TW201738560A - Biological detecting cartridge and flowing method of detected fluid thereof - Google Patents

Abstract

A biological detecting cartridge adapted to gather a detected fluid includes a collection port, a first flowing layer structure connected through the collection port and a second flowing layer structure connected through the first flowing layer structure. The first and the second flowing layer structures are disposed at different levels in the biological detecting cartridge. A flowing method of detected fluid of biological detecting cartridge is further provided.

Description

Biological detection cassette and method for detecting fluid flow thereof

The present invention relates to a method of detecting a cassette and a flow of the detection fluid thereof, and more particularly to a method of flowing a bioassay cassette and its detection fluid.

In general, in order to reduce the size of the bioassay cassette, a microchannel structure is employed in the bioassay cassette and the detection liquid flows in the microchannel structure by capillary action. However, the microchannel structure must be produced in a high-precision manner in the production process, which is difficult to produce and has a high defect rate.

In addition, when using a bioassay cassette for detection, it is generally necessary to provide a quantitative detection fluid (eg, blood) to mix with the agent in the bioassay cassette. More specifically, since the amount of the drug in the bioassay cassette is fixed, if the amount of the detection fluid that reacts with the drug in the bioassay cassette is too large or too small, an error occurs in the detected value.

In addition, whether the test fluid entering the bioassay cassette can be uniformly mixed with the drug in the bioassay cassette has a significant impact on the test results. If the test fluid in the bioassay cassette is not evenly mixed with the drug, accurate measurement values cannot be provided for the user's reference. Especially in the case of a micro-sample, since the amount of the detection fluid is too small, it is difficult to uniformly mix with the drug. Therefore, in order to avoid the above situation, it is generally necessary to wait for a long time to ensure that the micro-sample in the bioassay cassette is The two drugs are completely mixed evenly before the next biochemical test can be performed.

The invention provides a biological detection cassette, which is designed by the flow path structure in the biological detection cassette so that the detection fluid can flow in the flow path structure in the biological detection cassette by applying driving forces in different directions, thus, It can effectively reduce the need for fineness of manufacturing. In addition, by designing the flow channel structure in the bioassay cassette, the detection fluid reactive with the agent can be quantified, and the test fluid can be quickly mixed with the agent to provide stable test results.

The invention provides a method for detecting a flow of a detection fluid of a biological detection cassette, which applies a driving force of different directions to a detection fluid in a biological detection cassette at different time points, and allows a flow path structure of the detection fluid in the biological detection cassette It flows in and can effectively and quickly mix the test fluid with the agent to reduce the time required for mixing.

An embodiment of the present invention provides a biological detection cassette adapted to collect a detection fluid, the biological detection cassette comprising: a collection port; a first layer flow structure connected to the collection port; and a second layer circulation structure, and The first layer flow structure is in communication, wherein the first layer flow structure and the second layer flow structure are disposed on different planes within the biological detection cassette.

An embodiment of the present invention provides a method for detecting a fluid flow of a biometric cassette, comprising: providing a detection fluid to a biometric cartridge; and applying a first driving force corresponding to a first direction at a first time point The detection fluid in the bioassay cassette; and, at a second time point, applying a second driving force corresponding to a second direction to the detection fluid in the biometric cartridge, wherein the first direction is different from the second direction.

The above described features and advantages of the invention will be apparent from the following description.

1 is a perspective view of a biometric cartridge 100 according to an embodiment of the invention; and FIG. 2 is a top perspective view of the biometric cartridge 100 of FIG. In the present embodiment, the biometric cartridge 100 includes a collection port 110, a first layer flow structure 120, and a second layer flow structure 140, wherein the collection port 110 communicates with the first layer flow structure 120, and the first The layer flow structure 120 is in communication with the second layer flow structure 140, and the first layer flow structure 120 and the second layer flow structure 140 are disposed on different planes inside the biometric cassette 100.

In the present embodiment, the first layer flow structure 120 and the second layer flow structure 140 are disposed inside the biometric cassette 100, and the first layer flow structure 120 is disposed above the second layer flow structure 140, and the second layer The flow-through structure 140 is in communication. The first layer flow structure 120 and the second layer flow structure 140 partially overlap each other. As shown in FIG. 2, the first layer flow structure 120 inside the biometric cartridge 100 is indicated by a solid line, and the second layer flow structure 140 is indicated by a broken line.

