TW201322928A - Dietary stuff composition with weight reduction function - Google Patents

Abstract

The invention applies lactobacillus plantarum and glycostate wild bitter melon on animal experiments that include evaluation experiments of animal's weight changes, fat cell sizes and hemoglobin improvements inside mice. With a mixture of lactobacillus plantarum and glycostate wild bitter melon extracted powder, it is proved that growth promotion effect on lactic acid bacteria of mouse intestine can be surely attained, concentrations of cholesterol and triglyceride in blood can be improved, content of leptin can be obviously lowered, fat tissue weight and cell size can be obviously reduced, and outstanding efficacies of improving blood limpid and bowel can be obtained.

Description

Food and drink composition with weight reduction function

The present invention relates to a food and drink composition having a weight loss function, and more particularly to a food and drink composition comprising Lactobacillus plantarum and wild type Glycostate.

According to the current market demand for lactic acid bacteria products for the whole intestine and allergies, in recent years, some manufacturers have claimed that lactic acid bacteria have weight loss effects, but there is no empirical data from the actual credibility research unit, most of them are marketed by the stars or the public. public. In addition, commercially available lactic acid bacteria products are combined with other healthy food ingredients, such as: camellia, green tea, chitin, aloe vera, red peony or oligosaccharides, to enhance the appeal function, but in this composite formula Effective active ingredients, like the interaction of drugs, can also cause competition and inhibition, which may lead to enhanced, weakened, or even ineffective product effects.

Furthermore, abnormal blood fat is one of the main causes of cardiovascular disease and atherosclerosis (Hunninghake, 2001), in addition, Triglycerides (TG), High Density Lipoprotein cholesterol (High Density Lipoprotein cholesterol, The concentrations of HDL-c) and low density lipoprotein cholesterol (LDL-c) are also strongly correlated with cardiovascular disease (Pekkanenet al., 1990; Manson et al., 1992). Clinically, the concentration of cholesterol in the blood can be used as a good predictor. According to the National Cholesterol Education Program (NCEP), it is defined that the total cholesterol in the blood is 240 mg/dl, which is too high, ranging from 200 to 239. Mg/dl is called marginal hypercholesterolemia, and below 200 mg/dl is a more acceptable concentration range. This means that those with high cholesterol levels will have an increased chance of developing cardiovascular disease and an increase in mortality. When cholesterol in the blood is reduced by 1%, it will reduce the incidence of cardiovascular disease by 2 to 3% (Manson et al., 1992).

In view of the above, how to provide a food and beverage composition which can effectively lower the concentration of cholesterol and triglyceride in blood is the primary subject of the present invention, so the inventor of the present invention has been continually pondering and experimenting. Finally, there is the production of the present invention.

The main object of the present invention is to provide a food and drink composition having a weight-reducing function, which has a remarkable effect on improving blood lipids and the whole intestine.
In order to achieve the above object, the present invention provides a food and drink composition having a weight loss function, which is mainly composed of Lactobacillus plantarum and wild type Glycostate.
Preferably, the Lactobacillus plantarum accounts for 20-30% by weight of the food and beverage composition, and the wild type Glycostate comprises 70-80% by weight of the food and beverage composition. .
Preferably, the present invention further comprises Lactobacillus rhamnosus, Streptococcus thermophlius, Lactobacillus paracasei, and Lactobacillus fermentum in the food and beverage composition. And forming a compound probiotic with the Lactobacillus plantarum, and the composite probiotics account for 20-30% by weight of the composition of the food and beverage, and the wild type of bitter gourd extract (Glycostate) accounts for food and drink. The weight ratio of the composition is 70 to 80%.
The above and other objects and advantages of the present invention will be readily understood from
Of course, the invention may be varied on certain components, or in the arrangement of the components, but the selected embodiments are described in detail in the specification and their construction is shown in the drawings.

