TW201643253A - Microfluid temperature controlling device and reaction platform thereof, and method of controlling microfluid temperature - Google Patents

Abstract

The present invention discloses a microfluid temperature controlling device. The microfluid temperature controlling device includes a reaction platform and a plurality of containers. The reaction platform has a first surface and a second surface opposite to each other. The reaction platform includes a fluid containing space, a plurality of containing portion, at least one inlet and at least one outlet. The fluid containing space disposes between the first surface and the second surface. Each containing portion has an opening and a tube wall, the opening disposes on the first surface, and the tube wall extends from the opening to the fluid containing space. The inlet and the outlet dispose on the outside of the fluid containing space. Each container contains fluid, the temperature of the fluid in different containers is different, and each container connects to the inlet and outlet. The present invention also discloses a microfluid reaction platform, and a method of controlling microfluid temperature.

Description

Microfluidic temperature control device and reaction platform thereof, and microfluid temperature control method

The present invention relates to a temperature control device, and more particularly to a microfluidic temperature control device.

In biotechnology-related experiments, it is often necessary to have a device that can assist in temperature control, the most common being a temperature control device for Polymerase Chain Reaction (PCR).

The polymerase chain reaction uses a small amount of deoxyribonucleic acid (DNA) and DNA polymerase to perform a specific replication amplification chain reaction in a wafer or a test tube to make a specific deoxyribonucleic acid. Fragments can be copied to the original 10 billion to 100 billion times, so that it is impossible to detect due to insufficient sample concentration. In other words, the polymerase chain reaction is often used in tests that can be quickly detected, such as screening for specific pathogens, or typing the infected virus.

The basic principle of the polymerase chain reaction is to first open the double-stranded structure of deoxyribonucleic acid (DNA) at a high temperature (such as 95 ° C) to form a single-strand form, which is generally referred to as denature. Next, the temperature is lowered to a temperature at which a primer can bind to a single strand of deoxyribonucleic acid (eg, 50 ° C), so that a primer can bind to a complementary single-stranded DNA, generally It is called annealing. Finally, the temperature is adjusted to a temperature suitable for the action of the deoxyribonucleic acid polymerase (for example, 72 ° C), so that the DNA polymerase can start from the primer and copy the corresponding DNA fragment, the reaction is generally called For extension. By constantly repeating cyclic denaturation, adhesion and prolonged reaction cycles, for example 35 reaction cycles were repeated to amplify a fragment of a particular DNA. Therefore, the polymerase chain reaction must allow the specific DNA fragments to be rapidly replicated during this cycle by precisely controlling the reaction temperature and the period of the reaction time.

In general, the temperature control device of the polymerase chain reaction has a reaction platform, which can accommodate a PCR tube or a PCR plate, and the temperature control device can regulate the temperature of the reaction platform, such as temperature rise. Or cool down. Therefore, the aforementioned DNA fragment, primer, deoxyribonucleic acid polymerase and reaction reagent are sealed in a micro tube, or sealed in a receiving element of the PCR plate, and the microtube or PCR plate is placed in the temperature control. In the reaction platform of the device, the reaction platform can be used to control the reaction temperature and the reaction time in the microtube or PCR plate.

However, the conventional temperature control device places the heat generating wafer and the heat dissipating component under the reaction platform, thereby quickly controlling the temperature of the reaction platform. However, there is still a limit to the rate at which the reaction platform is warmed and cooled. The rate of temperature rise is about 5 ° C / sec, and the rate of temperature drop is about 2 ° C / sec. As mentioned above, the polymerase chain reaction requires three temperatures. For each temperature adjustment, it takes about 30 seconds to wait for the temperature to rise and cool down. It must repeat about 35 reaction cycles, so performing a polymerase chain reaction requires at least a cost. About 1000 seconds or more, which is not conducive to rapid detection applications.

In view of the above problems, an object of the present invention is to provide a microfluidic temperature control device capable of reducing the time required for temperature rise and temperature reduction by a novel and simple design to achieve rapid temperature control.

To achieve the above object, a microfluidic temperature control device according to the present invention comprises a reaction platform and a plurality of accommodating elements. The reaction platform has a first surface and a second surface disposed opposite each other. The reaction platform includes a fluid accommodating space, a plurality of accommodating portions, at least one water inlet, and at least one water outlet. The fluid accommodation space is disposed between the first surface and the second surface. Each of the accommodating portions has an opening and a tube wall, and the opening is disposed on the first surface, and the tube wall extends from the opening to the fluid accommodating space. The water inlet and the water outlet are disposed outside the fluid accommodation space. Each of the accommodating components respectively accommodates fluids of different temperatures, and each of the accommodating components respectively communicates with the water inlet And the water outlet.

In an embodiment, the water inlet and the water outlet are disposed on opposite sides of the fluid accommodating space.

In one embodiment, the microfluidic temperature control device further includes a supply channel assembly and a recovery channel assembly. The supply channel assembly includes a first control valve, a main supply channel, and a plurality of sub-supply channels. The two ends of the main supply passage are respectively connected to the water inlet and the first control valve. The two ends of each of the sub-supply channels are respectively connected to the first control valve and each of the accommodating elements. The recovery channel assembly includes a second control valve, a primary recovery channel, and a plurality of sub-recovery channels. The two ends of the main recovery passage are respectively connected to the water outlet and the second control valve. The two ends of each of the sub-recovery passages are respectively connected to the second control valve and each of the accommodating elements.

In one embodiment, the reaction platform has a plurality of the water inlets and a plurality of the water outlets, and the microfluidic temperature control device further includes a supply channel assembly and a recovery channel assembly. The supply channel assembly has a plurality of supply channels, and the two ends of each of the supply channels are respectively connected to the water inlets and the plurality of receiving elements. The recovery channel assembly has a plurality of recovery channels, and the two ends of each of the recovery channels are respectively connected to the water outlets and the respective receiving components.

In one embodiment, the reaction platform is made of metal.

In an embodiment, the inner surface of the tube wall of each of the accommodating portions has a thermoplastic film.

In one embodiment, the microfluidic temperature control device further includes an additional reaction platform detachably disposed on the reaction platform, and the additional reaction platform has a plurality of microfluidic accommodating portions.

In an embodiment, the microfluidic temperature control device further includes at least one illuminating light source and at least one detecting unit. The illuminating light source is disposed on a side of the reaction platform adjacent to the second surface. The detecting unit is disposed on a side of the reaction platform adjacent to the first surface, and the detecting unit respectively corresponds to each opening.

In an embodiment, the microfluidic temperature control device further includes at least one illuminating light source and at least one detecting unit. The illuminating light source is disposed in the reaction platform. The detecting unit is disposed on a side of the reaction platform adjacent to the first surface, and the detecting unit respectively corresponds to each opening.

To achieve the above object, a microfluidic temperature control device according to the present invention includes a reaction platform, an additional reaction platform, and a plurality of accommodating elements. The reaction platform has a first surface and a second surface disposed oppositely, and the reaction platform includes a fluid accommodating space, at least a receiving portion, at least one water inlet and at least one water outlet. The fluid accommodation space is disposed between the first surface and the second surface. The accommodating portion has an opening, and the opening is disposed on the first surface. The water inlet and the water outlet are disposed outside the fluid accommodation space. The additional reaction platform is detachably disposed on the reaction platform, and the additional reaction platform has a plurality of microfluid accommodating portions respectively disposed at the respective openings. Each of the accommodating elements respectively accommodates fluids of different temperatures, and each of the accommodating elements is respectively connected to the water inlet and the water outlet.

In an embodiment, the reaction platform further has an elastic member, and the ring is disposed at the opening.

In one embodiment, the reaction platform further has an elastic member disposed on the outer side of the microfluidic receiving portion.

In one embodiment, the microfluidic temperature control device further includes an elastic member detachably disposed between the reaction platform and the additional reaction platform.

