TW201643296A - Method and device for marking fibrous materials - Google Patents

Abstract

A method of marking fibers, wherein the method includes providing a plurality of fibers; depositing a marker onto at least a portion of the fibers, the depositing being performed with a delivery mechanism comprising one or more outlets; and thereby marking the fibers. Also provided is a device for marking fibers, including a transport system adapted to transport fibers in a direction of a marker delivery apparatus positioned along the transport system; the delivery apparatus includes one or more outlets, adapted to deposit a solution of the marker through the outlets onto at least a portion of the fibers; and thereby marking the fibers. Authentication of a fibrous material using the marking method of the invention followed obtaining a sample of the marked fibers and assaying the sample for the presence of the nucleic acid marker; and thereby determining whether the fibrous material is authentic or counterfeit.

Description

Method and device for marking fiber material

The present invention relates to a method and apparatus for marking fibrous materials such as cotton, and more particularly to a method and apparatus for marking cotton and cotton products such as yarns, textiles and cotton-containing articles, identified and verified by a nucleic acid marker The source and authenticity of the item.

Cotton is an important economic crop around the world, and many parts of cotton crops are available; however, cotton is especially important for making a variety of goods, such as cloth, clothing, and a variety of household items such as towels and tablecloths. Making a fabric from cotton begins with the processing of a bundle of cotton to release the cotton, which is typically disassembled by an automated machine to remove untreated lint, which can be further cleaned, for example, by using a blower. To separate the staple portion of the lint in the cotton fiber, which can then be woven into a long chain sometimes referred to as a cotton yarn. The woven cotton fiber is used to make many different items, such as cloth, clothing, and household items.

1 lb. cotton can provide tens of thousands of cotton fibers. However, the length of a single cotton fiber varies depending on the species or variety of the cotton crop. The quality of the fabric made from cotton is different depending on the length of the single cotton. . The shorter cotton fibers are usually from cotton varieties such as G. hirsutum , G. herbaceum and G. arboreum . It is generally considered that the shorter cotton fiber quality is worse than the long cotton fiber from the G. barbadense cotton variety, G. barbadense. Derived cotton fiber is commonly referred to as Extra Long Staple (ELS) cotton. ELS cotton is generally considered to be used to make higher quality and high value fabrics, clothing, household goods and related products. ELS cotton types include, for example. American Pima, Egypt, Indian Suvin, products with ELS labels, such as the above-mentioned labels for American Pima, Egypt, and Indian Suwen, compared to the lack of such labeling The product will receive a higher price.

The difference in cotton quality leads to the authenticity and accurate identification of high quality cotton products. The concern is that once raw cotton or cotton-containing products enter commercial circulation involving global trade, it is often difficult to determine with certainty whether the claimed ELS cotton is actually true, or is blended, or completely stapled. It is also difficult to judge whether a particular cotton product originates from a specific place, region, or manufacturer. For example, a counterfeit product made of staple fiber cotton may be improperly or fraudulently labeled as ELS cotton. In the United States, Pima cotton, Egyptian cotton and Indian cotton, cotton products may also be fraudulently marked as coming from a specific region of the world (such as Egyptian cotton). Therefore, an urgent problem to be solved is to provide a method of judging whether a particular item containing cotton is composed entirely of genuine ELS cotton, or whether the counterfeit item contains a significant amount or consists entirely of short staple cotton.

Counterfeiting with low-cost materials and blending into high-end products has become a major problem for big brands. The International Chamber of Commerce (ICC) pointed out in 2008 that counterfeit goods caused a loss of $650 billion in revenue and a reduction in 2.5 million jobs. The International Chamber of Commerce estimates that the loss of revenue will exceed $1.7 trillion in 2015. This is equivalent to 2% of the global economy. In addition to loss of income, some counterfeit products directly involve serious health and safety issues, and counterfeit goods have penetrated most industries, from textiles to microchips and even the pharmaceutical industry.

Exemplary embodiments of the present invention provide a method of marking a fibrous material, such as cotton or an article comprising a fibrous material, the method comprising depositing a solution comprising a nucleic acid marker onto at least a portion of the fibrous material, The deposition system is performed using a delivery mechanism comprising more than one nozzle, and the nucleic acid marker can be activated, for example, by adding a functional group to the nucleic acid marker.

According to an exemplary embodiment of the present invention, the nucleic acid marker may comprise DNA, and in another exemplary embodiment, the DNA may be activated by a base, and in another exemplary embodiment, the nucleic acid may be regulated by, for example, a metering controller. The amount of solution of the marker deposited on the fibrous material or article made from the labeled fibrous material.

In another exemplary embodiment, the marked fibrous material may comprise a material such as textile, fiber, cotton, ginned, blended cotton, wool, yarn, nylon or Kashmir wool, the marked fibrous material may comprise synthetic fibers or Synthetic blended fiber, for example comprising ruthenium, nylon, wool or polyester, the polyester synthetic fiber may comprise a monomer, a copolymer, an aliphatic and/or an aromatic, The polyester synthetic fibers may comprise any suitable polyester, such as polyethylene, polypropylene or polyethylene terephthalate, and may be blended with other fibers such as cotton.

In an embodiment of the invention, a method of marking a fiber is provided, wherein the method comprises: providing a plurality of fibers during a manufacturing process; depositing a marking solution on at least a portion of the fibers during the manufacturing process, The deposition is performed using a transport mechanism comprising more than one nozzle; and thus the fibers are marked.

In another embodiment of the present invention, an apparatus for marking a fibrous material is provided, the apparatus comprising: a transport system adapted to transport fibers in a direction of a marking transport mechanism, the marking transport mechanism being disposed along the transport system, Wherein the marker transport mechanism comprises more than one nozzle adapted to deposit a solution comprising the marker on at least a portion of the fibers through the one or more nozzles; and thereby marking the fibers.

In another embodiment of the present invention, a method of verifying a fibrous material is provided, comprising: providing a plurality of fibers; depositing a nucleic acid marker on at least a portion of the fibers, the deposition utilizing delivery comprising more than one nozzle The mechanism performs; producing a labeled fiber; taking a sample of the labeled fibers and analyzing the presence of the nucleic acid marker in the sample of the labeled fibers; and thereby determining whether the fibrous material is true or counterfeit.

110‧‧‧ stage

120‧‧‧nucleic acid marker

130‧‧‧Alkaline activator

140‧‧‧ stage

150‧‧‧ stage

210‧‧‧Refinement trough

211‧‧‧Transportation belt

220‧‧‧ storage bucket

230‧‧‧ indicator

240‧‧‧ pump

250‧‧‧Metric controller

260‧‧‧Transportation agency

270‧‧‧ nozzle

310‧‧‧Locked space

410‧‧‧Selection/Washing Machine

420‧‧‧Transportation belt

430‧‧‧Boom

440‧‧‧

510‧‧‧Manual sprinkler

520‧‧‧ Spray handle

530‧‧‧Transport hose

540‧‧‧Quick disconnect component

Figure 1 shows a schematic view of a cotton processing system in which the raw cotton bales are taken apart and sent to the system through a feeder, through more than one tandem dryer and cleaners and a stick machine to remove the cotton. With the chips, the cotton is then processed through a cotton gin and lint cleaner prior to pressing into a 500 lb. standard sized package; Figure 2 illustrates the deposition of nucleic acid markers on the article or fiber material in accordance with an exemplary embodiment of the present invention. And a device configured to perform a method of depositing a nucleic acid marker on an article or fibrous material; and FIG. 3 illustrates a method of depositing a nucleic acid marker on an article or fibrous material in accordance with an exemplary embodiment of the present invention, and configured to implement Apparatus for the method of depositing a nucleic acid label on an article or fibrous material; Figure 4 is a view showing the verification data of capillary electrophoresis tracking of the polymerase chain reaction (PCR) product of the labeled raw wool; Figure 5 shows the validation data for capillary electrophoresis traces of the PCR products of the labeled unprocessed wool; Figure 6 shows the validation data for capillary electrophoresis traces of the PCR products from the labeled unprocessed wool; Figure 7 shows the unprocessed and Validation data for capillary electrophoresis traces of PCR products of processed textiles; Figure 8 shows verification data for capillary electrophoresis traces of PCR products from textiles before and after multiple dry cleaning cycles; Figure 9 shows Validation data for capillary electrophoresis tracking of PCR products of upholstery textiles made from fibers of nucleic acid markers; Figure 10 shows verification data for capillary electrophoresis of PCR products of carpets made from fibers labeled with nucleic acid markers; and 11th The figure shows a schematic representation of a Lint Flue system for marking cotton fibers with DNA in accordance with an embodiment of the present invention.

