TW201631147A - Manufacturing method and use of nutritional composition for anaerobic microorganisms to buffer pH value of water - Google Patents

Abstract

A manufacturing method and a use of a nutritional composition for anaerobic microorganisms to buffer pH value of water are provided. The nutritional composition mainly contains organic nutritional substrate and further contains Mg(OH)2 which is mixed with fatty acids of oils in the organic nutritional substrate to react and form magnesium salts of fatty acids and glycerol. Both of the magnesium salts of fatty acids and glycerol can be used as a long-acting buffering substance to provide a long-term pH buffering function for releasing OH<SP>-</SP>. Thus, when the nutritional composition is injected into the underground water for accelerating in-situ anaerobic microorganisms to degrade chlorinated organic contaminants, the nutritional composition can efficiently neutralize organic acids generated by anaerobic reactions of the anaerobic microorganisms, and maintain the neutral pH environment of the underground water for a long time, so that it is suitable for the anaerobic microorganisms to grow well.

Description

Anti-microbial nutrient substrate buffering water pH value and use thereof

The present invention relates to a pH-reducing microbial nutrient matrix for buffering water body and its use, and particularly relates to a nutrient matrix which can be utilized by a buffer substance produced by magnesium hydroxide for buffering the pH value of the groundwater body. And its use.

All microorganisms have their own pH environment that is most suitable for growth. Although the acidophilic (alkali) bacteria have been screened out in low (high) pH environments, most of the microorganisms are suitable for neutral environment (pH value). 6-8), the neutral pH is beneficial to enhance the efficiency of microbial growth reaction. For example, many important microflora that can be subjected to reductive dechlorination are also known to be suitable for neutral growth environments, such as Dehalococcoides dehalogenating bacteria, in which the growth pH of a dehalogenating group capable of degrading ethylene and tetrachloroethylene is known. Between 6.8-7.5. Similarly, the optimum pH range for several Desulfitobacterium dehalogenated desulfurous bacteria groups that degrade vinyl chloride and ethane is also near neutral. Therefore, maintaining a stable pH environment is an important part of promoting dechlorination.

The reductive dechlorination reaction of anaerobic microorganisms is an acidogenic reaction, which must have sufficient pH buffering material to maintain the reaction. In a natural water environment, the pH buffer system is usually a carbonic acid/dicarbonate system. When the reductive dechlorination reaction is carried out, the production of hydrochloric acid causes the bicarbonate to form carbon dioxide (carbonic acid), which is not easily dissipated into the atmosphere and remains in the water. :HCl+HCO ₃ ^- → H ₂ CO ₃ +Cl ^-

The decrease in bicarbonate concentration and the production of carbonic acid are accompanied by a decrease in pH: H ₂ O+CO ₂ → H ₂ CO ₃ → H ⁺ + HCO ₃ ^-

In addition, when most organic matter is fermented to produce hydrogen, acetic acid is also formed and the pH is lowered: CH ₃ COOH → H ⁺ +CH ₃ COO ^-

In the original groundwater environment, the pH value is usually not easy to change, and most of the pH changes are caused by, for example, biodegradation to produce acidic substances, local chemical oxidant injection, and electron donor injection into the environment. In the past, in order to improve the buffer capacity of groundwater, it is usually necessary to inject a highly soluble matrix or an alkali (acid) substance to increase the alkali (acid) degree of groundwater, but it cannot be stably maintained for a long time. Therefore, in the general anaerobic biological rehabilitative method of groundwater, in addition to the in-situ microbial culture with the infusion of the organic substrate, if there is a pH adjustment problem, an additional pH buffering agent is required.

In the current groundwater anaerobic bioremediation method, the most common pH adjusting agents are sodium hydroxide (NaOH) and potassium hydroxide (KOH). In other engineering fields, the two are often used for pH adjustment. Pharmacy. However, sodium hydroxide and potassium hydroxide are safe for workers in the application. Improper application may cause corrosive occupational injuries. In addition, improper use of dose control may cause the pH of the aquifer to be too high, which is not conducive to Microbial reaction; in addition, the precipitation of hydroxide complex in the underground aquifer will also cause the well screen obstruction and water permeability of the groundwater well; and the long-term use of sodium hydroxide or potassium hydroxide will also cause sodium or potassium ions in the groundwater. The high concentration leads to doubts about the salinization of the groundwater layer and the soil layer, which may pose a health hazard to residents who are inadvertently taking water downstream. On the other hand, sodium carbonate (Na ₂ CO ₃ ) and sodium bicarbonate (NaHCO ₃ ) can be used to provide low alkalinity acid-base buffering capacity in groundwater, but because of the low alkalinity supply, the amount must be It is improved, but after a long period of heavy use, the sodium ion concentration is also high, causing the water layer and the soil layer to be salinized.