To achieve the above object, a microfluidic temperature control device includes a reaction platform and a plurality of accommodating elements. The reaction platform has a first surface and a second surface disposed opposite each other. The reaction platform includes a fluid accommodating space, a plurality of accommodating portions, at least one water inlet and one water outlet. The fluid accommodation space is disposed between the first surface and the second surface. Each of the accommodating portions has an opening and a tube wall, and the opening is disposed on the first surface, and the tube wall extends from the opening to the fluid accommodating space. The water inlet and the water outlet are disposed outside the fluid accommodation space. Each of the accommodating elements respectively accommodates fluids of different temperatures, and each of the accommodating elements is respectively connected to the water inlet.

To achieve the above object, a microfluidic temperature control device includes a reaction platform, an additional reaction platform, and a plurality of accommodating elements. The reaction platform has a first surface and a second surface disposed oppositely. The reaction platform includes a fluid receiving space, at least one receiving portion, at least one water inlet and a water outlet. The fluid accommodation space is disposed between the first surface and the second surface. The accommodating portion has an opening, and the opening is disposed on the first surface. The water inlet and the water outlet are disposed outside the fluid accommodation space. The additional reaction platform is detachably disposed on the reaction platform, and the additional reaction platform has a plurality of microfluid accommodating portions respectively disposed at the respective openings. Each of the accommodating elements respectively accommodates fluids of different temperatures, and each of the accommodating elements is respectively connected to the water inlet.

To achieve the above object, a microfluidic reaction platform according to the present invention has a first surface and a second surface disposed opposite thereto. The microfluidic reaction platform includes a fluid containing space Between, a plurality of accommodating portions, at least one water inlet and at least one water outlet. The fluid accommodation space is disposed between the first surface and the second surface. Each of the accommodating portions has an opening and a tube wall, and the opening is disposed on the first surface, and the tube wall extends from the opening to the fluid accommodating space. The water inlet and the water outlet are disposed outside the fluid accommodation space.

In an embodiment, the water inlet and the water outlet are disposed on opposite sides of the fluid accommodating space.

In one embodiment, the microfluidic reaction platform is made of metal.

In one embodiment, the inner surface of the tube wall of the receiving portion has a thermoplastic film.

In order to achieve the above object, a microfluidic temperature control method according to the present invention includes the following steps: providing a reaction platform including a fluid accommodating space and a plurality of accommodating portions, each of the accommodating portions having an opening and a tube wall extending from the opening to the fluid accommodating space; injecting a microfluid to the accommodating portion of the reaction platform; and injecting a first temperature fluid into the fluid accommodating space, the first temperature fluid and the tube wall portion Contacting; the first temperature fluid exits the fluid accommodation space; and injecting a second temperature fluid into the fluid accommodation space, the second temperature fluid contacting the tube wall portion, wherein the first temperature is different from the second temperature.

In order to achieve the above object, a microfluidic temperature control method according to the present invention includes the following steps: providing a reaction platform including a fluid accommodating space and a plurality of accommodating portions, each of the accommodating portions having an opening and a tube wall extending from the opening to the fluid receiving space; providing an additional reaction platform having a plurality of microfluidic receiving portions; at least one of the microfluidic receiving portions injecting a microfluid to the additional reaction platform One of the additional reaction platforms is placed on the reaction platform, and the microfluid accommodating portions are respectively disposed in the accommodating portions; the fluid at a first temperature is injected into the fluid accommodating space, and the fluid and the microfluid at the first temperature The receiving portion is partially in contact; the fluid at the first temperature is discharged from the fluid accommodating space; and the fluid in the second temperature is injected into the fluid accommodating space, and the fluid at the second temperature is in contact with the wall portion, wherein the first temperature and the second portion The temperature is not the same.

In order to achieve the above object, a microfluidic temperature control method according to the present invention comprises the steps of: providing a reaction platform comprising a fluid accommodating space, at least one accommodating portion, each having an opening; providing an additional a reaction platform having a plurality of microfluidic accommodating portions; at least one of the microfluid accommodating portions for injecting a microfluid to the additional reaction platform An additional reaction platform is placed on the reaction platform, and the microfluid accommodating portions are respectively disposed in the openings; a fluid of a first temperature is injected into the fluid accommodating space, and the first temperature fluid and the microfluid accommodating portion are Contacting; the first temperature fluid exits the fluid accommodation space; and injecting a second temperature fluid into the fluid accommodation space, the second temperature fluid contacting the tube wall portion, wherein the first temperature is different from the second temperature.

According to the microfluidic temperature control device, the microfluidic reaction platform and the microfluidic temperature control method of the present invention, the (microfluidic) reaction platform has a fluid accommodating space, and the tube wall of the accommodating portion is placed in the fluid. The space extension, in turn, the microfluidic temperature control device further has a plurality of accommodating elements for accommodating fluids of different temperatures. Therefore, the reaction platform can be quickly switched in temperature by injecting fluids of different temperatures in the fluid accommodation space of the reaction platform. And accommodating the microfluidic receiving portion, the wall of the tube extends in the direction of the second surface and is formed inside the fluid accommodating space, so that the microfluid inside the tube wall can reach the heat balance more quickly, so as to rapidly heat up And the effect of cooling.

In addition, compared with the conventional temperature control device, the microfluidic temperature control device of the present invention replaces the fluid of different temperatures through the fluid accommodating space of the reaction platform to regulate the temperature, thereby eliminating the setting of the heat generating chip and the heat dissipating component. Moreover, the size of the reaction platform can be greatly increased, thereby increasing the number of the receiving portions, and simultaneously increasing the number of reactions per experiment, which is helpful for a large number of testing experiments.

1, 1a, 1d, 1e, 1f, 1g, 1h, 1i, 1j, 1k, 1m, 1n‧‧‧ reaction platform

11, 11d, 11f, 11g, 11h, 11m‧‧‧ first surface

12, 12d, 12f, 12g, 12h, 12m‧‧‧ second surface

13, 13a, 13d, 13e, 13f, 13g, 13h, 13k, 13m, 13n‧‧‧ fluid accommodation space

14, 14a, 14d, 14e, 14f, 14g, 14h, 14i, 14j, 14k, 14m, 14n‧‧‧ housing

141, 141a, 141d, 141f, 141g, 141h, 141i, 141j, 141k‧‧

142, 142a, 142d‧‧‧ wall

143d‧‧‧ thermoplastic film

15, 15a, 15b, 15c, 15e, 15f, 15g, 15h, 15m, 15n‧‧‧ water inlet

16, 16a, 16b, 16c, 16e, 16f, 16g, 16h, 16m, 16n‧‧‧ water outlet

17‧‧‧ side wall

18h, 18i, 18j, 18k‧‧‧ elastic parts

2, 2a, 2e, 2f, 2g, 2h, 2i, 2j, 2k, 2m, 2n‧‧‧ accommodating components

21, 21a‧‧‧first accommodating components

22, 22a‧‧‧Second accommodating components

23, 23a‧‧‧ third receiving component

3, 3a, 3e, 3f, 3g, 3m, 3n‧‧‧ supply channel components

31, 31m‧‧‧ first control valve

31a, 31b, 31c‧‧‧ supply channels

32, 32m‧‧‧ main supply channel

33, 33m‧‧‧ sub-supply channel

331‧‧‧ first sub-supply channel

332‧‧‧Second sub-supply channel

333‧‧‧ third sub-supply channel

4, 4a, 4e, 4f, 4g‧‧‧ recycling channel components

41‧‧‧Second control valve

41a, 41b, 41c‧‧‧Recovery channels

42‧‧‧Main recovery channel

43‧‧‧Sub-recovery channel

431‧‧‧The first sub-recovery channel

432‧‧‧Second sub-recovery channel

433‧‧‧ third sub-recovery channel

5e, 5h, 5i, 5j, 5k, 5n‧‧‧Additional Reaction Platform

51e, 51h, 51i, 51j, 51k, 51n‧‧‧ microfluidic containment

6f, 6g‧‧‧ illuminating light source

7f, 7g‧‧‧detection unit

C‧‧‧ Groove

D1, D2, D3, D4, D5, D6, D7, D8‧‧‧ microfluidic temperature control device

1 is a schematic cross-sectional view showing a microfluidic temperature control device according to a first embodiment of the present invention.

2A is a schematic view of the reaction platform shown in FIG. 1.