Exemplary embodiments of the present invention will be described in more detail hereinafter with reference to the accompanying drawings.

Exemplary embodiments of the present invention provide a method of marking a fibrous material, the method comprising depositing a solution comprising a nucleic acid marker on at least a portion of the fibers, the depositing being performed using a transport mechanism comprising more than one nozzle, The nucleic acid marker can be activated, for example, by an alkaline pretreatment method as described in U.S. Patent No. 2014/0256881, or by the addition of a reactive functional group to the nucleic acid marker.

Marker molecule

In an exemplary embodiment of the invention, a marker molecule deposited, attached, attached or bonded to the fiber or article to be labeled may be a biomolecule (eg, a nucleic acid marker), and the marker molecule may be an inorganic molecule and More than one metal, non-metal or rare earth metal may be included. The biomolecule may be a protein, a peptide, a nucleic acid, a vitamin, or a protein-DNA complex, and the nucleic acid may comprise, for example, an RNA, a DNA, an RNA-DNA complex, a single helix DNA or a double helix DNA, and the nucleic acid may be any suitable one. Size, for example, the nucleic acid can range from about 50 to 1000 base pairs The nucleic acid may comprise any suitable natural or non-native DNA sequence, such as a synthetic DNA sequence, ie, a DNA sequence that is not native, which may be formed by digestion, degradation and re-ligation of natural or non-naturally occurring DNA. . The DNA may be of any source, such as animal or plant DNA, which may be obtained from a bacterial, viral, fungal or synthetic vector or fragment, or any combination thereof, which may comprise degradation and re-attachment of an animal or plant by, for example, digestion. The non-naturally occurring DNA formed by the DNA may comprise synthetic DNA or semi-synthetic DNA composed of a combination of synthetic DNA and semi-synthetic DNA, and the nucleic acid may comprise nuclear, mitochondrial or chloroplast DNA or total genomic DNA.

In an exemplary embodiment of the invention, the nucleic acid marker can be obtained from any suitable source of DNA, such as DNA extracted from a plant source. Nucleic acid markers comprising DNA are interchangeably referred to as DNA tags, and the extracted DNA can be specifically or randomly digested for degradation and ligation to produce world-specific artificial nucleic acid sequences, which are compliant with standard standards well known in the art of molecular biotechnology. Digestive degradation and ligation technology, complete digestion and degradation of extracted DNA and ligation. Digestive degradation can occur randomly or site-specifically by, for example, random or site-specific nucleases, and the nucleic acid fragments resulting from digestion degradation can be specifically or randomly recombined to form new nucleic acid sequences (eg, non-natural nucleic acids) Sequence), the sequence of the nucleic acid marker can be of any suitable length, such as from about 5 to 5000 bases, or from about 20 to 1000 bases.

In an exemplary embodiment of the invention, the nucleic acid marker can comprise activated DNA, or any suitable functionalized DNA, such as alkaline pH activated DNA (see below). The method can include depositing a nucleic acid marker on the surface of the fiber or article to be labeled, or dissolving in a solution to attach, attach or bond the activated nucleic acid marker to the fiber or article, such as in a fiber or article. On the surface or on a part of its surface. The nucleic acid marker can be incorporated into the material or form part of the material of the article. An alkaline pH activated nucleic acid marker comprising an alkaline activating DNA can be bonded to the material, such as cotton, wool, nylon, plastic, metal, glass, wood, or printing ink. The alkaline activation of nucleic acid markers will be discussed in more detail below.

The term nucleic acid is abbreviated as "NA" in the figure and may mean deoxyribonucleic acid (DNA) or ribonucleic acid (RNA), which may comprise an animal, plant, bacterium, virus, fungus, or synthetic vector or fragment or Any combination of nucleic acids. The nucleic acid marker can be any suitable nucleic acid, such as synthetic non-native DNA, semi-synthetic DNA from natural and synthetic sequences, or recombinant native DNA sequences, which are obtained by cleavage and ligation of fragments in new non-native sequences.

The nucleic acid marker can have a specific template sequence and/or a specific template length, such that when the polymerase chain reaction (PCR) step is performed, the PCR primer can be any specific primer pair having a complementary nucleic acid sequence that can be ligated to the nucleic acid Logo template for nucleic acids. There may be a lower nucleic acid concentration in the nucleic acid marker, and the nucleic acid can be signal amplified by techniques well known in the art of molecular biology.

The nucleic acid marker can be mixed with water or any desired aqueous or buffer solution to form a solution containing the nucleic acid marker for use in the methods of the invention, for example, the nucleic acid can be mixed with water to form a solution comprising the nucleic acid marker. The solution containing the nucleic acid marker can be mixed to any desired concentration to label the fiber or article to be labeled. For example, the concentration of the nucleic acid in the solvent can be about 1 gram/ml (10 ^-18 g/ml), 1 gram per milliliter. (10 ^-15 g/ml), 1 pg/ml (10 ^-12 g/ml), 1 nm/ml (10 ^-9 g/ml) or 1 μg/ml (10 ^-6 g/ml), Alternatively, the concentration of the nucleic acid in the solution may range from 1 gram per milliliter (10 ^-18 g/ml) to 1 microgram/ml (10 ^-6 g/ml). A solution of a nucleic acid marker can comprise more than one nucleic acid marker.

Those skilled in the art will also appreciate that a nucleic acid marker can be combined with more than one optical indicator, such as an infrared marker, for example, the optical indicator can be chemically linked to a nucleic acid marker, or the optical indicator can be mixed to include a nucleic acid marker In the solution, the optical indicator can be, for example, an upconverting phosphorescent or fluorophore. The nucleic acid marker and the optical indicator can be mixed during the dyeing process, and the combination or mixing of the nucleic acid marker and the optical indicator can be applied to more than one article to be marked, such as a fiber or fiber material. The fibers or fibrous materials can be materials suitable for incorporation into a textile, and the labeled fibers are then blended with more than one unlabeled fiber to form a labeled textile, the labeled fibers and unlabeled fibers can be Blending during the ginning process, before the opening of the cotton, during the opening process, before the blending, or during the blending process. The fibers may be unprocessed fibers and may be marked during or after scouring, and unprocessed fibers (eg, raw cotton fibers or unprocessed wool fibers) may be referred to as ginned, or ginned and scoured fibers. For example, unprocessed fibers separated from cotton crop material by ginning but not yet scoured may contain small plant parts and foreign matter that were not removed during the ginning process.

