TW201907918A - Medical use of 2-trifluoromethylbenzenesulfonamide derivatives - Google Patents

Abstract

The invention relates to the field of medicines related to hyperuricemia and gout. In particular, the present invention relates to a 2-trifluoromethyl-benzene sulfonamides derivative used as a URAT1 inhibitor, and particularly used as a therapeutic agent for a disorder associated with abnormal uric acid concentration. The 2-trifluoromethyl-benzene sulfonamides derivative of the present invention is a compound represented by the formula (I), wherein R1 is selected from the group consisting of H, F, Cl, Br, CN, nitro, CH2F, CHF2, CF3, NH2, OH, C1-C6 alkyl, C3-C6 cycloalkyl or C1-C6 alkoxy; X is S or O.

Description

Medical use of 2-trifluoromethylbenzenesulfonamide derivatives

The invention relates to the field of medicine related to treating hyperuricemia and gout. Specifically, it relates to a medical use of 2-trifluoromethylbenzenesulfonamide derivatives.

Gout is a chronic metabolic disease characterized by hyperuricemia and pain caused by the deposition of uric acid monosodium salt (MSU) in joints and other parts. The main causes are disorders of purine metabolism and / or uric acid excretion disorders. It is estimated that there are currently more than 20 million gout patients worldwide. The drugs currently used to treat gout are very limited: 1) Emergency medicines: colchicine tablets, non-steroidal anti-inflammatory drugs (NSAIDs) and glucocorticoids, used to relieve pain; 2) inhibition of uric acid production: allopurinol, side effects Febuxostat is ineffective in some patients; 3) Drugs that promote uric acid excretion: benzbromarone, etc., are prone to liver damage and are not suitable for patients with renal insufficiency; at the same time, these types of drugs have varying degrees of toxic and side effects.

On the other hand, with the improvement of national living standards, the extension of life expectancy, changes in diet structure (increased nucleoprotein-rich foods), the increase in obese people, and the increased emphasis on this disease, etc., gout is no longer The prevalence of rare diseases among Chinese people is about 15 to 30 times higher than 15 years ago. Therefore, the market urgently needs the emergence of new drugs for the treatment of gout.

URAT1 target inhibitors, namely urate transporter enzyme inhibitors, reduce the reabsorption of uric acid in the kidneys, and promote excretion, thereby reducing uric acid content. At present, many compounds for treating hyperuricemia and gout are in clinical trials and marketing phases. Among them, AstraZeneca's lesinurad has been successfully listed as a URAT1 inhibitor, and the company's new generation of URAT1 inhibitor RDEA-3170 has also entered clinical phase two. Currently published URAT1 inhibitor patents include WO2011046800, WO2011159839, WO2014183555A1, etc., but not many have actually entered the clinic. In order to achieve better therapeutic effect and better meet market demand, we hope to develop a new generation of highly effective and low toxicity inhibitors targeting URAT1 targets.

Currently JP02001483A discloses a series of 2-trifluoromethylbenzenesulfonamide derivatives for agricultural fungicides, but the use in the preparation of drugs that inhibit URAT1, or in the preparation of treatment and / or prevention of abnormal uric acid concentration as The use of medicines with characteristic disorders has not been reported.

The present invention provides the use of 2-trifluoromethylbenzenesulfonamide derivatives or pharmaceutically acceptable salts thereof, or pharmaceutical compositions in the preparation of drugs that inhibit URAT1, or in the preparation of treatment and / or prevention of uric acid For use in medicines for disorders characterized by abnormal concentration, the 2-trifluoromethylbenzenesulfonamide derivative has the structure shown in formula (I): Formula (I)

Wherein R _{1 is} selected from H, F, Cl, Br, CN, nitro, CH ₂ F, CHF ₂ , CF ₃ , NH ₂ , OH, C1-C6 alkyl, C3-C6 cycloalkyl or C1-C6 Alkoxy; X is S or O.

