TW201922117A - Composition for improving quality of taste comprising allulose - Google Patents

Composition for improving quality of taste comprising allulose Download PDF

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TW201922117A
TW201922117A TW107139915A TW107139915A TW201922117A TW 201922117 A TW201922117 A TW 201922117A TW 107139915 A TW107139915 A TW 107139915A TW 107139915 A TW107139915 A TW 107139915A TW 201922117 A TW201922117 A TW 201922117A
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weight
composition
parts
naringin
sweetener
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TWI691278B (en
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朴政圭
沈東錫
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南韓商Cj第一製糖股份有限公司
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/86Addition of bitterness inhibitors
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30Artificial sweetening agents
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30Artificial sweetening agents
    • A23L27/33Artificial sweetening agents containing sugars or derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/84Flavour masking or reducing agents
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
    • A23V2250/21Plant extracts

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Seasonings (AREA)

Abstract

The present disclosure relates to a composition for improving quality of taste, which contains allulose, and a preparation method thereof.

Description

用於改善口感品質之包含阿洛酮糖之組成物    Composition containing ptoseose for improving taste quality   

本揭示係有關於一種用於製備甜味劑之組成物,其包含柚皮苷、經酶催化改質的甜菊萃、及涵括阿洛酮糖的醣。特定言之,本揭示係有關於一種用於製備甜味劑之組成物的製備方法;一種用於製備甜味劑之方法;一種包含用於製備甜味劑之組成物的甜味劑;一種用於改善辛辣味或苦味之組成物;及一種用於改善辛辣味或苦味之方法。 The present disclosure relates to a composition for preparing a sweetener, which comprises naringin, an enzymatically modified stevia extract, and a sugar containing psicose. Specifically, the present disclosure relates to a method for preparing a composition for preparing a sweetener; a method for preparing a sweetener; a sweetener comprising a composition for preparing a sweetener; Composition for improving spicy or bitter taste; and a method for improving spicy or bitter taste.

甜味劑為食品,及添加用來給食物賦與甜味的食品添加劑,及定義於由食品藥物管理局FDA發行的「食品添加劑詞彙」中。甜味劑能被廣義地分類為以碳水化合物為主的甜味劑及非以碳水化合物為主的甜味劑,及長時間以來,已代表性地使用糖(蔗糖)。晚近,於上述以碳水化合物為主的甜味劑中,糖及果糖被過度消費,已造成社會問題,諸如各種成人疾病包括肥胖的發生率增加(韓國專利公告案第10-1658403號)。因此,需要發展下一代甜味劑以替代以碳水化合物為主的甜味劑,諸如糖。 Sweeteners are foods, and food additives added to impart sweetness to food, and are defined in the "Food Additive Glossary" issued by the Food and Drug Administration FDA. Sweeteners can be broadly classified into carbohydrate-based sweeteners and non-carbohydrate-based sweeteners, and sugar (sucrose) has been typically used for a long time. Recently, among the above-mentioned carbohydrate-based sweeteners, sugar and fructose have been excessively consumed, which has caused social problems such as an increase in the incidence of various adult diseases including obesity (Korean Patent Publication No. 10-1658403). Therefore, there is a need to develop next-generation sweeteners to replace carbohydrate-based sweeteners, such as sugar.

阿洛酮糖乃一種天然糖,微量存在於糖蜜及葡萄糖的反應期 間,而其甜度一般已知為糖甜度之約70%。不似糖及果糖,阿洛酮糖為傳統上廣泛使用的雙醣,有其優點在於阿洛酮糖能用作為零卡甜味劑,原因在於其於體內不被代謝之故。此外,阿洛酮糖抑制體內體脂的生成,極少有發展出成人疾病包括肥胖的風險(韓國專利申請公開案第10-2017-0030524號)。 Alloxulose is a natural sugar, which is present in trace amounts during the reaction between molasses and glucose, and its sweetness is generally known as about 70% of the sugar sweetness. Unlike sugar and fructose, ptoseose is a traditionally widely used disaccharide, which has the advantage that it can be used as a zero calorie sweetener because it is not metabolized in the body. In addition, alloxulose inhibits the production of body fat in the body, and there is very little risk of developing adult diseases including obesity (Korean Patent Application Publication No. 10-2017-0030524).

因此,因其各方面之優異與傑出,阿洛酮糖引人矚目作為下一代甜味劑。然而,阿洛酮糖有問題,出在於就感官知覺而言,其難以使用作為甜味劑,原因在於不似習知醣類,當阿洛酮糖實際上用作為甜味劑時,產生了辛辣味及苦味。 Therefore, due to its superiority and excellence in all aspects, psicose is attracting attention as a next-generation sweetener. However, there is a problem with ptoseose, which is that it is difficult to use as a sweetener in terms of sensory perception, because unlike conventional sugars, it is produced when ptoseose is actually used as a sweetener. Spicy and bitter.

本案發明人徹底進行研究以意圖解決前述問題,及結果,藉由確認當阿洛酮糖、柚皮苷、及經酶催化改質的甜菊萃被組合添加時,因添加阿洛酮糖作為甜味劑所致而可能發生的感官知覺問題能被有效地解決,本案發明人已完成了本揭示。 The inventors of the present case have conducted thorough research to solve the foregoing problems, and as a result, by confirming that when allosexose, naringin, and the enzyme-catalyzed stevia extract were added in combination, allosexose was added as a sweetener. The sensory perception problem that may occur due to the flavor can be effectively solved, and the present inventor has completed the present disclosure.

本揭示之一目的係提供一種用於製備甜味劑之組成物,其包含柚皮苷、經酶催化改質的甜菊萃、及涵括阿洛酮糖的醣。 It is an object of the present disclosure to provide a composition for preparing a sweetener, which comprises naringin, an enzymatically modified stevia extract, and a sugar containing alloxulose.

本揭示之另一目的係提供一種用於製備甜味劑之組成物的製備方法,其包含混合柚皮苷、經酶催化改質的甜菊萃、及涵括阿洛酮糖的醣之步驟。 Another object of the present disclosure is to provide a method for preparing a composition for preparing a sweetener, which comprises the steps of mixing naringin, an enzyme-catalyzed stevia extract, and a sugar containing phloxulose.

本揭示之又另一目的係提供一種用於製備甜味劑之方法,其包含混合柚皮苷、經酶催化改質的甜菊萃、及涵括阿洛酮糖的醣之步驟。 Yet another object of the present disclosure is to provide a method for preparing a sweetener, which comprises the steps of mixing naringin, an enzymatically modified stevia extract, and a sugar containing ptoseose.

本揭示之又另一目的係提供一種包含該用於製備甜味劑之組成物的甜味劑。 Yet another object of the present disclosure is to provide a sweetener comprising the composition for preparing a sweetener.

本揭示之又另一目的係提供一種用於改善辛辣味或苦味之組成物,其包含柚皮苷、經酶催化改質的甜菊萃、及涵括阿洛酮糖的醣。 Yet another object of the present disclosure is to provide a composition for improving a spicy or bitter taste, which comprises naringin, an enzymatically modified stevia extract, and a sugar containing psicose.

本揭示之又另一目的係提供一種用於改善辛辣味或苦味之方法,其包含混合柚皮苷、經酶催化改質的甜菊萃、及涵括阿洛酮糖的醣之步驟。 Yet another object of the present disclosure is to provide a method for improving a spicy or bitter taste, which comprises the steps of mixing naringin, an enzymatically modified stevia extract, and a sugar containing alloxulose.