In the embodiment of the present invention, since the first layer flow structure 120 and the second layer flow structure 140 are disposed on different planes inside the biological detection cassette 100, and the first layer flow structure 120 and the second layer flow structure 140 are between each other They can be partially overlapped, so the size of the biometric cartridge 100 can be effectively reduced.

Referring to FIG. 1 and FIG. 2 , the first layer flow structure 120 of the biometric cassette 100 of the present embodiment includes a first flow path 122 and a separation groove 160 . The first flow channel 122 is in communication with the collection port 110, and the first flow channel 122 is also in communication with the separation groove 160. The second layer flow structure 140 of the biometric cartridge 100 of the present embodiment includes at least one shunt structure 141. Each of the flow dividing structures 141 includes a certain amount of grooves 130, a second flow path 142, and a mixing tank group 150. The dosing tank 130 is in communication with the first flow path 122 of the first layer flow structure 120, and the dosing tank 130 is connected to the mixing trough group 150 through the second flow path 142.

In the embodiment of the present invention, when the detection fluid F (indicated in FIG. 4) enters the biometric cartridge 100 from the collection port 110, the corresponding direction can be applied to the detection fluid F in the biometric cartridge 100 at different time points. The driving force causes the detection fluid F to flow in the first layer flow-through structure 120 and the second layer flow-through structure 140 inside the bio-detection cassette 100.

Please refer to FIG. 3A and FIG. 3B . FIG. 3A and FIG. 3B are schematic diagrams of an embodiment of a detecting device 10 suitable for the biometric cassette 100 of the embodiment of the present invention, which respectively show biometric cards at different time points. The relationship between the crucible 100 and the detecting device 10. In this embodiment, the detection device 10 includes a rotating base 12. The rotating base 12 is rotatable along a first rotational axis 14. The biometric cartridge 100 can be placed on the rotating base 12 and rotatable relative to the rotating base 12 along a second rotational axis 16, wherein the first rotational axis 14 is not coaxial with the second rotational axis 16. In addition, the biometric cartridge 100 can also be rotated about the first rotational axis 14 by the rotation of the rotating base 12. In other words, the biometric cartridge 100 can revolve around the first axis of rotation 14 and can also rotate about the second axis of rotation 16.

In the detecting device 10 of this embodiment, after the biometric cartridge 100 is rotated to a corresponding angle along the second rotational axis 16 at different time points, the biometric cartridge 100 is rotated around the rotating base 12 A rotation axis 14 is rotated, so that a centrifugal force corresponding to the direction of the detection fluid F can be applied.

For example, assume that at the first time point, the biometric cartridge 100 is rotated along the second axis of rotation 16 to an angle as shown in FIG. 3A. In the angle of FIG. 3A, point A on the biometric cartridge 100 is an extension line that is lined between the first rotational axis 14 and the second rotational axis 16. At this time, when the biometric cartridge 100 is rotated about the first rotation axis 14 by the rotation of the rotation base 12, the detection fluid F in the biometric cartridge 100 is subjected to the centrifugal force in the direction of the A point, and further to the point A. The direction flows.

Next, assume that at the second time point, the biometric cartridge 100 is rotated along the second rotational axis 16 to an angle as shown in FIG. 3B. In the angle of FIG. 3B, point B on the biometric cartridge 100 is an extension line that is lined between the first rotational axis 14 and the second rotational axis 16. At this time, when the biometric cartridge 100 is rotated about the first rotation axis 14 by the rotation of the rotation base 12, the detection fluid F in the biometric cartridge 100 is subjected to the centrifugal force in the direction B, and further to B. Flow in the direction of the point.

Through the detecting device 10 of the above embodiment, the detecting fluid F in the biometric cartridge 100 can receive the driving force in the corresponding direction at different time points, and the first layer circulating structure of the detecting fluid F inside the biological detecting cassette 100 120 flows in the second layer flow structure 140. In the present invention, the detecting device 10 is not limited to the above embodiment. In the present invention, the detecting device 10 is mainly used to apply a driving force in a corresponding direction to the detecting fluid F in the biological detecting cassette at different time points. In another embodiment, the detecting device 10 can also move the biometric detection card proposed by the present invention in different directions at different time points to achieve the detection fluid F in the biometric detection card at different time points. The effect of applying a driving force in a corresponding direction is applied.

In the embodiment of the present invention, since the moving manner of the detecting fluid F in the biological detecting device 10 is moved to different positions in the biological detecting device 10 by the driving force (for example, centrifugal force), the inside of the biological detecting device 10 The flow path size has a low fineness limit, is convenient to manufacture, and has the advantages of good yield and low cost.

