TW201620463A - Manufacturing method of mineralized scaffold and device therefor - Google Patents

Abstract

A manufacturing method of mineralized scaffold and a device therefor are disclosed. The disclosed mineralized scaffold manufacturing device includes a bioscaffold having a pre-determined shape, an imaginary baseline extending from one end of the bioscaffold to the other thereof, a chamber having a space accommodating the bioscaffold and formed a corresponding sub-chamber space at two sides of the bioscaffold along the baseline. The disclosed is characterized by having the baseline as an axis, and the radial inner diameter of each sub-chamber space is gradually increased or decreased in sequence.

Description

Method and device for manufacturing mineralized stent

The present invention relates to tissue engineering, and more particularly to a method of manufacturing a mineralized stent and apparatus therefor.

According to the tissue engineer, the cells are implanted in a biomimetic scaffold, so that the cells grow into new tissues and organs on the bioscaffold, and the repairers for the human defect tissue are often used in the prior art. As a material for biological scaffolds, there are natural collagen, chitosan, hyaluronic acid, alginate, gelatin, etc., as well as artificial polylactic acid, polyglycolic acid, polylactic acid-glycolic acid, polycaprolactone. And a variety of different polymer materials.

Among them, the artificial polymer material is not as good as the natural polymer material in cell identification, but compared with the limited source and poor mechanical strength of the natural polymer material, the artificial polymer material can be It is mass-produced through synthesis and can control its size and structural strength. In contrast, artificial polymer materials are more suitable for use in the industrial field of tissue engineering.

The fact that artificial polymer materials are inferior to natural polymer materials in cell identification, such as polylactic acid-glycolic acid, is a good biological scaffold material, but its surface is hydrophobic and the affinity of cells is poor. As a result of its limitations in industrial applications, and in order to improve the lack of it, in the conventional technology, the surface of the bio-scaffold is modified to form a mineralized layer. Improve its biocompatibility and fineness Affinity of cells, it is pointed out in the prior art that the formation of bone-like apatite layers on biological scaffolds is a prerequisite for direct bone formation on biological scaffolds (Kokubo T.Novel biomedical materials based on glasses[J] .Materials Science Forum. 1999, 293: 65-82.).

Conventionally, the specific technical content for forming a mineralized layer on the surface of a biological stent is basically to place the biological stent to be mineralized into a supersaturated solution of a specific composition, so that the components in the solution are nucleated and grown on the stent. The surface, thus forming a mineralized layer on the surface of the biological scaffold, is a conventional technique that is solidified to form a mineralized layer on the surface of the bioscaffold, but because of its lack of control over the formation of the mineralized layer, therefore, The technology obtained after the implementation is only a single type of crystallized and dense mineralized layer, although the purpose of modifying the surface of the biological scaffold material is still achieved, but the bioscaffold is not increased in the field of tissue engineering industry. Application.

Accordingly, the main object of the present invention is to provide a method and apparatus for manufacturing a mineralized stent, the main technical feature of which is to form an environment having a concentration gradient by changing a space in a chamber of a device for manufacturing a biological stent. According to the formation of a mineralized layer of different density and diffusion range on the surface of the biological scaffold, the surface of the biological scaffold made by the various parts of the bio-scaffold has an unequal amount of deposited minerals, making it more suitable for tissue engineering. use.

In order to achieve the above object, the apparatus for manufacturing a mineralized stent provided by the present invention comprises a biological stent having a predetermined shape: an imaginary reference line extending from one end of the biological stent to the other end; a chamber a space for accommodating the biological support, and respectively forming a corresponding sub-chamber space on both sides of the biological support along the reference line; and the feature is that the reference line is an axis, and each of the sub-chamber spaces The radial inner diameter is gradually increasing or decreasing in sequence.

Specifically, the reference line is the axis, and the inner diameter of each of the sub-chamber spaces is changed in the same direction, one is gradually decreasing, and the other is increasing.

In order to facilitate changing the inner diameter of each of the sub-chamber spaces, the manufacturing device of the mineralized stent further comprises a hollow body, which is formed by a hollow inner space, and has a hollow body. The two ends are respectively disposed at two ends of the body, and the two inlet passages are respectively disposed on the respective ends, and each communicates with the hollow interior of the body portion, and is formed at one end of the corresponding end portion. Inflow port; a carrier member, in the body, between each of the ends for carrying the biological support.

Wherein, the carrier is in the shape of a plate, is accommodated in the body, and is bridged on the opposite chamber wall on the inner side of the body.

Wherein, the carrier further comprises a plate-shaped carrier, and the two pivoting systems are disposed coaxially on the two sides of the carrier and pivotally connected to the corresponding chamber wall of the body, so that the carrier can be The shaft rotates in the interior space of the body.

Wherein, the biological scaffold is in the form of a sheet and is disposed on the carrier.