In a further preferred embodiment of the present invention, wherein R _{1 is} selected from H, Cl, Br, C1-C6 alkyl, C3-C6 cycloalkyl or C1-C6 alkoxy; more preferably, R ₁ It is selected from H, Cl, Br, methyl or isobutoxy.

In a further preferred embodiment of the present invention, wherein the compound of formula (I) may preferably be the following compound

A preferred embodiment of the present invention, wherein the disorder characterized by abnormal uric acid concentration is selected from gout, recurrent gout attack, gouty arthritis, hyperuricemia, hypertension, cardiovascular disease, coronary heart disease, lyl-naphthalene Syndrome, Kay-Sairy syndrome, kidney disease, kidney stones, kidney failure, arthritis, urolithiasis, lead poisoning, hyperparathyroidism, psoriasis, sarcoidosis or hypoxanthine-guanine Phosphoribosyl transferase deficiency, etc., preferably gout or hyperuricemia.

A preferred embodiment of the present invention, wherein the pharmaceutical composition contains a therapeutically effective amount of the compound represented by formula (I) of the present invention and a pharmaceutically acceptable salt thereof, and one or more pharmaceutically acceptable carriers, diluents or excipient. The 2-trifluoromethylbenzenesulfonamide derivative provided by the present invention exhibits an excellent serum uric acid lowering concentration, has a very good effect on the treatment of hyperuricemia and gout, and can be used to prepare and treat hyperuricemia and Gout medicine.

The definition of group in this application is as follows:

Alkyl: It is a saturated hydrocarbon group. It is a hydrocarbon group formed by removing one hydrogen atom from an alkane molecule.

Alkoxy: that is, the corresponding alcohol loses one hydrogen atom.

Cycloalkyl: It is a hydrocarbon group formed by removing one hydrogen atom from a cycloalkane molecule.

DMAP: 4-dimethylaminopyridine.

URAT1: Urate Transporter1, uric acid transporter 1.

LDA: lithium diisopropylamine.

THF: tetrahydrofuran.

Pharmaceutically acceptable salt: refers to a salt that is prepared so as not to harm or affect the human body in order to increase the solubility of the drug or to enhance the stability of the drug.

The column chromatography used is a silica gel column, 200 ~ 300 mesh.

The following examples illustrate that the present invention is not limited to the following embodiments.

The compounds of the present invention can be synthesized using the methods described below and known synthesis methods in the field of synthetic organic chemistry, or methods of variation known to those of ordinary skill in the art. Preferred methods include, but are not limited to, the methods described below; the reaction is carried out in a solvent that matches the reagents and materials used and the effective conversion; those with ordinary knowledge in the field of organic synthesis can understand the molecular performance The functionality should be consistent with the planned conversion; in order to obtain the desired compound of the present invention, sometimes a judgment is required to change the order of the synthesis steps or to select a specific process scheme.

The compound 2-amino-6-cyanobenzothiazole 1-A and the like in the examples were prepared by reference to Journal of Chemical Research, 2006, (12), 769-770.

The compound 2-amino-6-cyanobenzoxazole 2-A and the like in the examples were prepared by reference in Journal of Enzyme Inhibition and Medicinal Chemistry , (2011), 26 (6), 813-818.

Example 1

The compound 2-amino-6-cyanobenzothiazole 1-A (700 mg, 4 mmol) was dissolved in 7 ml of anhydrous THF, cooled to about -20 ° C, and the LDA / THF solution (4.8 mL, 1 mmol / ml, 1.2) was slowly added dropwise eq), continue to stir at this temperature for 0.5h after addition, add 2-trifluoromethylbenzene-1 sulfonyl chloride B (1.17g, 4.8mmol, 1.2eq), and continue to stir at 0 ° C after addition hour. Extracted with ethyl acetate, the crude product was separated and purified by silica gel column chromatography. The eluent was petroleum ether: ethyl acetate (V / V) = 1: 3, to obtain compound 1 (1.2g, yield 82%).

MS m / z (ESI): 384 [M + 1]; ¹ HNMR (400 MHz, d -DMSO) 13.9 (s, br, 1H), 8.27-8.35 (m, 2H), 7.83-7.97 (m, 4H ), 7.43 (d, J = 7.2Hz, 1H).