藉由組合使用阿洛酮糖、柚皮苷、及經酶催化改質的甜菊萃,本揭示提供一種用於改善口感品質的新穎組成物,其藉由減少阿洛酮糖的辛辣味及/或苦味,能够改善口感品質及補償甜度。 The present disclosure provides a novel composition for improving mouthfeel quality by using a combination of psicose, naringin, and stevia extract that is catalytically modified by enzymes, by reducing the spicy taste of psicose and / Or bitterness, can improve the taste quality and compensate for sweetness.

[較佳實施例之詳細說明]     [Detailed description of the preferred embodiment]    

後文中,將以細節描述本揭示。同時,本文中揭示的解說及具體實施例中之各者可應用至其它解說及具體實施例。換言之,本文中揭示的各項因素之全部組合皆係屬於本揭示之範疇。又復,本揭示之範圍不 應受後文提供之特定揭示所限。 Hereinafter, this disclosure will be described in detail. At the same time, each of the explanations and specific embodiments disclosed herein can be applied to other explanations and specific embodiments. In other words, all combinations of factors disclosed in this article belong to the scope of this disclosure. Again, the scope of this disclosure should not be limited by the specific disclosure provided below.

阿洛酮糖引人矚目作為糖的替代甜味劑,其具有0千卡/公克(kcal/g)至0.2千卡/公克的低卡含量,因而具有優點在於其提供習知醣類(諸如糖等)的0%至5%之低卡含量。然而,不似為人高度喜好的習知醣類(蔗糖、果糖),阿洛酮糖有產生辛辣味及苦味的問題。就此方面而言,於本揭示中,確證藉由組合使用柚皮苷、經酶催化改質的甜菊萃、及阿洛酮糖作為醣,能改善阿洛酮糖的口感品質,換言之,能減少辛辣味及苦味,及補償甜味,此等係由本案發明人首度發現。 Pyrosose is attracting attention as an alternative sweetener for sugar, which has a low calorie content of 0 kcal / g to 0.2 kcal / g, and therefore has the advantage of providing conventional sugars such as Sugar, etc.) from 0% to 5%. However, unlike conventional sugars (sucrose, fructose) that are highly favored, psicose has the problem of producing spicy and bitter tastes. In this regard, in the present disclosure, it was confirmed that the combination of naringin, enzymatically modified stevia extract, and allosene as a sugar can improve the taste quality of alloselose, in other words, it can reduce Spicy and bitter flavors, and compensating sweetness, were first discovered by the inventors of the present case.

本揭示之一態樣提供一種用於製備甜味劑之組成物,其包含醣、柚皮苷、及經酶催化改質的甜菊萃。 One aspect of the present disclosure provides a composition for preparing a sweetener, comprising a sugar, naringin, and an enzyme-catalyzed stevia extract.

於本揭示中,用於製備甜味劑之組成物可呈液體或粉末形式。 In this disclosure, the composition used to prepare the sweetener may be in liquid or powder form.

於本揭示中,「阿洛酮糖」乃具有C6H12O6之化學式及180.16分子量的一類型醣,已知小量存在於無花果、葡萄等。阿洛酮糖包括D-阿洛酮糖及L-阿洛酮糖兩者。市售阿洛酮糖可被購買使用,或可使用透過化學方法或微生物學方法製備的阿洛酮糖。 In the present disclosure, "psicose" is a type of sugar having a chemical formula of C 6 H 12 O 6 and a molecular weight of 180.16, which is known to exist in small amounts in figs, grapes, and the like. D-psicose includes both D-psicose and L-psicose. Commercially available allosexose can be purchased for use, or allosexose can be prepared by chemical or microbiological methods.

阿洛酮糖可以適量涵括於用於製備甜味劑之組成物,及特定言之,以用於製備甜味劑之組成物的100重量份總重為基準計,其含量可以是70至99.99重量份,75至99.99重量份,80至99.99重量份,85至99.99重量份,90至99.99重量份,95至99.99重量份,98至99.99重量份,99至99.99重量份,95至99.98重量份,或99.98重量份,但阿洛酮糖之含量並非受此所限。 Dose can be included in a proper amount in a composition for preparing a sweetener, and specifically, based on a total weight of 100 parts by weight of the composition for preparing a sweetener, the content may be 70 to 99.99 parts by weight, 75 to 99.99 parts by weight, 80 to 99.99 parts by weight, 85 to 99.99 parts by weight, 90 to 99.99 parts by weight, 95 to 99.99 parts by weight, 98 to 99.99 parts by weight, 99 to 99.99 parts by weight, 95 to 99.98 parts by weight Parts, or 99.98 parts by weight, but the content of psicose is not limited by this.

此外,阿洛酮糖可呈液體形式,或呈粉末及晶體形式。當阿洛酮糖係呈液體形式時,以阿洛酮糖的100重量份總重為基準計,其可具有65至80重量份、65至78重量份、68至78重量份、68至75重量份、68至73重量份、或70至72重量份之固體含量,及藉與其它原料混合,阿洛酮糖可呈稀釋形式或濃縮形式使用。此外,以100重量份固體含量為基準計,純阿洛酮糖的雜質可於85至99.5重量份、85至98重量份、90至98重量份、93至98重量份、或95至98重量份之範圍。當阿洛酮糖係呈粉末及晶體形式時,以阿洛酮糖的100重量份總重為基準計,純阿洛酮糖的純度可於90重量份或以上、95重量份或以上、或98重量份或以上之範圍,及藉由與其它原料混合,阿洛酮糖可呈稀釋形式使用。 In addition, ptoseose can be in liquid form or in powder and crystalline form. When the psicose system is in a liquid form, it may have 65 to 80 parts by weight, 65 to 78 parts by weight, 68 to 78 parts by weight, 68 to 75 based on 100 parts by weight of the total weight of psicose The solid content of parts by weight, 68 to 73 parts by weight, or 70 to 72 parts by weight, and by mixing with other raw materials, the phloseose can be used in a diluted form or a concentrated form. In addition, based on 100 parts by weight of solid content, impurities of pure allosene can be 85 to 99.5 parts by weight, 85 to 98 parts by weight, 90 to 98 parts by weight, 93 to 98 parts by weight, or 95 to 98 parts by weight. Range of shares. When the psicose is in powder and crystal form, the purity of the psicose can be 90 parts by weight or more, 95 parts by weight or more, based on 100 parts by weight of the total weight of the psic material. In the range of 98 parts by weight or more, and by mixing with other raw materials, the phloseose can be used in a diluted form.

於本揭示之用於製備甜味劑之組成物中,阿洛酮糖以外之醣可以混合糖之形式涵括。特定言之,阿洛酮糖可以是呈與選自於由下列所組成之組群中之一種或多種醣的混合糖形式:單醣類、雙醣類、寡醣類、及阿洛酮糖以外的糖醇類,但阿洛酮糖非受此所限。最後,可進一步包括額外成分,只要其可以適量涵括於本揭示之用於製備甜味劑之組成物即可。本揭示之用於製備甜味劑之組成物可包括額外糖及/或果糖,或可不包括。 In the composition for the preparation of the sweetener of the present disclosure, sugars other than psicose may be included in the form of mixed sugars. In particular, the psicose may be in the form of a mixed sugar with one or more sugars selected from the group consisting of: monosaccharides, disaccharides, oligosaccharides, and psicose Other sugar alcohols, but psicose is not limited by this. Finally, additional ingredients may be further included as long as they can be included in a suitable amount in the composition for preparing a sweetener of the present disclosure. The compositions used to make sweeteners of the present disclosure may include additional sugars and / or fructose, or may not.

此外,涵括阿洛酮糖的醣可只包括阿洛酮糖作為醣。 In addition, the sugar that encompasses psicose may include only psicose as the sugar.