Wherein, the carrier further comprises a magnet or a magnet, disposed on the carrier away from one end of the rotating shaft.

Each of the end portions is hollow, and each of the sub-chamber spaces is formed by a hollow inner space.

In addition, in order to make the manufacturing device of the mineralized stent provided by the present invention more flexible in industrial application, the specific structure includes a hollow body having a hollow body with a predetermined shape and two ends. The system is disposed at two ends of the hollow body, and the two inlet channels are respectively disposed in each end portion and communicate with the hollow interior of the body portion, and are individually at one end of the corresponding end portion Forming an inflow port; a chamber portion formed by a space inside the hollow interior of the main body; a carrier member disposed in the chamber portion and having a carrier interposed between the end portions The body is pivotally connected to the inner wall of the body at both ends, so that the carrier can be rotated as a shaft and rotate in the space of the chamber portion.

The slanting state of the carrier in the chamber portion is adjusted so that the carrier is horizontal, and the inner diameters of the sub-chamber spaces on both sides of the carrier are respectively single.

The carrier further includes two pivots which are coaxially disposed on both ends of the carrier and are pivotally connected to the corresponding chamber walls on the inner side of the body.

Wherein, the two ends of the body are emptied and are respectively closed by the ends.

Wherein, the carrier further comprises a magnet or a magnet, disposed on the carrier away from one end of the rotating shaft.

In addition, the manufacturing device of the mineralized stent is applied to a method for manufacturing a mineralized stent, and the manufacturing method specifically includes housing the biological stent in a closed chamber space and is used to form a mine. a solution of the layer is injected into the chamber space, so that the inorganic substance can be nucleated to form a mineralized layer on the surface of the biological scaffold; and the feature is that the chamber space is located on the organism based on the biological scaffold The inner diameters of the partial spaces on both sides of the stent are gradually increasing or decreasing in sequence.

Wherein, the inner diameter of each of the chamber spaces on the two sides of the biological support is opposite to each other.

(10) (10') ‧ ‧ Manufacture of mineralized stents

(20) ‧‧‧ Subject

(21)‧‧‧ Body

(22) (22’) ‧ ‧ end

(221’) ‧‧‧shell

(222’) ‧ ‧ openings

(23) ‧‧‧ Inflow

(231)‧‧‧ Inlet

(30) (30') ‧ ‧ Chambers

(31) (32) ‧ ‧ sub-room space

(40) (40’) ‧‧‧Send

(41) ‧ ‧ carrier

(42) ‧‧‧ pivot

(43)‧‧‧Magnetic magnets

(44) ‧‧‧Fixed tablets

(45) (45') ‧ ‧ shims

(46) (46’) ‧ ‧ perforation

(50) (50’) ‧ ‧ biological stent

(d) ‧ ‧ baseline

The first figure is an exploded perspective view of a preferred embodiment of the present invention.

The second drawing is a combined perspective view of a preferred embodiment of the present invention.

Figure 3 is a cross-sectional view of a preferred embodiment of the present invention taken along the line a-a of Figure 2;

The fourth figure is a simplified schematic diagram of a third embodiment of a preferred embodiment of the present invention.

Figure 5 is a cross-sectional view of a preferred embodiment of the present invention taken along the line a-a of Figure 2;

Figure 6 is a simplified schematic view of a fifth embodiment of a preferred embodiment of the present invention.

Figure 7 is an exploded perspective view of another preferred embodiment of the present invention.

Figure 7 is a perspective view of a combination of another preferred embodiment of the present invention.

Figure 9 is a cross-sectional view of another preferred embodiment of the present invention taken along the line sec of Figure 9-9.

First, referring to the first to sixth figures, in the manufacturing apparatus (10) of the mineralized stent provided in a preferred embodiment of the present invention, the main body comprises a main body (20) and a cavity. The chamber portion (30), a carrier member (40), and a plurality of biological stents (50).

The main body (20) is hollow and has a body portion (21), which is a hollow shell with two ends permeable, and the two end portions (22) are respectively disposed on the openings at both ends of the body portion (21). The two ends of the body (21) are closed, and the two inlet channels (23) are respectively disposed on the end portions (22) to communicate with the hollow inner space of the body portion (21). And each of them forms an inlet port (231) at one end of the corresponding end portion (22).

The chamber portion (30) is an accommodation space formed by a space inside the hollow portion of the main body (20).

The carrier member (40) is substantially plate-shaped and is received in the chamber portion (30), and is pivotally connected to the inner wall of the body portion (21) on both sides of the body portion (21), and is interposed therebetween. Between the ends (22), 俾 The carrier (40) is rotated in the space of the chamber portion (30) by the shaft pivotally connected to the body portion (21).