Therefore, it is necessary to provide an anaesthetic microbial nutrient substrate capable of buffering the pH value of a water body to solve the problems of the above conventional techniques.

The main object of the present invention is to provide a anaerobic microbial nutrient matrix for buffering water body pH value, and the anatrophic microbial nutrient matrix is further added with magnesium hydroxide (Mg(OH) ₂ ) based on organic anaerobic substances. By mixing magnesium hydroxide with organic anaerobic fatty acids to form fatty acid magnesium salts and glycerol, and using fatty acid magnesium salts and glycerin as long-acting buffer substances to provide long-term slow release alkalinity pH buffering capacity, it is possible to inject groundwater. Inducing local anaerobic microorganisms (such as Dehalococcoides sp. ) to effectively neutralize organic acids produced by anaerobic microbial reactions when degrading chlorinated organic pollutants in water, and maintaining the most suitable anaerobic microbial reaction in water for a long time. pH environment.

In order to achieve the above object, the present invention provides a anaerobic microbial nutrient substrate for buffering water body pH, comprising 46 to 55 parts by weight of vegetable oil, 0.46 to 0.5 parts by weight of magnesium hydroxide (Mg(OH) ₂ ), 3.7. ~5 parts by weight of biodegradable surfactant, 2.3 to 4 parts by weight of vitamins and 46 to 50 parts by weight of water; in the anatrophic microbial nutrient matrix, magnesium hydroxide is mixed with vegetable oil fatty acid to form fatty acid magnesium salt and Glycerin, the anatrophic microbial nutrient matrix uses fatty acid magnesium salts and glycerin as long-acting buffers to provide a long-term pH buffering capacity.

In an embodiment of the invention, the anatrophic microbial nutrient substrate comprises 46 parts by weight of vegetable oil, 0.46 parts by weight of magnesium hydroxide, 3.7 parts by weight of biodegradable surfactant, 2.3 parts by weight of vitamins and 46 parts by weight. Water.

In one embodiment of the invention, the anatrophic microbial nutrient substrate is in the form of an oil droplet suspension in which a plurality of oil droplet particles are suspended.

In one embodiment of the invention, the fatty acid magnesium salt and glycerin are mixed in a plurality of oil droplets of the oil droplet suspension.

In one embodiment of the invention, the oil droplet particles have a particle size ranging from 1-2 [mu]m (micrometers).

In an embodiment of the invention, the vitamin is, for example, a vitamin B group.

In one embodiment of the invention, the vitamin B population comprises primarily vitamin B12.

In one embodiment of the invention, the biodegradable surfactant is selected from the group consisting of lipophilic surfactants or hydrophilic surfactants.

In one embodiment of the invention, the lipophilic surfactant is selected from the group consisting of lecithin.

In one embodiment of the invention, the hydrophilic surfactant is selected from the group consisting of butyl cellosolve or sapindus extract saponin.

Furthermore, the present invention provides another anaerobic microbial nutrient matrix for buffering water pH, which comprises vegetable oil, magnesium hydroxide, biodegradable surfactant, vitamins and water, wherein magnesium hydroxide occupies the nutrient matrix of the anatrophic microorganism The total weight is 0.46~0.5wt%; in the anatrophic microbial nutrient matrix, the magnesium hydroxide is mixed with the fatty acid of the vegetable oil to form the fatty acid magnesium salt and the glycerin, and the anatrophic microbial nutrient matrix is long-acting with the fatty acid magnesium salt and glycerin. Buffer material to provide a long-term pH buffering capacity.

In addition, the present invention provides a use of a buffered water body pH value of an anatomic microbial nutrient substrate, which is injected into a groundwater aquifer via a groundwater well to promote local anaerobic microorganisms to degrade groundwater. a chlorinated organic contaminant, wherein the anatrophic microbial nutrient substrate uses a fatty acid magnesium salt and glycerin as a long-acting buffer material to provide a long-term pH buffering capacity for effectively neutralizing the organic acid produced by the anaerobic microbial reaction, and Long-term maintenance of the water is most suitable for anaerobic microbial reactions in a neutral pH environment.

In one embodiment of the invention, the chlorine-containing organic contaminant is trichloroethylene.