Nucleic acid activation

Nucleic acids (e.g., DNA) can be activated to increase the binding strength between the nucleic acid and the fiber or article to be labeled by methods well known in the art (e.g., see GTHermanson, Bioconjugate Techniques , 2d ed., 2008, Academic Press). The activated nucleic acid can cause a physical or chemical reaction of the nucleic acid with the surface of the fiber or article to be labeled (eg, providing a nucleic acid capable of ionic or covalent bonding to an effective functional group on the surface of the article), for example, Nucleic acids can be activated by exposure to alkaline conditions. Alkali activation of nucleic acids can be achieved by the method described in U.S. Patent No. 2014/0256881.

The reactive functional group can be bonded to the nucleic acid to increase the binding strength between the nucleic acid and the fiber or article to be labeled, which can be bonded to the DNA molecule by an alkali activation step (described in more detail later). A nucleic acid, the reactive functional group capable of covalently binding to an effective functional group on at least a portion of the fiber or article to be labeled, the reactive functional group immobilizing the nucleic acid on the fiber or article.

The nucleic acid may be bonded to at least a portion of the surface of the fiber or article to be labeled by chemical linkage bonding of the reactive functional group. For example, the chemical linkage may comprise a carbon chain having a reactive functional group at the end of the carbon chain, on the carbon chain. The other end of the reactive functional group can be covalently bonded to the nucleic acid, and the reactive functional group can be activated to covalently bond with an effective functional group on the fiber or article, and activation of the reactive functional group can be It is carried out by exposure to alkaline conditions.

Forming a solution containing a nucleic acid marker for textile applications

The solution containing the nucleic acid marker can be formed by mixing the nucleic acid in water, and the concentrate of the nucleic acid marker can be mixed with water to form a solution containing the nucleic acid marker before the solution containing the nucleic acid is deposited on the fiber or article. The nucleic acid marker can be activated by a base, for example, the nucleic acid marker can be exposed to the alkaline conditions detailed above, providing an alkaline activator such that it can be mixed with a solution comprising the nucleic acid marker to form an activated nucleic acid marker. The solution comprising the nucleic acid marker can be an aqueous solution comprising a nucleic acid marker, and the solution comprising the nucleic acid marker can be any suitable working solution, such as an aqueous solution which can comprise a buffer solution.

In an exemplary embodiment of the present invention, the aqueous solution containing the nucleic acid marker comprises a non-aqueous solution (for example, polyurethane or eucalyptus oil), and the solution containing the nucleic acid marker can be mixed with the working solution to form a solution according to the method described in US Pat. No. 7,115,301. A solution of a nucleic acid marker, by mixing a nucleic acid marker with a substrate that deposits a nucleic acid marker on the fibrous material, to produce a solution comprising a nucleic acid marker, for example, the nucleic acid marker can be mixed with water, and the solution containing the nucleic acid marker is then applied to A fiber or article to be marked, such as a textile material of fiber or fiber material. As a result of the present application, a labeled fibrous material can be produced by attaching a nucleic acid label to a fibrous material, such as but not limited to, the substrate can comprise an aqueous solvent, an adhesive, a polymer, a linker, or a cross-linking agent, The substrate may comprise acrylic acid, polyurethane, dimethyloldihydroxyethyleneurea, polyvinyl alcohol, starch, epoxy resin, or polyvinyl chloride.

According to an exemplary embodiment of the invention, the matrix may be optionally treated as a topical treatment of a fibrous material that can be mixed with a nucleic acid marker to produce a solution comprising a nucleic acid marker suitable for topical treatment of the fiber or article, a solution comprising the nucleic acid marker followed by The article can be applied topically to the article (e.g., fibrous material), and the labeled fibrous material can be produced by attaching the nucleic acid marker to the fibrous material. The matrix suitable for topical treatment comprises a colorant, a dye, a dyeing aid, a printing paste, a softener, a lubricant, an antistatic agent, a water repellent, an antibacterial agent, a wetting agent, a leveling agent, or water.

According to an exemplary embodiment of the present invention, the substrate may be a viscous fiber spinning solution for fiber spinning, and the viscous spinning solution may be mixed with a nucleic acid marker to form a viscous spinning dope containing a nucleic acid marker, the viscous property. The spinning dope is extruded through the opening of the spinneret to form the marked fibers, which are subsequently cured and can be used in any stage of the textile manufacturing process, such as cleaning, scouring, ginning, combing of the textile manufacturing process. Cotton, combed, roving, spinning, shaker, yarn guide, sizing, bale, guide, woven, braided and post-processed.

According to the above exemplary method, a solution containing a nucleic acid marker can be embedded in the fiber.

According to an exemplary embodiment of the invention, the nucleic acid can be mixed with a water insoluble matrix to form a solution comprising the nucleic acid marker. First, the nucleic acid is soluble in a water-soluble solution, and the method then dissolves the water-insoluble substrate in a solvent, and then the aqueous solution containing the nucleic acid marker is mixed with the non-aqueous substrate using an intermediate solution, and formed. The solution containing the nucleic acid marker is then used on the desired fiber or article, by way of example and not limitation, the vehicle solution used to prepare the solution containing the nucleic acid marker comprises an organic solvent such as ethanol, acetone, chloroform or other organic mixture.

Solutions containing nucleic acid markers can be deposited on textile fibers or articles during the textile manufacturing process. In the manufacture of textiles, there are several insertion points that can be used to deposit nucleic acid markers on textile materials. For example, solutions containing nucleic acid markers can be used on textile materials between or after scouring or ginning (see Figure 2 and in the following detailed description, the insertion points of a plurality of textile manufacturing processes will be explained in more detail below.

Application of nucleic acid markers on fibers or articles

In an exemplary embodiment of the invention, the fiber or article may comprise textiles, fibers Woven, cotton, raw cotton, ginned cotton, cotton blended, wool, yarn, Kashmir wool, synthetic fabrics and blended synthetic fabrics, such as any natural material, fiber or unprocessed material that can be treated with a nucleic acid label. The solution containing the nucleic acid marker can be used in fibers, yarns, sewing threads, cloth, non-woven materials, and any products made of cloth, such as textiles containing wool or cotton, which can be labeled to contain nucleic acid. Any consumer product that is solution treated.

In an exemplary embodiment of the invention, the solution comprising the nucleic acid marker can be dried on the fiber or article to be labeled, or absorbed by the material from which the article is made, for example, the fiber/article can be cotton or wool. The textile, and the solution containing the nucleic acid marker can be dried on the textile, and the solution containing the nucleic acid marker can be dried by any suitable drying process, such as air drying, drying, infrared drying, or ultraviolet drying. The fiber may be a natural or man-made material having a high aspect ratio, and has the property of being processed into a fabric, wherein the minimum composition of the fabric is similar to that of the natural world, and can be separated from the fabric, and the natural fiber can be used for it. It forms in the form of fibers when grown or grown, and may come from, for example, animal, plant, or mineral sources. Processed fibers (e.g., synthetic fibers) can be made from chemical compounds produced by a production plant, such as tantalum or nylon.

The yarn may be an assembly of fibers that are wound or laid together, thereby forming a continuous strand that can be made into a woven or woven fabric, which may be a woven fabric, a thread, or a material suitable for weaving, weaving, or Other continuous strands wound into textile fibers. The filament yarn can be made of processed fiber, except that a small proportion of the filament yarn is a filament, and the processed filament yarn can be extruded through the spinneret and solidified into a fiber form, and then with or without a silk thread. The monofilament yarn is made by agglomerating the monofilament yarn, and the spun yarn can be a continuous stranded wire that gathers the short fibers through a machine, such as a mechanical twisting machine, which combines the fiber by using the irregularity of the fiber and the natural cohesive force. Become a yarn together.