Each of the biological scaffolds (50) is a film sheet body made of a synthetic polymer material, which is respectively disposed on the carrier member (40), and is rotatable with the carrier member (40) in the chamber. The position of the part (30) is changed.

Accordingly, the manufacturing device (10) of the mineralized stent is applied to a method for manufacturing a mineralized stent, that is, by a method of mineralization, a two-phase different composition solution is respectively passed through each end portion ( 22) The upper inflow port (231) is injected into the chamber portion (30), and the mineralization of the nucleation growth is performed on the surface of each of the biological scaffolds (50), thereby achieving the biological scaffold ( 50) The purpose and function of surface modification.

At the same time, in order to achieve the purpose and effect of the degree of mineralization of each part of the surface of each biological scaffold (50), the plane in which each bioscaffold (50) is located is an imaginary reference line (d). The chamber portion (30) forms a corresponding sub-chamber space (31) (32) on each side of each of the biological supports (50) along the reference line (d), and the reference line (d) is used as an axis. The radial inner diameters of the sub-chamber spaces (31) (32) are sequentially decreased or increased, respectively, so as to be formed by the chamber portions (30) on both sides of each part of the biological stent (50). Different volumes of space provide different amounts of solutes in different parts of the biological scaffold (50), so as to achieve the purpose and effect of different mineralization levels of each part of the bioscaffold (50).

In order to change the inner diameter of each of the sub-chamber spaces (31) (32), the carrier (40) can be rotated in the chamber portion (30) to deflect the reference line, thereby achieving The inner diameter of the sub-chamber space (31) (32) is decreasing, and the other is increasing in increments.

Specifically, the carrier (40) has a hollow plate-shaped carrier (41). The system is in the chamber portion (30), and the two pivots (42) are respectively coaxially disposed on the opposite sides of the carrier (41), and the inner chamber of the body (21) The wall is pivotally connected to form the rotating shaft, so that the carrier (41) can be rotated as an axis.

To enable the carrier (40) located inside the closed chamber portion (30) to be controlled to rotate, a suitable magnet or sense can be placed on the carrier (40) away from one end of the shaft. a magnet (43), which is external to the main body (20), acts on the magnetic force between the external magnet or an external magnet (not shown) and the magnet or the magnet (43), so that the load The piece (40) is turned on.

In order to properly bond each of the biological stents (50) in the form of a film sheet to the carrier member (40), in the embodiment, the carrier member (40) further comprises a pair of parallel fixings. The sheet (44) is inserted into the carrier (40), and a pair of spacers (45) parallel to each other are sandwiched between the fixing pieces (44), and the plurality of perforations (46) are respectively Through the respective fixing pieces (44) and each of the spacers (45), the apertures are smaller than the outer diameters of the respective biological brackets (50), so that the biological brackets (50) are clamped to the respective spacers ( 45) is exposed through the corresponding perforation (46) to be in contact with the space of the chamber portion (30).

In the manufacturing device (10) of the mineralized stent provided in the above embodiment, the carrier (40) is rotatable to carry each of the biological stents (50). The specific configuration is to control the rotation angle of the carrier (40) to adjust the variation of the inner diameter of each of the sub-chamber spaces (31) (32), so as to meet the actual industrial needs, therefore, if the industry When there is a specific need, the manufacturing device (10) of the mineralized stent can also be used in a state in which the inner diameters of the sub-chamber spaces (31) and (32) are respectively in a single state. The figures shown in the fifth and sixth figures.

In addition, the specific technical content for changing the inner diameter of the space on both sides of the biological support is not limited to the specific disclosure of the foregoing embodiment, and it is also another preferred embodiment of the present invention as shown in the seventh to ninth embodiments. In the same manner as the manufacturing device (10') of the mineralized stent disclosed in the embodiment, the member is more compact.

Specifically, the manufacturing device (10') of the mineralized stent disclosed in the embodiment has a two-end portion (22'), a chamber portion (30'), a carrier member (40'), and In most biological stents (50'), each of the ends (22') has a hollow wedge-shaped casing (221'), and an opening (222') is opened in the casing (221'). One side and the other side facing each other coaxially.

The carrier (40') is sandwiched between the ends (22') and has two spacers (45') parallel to each other, and a plurality of perforations (46') are respectively disposed on the spacers. (45'), the inner diameter is slightly smaller than the outer diameter of each of the biological stents (50'), and is in communication with each of the openings (222').

Each of the bio-scaffolds (50') is a film sheet-like body, each of which is sandwiched between each of the spacers (45'), and is exposed to each of the openings (222') via a corresponding perforation (46'). Within the space.

The chamber portion (30') is substantially formed by an inner space of each of the housings (221') and each of the perforations (46') communicating with each other via the openings (222'), and by a triangular inner space of the casing (221'), and a sub-chamber space (31') (32') having a different inner diameter is formed on both sides of each of the biological supports (50'), thereby achieving the borrowing from the foregoing embodiment. The same effect is achieved by rotating the carrier to change the inner diameter of the sub-chamber space.