In an embodiment of the present invention, the representative strain of the anaerobic microorganism is Dehalococcoides sp .

The above and other objects, features, and advantages of the present invention will become more apparent from

According to a preferred embodiment of the present invention, the present invention provides a anaesthetic microbial nutrient substrate for buffering water pH value, and the main use thereof is applied to the field of green remediation technology of biological rejuvenation method to provide anatrophic microorganisms. The nutrient source required for growth, used for stimulating (bio-stimulation) the number of native protozoa, thereby accelerating the efficiency of biodegradable organic pollutants, achieving environmental friendliness, green remediation and biological rejuvenation, etc., the anatrophic microorganism of the present invention The nutrient substrate can effectively neutralize the organic acids produced by the anaerobic microbial reaction when the in situ anaerobic microorganisms (such as Dehalococcoides sp. ) degrade the chlorinated organic pollutants in the water, and maintain the most suitable water in the water for a long time. The oxygen microbial reaction neutralizes the neutral pH environment to enhance the biodegradability of the groundwater target pollutants and to alleviate the problem of soil acidification caused by anaerobic reaction. The above organic pollutants are, for example, chlorine-containing organic pollutants in dense non-aqueous phase liquids (DNAPL), especially vinyl chloride (VC), dichloroethylene (DCE), Trichloroethylene (TCE), tetrachloroethylene (PCE), dichloromethane or chloroform, but not limited thereto. The above-mentioned green remediation mainly refers to the remediation of groundwater contaminated, for example, the remediation of groundwater contaminated by DNAPL (especially VC, DCE, TCE).

In the application of anaerobic biological rehabilitative methods for groundwater, in-situ microbial culture is often carried out by infusion of organic substrates. If pH adjustment is required, additional buffering agents are required. The anaesthetic microbial nutrient substrate of the present invention is mainly applied to a local groundwater environment with poor buffering capacity, and the anatrophic microbial nutrient substrate of the present invention is additionally added with magnesium hydroxide (Mg(OH) ₂ based on organic anaerobic materials). Therefore, the hydroxide ion can be directly buffered, and the magnesium salt and the glycerin can be formed by mixing the fatty acid in the organic anaerobic substance with magnesium hydroxide, and further using the fatty acid magnesium salt and glycerin as another Long-acting buffer material for long-term slow release of alkalinity (such as OH ^- ) during remediation of the groundwater environment, thus having a long-term buffering capacity to maintain the neutral pH environment most suitable for anaerobic microbial reactions in water, ie When an organic acid is released by an anaerobic reaction of an anaerobic microorganism (such as Dehalococcoides sp. ) to release an organic acid, the organic acid produced by the anaerobic reaction is neutralized to maintain a neutral pH environment for a long period of time.

C ₃ H ₅ (COOC ₁₇ H ₃₅ ) ₃ +3Mg(OH) ₂ → 3C ₁₇ H ₃₅ COOMg+C ₃ H ₅ (OH) ₂

In more detail, the anatrophic microbial nutrient substrate of the present invention comprises: 46 to 55 parts by weight of vegetable oil, 0.46 to 0.5 parts by weight of magnesium hydroxide (Mg(OH) ₂ ), and 3.7 to 5 parts by weight of a biodegradable interface. An active agent, 2.3 to 4 parts by weight of vitamins and 46 to 50 parts by weight of water, and the above ingredients are sufficiently mixed and have an oil droplet suspension in which a plurality of oil droplet particles are suspended. That is, the vegetable oil and the biodegradable surfactant (and vitamin) form oil droplets (ie, oil droplets) uniformly dispersed and dispersed in liquid water, and the fatty acid magnesium salt formed by mixing magnesium hydroxide with vegetable oil oil and glycerin.

In the anatrophic microbial nutrient matrix of the present invention, the vegetable oil may be selected from the group consisting of soybean oil, peanut oil, sunflower oil, canola oil, olive oil, palm oil, bitter tea oil, sesame oil, safflower seed oil, corn oil, black rice oil, and walnut. Alien vegetable oils of various carbon chain lengths, such as oil or grape seed oil, provide the main source of organic nutrients needed for the growth of anaerobic microorganisms in the field to stimulate the number of native protozoa. The vegetable oil may be used in an amount of 46 to 55 parts by weight, such as 48, 50, 52 or 54, etc., but is not limited thereto.