Example 2

The compound 2-amino-6-cyanobenzoxazole 2-A (636 mg, 4 mmol) was dissolved in 7 ml of anhydrous THF, cooled to about -20 ° C, and the LDA / THF solution (4.8 mL, 1 mmol / ml, 1.2eq), stirring was continued at this temperature for 0.5h after addition, 2-trifluoromethylbenzene-1sulfonyl chloride B (1.17g, 4.8mmol, 1.2eq) was added, and stirring was continued at 0 ° C after addition 2 hours. It was extracted with ethyl acetate, and the crude product was separated and purified by silica gel column chromatography. The eluent was petroleum ether: ethyl acetate (V / V) = 1: 3, to obtain compound 2 (1.15 g, yield 78%).

MS m / z (ESI): 368 [M + 1]; ¹ HNMR (400 MHz, d -DMSO) 8.34 (d, J = 8.0Hz, 1H), 8.10 (s, 1H), 7.96 (d, J = 8.0Hz, 1H), 7.82-7.91 (m, 2H), 7.74 (d, J = 8.0Hz, 1H), 7.43 (d, J = 8.0Hz, 1H).

Example 3

The compound 2-amino-4-methyl-6-cyanobenzothiazole 3-A (756 mg, 4 mmol) was dissolved in 8 ml of anhydrous THF, cooled to about -20 ° C, and the LDA / THF solution (4.8 mL, 1mmol / ml, 1.2eq), after stirring, continue to stir at this temperature for 0.5h, add 2-trifluoromethylbenzene-1 sulfonyl chloride B (1.17g, 4.8mmol, 1.2eq), after adding Stirring was continued for 2 hours at 0C. Extracted with ethyl acetate, the crude product was separated and purified by silica gel column chromatography. The eluent was petroleum ether: ethyl acetate (V / V) = 1: 2, to obtain compound 3 (1.25g, yield 79%).

MS m / z (ESI): 398 [M + 1]; ¹ HNMR (400 MHz, d -DMSO) 13.76 (s, br, 1H), 8.28 (d, J = 8.0Hz, 1H), 8.20 (s, 1H), 7.99 (d, J = 8.0Hz, 1H), 7.86-7.91 (m, 2H), 7.70 (s, 1H), 2.43 (s, 3H).

Example 4

The compound 2-amino-5-cyanobenzoxazole 4-A (636 mg, 4 mmol) was dissolved in 7 ml of anhydrous THF, cooled to about -20 ° C, and the LDA / THF solution (4.8 mL, 1 mmol / ml, 1.2eq), stirring was continued at this temperature for 0.5h after addition, 2-trifluoromethylbenzene-1sulfonyl chloride B (1.17g, 4.8mmol, 1.2eq) was added, and stirring was continued at 0 ° C after addition 2 hours. Extracted with ethyl acetate, the crude product was separated and purified by silica gel column chromatography. The eluent was petroleum ether: ethyl acetate (V / V) = 1: 3, to obtain compound 4 (1.07g, yield 73%).

MS m / z (ESI): 368 [M + 1]; ¹ HNMR (400 MHz, d -DMSO) 8.28 (d, J = 7.6Hz, 1H), 7.79 (d, J = 7.6Hz, 1H), 7.74 (t, J = 7.6Hz, 1H), 7.64 ((t, J = 7.6Hz, 1H), 7.48 (s, 1H), 7.31-7.37 (m, 2H).

Example 5

The compound 2-amino-6-bromo-4-cyanobenzothiazole 5-A (1.02g, 4mmol) was dissolved in 10ml of anhydrous THF, cooled to about -20 ° C, and the LDA / THF solution (4.8mL, 1mmol / ml, 1.2eq), after stirring, continue to stir at this temperature for 0.5h, add 2-trifluoromethylbenzene-1 sulfonyl chloride B (1.17g, 4.8mmol, 1.2eq), after adding Stirring was continued for 2 hours at 0C. Extracted with ethyl acetate, the crude product was separated and purified by silica gel column chromatography. The eluent was petroleum ether: ethyl acetate (V / V) = 1: 2, to obtain compound 5 (1.16g, yield 63%).