本揭示之用於製備甜味劑之組成物可以是其口感品質經改善者。於本揭示中,口感品質係指於感覺(諸如口味感覺)中決定的品質,換言之,其係指口味的差異,而可包括甜味、酸味、鹹味、苦味等。 The composition for preparing a sweetener of the present disclosure may be one with improved taste quality. In the present disclosure, the taste quality refers to a quality determined in a feeling (such as a taste feeling), in other words, it refers to a difference in taste, and may include sweetness, sourness, saltiness, bitterness, and the like.

於本揭示中,口感品質可以是辛辣味及/或苦味,及更特別地,可以是因阿洛酮糖所致而產生的辛辣味及/或苦味。當阿洛酮糖被添加至食物作為甜味劑時,具有因添加阿洛酮糖所致而產生的辛辣味或苦味問題。然而,於本揭示之用於製備甜味劑之組成物中,於其中柚皮苷及經酶催化改質的甜菊萃係與阿洛酮糖混合在一起,使得辛辣味及苦味減少,及因而該組成物可被使用作為具有低卡含量的優異甜味劑。 In the present disclosure, the taste quality may be a spicy and / or bitter taste, and more particularly, may be a spicy and / or bitter taste caused by psicose. When allosexose is added to foods as a sweetener, there is a problem of spicy or bitter taste caused by adding allosexose. However, in the composition for preparing a sweetener of the present disclosure, naringin and an enzymatically modified stevia extract system are mixed with allosexose to reduce the spicy and bitter taste, and thus This composition can be used as an excellent sweetener having a low calorie content.

比較不涵括柚皮苷及經酶催化改質的甜菊萃之用於製備甜味劑之組成物,本揭示之用於製備甜味劑之組成物可具有改良之甜味品質。 The composition for preparing sweeteners that does not include naringin and the enzyme-catalyzed stevia extract, the composition for preparing sweeteners of the present disclosure may have improved sweetness qualities.

於本揭示中,甜味品質係指甜度。於本揭示之用於製備甜味劑之組成物中,阿洛酮糖、柚皮苷、及經酶催化改質的甜菊萃係組合涵括,及因此,比較不涵括柚皮苷及經酶催化改質的甜菊萃之用於製備甜味劑之組成物,其甜味品質喜好可予改善。 In this disclosure, sweetness quality refers to sweetness. In the composition for the preparation of sweeteners disclosed in the present disclosure, alloxulose, naringin, and the stevia extract modified by enzyme catalysis are included, and therefore, naringin and naringin are relatively excluded. The composition of enzyme-catalyzed stevia extract used to prepare sweeteners can improve the sweet taste quality preference.

於本揭示中,柑橘屬萃取物可含有柚皮苷。柚皮苷乃具有C27H32O14化學式的一型類黃酮,且係呈黃酮與鼠李糖及葡萄糖組合的形式。柚皮苷乃一種成分,其均勻地出現在以柑橘屬為主的植物的樹皮、果汁、及種子中。此外,因柚皮苷的含量豐富,故柚皮苷可用作為以柑橘屬為主的萃取物的指標物質。又復,除了柚皮苷之外,多酚類(檸檬烯、抗壞血酸、及柚皮苷除外)可進一步涵括於柑橘屬萃取物中。 In the present disclosure, the citrus extract may contain naringin. Naringin is a type flavonoid having the chemical formula of C 27 H 32 O 14 and is in the form of a combination of flavones, rhamnose and glucose. Naringin is a component that occurs uniformly in the bark, fruit juice, and seeds of citrus-based plants. In addition, because naringin is rich in content, naringin can be used as an index substance of citrus-based extracts. Furthermore, in addition to naringin, polyphenols (except limonene, ascorbic acid, and naringin) can be further included in the citrus extract.

檸檬烯乃具有C10H16化學式的材料,於其中重複兩個異戊間二烯單元,且屬一類型的萜烯。檸檬烯為主要出現在以柑橘屬為主的植物的樹皮中之香氣成分,因而可被用作為柑橘屬萃取物的香氣指標。 Limonene is a material with a chemical formula of C 10 H 16 in which two isoprene units are repeated and is a type of terpene. Limonene is an aroma component mainly found in the bark of plants mainly composed of citrus, so it can be used as an aroma index of citrus extracts.

抗壞血酸之含量可以是柚皮苷含量的5倍至20倍,8倍至15 倍,或10倍至12倍,但非受此所限。 The content of ascorbic acid may be 5 to 20 times, 8 to 15 times, or 10 to 12 times the content of naringin, but is not limited thereto.

柚皮苷除外的多酚之含量可以是柚皮苷含量的4倍至20倍,5倍至14倍,或6倍至12倍,但非受此所限。 The content of polyphenols other than naringin may be 4 to 20 times, 5 to 14 times, or 6 to 12 times the content of naringin, but it is not limited thereto.

為了本揭示之目的,柚皮苷可以是柑橘屬萃取物中所含的柚皮苷,但非限制性。此外,組成物中之柚皮苷含量使得苦味不被表達,及特別,可以是0.02%(W/W)或以下,但非受此所限。 For the purposes of this disclosure, naringin may be, but is not limited to, naringin contained in a citrus extract. In addition, the naringin content in the composition prevents bitterness from being expressed, and in particular, may be 0.02% (W / W) or less, but is not limited thereto.

於本揭示中,「柑橘屬萃取物」可包括萃取物本身、使用萃取物加工的純化產物、及使用萃取物可生成的全配方萃取物(例如,藉由對應於柑橘屬之植物的萃取處理所得的萃取物、萃取物的稀釋或濃縮產物、藉由乾燥萃取物所得的乾萃取物、萃取物之部分純化產物或純化產物、其混合物等)。萃取物之萃取方法並無特殊限制,而可根據業界之常用方法萃取。萃取方法之非限制性實施例可包括熱水萃取法、超音波萃取法、過濾法、及回流萃取法;而此等方法可單獨進行或兩種或更多種組合進行。 In this disclosure, "citrus extract" may include the extract itself, a purified product processed using the extract, and a fully-formulated extract that can be generated using the extract (e.g., by an extraction treatment of a plant corresponding to the citrus The obtained extract, a diluted or concentrated product of the extract, a dry extract obtained by drying the extract, a partially purified product of the extract or a purified product, a mixture thereof, etc.). The extraction method of the extract is not particularly limited, and can be extracted according to a method commonly used in the industry. Non-limiting examples of the extraction method may include a hot water extraction method, an ultrasonic extraction method, a filtration method, and a reflux extraction method; and these methods may be performed individually or in a combination of two or more.

柑橘屬萃取物及柑橘屬萃取物中所含的柚皮苷可以呈液體形式或粉末形式。 The citrus extract and naringin contained in the citrus extract may be in a liquid form or a powder form.

柑橘屬萃取物之含量可以是,以阿洛酮糖之100重量份乾固體含量為基準計,0.0001至1重量份、0.0001至0.5重量份、0.0001至0.1重量份、0.001至1重量份、0.001至0.5重量份、0.001至0.1重量份、0.001至0.05重量份、0.001至0.01重量份、0.001至0.002重量份、0.0014至0.002重量份、0.002至0.1重量份、0.002至0.05重量份、0.002至0.01重量份、0.002至0.008重量份、或0.004至0.008重量份,但柑橘屬萃取物之含量並非受此所限。 The content of the citrus extract may be 0.0001 to 1 part by weight, 0.0001 to 0.5 part by weight, 0.0001 to 0.1 part by weight, 0.001 to 1 part by weight, 0.001 based on 100 parts by weight of dry solids content of alloxulose. To 0.5 parts by weight, 0.001 to 0.1 parts by weight, 0.001 to 0.05 parts by weight, 0.001 to 0.01 parts by weight, 0.001 to 0.002 parts by weight, 0.0014 to 0.002 parts by weight, 0.002 to 0.1 parts by weight, 0.002 to 0.05 parts by weight, 0.002 to 0.01 Parts by weight, 0.002 to 0.008 parts by weight, or 0.004 to 0.008 parts by weight, but the content of the citrus extract is not limited thereto.

