Quantification of Food Using Light Fingerprints", Food and Containers, 2013, Volume 54, No. 5, pages 308 to 315

Materials derived from "plants containing at least one of chlorophyll, polyphenols, or terpenes", such as tobacco raw materials or tea raw materials, change color due to processing steps such as drying or storage conditions, so it is difficult to distinguish visually. Although the method of using visible light to discriminate the distinction of the material described in Patent Document 1 is simple, when the reflection data closely resembles, it may cause a problem in discriminating ability. In fact, the reflectance data obtained for the yellow species of tobacco raw material and the oriental species are very similar (please refer to Fig. 2 of Patent Document 1), so the discrimination between the two is difficult. In view of such a problem, the subject of the present invention is to provide a criterion, a discrimination method and a program for distinguishing materials derived from "plants containing at least one of chlorophyll and polyphenols or terpenes" with good accuracy和装置。 And devices.

The inventors found that the aforementioned problem can be solved by using fluorescent fingerprint information. That is, the aforementioned problem is solved by the following invention.

[1] A method for creating a criterion for distinguishing materials derived from plants containing at least one of chlorophyll and polyphenols or terpenes. The method includes the following steps: fluorescent fingerprint information acquisition step, It is to obtain fluorescence fingerprint information composed of the data of excitation wavelength, fluorescence wavelength, and fluorescence intensity of the aforementioned materials that are known to be known; the step of making a reference will reflect that the aforementioned materials are derived from polyphenols or terpenes The presence of at least one of the components and the fluorescent fingerprint information of the presence of the chlorophyll-derived components are analyzed as indicators, and an ingredient showing at least one of the aforementioned material distinctions, derived from polyphenols or terpenes, and The basis of the relationship between the components derived from chlorophyll.

[2] The method according to [1], wherein the material is tobacco raw material or tea raw material.

[3] The method according to [2], wherein the material is a tobacco raw material, and the above-mentioned classifications are Perry species, yellow species, and oriental species.

[4] The method according to any one of [1] to [3], wherein the material is powder.

[5] The method according to [4], wherein the maximum particle diameter of the material is 1 mm or less.

[6] The method according to any one of the items [1] to [5], wherein the step of acquiring the fluorescent fingerprint information includes: irradiating excitation light of 250 to 370 nm, and measuring the origin of the polyphenol or terpene Fluorescence of 300 to 480 nm of the component of at least one of the above; or irradiation of excitation light of 350 to 480 nm to measure fluorescence of 670 to 700 nm due to the component derived from chlorophyll; or both.

[7] The method according to any one of items [1] to [6], wherein the analysis is multivariate analysis.

[8] The method according to [7], wherein the multivariate analysis is principal component analysis.

[9] The method according to [7], wherein the multivariate analysis is PLS discriminant analysis.

[10] The method according to any one of [1] to [9], wherein the material is tobacco raw material; before the step of obtaining fluorescent fingerprint information, the method further includes: Steps of state adjustment after 60% standing for more than 24 hours.

[11] A method for discriminating the distinction between the aforementioned materials, the method includes the following steps: Step 1, is to obtain fluorescent fingerprint information, the fluorescent fingerprint information is derived from the classification as unknown, including chlorophyll and The data of the excitation wavelength, fluorescence wavelength, and fluorescence intensity of the plant material of at least one of polyphenols or terpenes; Step 2 will reflect at least one of the above materials derived from polyphenols or terpenes Fluorescence fingerprint information of the amount of the present ingredient and the amount of the chlorophyll-derived ingredient are analyzed as an index to obtain the aforementioned material classification, the ingredient derived from at least one of polyphenols or terpenes, and the chlorophyll-derived ingredient The relationship between the ingredients; Step 3 is to prepare the benchmark described in any one of [1] to [10], compare the benchmark with the relationship obtained in Step 2, and judge the distinction of the material.

[12] A program for causing a computer to execute the method described in any one of [1] to [11].

[13] An apparatus for making a criterion for distinguishing materials derived from plants containing at least one of chlorophyll and polyphenols or terpenes, the apparatus is provided with the following means: Means (A1), Obtain fluorescence fingerprint information composed of the data of excitation wavelength, fluorescence wavelength, and fluorescence intensity of the aforementioned materials, which are known; and means (A2), which will reflect the polyphenols or terpenes derived from the aforementioned materials The presence of at least one of the components and the fluorescent fingerprint information of the presence of the chlorophyll-derived components are analyzed as indicators, and an ingredient showing at least one of the aforementioned material distinctions, derived from polyphenols or terpenes, and The basis of the relationship between the components derived from chlorophyll.