4 to 11 are schematic views showing the flow of the detection fluid F in the biometric cartridge 100 of the embodiment of the present invention. In FIGS. 4-6, and 8, the first layer flow structure 120 inside the bioassay cassette 100 is shown in solid lines, while the second layer flow structure 140 is shown in dashed lines. In FIGS. 9-11, the first layer flow structure 120 inside the biometric cassette 100 is shown in dashed lines, while the second layer flow structure 140 is shown in solid lines. The internal structure of the bioassay cassette 100 will be described in detail below, and the flow and mixing method of the detection fluid F in the bioassay cassette 100 will be described together.

As shown in FIGS. 4 and 5A, the detection fluid F enters the biometric cartridge 100 from the collection port 110, and then enters the first flow channel 122 that communicates with the collection port 110. After the detection fluid F enters the first flow path 122, the detection device 10 applies a driving force in a corresponding direction to the detection fluid F according to the extending direction of the first flow path 122, and causes the detection fluid F to flow along the first flow path 122.

In the present embodiment, the first flow path 122 includes a plurality of first branch portions 122a, 122b, 122c that are bent and connected. More specifically, the first flow path 122 includes three first branches 122a, 122b, 122c connected in series, wherein a bend is formed between each of the two connected first branches 122a, 122b, 122c. In order to sequentially flow the detection fluid F along the first flow path 122 through the first branch portions 122a, 122b, 122c, the detecting device 10 respectively detects the fluid F at different time points according to the extending direction of the first branch portions 122a, 122b, 122c. A driving force parallel to the extending direction of the first branch portions 122a, 122b, 122c is applied.

For example, as shown in FIG. 5A, when the detecting fluid F is to flow through the first branch portion 122a, the detecting device 10 applies a direction parallel to the extending direction of the first branch portion 122a according to the extending direction of the first branch portion 122a. The driving force D1 causes the detecting fluid F to flow through the first branch portion 122a and gather at the bend between the two first branch portions 122a and 122b as shown in Fig. 5B. Next, when the detection fluid F is to flow through the next first branch portion 122b, the detecting device 10 applies a driving force D2 parallel to the extending direction of the first branch portion 122b to the detecting fluid F according to the extending direction of the first branch portion 122b. The detecting fluid F flows through the first branch portion 122b and is gathered at a bend between the two first branch portions 122b and 122c as shown in FIG. And so on.

In the present embodiment, the first flow path 122 includes three first branches 122a, 122b, 122c connected in series, and the bent design existing between the first branch portions 122a, 122b, 122c of the series can be in the detection fluid F The driving force in different directions is applied to prevent the detecting fluid F from flowing back during the flow of the detecting fluid F in the first flow path 122. However, the number of the first branch is not limited thereto, and a person skilled in the art can design a different number of first branches according to actual needs. The method of detecting the flow of the fluid F in each of the first branches is similar to that described above and will not be described herein.

Referring to FIG. 6, the separation groove 160 of the first layer flow-through structure 120 is connected to a bend between the two first branches 122b, 122c, and the separation groove 160 extends in a direction parallel to one of the first branches 122b. The detecting fluid F flows through the two first branch portions 122b and 122c in sequence, and the extending direction of the separating groove 160 is parallel to the first branch portions 122b and 122c, and the first branch portion through which the fluid F flows first is detected. 122b. As shown in FIG. 5B, the separation groove 160 is connected to the bend between the two first branches 122b and 112c, and since the detection fluid F flows through the first branch 112b before flowing through the first branch 112c, the separation groove The extending direction of 160 is parallel to the first branch portion 122b.

In the present embodiment, the separation groove 160 of the first layer flow-through structure 120 serves to separate portions of the detection fluid F at different densities by continuously applying a driving force to the detection fluid F in a direction in which the separation groove 160 extends. Wherein, under the continuous application of the driving force, the first portion F1 having a larger density in the detecting fluid F flows into the separating groove 160, and the second portion F2 having the smaller density in the detecting fluid F is still located in the first flow path 122. As shown in Figure 6.