Furthermore, the biodegradable surfactant may be selected from a lipophilic surfactant or a hydrophilic surfactant, wherein the lipophilic surfactant is preferably selected from lecithin; and the hydrophilic surfactant is preferably selected. It is not limited to butyl cellosolve (Simple Green, SG, also known as 2-butoxyethanol) or natural sapindus extract saponin. The biodegradable surfactant may be used in an amount of 3.7 to 5 parts by weight, such as 3.8, 4.0, 4.2, 4.4, 4.5, 4.6 or 4.8, etc., but is not limited thereto.

In addition, in an embodiment of the present invention, the sustained-release composite matrix may further comprise a vitamin, such as a vitamin B group, particularly a vitamin, depending on the soil quality of the soil in which the groundwater is contaminated or the microorganism species thereof. B12. The vitamin is used as a nutrient for the microorganisms in the field to promote its growth, and it is dissolved in the oil droplets or in liquid water. The vitamin may be used in an amount of 2.3 to 4 parts by weight, such as 2.4, 2.6, 2.8, 3.0, 3.2, 3.4, 3.6 or 3.8, etc., but is not limited thereto. In one embodiment, the vitamin comprises, for example, vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B12, vitamin B6, vitamin B2, vitamin B3, and vitamin B5.

In addition, the anatrophic microbial nutrient substrate of the present invention further adds 0.46-0.5 parts by weight of magnesium hydroxide (Mg(OH) ₂ ) based on the organic anaerobic substance, which can directly release the hydroxide ions and buffer. It is also possible to form a long-acting buffer substance by mixing magnesium hydroxide with a fatty acid in an organic anaerobic substance to form a fatty acid magnesium salt and glycerin. The magnesium hydroxide may be used in an amount of 0.46 to 0.5 parts by weight, such as 0.47, 0.48 or 0.49, etc., but is not limited thereto.

Furthermore, the anatrophic microbial nutrient substrate of the present invention contains 46 to 50 parts by weight of water, such as 47, 48 or 49, etc., but is not limited thereto. The water is mainly used to fully mix the above components, in particular, the biodegradable surfactant system and the vegetable oil are mixed in water to form an oil droplet suspension, wherein the vegetable oil and the biodegradable surfactant (and vitamin) form an oil. The drops (ie, oil droplets) are uniformly suspended and dispersed in the liquid water, and the fatty acid magnesium salt and glycerin formed by mixing the magnesium hydroxide with the vegetable oil (fatty acid) are also mixed in the oil droplet particles. In one embodiment, the oil droplet particles have a particle size ranging from 1-2 [mu]m (micrometers), such as 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9 [mu]m, but are not limited thereto.

In a first embodiment of the present invention, the present invention provides a method for producing a pH-reducing microbial nutrient matrix for buffering a water body, which comprises the following steps: First, in sequence Soybean oil 0.46g (g), magnesium hydroxide 0.046g, lecithin 0.037g, vitamin 0.023g and pure water 0.46ml (ie containing 46 parts by weight of vegetable oil, 4.6 parts by weight of magnesium hydroxide, 3.7) Parts by weight of biodegradable surfactant, 2.3 parts by weight of vitamins and 46 parts by weight of water), after preliminary mixing, stirring with a homogenizer at 25 ° C and 12,000 rpm for 15 minutes, so that the above ingredients are thoroughly ground and By uniformly mixing, an anaesthetic microbial nutrient substrate in the form of an oil droplet suspension is obtained, wherein the oil droplet particles in the oil droplet suspension have a particle size ranging from about 1 to 2 μm. The above vitamins include, for example, 0.26% by weight of vitamin A, 0.98% of vitamin D3, 0.05% of vitamin E, 0.01% of vitamin K3, 98.19% of vitamin B12, 0.06% of vitamin B6, and 0.35% of vitamin B2. 0.07% of vitamin B3 and 0.03% of vitamin B5.

In a manufacturing method according to a second embodiment of the present invention, 0.55 g (g) of soybean oil, 0.005 g of magnesium hydroxide, 0.05 g of lecithin, 0.04 g of vitamins and 0.5 mL of pure water are added to a container (ie, Containing 55 parts by weight of vegetable oil, 0.5 parts by weight of magnesium hydroxide, 5 parts by weight of biodegradable surfactant, 4 parts by weight of vitamins and 50 parts by weight of water), after preliminary mixing, using a homogenizer at 25 ° C and a rotation speed Stirring at 12,000 rpm for 15 minutes, the above ingredients are thoroughly ground and uniformly mixed to obtain an anaesthetic microbial nutrient substrate in the form of an oil droplet suspension, wherein the oil droplet particles in the oil droplet suspension have one The particle size ranges from about 1 to 2 μm. The composition of the vitamin is the same as in the previous embodiment.