此外,以阿洛酮糖之100重量份乾固體含量為基準,柑橘屬萃取物中之柚皮苷的含量可以是10x10-5至10x10-6重量份,但非受此所限。 In addition, the content of naringin in the citrus extract may be 10x10 -5 to 10x10 -6 parts by weight based on 100 parts by weight of dry solids content of the psicose, but is not limited thereto.

特定言之,以液體阿洛酮糖之100重量份之乾固體含量為基準,柚皮苷的含量可以是0.00002至0.00006重量份、0.000025至0.00006重量份、或0.00003至0.00006重量份;及以100重量份之結晶阿洛酮糖之乾固體含量為基準,柚皮苷的含量可以是0.00001至0.00002重量份、0.00001至0.000018重量份、或0.00001至0.000016重量份,但非受此所限。 In particular, the content of naringin may be 0.00002 to 0.00006 parts by weight, 0.000025 to 0.00006 parts by weight, or 0.00003 to 0.00006 parts by weight, based on 100 parts by weight of the dry solids content of liquid alloseneose; The dry solids content of the crystalline ptoseose by weight parts is based on, and the content of naringin can be 0.00001 to 0.00002 parts by weight, 0.00001 to 0.00018 parts by weight, or 0.00001 to 0.00016 parts by weight, but is not limited thereto.

於本揭示中,「甜菊萃」乃屬於菊科(Asteraceae)及紫斑風鈴草目(Campanula punctata)的多年生雙子葉青草,及其學名為菊科甜菊屬(Stevia rebaudiana)。此外,已知甜菊萃被使用在花草茶、飲料、中草藥、健康補充品等中。又復,因甜菊萃葉含有大量甜味成分,諸如甜菊苷(stevioside)及甜葉菊苷(rebaudioside),故其可用作為天然甜味劑。「經酶催化改質的甜菊萃」係指已進行酶催化處理以改善口感品質諸如甜菊萃萃取物中產生的苦味的甜菊萃,及使用酶諸如α-葡萄糖基轉移酶等。 In this disclosure, "Stevia extract" is a perennial dicotyledonous grass belonging to the Asteraceae and Campanula punctata, and its scientific name is Stevia rebaudiana. In addition, it is known that stevia extract is used in herbal tea, beverages, Chinese herbal medicine, health supplements, and the like. Moreover, because the stevia extract leaves contain a large amount of sweet components, such as stevioside and rebaudioside, it can be used as a natural sweetener. "Enzyme-modified stevia extract" refers to stevia extract that has been subjected to an enzyme-catalyzed treatment to improve the taste quality, such as the bitter taste produced in the stevia extract, and the use of enzymes such as α-glucosyltransferase.

於本揭示中,可購買或使用市售甜菊萃或經酶催化改質的甜菊萃,或可使用藉由在自然界收穫或栽培所得者,及較佳係主要使用甜菊萃的葉部,但非受此所限。 In this disclosure, a commercially available stevia extract or an enzyme-catalyzed stevia extract may be purchased or used, or one obtained by harvesting or cultivation in nature, and preferably the leaves mainly using stevia extract, but not Limited by this.

於本揭示中,甜菊萃萃取物可藉使用純水、C1至C4低碳醇類、或其混合溶劑萃取甜菊萃而獲得。特定言之,攪拌萃取及超音波萃取係使用純水及低碳醇類,諸如乙醇及甲醇(具有甜菊萃重量之約兩倍至五倍的體積)於50℃至70℃進行;藉由使用於100℃的熱水萃取法連續萃取2至3 次而獲得所得產物;獲得的所得產物通過濾紙過濾之後,以旋轉真空蒸發器去除濾液;殘餘物接受真空冷凍乾燥及熱風乾燥;及藉此可獲得甜菊萃萃取物。 In the present disclosure, the stevia extract can be obtained by extracting the stevia extract with pure water, C 1 to C 4 lower alcohols, or a mixed solvent thereof. Specifically, stirred extraction and ultrasonic extraction are performed at 50 ° C to 70 ° C using pure water and low-carbon alcohols such as ethanol and methanol (having a volume of about two to five times the weight of stevia extract); The obtained product was continuously extracted 2 to 3 times by a hot water extraction method at 100 ° C. After the obtained product was filtered through filter paper, the filtrate was removed by a rotary vacuum evaporator; the residue was subjected to vacuum freeze-drying and hot air drying; A stevia extract is obtained.

此外,本揭示之甜菊萃萃取物可包括藉萃取處理獲得的萃取物、萃取物之稀釋或濃縮產物、藉乾燥萃取物獲得的乾萃取物、及萃取物的部分純化產物或純化產物中之一者或多者。 In addition, the stevia extract of the present disclosure may include one of an extract obtained by an extraction process, a diluted or concentrated product of the extract, a dry extract obtained by a dry extract, and a partially purified product or a purified product of the extract. Or more.

經酶催化改質的甜菊萃可呈液體或粉末形式。 Enzymatically modified stevia extract can be in liquid or powder form.

於本揭示中,以阿洛酮糖之100重量份固體含量為基準計,經酶催化改質的甜菊萃之含量可以是0.001至1重量份,0.001至0.5重量份,0.001至0.2重量份,0.001至0.19重量份,0.001至0.17重量份,0.001至0.15重量份,0.002至1重量份,0.002至0.5重量份,0.002至0.2重量份,0.002至0.19重量份,0.002至0.17重量份,0.002至0.15重量份,0.002至0.13重量份,0.005至0.2重量份,0.005至0.19重量份,0.005至0.17重量份,0.005至0.15重量份,0.005至0.13重量份,0.005至0.1重量份,0.005至0.08重量份,0.005至0.06重量份,0.005至0.04重量份,0.01至0.2重量份,0.01至0.19重量份,0.01至0.17重量份,0.01至0.15重量份,0.01至0.13重量份,0.01至0.1重量份,0.01至0.08重量份,0.01至0.06重量份,0.02至0.1重量份,0.04至0.1重量份,0.04至0.08重量份,0.05至0.2重量份,0.05至0.15重量份,0.05至0.07重量份,0.1至0.2重量份,0.11至0.19重量份,0.11至0.17重量份,0.13至0.19重量份,0.13至0.17重量份,或0.14至0.16重量份,但經酶催化改質的甜菊萃之含量係非受此限。 In the present disclosure, based on 100 parts by weight of the solid content of the prosulose, the content of the stevia extract modified by the enzyme may be 0.001 to 1 part by weight, 0.001 to 0.5 part by weight, and 0.001 to 0.2 part by weight. 0.001 to 0.19 parts by weight, 0.001 to 0.17 parts by weight, 0.001 to 0.15 parts by weight, 0.002 to 1 part by weight, 0.002 to 0.5 parts by weight, 0.002 to 0.2 parts by weight, 0.002 to 0.19 parts by weight, 0.002 to 0.17 parts by weight, 0.002 to 0.15 parts by weight, 0.002 to 0.13 parts by weight, 0.005 to 0.2 parts by weight, 0.005 to 0.19 parts by weight, 0.005 to 0.17 parts by weight, 0.005 to 0.15 parts by weight, 0.005 to 0.13 parts by weight, 0.005 to 0.1 parts by weight, 0.005 to 0.08 parts by weight Parts, 0.005 to 0.06 parts by weight, 0.005 to 0.04 parts by weight, 0.01 to 0.2 parts by weight, 0.01 to 0.19 parts by weight, 0.01 to 0.17 parts by weight, 0.01 to 0.15 parts by weight, 0.01 to 0.13 parts by weight, 0.01 to 0.1 parts by weight, 0.01 to 0.08 parts by weight, 0.01 to 0.06 parts by weight, 0.02 to 0.1 parts by weight, 0.04 to 0.1 parts by weight, 0.04 to 0.08 parts by weight, 0.05 to 0.2 parts by weight, 0.05 to 0.15 parts by weight, 0.05 to 0.07 parts by weight, 0.1 to 0.2 parts by weight, 0.11 to 0.19 parts by weight, 0.11 to 0.17 parts by weight, 0.13 to 0.19 parts by weight, 0.13 to 0.17 parts by weight, or 0.14 to 0.16 parts by weight, but the content of the stevia extract modified by the enzyme is not limited.