2B is a schematic cross-sectional view of the reaction platform shown in FIG. 2A.

Figure 3 is a schematic illustration of the actuation of the microfluidic temperature control device of Figure 1.

4 is a schematic cross-sectional view showing a microfluidic temperature control device according to a second embodiment of the present invention.

Figure 5 is a schematic illustration of a reaction platform in accordance with a third embodiment of the present invention.

6A is an exploded perspective view of a microfluidic temperature control device according to a fourth embodiment of the present invention.

6B is a partially enlarged view showing the combination of the reaction platform and the additional reaction platform shown in FIG. 6A. intention.

Figure 7A is a partial schematic view of a microfluidic temperature control device in accordance with a fifth embodiment of the present invention.

Figure 7B is a partial schematic view of a microfluidic temperature control device in accordance with a sixth embodiment of the present invention.

Fig. 8A is a schematic cross-sectional view showing a microfluidic temperature control device according to a seventh embodiment of the present invention.

Figure 8B is a schematic view of the reaction platform shown in Figure 8A.

Fig. 9 is a schematic view showing another embodiment of the elastic member shown in Fig. 8A.

Figure 10 is a schematic view showing still another embodiment of the elastic member shown in Figure 8A.

Figure 11A is a schematic view showing still another embodiment of the elastic member shown in Figure 8A.

Figure 11B is an exploded perspective view of the microfluidic temperature control device shown in Figure 11A.

Figure 12 is a cross-sectional view showing a microfluidic temperature control device according to an eighth embodiment of the present invention.

Figure 13 is a cross-sectional view showing a microfluidic temperature control device according to a ninth embodiment of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, a microfluidic temperature control device and a microfluidic reaction platform according to a preferred embodiment of the present invention will be described with reference to the accompanying drawings, wherein the same elements will be described with the same reference numerals.

The microfluidic temperature control device of this embodiment is applied to a temperature control device for adjusting the temperature of the microfluid, and the microfluid referred to in this embodiment refers to a fluid having a volume between 0.1 and 1000 μL, such as a sample required for the experiment and Reaction reagent. The microfluid can be adjusted by the microfluidic temperature control device of the present embodiment to adjust the temperature of the microfluid itself, and then perform an experimental reaction such as a polymerase chain reaction (PCR) at a suitable ambient temperature. 1 is a schematic cross-sectional view of a microfluidic temperature control device according to a first embodiment of the present invention, which is first referred to FIG. The microfluidic temperature control device D1 of the present embodiment includes a reaction platform 1 and a plurality of accommodating members 2 for respectively accommodating fluids of different temperatures. This example is exemplified by a polymerase chain reaction, and it is known to those skilled in the art that the general polymerase chain reaction requires at least three different temperatures for denaturation and annealing. And an extension reaction step. Therefore, the microfluidic temperature control device D1 of the present embodiment has three accommodating elements 2, which may be better understood for the sake of explanation. In this embodiment, the accommodating elements 2 are referred to as a first accommodating element 21, respectively. Two accommodating elements 22 and third accommodating elements 23 for respectively accommodating three kinds of The fluids of the same temperature are referred to as the first temperature, the second temperature, and the third temperature, respectively, in this embodiment. The first accommodating member 21, the second accommodating member 22, and the third accommodating member 23 of the embodiment are thermostatic water tanks, so that the first accommodating member 21, the second accommodating member 22, and the third accommodating member can be disposed. The liquid inside the element 23 is maintained at a first temperature, a second temperature, and a third temperature, respectively. For example, the first temperature may be 95 ° C suitable for the denaturation reaction step and accommodated in the first accommodating member 21, and the second temperature may be 50 ° C suitable for the annealing reaction step and accommodated. The second accommodating member 22, and the third temperature may be 72 ° C suitable for the extension reaction step and housed in the third accommodating member 23. In addition, the first accommodating member 21, the second accommodating member 22, and the third accommodating member 23 have temperature-maintaining heating units, such that the first accommodating member 21, the second accommodating member 22, and the third accommodating member The internal fluid of 23 can be maintained at the first temperature, the second temperature, and the third temperature, respectively.

Of course, in other embodiments, the microfluidic temperature control device D1 can also be applied to other experiments that require temperature control, and the number of the required components can be adjusted according to the number of types of temperatures required for the experimental content. This is limited to this. For example, the temperature required for the annealing and extension reactions of a partial reverse transcription reaction experiment may be the same, for example, 50 ° C at the same time, so only two accommodating elements are needed at this time. 2. Of course, the invention does not limit the configuration of the receiving element 2, which can be configured as a trough, such as a sink, as shown in FIG. The accommodating member 2 can also be a bendable tubular structure, such as a coil, etc., and a space-saving effect can be achieved by a bent tubular configuration. The following embodiments are described by taking the accommodating member 2 of the tank as an example.

2A is a schematic view of the reaction platform shown in FIG. 1, and FIG. 2B is a schematic cross-sectional view of the reaction platform shown in FIG. 2A. Please refer to FIG. 2A and FIG. 2B simultaneously. The reaction platform 1 has a first surface 11 and a second surface 12 disposed oppositely. If the first surface 11 is referred to as a front surface, the second surface 12 is a bottom surface, and FIG. 2A is a second surface 12 indicated at the bottom. On the line, it refers to the plane (bottom) of the bottom. The reaction platform 1 includes a fluid accommodating space 13 , a plurality of accommodating portions 14 , at least one water inlet 15 , and at least one water outlet 16 . The fluid accommodating space 13 is disposed between the first surface 11 and the second surface 12, that is, formed inside the reaction platform 1. In detail, the reaction platform 1 of the present embodiment is composed of a first surface 11, a second surface 12 and four side walls 17, and the liquid accommodating space 13 is the first surface 11, the second surface 12 and the side walls. 17 Together form a closed cavity.

A plurality of accommodating portions 14 are arranged in sequence on the reaction platform 1. Each of the accommodating portions 14 has an opening 141 and a tube wall 142. The opening 141 is disposed on the first surface 11. The tube wall 142 extends from the opening 141 to the fluid accommodating space. The extension of the tube 13 extends from the opening 141 toward the second surface 12 such that the tube wall 142 is formed in the liquid accommodating space 13. And the tube wall 142 surrounds the opening 141 to form a closed space, and can accommodate the reagent or sample (ie, the aforementioned microfluid), in other words, when the reagent or sample is placed in the receiving portion 14, the liquid is placed in the liquid. Within space 13. Of course, during the experiment, the opening 141 is closed by a cover or a film to allow the microfluid to react in a closed space, which is a common knowledge in the art and will not be described herein.

The water inlet 15 and the water outlet 16 are disposed outside the fluid accommodating space 13, and may be disposed on the first surface 11, the second surface 12 or the side wall 17, and preferably, the water inlet 15 and the water outlet 16 are respectively disposed in the fluid The opposite sides of the accommodation space 13 are provided. The present invention does not limit the number of the water inlet 15 and the water outlet 16, and can adjust the number of the water inlet 15 and the water outlet 16 in accordance with the overall design of the fluid passage of the microfluidic temperature control device D1. This embodiment first uses a water inlet 15 and A water outlet 16 is provided on the opposite sides of the side wall 17, respectively. Of course, in other embodiments, the water inlet 15 and the water outlet 16 may be disposed on the first surface 11 or the second surface 12, and the invention is not limited thereto.

As shown in FIG. 1, each of the accommodating elements 2 is connected to the water inlet 15 and the water outlet 16, respectively, in other words, the first accommodating member 21, the second accommodating member 22, and the third accommodating member 23 are respectively connected to each other. The nozzle 15 and the water outlet 16 are provided. In detail, the microfluidic temperature control device D1 of the present embodiment further includes a supply channel assembly 3 and a recovery channel assembly 4, the supply channel assembly 3 communicates with the water inlet 15, and the recovery channel assembly 4 communicates with the water outlet 16. The supply channel assembly 3 includes a first control valve 31, a main supply passage 32, and a plurality of sub-supply passages 33. The two ends of the main supply passage 33 are respectively connected to the water inlet 15 and the first control valve 31, and each sub-supply The two ends of the passage 31 are connected to the first control valve 31 and the respective accommodating elements 2, respectively. In other words, the supply channel assembly 3 of the present embodiment communicates with the water inlet 15 through the main supply passage 32, and the plurality of sub-supply channels 33 communicate with the accommodating member 2, respectively, thereby passing the fluid in the accommodating member 2 via the main supply passage 32. The sub-supply channel 33 and the water inlet 15 are supplied to the fluid accommodating space 13 of the reaction platform 1.