In a manufacturing method according to a third embodiment of the present invention, a soybean oil is added with 0.5 g (g) of soybean oil, 0.0048 g of magnesium hydroxide, 0.04 g of lecithin, 0.03 g of vitamins, and 0.48 ml of pure water (that is, Containing 50 parts by weight of vegetable oil, 0.48 parts by weight of magnesium hydroxide, 4 parts by weight of biodegradable surfactant, 3 parts by weight of vitamins and 48 parts by weight of water), after preliminary mixing, using a homogenizer at 25 ° C and a rotation speed Stirring at 12,000 rpm for 15 minutes, the above ingredients are thoroughly ground and uniformly mixed to obtain an anaesthetic microbial nutrient substrate in the form of an oil droplet suspension, wherein the oil droplet particles in the oil droplet suspension have one The particle size ranges from about 1 to 2 μm. The composition of the vitamin is the same as in the first embodiment.

The buffered water body pH value of the anaerobic microbial nutrient matrix can be re-prepared before use in the current site to ensure that it retains the form of an oil-drop suspension, and the product can be preserved in a cool place. About 30 days. After the completion of the production and before the remediation of the site, Diluted with water by about 10 to 30 times (the actual dilution ratio depends on the permeability of the existing aquifer), and then injected into the groundwater aquifer through the groundwater well.

When the present invention was subjected to the following degradation experiments, the materials shown in Table 1 below were first prepared, wherein the aseptic control group contained TCE (1 mg/L), ground water (150 mL), soil (50 g), NaN ₃ (0.5 mg), HgCl _{2 .} (0.25 mg), the NaN ₃ and HgCl _{2 are} used as a bactericide; the existing anaerobic control group contains TCE (1 mg/L), ground water (150 mL), soil (50 g), and existing anaerobic substance (0.1 g). The existing anaerobic substance comprises about 1% by weight of sodium lactate; and the experimental group of the buffered anaerobic substance of the invention comprises TCE (1 mg/L), ground water (150 mL), soil (50 g), buffer anaerobic substance of the invention ( 0.1 g), the present invention is herein prepared using the buffered anaerobic formulation ratio of the above first embodiment (that is, comprising 46 parts by weight of vegetable oil, 0.46 parts by weight of magnesium hydroxide, and 3.7 parts by weight of biodegradable interface). Active agent, 2.3 parts by weight of vitamins and 46 parts by weight of water). In addition, the contents of the aseptic control group, the existing anaerobic control group and the buffered anaerobic test group of the present invention further comprise a reducing agent Na ₂ S and an anaerobic indicator resazurin.

When the existing anaerobic substance (control group, sodium lactate, sodium lactate) and the buffered anaerobic substance of the invention (experimental group, magnesium hydroxide added) were subjected to microbial batch degradation test, the chlorine-containing contaminant was selected to be trichloroethylene (TCE). Representative, control group and experimental assembled bottles were incubated in the dark chamber of the anaerobic chamber for 80 days, and the temperature was maintained at room temperature of 25 °C. The groundwater and soil system in this experiment was taken from the vinyl chloride plant of Taiwan's vinyl chloride company in Kaohsiung Linyuan Industrial Zone (No. 1 Industrial Road, Linyuan, Kaohsiung City), which is a chlorinated organic pollution site adjacent to the sea (sampling site) The site is about 150~200 meters away from the Gaopingxi Riverbed and about 2000 meters away from the Gaopingxi River estuary. The Dehalococcoides sp. is a native anaerobic bacterium in the existing aquifer soil, not added. It has a good anaerobic reductive dechlorination effect on chloroolefins, chloroalkanes, chlorophenols and chlorobenzenes, so it can be used as an indicator strain for anaerobic microorganisms degrading chlorinated organic pollutants. .

As shown in Table 2 below, it discloses the content of trichloroethylene and its degradation by-products measured during the 80-day culture period of the above-mentioned aseptic control group, the control group and the experimental group, wherein the control group of the existing anaerobic substance (including sodium lactate) is used. After the third day of culture, the two by-products of trichloroethylene (TCE) degradation were found to be 1,2 dichloroethylene (cDCE) and 1,1-dichloroethylene (1,1-DCE); After the fifth day, another by-product of vinyl chloride (VC) was found after degradation of trichloroethylene. After the 80th day of culture, the concentration of vinyl chloride was maintained at 0.09 mg/L, and the degradation efficiency of trichloroethylene was only 67%. On the other hand, the experimental group of the buffered anaerobic substance (containing magnesium hydroxide) of the present invention found that after the third day of culture, the three by-products of trichloroethylene (TCE) degradation were cis 1,2 dichloroethylene (cDCE), 1,1 dichloroethylene (1,1-DCE) and vinyl chloride (VC) appeared simultaneously; when the culture reached the 30th day, no vinyl chloride concentration was detected; after the 80th day of cultivation, trichloroethylene The degradation efficiency has reached 100%.

