TW201703649A - Method for producing seasoning using fish scales providing fresh taste, nutrition, healthcare and natural features, and capable of being used as calcium supplements - Google Patents

Abstract

The present invention discloses a method for producing seasoning using fish scales, which employs the fish scales after drying and utilizes one of citric acid, oxalic acid, acetic acid, sulfuric acid and hydrochloric acid, or alkaline protease or pepsin enzyme for hydrolysis; next, performing the steps of acid-base neutralization, filtering and heating to obtain the functional fish scale seasoning having fresh taste.

Description

Method for making seasonings with fish scales

The present invention relates to a method of making a food seasoning, and more particularly to a method of making a seasoning with fish scales.

According to the people, the requirements for seasonings are getting higher and higher, not only the color, fragrance and taste are required. It is also expected that the seasonings can have the functions of nutrition, health care, naturalness and diversification, so that the food seasonings are flavored by the past. The main brewing seasoning or chemical seasoning turns to functional seasonings and natural seasonings. However, the current natural seasonings or functional seasonings with additional nutrients have the problems of high raw material cost and complicated process.

Because Taiwan is rich in aquatic resources, it consumes a large amount of aquatic products every year. In the process of aquatic product processing, a large amount of waste-fish scales will be produced. At present, most of them are not properly treated and used, but they are crushed as fertilizer or even discarded, not only wasting resources. Cause environmental pollution. However, fish scales are rich in collagen and hydroxyapatite, and can also be used as an inexpensive raw material for food processing. However, at this stage, the application of fish scales is mainly focused on the extraction technology of collagen in fish scales. For example, the patents of China's inventions 201041522, 200613565 and I362393 are all techniques for extracting collagen from fish scales, resulting in the use of scales. It is still not possible to spread to the general life.

In view of this, how to use fish scales to make natural seasonings is The primary problem to be solved by the present invention is therefore that the inventor of the present invention finally has the invention after continuous arduous thinking and trial work.

The main object of the present invention is to provide a method for preparing seasonings by using fish scales, which has the advantages of low cost and easy manufacture, and is made by collagen and hydroxyapatite which are rich in fish scales. The seasonings can have the characteristics of glycol, nutrients, health care, and natural properties, and can also act as a good calcium supplement to help minerals absorb, thereby reducing the incidence of osteoporosis in consumers.

In order to achieve the above object, the present invention provides a method for making a fish scale seasoning using different acids, comprising the steps of: a. heating the fish scale with water at about 100 ° C, and then drying the fish scale to a water content of 5 to 10%; The dried fish scale is hydrolyzed with one of citric acid, oxalic acid, acetic acid, sulfuric acid or hydrochloric acid at a concentration of 1 to 18%, and the hydrolysis time is 1 to 9 hours, and the temperature is maintained at 100 ° C; c. Potassium oxide or sodium hydroxide is neutralized to the pH of 4.5 to 5 by the hydrolyzed fish squama, and the unhydrolyzed protein is precipitated; d. The solid matter is removed by filtration and heated to a boiling point of at least 10 minutes to obtain the present invention. Fish scales.

The invention further provides a method for preparing a fish scale seasoning by using an enzyme, which comprises the following steps: (1) heating the fish scale with water of about 100 ° C, and then drying the fish scale to a water content of 5 to 10%; (2) boiling the dried fish scales in boiling water for about 10 minutes, and adjusting to the optimum pH and temperature, followed by hydrolysis with a concentration of 1% alkaline protease or pepsin enzyme, the hydrolysis time is 1 to 9 hours; 3) heating the fish scales that have been hydrolyzed to a boiling point for at least 10 minutes to lose the activity of the enzyme, followed by neutralization of the acid and base to a pH of 4.5 to 5 to precipitate the unhydrolyzed protein; (4) filtering to remove the solid matter, ie The fish scale seasoning of the present invention can be obtained.

The above objects and advantages of the present invention will be readily understood from the following detailed description of the embodiments of the invention.

Figure 1 is a flow chart showing the fabrication of the first embodiment of the present invention.

Figure 2 is a flow chart showing the fabrication of the second embodiment of the present invention.

Figure 3 is a flow chart showing the fabrication of the third embodiment of the present invention.

Figure 4 is a graph comparing the solid content of 18% different acid hydrolyzed milkfish fish scale hydrolysate.