[14] An apparatus for distinguishing the classification of materials derived from plants containing at least one of chlorophyll and polyphenols or terpenes as unknown, the apparatus is provided with the following means: as described in the aforementioned item [13] The means (A1) and means (A2); the fluorescent fingerprint information obtaining means (B1) is to obtain the fluorescent fingerprint composed of the data of the excitation wavelength, fluorescent wavelength, and fluorescent intensity of the materials classified as known above. Information; and means (B2), which analyzes the fluorescent fingerprint information that reflects the presence of at least one of components derived from polyphenols or terpenes and the presence of chlorophyll-derived components in the aforementioned materials as indicators , Obtain the relationship between the aforementioned material classification, the component derived from at least one of polyphenols or terpenes, and the component derived from chlorophyll; and the means (C), obtained from the basis obtained by means (A2) and obtained by means (B2) The relationship is compared, and the distinction between the aforementioned distinctions as unknown materials is judged.

According to the present invention, it is possible to provide a standard and discrimination method, program, and device for distinguishing materials derived from plants containing at least one of chlorophyll and polyphenols or terpenes with good accuracy.

1‧‧‧ Peaks due to components derived from polyphenols or components derived from terpenes

3‧‧‧ Peak due to chlorophyll-derived components

A1‧‧‧A method to obtain fluorescent fingerprint information distinguished into known materials

A2‧‧‧ Create a method to show the relationship between materials and components derived from polyphenols

A10‧‧‧fluorescent fingerprint information

A20‧‧‧ benchmark

B1‧‧‧A method to obtain fluorescent fingerprint information distinguished as unknown materials

B2‧‧‧A method to obtain the relationship between materials and components derived from polyphenols

B10‧‧‧fluorescent fingerprint information

B20‧‧‧Relation

C‧‧‧ Means to compare benchmarks and relationships

Fig. 1 is a diagram showing a fluorescent fingerprint in the embodiment.

FIG. 2 is a diagram showing one aspect of using the principal component analysis criterion in the present invention.

FIG. 3 is a diagram showing another aspect of using the principal component analysis criterion in the present invention.

Fig. 4 is a diagram showing an aspect of an apparatus for implementing the present invention.

Figure 5 is a diagram showing another aspect of an apparatus for implementing the present invention

Hereinafter, the present invention will be described in detail. In the present invention, X to Y include X and Y of their end values.

1. How to create a benchmark

It is used as the basis for distinguishing the material derived from plants containing at least one of chlorophyll and polyphenols or terpenes. By obtaining the fluorescent fingerprint information of the material, it will reflect the polyphenol derived from the aforementioned material Fluorescence fingerprint information of the presence amount of at least one of the components of terpenes and the amount of presence of the components derived from chlorophyll is analyzed as an index to obtain the components derived from at least one of the aforementioned material classifications and derived from polyphenols or terpenes , And the relationship between the components derived from chlorophyll, and based on this relationship. This benchmark is also called a pre-established benchmark.

The so-called material derived from a plant containing "chlorophyll" and "at least one of polyphenols or terpenes" (hereinafter also simply referred to as "target material") is to cut, shape, heat, and dry the plant Processed materials or unprocessed plants. The term “terpenoids” refers to a group of natural organic compounds bonded by a plurality of isoprene units, and examples thereof include carotenoids. Chlorophyll and polyphenols or terpenes contained in plants may be metabolized by processing or changes over time. Therefore, the target material system includes chlorophyll or chlorophyll metabolites, polyphenols or polyphenol metabolites, and terpenes or terpene metabolites. Target materials include tobacco raw materials and tea raw materials. The so-called tobacco raw materials refer to raw materials obtained by processing tobacco leaves and stems. Tobacco raw materials may also include stem leaves, middle stems, and regenerated tobacco (that is, the leaves, debris, middle stem chips, fine powders, etc. produced in the factory's operating steps are processed into reusable flakes and other shapes Tobacco material), or any of these mixtures. The so-called tea raw material is a raw material obtained by processing tea leaves and tea stems. The so-called material distinction refers to the distinction based on the properties of the material. Tobacco raw materials can be classified by the drying method, that is, air-dried tobacco raw materials (such as Baili and native species), forced fire-dried tobacco raw materials (such as yellow species), and sun-dried tobacco raw materials (For example, Oriental species). Therefore, the standard for distinguishing whether the tobacco raw material belongs to any one of the Bailey species, the yellow species, and the Oriental species can be cited as one aspect. In addition, regarding the tea raw materials, a standard for distinguishing whether it belongs to any one of sencha, deep-steamed tea, roasted tea, oolong tea, black tea, etc. can be provided as an aspect.