As shown in Table 3 below, it reveals the change trend of the pH value measured during the 80-day culture period of the above control group and the experimental group, wherein the pH value of the control group of the existing anaerobic substance (sodium lactate-containing) is cultured until the fifth day. It is weakly acidic (5.8) and continues to decrease with time; when cultured to the 80th day, the pH is directly reduced to acidity (4.5), indicating that acidification hydrolysis causes organic acid accumulation due to anaerobic degradation reaction. On the other hand, in the experimental group for buffering anaerobic substances (including magnesium hydroxide) of the present invention, the pH was gradually lowered due to the use of the substrate by microorganisms, but the pH remained approximately neutral until the 80th day of culture (6.9). ). Comparing the results of Tables 2 and 3 above, the control group of the existing anaerobic (sodium lactate) decreased the pH value, which was not conducive to the growth and reproduction of the reduced-halogen bacteria, which not only caused the degradation of trichloroethylene (TCE). The by-products of cis 1,2 dichloroethylene (cDCE), 1,1 dichloroethylene (1,1-DCE) and vinyl chloride (VC) are produced and accumulated without further degradation, and the overall degradation efficiency of trichloroethylene is also poor. . On the other hand, the experimental group for buffering anaerobic substances (including magnesium hydroxide) of the present invention is suitable for maintaining the pH value of groundwater and soil environment close to neutral, and is more suitable for long-term growth and reproduction of dehalogenating bacteria, and thus trichloroethylene ( The degradation by-products of TCE), cis 1,2 dichloroethylene (cDCE), 1,1 dichloroethylene (1,1-DCE) and vinyl chloride (VC), are not cumulatively excessive, and the overall degradation effect of trichloroethylene is also higher. good.

As shown in Table 4 below, it reveals the change trend of the organic carbon concentration measured during the 80-day culture period of the above control group and the experimental group, wherein the buffered anaerobic substance (hydrogen-containing) of the present invention is added at the same 1% anaerobic amount. Magnesium oxide) has a higher organic carbon concentration than the existing anaerobic materials (sodium lactate containing), and thus exhibits a longer-lasting effect on the anaerobic reaction using the buffered anaerobic substance of the present invention.

Table 4, control group and experimental group measured organic carbon concentration during 80 days of culture

As shown in Table 5 below, it discloses the measurement data of the above-mentioned aseptic control group, the control group and the experimental group, wherein the number of bacteria in the aseptic control group at 0 days and 80 days is 0; the existing anaerobic substance (containing sodium lactate) is added. In the control group, the growth rate of bacteria from 0 days to 80 days was about 296 times; and the growth rate of the number of bacteria from 0 days to 80 days in the experimental group to which the buffered anaerobic substance (containing magnesium hydroxide) of the present invention was added was about 6474 times, significantly increased to nearly 22 times the growth rate of the control group. From the results, it can be seen that the experimental group of the buffered anaerobic substance (containing magnesium hydroxide) of the present invention is advantageous for maintaining the pH value of the pH in the neutral state, and therefore the pH acid after the long-term degradation reaction by the anaerobic microorganism (represented by Dehalococcoides ) The amount of change in alkali value is still small, so it is suitable for the anaerobic microorganisms such as Dehalococcoides dehalogenating bacteria to grow and multiply stably. Therefore, the number of bacteria measured and the growth ratio are much higher than those of existing anaerobic substances (including sodium lactate).