The sewing thread may be a yarn that is sewn together by mechanical or manual means, and the cloth may be a flexible sheet material composed of a solution, a fiber, a yarn, or any combination thereof. The fabric can be a soft, flat structure that can be made into two-dimensional or three-dimensional products that require some shape or softness. The fabric can be made from a wide variety of materials, such as solutions, fibers, yarns, and "composite" fibers. , fiberglass or carbon fiber. In the case of a fabric made of yarn, the fabric may be woven or woven fabric, and the woven fabric may be woven at right angles for more than two sets of yarns. Woven is a step of splicing a set of yarns into a fabric through a set of yarns, and the fabric made of the solution contains the polymer directly The solution is formed by melt extrusion or by casting a solution onto a film formed on a heat roller. A composite fabric is a fabric that combines a plurality of primary and/or secondary structures into a single structure, wherein at least one of the primary and/or secondary structures can be a recognized textile structure. Some cloths may be made directly from a fiber or fiber forming solution without the steps of making the yarn through the fibers. These non-woven constructions may comprise a fibrous sheet structure, made through a fibrous web, and mechanically wound, or The connection is made by adding a resin, a hot melt adhesive, or forming a chemical compound.

Those skilled in the art will appreciate that the systems and methods described above can be used to label fibers or articles, such as packaging materials, label materials, documents, and shipping containers, to determine their source, authenticity, or other supply chain or product information.

In an exemplary embodiment of the invention, a fiber or article labeled by a process is provided, the process may include providing a fiber/item, and placing the fiber/article on any suitable support surface to deposit a solution comprising the nucleic acid marker, for example. Said item can be placed on the surface of the substrate, platform, which can be a mobile platform or conveyor belt. A method of marking a fiber or article includes transporting the article in a direction of the transport mechanism in a direction along which the transport mechanism is located, the transport mechanism can include more than one nozzle. A method of marking a fiber or article can deposit a solution containing a nucleic acid marker on a fiber or article through more than one nozzle of the delivery mechanism, and an exemplary device for marking a fiber or article can be transmitted through Figures 3 and 4, Follow-up details.

In an exemplary embodiment, the textile production process can have multiple insertion points for nucleic acid markers that can be applied to treated or untreated fibers, or textiles at any stage during the textile production process (eg, raw wool) Or unprocessed textile material of raw cotton), or textiles of any stage in commercial circulation (for example, processed textiles through commercial circulation). The nucleic acid marker can be used as a solution containing a nucleic acid marker as described above, and an insertion point for a solution containing a nucleic acid marker provides a method of applying a solution containing a nucleic acid marker in the textile production process, during the textile production process, more than one The solution containing the nucleic acid marker can be added at more than one insertion point of the textile production process. For each manufacturer or process that uses the textile manufacturing process, a database can be maintained to store information about each nucleic acid sequence.

After an example insertion point is rolling, or cotton opening, and a cleaning process, the method is followed by a carding process step during which the staple fibers are pulled together into a slightly parallel arrangement to form a relative Weakly twisted into a rope-like fiber. The method continues with an additional combing step to produce a smooth, fine, uniform spun yarn made from long-staple fibers, the next step being a drawing step in which the processed fibers are stretched after the spinning step to change the molecular arrangement within the fibers, and during the roving process, the size of the drawn fibers is reduced, the fibers can become more parallel, and the introduction and a smaller amount of addition twist.

The second illustrative insertion point for the inclusion of the nucleic acid labeling solution is after the roving step, prior to spinning, the spinning may represent the step of making the yarn from unprocessed or staple fibers, and the spinning may also be referred to through the spinneret. The small holes squeeze the solution to make the fibers.

The third illustrative insertion point is that after spinning, the subsequent steps can be performed to form the original cotton cloth, and the original cotton cloth can be formed to include wetting, yarn guiding, singeing, merging, singeing, shaking, mercerizing, joining, and / or packing step, the guiding yarn refers to the step of transferring the yarn from one bundle to another, the singeing refers to the step of burning the end of the fiber to form a smooth surface, and the shaking yarn means removing the fiber and removing the yarn. The step of winding the fiber into a hank, which refers to a processing step in which sodium hydroxide is used in the step to increase the water absorption, gloss and/or strength of the cotton. After the original cotton cloth is produced, the method is followed by a step of performing a general/high temperature treatment in the step to remove proteins, waxes, lipids, and other impurities.

The fourth illustrative insertion point occurs after high temperature processing, prior to dyeing, which may refer to the addition of color to the illustrated textile manufacturing process. The textile may be made by using a dye or coating mixture, and the coating may contain a color-soluble particle affixed to the surface of the fiber by a bonding agent, the dye being an organic compound composed of a colored portion and comprising a site capable of bonding to the fiber, thus for the fourth insertion point, the nucleic acid marker It can be combined with a dye or coating mixture prior to contact with the textile.

After dyeing, the method is woven, and the weaving may refer to the step of forming the fibers by winding the yarns. The illustrative fifth insertion point occurs after weaving and before the cloth is dyed. The illustrative sixth insertion point occurs after the cloth is dyed. In the illustrated textile manufacturing process, the cloth dyeing process can be performed after weaving so that the woven textile can be dyed again, and the nucleic acid marking can be combined with the dye or coating mixture prior to deposition onto the textile. During the first three insertion points listed above, the solution containing the nucleic acid marker can be deposited directly onto the fiber or fiber material. As described above, the nucleic acid marker can be combined with a matrix to form a solution containing the nucleic acid marker, allowing the nucleic acid marker to be Attached to a fiber material or a product made of fiber material. The matrix may attach the nucleic acid label to a fibrous material, or a product made of a fibrous material, which matrix may, for example, comprise an alkaline activator.

At the fourth and fifth insertion points, the nucleic acid label can be deposited during the "processing" step, which can be a step for adding color and increasing the performance of the unfinished fiber, for example, in fibers, yarns. The steps performed before and after the manufacture of the thread, or fabric, to alter the appearance, texture, or feel of the marked fibers or articles.

The method for forming a solution comprising a nucleic acid marker deposited on a textile during a textile manufacturing process can be performed in a number of different manners. According to an exemplary embodiment, forming a solution comprising a nucleic acid marker can comprise mixing a unique nucleic acid sequence with a first matrix In the step, the first substrate can be liquefied in solution, and the solution comprising the nucleic acid marker can then be used on a textile which can be post-cured after evaporation of the solvent portion of the solution.

According to an exemplary embodiment of the present invention, the nucleic acid marker may be mixed with a water-insoluble substrate to form a solution containing a nucleic acid marker, the nucleic acid being first soluble in a water-soluble solution, followed by the water-insoluble matrix being soluble in the solvent, the medium The solution can then be used to mix a water soluble solution comprising a nucleic acid marker with a water insoluble matrix, and the resulting solution comprising the nucleic acid marker can then be used on a textile.

In accordance with an exemplary embodiment of the present invention, a method of depositing a nucleic acid marker on a fiber or article can include activating a nucleic acid marker and/or activating at least a portion of a surface of the article on which the nucleic acid marker is deposited. The nucleic acid marker can be activated alone, or the surface of the article can be activated, or both the nucleic acid marker and the surface of the fiber/article can be activated, for example, but not limited to, an activation site can be created on the nucleic acid marker, which can be associated with cellulose (cotton fiber, etc.) reaction, the activation site on the nucleic acid marker can also react with nylon, part of polyester, wool, or other fiber types.