Figure 5 is a graph showing the ratio of the ash content of 18% different acid-hydrogenated milkfish ichthyosis hydrolysate in the present invention.

Fig. 6 is a graph showing the comparison of the solid content of the hydrolyzed fish scales of 1% different acid hydrolysis in the present invention.

Figure 7 is a graph showing the ratio of the ash content of the hydrolyzed fish scales of 1% different acid hydrolysis in the present invention.

Fig. 8 is a graph showing the ratio of the solid content of the hydrolyzed fish scales hydrolysate of the different enzymes in the present invention.

Fig. 9 is a graph showing the ratio of the ash content of the hydrolyzed fish scales hydrolyzate of different enzymes in the present invention.

Figure 10 is a graph comparing the calcium ion binding force of 18% different acid hydrolyzed milkfish fish scale hydrolysate.

Fig. 11 is a graph showing the analysis of the calcium ion binding force of the fish carp fish scale hydrolysate of the present invention.

As shown in Fig. 1, a first embodiment of a method for making a seasoning with fish scales according to the present invention mainly comprises the following steps:

a. After heating the fresh milkfish scales with water of about 100 ° C, the microorganisms, bacteria and membranes on the surface of the scales are removed to avoid protein spoilage and astringency, thereby completing the pretreatment treatment, and then the milkfish The fish scale is dried in an oven at 60 ° C to a moisture content of 5 to 10%.

b. hydrolyze the dried milkfish scales with one of citric acid, oxalic acid, acetic acid, sulfuric acid or hydrochloric acid at a concentration of 18%, wherein citric acid, oxalic acid, acetic acid, sulfuric acid or hydrochloric acid and dried milkfish The weight ratio of the fish scale was 2:1, the hydrolysis time was 3, 6, and 9 hours, and the heating was maintained at 100 °C. Hydrolysis of the milkfish scales allows the protein to form peptides, amino acids to increase their nutritional value, and lower the pH to inhibit protein spoilage. However, after the hydrolysis of the general protein hydrolysate, the hydrophobic amino acid is exposed at both ends or branches of the peptide bond, which results in a bitter taste characteristic. In addition, the composition and content of different proteins are different from those of the amino acid. Protein hydrolysate The bitterness intensity will vary greatly. However, the fish scale collagen used in the present invention contains a large amount of glycine acid in its amino acid composition, so the fish scale hydrolyzate is sweeter and bitter than other protein hydrolyzates, and is equivalent. It is suitable as a raw material for seasonings and is also rich in protein and calcium, so it is also an ideal source of calcium supplement.

c. Acid-base neutralization of the hydrolyzed milkfish scales with potassium hydroxide or sodium hydroxide, and adjusted to a pH of 4.5 to 5 to precipitate unhydrolyzed protein. In this example, 1N KOH is used. Perform acid-base neutralization. In the acid-base neutralization step, if potassium hydroxide (KOH) is selected, potassium chloride (KCl) may be precipitated after the reaction, and if sodium hydroxide (NaOH) is selected, sodium chloride may be precipitated after the reaction. (NaCl), whereby the precipitated salts can be controlled to meet actual needs.

d. After filtering and removing the solid matter, and heating to the boiling point for at least 10 minutes, the obtained fish scale protein hydrolyzate can be preserved with its unique taste and rich in amino acid, peptide and moderate salt. It is used directly as a seasoning. As shown in the photo in Annex 1, the finished product can be a seasoning liquid or a seasoning powder type.

Furthermore, it is also possible to add a specific flavor of an auxiliary material (such as a hatfish seasoning, a scallop seasoning, etc.) to the fish scale seasoning prepared by performing all the above steps according to actual needs.

Next, please refer to FIG. 2, which is a second embodiment of the method for preparing a seasoning for fish scales according to the present invention, and the difference from the first embodiment is that the concentration is 1% in step b. One of citric acid, oxalic acid, acetic acid, sulfuric acid or hydrochloric acid, wherein the weight ratio of citric acid, oxalic acid, acetic acid, sulfuric acid or hydrochloric acid to dried milkfish scales is 2:1, and is maintained by autoclaving under pressure The dried milkfish scales were hydrolyzed at 121 ° C and a pressure of 1.21 kg/cm ² for 1 to 3 hours.