Taking the detection fluid F as blood F as an example, the blood F includes a mixed first portion having a larger density, that is, a blood cell F1; and a second portion having a smaller density, that is, plasma F2. Since the blood F flows by the driving force (for example, centrifugal force) in the biometric cartridge 100, in FIG. 5B, when the driving force D2 parallel to the extending direction of the first branch portion 122b is applied to the blood F, the blood F is caused to flow. After the first branch portion 122b is gathered at the bend between the two first branch portions 122b and 122c, the detecting device 10 continues to apply the driving force D2 toward the blood F to the direction in which the separation groove 160 extends, so that the blood cell F1 having a large density is provided. Moving into the separation tank 160, the plasma F2 having a smaller density moves to the first branch portion 122c of the first flow path 122, as shown in FIG. Of course, in other embodiments, if the biometric cartridge 100 does not need to separate the detection fluid from a component having a large density and a low density, the design of the separation groove 160 may be omitted.

In the present embodiment, the first layer flow-through structure 120 is connected to at least one of the plurality of first branch portions 122a, 122b, 122c of the first flow path 122 and the at least one shunt structure 141 of the second layer flow-through structure 140. In addition, the first branch portion 122c connected to the shunt structure 141 is also referred to as a connecting branch portion 122c. In other words, the plurality of first branches 122a, 122b, 122c of the first flow path 122 include a connection branch 122c that is coupled to the flow dividing structure 141. In the present embodiment, the connecting branch portion 122c has a corresponding structural design with the splitting structure 141, and will be described later in conjunction with the second layer circulating structure 140.

Referring to FIG. 1, the second layer flow structure 140 includes at least one shunt structure 141. Each of the flow dividing structures 141 includes a certain amount of grooves 130, a second flow path 142, and a mixing groove group 150. The dosing tank 130 communicates with the connecting branch portion 122c of the first flow path 122, the second flow path 142 communicates with the dosing tank 130, and the mixing trough group 150 communicates with the second flow path 142. In the present embodiment, the second layer flow structure 140 is located below the first layer flow structure 130, and the dosing groove 130 partially overlaps and communicates with the connection branch portion 122c of the first flow path 122.

Referring to FIG. 6, after the blood F passes through the separation tank 160 for separation of different densities, the plasma F2 having a smaller density is located in the first flow path 122, and is gathered at a bend between the first branch portion 122b and the connecting branch portion 122c. At the office. Next, a driving force D3 parallel to the extending direction of the connecting branch portion 122c is applied to the plasma F2. When the plasma F2 is in the process of being subjected to the driving force D3 and flowing in the connecting branch portion 122c, the plasma F2 is preferentially entered into the metering tank 130 of the second layer flow-through structure 140 by the structural design of the corresponding branching structure 141 on the connecting branch portion 122c. The details will be described later.

In another embodiment of the present invention, if the biometric cartridge 100 does not need to separate the detection fluid from the component having a large density and a small density, and the design of the separation groove 160 is omitted, the detection fluid F flows through the first branch portion 122b. After being gathered at the bend between the first branch portion 122b and the connecting branch portion 122c, the driving force D3 parallel to the extending direction of the connecting branch portion 122c may be applied to the detecting fluid F, and the corresponding shunting structure 141 on the connecting portion 122c may be connected. It is designed to preferentially access the detection fluid F into the metering tank 130 of the second layer flow-through structure 140.

Please refer to FIG. 2 and FIG. 6 to FIG. 8. FIG. 7 is a cross-sectional view of FIG. 6 along line A-A, and FIG. 8 is a partial enlarged view of FIG. The connection branch portion 122c has a reduction passage 123 and a communication hole 125 corresponding to each of the flow dividing structures 141. The connecting branch portion 122c communicates with the dosing groove 130 of the corresponding shunting structure 141 through the communication hole 125. The flow-through section width W3 of the communication hole 125 is larger than the flow-through section width W2 of the reduction passage 123, and the communication hole 125 is located on the entry side of the detection fluid F of the reduction passage 123. In other words, the detection fluid F flows first through the communication hole 125 and then flows through the reduction passage 123.

In the present embodiment, since the flow cross-sectional width W3 of the communication hole 125 is larger than the flow cross-sectional width W2 of the narrowing passage 123, the detection fluid F first flows to the communication hole 125 under the driving force D3 parallel to the extending direction of the connecting branch portion 122c. Flow into the dosing tank 130. When the dosing tank 130 is full, since the detection fluid F cannot flow into the dosing groove 130 again to the communication hole 125, the overflowed detection fluid F enters the reduction passage 123 under the driving force D3 and flows through the reduction passage 123.