The present invention provides a food and drink composition having a weight loss function, which is mainly composed of a composite probiotic containing Lactobacillus plantarum and a wild type Glycostate, which accounts for Lactobacillus plantarum. The weight ratio of the food composition is 20 to 30%, and the wild type of Glycostate is 70 to 80% by weight of the composition of the food and beverage; the Lactobacillus plantarum is stored in the food. Industrial Development Institute, registration number BCRC 910472.
Preferably, the composite probiotic is composed of Lactobacillus plantarum, Lactobacillus rhamnosus, Streptococcus thermophlius, Lactobacillus paracasei, and Lactobacillus. The composition of fermentum is 20 to 30% by weight of the composite probiotics in the food and beverage composition, and the weight ratio of the wild type Glycostate to the composition of the food and beverage is 70 to 80%.
Hereinafter, the present invention and its achievable effects will be further described by way of an experimental example with a result chart.
In vivo testing of animals that improve blood lipids (1) Feeding mice that have induced hypercholesterolemia:
Male mice of the C57BL6/J strain used in the experiment were purchased from the National Laboratory Animal Breeding and Research Center. 7~8 weeks old, randomized, 6 rats in each group, fed separately in isolated cage (IVC), maintained at 25 °C at room temperature, and 12 hours light and dark at regular intervals; feed and sterile reverse osmosis water were allowed Free feeding, and during the week of adaptation to the environment, the feed was fed AIN-93 standard formula (Manufactured by MP Biomedicals Inc., France), but after the group was fed different proportions of feed, as detailed .
(2) Induced hypercholesterolemia and treatment grouping:
The feed formula fed after one week of feeding is mainly based on AIN-76. The ingredients include: casein, corn starch, corn oil, fiber, mineral mixture, vitamin mixture, sucrose, cholesterol and cholic acid. The ratio of soybean oil is increased to 15% and 0.5% of cholesterol is added to induce hypercholesterolemia. The time of feeding high cholesterol is 4 weeks, followed by 2 weeks of treatment, that is, the experimental group is added with lactic acid bacteria freeze-dried powder or wild type bitter gourd extract raw material. Or the two were mixed in standard feed, and the mice of each group were fed once a day at a fixed time. The mice were counted at an average of 25 g, and each feeding dose was 15 mg. The fed feed is stored in a 4 ° C refrigerator and fresh feed and sterile water are changed daily. Grouped as follows:

A: Negative control group - mice in this group were fed only with cholesterol supplement.
B: Drug control group - The mouse feed of this group was added with 2% of cholestyramine clinical drug in the feed, that is, about 0.3 mg of cholestyramine clinical drug in each daily feeding dose of 15 mg.
C: Wild type bitter gourd extract powder group - The bitter gourd extract powder was added to the feed at a concentration of 40%. That is, about 6 mg of bitter gourd extract powder is contained in 15 mg of each daily feeding dose.
D: composite probiotic group - only 10 ¹⁰ CFU / g of the lyophilized powder of the composite probiotic of the present invention, the final concentration is 1 × 10 ¹⁰ CFU / g and the AIN-93 standard formula of corn starch is appropriate Adjusted to contain about 1.5 mg of the composite probiotic freeze-dried powder in each of the daily feeding doses of 15 mg.
E: Experimental group of the present invention - the composite probiotic (10 ¹⁰ CFU/g) + wild type bitter gourd extract powder of the present invention is added to the feed, and in this experimental example, 1.5 mg is contained in each daily feeding dose of 15 mg. The composite probiotic freeze-dried powder and 6 mg of bitter gourd extract powder. Preferably, the weight ratio of the composite probiotic freeze-dried powder to the bitter gourd extract powder is: the composite probiotic freeze-dried powder accounts for about 20-30%, and the mountain bitter gourd extract powder accounts for about 70-80%. .
(3) Inspection and analysis items:
a. Weight measurement - mouse body weight was recorded weekly.
b. Blood analysis - The collected blood was centrifuged at 3000 rpm for 10 minutes at low speed, and the upper serum was aspirated and immediately frozen at -80 ° C until cholesterol and HDL, LDL analysis were performed, and the HDL/LDL ratio was calculated. The analytical method was performed using a blood biochemical analyzer, and leptin was analyzed by an immunoassay kit (ELISA kit).
c. Analysis of the distribution of probiotics in the feces and parts of the intestine and the number of surviving bacteria - after the 2 weeks of treatment, the feces of each mouse in each group were collected, weighed and sequenced in appropriate sterile PBS Dilute and analyze the number of lactic acid bacteria by Rogosa agar. In addition, 3 mice were randomly selected from the group fed with the probiotic group, and the digestive sites including the stomach, small intestine, large intestine and cecum were quickly cut out. After weighing separately, the group was homogenized and disrupted by 200 times by weight of sterile PBS, so that the bacteria in the digestive tract were fully released, and finally the lactic acid bacteria were counted continuously.
d. Adipose tissue analysis - analytical methods were modified after reference to the method of Hirsch et al. (1968). Abdominal adipose tissue was removed and fixed with osmium tetroxide, filtered through a 250 μm membrane filter paper, and washed with PBS to remove connective tissue, then the filtrate was removed at 25 μm, and the cells were fixed on filter paper, and 100 fats were randomly observed. The size of the cells is recorded and recorded.
(4) Biostatistics:
Each of the analysis items in this study was tested in three replicates. The results were statistically analyzed in the SPSS 10.0 software package on the computer. The one-way ANOVA (analysis of variance) was used to verify whether the treatment effect was significant. Differences were measured by Duncan's multiple range test. The difference between the significant differences was p<0.05, and the results were plotted with SigmaPlot 10.0 software.
(5) Test of inhibition of the invention by the food material:
The bacteriostatic ability of the lactic acid bacteria liquid was evaluated by the Well-agar diffusion method, and the procedure was referred to the method of Lin et al. (2006). Pour the sterilized MRS agar (Difco) into a bacterial culture dish. After solidification, a hole of 7 mm in diameter is punched on the culture dish with a sterile glass tube, and the compound probiotic liquid of the present invention cultured overnight is diluted to about 10 ^{7 .} Cfu/ml, evenly spread on a petri dish with a sterile cotton swab. After the bacteria solution is slightly dried, add 100 μl of various food materials (10%) dissolved in water to the holes. Do not allow the bacteria to overflow and cause errors. Each sample was subjected to three repetitions. Finally, the front side of the culture plate was placed in a 37 ° C incubator, and the diameter of the inhibition zone was measured after 16 to 18 hours.
(6) Storage stability test:
When the wild type bitter gourd extract powder (Glycostate) is mixed with the compound probiotic powder of the present invention, it shows no inhibition of lactic acid bacteria. Therefore, 10% of the wild type bitter gourd extract powder (Glycostate) and 10% of the compound probiotic powder of the present invention, Add 80% corn starch and other complexes, respectively, into a serum bottle and lock, then stored in a refrigerator at 4 ° C and room temperature (25 ± 3 ° C), and in an oven at 37 ° C, at 0 During the 30-day storage period, the number of viable bacteria in the lactic acid bacteria was sampled and analyzed.