MS m / z (ESI): 462 [M + 1]; ¹ HNMR (400 MHz, d-MeOH) 8.27 (d, J = 7.2Hz, 1H), 7.96 (J = 2.0Hz, 1H), 7.78 (d , J = 7.2Hz, 1H), 7.67 (d, J = 2.0Hz, 1H), 7.60-7.66 (m, 2H).

Example 6

The compound 2-amino-6-isobutoxy-7-cyanobenzothiazole 6-A (988 mg, 4 mmol) was dissolved in 10 ml of anhydrous THF, cooled to about -20 ° C, and the LDA / THF solution (4.8 mL, 1mmol / ml, 1.2eq), after the addition is complete, continue to stir at this temperature for 0.5h, add 2-trifluoromethylbenzene-1 sulfonyl chloride B (1.17g, 4.8mmol, 1.2eq), after the addition Stirring was continued for 2 hours at 0 ° C. Extracted with ethyl acetate, the crude product was separated and purified by silica gel column chromatography. The eluent was petroleum ether: ethyl acetate (V / V) = 1: 3, to obtain compound 6 (1.38 g, yield 76%).

MS m / z (ESI): 456 [M + 1]; ¹ HNMR (400 MHz, CDCl3 ) 8.49 (d, J = 8.4Hz, 1H), 8.14 (J = 9.2Hz, 1H), 7.84 (d, J = 7.6Hz, 1H), 7.78-7.81 (m, 1H), 7.70-7.74 (m, 1H), 7.01 (d, J = 9.2Hz, 1H), 3.86 (d, J = 6.4Hz, 2H), 2.13 -2.20 (m, 1H), 1.06 (d, J = 6.8Hz, 6H).

Example 7

The compound 2-amino-7-bromo-5-cyanobenzoxazole 7-A (952mg, 4mmol) was dissolved in 10ml of anhydrous THF, cooled to about -20 ° C, and the LDA / THF solution (4.8mL, 1mmol / ml, 1.2eq), after stirring, continue to stir at this temperature for 0.5h, add 2-trifluoromethylbenzene-1 sulfonyl chloride B (1.17g, 4.8mmol, 1.2eq), after adding Stirring was continued for 2 hours at 0C. It was extracted with ethyl acetate, and the crude product was separated and purified by silica gel column chromatography. The eluent was petroleum ether: ethyl acetate (V / V) = 1: 2, to obtain compound 7 (1.09 g, yield 61%).

MS m / z (ESI): 446 [M + 1]; ¹ HNMR (400 MHz, d -DMSO) 8.37 (d, J = 7.6Hz, 1H), 7.98 (s, 1H), 7.95 (d, J = 7.6Hz, 1H), 7.85-7.92 (m, 1H), 7.81-7.83 (m, 1H), 7.70 (s, 1H).

Example 8

The compound 2-amino-4-bromo-6-cyanobenzoxazole 8-A (952mg, 4mmol) was dissolved in 10ml of anhydrous THF, cooled to about -20 ° C, and the LDA / THF solution (4.8mL, 1mmol / ml, 1.2eq), after stirring, continue to stir at this temperature for 0.5h, add 2-trifluoromethylbenzene-1 sulfonyl chloride B (1.17g, 4.8mmol, 1.2eq), after adding Stirring was continued for 2 hours at 0C. Extracted with ethyl acetate, the crude product was separated and purified by silica gel column chromatography. The eluent was petroleum ether: ethyl acetate (V / V) = 1: 2, to obtain compound 8 (1.14g, yield 64%).

MS m / z (ESI): 446 [M + 1]; ¹ HNMR (400 MHz, d -DMSO) 8.38 (d, J = 8.0Hz, 1H), 7.79 (d, J = 7.6Hz, 1H), 7.75 (d, J = 7.6Hz, 1H), 7.65-7.69 (m, 3H).