於本揭示之用於製備甜味劑之組成物中,柑橘屬萃取物及經 酶催化改質的甜菊萃之含量可呈1:200至1:0.5之混合比,1:190至1:1之混合比,1:190至1:2之混合比,1:190至1:10之混合比,1:190至1:50之混合比,1:190至1:60之混合比,1:190至1:70之混合比,1:170至1:70之混合比,1:150至1:70之混合比,1:130至1:70之混合比,1:110至1:70之混合比,1:120至1:80之混合比,1:150至1:2之混合比,1:130至1:2之混合比,1:110至1:2之混合比,1:90至1:2之混合比,1:80至1:2之混合比,1:70至1:2之混合比,1:60至1:2之混合比,1:50至1:2之混合比,1:50至1:3之混合比,1:50至1:4之混合比,1:50至1:5之混合比,1:50至1:6之混合比,1:40至1:2之混合比,1:40至1:3之混合比,1:40至1:4之混合比,1:40至1:5之混合比,1:40至1:6之混合比,1:30至1:5之混合比,1:25至1:5之混合比,1:20至1:5之混合比,1:15至1:5之混合比,1:12至1:5之混合比,1:10至1:6之混合比,1:8至1:6之混合比,或1:7.5至1:6之混合比,但混合比並非受此所限。 In the composition for preparing a sweetener disclosed herein, the content of citrus extract and enzyme-catalyzed stevia extract may be in a mixing ratio of 1: 200 to 1: 0.5, 1: 190 to 1: 1 Mixing ratio, mixing ratio of 1: 190 to 1: 2, mixing ratio of 1: 190 to 1:10, mixing ratio of 1: 190 to 1:50, mixing ratio of 1: 190 to 1:60, 1: Mixing ratio of 190 to 1:70, mixing ratio of 1: 170 to 1:70, mixing ratio of 1: 150 to 1:70, mixing ratio of 1: 130 to 1:70, and ratio of 1: 110 to 1:70 Mixing ratio, mixing ratio of 1: 120 to 1:80, mixing ratio of 1: 150 to 1: 2, mixing ratio of 1: 130 to 1: 2, mixing ratio of 1: 110 to 1: 2, 1:90 Mixing ratio to 1: 2, mixing ratio of 1:80 to 1: 2, mixing ratio of 1:70 to 1: 2, mixing ratio of 1:60 to 1: 2, mixing ratio of 1:50 to 1: 2 Ratio, mixing ratio of 1:50 to 1: 3, mixing ratio of 1:50 to 1: 4, mixing ratio of 1:50 to 1: 5, mixing ratio of 1:50 to 1: 6, 1:40 to Mixing ratio of 1: 2, mixing ratio of 1:40 to 1: 3, mixing ratio of 1:40 to 1: 4, mixing ratio of 1:40 to 1: 5, mixing ratio of 1:40 to 1: 6 , 1:30 to 1 Mixing ratio of 5, mixing ratio of 1:25 to 1: 5, mixing ratio of 1:20 to 1: 5, mixing ratio of 1:15 to 1: 5, mixing ratio of 1:12 to 1: 5, 1 : 10 to 1: 6 mixing ratio, 1: 8 to 1: 6 mixing ratio, or 1: 7.5 to 1: 6 mixing ratio, but the mixing ratio is not limited by this.

於本揭示之用於製備甜味劑之組成物中,柚皮苷及經酶催化改質的甜菊萃之含量可呈0.001:200至0.001:0.5之混合比,0.001:190至0.001:1之混合比,0.001:190至0.001:2之混合比,0.001:190至0.001:10之混合比,0.001:190至0.001:50之混合比,0.001:190至0.001:60之混合比,0.001:190至0.001:70之混合比,0.001:170至0.001:70之混合比,0.001:150至0.001:70之混合比,0.001:130至0.001:70之混合比,0.001:110至0.001:70之混合比,0.001:120至0.001:80之混合比,0.001:150至0.001:2之混合比,0.001:130至0.001:2之混合比,0.001:110至0.001:2之混合比,0.001:90至0.001:2之混合比,0.001:80至0.001:2之混合比,0.001:70至0.001:2之混合比,0.001:60至0.001:2之混合比,0.001:50至0.001:2之混合 比,0.001:50至0.001:3之混合比,0.001:50至0.001:4之混合比,0.001:50至0.001:5之混合比,0.001:50至0.001:6之混合比,0.001:40至0.001:2之混合比,0.001:40至0.001:3之混合比,0.001:40至0.001:4之混合比,0.001:40至0.001:5之混合比,0.001:40至0.001:6之混合比,0.001:30至0.001:5之混合比,0.001:25至0.001:5之混合比,0.001:20至0.001:5之混合比,0.001:15至0.001:5之混合比,0.001:12至0.001:5之混合比,0.001:10至0.001:6之混合比,0.001:8至0.001:6之混合比,或0.001:7.5至0.001:6之混合比,但混合比並非受此所限。 In the composition for preparing a sweetener disclosed in the present disclosure, the content of naringin and stevia extract modified by an enzyme may be 0.001: 200 to 0.001: 0.5, and 0.001: 190 to 0.001: 1. Mixing ratio, 0.001: 190 to 0.001: 2, 0.001: 190 to 0.001: 10, 0.001: 190 to 0.001: 50, 0.001: 190 to 0.001: 60, 0.001: 190 To 0.001: 70, 0.001: 170 to 0.001: 70, 0.001: 150 to 0.001: 70, 0.001: 130 to 0.001: 70, 0.001: 110 to 0.001: 70 Ratio, 0.001: 120 to 0.001: 80 mixing ratio, 0.001: 150 to 0.001: 2 mixing ratio, 0.001: 130 to 0.001: 2 mixing ratio, 0.001: 110 to 0.001: 2 mixing ratio, 0.001: 90 to Mixing ratio of 0.001: 2, mixing ratio of 0.001: 80 to 0.001: 2, mixing ratio of 0.001: 70 to 0.001: 2, mixing ratio of 0.001: 60 to 0.001: 2, mixing ratio of 0.001: 50 to 0.001: 2 , 0.001: 50 to 0.001: 3 mixing ratio, 0.001: 50 to 0.001: 4 mixing ratio, 0.001: 50 to 0.001: 5 mixing ratio, 0.001: 50 Mixing ratio of 0.001: 6, mixing ratio of 0.001: 40 to 0.001: 2, mixing ratio of 0.001: 40 to 0.001: 3, mixing ratio of 0.001: 40 to 0.001: 4, mixing ratio of 0.001: 40 to 0.001: 5 , 0.001: 40 to 0.001: 6 mixing ratio, 0.001: 30 to 0.001: 5 mixing ratio, 0.001: 25 to 0.001: 5 mixing ratio, 0.001: 20 to 0.001: 5 mixing ratio, 0.001: 15 to 0.001 : 5 mixing ratio, 0.001: 12 to 0.001: 5 mixing ratio, 0.001: 10 to 0.001: 6 mixing ratio, 0.001: 8 to 0.001: 6 mixing ratio, or 0.001: 7.5 to 0.001: 6 mixing ratio , But the mixing ratio is not limited by this.