As shown in FIG. 3, a third embodiment of the method for preparing a seasoning for fish scales according to the present invention is mainly for using the fish scale seasoning obtained in the first embodiment or the second embodiment of the present invention as a first A raw material is mixed with a second raw material to obtain a mixed fish scale seasoning, and the second raw material is prepared by the following steps:

(1) After heating the fish scale with water of about 100 ° C, the fish scale is dried at 60 ° C to a water content of 5 to 10%.

(2) boil the dried fish scales in boiling water for about 10 minutes, adjust to the optimum pH and temperature, and then add 0.5-3% alkaline protease (Alcalase) or pepsin enzyme to hydrolyze. In the examples, a 1% alkaline protease (Alcalase) or pepsin enzyme was added, and the weight ratio of alkaline protease (Alcalase) or pepsin (Pepsin) to dried milkfish scales was 2:1. The hydrolysis time is 1 to 9 hours. Among them, alkaline protease (Alcalase) is obtained by fermentation of Bacillus licheniformis, and the main component is subtilisin, which is a kind Endonuclease, the catalytic site is serine, the optimal activity conditions are temperature 50 ° C, pH 8.0, so when adjusting the optimum pH value, potassium hydroxide or sodium hydroxide can be added to adjust the pH value. Its protein hydrolysis rate is higher than that of papain, and the bitterness of the product is also low. It has wide specificity for peptide bonds, especially for residues without electrons.

Pepsin is a digestive protease secreted by the main cells of the gastric mucosa in the stomach. Its function is to break down proteins in food into small peptide fragments. Pepsin has high activity in an acidic environment, and its optimum pH is about 3. In a solution of neutral or alkaline pH, pepsin will melt and lose its activity, so when adjusting its optimum pH value, Hydrochloric acid or other acids can be added to adjust the pH. It has good thermal stability and is stable and stable at 60 °C. Its specificity is wide, it will preferentially cleave bonds with its phenyl cyclic amino acid bond, and also hydrolyze amino acids such as glutamic acid and leucine.

(3) heating the fish scales that have been hydrolyzed to a boiling point for at least 10 minutes to lose the activity of the enzyme, and then neutralizing with hydrochloric acid (HCl) or potassium hydroxide (KOH) to a pH of 4.5 to 5 to make the unhydrolyzed Protein precipitation. Among them, alkaline protease (Alcalase) is acid-base neutralized with hydrochloric acid (HCl), and if it is pepsin, it is neutralized with potassium hydroxide (KOH).

d. The second raw material can be obtained by filtering to remove its solid matter.

And mixing the first raw material and the second raw material with each other The fish scales seasonings are also unique and delicious, and are rich in amino acids, peptides and moderate salts.

Next, the following experimental examples are combined with the test results to further illustrate the achievable effects of the present invention.

The analysis method is as follows:

1. Color Analysis - Place the sample into the sample and measure it with a color difference meter (BYK-Gardner). L value = 100 means that all white is the brightest, L value = 0 means all black; a value: positive red, negative Green; b value: positive yellow, negative blue.

2. Total nitrogen determination - take 1 mL of sample and add 30 mL of concentrated sulfuric acid and catalyst (5g K ₂ SO ₄ ) to the liquid to be transparent and colorless at 400 °C. After the sample is cooled, add 50 mL of distilled water, 32% NaOH, 120 mL, and distill for 6 minutes. 40 mL of 4% boric acid and two drops of indicator (BCG plus MR) were titrated to a discoloration using a 0.2 N sulfuric acid standard solution. The total nitrogen content of the sample is calculated as follows: Total nitrogen content (%) of the sample = (AB) × N × 14.01 / (sample (mL) x 10)

A: The amount of sulfuric acid used for titration

B: the amount of sulfuric acid used in the blank group titration

N: equivalent concentration of sulfuric acid used for titration

3. Determination of formaldehyde nitrogen - Take 20mL sample in a 50mL quantitative bottle, add water to volume, take 25mL in a beaker, adjust to 0.15N with 0.1N sodium hydroxide and then add 20mL of formaldehyde solution to 0.1N Nitrogen The sodium was titrated to pH 8.5. The formaldehyde content of the sample was calculated as follows: Sample formaldehyde nitrogen content (%) = 0.0014 × V × F × (50 / W) × (100 / 20)

V: 0.1N sodium hydroxide titration

W: sample capacity (mL)