[Steps to obtain fluorescent fingerprint information]

In this step, the fluorescent fingerprint information of the known target material is obtained. The so-called fluorescent fingerprint information refers to a three-dimensional fluorescent pattern composed of data of excitation wavelength, fluorescent wavelength, and fluorescent intensity, which is measured by irradiating excitation light to measure fluorescent wavelength and fluorescent intensity. The measurement is preferably (1) irradiation with excitation light of 250 to 370 nm and measurement of fluorescence from 300 to 480 nm due to components derived from polyphenols or terpenes; or (2) irradiation with excitation light of 350 to 480 nm and measurement of cause Fluorescence from 670 to 700 nm derived from chlorophyll-derived components; or including both (1) and (2). Each wavelength is adjusted appropriately according to the characteristics of the object. For example, for tobacco raw materials, the excitation light wavelength in (1) can be preferably set to 320 to 370 nm, more preferably can be set to 360 nm, and the fluorescent wavelength can be preferably set to 380 to 480 nm, more preferably can be set It is 460 nm, the excitation light wavelength in (2) can be set to 400 nm, and the fluorescence wavelength can be set to 680 nm. Fluorescence from components derived from chlorophyll is fluorescence from chlorophyll and chlorophyll metabolites. The fluorescence derived from the components derived from polyphenols and the components derived from terpenes is also the same.

The properties of the material to be measured are not limited, but it is preferable to pulverize the material to form a uniform powder for measurement. Each component contained in the material is uniformly dispersed, so high-precision measurement can be performed. A well-known machine can be used for pulverization. The maximum particle size is preferably 1 mm or less. The maximum particle size is determined by passing a mesh of a specified size. For example, the tobacco raw material system may include layers, crumbs, and powders, but it is preferable to pulverize it to the aforementioned particle size, and then thoroughly mix them for measurement. The material to be measured is preferably adjusted in advance so that the moisture content becomes constant. For example, if it is a tobacco raw material, it is preferably stored and stored for 24 hours or more under a condition of adjustment (22°C, 60%). The upper limit of the storage time is not limited, but it is preferably within 30 hours.

[Benchmark creation procedure]

In this step, fluorescent fingerprint information reflecting the amount of at least one of the components derived from polyphenols or terpenes and the amount of components derived from chlorophyll in the aforementioned materials is analyzed as an index. The analytical system can use multivariate analysis such as principal component analysis, discriminant analysis, decision tree analysis, hierarchical data analysis, and non-hierarchical clustering analysis. However, it is better to use principal component analysis or PLS discriminant analysis. The so-called principal component analysis is a method of selecting principal components in such a way as to maximize the dispersion of the principal components, and to perform principal component analysis using only explanatory variables and to perform the least squares method between the resulting principal components and the target variables The method of multiple regression analysis. For example, for the principal component analysis of fluorescent fingerprint information, the first principal component (PC1) is used as information about the component derived from polyphenols, the component derived from terpenes, or both, and the third principal component (PC3) is used ) As information about ingredients derived from chlorophyll. If a two-dimensional graph is prepared from the fractions of the first main component and the third component, it can be created to show the material classification, one or both of components derived from polyphenols or components derived from terpenes, and those derived from chlorophyll Two-dimensional diagram of the relationship of the components. In this two-dimensional diagram, by performing grouping to distinguish each material distinction, a reference for each material distinction can be made. Grouping can also use statistical methods, but it is preferably implemented by considering the similarity of the drawing group of unknown raw material samples and known raw material samples. If a plurality of target materials belonging to different divisions are measured, a reference with higher accuracy can be made. In particular, regarding the tobacco raw materials, in the yellow species, the berry species, and the oriental species, the polyphenol-derived components, terpene-derived components, and chlorophyll-derived components are present in different amounts, so they can be made with high accuracy Benchmark. Figure 2 shows an example of a criterion for principal component analysis used to distinguish whether the tobacco raw material belongs to any of yellow, berry, and oriental species. The algorithm used in this step may be a more versatile machine learning algorithm that also corresponds to a non-linear phenomenon, such as support vector machine (SVM), random forest (RF), neural network ( neural network) etc.