Since the microfluidic temperature control device D1 of the present embodiment has three accommodating elements 2, the sub-supply channel 33 has three corresponding ones. For the sake of clarity of the description, the first sub-supply channel 331 and the second sub-supply are provided in this embodiment. The channel 332 and the third sub-supply channel 333 are illustrated as an example. In detail, one end of the first sub-supply channel 331, the second sub-supply channel 332, and the third sub-supply channel 333 are connected to the first control valve 31, and the other end of the first sub-supply channel 331 is connected to the first The other end of the second sub-supply channel 332 is connected to the second accommodating element 22, and the other end of the third sub-supply channel 333 is connected to the third accommodating element 23. The first control valve 31 can control the first sub-supply channel 331, the second sub-supply channel 332 or the third sub-supply channel 333 to communicate with the main supply channel 32. In other words, the first control valve 31 will be different in this embodiment. The temperature fluid (i.e., the fluid stored in the first accommodating member 21, the second accommodating member 22, or the third accommodating member 23) is supplied to the reaction platform 1, respectively.

Similarly, the flow channel structure of the recovery channel assembly 4 is substantially the same as that of the supply channel assembly 3. The recovery channel assembly 4 includes a second control valve 41, a main recovery channel 42 and a plurality of sub-recovery channels 43. This embodiment also has three sub-ports. The recovery channels 43 are respectively connected to the three accommodating elements 2, and are referred to as the first sub-recovery channel 431, the second sub-recovery channel 432, and the third sub-recovery channel 433, respectively. The two ends of the main recovery passages 42 are respectively connected to the water outlet 16 and the second control valve 41. The two ends of the sub-recovery passages 43 are respectively connected to the second control valve 41 and the respective accommodating elements 2, that is, the first sub-recovery passages. One end of the second sub-recovery channel 432 and the third sub-recovery channel 433 are connected to the second control valve 41, and the other end is respectively connected to the first accommodating member 21, the second accommodating member 22, and the third accommodating member. 23 connections. Therefore, the fluid injected into the interior of the fluid accommodating space 13 can be recovered into the corresponding accommodating member 2 by the main recovery passage 42 and transmitted through the control of the second control valve 41. In this embodiment, the fluid inside the first accommodating member 21, the second accommodating member 22, and the third accommodating member 23 is injected into the fluid accommodating space through a pump, and the fluid is also recovered by the pump. The use of the pump as a power source and its actuation in the first accommodating element 21, the second accommodating element 22, and the third accommodating element 23 are generally known in the art and will not be further described herein.

Hereinafter, the microfluidic temperature control device D1 of the present embodiment will be applied to the polymerase chain reaction as an example. First, in the denature reaction step, the first temperature (95 ° C) of the fluid in the first accommodating member 21 is controlled by the first control valve 31 through the first sub-supply. The passage 331, the main supply passage 32 and the water inlet 15 are injected into the fluid accommodating space 13, so that the fluid of the first temperature is at least partially in contact with the tube wall 142, as shown in FIG. 3, and FIG. 3 is the microfluid as shown in FIG. Schematic diagram of the operation of the temperature control device. At this time, the temperature of the reaction platform 1 is in thermal equilibrium with the fluid in the fluid accommodation space 13, the whole of the reaction platform 1 is raised to the first temperature (95 ° C), and the microfluids inside the tube wall 142 are available (sample and The reaction reagent) is subjected to a denature reaction. Then, the fluid of the first temperature in the fluid accommodating space 13 is recovered to the first accommodating member 21 via the water outlet 16 and the main recovery passage 42 and the first sub-recovery passage 431 by the control of the second control valve 41 (eg, Figure 1), and maintaining the fluid at the first temperature by the constant temperature function of the first accommodating member 21.

After the fluid of the first temperature is discharged from the fluid accommodating space 13, the control by the first control valve 31 is continued to pass the second temperature (50 ° C) of the fluid in the second accommodating member 22 through the second sub-supply channel. The 332 and the main supply passage 32 are injected into the fluid accommodating space 13 to raise the temperature of the reaction platform 1 to the second temperature to perform an annealing reaction. After the reaction is completed, the fluid is recovered to the second accommodating member 22 via the second sub-recovery passage 432 by the control of the second control valve 41. Similarly, the third temperature (72 ° C) of the fluid in the third accommodating member 23 is continuously injected into the fluid accommodating space 13 through the supply channel assembly 3 to perform an extension reaction, and after the reaction is completed, The main recovery passage 42 and the third sub-recovery passage 433 recover the fluid to the third accommodating member 23. The water inlet 15 and the water outlet 16 of the present embodiment may be respectively provided with valves to hold the fluid in the fluid accommodation space 13. Of course, the present invention does not limit the number of the accommodating elements 2, so in one embodiment, in addition to the aforementioned three accommodating elements 2 (ie, the first accommodating element 21, the second accommodating element 22, and the third accommodating element 23) In addition, a further accommodating element 2 can be added, and a fluid of about 4 ° C can be accommodated, which can be used to store the sample that has been completed. Specifically, after the extension reaction described above, a fluid at 4 ° C can be refilled to hold the sample that has been reacted.

Therefore, the microfluidic temperature control device D1 of the present embodiment allows the temperature of the tube wall 142 to be replaced by the fluid containing the different temperatures (the first temperature, the second temperature, and the third temperature) due to the fluid accommodating space 13 of the reaction platform 1. Quickly switch and cycle between the first temperature, the second temperature and the third temperature to achieve rapid heating and cooling. Specifically, the microfluidic temperature control device D1 of the present embodiment can quickly raise and lower the wall 142 by the principle of thermal balance. The temperature and the state in which the temperature of the reaction platform 1 and the temperature of the fluid inside the fluid accommodation space 13 reach a thermal equilibrium take only a short time. In other words, it takes only a few seconds for each temperature to be converted, so that the effect of rapid temperature rise and temperature drop can be achieved. For example, the microfluidic temperature control device D of the present embodiment can cool the reaction platform 1 from the first temperature (95 ° C) to the second temperature (50 ° C) in a short time, compared to the conventional temperature. The control device has a cooling rate of about 2 ° C / sec. It takes 22.5 seconds to cool the 95 ° C to 50 ° C. Therefore, the microfluidic temperature control device D1 of the present embodiment does have a high efficiency of temperature rise and fall. It can be seen from the above that the temperature rise and fall speed of the microfluidic temperature control device D1 of the present embodiment depends on the efficiency of the pump operation, so it is preferable to use an efficient and accurate pump to achieve high speed injection and The effect of recovering the liquid can accelerate the rate of temperature rise and fall.

In addition, in other embodiments, the supply channel assembly 3 and the recovery channel assembly 4 may also be in other embodiments, and only the reaction platform 1 must be in communication with the respective accommodating elements 2. 4 is a cross-sectional view showing a microfluidic temperature control device according to a second embodiment of the present invention, which is first referred to FIG. 4. The microfluidic temperature control device D2 of the present embodiment also has a reaction platform 1a, a plurality of accommodating members 2a, a supply channel assembly 3a, and a recovery channel assembly 4a. The reaction platform 1a has a plurality of water inlets 15a, 15b, 15c and a plurality of water outlets 16a, 16b, 16c, the number of which corresponds to the number of the accommodating elements 2a, so the microfluidic temperature control device D2 of the embodiment has The first accommodating member 21a, the second accommodating member 22a and the third accommodating member 23a, and the reaction platform 1a has three corresponding water inlets 15a, 15b, 15c and three water outlets 16a, 16b, 16c. Correspondingly, the supply channel assembly 3a of the present embodiment has a plurality of supply channels 31a, 31b, 31c, and the two ends of the respective supply channels 31a, 31b, 31c are respectively connected to the respective water inlets 15a, 15b, 15c and the respective accommodating elements 2a. (The first accommodating member 21a, the second accommodating member 22a, and the third accommodating member 23a). Similarly, the recovery passage assembly 4a has a plurality of recovery passages 41a, 41b, 41c, and the respective recovery passages 41a, 41b, 41c The two ends are respectively connected to the respective water outlets 16a, 16b, 16c and the respective accommodating elements 2a.