F: concentration coefficient of 0.1N sodium hydroxide

4. Degree of hydrolysis - Degree of hydrolysis (%) = Formaldehyde nitrogen / Total nitrogen × 100

5. In vitro digestion test - The sample was adjusted to pH 0.2 with 0.2 N HCl, and added with a base mass of 1% Pepsin for 4 hours in a 37 ° C water bath, then adjusted to pH 7.0 with 2N NaOH, followed by addition of 1% Pancreatin. The reaction was carried out in a 37 ° C water bath for 4 hours, and the reaction was terminated by boiling water for 10 minutes to inactivate the enzyme. Finally, the following experiment was carried out by filtration.

6. Determination of calcium binding capacity - 20 mL of sample was added to 20 mL of 15 mM CaCl ₂ , then 20 mL of 0.06 M potassium phosphate buffer solution was added, and the pH of the reaction solution was maintained at 7.5 hours with alkali stirring. After the reaction was completed, centrifuged at 3500 rpm for 20 minutes, and the supernatant was taken. 0.1 mL of the solution was uniformly mixed with 2 mL of o-Cresolphthalein Complexone coloring agent, then 2 mL of AMP buffer solution was added, and the absorbance was measured at a wavelength of 570 nm in the dark for 15 minutes, and the sample was subjected to a standard curve to convert the sample calcium ion concentration (ppm). ).

7. Sensory evaluation analysis - with a preference score (hedonic-scale Test) The sample is subjected to a taste test. The seven-point system is adopted. The 7-point representative is very fond of it. The 4-point representative does not like it and is not annoying. One-point representative is very disliked. The sensory evaluation of the product's color, aroma, flavor, salinity and general rating was carried out by 12 semi-trained personnel through a hobbyist evaluation. The score table is shown in Table 9.

8. Statistical analysis - The five-fold data obtained from each experiment was analyzed by SPSS (Statistical Package for Social Science) statistical software. The results were analyzed by one-way variance (mean ± SD) using one-way variance (one-way) Analysis of variance, one-way ANOVA) and paired sample assay (T-test) for variant analysis, and Ducan's multiple analysis of significant differences between the mean values.

The following is a general component analysis of the milkfish scales, 18% analysis of different acid hydrolyzed milkfish scales, 1% analysis of different organic acid hydrolyzed milkfish scales, and analysis of enzyme hydrolyzed milkfish scales according to the above analysis method. The preparation, sensory evaluation analysis and correlation of the milkfish fish scale protein hydrolyzed seasoning made by the present invention were compared and analyzed.

Analysis of general composition of fish scales of milkfish

The main components of fish scales are protein and ash, while the protein and ash content of cultured and wild milkfish scales are 61%, 56%, 36%, and 38%, respectively. Table 1 shows the general composition of the dried milkfish scales of the present invention, and the results show that The dried milkfish scales contain a higher protein content of 56.84% followed by 41.03% of the ash, while the fat content is at least 0.36%. The milkfish scales have low fat content and high protein content, so the dried milkfish scales are good raw materials for protein hydrolysate.

Analysis of 18% different acid hydrolyzed milkfish scales

(1) Solids and ash content

Figure 4 shows the solid content of 18% different acid extracted milkfish scales hydrolysate. The results showed that the oxalic acid treatment group had a higher solid content. In terms of ash, the results in Figure 5 show that the highest ash content in the hydrochloric acid treatment group at each hydrolysis time is 9.81%, 9.67%, and 9.42%, respectively, and the high ash content is adjusted at pH. The value is caused by the precipitation of salts. When an acid reacts with a highly active metal such as calcium, magnesium, aluminum, zinc, iron, etc., it will produce salts and hydrogen, and if sodium hydroxide is used in adjusting the pH, the salt precipitated from the hydrolyzate should be It is a potassium salt.

(2) Color analysis

In terms of color analysis, Table 2 shows that the L value will decrease with the increase of hydrolysis time, and the measured value of citric acid treatment for 3 hours is higher, and the measured value is 64.03. On the contrary, the a value and the b value increase with the increase of the hydrolysis time, and the high value of a and b are 9 hours after the sulfuric acid treatment, and the measured values are 9.58 and 39.82, respectively.