PLS regression analysis is a method of selecting principal components in such a way as to maximize the co-dispersion of principal components and target variables. PLS discriminant analysis refers to the analysis of the aforementioned variables as variables of 0 and 1. For PLS discriminant analysis of fluorescent fingerprint information, for example, the clusters of yellow, oriental, and Bailey are used as target variables (1,0,0), (0,1,0), (0,0,1), respectively. Analyze and make individual benchmarks.

2. The method of distinguishing the materials classified as unknown objects

As mentioned earlier, the benchmarks made can be used to distinguish between materials that are unknown. The method includes the following steps.

Step 1: Obtain fluorescent fingerprint information composed of data of excitation wavelength, fluorescent wavelength, and fluorescent intensity that are classified as unknown target materials.

Step 2: Analyze fluorescent fingerprint information reflecting the presence of at least one of polyphenols or terpenes in the aforementioned materials and the presence of chlorophyll-derived components as indicators to obtain polyphenol-derived Or the relationship between the component of at least one of terpenes and the component derived from chlorophyll.

Step 3: Prepare the aforementioned benchmark, compare the benchmark and the relationship obtained in Step 2, and judge the distinction of the material.

Step 1 can be implemented as described in the benchmarking method. In step 2, when principal component analysis is used as the analysis method, for example, the analysis is performed as described in the reference preparation method, and the first principal component (PC1) and the third principal component (PC3) are obtained for the object materials classified as unknown ) Score. Then, in step 3, the score is plotted against the aforementioned benchmark and compared, and the material classification that is classified as unknown target material can be specified.

3. Program

The so-called program refers to the data processing method described according to any vocabulary or description method, regardless of the form of the original code or binary code, etc. In addition, the program can be composed in a single form, but it can also be distributed in the form of a plurality of modules or libraries (Library). In addition, it can also be structured in a way to achieve its function in conjunction with other existing programs.

4. Device (1) Create a device that can be used as a criterion for distinguishing between target materials

The device is provided with the following means: means (A1), which obtains fluorescent fingerprint information composed of data distinguishing the excitation wavelength, fluorescence wavelength, and fluorescence intensity of the aforementioned material known; and means (A2), which is The fluorescent fingerprint information reflecting the amount of at least one of the components derived from polyphenols or terpenes and the amount of the components derived from chlorophyll in the aforementioned materials is analyzed as an indicator to show that the aforementioned materials are distinguished from The basis of the relationship between the components of at least one of polyphenols and terpenes and the components derived from chlorophyll.

Fig. 4 shows an aspect of the device. In the figure, A1 is a means to obtain fluorescent fingerprint information that is classified as a known material, A10 is a fluorescent fingerprint information obtained by this method, and A2 is used as a reference to show the relationship between the material classification and components derived from polyphenols, etc. Means, A20 is based on the benchmark obtained by this means.

(2) Apparatus for distinguishing the classification of unknown target materials

The device is equipped with the following means: the aforementioned means (A1) and means (A2), and the fluorescent fingerprint information obtaining means (B1), which obtains the excitation wavelength, fluorescent wavelength, and fluorescent intensity of the materials classified as known by the foregoing Fluorescent fingerprint information constituted by the data; and means (B2), which will reflect the amount of the presence of at least one component derived from polyphenols or terpenes and the amount of the component derived from chlorophyll in the aforementioned materials Fingerprint information is analyzed as an index to obtain the relationship between the aforementioned material classification, the component derived from at least one of polyphenols or terpenes, and the component derived from chlorophyll; and means (C), obtained by means (A2) The relationship between the reference and the means (B2) is compared, and the aforementioned division is judged as the division of the unknown material.