Referring to FIG. 6, in the example in which the fluid F is detected as the blood F, the blood F is collected at a bend between the first branch portion 122b and the connecting branch portion 122c after separation of the different densities through the separation tank 160. F2 is applied with a driving force D3 parallel to the extending direction of the connecting branch portion 122c. Next, referring to FIG. 7 and FIG. 8, when the plasma F2 flows along the connecting branch portion 122c to the position corresponding to the shunting structure 141 (as shown in FIG. 8), the flow cross-sectional width W3 of the communicating hole 125 is larger than the circulation of the narrowing passage 123. The section width W2 is such that the plasma F2 first flows into the quantification tank 130 toward the communication hole 125 (as shown in Fig. 7). When the corresponding dosing tank 130 is full, the overflowed plasma F2 will flow through the narrowing passage 123 and continue to flow along the connecting branch 122c. If the biometric cartridge 100 has a plurality of diverting structures 141, the remaining plasma F2 will continue to flow along the connecting branch 122c to a position corresponding to the next shunting structure 141, and the above-described flow process is repeated.

In the present embodiment, the detection fluid F first flows into and fills the dosing groove 130 by the connection branch portion 122c corresponding to the design of the narrowing passage 123 of the dosing groove 130 and the different cross-sectional width of the communication hole 125, so that the detecting fluid entering the diverting structure 141 is made. F has a set amount. In the present embodiment, the biometric cartridge 100 has four shunting structures 141. Similarly, the connecting branch 122c has four narrowing passages 123 and four communicating holes 125, which respectively correspond to the quantifying slots 130 of the four diverting structures 141.

As can be seen from FIG. 6 to FIG. 9, by the design of the connecting portion 122c corresponding to the different cross-sectional widths of the narrowing passage 123 and the communicating hole 125 of the dosing groove 130, the order in which the plasma F2 flows tends to flow through the communicating hole 125 to the dosing groove 130 first. Further to the left, the channel 123 is narrowed. In other words, plasma F2 fills these dosing tanks 130 one by one from right to left. The dosing tank 130 ensures that the amount of plasma that flows into the mixing tank set 150 thereafter is between a set range to avoid detection errors due to excessive or too little plasma.

In addition, please return to FIG. 2 , in this embodiment, the biometric cartridge 100 further includes an overflow tank 170 connected to one end of the first flow channel 122 away from the collection port 110 . As shown in FIG. 9, after the plasma F2 is filled in the dosing tanks 130, the excess plasma F2 flows from the connecting branch portion 122c into the overflow tank 170. Furthermore, referring to FIG. 2, one end of the first flow path 122 away from the collection port 110 can also communicate with an exhaust hole 190 of the exposed biological detection cassette 100, so that the detection fluid F can flow smoothly in the first flow path 122. .

Referring to FIG. 9 again, in the present embodiment, the number of the shunt structures 141 is exemplified by four, but the number of the shunt structures 141 may vary with the number of detection items of the biometric cassette. The second flow passage 142 of each of the flow dividing structures 141 includes a plurality of second branch portions 142a, 142b that are bent and connected. In this embodiment, there is a bend between the two second branches 142a, 142b to avoid backflow of plasma F2. After the plasma F2 flows to the quantification tank 130, the detecting device 10 may first apply a driving force D4 parallel to the extending direction of the second branch portion 142a to the plasma F2, and cause the plasma F2 to flow through the second branch portion 142a, and then apply parallel to the first portion 142a. The driving force D5 in the extending direction of the two branch portions 142b causes the plasma F2 to flow through the second branch portion 142b. Similarly, in the present invention, the number of the second branch is not limited thereto, and those skilled in the art can design different numbers of the second branch according to actual needs.

In the present embodiment, the mixing tank group 150 includes a first mixing tank 150a and a second mixing tank 150b that communicate with each other. The second branch portion 142b of each of the second flow passages 142 is connected to the mixing groove group 150. As shown in FIG. 10, after the plasma F2 flows to the junction of the second branch portion 142b and the mixing tank group 150, the driving force D6 toward the extending direction of the first mixing tank 150a can be applied to the plasma F2, so that the plasma F2 flows to the first The inside of the mixing tank 150a. Next, as shown in Fig. 11, a driving force D7 toward the extending direction of the second mixing tank 150b is applied to the plasma F2, and the plasma F2 is caused to flow into the second mixing tank 150b.

