TW201520334A - Cell culture system - Google Patents

Abstract

The present invention provides a cell culture system, which comprises a carrier for carrying cells, and a light-emitting device for connecting the carrier, wherein the light-emitting device includes a wavelength adjustable light source that used by NBI (Narrow Band Imaging).

Description

Cell culture system

The disclosure relates to a cell culture system.

Cell culture technology is the core technology of the biotechnology industry. It uses artificial culture methods to carry out large-scale culture of cells outside the organism, thereby rapidly producing various biotechnology products and applying them to raw materials, medicines, vaccines, and biological pesticides. And other industries. The purpose of cell culture is to infer culture in vitro to facilitate obtaining a large number of target cells in a short period of time, and to observe the characteristics of various tissues in the living body by observing changes in cells during the culture. Furthermore, by adding different compounds to the cell culture process, it is also possible to investigate the effects of the compound on the cells, including whether cytotoxicity occurs or substances that promote cell growth.

Therefore, Sui Dynasty has developed a continuous cell research platform to facilitate the development of related fields, that is, to develop a cell culture system that can instantly monitor the growth of cells to be tested while ensuring constant conditions in the culture environment. To determine cell growth, differentiation, apoptosis and tight junctions, and as a result of drug stimulation.

The cell is the most basic unit of biology, and it is also the most basic observation target of biomedical science research. Because the growth and development of cells is extremely susceptible to the surrounding environment It is necessary to cultivate the required cells in a strictly controlled environment to ensure that the cells are based on research objectives and produce data with reference value. In the current research cell operation, the incubator is basically equipped to maintain the temperature, humidity and air content in a specific space, so that the culture can be preserved or grown in a constant environment. Furthermore, during the research, the growth conditions are often changed during the cell culture process, and the results are observed to verify some scientific hypotheses. Therefore, the equipment requirements in the field of biomedical research are able to observe without interference. Morphological changes in cells in culture.

However, in the conventional device for culturing cells, the microscope is placed in the incubator to monitor the cell growth in an instant, but since the microscope needs to be placed in the incubator, it cannot be flexibly applied to the existing cell culture program.

Moreover, the culture dish is placed in the incubator, and when the culture dish is removed from the incubator, the cells are exposed to environmental changes, causing the cells to mutate, so that the device for culturing the cells is not movable.

In addition, the incubator cannot regulate cell growth or inhibition, and initiate drug or chemical activity.

Therefore, how to design a cell incubator that meets the needs has become a problem that is currently being pursued.

The present disclosure provides a cell culture system comprising a carrier for carrying cells, and a light-emitting device having an adjustable wavelength light source connected to the carrier, the light source being a light source for narrow-band image (NBI).

Further, the cell culture system according to the present disclosure will be described in detail later with reference to the examples and the drawings.

1,1'‧‧‧ cell culture system

10‧‧‧ Vehicles

10a‧‧‧ Carrier

10b‧‧‧temperature structure

10c‧‧‧Base

100‧‧‧Electrical heating elements

100a‧‧‧heating rod

100b‧‧‧thermal clip

101‧‧‧Cover parts

101a‧‧‧ body

101b‧‧‧ cover

102‧‧‧ Magnetic components

11‧‧‧Lighting device

110‧‧‧Light source

110a‧‧‧Red light

110b‧‧‧Blue

12‧‧‧temperature control device

13‧‧‧ gas control device

14‧‧‧Liquid control device

140‧‧‧Control valve

15‧‧‧

16‧‧‧ body

8‧‧‧Microscope

9‧‧‧ Petri dishes

T‧‧‧ channel

1 is a schematic perspective view of a cell culture system according to an embodiment of the present invention; FIG. 2 is a perspective exploded view of the carrier of FIG. 1; FIG. 3 is another embodiment of the cell culture system of the present disclosure. 3 is a schematic perspective view of a liquid control device of FIG. 3; and FIG. 5 is a perspective view of another embodiment of the cell culture system of the present disclosure.

The embodiments of the present disclosure are described below by way of specific embodiments, and those skilled in the art can readily appreciate other features of the disclosure.

It is to be understood that the structure, the proportions, the size and the like of the present invention are only used to clarify the disclosure of the specification for the understanding and reading of those skilled in the art, and are not intended to limit the disclosure. The conditions are limited, so it is not technically meaningful. Any modification of the structure, change of the proportional relationship or adjustment of the size should remain in this book without affecting the effectiveness and the purpose of the disclosure. It is disclosed that the disclosed technical content can be covered. In the meantime, the terms "upper", "bottom", "inside", "outside" and "one" are used in this description for convenience of description only and are not intended to limit the disclosure. The scope, the change or adjustment of its relative relationship, without the substance of the substantive change, It is also considered to be the scope of implementation of this disclosure.