2 illustrates a method for depositing a nucleic acid marker on a fiber or article, and a device configured to perform a method of depositing a nucleic acid marker on a fiber or article, in accordance with an exemplary embodiment of the present invention. Referring to FIG. 2, in accordance with an exemplary embodiment of the present invention, the apparatus for marking an article may be configured to use the nucleic acid marker on the unprocessed textile material by spraying a solution containing the nucleic acid marker during the ginning process. Processed textile materials (such as raw cotton fibers or unprocessed wool fibers). The apparatus for marking textiles can use a gin comprising more than one conveyor belt 211 in combination with more than one scouring tank 210, connecting the two in a string and configured to clean the unprocessed textile material. The apparatus for marking textiles can be configured to use a solution comprising a nucleic acid marker in a raw textile material during the scouring process of the unprocessed textile material, the raw textile material being any textile material such as raw wool. And raw cotton.

The apparatus for marking textiles can include a storage tub 220 that stores a solution containing nucleic acid markers, a pump 240, a metering controller 250, a transport mechanism 260, and more than one nozzle 270. The device can include an indicator 230 operatively coupled to the bucket 220 and configured to indicate when the stock of the solution containing the nucleic acid marker in the bucket 220 is low. The bucket 220 can be stored in a locked space 310 that is configured to record the time and identity of anyone entering the locked space.

The storage tub 220 can be of any desired size or volume to meet the amount required to hold the nucleic acid marker mixture, and the size of the storage tub 220 can be determined based on the desired amount of the nucleic acid-labeled mixture, for example, for continuous spraying of nucleic acid-containing markers. The solution is placed on the fiber or article for a desired period of time, and the size of the storage bin 220 can be selected; the size of the storage tank 220 can be determined based on the amount of unprocessed textile material to be marked. For example, storage tank 220 can be of any suitable size, such as but not limited to a 55 gallon tank configured to store a solution containing an acid label.

An indicator 230 coupled to the storage tank 230 and configured to indicate when the solution containing the nucleic acid marker in the bucket 220 is low may include a visual indicator such as a gauge, indicator or light, such as red light to display a nucleic acid marker The mixture is at a low stock, and green light may indicate that the nucleic acid marker mixture is not in a low inventory, in another example the yellow light may show the volume of the nucleic acid marker mixture in between.

The pump 240 can be coupled to the reservoir 220 and can be configured to draw a solution containing the nucleic acid marker from the reservoir 220 to the delivery mechanism 260, which can have any desired amount of the solution containing the nucleic acid marker pumped to the delivery The ability of the mechanism 260, for example, to deposit a specific amount of the solution containing the nucleic acid marker on the unprocessed textile material, depending on the desired flow rate, may select a suitable size of the pump 240, for example, the pump 240 may be configured to deliver A quantity of a solution comprising a nucleic acid marker to label an unprocessed textile material in an amount from 1 ng DNA per 1 kg textile to 1 μg DNA per 1 kg textile, and the pump 240 can be configured to extract a quantity of the solution containing the nucleic acid marker for labeling Raw textile materials, such as raw cotton or raw wool.

Both wool and cotton contain inherent water content, which contributes to the overall weight measurement of processed cotton produced in textile production. In the general production process of cotton and wool, the final water content of processed cotton or wool is maintained at an industrially acceptable level. For example, the length and strength of the drying step may increase or decrease the relative water content remaining after drying the cotton or wool. The water content of the processed cotton that has not been labeled with the solution containing the nucleic acid marker is usually maintained at the total weight of the cotton. about 8.5% w/w, while the water content of the processed wool that has not been labeled with a solution containing the nucleic acid marker is typically maintained at about 12% w/w of the total weight of the wool. In order to mark a raw textile material, such as cotton or wool, with a solution containing about 2% w/w of the nucleic acid mark in the total weight of the unprocessed textile material, the water content of the cotton or wool can be reduced by about 2% w/w. And a solution containing 2% w/w of the nucleic acid-labeled molecule in the total weight of the unprocessed textile material is replaced so as to maintain a standard cotton water content of 8.5% and a standard wool moisture content of 12%.

The flow rate of the pump 240 and/or the deposition rate of the transport mechanism 260 can be controlled by any suitable means, such as through the metering control unit 250, which can be placed at any desired location, such as the metering control unit 250. One or more nozzles 270 adjacent to or adjacent to the pump 240 are disposed. The metering control unit 250 can control the flow rate of the solution containing the nucleic acid marker to or through the delivery mechanism 260, and the metering control unit 250 can control the amount of the solution containing the nucleic acid marker discharged from one or more nozzles 270 of the delivery mechanism 260, thus, metering Control unit 250 can control the rate at which the solution containing the nucleic acid marker is deposited on the unprocessed textile material, and thus the amount of nucleic acid used to label the particular unprocessed textile material.

The delivery mechanism 260 can include more than one nozzle 270, and more than one nozzle 270 can be positioned anywhere along the delivery mechanism 260, for example, more than one nozzle 270 can be positioned to spray a solution containing the nucleic acid marker into a spray, overlaid on The scouring tank 210 is passing the unprocessed textile material of the gin or the scouring tank 210, and the scouring tank 210 can transport the unprocessed textile material to a high point along one or more inclined conveyor belts 211 and allow the raw textile material to fall into the scouring tank 210. Subsequent members, the process may be practiced as part of a scouring or ginned unprocessed textile material, and more than one nozzle 270 may be positioned to spray a solution containing the nucleic acid marker to the unprocessed textile material as it falls to the subsequent scouring tank 210 Unprocessed textile material.

According to an exemplary embodiment of the present invention, the nucleic acid marker solution can be withdrawn from the storage tank 220 and sent to the delivery mechanism 260, and the nucleic acid marker solution can be withdrawn to more than one nozzle 270 via the delivery mechanism 260, and the nucleic acid marker solution can then pass through more than one nozzle. 270 is sprayed onto the unprocessed textile material.

According to an exemplary embodiment of the present invention, a concentrated nucleic acid marker 120 and an alkaline activator 130 may be provided in stage 110, and in step 140, a concentrated nucleic acid marker 120 and an alkaline activator 130 may be added to the water, and then in the stage. The solution is stirred in 150 to form an activation solution containing the nucleic acid marker, and the activation solution containing the nucleic acid marker can then be transferred to the storage tank 220 for use, also A pre-activation solution comprising a nucleic acid marker can be provided in the reservoir 220.

Figure 3 illustrates a method of depositing a nucleic acid marker on a fiber or article, and a device configured to perform a method of depositing a nucleic acid marker on a fiber/article, in accordance with an exemplary embodiment of the present invention. Unless otherwise indicated, the apparatus and method described in FIG. 3 is substantially identical or similar to the apparatus and method discussed in FIG. Referring to Figure 3, in accordance with an exemplary embodiment of the present invention, the apparatus for marking fibers/items can be configured to spray a solution comprising a nucleic acid marker onto a processed textile material during the selection and cleaning of the unprocessed textile material. The solution containing the nucleic acid marker is applied to the unprocessed textile material. The device for marking textiles can be used in conjunction with a pick/cleaner 410 that includes more than one shipping belt 420, and can also be configured to provide picking/cleaning of the unprocessed textile material. The device for marking a textile material can be configured to apply a solution comprising a nucleic acid marker to a raw textile material during the picking/cleaning of the unprocessed textile material, the raw textile material being any textile material For example, wool or cotton.

The delivery mechanism 260 can include more than one spray bar 430 that secures more than one nozzle 270 of the delivery mechanism 260. The spray bar 430 can be suspended from a transport belt of the picker/washer 410, which can include a shade 440 that is configured to prevent a solution containing the nucleic acid marker from being sprayed onto an area other than the transport belt 420. The mask 440 can be of any desired size or area to prevent the solution containing the nucleic acid signature from being sprayed onto a region other than the conveyor belt 420. For example, the curtain 440 can be pyramidal in shape, including two extending from the boom A shielding element is formed under the angle.