本揭示之用於製備甜味劑之組成物可進一步包含糊精、澱粉、安定劑、酸度調節劑、精鹽、二氧化矽、矯味劑、著色添加劑、或水。安定劑係指欲添加用於防止食品的物理或化學變化目的之材料。此外,除了上述者之外,本揭示之用於製備甜味劑之組成物可進一步包含常被使用來製備甜味劑的已知材料。 The composition for preparing a sweetener of the present disclosure may further include dextrin, starch, stabilizer, acidity regulator, refined salt, silica, flavoring agent, coloring additive, or water. Stabilizers are materials intended to be added for the purpose of preventing physical or chemical changes in food. Further, in addition to the above, the composition for preparing a sweetener of the present disclosure may further include a known material that is often used to prepare a sweetener.

安定劑之特定實施例可包括阿拉伯膠、瓊脂、明膠、赤槐豆膠、瓜爾膠、黃膠、褐藻酸鈉、果膠、CMC、酪蛋白鈉、白蛋白、磷酸鹽、檸檬酸鹽、羥丙基甲基纖維素等,但非受此所限。 Specific examples of stabilizers may include acacia, agar, gelatin, locust bean gum, guar gum, yellow gum, sodium alginate, pectin, CMC, sodium caseinate, albumin, phosphate, citrate, Hydroxypropyl methylcellulose and the like are not limited thereto.

本揭示之另一態樣提供一種製備用於製備甜味劑之組成物的方法或一種製備甜味劑的方法,其包含混合柚皮苷、經酶催化改質的甜菊萃、及涵括阿洛酮糖的醣之步驟。 Another aspect of the present disclosure provides a method for preparing a composition for preparing a sweetener or a method for preparing a sweetener, comprising mixing naringin, enzymatically modified stevia extract, and containing Lactose sugar steps.

本揭示之又另一態樣提供一種包含用於製備甜味劑之組成物的甜味劑。 Yet another aspect of the present disclosure provides a sweetener comprising a composition for preparing a sweetener.

本揭示之甜味劑可以是經由上述製備甜味劑的方法製備 者,以及可以是於其中由阿洛酮糖所產生的辛辣味或苦味減少者。 The sweetener of the present disclosure may be one prepared by the method for preparing a sweetener as described above, and may be one in which the pungent or bitter taste produced by psicose is reduced.

本揭示之又另一態樣提供一種用於改善辛辣味或苦味之組成物,其包含柚皮苷、經酶催化改質的甜菊萃、及涵括阿洛酮糖的醣。本揭示之又另一態樣提供一種用於改善辛辣味或苦味之方法,其包含混合柚皮苷、經酶催化改質的甜菊萃、及涵括阿洛酮糖的醣之步驟。 Yet another aspect of the present disclosure is to provide a composition for improving a spicy or bitter taste, which comprises naringin, an enzymatically modified stevia extract, and a sugar containing alloxulose. Yet another aspect of the present disclosure is to provide a method for improving a spicy or bitter taste, comprising the steps of mixing naringin, an enzymatically modified stevia extract, and a sugar containing psicose.

阿洛酮糖、醣、柚皮苷、經酶催化改質的甜菊萃、辛辣味、及苦味係如前文描述。 Alloxulose, sugar, naringin, enzymatically modified stevia extract, spicy flavor, and bitterness are as described above.

當本揭示之用於製備甜味劑之組成物或甜味劑被使用於食品組成物時,用於製備甜味劑之組成物或甜味劑可就此添加,或可連同其它食品組成物或成分一起使用,且可根據習知方法妥適地使用。考慮食物的口味及風味、使用目的、健康等,可妥善決定活性成分之混合量。食品組成物之實施例可包括各種醬汁、番茄醬、調味汁、飲料、茶、能量飲品、酒精飲料、綜合維生素、湯、乳製品、糖果、巧克力等,但非受此所限。此外,可自由地使用任何組成物而無限制,只要其能藉涵括用於製備甜味劑之組成物或甜味劑而製備食品即可。 When the composition for sweetener preparation or the sweetener of the present disclosure is used in a food composition, the composition or sweetener used for the preparation of the sweetener may be added thereto or may be used in conjunction with other food compositions or The ingredients are used together and can be appropriately used according to a conventional method. Considering the taste and flavor of the food, the purpose of use, health, etc., the amount of active ingredients can be properly determined. Examples of the food composition may include, but are not limited to, various sauces, ketchup, sauces, beverages, tea, energy drinks, alcoholic beverages, multivitamins, soups, dairy products, candy, chocolate, and the like. In addition, any composition can be freely used without limitation as long as it can prepare a food by including a composition or a sweetener for preparing a sweetener.

[進行本發明之模式]     [Mode for Carrying Out the Invention]    

後文中,將使用隨附之具體實施例以細節描述本揭示。然而,本文中揭示的具體實施例係僅供例示目的,而不應解譯為限制本揭示之範圍。 Hereinafter, the present disclosure will be described in detail using the accompanying specific embodiments. However, the specific embodiments disclosed herein are for illustrative purposes only and should not be interpreted as limiting the scope of the disclosure.

實施例1:包含液體阿洛酮糖之混合甜味劑組成物之製備Example 1: Preparation of a Mixed Sweetener Composition Containing Liquid Pulose

液體阿洛酮糖(希杰製糖(CJ CheilJedang);固體含量71±1%,阿洛酮糖(95%或以上)呈乾固體),柑橘屬萃取物(奎納拔 (Quinabra);粉末;柚皮苷(0.51重量%)、抗壞血酸(5.99重量%)、多酚(3.2重量%至5.7重量%)呈乾固體),及經酶催化改質的甜菊萃(戴皮恩(Daepyung);粉末;甜菊萃醇糖苷(90%或以上)呈乾固體),以下表1中顯示的混合比,使用電子秤稱重。為了製備柑橘屬萃取物及經酶催化改質的甜菊萃,萃取自柑橘屬植物及甜菊萃植物各自的萃取物藉純化及/或酶催化處理加工,然後,所得產物各自接受噴乾而獲得呈粉末形式的柑橘屬萃取物及經酶催化改質的甜菊萃。已稱重之液體阿洛酮糖於82℃水浴中加熱,及含有已稱重之柚皮苷的經酶催化改質的甜菊萃及柑橘屬萃取物徐緩加至其中,使得混合物可被均勻混合而不會凝聚。此等處理程序經重複,以製備比較例1及實驗例1至25的甜味劑糖漿。其後,將純水添加至如此製備的甜味劑糖漿中,以製備具有10%濃度的水性溶液,及辛辣味與甜味品質喜好係透過感官知覺試驗測定。 Liquid alloxulose (CJ CheilJedang; solid content 71 ± 1%, alloxulose (95% or above) is a dry solid), citrus extract (Quinabra; powder; Naringin (0.51% by weight), ascorbic acid (5.99% by weight), polyphenols (3.2% to 5.7% by weight) as a dry solid), and enzymatically modified stevia extract (Daepyung; powder ; Stevia extract glycosides (90% or more are dry solids), the mixing ratios shown in Table 1 below are weighed using an electronic scale. In order to prepare citrus extracts and enzyme-catalyzed stevia extracts, extracts extracted from citrus plants and stevia extract plants were purified and / or enzymatically treated, and then the resulting products were spray-dried to obtain Citrus extract in powder form and stevia extract modified by enzyme. The weighed liquid allosene is heated in a 82 ° C water bath, and the enzymatically modified stevia extract and citrus extract containing the weighed naringin are slowly added to it, so that the mixture can be uniformly mixed. Without condensing. These processing procedures were repeated to prepare the sweetener syrups of Comparative Example 1 and Experimental Examples 1 to 25. Thereafter, pure water was added to the sweetener syrup thus prepared to prepare an aqueous solution having a concentration of 10%, and the spicy and sweet taste preferences were determined through a sensory perception test.