Example 9

The compound 2-amino-4-methyl-6-cyanobenzoxazole 9-A (692 mg, 4 mmol) was dissolved in 7 ml of anhydrous THF, cooled to about -20 ° C, and the LDA / THF solution (4.8 mL) was slowly added dropwise , 1mmol / ml, 1.2eq), after stirring, continue to stir at this temperature for 0.5h, add 2-trifluoromethylbenzene-1 sulfonyl chloride B (1.17g, 4.8mmol, 1.2eq), after adding The stirring was continued for 2 hours at 0 ° C. Extracted with ethyl acetate, the crude product was separated and purified by silica gel column chromatography, and the eluent was petroleum ether: ethyl acetate (V / V) = 1: 2, to obtain compound 9 (1.28g, yield 84%).

Example 10

The compound 2-amino-4-cyano-6-isobutoxybenzothiazole 10-A (988 mg, 4 mmol) was dissolved in 10 ml of anhydrous THF, cooled to about -20 ° C, and the LDA / THF solution (4.8 mL, 1mmol / ml, 1.2eq), after the addition is complete, continue to stir at this temperature for 0.5h, add 2-trifluoromethylbenzene-1 sulfonyl chloride B (1.17g, 4.8mmol, 1.2eq), after the addition Stirring was continued for 2 hours at 0 ° C. Extracted with ethyl acetate, the crude product was separated and purified by silica gel column chromatography. The eluent was petroleum ether: ethyl acetate (V / V) = 1: 3, to obtain compound 10 (1.42g, yield 78%).

MS m / z (ESI): 456 [M + 1]; ¹ HNMR (400 MHz, d -DMSO) 8.24 (d, J = 7.6Hz, 1H), 7.92 (d, J = 7.6Hz, 1H), 7.73 -7.85 (m, 2H), 7.72 (s, 1H), 7.35 (s, 1H), 3.77 (d, J = 6.4Hz, 2H), 1.96-2.03 (m, 1H), 0.97 (s, 3H), 0.95 (s, 3H).

Example 11

The compound 2-amino-6-cyano-5-isobutoxybenzoxazole 11-A (924 mg, 4 mmol) was dissolved in 10 ml of anhydrous THF, cooled to about -20 ° C, and the LDA / THF solution was slowly added dropwise ( 4.8mL, 1mmol / ml, 1.2eq), after the addition, continue to stir at this temperature for 0.5h, add 2-trifluoromethylbenzene-1 sulfonyl chloride B (1.17g, 4.8mmol, 1.2eq), add After that, stirring was continued at 0 ° C for 2 hours. It was extracted with ethyl acetate, and the crude product was separated and purified by silica gel column chromatography. The eluent was petroleum ether: ethyl acetate (V / V) = 1: 2, to obtain compound 11 (1.39 g, yield 79%).

MS m / z (ESI): 440 [M + 1]; ¹ HNMR (400 MHz, CDCl3) 8.22 (d, J = 7.6Hz, 1H), 8.07 (d, J = 7.6Hz, 1H), 7.95 (t , J = 7.6Hz, 1H), 7.84 (t, J = 7.6Hz, 1H), 7.31 (s, br, 1H), 7.08 (s, 1H), 6.81 (s, 1H), 3.84 (d, J = 6.0Hz, 2H), 2.14-2.21 (m, 1H), 1.08 (s, 3H), 1.06 (s, 3H).

Activity evaluation

In order to understand the uric acid lowering activity of the compounds of the present invention, the present invention designed a screening test for compounds that affect the absorption of uric acid on the target of human urate transporter 1 (hURAT1).

experiment procedure:

Uptake of ¹⁴ C-labeled uric acid in hURAT1 transfected cells:

Stable transfection of human URAT1 HEK-293T cells (ordered from ATCC), add polylysine-coated 96-well dishes at 6 × 10 ⁴ cells / well, 37 ° C, 5% CO ₂ , 100% relative humidity Incubate in an incubator; after at least 12h, spread the 96-well plate with pre-warmed HBSS buffer (8g / L NaCl, 0.4g / LKCl, 1g / glucose, 60mg / LKH ₂ PO ₄ , 47.5mg / L Na ₂ HPO ₄ , pH = 7.2) Wash 200 μl / well three times, and remove all the buffer from the tray for the last time; add 50 μL of Cl-free HBSS buffer to each well, which contains uric acid (0.1 μCi / well) and different concentrations The test compound was incubated at 37 ° C for 5 min; after 5 min, the incubation solution was discarded and 100 μl of pre-chilled Cl-free HBSS buffer was added to stop the absorption of uric acid; this buffer was washed three more times, and the tray was removed for the last time Add 50 μL / well of lysis buffer (purchased from Abcam, USA) to all buffers and shake for 10 min at 600 rpm; after shaking, take 45 μL of supernatant in another 96-well dish and add 150 μL / well to each well Well Ultima Gold ^™ XR scintillation cocktail (scintillation liquid, purchased from PERKIN ELMER) was shaken for 10 min at 600 rpm; read the value on a liquid scintillation / luminescence counter (MicroBeta2, purchased from PERKIN ELMER) and analyze the data.

test results:

IC ₅₀ , the half maximal inhibitory concentration, is used to measure the ability of drugs to induce apoptosis. The experiment measures the ability of the compound to inhibit the absorption of uric acid by HEK-293T cells transfected with hURAT1. The stronger the inhibitory ability , The lower the IC ₅₀ value. In this screening, it is based on samples (the reference substance benzbromarone (purchased from Aladdin with a purity greater than 99%) and compounds 1-11 of the present invention) at 10 different concentrations (ie 20 μM, 5 μM, 1.25 μM, 0.3125 μM, 0.07831 μM, 0.01953 μM, 0.00488 μM, 0.00122 μM, 0.00531 μM, 0.00008 μM) calculated based on the above test inhibition of the receptor.

The IC ₅₀ value of each compound is shown in the following table:

Conclusion: The 2-trifluoromethylbenzenesulfonamide derivatives of the present invention have a significant inhibitory effect on human urate transporter 1 (hURAT1), and can be used to treat disorders related to abnormal uric acid concentration such as hyperuricemia and gout .

no

no

Claims (5)

Use of a compound represented by formula (I) or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition containing the compound represented by formula (I) or a pharmaceutically acceptable salt thereof in the preparation of a drug that inhibits URAT1, Or use in the preparation of a medicament for the treatment and / or prevention of a disorder characterized by abnormal uric acid concentration: Formula (I) wherein R _{1 is} selected from H, F, Cl, Br, CN, nitro, CH ₂ F, CHF ₂ , CF ₃ , NH ₂ , OH, C1-C6 alkyl, C3-C6 cycloalkyl Or C1-C6 alkoxy; X is S or O. The use as described in item 1 of the patent application scope, wherein the disorder characterized by abnormal uric acid concentration is selected from gout, recurrent gout attack, gouty arthritis, hyperuricemia, hypertension, cardiovascular disease, coronary heart disease , Ley-Naphthalene Syndrome, Kay-Sairy Syndrome, Kidney Disease, Kidney Stones, Kidney Failure, Arthritis, Urolithiasis, Lead Poisoning, Hyperparathyroidism, Psoriasis, Sarcoidosis or Secondary Xanthine-guanine phosphoribosyl transferase deficiency, preferably gout or hyperuricemia. The use as described in item 1 of the patent application scope, wherein R _{1 is} selected from H, Cl, Br, C1-C6 alkyl, C3-C6 cycloalkyl or C1-C6 alkoxy; more preferably, R _{1 is} selected From H, Cl, Br, methyl or isobutoxy. The use as described in any of items 1 to 3 of the patent application scope, wherein the compound represented by formula (I) is selected from: , , , , , , , , , or . The use according to any one of items 1 to 3 of the patent application range, wherein the pharmaceutical composition contains a therapeutically effective amount of the compound represented by formula (I) and a pharmaceutically acceptable salt thereof, and one or more A pharmaceutically acceptable carrier, diluent or excipient.