According to an exemplary embodiment of the invention, the apparatus may include a manual sprayer 510 configured to be manually operated by a person standing along the conveyor belt 420, which may include an on/off switch or spray handle 520, It is configured to actuate a manual sprayer 510 that can be configured to spray a solution containing a nucleic acid marker at any point along the conveyor belt 420, the manual sprayer 510 can include a connection to the pump 240 and/or a metering controller A delivery hose 530 of 250, which may include a quick disconnect assembly 540 configured to connect or disconnect the delivery hose 530 of the manual sprayer 510 with a pump 240 or metering controller 250, the manual sprayer The 510 can also be directly connected to the storage tank 220 and can include its own pump and/or metering controller.

An exemplary embodiment of the present invention provides an apparatus for marking fibers or articles that includes a conveyor belt adapted to transport articles in the direction of the transport mechanism, the transport mechanism being disposed at a location along the conveyor belt. The conveyor belt can be any length, width, height, or other desired size to supply the item to be marked, the belt can be adapted to move in any desired direction, the transport The belt can be operated electrically or manually, the conveyor belt can transport items at a variety of speeds, the speed of the conveyor belt can be adjusted manually or automatically, the conveyor belt can be controlled by a computer system, the speed of which can be based, for example, on the flow rate, flow The pressure, or the deposition rate of the solution containing the nucleic acid marker, is adjusted.

The delivery mechanism can include more than one nozzle, the number of which can vary depending on, for example, the amount of solution containing the nucleic acid marker deposited on the fiber/item, the number of which can vary depending on the size or speed of the conveyor belt. The size of more than one nozzle can be adjusted individually and/or collectively to adjust, for example, flow rate, flow rate pressure, or deposition rate of a solution containing a nucleic acid marker. More than one nozzle is adjustable in position of the transport mechanism to move more than one nozzle. The direction in which more than one nozzle is facing is adjustable, such as adjusting the direction in which the solution containing the nucleic acid marker is expelled from more than one nozzle. The shape of more than one nozzle may be any suitable shape to expel a solution containing a nucleic acid marker, for example more than one nozzle may form a cone or a cylinder. More than one nozzle may be adapted to provide a spray of a solution comprising a nucleic acid marker on the material to be labeled. More than one nozzle may be adapted to provide continuous, discontinuous or intermittent spraying on the material to be marked.

The delivery mechanism can be adapted to deposit a solution comprising the nucleic acid marker onto the material by more than one nozzle and label the material with a solution comprising the nucleic acid marker. The transport mechanism can be placed at any suitable area along the conveyor belt, for example the transport mechanism can cover the width of the conveyor belt. The delivery mechanism can be placed at any desired angle to deposit a solution containing the nucleic acid marker on the material to be labeled, for example, the delivery mechanism can be suspended above the conveyor belt, or along one side of the conveyor belt. More than one transport mechanism can be placed at more than one location along the conveyor belt. The delivery mechanism can include more than one reservoir, and the reservoir can store a solution containing the nucleic acid marker.

In an exemplary embodiment of the invention, more than one nozzle may be disposed on a spray bar that is configured to deliver a solution comprising a nucleic acid marker to a fiber or article. The spray bar can be adapted to deposit a solution containing the nucleic acid marker on the fiber/item through more than one nozzle and mark the fiber and the article using a solution comprising the nucleic acid marker. The spray bar can be placed in any area of the conveyor belt platform. The spray bar can be placed at any desired angle to deposit a solution containing the nucleic acid signature on the fiber or article. More than one spray bar can be placed at more than one location along the conveyor belt. The spray bar is operatively coupled to more than one reservoir, and the reservoirs can store a solution containing a nucleic acid marker.

In an exemplary embodiment of the invention, the means for marking the fibers or articles may include a regulator (e.g., the metering controllers shown in Figures 3 and 4) in combination with the transport mechanism. The regulator can be adapted to adjust the amount of deposition of the nucleic acid labeling solution by means of more than one nozzle of the delivery mechanism. The adjuster can also be positioned at any desired location in conjunction with the delivery mechanism to adjust the amount of deposition comprising the nucleic acid labeling solution, for example, the regulator can be configured in accordance with the flow of the solution delivery delivery mechanism containing the nucleic acid label. The regulator can adjust, for example, the flow rate, flow pressure, or deposition rate of the nucleic acid labeling solution. The regulator can be adjusted manually or automatically. The regulator can be automatically regulated, for example by a computer system.

The regulator can adjust, for example, the flow rate, flow pressure, or deposition rate of the nucleic acid labeling solution at each of the separate nozzles or at all of the one or more nozzles simultaneously. The regulator can adjust the deposition rate of the nucleic acid labeling solution according to the speed of the conveyor belt, for example, if the conveyor belt moves at a slower speed, then the regulator can slow down the deposition rate of the nucleic acid labeling solution, for example, if the conveyor belt is Moving at a fast speed, the regulator then adjusts to contain the rate of deposition of the nucleic acid labeling solution to maintain the same speed as the conveyor belt. The adjuster can adjust the nucleic acid labeling solution to a suitable deposition rate depending on the number of fibers or articles on the conveyor belt.

The regulator can adjust the deposition rate of the nucleic acid labeling solution and adjust the amount of fiber or article deposited by the nucleic acid labeling solution (eg, aqueous solution) to achieve the desired water content concentration of the fiber/item, eg, not comprising nucleic acid The processed cotton labeled with the marking solution may have a water content concentration generally maintained at about 8.5% w/w of the total weight of the cotton, while the processed wool not labeled with the nucleic acid marking solution may have a water content concentration generally maintained at about the total weight of the wool. 12% w/w.

In an exemplary embodiment of the invention, the means for marking the fibers or articles may comprise a measuring device in combination with a delivery mechanism, the measuring device being adapted to measure the amount of solution comprising the nucleic acid marker deposited by the delivery device. The measuring device can be located at any desired location in conjunction with the delivery mechanism to measure the amount of nucleic acid labeling solution contained by more than one nozzle in the delivery mechanism, for example, the measuring device can be configured to discharge the delivery mechanism in accordance with the nucleic acid labeling solution. To set it up. The measuring device can measure, for example, the flow rate, the flow pressure, or the deposition rate of the nucleic acid labeling solution. The measuring device can measure, for example, the flow rate, the flow pressure or the deposition rate of the nucleic acid-containing marking solution through a separate spray head. The measuring device can be manually or automatically controlled and can be controlled by a computer system. The measuring device provides a signal to regulator that causes the regulator to adjust the deposition rate of the nucleic acid labeling solution. The measuring device provides a signal to regulator to adjust the package The rate at which the nucleic acid-containing labeling solution is deposited on the fiber or article to maintain the desired concentration of water. A computer system can be used to monitor or control the regulator and the measuring device.

Verification of marked items

Nucleic acid markers can be used to identify characteristics of fibrous materials, such as cotton or articles, which contain labeled fibrous materials, such as by determining whether an item of interest is labeled with a nucleic acid signature, and using the nucleic acid marker to determine the sense Whether the particular item of interest is true, for example but not limited to, the nucleic acid logo can be used for product information coding, such as the origin of the textile material, the source of the finished product, the manufacturer's message, the plant identification, the product identification, and any other required or relevant information. The nucleic acid marker is present on the fiber or article and can be identified or tracked at any point in commercial circulation.

The nucleic acid marker is present on the fiber or the article of interest and can be detected by a portable scanner or laboratory verification method, which may include PCR or isothermal amplification, and then any suitable specific A method for detecting a marker sequence, for example, by a specific amplicon size detection method or a specific marker sequence detection method which hybridizes to a sequence-specific probe. By way of example and without limitation, may be purchased from any commercial sources and / or test set, and laboratory verification portable scanner, for example, through New England BioLabs ^® Corporation (Ipswich, MA) .