Compared with the prior art, the anaerobic organic matrix with buffer function of the invention is additionally added with magnesium hydroxide (Mg(OH) ₂ ) based on organic anaerobic materials, so that hydroxide ions can be released for buffering, and at the same time, By mixing magnesium hydroxide with fatty acids in organic anaerobics to form fatty acid magnesium salts and glycerin, fatty acid magnesium salts and glycerin are further used as long-acting buffer substances for long time in remediation of groundwater environment (about 80 days) Maintaining the buffering capacity of the water body that is most suitable for the neutral pH environment of anaerobic microbial reactions, that is, neutralizing the anaerobic when the anaerobic microorganisms anaerobic reaction of the organic anaerobic acid to release the organic acid The organic acid produced by the reaction is used to maintain a neutral pH environment for a long time. Moreover, the buffered anaerobic organic matrix of the present invention requires only one infusion into the groundwater aquifer, which is sufficient to supplement the carbon source required for the cultivation of the existing anaerobic microorganisms for a long period of time and has a long-term buffering capacity.

Furthermore, the formulation of the present invention is a mixture of Mg(OH) ₂ added to a vegetable oil (such as soybean oil), and then a biodegradable surfactant (such as lecithin) is added separately, and after being added, the mixture is uniformly stirred by a homogenizer to become Finished product. The finished product has the form of an oil droplet suspension, that is, the vegetable oil and the biodegradable surfactant (and vitamins) form oil droplets uniformly dispersed and dispersed in the liquid water, and the magnesium hydroxide and the vegetable oil (fatty acid) are mixed to form the fatty acid magnesium. Salt and glycerin are also mixed in the oil droplets, and the product can be stored for up to about 30 days in a cool place. It can be diluted into the groundwater aquifer through the groundwater well after the completion of the production and before use, and then diluted with water by about 10 to 30 times (the actual dilution ratio depends on the permeability of the existing aquifer). Therefore, it is extremely simple in terms of process and use.

Therefore, as described above, in one embodiment, the present invention can provide a use of a water-reducing microbial nutrient matrix for buffering water pH, which can be used for the above-mentioned microbial nutrient substrate (46 to 55 parts by weight of vegetable oil, 0.46). ~0.5 parts by weight of magnesium hydroxide, 3.7 to 5 parts by weight of biodegradable surfactant, 2.3 to 4 parts by weight of vitamins and 46 to 50 parts by weight of water) pass through gravity flow, low pressure or high pressure perfusion A groundwater well of a chlorinated organic contaminated site is injected into a groundwater aquifer to cause local anaerobic microorganisms to degrade chlorinated organic pollutants in groundwater, wherein the anatrophic microbial nutrient matrix is long-acting with fatty acid magnesium salts and glycerol Buffer material to provide a long-term pH buffering capacity to effectively neutralize the organic acids produced by the reaction of anaerobic microorganisms (such as Dehalococcoides dehalogenating bacteria), and maintain the most suitable anaerobic microbial reaction in water for a long time. pH environment. The chlorinated organic contaminated site is preferably a chlorinated organic contaminated site adjacent to the sea, such as the Kaohsiung Linyuan Industrial Zone, especially the Taiwan Vinyl Chloride Company's vinyl chloride plant in Kaohsiung Linyuan Industrial Zone (Kaohsiung City Forest Park Industry) No.1), which is a chlorinated organic pollution site adjacent to the sea (the sampling site is about 150~200 meters away from Gaopingxi Riverbed and about 2000 meters away from Gaopingxihekou). The above-mentioned Taiwan vinyl chloride plant Linyuan vinyl chloride plant mainly uses ethylene and ethylene dichloride (EDC) to synthesize vinyl chloride (VC), when vinyl chloride or dichloroethane is discharged through factory wastewater or When the accidental leakage of the storage tank or pipeline exists in the wastewater, soil or groundwater of the factory, it is easy to increase the depth and extent of the soil pollution, so it must be rectified. The Linyuan vinyl chloride plant has been in operation for 22 years since its operation in 1992. Therefore, the original anaerobic microorganisms in the groundwater of the site are exposed to vinyl chloride (and/or dichloroethane) in the environment for a long time. That is to say, it has better degradation efficiency of vinyl chloride or dichloroethane, and because of its proximity to the estuary, the native anaerobic microorganisms in the area are also more adaptable to the environmental factors of the adjacent estuary soil (such as seasonal temperature change, salinity, soil composition). , chlorinated organic pollutant concentrates, groundwater volume and depth, other organic/inorganic pollutants, etc.); if the anaerobic organic matrix of the present invention is further injected into its groundwater aquifer via a groundwater well, it will promote local anaerobic Microbial degradation of chlorinated organic contaminants in groundwater and use of fatty acid magnesium salts and glycerol as long-acting buffers to provide a long-term pH buffering capacity for effective neutralization of anaerobic microorganisms (eg Dehalococcoides dehalogenating bacteria) The organic acid produced by the reaction, which is the most suitable environment for the anaerobic microbial reaction in the groundwater body which maintains the contaminated site of the chlorine-containing organic substance for a long time.