Fig. 5 shows an aspect of the device. In the figure, A1 and A2 are as defined in Figure 3. B1 is a means to obtain fluorescent fingerprint information distinguished as unknown materials. B10 is a means to obtain fluorescent fingerprint information obtained by this method. B2 is a material distinction and derived from multiple For the relationship between phenol and other components, B20 is the relationship obtained by this method, and C is the method for comparing the aforementioned reference with the relationship.

In the present invention, the device may be configured in the form of hardware, but may also be configured in the form of a combination of function realization means for realizing various functions by software of the computer. The function realization means may include a program module.

[Example] [Example 1]

The following are prepared as the tobacco raw materials classified as known.

Yellow species (America, Tanzania, Italy, Zimbabwe, India, China, Brazil)

Perry (made in Brazil)

Oriental species (from Izmir, from Greece, from Thailand, from China)

The layered material of each raw material was pulverized using a pulverizer to a maximum particle size of 1 mm or less. Obtain the fluorescent fingerprint information of the material (Figure 1). In the first figure, 1 is caused by the peak of the component derived from polyphenol or carotenoid, and 3 is caused by the peak of the component derived from chlorophyll.

The measurement conditions are as follows.

Measuring device: F-7000 (fluorescent fingerprint measuring device, manufactured by Hitachi High Tech Science)

Measurement method: reflection method (Front Face)

Measurement conditions: excitation light 200 to 600 nm, fluorescent light 200 to 900 nm, resolution capability 5 nm, slit width 5 nm, photomultiplier tube sensitivity 700

For the obtained fluorescent fingerprint information, unnecessary wavelengths irrelevant to the target component are deleted. Specifically, non-fluorescent component removal processing, scattered light removal processing, and low-sensitivity area deletion processing are performed. Furthermore, the fluorescent fingerprint information is normalized and automatically adjusted (autoscale). The pre-processed fluorescent fingerprint information was analyzed using the software "Matlab" for the main components, using PC1 as the component derived from polyphenols and carotenoid, and PC3 as the component derived from chlorophyll. The basis of the relationship between the material classification, the polyphenol-derived component and the carotenoid-derived component, and the chlorophyll-derived component. The results are shown in Figure 2. As shown in Figure 2, tobacco materials of the same division are plotted at similar positions. This is grouped into, for example, visually grouped into individual divisions, thereby creating a reference.

[Example 2]

For the pre-processed fluorescent fingerprint information obtained in Example 1, PLS discriminant analysis was performed using software ("PLS TOOLBOX" manufactured by Eigenvector Research, Inc.), and the clusters of yellow, oriental, and Bailey were used as target variables (1 ,0,0), (0,1,0), (0,0,1) and analyze them to create separate benchmarks. Furthermore, in order to verify the validity of the benchmark, the 25 samples classified as known are judged based on the benchmark, and whether the research is consistent with the actual samples. As a result, 24 samples were confirmed to be consistent (96% discriminant score), which shows that the accuracy of the benchmark is high.

[Example 3]

Black tea, roasted tea, and sencha are prepared as the raw materials of tea that are classified as known. Each raw material was pulverized using a pulverizer to a maximum particle size of 1 mm or less. The fluorescent fingerprint information of the material was obtained under the same conditions as in Example 1.

[Example 4]

For the pre-processed fluorescent fingerprint information obtained in Example 3, PLS discriminant analysis was performed using software ("PLS TOOLBOX" manufactured by Eigenvector Research, Inc.), and the clusters of black tea, roasted tea, and simmered tea were used as target variables ( 1,0,0), (0,1,0), (0,0,1) and analyzed to create separate benchmarks (Figure 3). In addition, in order to verify the validity of the benchmark, 21 samples classified as known based on the benchmark are judged, and whether the research is consistent with the actual samples. As a result, 20 samples were confirmed to be consistent (96% discriminant score), which shows that the accuracy of the benchmark is high.