Specifically, the fluid accommodated at the first temperature in the first accommodating member 21a can be injected into the fluid accommodating space 13a via the supply passage 31a and the water inlet 15a to adjust the temperature of the reaction platform 1a to the first temperature. After the reaction is completed, the fluid is recovered to the first accommodating member 21a via the water outlet 16a and the recovery passage 41a. The fluid of the second temperature is via the supply passage 31b, The water inlet 15b is injected into the fluid accommodating space 13a, and is recovered to the second accommodating member 22a through the water outlet 16b and the recovery passage 41b. The fluid at the third temperature is injected into the fluid accommodating space 13a via the supply passage 31c and the water inlet 15c. It is recovered to the third accommodating member 23a through the water outlet 16c and the recovery passage 41c. The water inlets 15a, 15b, 15c and the water outlets 16a, 16b, 16c are respectively provided with valves, and are arranged with pumps to control fluids entering and leaving the fluid accommodation space 13a at different temperatures, thereby adjusting the overall reaction platform 1a. The temperature is used for the polymerase chain reaction, and the detailed operation can be referred to the microfluidic temperature control device D1, which will not be described herein.

The microfluidic temperature control device D1 of the first embodiment and the microfluidic temperature control device D2 of the second embodiment both regulate the reaction platform 1 by injecting fluids of different temperatures into the fluid accommodation space 13 (13a). The overall temperature, and each conversion of a temperature takes only a few seconds, so it can achieve rapid heating and cooling. Hereinafter, the microfluidic temperature control device D1 shown in FIG. 1 will be described together. Since the microfluids (samples and reagents) are placed inside the tube wall 142 and the tube wall 142 extends in the direction of the second surface 12, It is formed inside the fluid accommodation space 13. Therefore, the temperature at the tube wall 142 and the temperature of the fluid inside the fluid accommodating space 13 can reach the heat balance relatively quickly. Therefore, the present invention does not particularly limit the material of the reaction platform 1, and the design of the structure itself can achieve rapid temperature rise. And the effect of cooling.

In addition, the reaction platform 1 (1a) of the first and second embodiments may be a single plastic material, such as polypropylene (PP), and is a detachable and disposable reaction platform 1 (1a). . As shown in FIG. 1 and FIG. 2, before the experiment (polymerase chain reaction), one end of the main supply channel 32 can be fitted with the water inlet 15 and one end of the main recovery channel 42 can be fitted with the water outlet 16; Or as shown in FIG. 4, the supply passages 31a, 31b, and 31c are respectively engaged with the water inlets 15a, 15b, and 15c, and the recovery passages 41a, 41b, and 41c are respectively fitted with the water outlets 16a, 16b, and 16c for performing. The aforementioned action of injecting fluids of different temperatures. After the experiment is completed, the reaction platform 1 (1a) can be directly removed to achieve a detachable and disposable effect. In other embodiments, the same is a detachable and disposable reaction platform, the material of which can also be metal material, composite plastic material, such as teflon and plastic (PP) composite material, or can be plastic Composite material with metal.

FIG. 5 is a schematic diagram of a reaction platform according to a third embodiment of the present invention. Please refer to FIG. 5. This embodiment is also a detachable and disposable reaction platform 1d, wherein the embodiment The reaction platform 1d is a composite material of plastic and metal. Specifically, the overall structure of the accommodating portion 14d and the tube wall 142d thereof are metal, such as stainless steel, tinplate, aluminum or copper. In this embodiment, aluminum is taken as an example, and the accommodating portion 14d is formed by press forming. Its tube wall 142d. The inner surface of the tube wall 142d of the receiving portion 14d has a thermoplastic film 143d to form a composite material of plastic and metal. Wherein, the thermoplastic film 143d may extend to the first surface 11d of the reaction platform 1d or even to the second surface 12d. In detail, the overall material of the reaction platform 1d can make the reaction platform 1d have better heating and cooling efficiency, and the inner surface of the tube wall 142d is coated with the thermoplastic film 143d, which can avoid microfluidics (samples and reagents) ) react with metals to avoid errors in experimental data. The thermoplastic film 143d may be, for example but not limited to, polyethylene, teflon, which has good ductility and can be bonded to the metal to be formed on the inner surface of the tube wall 142d.

6A is an exploded perspective view of a microfluidic temperature control device according to a fourth embodiment of the present invention, and FIG. 6B is a partially enlarged schematic view showing the combination of the reaction platform and the additional reaction platform shown in FIG. 6A. Please refer to FIG. 6A and FIG. 6B simultaneously. Show. In this embodiment, the reaction platform 1e may be non-disposable, and the material thereof is preferably a metal material with better thermal conductivity (refer to the foregoing), and the reaction platform 1e has at least one accommodating portion 14e. A plurality of accommodating portions 14e will be described as an example. The microfluidic temperature control device D3 further includes an additional reaction platform 5e. Preferably, the additional reaction platform 5e can be made of plastic or metal, and can be detachably disposed on the reaction platform 1e, and the additional reaction platform 5e is used. Can be abandoned directly. The additional reaction platform 5e has a plurality of microfluid accommodating portions 51e for accommodating the sample and the reagent (microfluid), and the configuration of the microfluid accommodating portion 51e corresponds to the accommodating portion 14e, so that the additional reaction The platform 5e can be directly placed on the reaction platform 1e, and each of the microfluid accommodating portions 51e is provided in each of the accommodating portions 14e, and the additional reaction platform 5e is heated through the reaction platform 1e. Therefore, after the experiment (polymerase chain reaction) using the microfluidic temperature control device D3 of the present embodiment, the additional reaction platform 5e can be directly replaced without disassembling the reaction platform 1e from the supply channel assembly 3e and the recovery channel assembly 4e. Untie it.

7A is a partial schematic view of a microfluidic temperature control device according to a fifth embodiment of the present invention, as shown in FIG. 7A. The microfluidic temperature control device D4 of the present embodiment further includes at least one illuminating light source 6f and at least one detecting unit 7f. In this embodiment, a plurality of illuminating light sources 6f and a plurality of detecting units 7f are taken as an example for illustration. In this embodiment, if the second surface of the reaction platform 1f 12f is a light transmissive material, such as the single plastic or composite plastic material, the illuminating light source 6f is disposed on the side of the reaction platform 1f close to the second surface 12f, and the number of the illuminating light sources 6f of the embodiment corresponds to the reaction platform. The accommodating portion 14f of 1f has a corresponding illuminating light source 6f for each accommodating portion 14f. Of course, in other embodiments, only one illuminating light source 6f may be provided, and other optical components, such as a light guide plate, may be used to uniformly illuminate the light to each of the accommodating portions 14f, and the microfluid of the embodiment may be completed. Temperature control device D4. The detecting unit 7f is a photodiode and is disposed on a side of the reaction platform 1f close to the first surface 11f. In other words, the detecting unit 7f is disposed on a side of the reaction platform 1f corresponding to the opening 141f, and each detecting unit 7f corresponds to each opening 141f of the reaction platform 1f, respectively. By the arrangement of the illuminating light source 6f and the detecting unit 7f, the microfluidic temperature control device D4 of the embodiment can be applied to an instantaneous quantitative polymerase chain reaction (QPCR) for detecting fluorescence, for each capacity. The microfluidics (samples and reagents) in the portion 14f are subjected to fluorescence quantification.