As shown in FIG. 1, the cell culture system 1 of the present disclosure comprises: a carrier 10 for carrying cells, and a light-emitting device 11 connected to the carrier 10.

The light-emitting device 11 has an adjustable wavelength light source 110, and the light source 110 includes red light 110a, blue light 110b, and green light (not shown). Since the light source 110 can be tuned to a wavelength, the cells can be observed, adjusted for growth or inhibition, and tested for drug reactions. For example, three primary colors of light (RGB) (ie, red, green, and blue) are mutually blended into white light at different ratios for observation.

In the present embodiment, the light source 110 is used for immediate observation of cells, for example, cell morphology observation by visible light, that is, observation of cell growth, differentiation, apoptosis change, and tight junction state. Alternatively, the light source 110 can be an excitation source of a fluorescent dye. The light source 110 can also emphasize the image function and instantly transmit the image to a display screen such as a computer for the user to observe, for example, performing Narrow Band Imaging (NBI) imaging to make the cell image more clear.

The NBI uses a specific double narrow wavelength optical principle to improve imaging quality. In detail, the longer the wavelength of a general light, the stronger the penetration force to the tissue, but it is easy to scatter, and the shorter the wavelength of the light, the poor penetration of the tissue, but it is not easy to scatter, thereby improving the resolution and the degree of resolution.

Furthermore, when the light source 110 is used to regulate cell growth or inhibition, light of different wavelengths has a different effect, for example, cells are proliferated by red light 110a, and cell growth is inhibited by blue light 110b.

Moreover, the light source 110 is used for a drug (such as a light-sensitive drug, light induced The test of the reaction is to test the response of the cells to the drug by different wavelengths. Specifically, at different wavelengths of light, a drug or chemical can initiate its activity via illumination, causing it to react to cells (toxic to cells, or increase cellular activity), thereby testing the drug.

The carrier 10 is used for cell maintenance. If the heat preservation effect is not good, the cell growth is poor, so the carrier 10 can be made of a high heat capacity material, so that the heat preservation effect is good, for example: iron ( 3.537 Cv), aluminum (3.45 Cv), where unit Cv is J. Cm ^-3 . K ^-1 .

In this embodiment, the carrier 10 includes a carrier 10a for carrying cells, a temperature regulating structure 10b for thermally conducting the carrier 10a, and a base 10c, so that the carrier 10 constitutes a container, and The cell line is placed in a Petri dish 9, and the Petri dish 9 is placed on the carrier 10a so that the Petri dish 9 is placed in the carrier 10.

Furthermore, as shown in FIGS. 1 and 2, the constant temperature structure 10b includes an electrothermal heating element 100, and a covering member 101 surrounding the side of the carrier 10a and the side of the electrothermal heating element 100, and The electrothermal heating element 100 contacts the carrier 10a, and the covering member 101 has a passage t between the supporting member 10a, so that the covering member 101 serves as a water jacket type heating member.

The electric heating method directly heats the bottom of the culture dish 9 and conducts it by heat conduction, so that the temperature changes rapidly, and thus it is suitable for an environment with large temperature variability. Therefore, the bottom of the carrier 10a and the base 10c are made of an electric heating material or a heat conducting material to directly conduct heat to the base 10c. Therefore, the electrothermal heating element 100 can include a heating rod 100a located in the channel t, or a heat conducting clip clamping the carrier 10a. 100b.

The water jacketed is indirectly heated by a fluid to perform radiation or convection, so that it can maintain a stable ambient temperature for a long time (approximately 4 to 5 times the time of the electrothermal type), and simultaneously The culture solution in the covering member 101 is heated. Therefore, the covering member 101 can include a sleeve body 101a for receiving the carrier member 10a and the heat conducting clip 100b, and a cover body 101b for covering the sleeve body 101a.

As can be seen from the above, the carrier 10 of the present disclosure achieves a good constant temperature environment by electrothermal heating (maintaining the temperature of the culture dish 9) and water jacket heating (maintaining the ambient temperature) to achieve cell tissue maintenance. The purpose.

Moreover, the carrier 10 has a magnetic component 102, i.e., it contains a magnet, such as a permanent magnet or an electromagnet, to quickly complete assembly of the carrier 10.

In addition, the material forming the carrier 10 may be a high light transmissive material, for example, electrothermal glass or heat conductive glass, etc., in order to facilitate observation of the cells of the culture dish 9, and the image observation effect is better.

As shown in FIG. 3, the cell culture system 1 of the present disclosure includes a temperature control device 12, a gas control device 13, and a liquid control device 14.