According to an exemplary embodiment of the present invention, the identification data of each nucleic acid marker may be stored in a database, and the database may store a plurality of product information as described above.

In another embodiment, the wool can be marked by a method substantially similar to that described above. Wool obtained by shearing the hair of a sheep has a high content of lanolin (such as wool wax or wool oil), as well as dead skin, sweat residue, pesticides, and other substances such as soil or plants. Washing is carried out before the wool is used for commercial use as a step of washing the fatty wool. The scouring can be easily accomplished by an industrial step of soaking in a warm water bath or using a detergent or a base in a special apparatus.

In the test for labeling unprocessed wool in DNA (SigNature® T DNA, DNA Sciences, Inc., Stony Brook, New York), the alkali-activated double-stranded marker DNA was added to a scaled, filled 450 liters from a local source of water. The storage tank of unheated water is stirred and the aqueous DNA marker solution is mixed to obtain a concentration in the range of about 0.1 pg/ml to 1 μg/ml. The DNA is deposited on the unprocessed wool during the scouring step, and the 3000 kg batch of unprocessed wool (batch A) is separated from the whole bundle, and the loose fibers are passed through the spray after separation. A conveyor belt under the sprinkler that deposits the aqueous DNA marker solution from the scale tank at a rate of approximately 70 liters per hour and a target of 20 milliliters per kilogram of unprocessed wool. After the initial 45-60 minutes of spraying, the deposition rate of the aqueous DNA marker solution was estimated to be higher than the expected 20 ml per kg, so the aqueous DNA marker solution was replenished with 50 liters of water and remixed, about 120 liters after replenishment. The aqueous DNA marker solution is used to spray the remaining wool in 3000 kg of raw wool, and 50 portions of the sprayed raw wool are taken at intervals of two minutes and sequentially numbered A1 to A50, taken from each An aliquot of the sample was sent to Applied DNA Sciences, Inc., where the company performed PCR using two complementary primer pairs with the marker DNA, separating the amplified product by capillary electrophoresis and demonstrating detectability at all test time points. The labeled DNA was measured (see Figure 4, showing the results for every tenth sample in A1-A50).

By adding and mixing with water, the remaining supplemental aqueous DNA marker solution was again increased to a volume of 450 liters, and 200 liters of the second added aqueous DNA marker solution was deposited on the second batch, 5000 kg of raw wool (batch) Sub-B), 50 aliquots of sprayed raw wool are taken at intervals of five minutes, and sequentially numbered B1 to B50, taken from each aliquot of the sample for PCR as described above, by capillary electrophoresis The amplified product was isolated and displayed detectable marker DNA at all time points tested (see Figure 5, showing the results for samples of B1-B42).

By adding and mixing with water, the remaining secondary replenished aqueous DNA marker solution is again increased to a volume of 450 liters, and 200 liters of three added aqueous DNA marker solutions are used at a rate of about 50 liters per hour for deposition. On three batches, 25,000 kg of unprocessed wool, the first part of the third batch of unprocessed wool (batch C) was marked. After two hours of spraying, the aqueous DNA marker solution added three times was again added to 450 liters with water. And mixing to form four additional aqueous DNA marker solutions for continuous spray batch C, deposited on the raw wool at the same spray rate for 2 hours.

Adding the previously supplemented aqueous DNA marker solution to a volume of 450 liters, followed by spraying for 2 hours, then repeating the steps of replenishing, mixing, and spraying more than two times, followed by the final 450 liters for labeling batch B, For the remaining amount of 25,000 kg, 50 parts of the sprayed raw wool were taken at intervals of 15 minutes, and sequentially numbered as C1 to C50, and samples from each aliquot were subjected to PCR as described above. Capillary electrophoresis separates the amplified product and displays detectable marker DNA at all time points tested. Figure 6 shows the results of the C1-C42 sample, showing the fiber labeled with the most dilute marker DNA solution. Less detected signals are placed Big DNA.

Figure 7 shows verification data from processed and unprocessed textiles marked with the SigNature ^® T DNA. The X-axis shows the number of base pairs (BP), the Y-axis shows the relative fluorescence units (RFU), and in Figure 7, the shaded peaks in column A indicate that there are nucleic acids in the unprocessed textile. The presence of a marker (e.g., DNA), the shaded peak of column A indicates a PCR method by a nucleic acid marker (e.g., DNA of this example) that detects multiple copies of a particular amplicon that is copied. The shaded peaks in column B indicate that the processed textile contains the same nucleic acid marker as column A. The shaded peaks in columns A and B are substantially similar in size to the unshaded peaks, indicating nucleic acid markers in processed and unprocessed textiles. There are qualitatively similar quantities. Column C shows a set of negative controls in which the textiles were not labeled with nucleic acid markers and no peaks in column C represent undetected false positive results. Column D shows the positive control group of PCR. Compared with the peaks in column A and column B, the peak of column D is at different positions because the PCR positive control group has introduced different DNA sequences, which have columns A and B. The nucleic acid identified therein marks a different number of base pairs, but demonstrates that PCR signal amplification successfully replicates the amplicon of DNA in the control group, and the presence of peaks in column D indicates that the PCR reaction proceeds as expected. The missing peak line in column E is provided as a PCR negative control group, and further indicates that the PCR reaction is performed by relying on the DNA marker.

Figure 8 shows verification data from textile samples from DNA markers prepared substantially as described above before and after multiple dry cleaning cycles of the textile. The X axis represents the number of base pairs (BP) and the Y axis represents relative fluorescence units (RFU). In Fig. 8, the shaded peaks in column A indicate the presence of nucleic acid markers (such as DNA) detected in textiles before dry cleaning, and the shaded peaks in column B indicate the presence of nucleic acid markers after three cycles of dry cleaning, C The shaded peaks of the columns indicate the presence of a nucleic acid signature detected after five cycles of dry cleaning. The shaded peaks of columns B and C have substantially the same size and position, which indicates that in the samples tested in columns B and C, there are the same nucleic acid markers as in column A (ie, the same number is verified) The base pair nucleic acid), and the nucleic acid marker is substantially present in the same amount as column A, so dry cleaning does not reduce the number of nucleic acid markers on the labeled textile. Column D indicates the PCR positive control group, and the peaks in column D are at different positions than the peaks of A, B, and C. Because the PCR positive control group has introduced different DNA sequences, it has columns A, B, and C. The identified nucleic acid marks a different number of base pairs. The presence of a peak in column D represents that the PCR reaction proceeds as expected. The absence of a peak line in column E is provided as a negative PCR control and further indicates that the PCR reaction relies on the DNA marker.

Figure 9 shows validation data from capillary electrophoresis traces of PCR products from upholstery textiles substantially labeled with nucleic acid markers prepared as described above. X represents the number of base pairs (BP), and the Y axis represents relative fluorescence units (RFU). In Figure 9, the shaded peaks in column A indicate the presence of a nucleic acid marker (i.e., DNA) after the general processing of the sampled upholstery product in a commercially available upholstery. Column B indicates the presence of a nucleic acid marker in the unlabeled upholstery sample to provide a negative control, and no signal peak in column B indicates that no false positive was detected. Column C indicates the PCR positive control group, and the signal peak of column C is relative to the signal peak of column A. The position is at different positions because the PCR positive control group has introduced different DNA sequences, which have the nucleic acid markers identified in column A. Different numbers of base pairs. The signal peaks present in column C indicate that the PCR reaction proceeded as expected, and the missing signal peaks in column D were provided as a negative PCR control group and further indicated that the PCR reaction proceeded as expected.