(3) Total nitrogen, formaldehyde nitrogen and degree of hydrolysis

The degree of protein hydrolysis is an indicator for determining that the protein is hydrolyzed into a low molecular weight peptide substance and an amino acid. Table 3 shows the total nitrogen, formaldehyde nitrogen and degree of hydrolysis of the milkfish scales hydrolyzate extracted by 18% different acids. The results showed that the total nitrogen, formaldehyde nitrogen and hydrolysis degree increased with the increase of hydrolysis time. From the results of total nitrogen, the total nitrogen content of the citric acid and oxalic acid treatment groups was higher than that of the other treatment groups. The formaldehyde nitrogen and the degree of hydrolysis were all higher in the hydrochloric acid treatment group, and the highest degree of hydrolysis was observed in hydrochloric acid for 9 hours, and the measured value was 49.71%.

(4) Calcium ion binding force

Calcium digestion is mainly in the human intestinal system, so this experiment simulates the human digestive system to analyze the calcium ion binding force of the milkfish tartaric acid hydrolysate. Figure 10 shows that all treatment groups have the calcium ion binding force before undigestion. Higher, all treatment groups treated with sulfuric acid (D) and hydrochloric acid (E) for 9 hours had higher binding strength, and the measured values were 15.33 ppm and 15.60 ppm, respectively.

Analysis of 1% Different Organic Acid Hydrolyzed Milkfish Scales

(1) Solids and ash content

Fig. 6 and Fig. 7 show the solid content and ash content of 1% different organic acid hydrolyzed milkfish scales. The results show that the content of ash and solids increases with time, and the solid content of sulfuric acid treated group The ash content was higher than that of the other treatment groups, and the higher measured values were treated with sulfuric acid for 3 hours, which were 3.75% and 0.76%, respectively.

(2) Color analysis

In color analysis, a positive value of L value represents brightness and a negative value represents darker color, a positive value of a value represents red and a negative value represents green, and a positive value of b value represents yellow, whereas a negative value represents blue. Table 4 shows the color analysis of 1% different acid-extracted milkfish fish scale hydrolysate. The results showed that the L value was 2 hours after sulfuric acid treatment, and the measured value was 66.48. The a value was higher in the acetic acid treatment group than in the other groups, and the higher the measured value was 1.32 after treatment with acetic acid for 2 hours.

(3) Total nitrogen, formaldehyde nitrogen and degree of hydrolysis

Generally, the higher the degree of hydrolysis over time, the case where the protein is hydrolyzed to become a peptide of a small molecule or a free amino acid, which can be expressed by a degree of hydrolysis (DH). Table 5 shows the total nitrogen, formaldehyde nitrogen and hydrolysis degree of 1% different acid extraction of milkfish scales hydrolysate. The results show that the results of each experiment increase with the extraction time. In terms of degree of hydrolysis, the hydrochloric acid treatment group was higher than the other experimental groups, and the extraction with hydrochloric acid for 2 hours had a higher value, and the measured value was 43.09%.

Analysis of Enzymatic Hydrolyzed Milkfish Scales

(1) Solids and ash content

Figure 8 shows the solid content of the hydrolysate of the milkfish scale extracted by the enzyme, and the ash content of the hydrolyzate is shown in the figure 9. The results show that the ash and solid content will increase with time, among which The content of ash and extracts in the pepsin treatment group was higher than that in the alkaline protease treatment group, and the measured values were 1.52% and 0.23%, respectively.

(2) Color analysis

Table 6 shows the color analysis of the hydrolyzate of the milkfish scales extracted by enzyme. The L value of each treatment group increased with the increase of hydrolysis time. Among them, the pepsin treatment group was higher than the alkaline protease, and the highest L value was pepsin. Hydrolyzed for 10 hours, the measured value was 64.79. On the contrary, the b value was higher in the alkaline protease-treated group, and the higher the measured value was 5.11 after hydrolysis with alkaline protease for 8 hours.

(3) Analysis of total nitrogen, formaldehyde nitrogen and hydrolysis degree

Table 7 shows the total nitrogen, formaldehyde nitrogen and hydrolysis degree of the hydrolysate of the milkfish scales extracted by the enzyme. The measured value of the formaldehyde nitrogen in the alkaline protease treatment group increased with time, whereas the pepsin treatment group No significant difference. In terms of degree of hydrolysis, the hydrolysis degree also increased the degree of hydrolysis, and the highest degree of hydrolysis was obtained by treating with alkaline protease for 10 hours. Many studies on related protein hydrolysis have also shown that Alcalase is a hydrolyzing enzyme with high capacity after hydrolysis and better performance in subsequent functional studies.