In the present embodiment, at least one of the first mixing tank 150a and the second mixing tank 150b is disposed with a chemical, or the first mixing tank 150a and the second mixing tank 150b may be disposed with different chemicals. The drug may be disposed in the first mixing tank 150a and the second mixing tank 150b in advance, and after the plasma F2 flows into the first mixing tank 150a and the second mixing tank 150b, the medicine is dissolved in the plasma F2.

In the present embodiment, by alternately applying the driving forces D6, D7, the plasma F2 can reciprocally flow between the first mixing tank 150a and the second mixing tank 150b to be quickly disposed in the first mixing tank 150a and The chemicals in the two mixing tanks 150b are uniformly mixed, thereby reducing the required mixing time.

In addition, as shown in FIG. 11, in the present embodiment, the biometric cartridge 100 further includes an exhaust passage 180. The exhaust passage 180 communicates with the second flow passage 142 to provide the biometric cartridge 100 to vent the gas within the flow-through structure to prevent the detection fluid F from flowing within the flow-through structure due to gas clogging.

Figure 12 is a schematic illustration of a biometric cartridge 200 in accordance with another embodiment of the present invention. FIG. 13 is a partially enlarged schematic view of the biometric cartridge 200 of FIG. Referring to FIG. 12 and FIG. 13, the main difference between the biometric cartridge 200 of the present embodiment and the biometric cartridge 100 of the previous embodiment is that, in the previous embodiment, the biometric cartridge 100 is connected to the branch 122. The narrowing passage 123 and the communication hole 125 correspond to the dosing groove 130. In the present embodiment, the biometric cartridge 100 corresponds to the dosing groove 230 through the partition 290 and the communication hole 225 connecting the branch portion 122.

In the previous embodiment, the biometric cartridge 100 is designed such that the detection fluid F first flows into and fills the dosing tank 130 by the design of the narrowing passage 123 connecting the branch portion 122c and the different cross-sectional width of the communication hole 125. However, a small portion of the detection fluid F may still flow through the reduction passage 123 before the dosing tank 130 is filled. As shown in Fig. 8, in the process in which the plasma F2 flows in the connecting branch portion 122c, most of the plasma F2 tends to pass through the communicating hole 125 having a large flow cross-sectional width W3, and flows to the lower quantitative tank 130. However, a small portion of the plasma F2 may still flow along the connecting branch 122c to the reduced channel 123 having a smaller flow cross-sectional width W2.

Referring to FIG. 12 and FIG. 13 , in the present embodiment, the connecting branch portion 222 c of the first flow path 222 of the biometric cartridge 200 has a partition plate 290 and a communication hole 225 corresponding to each of the dosing grooves 230 . The connecting branch 222c communicates with the corresponding dosing groove 230 through the communication hole 225. The partition 290 is positioned on the communication hole 225, and the communication hole 225 is divided into an inlet 225a and an outlet 225b. The inlet 225a and the outlet 225b of the communication hole 225 are respectively located at two sides of the partition 290.

In the biometric cartridge 200 of the present embodiment, when the plasma F2 flows along the connecting branch 222c to the position corresponding to the dosing tank 230, the plasma F2 is blocked by the partition 290, and flows in from the inlet 225a of the communicating hole 225 first. Slot 230. When the corresponding dosing tank 230 is filled, the plasma F2 will flow out from the outlet 225b of the communication hole 225 and continue to flow along the connecting branch 222c. If the bioassay cassette 200 has a plurality of metering slots 230, the remaining plasma F2 will continue to flow along the connecting branch 122c to a position corresponding to the next metering tank 230, and the above-described flow process is repeated.

In summary, the bioassay cassette of the present invention can effectively reduce the size of the biometric cassette by placing the first layer flow structure and the second layer flow structure on different planes in the biometric cassette. Furthermore, the bioassay cassette of the present invention allows the biodetection cassette of the present invention to exert driving forces in different directions at different timings by designing a plurality of flow channels, separation grooves, dosing grooves and mixing grooves in the extending direction. The detection fluid in the bioassay cassette is sequentially flowed to the corresponding position. Compared with the conventional bioassay cassette which uses capillary action to cause the detection fluid to flow, the flow path size of the bioassay cassette of the present invention does not need to be so fine, so that the convenience of manufacture can be improved. In addition, by connecting the structural design of the corresponding quantitative groove on the branch, the amount of the detection fluid flowing to the mixing tank and reacting with the agent in the mixing tank can be ensured to be within a set range to avoid excessive or too little detection fluid. This causes an error in the detected value. Further, by designing the plurality of mixing grooves, by applying a driving force corresponding to a specific direction, the detection fluid can be quickly mixed with the agent disposed in the mixing tank, thereby reducing the required mixing time.