In other words, the manufacturing device (10') of the mineralized stent is applied to the mineralization of the stent, and the plane in which the biological stent (50') is located is used as a reference line, and the reference line is a shaft, the radial direction of each of the sub-chamber spaces (31') (32') located on either side of each of the biological supports (50') The inner diameter, one is incrementally changed, and the other is a decreasing one, so that the formed mineralized layer has different deposition states at different locations as in the previous embodiment.

(10) ‧ ‧ manufacturing device for mineralized stent

(20) ‧‧‧ Subject

(21)‧‧‧ Body

(22) ‧ ‧ end

(23) ‧‧‧ Inflow

(231)‧‧‧ Inlet

(40) ‧‧‧Ship

(41) ‧ ‧ carrier

(42) ‧‧‧ pivot

(43)‧‧‧Magnetic magnets

(44) ‧‧‧Fixed tablets

(45) ‧‧‧shims

(46) ‧‧‧Perforation

(50) ‧ ‧ biological stent

Claims (14)

A manufacturing device for mineralized stents, comprising: a biological stent of a predetermined shape: an imaginary reference line extending from one end of the biological stent to the other end; a chamber portion accommodating the biological stent in a space, and A corresponding sub-chamber space is formed on each side of the biological support along the reference line; and the radial inner diameter of each of the sub-chamber spaces is gradually increased or decreased in sequence according to the reference line. According to the manufacturing apparatus of the mineralized stent according to the first aspect of the invention, wherein the reference line is the axis, the inner diameter of each of the sub-chamber spaces changes in the same direction, one is gradually decreasing, and the other is gradually decreasing. increase. The manufacturing device for mineralized stent according to claim 1 or 2, further comprising: a hollow body formed by a hollow inner space having a hollow body and two ends The system is disposed at two ends of the body, and the two inlet channels are respectively disposed on each end portion, and each of them communicates with the hollow interior of the body portion, and an inflow port is formed at one end of the corresponding end portion; A carrier member is disposed in the body between the ends to carry the biological support. The apparatus for manufacturing a mineralized stent according to claim 3, wherein the carrier is in the form of a plate, is received in the body, and is bridged to the opposing chamber wall on the inner side of the body. The apparatus for manufacturing a mineralized stent according to claim 4, wherein the carrier further comprises a plate-shaped carrier, and the two pivotal systems are coaxially disposed on opposite sides of the carrier, and are pivotally connected. On the corresponding chamber wall of the body, the carrier is rotated as a shaft to be rotated in the interior space of the body. The apparatus for manufacturing a mineralized stent according to claim 5, wherein the biological stent is in the form of a sheet and is disposed on the carrier. The apparatus for manufacturing a mineralized stent according to the fourth aspect of the invention, wherein the carrier further comprises a magnet or a magnet, disposed on the carrier away from one end of the rotating shaft. The apparatus for manufacturing a mineralized stent according to claim 3, wherein each of the end portions is hollow, and each of the sub-chamber spaces is formed by a hollow internal space. A manufacturing device for a mineralized stent comprises: a hollow body having a hollow body of a predetermined shape, two ends, which are respectively disposed at two ends of the hollow body, and two flow paths are respectively arranged in Each of the end portions is in communication with the hollow interior of the body portion, and an inlet port is formed at one end of the corresponding end portion; a chamber portion is formed by a space inside the hollow portion of the body; a carrier member Provided in the chamber portion, having a carrier interposed between the end portions and being in the body portion, and pivoting the two ends to the corresponding inner wall of the body portion, thereby enabling the carrier to It rotates in the space of the chamber portion as a rotating shaft. The apparatus for manufacturing a mineralized stent according to claim 9, wherein the carrier further comprises two pivots disposed coaxially with each other on both ends of the carrier, and the inner side of the body Corresponding to the wall of the chamber. The apparatus for manufacturing a mineralized stent according to claim 10, wherein both ends of the body are permeable and are respectively closed by the ends. A manufacturing device for mineralized stent according to claim 9 of the patent application, wherein the carrier The system further includes a magnet or a magnet, disposed on the carrier away from one end of the rotating shaft. A method for manufacturing a mineralized stent, wherein a biological stent is accommodated in a closed chamber space, and a solution for forming a mineralized layer is injected into the chamber space, so that inorganic substances can be allowed on the surface of the biological stent. The nucleation grows into a mineralized layer; the characteristic is that the inner diameter of the space of the chamber on both sides of the biological support is gradually increasing or decreasing in sequence according to the biological support. The method for manufacturing a mineralized stent according to claim 13 , wherein the inner diameter of each of the chamber spaces on the two sides of the biological stent is opposite to each other.