The temperature control device 12 mechanically integrates the carrier 10 to regulate the temperature regulating structure 10b and control the temperature of the carrier 10.

The gas control device 13 communicates with the carrier 10 in a gas-like manner to control the gas composition of the culture vessel 9. In the present embodiment, the gas control device 13 is an inflatable gas cylinder. In the laboratory, the gas control device 13 can use indoor gas as a gas required for cell culture to supply to the carrier 10 and simultaneously replenish the gas; if not in a laboratory (such as outdoors), Gas in the gas control device 13 can be supplied to the cells. Therefore, the gas control device 13 can use the cell culture system 1 and maintain the environmental humidity of the culture dish 9 by the design of the gas cylinder when the desired gas cannot be obtained in the environment.

The liquid control device 14 is connected to the carrier 10 in a flow-through manner, thereby supplying or replacing a liquid such as a culture liquid to the carrier 10 to maintain a culture environment, such as extracting a part of waste liquid, injecting a part of new liquid or medicine, Chemicals.

In the present embodiment, as shown in FIG. 4, the liquid control device 14 utilizes an automatic control mode, such as using the control valve 140 and a timer (not shown), to periodically change the culture fluid, such as the direction of the arrow, to avoid damage. The environment of the culture dish 9 and the liquid control device 14 can be driven by a pump 15 to form a circulating inertia, thereby mimicking circulation in the human body to achieve a biomimetic effect.

In addition, the cell culture system 1 of the present disclosure includes a power source (not shown). In the laboratory, the indoor power source can be used as the system power, and the battery is charged at the same time, while in the non-laboratory, the battery is used to supply power. Therefore, the cell culture system 1 is required for maintenance operation, and a battery can be assembled inside it for use in a non-powered environment.

Therefore, with the above design, the power supply, the gas control device 13 and the liquid control device 14 of the present disclosure can be portablely equipped to facilitate cell culture.

As shown in FIG. 5, the cell culture system 1' of the present disclosure can be modularized, and the plurality of carriers 10 arranged by the array 16 are arranged for the microscope 8 to observe, for example, for drug poisoning. Analysis and analysis. In the case of drug toxicology detection and analysis, different drug concentration tests are performed to compare the control group with the experimental group, so that a single liver tissue culture dish can be arrayed, and a large number of uniform liver tissue can be cultured at the same time. .

Therefore, the modularized cell culture system 1' can integrate the culture condition parameters such as temperature, carbon dioxide concentration, perfusion transport of culture solution, Ph value, dissolved oxygen amount and glucose concentration for comprehensive monitoring, and Integrate the required liquids, gases, light, and lines to regulate signals, and use system development technology to integrate the user interface to have a consistent modular system.

The above embodiments are intended to illustrate the principles of the disclosure and its functions, and are not intended to limit the disclosure. Any person skilled in the art can modify the above embodiments without departing from the spirit and scope of the disclosure. Therefore, the scope of protection of the present disclosure should be as set forth in the scope of the patent application described later.

1‧‧‧ cell culture system

10‧‧‧ Vehicles

10a‧‧‧ Carrier

10b‧‧‧temperature structure

100‧‧‧Electrical heating elements

100a‧‧‧heating rod

100b‧‧‧thermal clip

101‧‧‧Cover parts

11‧‧‧Lighting device

110‧‧‧Light source

110a‧‧‧Red light

110b‧‧‧Blue

9‧‧‧ Petri dishes

T‧‧‧ channel

Claims (12)

A cell culture system comprising: a carrier for carrying cells; and a light-emitting device connected to the carrier and having an adjustable wavelength light source, the light source being a light source for narrow-frequency images. The cell culture system according to claim 1, wherein the material for forming the carrier is iron or aluminum. The cell culture system according to claim 1, wherein the material forming the carrier is a light transmissive material. The cell culture system according to claim 1, wherein the carrier comprises a carrier for carrying cells, and a temperature regulating structure for thermally conducting the carrier. The cell culture system of claim 4, wherein the thermostatic structure comprises an electrothermal heating element contacting the carrier. The cell culture system according to claim 4, wherein the temperature-fixing structure comprises a covering member surrounding the carrier member, and the covering member has a passage between the covering member and the carrier member. The cell culture system of claim 1, wherein the carrier has a magnetic component. The cell culture system of claim 1, wherein the light source comprises blue light. The cell culture system of claim 1, wherein the light source comprises red light. The cell culture system according to claim 1, further comprising a liquid control device for communicating the carrier in a flow-through manner. The cell culture system according to claim 1, further comprising a gas control device that communicates the carrier in a gas-like manner. The cell culture system of claim 1, wherein the cell culture system has a plurality of the carriers, and the carrier arrays are arranged.