Experimental results :
(1) Mouse body weight measurement:
Mouse body weights were recorded weekly. As a result, as shown in Fig. 1, the drug group and the experimental group of the present invention showed a significant decrease in body weight compared with the negative control group after 2 weeks of treatment. The wild type bitter gourd extract powder group also had a phenomenon of weight loss, but there was no statistically significant difference.
(2) Blood analysis:
The experimental results of blood lipid analysis, as shown in Fig. 2 and Fig. 3, showed that the cholesterol degradation effect of the feeding drug group was the best 36.2%; the second was the experimental group of the invention, which was 30.8%, and the simple feeding of the compound probiotic group There was also a significant decrease in cholesterol in mice, reaching 26.6%. In terms of the ratio of HDL to LDL cholesterol, it was found that the ratio of HDL/LDL of the feeding drug group and the experimental group of the present invention was high. Leptin was also found to be significantly reduced in both drug therapy and lactic acid bacteria administration groups (as shown in Figure 4). On the whole, the combined formula of the composite probiotic + wild type bitter gourd extract powder has a better hypolipidemic effect, thus demonstrating a synergistic effect.
(3) Analysis of the distribution of lactic acid bacteria and the number of viable bacteria in the feces and intestines:
The following table 1 and Figure 5 show the number of viable bacteria in the feces. From the results, it is clear that the mice in each group fed with lactic acid bacteria after 2 weeks of treatment have significantly higher lactic acid bacteria counts in the feces than those without lactic acid bacteria. It can be clearly seen from Fig. 5 that the composite probiotics of the present invention have a very good intestinal effect, and the number of fecal bacteria can be up to 9 Log CFU/g. Further, the digestive organs of various parts of the mouse were analyzed, and it was found that the composite probiotic of the present invention had a high concentration to survive in the large intestine and the cecum.
Table 1. Analysis of the number of lactic acid bacteria in the feces of mice after 2 weeks of treatment