Of course, in other embodiments, the microfluidic temperature control device D4 can also be used as a detecting unit 7f and used in combination with a stepping motor. The detecting unit 7f is driven by the stepping motor to respectively correspond to the openings of the respective receiving portions 14f. The 141f performs the detection, and the microfluidic temperature control device D4 of the embodiment can also be completed. In addition, the detecting unit 7f can also be an optical fiber and connected to a photomultiplier (PMT), and the optical signal detecting unit 7f guides the optical signal to the photomultiplier tube for subsequent quantitative analysis.

7B is a partial schematic view of a microfluidic temperature control device according to a sixth embodiment of the present invention, as shown in FIG. 7B. The reaction platform 1g of the microfluidic temperature control device D5 of the present embodiment is a material that is opaque. For example, if the metal material is used, the illuminating light source 6g can be processed by a suitable waterproofing process, and then the illuminating light source 6g is set in the reaction. Within the platform 1g to complete the microfluidic temperature control device D5. For details of the other components of the microfluidic temperature control device D5, for example, the detecting unit 7g can be used with a stepping motor, or it can also be an optical fiber, etc., refer to the fifth embodiment, and details are not described herein.

According to the conventional fluorescent quantitative temperature control device, since the conventional temperature control device places the heat generating chip and the heat dissipating component under the reaction platform, the illuminating light source and the detecting unit can only be disposed on the reaction platform. On one side of the opening (ie, above the reaction platform), and each of the accommodating portions must have an illuminating light source and a detecting unit, thereby making each accommodating portion The spacing is limited by the size of an illumination source and a detection unit. The microfluidic temperature control device D4 of the fourth embodiment is configured to dispose the illuminating light source 6f and the detecting unit 7f on the side of the reaction platform 1f adjacent to the second surface 12f and the first surface 11f, respectively, or the microfluid of the fifth embodiment. The temperature control device D5 is provided with the light-emitting source 6g and the detecting unit 7g in the reaction platform 1g and on the side close to the first surface 11g, respectively. Therefore, the spacing of each of the accommodating portions 141f (141g) is limited only by the size of one detecting unit 7f (7g), so that the spacing between each of the accommodating portions 141f (141g) can be greatly shortened, and the capacity can be greatly increased. The number of sets 141f (141g) is set, and the number of reactions per experiment can be greatly increased.

8A is a schematic cross-sectional view of a microfluidic temperature control device according to a seventh embodiment of the present invention, and FIG. 8B is a schematic view of the reaction platform shown in FIG. 8A. Please refer to FIG. 8A and FIG. 8B simultaneously. The microfluidic temperature control device D6 of the present embodiment includes a reaction platform 1h, a plurality of accommodating elements 2h, and an additional reaction platform 5h. The connection relationship between the reaction platform 1h and the accommodating component 2h can be referred to the foregoing embodiment, and details are not described herein. The reaction platform 1h of the present embodiment also has a first surface 11h and a second surface 12h disposed oppositely, and the reaction platform 1h also includes a fluid accommodating space 13h disposed between the first surface 11h and the second surface 12h, and The water inlet 15h and the water outlet 16h disposed on the outer side of the fluid accommodating space 13h, and the manner in which the water inlet 15h and the water outlet 16h communicate with the accommodating member 2h can be referred to the first embodiment, and can be applied to the second embodiment as well. Example of the way of communication.

Each of the accommodating portions 14h of the reaction platform 1h of the present embodiment has an opening 141h, and the opening is disposed on the first surface 11h, and the microfluidic temperature control device D6 of the embodiment further includes an additional reaction platform 5h, which is detachable. Set on the reaction platform 1h. The additional reaction platform 5h has a plurality of microfluid accommodating portions 51h for accommodating the reagents or samples, and the microfluid accommodating portions 51h are respectively disposed at the respective openings 141h, so that the fluid accommodating space 13h forms a closed space, so when the fluid The fluid accommodating space 13h is injected, and there is no problem of overflow, and the fluid can be in direct contact with the microfluid accommodating portion 51h, that is, contact with the tube wall of the microfluid accommodating portion 51h, so as to achieve the effect of rapid temperature rise and temperature drop. In this embodiment, the material of the reaction platform 1h may be a metal having a better heat conduction effect, and the material of the additional reaction platform 5h may be a single plastic material with a lower cost or a composite material of metal and plastic. It can be discarded directly after use to achieve a disposable effect. Preferably, the reaction platform 1h further has an elastic member 18h, and the ring is disposed at the opening 141h. In order to form a structure in which the elastic member 18h is combined with the reaction platform 1h, when the additional reaction platform 5h is placed on the reaction platform 1h, the sealing effect between the microfluid accommodating portion 51h and the opening 141h can be increased by the elastic member 18h.

FIG. 9 is a schematic view showing another embodiment of the elastic member shown in FIG. 8A, which is shown in FIG. 9. In the present embodiment, the elastic member 18i is disposed on the additional reaction platform 5i, and is disposed on the outer side of the microfluid accommodating portion 51i, that is, the elastic member 18i is disposed on the outer side of the tube wall of the microfluid accommodating portion 51i. To form a structure in which the elastic member 18i is combined with the additional reaction platform 5i. Moreover, the elastic member 18i is disposed adjacent to the opening of the microfluid accommodating portion 51i, and when the additional reaction platform 5i is placed on the reaction platform 1i, the elastic member 18i can be engaged with the periphery of the opening 141i of the accommodating portion 14i. Achieve the effect of sealing.

In addition, FIG. 10 is a schematic view showing still another embodiment of the elastic member shown in FIG. 8A, which is shown in FIG. In the present embodiment, the elastic member 18j is not composited with the reaction platform 1j, and is not composited with the additional reaction platform 5j, but is detachably disposed between the reaction platform 1j and the additional reaction platform 5j. In other words, in use, it is sandwiched between the reaction platform 1j and the additional reaction platform 5j, that is, the elastic member 18j is directly sandwiched between the reaction platform 1j and the additional reaction platform 5j. It should be particularly noted that the elastic member 18j shown in FIG. 10 has a three-dimensional structure for the sake of clarity, and the elastic member 18j is also disposed in the opening 141j of the reaction platform 1j.

11A is a schematic view showing still another embodiment of the elastic member shown in FIG. 11A, and FIG. 11B is an exploded perspective view of the microfluidic temperature control device shown in FIG. 11A. As shown in FIGS. 11A and 11B, the elasticity of the embodiment is shown in FIG. The piece 18k is also not composited with the reaction platform 1k, nor is it compounded with the additional reaction platform 5k, but is detachably disposed between the reaction platform 1k and the additional reaction platform 5k. In this embodiment, the reaction platform 1k has a receiving portion 14k, and the receiving portion 14k has an opening 141k, and the additional reaction platform 5k is disposed on the opening 141k. The elastic member 18k of the present embodiment may be a rubber band-like structure, disposed between the reaction platform 1k and the additional reaction platform 5k, and close to the periphery of the reaction platform 1k or the additional reaction platform 5k to achieve the sealing effect. As shown in FIG. 11B, the reaction platform 1k and the additional reaction platform 5k can form a groove C for accommodating the elastic member 18k, so that the reaction platform 1k and the additional reaction platform 5k are sealed by the elastic member 18k. effect. Of course, in other embodiments, only the reaction platform 1k has the groove C, or the additional reaction platform 5k has the groove C, and the elastic member 18k can be detachably arranged. The technical means between the reaction platform 1k and the additional reaction platform 5k.

Figure 12 is a cross-sectional view showing a microfluidic temperature control device according to an eighth embodiment of the present invention, as shown in Fig. 12. The microfluidic temperature control device D7 of the present embodiment also includes a reaction platform 1m, and a plurality of accommodating members 2m for respectively accommodating fluids of different temperatures. The reaction platform 1m has a first surface 11m and a second surface 12m disposed opposite to each other, and includes a fluid accommodating space 13m, at least one accommodating portion 14m, at least one water inlet 15m, and a water outlet 16m. In the foregoing embodiment, the fluid accommodating space 13m is disposed between the first surface 11m and the second surface 12m, and the water inlet 15m and the water outlet 16m are disposed outside the fluid accommodating space 13m. For details of the detailed technical features of the accommodating portion 14m, reference may be made to the foregoing, and no further details are provided herein.