(4) analysis of calcium ion binding force

Fig. 11 is a graph showing the binding force of calcium ion in the hydrolysate of the milkfish scale. The alkaline protease treatment group increased the calcium ion binding capacity after digestion test, and the higher the measured value was treated with the digested alkaline protease for 12 hours. The measured value was 13.56 ppm. On the other hand, the pepsin treatment group may pass the digestive tract test because the molecule is too small to bind to calcium ions, so the value of the undigested treatment is higher than that of the group treated by the digestive tract test. Since the protein hydrolysate is ingested through the mouth, the gastrointestinal digestive system in the body digests the peptide or protein, and the physiologically active peptide is again hydrolyzed to reduce the activity or produce more active peptides.

Preparation, Sensory Evaluation and Correlation Analysis of Protein Hydrolyzed Seasoning of Milkfish Fish Scale

(1) Analysis of color, salinity and viscosity

Table 8 shows the color, salinity and viscosity analysis of the protein hydrolyzed seasoning of the milkfish scales. The product made by the enzyme treatment group in the L value has the largest L value, and the measured value is 62.82. Commercially available products have a minimum L value of 10.92, which means that the color of the commercially available product is darker than the color of the product we make. In addition, in the case of a value, the maximum value is 28.18, which means that the commercially available product is more reddish in addition to the darker color, and it also affects the dyeing of the ingredients during cooking. Also commercially available in terms of salinity The product has the highest salinity of 42.00%, which is close to the value of 41.67% of the product made with 18% acid. In terms of viscosity, the product prepared by 18% acid treatment has the highest viscosity, the measured value is 16.30 cp, followed by the commercially available product (11.80 cp), and the viscosity of the product prepared by enzyme treatment and 1% acid treatment is biased. Low, viscosity measurements were 2.63 cp and 2.39 cp, respectively.

(2) Sensory evaluation analysis

Table 9 shows the model of the sensory evaluation score sheet.

Table 10 is the sensory evaluation of the protein hydrolyzed seasoning of the milkfish scales. The results show that in terms of color, the commercially available products are less favored by the tasters because of the darker colors, and the 18% treatment group in the aroma is less acceptable. In addition, in terms of salinity, the 18% treatment group had the strongest intensity, but this also resulted in a lower score of 3.88 in the saltiness preference, while the weakest enzyme treatment group had a higher The scores of preferences and total scores were 5.13 and 5.50 respectively.

(3) Correlation analysis

Table 11 shows the correlation analysis of the color, salinity and sensory evaluation of the protein hydrolyzed seasoning of the milkfish scale. It can be seen from the table that the L value is negatively correlated with the a value (p<0.01), while in the senses. In terms of evaluation, flavor and aroma were positively correlated (p<0.01), while the main items affecting the overall evaluation were color, aroma, flavor and preference for salinity, and they were all positively correlated with total (p<0.01). It also means that as their scores rise, the overall rating will also rise.

Through the above comparison analysis, it was found that in the case of extracting the milkfish scales with 18% organic acid, the oxalic acid treatment group had a higher solid content, and the ash content was the highest in the hydrochloric acid treatment group. In terms of total nitrogen, formaldehyde nitrogen and degree of hydrolysis, the prolongation of time leads to an increase in the value, while the formaldehyde nitrogen and hydrolysis degree of the hydrochloric acid treatment group have higher measured values.

In addition, with the increase of time, the content of ash and solids of the fish scales hydrolyzed by 1% different organic acids will increase, and the higher measured value is treated with sulfuric acid for 3 hours. In terms of degree of hydrolysis, extraction with hydrochloric acid for 2 hours has a higher value.

Furthermore, the use of enzymes to extract the milkfish scales hydrolysate increases the amount of ash and solids over time, with the highest value in the pepsin treatment group. The increase in the degree of hydrolysis is mainly due to the prolongation of the hydrolysis time, while the alkaline protease produces the best degree of hydrolysis.