(4) Fat cell analysis:
The fat cells were observed under a microscope and the size was calculated by a scale. The results showed that the fat cells were small in the feeding drug group and the lactic acid bacteria group, and 100 cells were statistically analyzed. The experimental results are shown in Fig. 6.
(5) Analysis of the inhibitory effect of food materials on lactic acid bacteria:
The selection of wild-type bitter gourd extract powder, camellia extract powder, hawthorn extract powder and aloe extract powder, which are commonly added to commercially available products, is tested for the effect of lactic acid bacteria activity after water re-dissolution. The results are shown in Table 2 below. As shown, it was shown that the mixed wild type bitter gourd extract powder and the hawthorn extract powder did not inhibit the growth of lactic acid bacteria against lactic acid bacteria.
Table 2. Inhibition of the composite probiotics of the present invention by different raw material extract powders


Note: The hole diameter on the medium is 7mm. "-" means that there is no inhibition of lactic acid bacteria growth.
(6) Analysis of storage stability test:
The powder is mixed with wild type bitter gourd extract powder or hawthorn extract powder and stored at different temperatures for 30 days. The results are shown in Table 3 below. The results show that the storage environment at 4 °C is the most appropriate, and the activity is almost no decrease, followed by It is stored at room temperature of 25 ° C, and the activity of the strain is greatly reduced at 37 ° C, so it is not suitable for high temperature storage.

As can be seen from the above experiments, the composition of the food and drink having the weight-reducing function composed of the compound probiotics and the wild type G. sylvestris extract (Glycostate) can synergistically promote the degradation of blood cholesterol and the leptin content. Relatively low, the ratio of HDL/LDL is increased, indicating that the present invention can effectively improve the proportion of cholesterol. The size of the fat cells was also observed in the adipose tissue of the abdominal cavity, and the fat cells fed to the lactic acid bacteria group were also significantly smaller.
Therefore, as a whole, the food and drink composition having the weight-reducing function of the present invention has a relatively good intestinal effect, and can be present in different parts of the digestive tract, thereby accelerating the degradation and excretion of cholesterol, thereby effectively improving blood lipids.
Furthermore, the invention can further enhance the acid resistance of the invention by using the freeze embedding drying technology, and has the tolerance of bile salt and the strong adsorption capacity, so that it can smoothly pass the stomach acid test and reach the intestinal tract for fixation and proliferation, and finally The expected effect of the whole intestine and cholesterol lowering was found, and it was found in the experiment that the present invention was mixed and stored with the wild probiotic bacteria containing Lactobacillus plantarum and the wild type bitter gourd extract (Glycostate), and stored at 25 ° C and 4 ° C. The bacterial activity is relatively stable, and therefore it can be used to prepare related preparations or foods such as powders or capsules.
The disclosure of the above-described embodiments is intended to be illustrative of the present invention and is not intended to limit the scope of the invention.
And the above detailed description will enable those skilled in the art to understand that the present invention can achieve the foregoing objects, and the present invention has significant progress in comparison with the products of the present invention, and has been in compliance with the provisions of the Patent Law. Therefore, the inventor of the case filed an application.

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Figure 1 is a graph showing the change in body weight of the animal test of the present invention.

Figure 2 is an analytical comparison diagram of the cholesterol degradation situation of the present invention.

Figure 3 is an analysis comparison of the ratio of HDL to LDL in the present invention.

Figure 4 is an analysis comparison chart of the leptin content of the present invention.

Figure 5 is a comparison chart of bacterial counts of animal feces lactic acid bacteria test according to the present invention.

Figure 6 is a comparative analysis of the cell size of abdominal adipose tissue according to the present invention.

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Claims (6)

A food and drink composition having a weight-reducing function, mainly composed of Lactobacillus plantarum and wild type Glycostate, which is deposited in the Food Industry Development Research Institute. The number is BCRC 910472. The food and drink composition having the weight reduction function according to the first aspect of the patent application, wherein the Lactobacillus plantarum accounts for 20 to 30% by weight of the food and beverage composition. The food and beverage composition having the weight reduction function according to the first aspect of the patent application, wherein the wild type of Glycostate comprises 70 to 80% by weight of the composition of the food and beverage. A food and drink composition having a weight-reducing function according to the first aspect of the patent application, wherein Lactobacillus rhamnosus, Streptococcus thermophlius, Lactobacillus paracasei are further added. And Lactobacillus fermentum, and a composite probiotic with the Lactobacillus plantarum. The food and beverage composition having the weight reduction function according to Item 4 of the patent application scope, wherein the compound probiotics account for 20 to 30% by weight of the composition of the food and beverage. A food and drink composition having a weight-reducing function according to the fourth aspect of the patent application, wherein the wild type of Glycostate has a weight ratio of 70 to 80% of the composition of the food and beverage.