In this embodiment, the accommodating elements 2m are respectively connected to the water inlet 15m, and the communication mode may be as shown in FIG. 11. The microfluidic temperature control device D7 may further include a supply channel assembly 3m to communicate with the water inlet 15m. As with the supply channel assembly 3 of the first embodiment, the supply channel assembly 3m of the present embodiment also includes a first control valve 31m, a main supply passage 32m, and a plurality of sub-supply channels 33m, thereby enabling the water inlet 15m to be associated with each of the receiving components. 2m is connected, and the fluid in each of the accommodating elements 2m can be injected into the fluid accommodating space 13m through the first control valve 31m. In the present embodiment, the reaction platform 1k may have only one water outlet 16m to directly discharge the fluid inside the fluid accommodation space 13m, and is not recovered to the accommodating member 2m. Each of the accommodating elements 2m can be respectively connected to a supply of fluid of different temperatures to obtain fluids of different temperatures. Of course, in other embodiments, the reaction platform may have a plurality of water inlets and a plurality of supply channels to communicate with a plurality of receiving elements. Referring to the reaction platform 1a and the supply channel assembly 3a of the second embodiment, the present invention Not limited to this.

Of course, the aforementioned fluid is directly discharged from the inside of the fluid containing space, and is not recycled to the design of the accommodating member, and can also be applied to the aspect of the microfluidic temperature control device D3 of the fourth embodiment. Referring to FIG. 13, FIG. 13 is a schematic cross-sectional view of a microfluidic temperature control device according to a ninth embodiment of the present invention. The microfluidic temperature control device D8 of the present embodiment also includes a reaction platform 1n, and a plurality of accommodating devices. The component 2n respectively accommodates fluids of different temperatures, and the microfluidic temperature control device D8 further has an additional reaction platform 5n detachably disposed on the reaction platform 1n. The detailed technical features of the reaction platform 1n and the additional reaction platform 5n can be referred to the reaction platform 1e and the additional reaction platform 5e of the aforementioned fourth embodiment. Similarly, the additional reaction platform 5n There are a plurality of microfluid accommodating portions 51n, and are respectively disposed in the accommodating portion 14n.

In this embodiment, the accommodating elements 2n are respectively connected to the water inlet 15n, and the manner of communication can be referred to the eighth embodiment. Briefly, the microfluidic temperature control device D7 can further include a supply channel assembly 3n. To communicate with the water inlet 15n. In the present embodiment, the reaction platform 1n may have only one water outlet 16n to directly discharge the fluid inside the fluid accommodation space 13n, and is not recovered to the accommodating member 2n. Each of the accommodating elements 2n can be respectively connected to a supply of fluid of different temperatures to obtain fluids of different temperatures. Similarly, in other embodiments, the reaction platform may have a plurality of water inlets and a plurality of supply channels to communicate with a plurality of accommodating elements. Referring to the reaction platform 1a and the supply channel assembly 3a of the second embodiment, The invention is not limited to this.

The present invention further provides a microfluidic reaction platform having a first surface and a second surface disposed oppositely. The microfluidic reaction platform includes a fluid receiving space, a plurality of receiving portions, at least one water inlet, and at least one Water nozzle. The fluid accommodation space is disposed between the first surface and the second surface. The accommodating portion has an opening and a tube wall, and the opening is disposed on the first surface, and the tube wall extends from the opening to the fluid accommodating space. The water inlet and the water outlet are disposed outside the fluid accommodation space. The microfluidic reaction platform is a reaction platform for accommodating the microfluid. Therefore, the specific implementation and variations of the microfluidic reaction platform can be referred to the reaction platform of the foregoing embodiments, and will not be further described herein.

The present invention further provides a microfluidic temperature control method, comprising the steps of: providing a reaction platform comprising a fluid accommodating space and a plurality of accommodating portions, each of the accommodating portions having an opening and a tube wall, respectively The wall extends from the opening to the fluid receiving space; a microfluid is injected into the receiving portion of the reaction platform; and a fluid of a first temperature is injected into the fluid receiving space, and the fluid at the first temperature is in contact with the wall portion. For details of the operation, refer to the implementation method of the microfluidic control device of the first embodiment, and no further details are provided herein.

The present invention further provides a microfluidic temperature control method, comprising the steps of: providing a reaction platform comprising a fluid accommodating space and a plurality of accommodating portions, each of the accommodating portions having an opening and a tube wall, respectively The wall extends from the opening to the fluid receiving space; an additional reaction platform is provided having a plurality of microfluidic receiving portions; at least one of the microfluidic receiving portions injecting a microfluid to the additional reaction platform; The reaction platform is placed on the reaction platform, and the microfluid accommodating portions are respectively disposed in the accommodating portions; the fluid at a first temperature is injected into the fluid Accommodating space, the first temperature fluid is in contact with the microfluid accommodating portion; the first temperature fluid is discharged from the fluid accommodating space; and the second temperature fluid is injected into the fluid accommodating space, and the second temperature is fluid and the tube The wall portion is in contact, wherein the first temperature is different from the second temperature. For details of the operation, refer to the implementation method of the microfluidic control device of the seventh embodiment, and no further details are provided herein.

The invention further provides a microfluidic temperature control method, comprising the steps of: providing a reaction platform comprising a fluid accommodating space, at least one accommodating portion, the accommodating portion having an opening; and providing an additional reaction platform having a plurality of microfluidic accommodating portions; at least one of the microfluid accommodating portions for injecting a microfluid to the additional reaction platform; the additional reaction platform is placed on the reaction platform, and the microfluid accommodating portions are respectively disposed at the respective The opening; injecting a fluid of a first temperature into the fluid accommodating space, the fluid of the first temperature is in contact with the portion of the microfluid accommodating portion; the fluid of the first temperature is discharged from the fluid accommodating space; and the fluid of the second temperature is injected to the fluid The fluid housing space, the second temperature fluid is in contact with the tube wall portion, wherein the first temperature is different from the second temperature. For details of the operation, refer to the implementation method of the microfluidic control device of the seventh embodiment, and no further details are provided herein.

In summary, according to the microfluidic temperature control device, the microfluidic reaction platform and the microfluidic temperature control method of the present invention, the (microfluidic) reaction platform has a fluid accommodating space, and the tube wall of the accommodating portion is placed in the fluid. The space extension, in turn, the microfluidic temperature control device further has a plurality of accommodating elements for accommodating fluids of different temperatures. Therefore, the reaction platform can be quickly switched in temperature by injecting fluids of different temperatures in the fluid accommodation space of the reaction platform. And accommodating the microfluidic receiving portion, the wall of the tube extends in the direction of the second surface and is formed inside the fluid accommodating space, so that the microfluid inside the tube wall can reach the heat balance more quickly, so as to rapidly heat up And the effect of cooling.

In addition, compared with the conventional temperature control device, the microfluidic temperature control device of the present invention replaces the fluid of different temperatures through the fluid accommodating space of the reaction platform to regulate the temperature, thereby eliminating the setting of the heat generating chip and the heat dissipating component. Moreover, the size of the reaction platform can be greatly increased, thereby increasing the number of the receiving portions, and simultaneously increasing the number of reactions per experiment, which is helpful for a large number of testing experiments.

The above is intended to be illustrative only and not limiting. Any equivalent modifications or changes made to the spirit and scope of the present invention should be included in the attached application. In the range of interest.