Although the present invention has been disclosed in the above embodiments, it is not intended to limit the present invention, and any one of ordinary skill in the art can make some changes and refinements without departing from the spirit and scope of the present invention. The scope of the invention is defined by the scope of the appended claims.

A, B‧‧‧ Location on the biometric cassette
D1~D7‧‧‧ driving force
F‧‧‧Detecting fluids, blood
F1‧‧‧ blood cells
F2‧‧‧ plasma
W2, W3‧‧‧ wide flow section
10‧‧‧Detection device
12‧‧‧Rotating base
14‧‧‧First rotation axis
16‧‧‧Second rotation axis
100, 200‧‧‧ biometric cards
110‧‧‧ collection port
120, 220‧‧‧ first-tier circulation structure
122‧‧‧First runner
122a, 122b‧‧‧ first branch
122c‧‧‧First branch, connecting branch
123‧‧‧Shrinking the channel
125, 225‧‧‧Connected holes
130, 230‧‧ ‧Quantitative tank
140‧‧‧Second floor circulation structure
141‧‧ ‧ shunt structure
142‧‧‧Second runner
142a, 142b‧‧‧second branch
150‧‧‧Mixed trough group
150a‧‧‧First mixing tank
150b‧‧‧Second mixing tank
160‧‧‧Separation tank
170‧‧‧Overflow trough
180‧‧‧Exhaust passage
190‧‧‧ venting holes
222c‧‧‧Connected branch
225a‧‧‧Import
225b‧‧‧Export
290‧‧ ‧ partition

1 is a perspective view of a biometric cartridge according to an embodiment of the invention. 2 is a top perspective view of the biometric cassette of FIG. 1. 3A and 3B are schematic diagrams showing an embodiment of a detecting device for a biometric cassette according to an embodiment of the present invention, which respectively shows the relationship between the biometric cassette and the detecting device at different time points. 4 to 11 are schematic views showing the flow of the detection fluid in the bioassay cassette of the embodiment of the present invention. Figure 12 is a schematic illustration of a biometric cartridge in accordance with another embodiment of the present invention. Figure 13 is a partially enlarged schematic view of the biometric cartridge of Figure 12;

100‧‧‧Biometric card

110‧‧‧ collection port

120‧‧‧First floor circulation structure

122‧‧‧First runner

122a, 122b‧‧‧ first branch

122c‧‧‧First branch, connecting branch

123‧‧‧Shrinking the channel

125‧‧‧Connected holes

130‧‧‧Quantitative tank

140‧‧‧Second floor circulation structure

141‧‧ ‧ shunt structure

142‧‧‧Second runner

142a, 142b‧‧‧second branch

150‧‧‧Mixed trough group

150a‧‧‧First mixing tank

150b‧‧‧Second mixing tank

160‧‧‧Separation tank

170‧‧‧Overflow trough

180‧‧‧Exhaust passage

190‧‧‧ venting holes

Claims (16)