It should be noted that the "%" mentioned above in the present invention refers to "% by weight (wt%)" unless otherwise specified; the range of values (such as 10% to 11% of A) is included unless otherwise specified. The lower limit value (ie 10% ≦A ≦ 11%); if the value range does not define the lower limit value (such as B below 0.2%, or B below 0.2%), it means that the lower limit value may be 0 ( That is, 0% ≦ B ≦ 0.2%); the proportional relationship of the "weight parts" of each component can also be changed to the "weight percentage" proportional relationship after conversion.

The present invention has been disclosed in its preferred embodiments, and is not intended to limit the invention, and the present invention may be modified and modified without departing from the spirit and scope of the invention. The scope of protection is subject to the definition of the scope of the patent application.

Claims (12)

An anaerobic microbial nutrient substrate for buffering water pH value, comprising: 46-55 parts by weight of vegetable oil, 0.46-0.5 parts by weight of magnesium hydroxide, 3.7-5 parts by weight of biodegradable surfactant, 2.3~4 a part by weight of vitamins and 46 to 50 parts by weight of water; in the anatrophic microbial nutrient matrix, magnesium hydroxide is mixed with fatty acids of vegetable oil to form fatty acid magnesium salt and glycerin, and the anatrophic microbial nutrient substrate is made of fatty acid magnesium salt and glycerin It is a long-acting buffer material to provide pH buffering capacity. The anaerobic microbial nutrient substrate of the buffered water pH value according to claim 1, wherein the anatrophic microbial nutrient substrate comprises 46 parts by weight of vegetable oil, 0.46 parts by weight of magnesium hydroxide, and 3.7 parts by weight of the living organism. The surfactant was decomposed, 2.3 parts by weight of vitamins and 46 parts by weight of water. The anaerobic microbial nutrient substrate of the buffered water pH value according to claim 1, wherein the anatrophic microbial nutrient substrate has the form of an oil droplet suspension in which a plurality of oil droplets are suspended. particle. The anaerobic microbial nutrient substrate of the buffered water pH value according to claim 3, wherein the fatty acid magnesium salt and glycerin are mixed in a plurality of oil droplets of the oil droplet suspension. The anaerobic microbial nutrient substrate of the buffered water pH value according to claim 3 or 4, wherein the oil droplet particles have a particle size ranging from 1 to 2 micrometers. The anaesthetic microbial nutrient matrix of the buffered water pH value according to claim 1, wherein the vitamin is a vitamin B group. The absorbing microbial nutrient substrate of the buffered water pH value according to claim 6, wherein the vitamin B group comprises vitamin B12. As the application of the patent scope of the first item of the buffer water pH value of the ill-health A nutrient substrate, wherein the biodegradable surfactant is selected from the group consisting of lipophilic surfactants or hydrophilic surfactants. The buffered water pH-valued anaesthetic microbial nutrient substrate according to claim 8, wherein the lipophilic surfactant is selected from the group consisting of lecithin; the hydrophilic surfactant is selected from the group consisting of butyl cellosolve or sapindus extraction. Saponin. An anaerobic microbial nutrient matrix for buffering water body pH, comprising vegetable oil, magnesium hydroxide, biodegradable surfactant, vitamins and water, wherein magnesium hydroxide accounts for 0.46-0.5 of the total weight of the nutrient substrate of the anatrophic microorganism Gt%; in the anatrophic microbial nutrient matrix, magnesium hydroxide is mixed with fatty acids of vegetable oil to form fatty acid magnesium salt and glycerin, and the anatrophic microbial nutrient substrate uses fatty acid magnesium salt and glycerin as long-acting buffer substances to provide pH buffer ability. The use of a water-reducing microbial nutrient matrix for absorbing water pH value, which is to inject a anaerobic microbial nutrient substrate according to claim 1 or 10 into a groundwater aquifer through a groundwater well to promote the local anatomy Oxygen microorganisms degrade chlorinated organic pollutants in groundwater, wherein the anatrophic microbial nutrient matrix uses fatty acid magnesium salts and glycerin as long-acting buffer substances to provide pH buffering capacity to neutralize organic acids produced by anaerobic microbial reactions, And maintain a neutral pH environment suitable for anaerobic microbial reactions in water. The use of the buffered water pH value of the anaerobic microbial nutrient substrate according to claim 11, wherein the chlorine-containing organic pollutant is trichloroethylene.