實施例2:包含液體阿洛酮糖的混合甜味劑組成物之感官知覺評估Example 2: Sensory perception evaluation of a mixed sweetener composition containing liquid psicose

進行於實施例1中製備的各種混合甜味劑組成物的感官知覺評估。特定言之,感官知覺評估係採用5分量表,於30位或以上的評審員進 行。使用比較例作基礎,針對各個屬性之分數係基於T-試驗分析各個實驗例之結果後,以統計上顯著差異(p<0.05)顯示,及其結果係顯示於表2。 The sensory perception evaluation of each mixed sweetener composition prepared in Example 1 was performed. In particular, sensory perception assessment is performed on a 5-point scale with 30 or more reviewers. Using comparative examples as the basis, the scores for each attribute are shown as statistically significant differences (p <0.05) after analyzing the results of each experimental example based on the T-test, and the results are shown in Table 2.

(最強的辛辣味及苦味:5分;最弱的辛辣味及苦味:1分/最高甜味品質喜好:5分;最低甜味品質喜好:1分) (The strongest spicy and bitter taste: 5 points; the weakest spicy and bitter taste: 1 point / highest sweetness quality preference: 5 points; lowest sweetness quality preference: 1 point)

結果,不像不涵括柚皮苷及經酶催化改質的甜菊萃的比較例 1之組成物、及只涵括經酶催化改質的甜菊萃的比較例2至6之組成物,於涵括柚皮苷及經酶催化改質的甜菊萃的實驗例1至15的全部混合甜味劑組成物中,辛辣味及苦味減少,及阿洛酮糖的甜味品質喜好改善。 As a result, unlike the composition of Comparative Example 1 which did not include naringin and the enzyme-catalyzed stevia extract, and the composition of Comparative Examples 2 to 6 which only included the enzyme-catalyzed stevia extract, in In all the mixed sweetener compositions of Experimental Examples 1 to 15 including naringin and enzymatically modified stevia extract, the spicy and bitter tastes were reduced, and the sweet taste quality of the psicose was improved.

特別,使用於實驗例7至10中之經酶催化改質的甜菊萃及柚皮苷,於其中經酶催化改質的甜菊萃之含量為0.6重量份,及柚皮苷之含量為0.002至0.010重量份,係最有效地用於減少辛辣味及苦味,因而此等實驗例比較比較例顯示了顯著效果。據此,也確證於實驗例7至10中之總體甜味品質喜好也是最優異;及於實驗例7至10的組成比中,液體阿洛酮糖用於改善口感品質的效果顯示為顯著優異(比較實驗例7至10及比較例4之p值,及比較比較例8至10之p值也小於或等於0.05)。 In particular, the enzyme-catalyzed stevia extract and naringin used in Experimental Examples 7 to 10 had an enzyme-catalyzed stevia extract content of 0.6 parts by weight and a naringin content of 0.002 to 0.010 parts by weight is the most effective for reducing the spiciness and bitterness, so these experimental examples and comparative examples show significant effects. Accordingly, it was also confirmed that the overall sweetness quality preferences in Experimental Examples 7 to 10 were also the most excellent; and in the composition ratios of Experimental Examples 7 to 10, the effect of liquid allosexose for improving the taste quality was shown to be significantly excellent (The p-values of Comparative Experimental Examples 7 to 10 and Comparative Example 4, and the p-values of Comparative Comparative Examples 8 to 10 are also less than or equal to 0.05).

實施例3:包含粉狀阿洛酮糖的混合甜味劑組成物之製備Example 3: Preparation of a mixed sweetener composition containing powdered ptoseose

不似使用液體阿洛酮糖製備的實施例1之組成物,製備包含結晶阿洛酮糖的混合甜味劑組成物。 Instead of using the composition of Example 1 prepared using liquid psicose, a mixed sweetener composition containing crystalline psicose was prepared.

特定言之,結晶阿洛酮糖(希杰製糖(CJ CheilJedang);阿洛酮糖(98%或以上)呈乾固體),柑橘屬萃取物(奎納拔;粉末;柚皮苷(0.51重量%)、抗壞血酸(5.99重量%)、多酚(3.2%至5.7重量%)呈乾固體),及經酶催化改質的甜菊萃,以下表3中顯示的混合比稱重,及此等添加至零重力混合器(F20,西吉技術(SEJI TECH))中,及然後,以40rpm速率均質混合,以製備甜味劑粉末。已徹底混合的甜味劑粉末中之水分於恆溫烤爐內於75℃乾燥30分鐘。添加純水至甜味劑粉末以製備具有10%濃度的水性溶液,及透過感官知覺試驗測定辛辣味及甜味品質喜好。 In particular, crystalline allosene (CJ CheilJedang; allose (98% or more) as a dry solid), citrus extract (quinabate; powder; naringin (0.51 weight %), Ascorbic acid (5.99% by weight), polyphenols (3.2% to 5.7% by weight as dry solids), and stevia extract modified by enzymes, weighed at the mixing ratios shown in Table 3 below, and these additions Into a zero gravity mixer (F20, SEJI TECH), and then, homogeneously mix at a speed of 40 rpm to prepare a sweetener powder. The moisture in the thoroughly mixed sweetener powder is dried in a constant temperature oven at 75 ° C for 30 minutes. Pure water was added to the sweetener powder to prepare an aqueous solution having a concentration of 10%, and the spicy and sweet taste preferences were determined by a sensory perception test.

實施例4:包含粉狀阿洛酮糖之混合甜味劑組成物的感官知覺評估Example 4: Sensory perception evaluation of a mixed sweetener composition containing powdered psicose

進行於實施例3中製備的各種混合甜味劑組成物之感官知覺評估。特定言之,感官知覺評估係採用5分量表,於30位或以上的評審員進 行。使用比較例作基礎,針對各個屬性之分數係基於T-試驗分析各個實驗例之結果後,以統計上顯著差異(p<0.05)顯示,及其結果係顯示於表4。 The sensory perception evaluation of various mixed sweetener compositions prepared in Example 3 was performed. In particular, sensory perception assessment is performed on a 5-point scale with 30 or more reviewers. Using comparative examples as the basis, the scores for each attribute are shown as statistically significant differences (p <0.05) after analyzing the results of each experimental example based on the T-test, and the results are shown in Table 4.

(最強的辛辣味及苦味:5分;最弱的辛辣味及苦味:1分/最高甜味品質喜好:5分;最低甜味品質喜好:1分) (The strongest spicy and bitter taste: 5 points; the weakest spicy and bitter taste: 1 point / highest sweetness quality preference: 5 points; lowest sweetness quality preference: 1 point)

結果,不像不涵括柚皮苷及經酶催化改質的甜菊萃的比較例12之組成物、及只涵括經酶催化改質的甜菊萃的比較例13至17之組成物,於涵括柚皮苷及經酶催化改質的甜菊萃的實驗例26至29的全部混合甜味劑組成物中,辛辣味及苦味減少,及阿洛酮糖的甜味品質喜好改善。 As a result, unlike the composition of Comparative Example 12 which did not include naringin and enzymatically modified stevia extract, and the composition of Comparative Examples 13 to 17 which only contained enzymatically modified stevia extract, in In all of the mixed sweetener compositions of Experimental Examples 26 to 29 including naringin and enzymatically modified stevia extract, the pungent and bitter tastes were reduced, and the sweetness quality of psicose was improved.