1‧‧‧Reaction platform

11‧‧‧ first surface

12‧‧‧ second surface

13‧‧‧Fluid accommodation space

14‧‧‧ 容 部

141‧‧‧ openings

142‧‧‧ wall

15‧‧‧ water inlet

16‧‧‧Water outlet

17‧‧‧ side wall

2‧‧‧Receiving components

21‧‧‧First accommodating element

22‧‧‧Second accommodating element

23‧‧‧ Third accommodating element

3‧‧‧Supply channel components

31‧‧‧First control valve

32‧‧‧Main supply channel

33‧‧‧sub-supply channel

331‧‧‧ first sub-supply channel

332‧‧‧Second sub-supply channel

333‧‧‧ third sub-supply channel

4‧‧‧Recycling channel components

41‧‧‧Second control valve

42‧‧‧Main recovery channel

43‧‧‧Sub-recovery channel

431‧‧‧The first sub-recovery channel

432‧‧‧Second sub-recovery channel

433‧‧‧ third sub-recovery channel

D1‧‧‧Microfluidic temperature control device

Claims (22)

A microfluidic temperature control device comprising: a reaction platform having a first surface and a second surface disposed oppositely, the reaction platform comprising: a fluid accommodating space disposed on the first surface and the second surface Each of the plurality of accommodating portions has an opening and a tube wall, the opening being disposed on the first surface, the tube wall extending from the opening to the fluid accommodating space; and at least one a nozzle and at least one water outlet disposed outside the fluid receiving space; and a plurality of accommodating members, each of the accommodating members respectively accommodating fluids of different temperatures, and each of the accommodating members is respectively connected to the Inlet and the outlet. The microfluidic temperature control device of claim 1, wherein the water inlet and the water outlet are disposed on opposite sides of the fluid accommodation space. The microfluidic temperature control device of claim 1, further comprising: a supply channel assembly, comprising: a first control valve; a main supply channel, the two ends of which are respectively connected to the water inlet and the first a control valve; and a plurality of sub-supply channels, wherein the two ends of the sub-supply channels are respectively connected to the first control valve and each of the accommodating components; and a recovery channel assembly, comprising: a second control valve; The recovery channel has two ends connected to the water outlet and the second control valve, and a plurality of sub-recovery channels, and the two ends of each of the sub-recovery channels are respectively connected to the second control valve and each of the accommodating components. The microfluidic temperature control device of claim 1, wherein the reaction platform has a plurality of the water inlets and a plurality of the water outlets, and the microfluidic temperature control device further comprises: a supply channel assembly having a plurality of a supply channel, the two ends of each of the supply channels are respectively connected to each of the water inlets and the plurality of receiving components; and a recovery channel component having a plurality of recycling channels, wherein the two ends of the recycling channels are respectively connected to Each of the water outlets and each of the receiving elements. The microfluidic temperature control device according to claim 1, wherein the reaction platform is made of metal. The microfluidic temperature control device of claim 5, wherein the inner surface of the tube wall of each of the accommodating portions has a thermoplastic film. The microfluidic temperature control device of claim 1, further comprising: an additional reaction platform, detachably disposed on the reaction platform, the additional reaction platform having a plurality of microfluidic receiving portions. The microfluidic temperature control device of claim 1, further comprising: at least one illuminating light source disposed on a side of the reaction platform adjacent to the second surface; and at least one detecting unit disposed on the reaction platform The detecting unit respectively corresponds to each of the openings on a side close to the first surface. The microfluidic temperature control device of claim 1, further comprising: at least one illuminating light source disposed in the reaction platform; and at least one detecting unit disposed on the reaction platform adjacent to the first surface On the side, the detecting units respectively correspond to the respective openings. A microfluidic temperature control device comprising: a reaction platform having a first surface and a second surface disposed oppositely, the reaction platform comprising: a fluid accommodating space disposed on the first surface and the second surface At least one accommodating portion, the accommodating portion has an opening, the opening is disposed on the first surface; and at least one water inlet and at least one water outlet are disposed outside the fluid accommodating space; an additional reaction a platform, detachably disposed on the reaction platform, the additional reaction platform has a plurality of microfluid accommodating portions respectively disposed in each of the openings; and a plurality of accommodating components, each of the accommodating components The components respectively accommodate fluids of different temperatures, and each of the receiving components is respectively connected to the water inlet and the water outlet. The microfluidic temperature control device of claim 10, wherein the reaction platform further has an elastic member, and the ring is disposed at the opening. The microfluidic temperature control device of claim 10, wherein the reaction platform further has an elastic member disposed on an outer side of the microfluidic receiving portion. The microfluidic temperature control device of claim 10, further comprising: an elastic member detachably disposed between the reaction platform and the additional reaction platform. A microfluidic temperature control device comprising: a reaction platform having a first surface and a second surface disposed oppositely, the reaction platform comprising: a fluid accommodating space disposed on the first surface and the second surface Each of the plurality of accommodating portions has an opening and a tube wall, the opening being disposed on the first surface, the tube wall extending from the opening to the fluid accommodating space; and at least one a nozzle and a water outlet disposed outside the fluid accommodating space; and a plurality of accommodating members, each of the accommodating members respectively accommodating fluids of different temperatures, and each of the accommodating members is respectively connected to the water inlet . A microfluidic temperature control device comprising: a reaction platform having a first surface and a second surface disposed oppositely, the reaction platform comprising: a fluid accommodating space disposed on the first surface and the second surface At least one accommodating portion, the accommodating portion has an opening, the opening is disposed on the first surface; and at least one water inlet and one water outlet are disposed outside the fluid accommodating space; an additional reaction platform Removably disposed on the reaction platform, the additional reaction platform has a plurality of microfluid accommodating portions respectively disposed in each of the openings; and a plurality of accommodating members, each of the accommodating members The fluids of different temperatures are respectively accommodated, and each of the accommodating components is respectively connected to the water inlet. A microfluidic reaction platform having a first surface and a second surface disposed oppositely, the microfluidic reaction platform comprising: a fluid accommodating space disposed between the first surface and the second surface; Each of the accommodating portions has an opening and a tube wall, and the opening is disposed at The first surface, the tube wall extends from the opening to the fluid accommodating space; and at least one water inlet and at least one water outlet are disposed outside the fluid accommodating space. The microfluidic reaction platform of claim 16, wherein the water inlet and the water outlet are disposed on opposite sides of the fluid accommodating space. The microfluidic reaction platform described in claim 16 is made of metal. The microfluidic reaction platform of claim 18, wherein the inner surface of the tube wall of each of the accommodating portions has a thermoplastic film. A microfluidic temperature control method includes the following steps: providing a reaction platform comprising a fluid accommodating space and a plurality of accommodating portions, each of the accommodating portions having an opening and a tube wall respectively The opening extends toward the fluid accommodating space; injecting a microfluid to the accommodating portion of the reaction platform; injecting a first temperature fluid into the fluid accommodating space, the first temperature fluid is in contact with the tube wall portion; The first temperature fluid is discharged into the fluid accommodation space; and a second temperature fluid is injected into the fluid accommodation space, the second temperature fluid is in contact with the tube wall portion, wherein the first temperature and the first The two temperatures are not the same. A microfluidic temperature control method includes the following steps: providing a reaction platform comprising a fluid accommodating space and a plurality of accommodating portions, each of the accommodating portions having an opening and a tube wall respectively Opening an opening to the fluid receiving space; providing an additional reaction platform having a plurality of microfluidic receiving portions; injecting a microfluid to at least one of the microfluidic receiving portions of the additional reactive platform; An additional reaction platform is disposed on the reaction platform, and the microfluid accommodating portions are respectively disposed in each of the accommodating portions; and a fluid of a first temperature is injected into the fluid accommodating space, the first temperature fluid and the microfluid The receiving portion is partially in contact; The first temperature fluid is discharged into the fluid accommodation space; and a second temperature fluid is injected into the fluid accommodation space, the second temperature fluid is in contact with the tube wall portion, wherein the first temperature and the first The two temperatures are not the same. A microfluidic temperature control method comprising the steps of: providing a reaction platform comprising a fluid accommodating space, at least one accommodating portion, the accommodating portion having an opening; and providing an additional reaction platform having a plurality of micro a fluid receiving portion; injecting a microfluid into at least one of the microfluid accommodating portions of the additional reaction platform; the additional reaction platform is placed on the reaction platform, and the microfluid accommodating portions are respectively disposed at each The opening; injecting a first temperature fluid into the fluid accommodating space, the first temperature fluid is in contact with the microfluid accommodating portion; the first temperature fluid is discharged from the fluid accommodating space; and injecting a first The fluid of the second temperature is in the fluid accommodating space, and the fluid of the second temperature is in contact with the wall portion of the tube, wherein the first temperature is different from the second temperature.