Finally, after seasoning the milkfish fish scale protein hydrolysate and making it into a seasoning liquid, it was found that the marketed seasoning liquid had the highest salinity, a value and low L value, which also showed that the marketed seasoning liquid was too salty. In the case of the situation, it is easier to dye the food in the application, and the color that is too deep in the sensory evaluation is not favored by everyone. The intensity and preference of the saltiness are also a major condition affecting the overall evaluation, so in the seasoning liquid The enzyme treatment group with the weakest saltiness has the highest overall score, while the 18% of the most salty treatment group is the least popular.

And because the collagen of the milkfish scales contains high amounts of glycine and a lot of glutamic acid and alanine, these amino acids have a good taste, and the protein of the scales contains glycine after hydrolysis. The acid oligopeptide is delicious and does not have a bitter taste like other hydrolyzed proteins that are rich in hydrophobic amino acids. In addition, the oxyapatite in the scales can also act as a good calcium supplement. Many studies have shown that glycine-containing peptides can help minerals, so the fish scales made from fish scales are more Can effectively reduce the incidence of osteoporosis in consumers. And its antioxidant capacity, calcium ion binding ability, DPPH free radical scavenging ability, etc. have a good performance, and can constitute a delicious and functional fish scales, which can not only effectively use domestic aquatic processing waste, It can also give Chinese people new and healthy new choices, and has deep development potential and industrial utilization.

However, the disclosure of the above embodiments is for explaining the present invention and is not intended to be used. The present invention is not intended to be limited by the scope of the present invention.

In summary, it will be apparent to those skilled in the art that the present invention can achieve the foregoing objectives and is in compliance with the provisions of the Patent Law.

Claims (7)

A method for making seasonings with fish scales, comprising the steps of: a. heating the fish scales with water at about 100 ° C, and then drying the fish scales to a water content of 5 to 10%; b. citrate and oxalic acid at a concentration of 1 to 18% , one of acetic acid, sulfuric acid or hydrochloric acid, the dried fish scale is hydrolyzed, wherein the weight ratio of citric acid, oxalic acid, acetic acid, sulfuric acid or hydrochloric acid to fish scale is 2:1, hydrolysis time is 1 to 9 hours, and heated Maintained at 100 ° C; c. Neutralize the hydrolyzed squaraine with potassium hydroxide or sodium hydroxide to a pH of 4.5 to 5 to precipitate the unhydrolyzed protein; d. Filter to remove the solids and heat to the boiling point At least 10 minutes, you can get a fish scale seasoning. The seasoning method according to claim 1, wherein in the step b, one of citric acid, oxalic acid, acetic acid, sulfuric acid or hydrochloric acid having a concentration of 1% is added, and the autoclave is heated under pressure. The dried fish scales were hydrolyzed at 121 ° C and a pressure of 1.21 kg/cm ² for 1 to 3 hours. The seasoning method according to claim 1, wherein after heating to the boiling point for 10 minutes in the step d, an auxiliary material having a specific flavor is further added to the fish scale seasoning. The method for preparing a seasoning according to claim 1, wherein the obtained fish scale seasoning is used as a first raw material and mixed with a second raw material to obtain a mixed fish scale seasoning, and the first The two raw materials manufacturing system includes the following steps (1) After heating the fish scale with water of about 100 ° C, the fish scale is dried to a water content of 5 to 10%; (2) the dried fish scale is boiled in boiling water for about 10 minutes, and adjusted to an optimum pH and temperature, and then Hydrolysis is carried out by adding 0.5% to 3% of alkaline protease or pepsin enzyme, wherein the ratio of alkaline protease or pepsin to fish scale is 2:1, hydrolysis time is 1 to 9 hours; (3) hydrolysis is completed. The fish scale is heated to the boiling point for at least 10 minutes to lose the activity of the enzyme, and then neutralized to pH 4.5 to 5 with hydrochloric acid or potassium hydroxide acid base to precipitate the unhydrolyzed protein; (4) the solid matter is removed by filtration, ie This second raw material can be obtained. The seasoning method for fish scales according to claim 4, wherein in step b, a 1% alkaline protease enzyme is added, and the optimum pH is 8.0, and the optimum temperature is 50 °C. The seasoning method for fish scales according to claim 4, wherein in step b, a pepsin enzyme having a concentration of 1% is added, and the optimum pH is 3.0, and the optimum temperature is 50 °C. The seasoning method according to claim 4, wherein after the solid matter is removed by filtration in the step (4), an auxiliary material having a specific flavor is further added to the second raw material.