特別,使用於實驗例33至35中之經酶催化改質的甜菊萃及柚皮苷,於其中經酶催化改質的甜菊萃之含量為0.15重量份,及柑橘屬萃取物之含量為0.0014至0.0020重量份(涵括於柑橘屬萃取物中之柚皮苷之含量為0.00000714至0.00001020重量份),係最有效地用於減少辛辣味及苦味,因此此等實驗例比較比較例顯示了顯著效果。據此,確證於實驗例33至35中之總體甜味品質喜好也是最優異;及於實驗例33至35的組成比中,結晶阿洛酮糖用於改善口感品質的效果顯示為顯著優異(比較實驗例33至35及比較例15之p值,及比較比較例20至22之p值係小於或等於0.05)。 In particular, the enzyme-catalyzed stevia extract and naringin used in Experimental Examples 33 to 35 had an enzyme-catalyzed stevia extract content of 0.15 parts by weight and a citrus extract content of 0.0014. To 0.0020 parts by weight (the content of naringin contained in the citrus extract is 0.00007714 to 0.0001020 parts by weight) is the most effective for reducing the spicy and bitter tastes. Therefore, the comparative examples of these experimental examples show significant effect. Based on this, it was confirmed that the overall sweetness quality preferences in Experimental Examples 33 to 35 were also the most excellent; and in the composition ratios of Experimental Examples 33 to 35, the effect of the crystalline alloxulose for improving the taste quality was shown to be significantly excellent ( The p-values of Comparative Experimental Examples 33 to 35 and Comparative Example 15 and the p-values of Comparative Comparative Examples 20 to 22 are less than or equal to 0.05).

因此,基於上述結果,發現當柚皮苷及經酶催化改質的甜菊萃係藉與液體阿洛酮糖或粉狀阿洛酮糖混合在一起使用時,不僅阿洛酮糖獨特的口感品質(例如,辛辣味及苦味)減少,同時阿洛酮糖的甜味品質喜好也增高,如此能進一步提高阿洛酮糖作為甜味劑的價值。 Therefore, based on the above results, it was found that when naringin and enzymatically modified stevia extract are used in combination with liquid allosexose or powdered alloseose, not only the unique taste quality of allosexose (E.g., spicy and bitter tastes) are reduced, and the sweetness quality preference of psicose is also increased, which can further increase the value of psicose as a sweetener.

雖然已經參考特定例示具體例描述本揭示,但熟諳本揭示相關技藝人士將瞭解,未背離本揭示之技術精髓或要義特點,可以其它特定形式具體實施本揭示。因此,於全部態樣中,上述具體例被視為例示性而非限制性。又復,本揭示之範圍係由隨附之申請專利範圍界定,而非由實 施方式界定,須瞭解衍生自本揭示及其相當物的意義及範圍之全部修改或變化皆係涵括於隨附之申請專利範圍的範疇內。 Although the present disclosure has been described with reference to specific examples, those skilled in the art will appreciate that the present disclosure may be embodied in other specific forms without departing from the technical spirit or essential characteristics of the disclosure. Therefore, in all aspects, the above specific examples are considered as illustrative and not restrictive. Again, the scope of this disclosure is defined by the scope of the accompanying patent application, not by the implementation. It is necessary to understand that all modifications or changes derived from the meaning and scope of this disclosure and equivalents are included in the accompanying Within the scope of patent application.

Claims (15)

一種用於製備甜味劑之組成物,包含柚皮苷、經酶催化改質之甜菊萃、及涵括阿洛酮糖之醣。     A composition for preparing a sweetener, comprising naringin, an enzymatically modified stevia extract, and a sugar containing alloxulose.     如申請專利範圍第1項所述之組成物,其中,該柚皮苷於該組成物中之含量係使得柚皮苷的苦味不被表現。     The composition according to item 1 of the scope of patent application, wherein the content of the naringin in the composition is such that the bitterness of naringin is not expressed.     如申請專利範圍第1項所述之組成物,其中,該組成物具有改善的口感品質。     The composition according to item 1 of the scope of patent application, wherein the composition has improved taste quality.     如申請專利範圍第3項所述之組成物,其中,該口感品質為因阿洛酮糖所致的辛辣味或苦味。     The composition according to item 3 of the scope of patent application, wherein the taste quality is a spicy or bitter taste caused by psicose.     如申請專利範圍第1項所述之組成物,其中,比較不包含柚皮苷及經酶催化改質的甜菊萃之用於製備甜味劑之組成物,該組成物具有改善的甜味品質。     The composition according to item 1 of the scope of the patent application, wherein the composition for preparing a sweetener which does not contain naringin and stevia extract which is enzymatically modified is compared, and the composition has improved sweetness quality .     如申請專利範圍第1項所述之組成物,其中,以該阿洛酮糖之100重量份固體含量為基準計,該柚皮苷之含量為10x10 -5至10x10 -6重量份。 The composition according to item 1 of the scope of patent application, wherein the content of the naringin is 10x10 -5 to 10x10 -6 parts by weight based on 100 parts by weight of the solid content of the alloseneose. 如申請專利範圍第1項所述之組成物,其中,以該阿洛酮糖之100重量份固體含量為基準,該經酶催化改質之甜菊萃之含量為0.001至1重量份。     The composition according to item 1 of the scope of the patent application, wherein the content of the enzymatically modified stevia extract is 0.001 to 1 part by weight based on 100 parts by weight of the solid content of the allosene.     如申請專利範圍第1項所述之組成物,其中,該柚皮苷及該經酶催化改質之甜菊萃係以0.001:190至0.001:2之混合比而被包含。     The composition according to item 1 of the patent application scope, wherein the naringin and the enzyme-catalyzed stevia extract are contained in a mixing ratio of 0.001: 190 to 0.001: 2.     如申請專利範圍第1項所述之組成物,其中以該用於製備甜味劑之組成物之100重量份總固體含量為基準計,該涵括阿洛酮糖之醣之含 量為80至99.99重量份。     The composition as described in item 1 of the scope of the patent application, wherein the content of the sugar including the allosexose is 80 to 100 parts by weight based on the total solid content of the composition used to prepare the sweetener. 99.99 parts by weight.     如申請專利範圍第1項所述之組成物,其中該組成物係呈液體或粉末形式。     The composition according to item 1 of the scope of patent application, wherein the composition is in liquid or powder form.     一種製備用於製備甜味劑之組成物之方法,包含混合柚皮苷、經酶催化改質之甜菊萃、及涵括阿洛酮糖之醣之步驟。     A method for preparing a composition for preparing a sweetener, comprising the steps of mixing naringin, an enzymatically modified stevia extract, and a sugar containing allosulose.     一種用於製備甜味劑之方法,包含混合柚皮苷、經酶催化改質之甜菊萃、及涵括阿洛酮糖之醣之步驟。     A method for preparing a sweetener, comprising the steps of mixing naringin, an enzymatically modified stevia extract, and a sugar containing alloxulose.     一種甜味劑,包含如申請專利範圍第1項所述之用於製備甜味劑之組成物。     A sweetener comprising the composition for preparing a sweetener as described in item 1 of the scope of patent application.     一種用於改善辛辣味或苦味之組成物,包含柚皮苷、經酶催化改質的甜菊萃、及涵括阿洛酮糖之醣。     A composition for improving the spicy or bitter taste, comprising naringin, an enzymatically modified stevia extract, and a sugar containing allosulose.     一種用於改善辛辣味或苦味之方法,包含混合柚皮苷、經酶催化改質之甜菊萃、及涵括阿洛酮糖之醣之步驟。     A method for improving a spicy or bitter taste, comprising the steps of mixing naringin, an enzymatically modified stevia extract, and a sugar containing alloxulose.    
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