A biological detection cassette adapted to collect a detection fluid, the biological detection cassette comprising: a collection port; a first layer flow structure in communication with the collection port; and a second layer flow structure, and the first layer The flow-through structure is in communication, wherein the first layer flow-through structure and the second layer flow-through structure are disposed on different planes in the bio-detection cassette. The biometric cassette of claim 1, wherein the first layer flow structure and the second layer flow structure partially overlap each other. The biometric detection cassette of claim 1, wherein the first layer flow structure comprises: a first flow path, communicating with the collection port and including a plurality of first branches connected by bending; and a separation groove And connecting to a bend between the two of the first branches, and extending the direction of the separation groove parallel to the first branch to which one of the branches is connected. The biometric cassette according to claim 3, wherein the second layer flow structure comprises at least one shunt structure, each of the shunt structures comprising: a quantification slot communicating with the first flow channel; and a second flow channel a plurality of second portions communicating with the quantification tank and including a bend connection; a first mixing tank communicating with the second flow passage; and a second mixing tank communicating with the second flow passage and The first mixing tanks are in communication. The biometric cartridge according to claim 1, wherein the first layer flow structure comprises a first flow channel, and the second layer flow structure comprises at least one flow distribution structure, wherein the first flow channel has a corresponding a reduced passage of the flow dividing structure and a communication hole through which the first flow passage communicates with the flow dividing structure. The biometric cartridge according to claim 5, wherein the communication hole has a flow cross-sectional width that is larger than a flow cross-sectional width of the reduced passage, and the communication hole is located on the detection fluid entry side of the reduced passage. The biometric cartridge according to claim 1, wherein the first layer flow structure comprises a first flow channel, and the second layer flow structure comprises at least one flow distribution structure, wherein the first flow channel has a corresponding a partition of the flow dividing structure and a communication hole through which the first flow path communicates with the flow dividing structure. The biological detection cassette according to claim 7, wherein the partition is located on the communication hole, and the communication hole is divided into an inlet and an outlet, wherein the inlet and the outlet of the communication hole are respectively located Both sides of the partition. The biometric cartridge according to claim 1, wherein the biometric cartridge is adapted to be applied to a detecting device, wherein the detecting device is configured to apply the detecting fluid in the biometric cartridge at different time points. The driving force in the corresponding direction causes the detecting fluid to flow in the first layer flow structure and the second layer flow structure. A method for detecting a fluid flow of a biometric cassette includes: providing a detection fluid to a biometric cartridge; and applying a first driving force corresponding to a first direction to the biometric cartridge at a first time point The detection fluid; and at a second time point, applying a second driving force corresponding to a second direction to the detection fluid in the biometric cartridge, wherein the first direction is different from the second direction. The method for detecting a fluid flow of a bioassay cassette according to claim 10, wherein the bioassay cassette comprises: a collection port; a first layer flow structure communicating with the collection port; and a second layer The flow-through structure is in communication with the first layer flow-through structure, wherein the first layer flow-through structure and the second layer flow-through structure are disposed on different planes within the bio-detection cassette. The method for detecting a fluid flow of a bioassay cassette according to claim 11, wherein the first layer flow structure comprises a connection branch, the second layer flow structure comprises a quantity of grooves, the connection branch and the quantification tank The method of detecting fluid flow, further comprising: applying, at a third time point, a third driving force parallel to an extending direction of the connecting branch to the detecting fluid in the biological detecting cassette, so that the detecting fluid is along The connecting branch flows; wherein, when the detecting fluid flows through the dosing tank, the detecting fluid flows into and fills the dosing tank, and the overflowing detecting fluid flows along the connecting branch. The method of detecting a fluid flow of a biometric cassette according to claim 12, wherein the connecting branch has a reduced passage corresponding to the quantification slot and a communication hole through which the connection branch passes and the quantification slot Interconnected, and the communication section has a flow cross-sectional width that is greater than a flow cross-sectional width of the reduced passage, wherein when the detection fluid flows through the quantification tank, the detection fluid flows from the communication hole and fills the quantification tank, and overflows The detection fluid flows through the reduction passage and flows along the connection branch. The method for detecting a fluid flow of a biometric cassette according to claim 12, wherein the connecting branch has a partition corresponding to the quantification slot and a communication hole through which the connecting branch passes the quantification slot Interconnected, and the baffle is located on the communication hole, the communication hole is divided into an inlet and an outlet, the inlet and the outlet of the communication hole are respectively located at two sides of the partition, wherein when the detection fluid flows Through the dosing tank, the detecting fluid flows in from the inlet of the communicating hole and fills the dosing tank, and the overflowing detecting fluid flows from the outlet of the communicating hole back to the connecting branch and flows along the connecting branch . The method of detecting a fluid flow of a bioassay cassette according to claim 10, wherein the bioassay cassette comprises: a first mixing tank; and a second mixing tank communicating with the first mixing tank, wherein The method for detecting a fluid flow further includes: applying a third driving force toward a direction of extension of the first mixing tank to the detecting fluid in the biometric cartridge at a third time point, causing the detecting fluid to flow into the detecting fluid a first mixing tank; and at a fourth time point, applying a fourth driving force toward the extending direction of the second mixing tank to the detecting fluid in the biometric cartridge, such that the detecting fluid is caused by the first mixing The groove flows into the second mixing tank; wherein the first mixing tank extends in a direction different from the extending direction of the second mixing tank. The method for detecting a fluid flow of the biometric cassette according to claim 15, further comprising: applying a third driving force toward the extending direction of the first mixing tank to the biological detection at a fifth time point The detection fluid in the cassette causes the detection fluid to flow from the second mixing tank into the first mixing tank.