Claims (14)

A method for creating a method for determining the basis for distinguishing materials derived from plants containing at least one of chlorophyll and polyphenols or terpenes. The method includes the following steps: a fluorescent fingerprint information obtaining step, which is obtained by Distinguish into fluorescent fingerprint information composed of known excitation wavelength, fluorescence wavelength, and fluorescence intensity data of the aforementioned materials; the step of making a reference will reflect at least one of the aforementioned materials derived from polyphenols or terpenes Fluorescence fingerprint information of the amount of the present component and the amount of the chlorophyll-derived component is analyzed as an index, and a component showing at least one of the aforementioned material classification, derived from polyphenols or terpenes, and chlorophyll-derived component is prepared The benchmark of the relationship of the ingredients. The method as described in item 1 of the patent application scope, wherein the aforementioned material is tobacco raw material or tea raw material. The method as described in item 2 of the patent application scope, wherein the aforementioned material is tobacco raw material, and the aforementioned classification is divided into Perry, yellow, and oriental species. The method according to any one of items 1 to 3 of the patent application range, wherein the aforementioned material is a powder. The method as described in item 4 of the patent application scope, wherein the maximum particle size of the aforementioned material is 1 mm or less. The method according to any one of items 1 to 5 of the patent application scope, wherein the step of acquiring the fluorescent fingerprint information includes: irradiating excitation light of 250 to 370 nm, and measuring at least the cause derived from polyphenols or terpenes Fluorescence of 300 to 480 nm of one of the components; or irradiation of excitation light of 350 to 480 nm to measure fluorescence of 670 to 700 nm due to the component derived from chlorophyll; or both. The method according to any one of items 1 to 6 of the patent application scope, wherein the foregoing analysis is a multivariate analysis. The method as described in item 7 of the patent application scope, wherein the aforementioned multivariate analysis is principal component analysis. The method as described in item 7 of the patent application scope, wherein the aforementioned multivariate analysis is PLS discriminant analysis. The method as described in any one of items 1 to 9 of the patent application scope, wherein the aforementioned material is tobacco raw material; before the step of obtaining fluorescent fingerprint information, the method further includes: placing the tobacco raw material at 22°C, 60% static Steps to adjust the status for more than 24 hours. A method for discriminating the distinction between the aforementioned materials. The method includes the following steps: Step 1. Obtain fluorescent fingerprint information. The fluorescent fingerprint information is derived from chlorophyll and polyphenols. Data of the excitation wavelength, fluorescence wavelength, and fluorescence intensity of the material of the plant of at least one of the terpenes; Step 2 will reflect the composition of at least one of polyphenols or terpenes in the aforementioned material The fluorescence fingerprint information of the presence amount and the presence amount of the chlorophyll-derived component is analyzed as an index to obtain the relationship between the aforementioned material classification, the component derived from at least one of polyphenols or terpenes, and the component derived from chlorophyll ; Step 3 is the basis for preparing any one of the items 1 to 10 of the patent application scope, and compares the relationship between the basis and the step 2 to determine the distinction between the materials. A program used to make a computer execute the method described in any one of items 1 to 11 of the patent application. An apparatus for making a basis for distinguishing materials derived from plants containing at least one of chlorophyll and polyphenols or terpenes, the apparatus is provided with the following means: Means (A1) Fluorescent fingerprint information composed of known excitation wavelength, fluorescence wavelength, and fluorescence intensity data of the aforementioned materials; and means (A2), which will reflect at least one of the aforementioned materials derived from polyphenols or terpenes Fluorescence fingerprint information of the amount of the present component and the amount of the chlorophyll-derived component is analyzed as an index, and a component showing at least one of the aforementioned material distinction, polyphenols or terpenes, and chlorophyll-derived component are prepared The benchmark of the relationship of the ingredients. A device that distinguishes between materials that are unknown and originates from plants containing at least one of chlorophyll and polyphenols or terpenes. The device is equipped with the following means: the means described in item 13 of the patent application scope (A1) and means (A2); the fluorescent fingerprint information obtaining means (B1) is to obtain the fluorescent fingerprint information composed of the data of excitation wavelength, fluorescent wavelength, and fluorescent intensity distinguished into known materials; and Means (B2) is to analyze the fluorescent fingerprint information reflecting the presence of at least one of the components derived from polyphenols or terpenes and the presence of the components derived from chlorophyll in the aforementioned materials as indicators to obtain the aforementioned Material classification, the relationship between components derived from at least one of polyphenols or terpenes, and components derived from chlorophyll; and means (C), the relationship between the basis obtained by means (A2) and the means obtained by means (B2) In contrast, the aforementioned distinction is judged as the distinction of unknown materials.

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