Figure 10 shows the validation data from capillary electrophoresis traces of PCR products from carpets substantially labeled with nucleic acid markers prepared as described above. The X axis represents the number of base pairs (BP) and the Y axis represents relative fluorescence units (RFU). In Figure 10, the shaded peaks of column B indicate the presence of a nucleic acid marker (i.e., DNA) detected after the general processing of the sample of the carpet of the carpet was prepared. Column A represents a test for the presence of a nucleic acid marker in an unlabeled carpet to provide as a negative control. Column C represents the PCR positive control group, and the signal peak of column C is different from the signal peak position of column A because the PCR positive control group has introduced different DNA sequences, and the sequence is compared with that identified in column B. Nucleic acid markers with different numbers of base pairs. The presence of a signal peak in column C indicates that the PCR reaction proceeded as expected, and no signal peak in column D was provided as a PCR negative control group, and further indicated that the PCR reaction proceeded as expected. DNA labeling on wool also confirmed similar DNA labeling test results during the roving step, showing that the marker DNA of the length characteristic has a specific PCR amplicon product detection result. (The roving is a long, thin bundle of fibers produced during the spinning of wool, raw cotton and other fibers).

Similarly, testing of processed wool after DNA labeling and staining with a light blue dye produced a rapidly detectable characteristic PCR amplicon showing alkaline-activated DNA markers distributed throughout the processing of wool. It can be detected after the wool fiber or yarn has been processed. In addition, DNA markers can be further detected by PCR and capillary electrophoresis in fiber samples of finally produced carpet products and upholstery textiles made of DNA-labeled wool fibers or yarns.

DNA labeling of cotton in an air flow tube (leather tube) system

The DNA marker can be introduced at any step in the processing of cotton. For example, but not limited to, the DNA marker can be introduced in any of the following steps: washing, scouring, ginning, carding, combing, roving, spinning, shaking, and guiding. The final processing stage of the yarn, sizing, baling, guiding, woven, weaving or textile manufacturing process. In the embodiment shown in Fig. 11, the lint fiber is forced through a 3 inch diameter lint discharge tube through a pressurized air, and the DNA marker solution is diluted into water, wherein the water system is utilized in a reverse osmosis system. The 3 micron filter is obtained by filtration. An alkaline activated DNA marker solution containing a random sequence of pure natural vector DNA or artificial vector DNA, prepared at a concentration of 46 micrograms per liter as described above, from a 55 gallon (about 200 liter) storage tank or mixing tank to 33 The rate of cc/min is fed to a filtered water stream at a rate of 1.5 liters/min. The DNA solution diluted in the water stream is sprayed through a nozzle into a lint discharge tube, wherein the DNA: water droplets have an average diameter of less than 200 microns, most Good for 10-50 microns. A bundle of 25,000 pounds of lint that has been seeded by a cotton gin is blown into a 50 inch long, 3 inch diameter lint discharge tube at a rate of 16,000 cubic feet per minute through a gas stream. During this period, the DNA marker solution The droplets were thoroughly mixed in a vigorous forced air stream and the DNA marker solution was evenly applied to the lint fibers. The humidification of the water added through the lint allows the DNA marker solution to adsorb, replacing the water content lost by the lint during the heating and drying cycle. The lint is then compressed under compression/roller set to produce a continuous velvet-like lap which is then folded and compressed into a bundle of 500 pounds for sale. The DNA marker test confirmed the specific PCR amplification product of the marker DNA having the length characteristic, and the DNA marker for labeling the lint in the lint discharge tube was detected.

The various documents, patents, and published patent applications are hereby incorporated by reference in their entirety in their entirety in their entirety, It is intended to mean the definition provided by the present invention. While the invention has been shown and described with reference to the exemplary embodiments embodiments

110‧‧‧ stage

120‧‧‧nucleic acid marker

130‧‧‧Alkaline activator

140‧‧‧ stage

150‧‧‧ stage

220‧‧‧ storage bucket

230‧‧‧ indicator

240‧‧‧ pump

250‧‧‧Metric controller

260‧‧‧Transportation agency

270‧‧‧ nozzle

310‧‧‧Locked space

410‧‧‧Selection/Washing Machine

420‧‧‧Transportation belt

430‧‧‧Boom

440‧‧‧

510‧‧‧Manual sprinkler

520‧‧‧ Spray handle

530‧‧‧Transport hose

540‧‧‧Quick disconnect component

Claims (20)

A method of marking fibers, the method comprising: providing a plurality of fibers during a manufacturing process; depositing a marking solution on at least a portion of the fibers during the manufacturing process, wherein the depositing system utilizes a nozzle comprising more than one nozzle The transport mechanism performs; and thus marks the fibers. The method of marking a fiber according to claim 1, wherein the marking solution comprises a nucleic acid marker. The method of marking a fiber according to claim 1, wherein the nucleic acid marker comprises DNA. A method of labeling fibers according to claim 2, wherein the DNA is activated by a base or comprises a functional group. The method of marking fibers according to claim 1, wherein the manufacturing process is selected from the group consisting of washing, scouring, ginning, carding, combing, roving, spinning, shaking, island yarn, pulp. A group of yarns, bales, guides, weaving, weaving, and final processing. The method of marking fibers according to claim 1, wherein the fibers comprise a group selected from the group consisting of cotton, blended cotton, wool, yarn, nylon, Kashmir wool, synthetic fibers, and blended synthetic fibers. s material. The method of marking fibers according to claim 5, wherein the fibers comprise cotton fibers. The method of marking fibers according to claim 6, wherein the cotton fibers comprise raw cotton fibers. The method of marking fibers according to claim 6, wherein the cotton fibers comprise cotton fibers after ginning. The method of marking fibers according to claim 5, wherein the fibers are wool fibers, and a solution containing the nucleic acid marker is deposited on the wool fibers between or after the scouring process of the wool fibers on. The method of marking fibers according to claim 1, wherein the fibers are selected from a textile selected from the group consisting of single yarns, warp yarns, cloth, non-woven materials, and products made from a fiber material. The group formed. A device for marking fibers, the device comprising: a transport system adapted to transport fibers in a direction of a marking transport mechanism, the marking transport mechanism being disposed along the transport system; wherein the transport mechanism comprises more than one nozzle, adapted A solution containing the marker is deposited on at least a portion of the fibers by the one or more nozzles; and thus the fibers are labeled. The device for marking fibers according to claim 11, wherein the marker comprises a nucleic acid. The device for marking fibers according to claim 11 further comprising a metering controller configured to regulate the amount of solution deposited on the fibers comprising the nucleic acid marker. The device for marking fibers according to claim 13 wherein the nozzles are configured to deliver droplets of the solution comprising the nucleic acid marker in the delivery system. The device for marking fibers according to claim 11 wherein the delivery system comprises a piping system. A device for marking fibers according to claim 15 wherein the piping system comprises a gas flow piping system. A device for marking fibers according to claim 15 wherein the piping system is connectable to a cotton gin. A method of verifying a fibrous material, comprising: providing a plurality of fibers; depositing a nucleic acid marker on at least a portion of the fibers, the depositing being performed using a transport mechanism comprising more than one nozzle; producing the marked fibers; A sample of the fibers is labeled and the presence of the nucleic acid marker in the sample of the labeled fibers is analyzed; and thus the fibrous materials are judged to be true or counterfeit. The method of verifying a fibrous material according to claim 19, wherein the step of analyzing the presence of the nucleic acid marker in the sample of the fibers comprising the polymerase chain reaction reaction technique.